MD06G1220200.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012352
Physical Location & Seq
Forward (+)
35218151 .. 35219491
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1220200.v1.1.491

Sequence Viewer

Length: 681 bp
ATGTCTAGAAAGCCAGGATTAGTATATTCGTCGGATTCTGATGACGATGGAGTAATCCATGGTGCAAAGCTCTTCGGACGTGAAAGGCCAATATATCATGTTCTAGGAGGTGGAAAAGTGGCGGATGTGTTGTTGTGGAGGAATATATATGTATCAGCTGTCCTTTTAATTGCCATGACGGGAATCTGGTTTCTTTTCGAAGTTGTCGAGTACAATTTCGTGACGCTTCTCTGTCACATCTCGATTACCACATTGTTGGTAATCTTCATATGGCGCATGGCTGCAGAAATATTCAAATTTCCGCCGCCTATGGTACCTCAAATGATATTACATGAAAACACATTCAAACAAGTAGCTTCCACTGCCCATAAAGGATTCAACCACTTCTTATCAAGAGTTCTTGATAGTGCATGCGGAAGAGACTTCCCATTCTTCTTCTTGACAATCATTTGTCTCTATATTTTGTCTGTGATCGGAAACTATTTCAGCTTTCTGAATCTTCTTTACTTGGGGTTCATCTGCTTGGAAACGCTACCATTCCTCTACGAGCGATTTGAGGAAGATGTTGATCGTTTAGCTGGAAAAATCAGCCGAGAGATAAAGAGATCGTACAGAAAGTTTGATTCTCAGTTTCTCAACAAAATTCCTAGAGGTCCAGTGAAAGACAAGAAAATCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

227

Amino Acids

26.13

Weight (kDa)

9.14

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 40 - 195 1.7e-46 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012352)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18260
fragaria_vesca FvH4_5g16680 FvH4_5g16680
malus_domestica MD06G1220200.v1.1 MD14G1229700.v1.1
prunus_persica Prupe.5G224500_v2.0.a1
pyrus_communis pycom06g19640 pycom14g19120
rosa_chinensis RchiOBHm_Chr7g0179441
rosa_laevigata RLG00000005356
rosa_multiflora Rmu_co8409275.1_g000001
rosa_roxburghii Rroxscaffold_3G00274020
rosa_rugosa Rorug06G0424200 Rorug06G0424200
rosa_samantha Rh7AG023800 Rh7BG023400 Rh7CG024800 Rh7DG024300
rosa_wichuraiana Rw7G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 313
AciI CCGC 4 cut(s) 122, 302, 305, 414
AcsI RAATTY 2 cut(s) 296, 642
AfaI GTAC 3 cut(s) 212, 315, 611
AgsI TTSAA 3 cut(s) 295, 346, 379
AjiI CACGTC 1 cut(s) 80
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 5 cut(s) 70, 158, 356, 489, 578
AluI AGCT 5 cut(s) 70, 158, 356, 489, 578
Alw26I GTCTC 2 cut(s) 414, 458
AoxI GGCC 1 cut(s) 86
ApeKI GCWGC 1 cut(s) 281
ApoI RAATTY 2 cut(s) 296, 642
Asp718I GGTACC 1 cut(s) 313
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 653
AsuII TTCGAA 1 cut(s) 198
AvaII GGWCC 1 cut(s) 653
BanI GGYRCC 1 cut(s) 313
BbvI GCAGC 1 cut(s) 268
BccI CCATC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 414, 458
BfaI CTAG 3 cut(s) 6, 104, 648
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 2 cut(s) 282, 305
BlsI GCNGC 2 cut(s) 283, 306
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 653
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 653
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 15
Bpu14I TTCGAA 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 656
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 1 cut(s) 130
BseJI GATNNNNATC 1 cut(s) 567
BseMII CTCAG 1 cut(s) 641
BseNI ACTGG 1 cut(s) 656
BseXI GCAGC 1 cut(s) 268
BshFI GGCC 1 cut(s) 88
BshNI GGYRCC 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 414, 458
BsnI GGCC 1 cut(s) 88
Bsp119I TTCGAA 1 cut(s) 198
Bsp143I GATC 3 cut(s) 471, 568, 605
Bsp19I CCATGG 1 cut(s) 58
BspACI CCGC 4 cut(s) 122, 302, 305, 414
BspANI GGCC 1 cut(s) 88
BspCNI CTCAG 1 cut(s) 640
BspLI GGNNCC 1 cut(s) 315
BspMAI CTGCAG 1 cut(s) 286
BspQI GCTCTTC 1 cut(s) 77
BspT104I TTCGAA 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 313
BsrI ACTGG 1 cut(s) 656
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 3 cut(s) 471, 568, 605
BssT1I CCWWGG 1 cut(s) 58
Bst2UI CCWGG 1 cut(s) 15
Bst6I CTCTTC 2 cut(s) 77, 412
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 1 cut(s) 627
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 1 cut(s) 130
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 3 cut(s) 474, 571, 608
BstMAI GTCTC 2 cut(s) 414, 458
BstMBI GATC 3 cut(s) 471, 568, 605
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 268
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuRI GGCC 1 cut(s) 88
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 130
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 360, 663
Cac8I GCNNGC 1 cut(s) 412
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 653
CseI GACGC 1 cut(s) 232
Csp6I GTAC 3 cut(s) 211, 314, 610
CviAII CATG 6 cut(s) 59, 98, 175, 277, 332, 411
CviJI RGCY 9 cut(s) 13, 70, 88, 158, 281, 356, 489, 578, 591
CviKI_1 RGCY 9 cut(s) 13, 70, 88, 158, 281, 356, 489, 578, 591
CviQI GTAC 3 cut(s) 211, 314, 610
DdeI CTNAG 1 cut(s) 627
DpnI GATC 3 cut(s) 473, 570, 607
DpnII GATC 3 cut(s) 471, 568, 605
Eam1104I CTCTTC 2 cut(s) 77, 412
EarI CTCTTC 2 cut(s) 77, 412
EciI GGCGGA 2 cut(s) 137, 291
Eco130I CCWWGG 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 653
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 6 cut(s) 62, 101, 178, 280, 335, 414
FatI CATG 6 cut(s) 58, 97, 174, 276, 331, 410
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 2 cut(s) 282, 305
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 2 cut(s) 282, 305
FspBI CTAG 3 cut(s) 6, 104, 648
GlaI GCGC 1 cut(s) 275
GluI GCNGC 2 cut(s) 282, 305
HaeIII GGCC 1 cut(s) 88
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 276
Hin1II CATG 6 cut(s) 62, 101, 178, 280, 335, 414
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HinfI GANTC 5 cut(s) 35, 183, 375, 496, 623
Hpy188I TCNGA 6 cut(s) 34, 40, 77, 476, 495, 677
Hpy188III TCNNGA 6 cut(s) 6, 220, 241, 393, 401, 439
Hpy99I CGWCG 1 cut(s) 34
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 3 cut(s) 65, 284, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 1 cut(s) 627
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 6 cut(s) 62, 101, 178, 280, 335, 414
HspAI GCGC 1 cut(s) 274
KpnI GGTACC 1 cut(s) 317
Kzo9I GATC 3 cut(s) 471, 568, 605
LguI GCTCTTC 1 cut(s) 77
LpnPI CCDG 4 cut(s) 27, 172, 564, 669
Lsp1109I GCAGC 1 cut(s) 268
MaeI CTAG 3 cut(s) 6, 104, 648
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 2 cut(s) 220, 233
MalI GATC 3 cut(s) 473, 570, 607
MboI GATC 3 cut(s) 471, 568, 605
MboII GAAGA 7 cut(s) 64, 256, 424, 427, 429, 491, 572
MluCI AATT 4 cut(s) 168, 214, 296, 642
MmeI TCCRAC 1 cut(s) 12
MnlI CCTC 6 cut(s) 101, 132, 327, 550, 551, 644
MseI TTAA 1 cut(s) 167
MspA1I CMGCKG 1 cut(s) 158
MspR9I CCNGG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 362
NcoI CCATGG 1 cut(s) 58
NdeI CATATG 1 cut(s) 269
NdeII GATC 3 cut(s) 471, 568, 605
NlaIII CATG 6 cut(s) 62, 101, 178, 280, 335, 414
NlaIV GGNNCC 1 cut(s) 315
NmeAIII GCCGAG 1 cut(s) 617
NmuCI GTSAC 2 cut(s) 220, 233
NspI RCATGY 1 cut(s) 414
NspV TTCGAA 1 cut(s) 198
PaeI GCATGC 1 cut(s) 414
PciSI GCTCTTC 1 cut(s) 77
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 5 cut(s) 35, 183, 375, 496, 623
PkrI GCNGC 2 cut(s) 283, 306
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 315
PspPI GGNCC 1 cut(s) 653
PstI CTGCAG 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 3 cut(s) 212, 315, 611
RsaNI GTAC 3 cut(s) 211, 314, 610
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 2 cut(s) 282, 305
Sau3AI GATC 3 cut(s) 471, 568, 605
Sau96I GGNCC 1 cut(s) 653
ScrFI CCNGG 1 cut(s) 15
SetI ASST 9 cut(s) 72, 82, 112, 160, 319, 358, 491, 580, 655
SfcI CTRYAG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 1 cut(s) 653
SphI GCATGC 1 cut(s) 414
Sse9I AATT 4 cut(s) 168, 214, 296, 642
SsiI CCGC 4 cut(s) 122, 302, 305, 414
SspI AATATT 1 cut(s) 291
SspMI CTAG 3 cut(s) 6, 104, 648
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 58
TaiI ACGT 1 cut(s) 82
TaqI TCGA 3 cut(s) 198, 207, 242
TasI AATT 4 cut(s) 168, 214, 296, 642
TatI WGTACW 1 cut(s) 210
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 5 cut(s) 35, 183, 375, 496, 623
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 2 cut(s) 367, 663
TseFI GTSAC 2 cut(s) 220, 233
TseI GCWGC 1 cut(s) 281
Tsp45I GTSAC 2 cut(s) 220, 233
TspDTI ATGAA 3 cut(s) 256, 348, 505
TspRI CASTG 2 cut(s) 367, 663
VpaK11BI GGWCC 1 cut(s) 653
XapI RAATTY 2 cut(s) 296, 642
XbaI TCTAGA 1 cut(s) 5
XceI RCATGY 1 cut(s) 414
XspI CTAG 3 cut(s) 6, 104, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.