FvH4_5g17080

exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010147
Physical Location & Seq
Forward (+)
9735790 .. 9740150
4361 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17080.t2

Sequence Viewer

Length: 1737 bp
ATGGGCATCAAAGATCTCCTCAGATTCATCAAACCCTACATTCAAATCATCCATATCAGGAAATACGCTGGCAAGCGAGTGGGCATTGATGCCTATTCATGGCTTCACAAAGGAGCTTATTCATGTAGCATGGAGCTGTGTATGAATTCGAATAGCGATAGGAAGTTGAAATACATTGACTATTTTATGCACCGAATACATATGCTTCAGCACCATGAGATAACCCCAGTTGTGGTTTTTGATGGTGGCAATGTACCATGTAAGGCTGCCACTGGGGATGAACGCCACAGGAGGAGGAAGAAGTTTCATGATTTGGCCATGGAGAAAGTAGGAGAAGGGAATGTTAGAGCTGCCACTGAGCTCTTTCAGAGGGCGGTGAGTGTAACTCCATCTATGGCACATGAGCTGATAAAGGTTCTGAGATCAGAGAACATTGAATTTGTTGTAGCTCCATATGAGGCTGATGCGCAGCTAGCATACCTGGCCAGTCTTGAAGCAGAAAAGGGTGGAATTGCGGCAGTCATCACTGAGGATAGTGATCTAGTGGCATATGGCTGCCAAGCTATAATCTTTAAGATGGACCAATTCGGCAATGGTGATGAGTTACTGCTAGACAATATTTTTAATCAAGTGGACCGCACACCTTCCTTCCAAAATTTTGACCTCAAATTATTCACAGGTATGTGTGTCTTGGCTGGCTGCGATTTCCTTCCATCTGTACCTGGAATTGGAATGGGTAAAGCTTATGCCTTGGTTTCTAAGTACCAAAATTTAGATCGCGCTTTATCAGTTTTGAAATTACAGAAGGGCAATCAAATGCCTGAAGATTACCCCAAGTCTTTCAGAGAAGCAGCTGCAGTTTTCCAGCATGCTCAAGTATATGACTCTGACAAGAAAATTGTCAAGCATATGAGACCGATACCACAAGATCTTGTGGATTCCCTTGATGGAGAAATTGATTTCCTTGGACCAGAAATCCCTTCATCAATAGCCATTGCAATTGCTGAAGGAAAATTGGACCCCATAAGTATGGAGGCCTTTGATCATTTCCCCAGTTCCAGTTCTAGTCATCGTTCAGGTCCCATTAAGAGCCAAATTACTCACCACTTCCAGAGATGTGAGACTGCTATTGTATCTCCACAAGTCAGTAGCTTTGTGGCCTTTTCCTCCCATAAAACCAGAAAACAAGATAGTCCTATGCCTACAGAAAAGCATAATCTAGTTTTAGACGAGAAGAAGTATTTGAAGGAAGCTGCAGCACTTGGAAAACTAATTGTTCCTTCGACAAACAATGAAGAGGTGAAAAGTACCATAGTGCCCAACAATATCCCCTTGAAGGTTCGTGCCAGCAACCCTTTCAAGAAAATGAAACAGGAAATCAATTCGGATCAAATAGGTAGCAATACTAAGCAAGTTTCAATTGCAAGTGAGGTAGAGATATTGGACACCTTGTGTGCCTCTACCAGTAACATATCTTCAATAGTTCTCGATAGCCCTAGAAAAAGAAAAAACGATATTGACTTAGATGACATAGAGAGTGAGAGTAGTCAGGAACTAGTTTCTGTGGTGACAAGTACACAGATAGAACCTTCAGGCATGCCGACTTCAGACTCTCAGGAGAGTGTAAATTCTAAGCTATCCAAGCAAAAGAGGATAGTGAAAAATGACAAGTTGAAGAGAAACAGCTGTTCAACAAATAAGAAAGGCAGTATCTTGAATTTCTTTTCACCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

579

Amino Acids

64.31

Weight (kDa)

7.62

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XPG_N PF00752 1 - 101 1.3e-20 XPG N-terminal domain
XPG_I PF00867 144 - 235 1.4e-14 XPG I-region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18090 AT1G18090
fragaria_vesca FvH4_5g17080 FvH4_5g17080 FvH4_5g17080 FvH4_5g17080 FvH4_5g17080
malus_domestica MD14G1234100.v1.1
prunus_persica Prupe.5G230300_v2.0.a1
pyrus_communis pycom14g19510
rosa_chinensis RchiOBHm_Chr7g0178771
rosa_laevigata RLG00000005417
rosa_multiflora Rmu_sc0015029.1_g000001
rosa_roxburghii Rroxscaffold_3G00274630
rosa_rugosa Rorug06G0419200
rosa_samantha Rh7AG018700 Rh7AG018800 Rh7BG018100 Rh7CG018900 Rh7DG018700
rosa_wichuraiana Rw7G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 468
AccII CGCG 1 cut(s) 780
AciI CCGC 3 cut(s) 374, 515, 637
AclWI GGATC 1 cut(s) 1395
AcoI YGGCCR 2 cut(s) 315, 483
AcsI RAATTY 6 cut(s) 145, 437, 655, 769, 1627, 1717
AcuI CTGAAG 5 cut(s) 191, 843, 1026, 1575, 1590
AdeI CACNNNGTG 1 cut(s) 1452
AfaI GTAC 5 cut(s) 255, 720, 764, 1309, 1576
AfiI CCNNNNNNNGG 4 cut(s) 99, 232, 728, 1336
AhlI ACTAGT 1 cut(s) 1555
AjnI CCWGG 2 cut(s) 480, 721
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 907, 1115
AlwI GGATC 1 cut(s) 1395
AoxI GGCC 4 cut(s) 315, 483, 1035, 1158
ApeKI GCWGC 9 cut(s) 266, 350, 469, 555, 699, 851, 854, 1253, 1256
ApoI RAATTY 6 cut(s) 145, 437, 655, 769, 1627, 1717
AspLEI GCGC 2 cut(s) 469, 782
AspS9I GGNCC 5 cut(s) 580, 634, 968, 1018, 1079
AsuHPI GGTGA 6 cut(s) 388, 608, 1094, 1312, 1579, 1719
AsuII TTCGAA 1 cut(s) 149
AsuNHI GCTAGC 1 cut(s) 472
AvaII GGWCC 5 cut(s) 580, 634, 968, 1018, 1079
BaeGI GKGCMC 1 cut(s) 1320
BalI TGGCCA 2 cut(s) 317, 485
BanII GRGCYC 1 cut(s) 363
BarI GAAGNNNNNNTAC 4 cut(s) 155, 187, 1459, 1491
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 9 cut(s) 253, 337, 481, 542, 686, 841, 863, 1240, 1268
BccI CCATC 5 cut(s) 236, 397, 571, 721, 941
BciT130I CCWGG 2 cut(s) 482, 723
BclI TGATCA 1 cut(s) 1042
BcoDI GTCTC 2 cut(s) 907, 1115
BcuI ACTAGT 1 cut(s) 1555
BfaI CTAG 7 cut(s) 473, 542, 611, 1065, 1220, 1497, 1556
BfmI CTRYAG 3 cut(s) 855, 1203, 1254
BglII AGATCT 2 cut(s) 13, 928
Bme1390I CCNGG 2 cut(s) 482, 723
Bme18I GGWCC 5 cut(s) 580, 634, 968, 1018, 1079
BmgT120I GGNCC 5 cut(s) 580, 634, 968, 1018, 1079
BmiI GGNNCC 2 cut(s) 1020, 1081
BmrFI CCNGG 2 cut(s) 482, 723
BmrI ACTGGG 3 cut(s) 221, 282, 1047
BmsI GCATC 3 cut(s) 15, 79, 454
BmtI GCTAGC 1 cut(s) 476
BmuI ACTGGG 3 cut(s) 221, 282, 1047
BplI GAGNNNNNCTC 2 cut(s) 370, 402
Bpu14I TTCGAA 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 858
BsaBI GATNNNNATC 1 cut(s) 537
BsaI GGTCTC 1 cut(s) 907
BsaJI CCNNGG 3 cut(s) 318, 750, 964
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 232, 728, 1336
Bse1I ACTGG 6 cut(s) 227, 277, 486, 1053, 1059, 1464
Bse3DI GCAATG 3 cut(s) 256, 598, 993
Bse8I GATNNNNATC 1 cut(s) 537
BseBI CCWGG 2 cut(s) 482, 723
BseDI CCNNGG 3 cut(s) 318, 750, 964
BseGI GGATG 2 cut(s) 48, 283
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 4 cut(s) 99, 232, 728, 1336
BseMI GCAATG 3 cut(s) 256, 598, 993
BseMII CTCAG 5 cut(s) 34, 348, 410, 519, 1628
BseNI ACTGG 6 cut(s) 227, 277, 486, 1053, 1059, 1464
BseRI GAGGAG 2 cut(s) 8, 307
BseSI GKGCMC 1 cut(s) 1320
BseXI GCAGC 9 cut(s) 253, 337, 481, 542, 686, 841, 863, 1240, 1268
Bsh1236I CGCG 1 cut(s) 780
BshFI GGCC 4 cut(s) 317, 485, 1037, 1160
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 4 cut(s) 99, 232, 728, 1336
BsmAI GTCTC 2 cut(s) 907, 1115
BsmFI GGGAC 1 cut(s) 1065
BsnI GGCC 4 cut(s) 317, 485, 1037, 1160
Bso31I GGTCTC 1 cut(s) 907
Bsp119I TTCGAA 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 363, 1320
Bsp143I GATC 7 cut(s) 13, 422, 538, 775, 928, 1042, 1387
Bsp19I CCATGG 1 cut(s) 318
BspACI CCGC 3 cut(s) 374, 515, 637
BspANI GGCC 4 cut(s) 317, 485, 1037, 1160
BspCNI CTCAG 5 cut(s) 33, 349, 411, 520, 1627
BspFNI CGCG 1 cut(s) 780
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 2 cut(s) 1020, 1081
BspMAI CTGCAG 2 cut(s) 859, 1258
BspOI GCTAGC 1 cut(s) 476
BspPI GGATC 1 cut(s) 1395
BspT104I TTCGAA 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 907
BsrDI GCAATG 3 cut(s) 256, 598, 993
BsrI ACTGG 6 cut(s) 227, 277, 486, 1053, 1059, 1464
BssECI CCNNGG 3 cut(s) 318, 750, 964
BssMI GATC 7 cut(s) 13, 422, 538, 775, 928, 1042, 1387
BssT1I CCWWGG 3 cut(s) 318, 750, 964
Bst2UI CCWGG 2 cut(s) 482, 723
Bst6I CTCTTC 2 cut(s) 1290, 1670
BstBI TTCGAA 1 cut(s) 149
BstC8I GCNNGC 7 cut(s) 70, 74, 474, 697, 870, 1348, 1598
BstDEI CTNAG 9 cut(s) 20, 357, 419, 528, 759, 1407, 1522, 1614, 1632
BstDSI CCRYGG 1 cut(s) 318
BstF5I GGATG 2 cut(s) 48, 283
BstFNI CGCG 1 cut(s) 780
BstHHI GCGC 2 cut(s) 469, 782
BstKTI GATC 7 cut(s) 16, 425, 541, 778, 931, 1045, 1390
BstMAI GTCTC 2 cut(s) 907, 1115
BstMBI GATC 7 cut(s) 13, 422, 538, 775, 928, 1042, 1387
BstMWI GCNNNNNNNGC 3 cut(s) 473, 482, 1642
BstNI CCWGG 2 cut(s) 482, 723
BstNSI RCATGY 2 cut(s) 872, 1600
BstSCI CCNGG 2 cut(s) 480, 721
BstSFI CTRYAG 3 cut(s) 855, 1203, 1254
BstSLI GKGCMC 1 cut(s) 1320
BstUI CGCG 1 cut(s) 780
BstV1I GCAGC 9 cut(s) 253, 337, 481, 542, 686, 841, 863, 1240, 1268
BstX2I RGATCY 2 cut(s) 13, 928
BstXI CCANNNNNNTGG 1 cut(s) 1030
BstYI RGATCY 2 cut(s) 13, 928
BsuRI GGCC 4 cut(s) 317, 485, 1037, 1160
BtgI CCRYGG 1 cut(s) 318
BtsCI GGATG 2 cut(s) 48, 283
BtsIMutI CAGTG 3 cut(s) 270, 354, 525
Cac8I GCNNGC 7 cut(s) 70, 74, 474, 697, 870, 1348, 1598
CciI TCATGA 1 cut(s) 307
CfoI GCGC 2 cut(s) 469, 782
Cfr13I GGNCC 5 cut(s) 580, 634, 968, 1018, 1079
Csp6I GTAC 5 cut(s) 254, 719, 763, 1308, 1575
CviQI GTAC 5 cut(s) 254, 719, 763, 1308, 1575
DdeI CTNAG 9 cut(s) 20, 357, 419, 528, 759, 1407, 1522, 1614, 1632
DpnI GATC 7 cut(s) 15, 424, 540, 777, 930, 1044, 1389
DpnII GATC 7 cut(s) 13, 422, 538, 775, 928, 1042, 1387
DraIII CACNNNGTG 1 cut(s) 1452
EaeI YGGCCR 2 cut(s) 315, 483
Eam1104I CTCTTC 2 cut(s) 1290, 1670
EarI CTCTTC 2 cut(s) 1290, 1670
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 3 cut(s) 318, 750, 964
Eco147I AGGCCT 1 cut(s) 1037
Eco24I GRGCYC 1 cut(s) 363
Eco31I GGTCTC 1 cut(s) 907
Eco47I GGWCC 5 cut(s) 580, 634, 968, 1018, 1079
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 5 cut(s) 191, 843, 1026, 1575, 1590
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 1079
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 480, 721
EcoT14I CCWWGG 3 cut(s) 318, 750, 964
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 3 cut(s) 318, 750, 964
FaqI GGGAC 1 cut(s) 1065
FauNDI CATATG 4 cut(s) 201, 454, 550, 909
FbaI TGATCA 1 cut(s) 1042
FokI GGATG 2 cut(s) 35, 290
FriOI GRGCYC 1 cut(s) 363
FspBI CTAG 7 cut(s) 473, 542, 611, 1065, 1220, 1497, 1556
FspI TGCGCA 1 cut(s) 468
GlaI GCGC 2 cut(s) 468, 781
HaeIII GGCC 4 cut(s) 317, 485, 1037, 1160
HhaI GCGC 2 cut(s) 469, 782
Hin6I GCGC 2 cut(s) 467, 780
HinP1I GCGC 2 cut(s) 467, 780
HindIII AAGCTT 1 cut(s) 741
HinfI GANTC 4 cut(s) 24, 884, 938, 1610
HphI GGTGA 6 cut(s) 388, 608, 1094, 1312, 1579, 1719
Hpy166II GTNNAC 2 cut(s) 634, 1577
Hpy188I TCNGA 8 cut(s) 23, 369, 420, 427, 845, 889, 1387, 1609
Hpy188III TCNNGA 9 cut(s) 58, 308, 491, 1111, 1360, 1487, 1550, 1616, 1714
Hpy8I GTNNAC 2 cut(s) 634, 1577
HpyCH4V TGCA 5 cut(s) 190, 857, 998, 1256, 1424
HpyF10VI GCNNNNNNNGC 3 cut(s) 473, 482, 1642
HpyF3I CTNAG 9 cut(s) 20, 357, 419, 528, 759, 1407, 1522, 1614, 1632
HspAI GCGC 2 cut(s) 467, 780
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 7 cut(s) 13, 422, 538, 775, 928, 1042, 1387
LmnI GCTCC 3 cut(s) 113, 133, 454
Lsp1109I GCAGC 9 cut(s) 253, 337, 481, 542, 686, 841, 863, 1240, 1268
LweI GCATC 3 cut(s) 15, 79, 454
MaeI CTAG 7 cut(s) 473, 542, 611, 1065, 1220, 1497, 1556
MaeIII GTNAC 4 cut(s) 382, 603, 1466, 1567
MalI GATC 7 cut(s) 15, 424, 540, 777, 930, 1044, 1389
MboI GATC 7 cut(s) 13, 422, 538, 775, 928, 1042, 1387
MboII GAAGA 6 cut(s) 310, 836, 1246, 1307, 1467, 1687
MfeI CAATTG 2 cut(s) 999, 1419
MflI RGATCY 2 cut(s) 13, 928
MhlI GDGCHC 2 cut(s) 363, 1320
MlsI TGGCCA 2 cut(s) 317, 485
MluNI TGGCCA 2 cut(s) 317, 485
MlyI GAGTC 2 cut(s) 878, 1604
Mox20I TGGCCA 2 cut(s) 317, 485
MscI TGGCCA 2 cut(s) 317, 485
MseI TTAA 3 cut(s) 573, 624, 1086
MslI CAYNNNNRTG 3 cut(s) 680, 1028, 1732
Msp20I TGGCCA 2 cut(s) 317, 485
MspA1I CMGCKG 2 cut(s) 854, 1686
MspR9I CCNGG 2 cut(s) 482, 723
MunI CAATTG 2 cut(s) 999, 1419
MvaI CCWGG 2 cut(s) 482, 723
MvnI CGCG 1 cut(s) 780
MwoI GCNNNNNNNGC 3 cut(s) 473, 482, 1642
NcoI CCATGG 1 cut(s) 318
NdeI CATATG 4 cut(s) 201, 454, 550, 909
NdeII GATC 7 cut(s) 13, 422, 538, 775, 928, 1042, 1387
NheI GCTAGC 1 cut(s) 472
NlaIV GGNNCC 2 cut(s) 1020, 1081
NmuCI GTSAC 1 cut(s) 1567
NsbI TGCGCA 1 cut(s) 468
NspI RCATGY 2 cut(s) 872, 1600
NspV TTCGAA 1 cut(s) 149
PaeI GCATGC 2 cut(s) 872, 1600
PagI TCATGA 1 cut(s) 307
PceI AGGCCT 1 cut(s) 1037
PfeI GAWTC 2 cut(s) 24, 938
PleI GAGTC 2 cut(s) 878, 1604
PpsI GAGTC 2 cut(s) 878, 1604
PpuMI RGGWCCY 1 cut(s) 1079
Psp124BI GAGCTC 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 1079
Psp6I CCWGG 2 cut(s) 480, 721
PspGI CCWGG 2 cut(s) 480, 721
PspN4I GGNNCC 2 cut(s) 1020, 1081
PspPI GGNCC 5 cut(s) 580, 634, 968, 1018, 1079
PspPPI RGGWCCY 1 cut(s) 1079
PstI CTGCAG 2 cut(s) 859, 1258
PsuI RGATCY 2 cut(s) 13, 928
PvuII CAGCTG 2 cut(s) 854, 1686
RsaI GTAC 5 cut(s) 255, 720, 764, 1309, 1576
RsaNI GTAC 5 cut(s) 254, 719, 763, 1308, 1575
RseI CAYNNNNRTG 3 cut(s) 680, 1028, 1732
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 3 cut(s) 573, 624, 1086
Sau3AI GATC 7 cut(s) 13, 422, 538, 775, 928, 1042, 1387
Sau96I GGNCC 5 cut(s) 580, 634, 968, 1018, 1079
SchI GAGTC 2 cut(s) 878, 1604
ScrFI CCNGG 2 cut(s) 482, 723
SduI GDGCHC 2 cut(s) 363, 1320
SfaNI GCATC 3 cut(s) 15, 79, 454
SfcI CTRYAG 3 cut(s) 855, 1203, 1254
SfuI TTCGAA 1 cut(s) 149
SinI GGWCC 5 cut(s) 580, 634, 968, 1018, 1079
SmiMI CAYNNNNRTG 3 cut(s) 680, 1028, 1732
SmlI CTYRAG 1 cut(s) 873
SmoI CTYRAG 1 cut(s) 873
SpeI ACTAGT 1 cut(s) 1555
SphI GCATGC 2 cut(s) 872, 1600
SseBI AGGCCT 1 cut(s) 1037
SsiI CCGC 3 cut(s) 374, 515, 637
SspI AATATT 1 cut(s) 619
SspMI CTAG 7 cut(s) 473, 542, 611, 1065, 1220, 1497, 1556
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 1037
StyD4I CCNGG 2 cut(s) 480, 721
StyI CCWWGG 3 cut(s) 318, 750, 964
TaqI TCGA 3 cut(s) 149, 1283, 1488
TaqII GACCGA 1 cut(s) 931
TatI WGTACW 1 cut(s) 1574
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 2 cut(s) 24, 938
Tru1I TTAA 3 cut(s) 573, 624, 1086
Tru9I TTAA 3 cut(s) 573, 624, 1086
TscAI CASTG 3 cut(s) 277, 361, 532
TseFI GTSAC 1 cut(s) 1567
TseI GCWGC 9 cut(s) 266, 350, 469, 555, 699, 851, 854, 1253, 1256
Tsp45I GTSAC 1 cut(s) 1567
TspDTI ATGAA 9 cut(s) 16, 87, 111, 158, 294, 296, 972, 1308, 1382
TspRI CASTG 3 cut(s) 277, 361, 532
VpaK11BI GGWCC 5 cut(s) 580, 634, 968, 1018, 1079
XapI RAATTY 6 cut(s) 145, 437, 655, 769, 1627, 1717
XceI RCATGY 2 cut(s) 872, 1600
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XspI CTAG 7 cut(s) 473, 542, 611, 1065, 1220, 1497, 1556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.