MD14G1229700.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012352
Physical Location & Seq
Forward (+)
31079527 .. 31080798
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1229700.v1.1.491

Sequence Viewer

Length: 681 bp
ATGCACAGAAAGCCGGGATTAGTGTACTCGTCCGATTCTGATGACAACGGTGAAATCCGCAGTGCAAAGTTATTCGGACGTGAAAGGCCGATTCATCATGTTCTTGGAGGCGGAAAAGTTGCGGATGTGTTGTTGTGGAGGAACCGAAATGTATCAGCTGCGCTTTTGATTGCCATGACGGTAATCTGGTTTCTTTTCGAGGTTGCCGAGTACAATTTCATGACGCTCTTCTGTCACATCTCGATTTCCACATTGATGGTAATCTTCATATGGCGTACGGCTGCAGAAATATTCGGATTTCGGCCGCCTATGATACCACAAATGATATTACATGAAAACACATTCAAACAAGTAGCTTCCACTGTCCATAAAAGATTAAACCACTTCATATCAAGGGTCCTTGACAGTGCAAGTGGAGGAGACTTCCCATTCTTCTTCTTGACAATCATCTGTCTCTATATTTTGTCTCTGATCGGAACCTACTTCAGCTCTCTGAATCTTCTTTACTTGGGGTTCATCTGCTTGGAAACGCTACCATTCCTCTACGAGCGATTTGAGGAAGACGTTGATCGTTTAGCCGGAAAAATCAGCAGAGAGATAAAGAGATCGTACAGAAAATTTGTTTCTCAGTTTCTCAACCAAATTCCTAGAGGTCCAGTGAAAGACAAGAAAATCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

227

Amino Acids

26.16

Weight (kDa)

9.54

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 40 - 195 3.3e-45 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012352)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18260
fragaria_vesca FvH4_5g16680 FvH4_5g16680
malus_domestica MD06G1220200.v1.1 MD14G1229700.v1.1
prunus_persica Prupe.5G224500_v2.0.a1
pyrus_communis pycom06g19640 pycom14g19120
rosa_chinensis RchiOBHm_Chr7g0179441
rosa_laevigata RLG00000005356
rosa_multiflora Rmu_co8409275.1_g000001
rosa_roxburghii Rroxscaffold_3G00274020
rosa_rugosa Rorug06G0424200 Rorug06G0424200
rosa_samantha Rh7AG023800 Rh7BG023400 Rh7CG024800 Rh7DG024300
rosa_wichuraiana Rw7G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 58, 111, 122, 305
AcoI YGGCCR 1 cut(s) 302
AcsI RAATTY 2 cut(s) 617, 642
AcuI CTGAAG 1 cut(s) 469
AfaI GTAC 4 cut(s) 26, 212, 277, 611
AgsI TTSAA 1 cut(s) 346
AjiI CACGTC 1 cut(s) 80
AluBI AGCT 3 cut(s) 158, 356, 489
AluI AGCT 3 cut(s) 158, 356, 489
Alw26I GTCTC 3 cut(s) 414, 458, 471
AoxI GGCC 2 cut(s) 86, 302
ApeKI GCWGC 2 cut(s) 158, 281
ApoI RAATTY 2 cut(s) 617, 642
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 2 cut(s) 397, 653
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 62
AvaII GGWCC 2 cut(s) 397, 653
BbsI GAAGAC 1 cut(s) 567
BbvI GCAGC 2 cut(s) 145, 268
BccI CCATC 1 cut(s) 250
BceAI ACGGC 1 cut(s) 294
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 3 cut(s) 414, 458, 471
BfaI CTAG 1 cut(s) 648
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 3 cut(s) 159, 282, 305
BlsI GCNGC 3 cut(s) 160, 283, 306
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 397, 653
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 397, 653
BmiI GGNNCC 3 cut(s) 143, 398, 478
BmrFI CCNGG 1 cut(s) 15
BpiI GAAGAC 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 15
BsaBI GATNNNNATC 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 656
Bse8I GATNNNNATC 1 cut(s) 260
BseGI GGATG 1 cut(s) 130
BseJI GATNNNNATC 1 cut(s) 260
BseMII CTCAG 1 cut(s) 641
BseNI ACTGG 1 cut(s) 656
BseRI GAGGAG 1 cut(s) 432
BseX3I CGGCCG 1 cut(s) 302
BseXI GCAGC 2 cut(s) 145, 268
Bsh1285I CGRYCG 1 cut(s) 305
BshFI GGCC 2 cut(s) 88, 304
BsiEI CGRYCG 1 cut(s) 305
BsiSI CCGG 2 cut(s) 14, 579
BsiWI CGTACG 1 cut(s) 275
BsmAI GTCTC 3 cut(s) 414, 458, 471
BsnI GGCC 2 cut(s) 88, 304
Bsp143I GATC 3 cut(s) 471, 568, 605
BspACI CCGC 4 cut(s) 58, 111, 122, 305
BspANI GGCC 2 cut(s) 88, 304
BspCNI CTCAG 1 cut(s) 640
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 3 cut(s) 143, 398, 478
BspMAI CTGCAG 1 cut(s) 286
BspQI GCTCTTC 1 cut(s) 233
BsrI ACTGG 1 cut(s) 656
BssMI GATC 3 cut(s) 471, 568, 605
Bst4CI ACNGT 4 cut(s) 50, 181, 364, 407
Bst6I CTCTTC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 627
BstF5I GGATG 1 cut(s) 130
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 3 cut(s) 474, 571, 608
BstMAI GTCTC 3 cut(s) 414, 458, 471
BstMBI GATC 3 cut(s) 471, 568, 605
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 145, 268
BstV2I GAAGAC 1 cut(s) 567
BstXI CCANNNNNNTGG 1 cut(s) 256
BstZI CGGCCG 1 cut(s) 302
BsuRI GGCC 2 cut(s) 88, 304
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 130
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 4 cut(s) 67, 360, 412, 663
CciI TCATGA 1 cut(s) 219
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 2 cut(s) 397, 653
CseI GACGC 1 cut(s) 232
Csp6I GTAC 4 cut(s) 25, 211, 276, 610
CviAII CATG 4 cut(s) 98, 175, 220, 332
CviJI RGCY 8 cut(s) 13, 88, 158, 281, 304, 356, 489, 578
CviKI_1 RGCY 8 cut(s) 13, 88, 158, 281, 304, 356, 489, 578
CviQI GTAC 4 cut(s) 25, 211, 276, 610
DdeI CTNAG 1 cut(s) 627
DpnI GATC 3 cut(s) 473, 570, 607
DpnII GATC 3 cut(s) 471, 568, 605
EaeI YGGCCR 1 cut(s) 302
EagI CGGCCG 1 cut(s) 302
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
EciI GGCGGA 1 cut(s) 126
EclXI CGGCCG 1 cut(s) 302
Eco47I GGWCC 2 cut(s) 397, 653
Eco52I CGGCCG 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 397
FaeI CATG 4 cut(s) 101, 178, 223, 335
FatI CATG 4 cut(s) 97, 174, 219, 331
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 3 cut(s) 159, 282, 305
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 3 cut(s) 159, 282, 305
FspBI CTAG 1 cut(s) 648
GlaI GCGC 1 cut(s) 162
GluI GCNGC 3 cut(s) 159, 282, 305
HaeIII GGCC 2 cut(s) 88, 304
HapII CCGG 2 cut(s) 14, 579
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 163
Hin1II CATG 4 cut(s) 101, 178, 223, 335
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 3 cut(s) 35, 91, 496
HpaII CCGG 2 cut(s) 14, 579
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 8 cut(s) 34, 40, 77, 296, 471, 476, 495, 677
Hpy188III TCNNGA 3 cut(s) 220, 241, 439
Hpy8I GTNNAC 1 cut(s) 25
HpyCH4III ACNGT 4 cut(s) 50, 181, 364, 407
HpyCH4IV ACGT 2 cut(s) 79, 564
HpyCH4V TGCA 4 cut(s) 4, 65, 284, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 627
HpySE526I ACGT 2 cut(s) 79, 564
Hsp92II CATG 4 cut(s) 101, 178, 223, 335
HspAI GCGC 1 cut(s) 161
Kzo9I GATC 3 cut(s) 471, 568, 605
LguI GCTCTTC 1 cut(s) 233
LpnPI CCDG 4 cut(s) 27, 172, 592, 669
Lsp1109I GCAGC 2 cut(s) 145, 268
MaeI CTAG 1 cut(s) 648
MaeII ACGT 2 cut(s) 79, 564
MaeIII GTNAC 1 cut(s) 233
MalI GATC 3 cut(s) 473, 570, 607
MboI GATC 3 cut(s) 471, 568, 605
MboII GAAGA 6 cut(s) 220, 256, 424, 427, 491, 572
MluCI AATT 3 cut(s) 214, 617, 642
MnlI CCTC 7 cut(s) 101, 132, 193, 410, 550, 551, 644
MseI TTAA 1 cut(s) 377
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 2 cut(s) 14, 579
MspR9I CCNGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 269
NdeII GATC 3 cut(s) 471, 568, 605
NlaIII CATG 4 cut(s) 101, 178, 223, 335
NlaIV GGNNCC 3 cut(s) 143, 398, 478
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 233
PagI TCATGA 1 cut(s) 219
PciSI GCTCTTC 1 cut(s) 233
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 3 cut(s) 35, 91, 496
Pfl23II CGTACG 1 cut(s) 275
PkrI GCNGC 3 cut(s) 160, 283, 306
PpuMI RGGWCCY 1 cut(s) 397
Psp5II RGGWCCY 1 cut(s) 397
PspLI CGTACG 1 cut(s) 275
PspN4I GGNNCC 3 cut(s) 143, 398, 478
PspPI GGNCC 2 cut(s) 397, 653
PspPPI RGGWCCY 1 cut(s) 397
PstI CTGCAG 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 4 cut(s) 26, 212, 277, 611
RsaNI GTAC 4 cut(s) 25, 211, 276, 610
RseI CAYNNNNRTG 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 377
SatI GCNGC 3 cut(s) 159, 282, 305
Sau3AI GATC 3 cut(s) 471, 568, 605
Sau96I GGNCC 2 cut(s) 397, 653
ScrFI CCNGG 1 cut(s) 15
SetI ASST 8 cut(s) 82, 160, 204, 358, 482, 491, 567, 655
SfcI CTRYAG 1 cut(s) 282
SinI GGWCC 2 cut(s) 397, 653
SmiMI CAYNNNNRTG 1 cut(s) 254
Sse9I AATT 3 cut(s) 214, 617, 642
SsiI CCGC 4 cut(s) 58, 111, 122, 305
SspI AATATT 1 cut(s) 291
SspMI CTAG 1 cut(s) 648
StyD4I CCNGG 1 cut(s) 13
TaaI ACNGT 4 cut(s) 50, 181, 364, 407
TaiI ACGT 2 cut(s) 82, 567
TaqI TCGA 2 cut(s) 198, 242
TasI AATT 3 cut(s) 214, 617, 642
TatI WGTACW 2 cut(s) 24, 210
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 3 cut(s) 35, 91, 496
Tru1I TTAA 1 cut(s) 377
Tru9I TTAA 1 cut(s) 377
TscAI CASTG 4 cut(s) 67, 367, 412, 663
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 2 cut(s) 158, 281
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 6 cut(s) 83, 208, 256, 348, 376, 505
TspRI CASTG 4 cut(s) 67, 367, 412, 663
VpaK11BI GGWCC 2 cut(s) 397, 653
XapI RAATTY 2 cut(s) 617, 642
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.