Prupe.5G230300_v2.0.a1

exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0010147
Physical Location & Seq
Forward (+)
17593946 .. 17597964
4019 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G230300.1

Sequence Viewer

Length: 1737 bp
ATGGGTATCAAAGATCTTCTCAGATTCATGAAACCTTATATTTCACCCATTCATATCAAGAAATATGCCGGCAAGCGCGTGGGTATCGACGCCTATTCATGGCTTCACAAAGGAGCATATTCATGTAGTTTGGAGCTTTGTATGAATTCAAATAGCGAAAGGAAATTGAAATACATAGATTATTTTATGCACCGAATAAATCTGCTTCGTCACCATAAGATAACCCCAGTAGTCGTCTTTGATGGTGGCAATGTACCATGTAAGGCTGCCACTGCAGAAGAGCGCCACAGGAGAAGAAAGACTAATCATGATTTGGCAATGGCAAAATTTAAGGAAGGGGATATTAGAGCTGCCACTGAGCTCTTCCAGAGGGCAGTGAGTGTAACTCCATCTATGGCACATCAACTAATACAGATTCTGAGATCAGAGAATATTGAATTTGTAGTGGCTCCATATGAGGCTGATGCACAGCTAGCATTCCTGTCCAATCTCGAAGCAGAGAAGGGTGGAATTGCTGCAGTTATTACAGAGGATAGTGATCTAGTGGCATATGGTTGCCAAGCTGTTGTCTTTAAGATGGACCAATATGGTAACGGTGAAGAGATGGTGCTGGAGAATGTTTTTAATCCAGTGGGCCGTACACCTTCCTTCCAAAATTTTGATCAGGAATTATTCACGGGTATGTGTGTCTTGGCAGGCTGTGATTTCCTTCCATCTGTTCATGGTATTGGGATTGCAAAAGCTTATGCCTTGGTTTCTAAGTATCGTAACTTAGATCGTGCTCTATCAGTTTTGAAATCGCAGAAGGGCAATCAAATGCCTGAAGATTACCCCAAATGTTTCAAAGAAGCAGTTGCAGTTTTTCAGCATGCTCAAATATTTGATGCCGAAAAGAAAAAGGTCAAACATATGAAAGCCATTCCAGAAAACCTTGTGCAGTCTCTAGATGGAGAACTTGATTTCCTCGGACCAGAAATGCCTCCATCAGTAGCTACTGCAATTGCTGAAGGAAACTTGGACCCCATAAATATGAAGGCATTTGATCATTTCCCAGCCTCTAGACATCATCAAGAACCTATTAAGAGCCAAAATATTGGTCAAATTCAGAGACCTGAGACTGCTGTGGTAGCTGCAGAAGATAGCTGTTTTGTAAGCTTTCCCTCCCATAAAAGCCGAAGGAAAGATAGTACAGGGCCTACAGAAAGGCAGAATCCTGTTTCAAATGAAAATAAGTATTTGAATGAATCTGCAGCACTTGCAAAGCTAATTGTGCCATCGACAAATAATGTAACACTGGAGAGCACAATAGCACCCAACACCATCCCGTTGAAGGTTCGCCACAACAATCCATTCAAGAAACGGAAAGATGATGTTCACTCAGATCAAATGGGGAGCAAAACTAAACAAGTTTCAAGTGAAGCTGAGGTGGAAATTTCGGACACCTTGAATGTCTCTCCCAGTAACATCAAAACTAAAGTTGTTAACAATGTTCCTTCCAGAAAAAGGAAAAATGATATTATCTTAGAGCATGTAGAAAGCATTGGGGAAGAGGTTTCAGTGGTGACAGAGATAGAGTCTACAGACATATTGTGTGTAACTCCAGAGTCCCAGGAAAGTGTAAACTCTAAGCTTCCCAAGGGAAAAAGGATAGTGAAAAATGAGAAGTTGAAGAGAAGCAGCTTCAAGAGTTCAGGGAATAAAAATAGCAGTATCTTGAATTTCTTTTCTCGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

579

Amino Acids

64.5

Weight (kDa)

8.79

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18090 AT1G18090
fragaria_vesca FvH4_5g17080 FvH4_5g17080 FvH4_5g17080 FvH4_5g17080 FvH4_5g17080
malus_domestica MD14G1234100.v1.1
prunus_persica Prupe.5G230300_v2.0.a1
pyrus_communis pycom14g19510
rosa_chinensis RchiOBHm_Chr7g0178771
rosa_laevigata RLG00000005417
rosa_multiflora Rmu_sc0015029.1_g000001
rosa_roxburghii Rroxscaffold_3G00274630
rosa_rugosa Rorug06G0419200
rosa_samantha Rh7AG018700 Rh7AG018800 Rh7BG018100 Rh7CG018900 Rh7DG018700
rosa_wichuraiana Rw7G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1577
AccII CGCG 1 cut(s) 78
AcsI RAATTY 7 cut(s) 145, 326, 437, 655, 1101, 1431, 1717
AcuI CTGAAG 2 cut(s) 843, 1026
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 3 cut(s) 255, 640, 1189
AfiI CCNNNNNNNGG 3 cut(s) 99, 1330, 1503
AjnI CCWGG 1 cut(s) 1608
AloI GAACNNNNNNTCC 2 cut(s) 945, 977
Alw21I GWGCWC 3 cut(s) 363, 784, 1304
Alw26I GTCTC 4 cut(s) 945, 1102, 1109, 1456
AlwNI CAGNNNCTG 1 cut(s) 418
AoxI GGCC 2 cut(s) 634, 1193
ApeKI GCWGC 6 cut(s) 266, 350, 515, 1130, 1250, 1677
ApoI RAATTY 7 cut(s) 145, 326, 437, 655, 1101, 1431, 1717
ArsI GACNNNNNNTTYG 2 cut(s) 1081, 1113
AspLEI GCGC 2 cut(s) 78, 285
AspS9I GGNCC 5 cut(s) 580, 634, 968, 1018, 1193
AsuHPI GGTGA 4 cut(s) 36, 203, 608, 1573
AsuNHI GCTAGC 1 cut(s) 472
AvaII GGWCC 3 cut(s) 580, 968, 1018
BanII GRGCYC 1 cut(s) 363
BauI CACGAG 1 cut(s) 1728
Bbv12I GWGCWC 3 cut(s) 363, 784, 1304
BbvCI CCTCAGC 1 cut(s) 1422
BbvI GCAGC 6 cut(s) 253, 337, 502, 1117, 1262, 1689
BccI CCATC 9 cut(s) 236, 397, 571, 598, 721, 941, 991, 1282, 1328
BceAI ACGGC 1 cut(s) 621
BciT130I CCWGG 1 cut(s) 1610
BclI TGATCA 2 cut(s) 661, 1042
BcoDI GTCTC 4 cut(s) 945, 1102, 1109, 1456
BfaI CTAG 4 cut(s) 473, 542, 944, 1059
BfmI CTRYAG 6 cut(s) 273, 516, 1131, 1197, 1248, 1578
BfoI RGCGCY 1 cut(s) 286
BglII AGATCT 1 cut(s) 13
BisI GCNGC 6 cut(s) 267, 351, 516, 1131, 1251, 1678
BlsI GCNGC 6 cut(s) 268, 352, 517, 1132, 1252, 1679
Bme1390I CCNGG 1 cut(s) 1610
Bme18I GGWCC 3 cut(s) 580, 968, 1018
BmgT120I GGNCC 5 cut(s) 580, 634, 968, 1018, 1193
BmiI GGNNCC 2 cut(s) 450, 1020
BmrFI CCNGG 1 cut(s) 1610
BmrI ACTGGG 2 cut(s) 221, 1452
BmsI GCATC 2 cut(s) 454, 874
BmtI GCTAGC 1 cut(s) 476
BmuI ACTGGG 2 cut(s) 221, 1452
BplI GAGNNNNNCTC 2 cut(s) 370, 402
BpmI CTGGAG 3 cut(s) 632, 1316, 1584
Bpu10I CCTNAGC 1 cut(s) 1422
BsaBI GATNNNNATC 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 1102
BsaJI CCNNGG 4 cut(s) 750, 964, 1608, 1635
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 1330, 1503
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 4 cut(s) 227, 629, 1299, 1458
Bse3DI GCAATG 2 cut(s) 256, 324
Bse8I GATNNNNATC 1 cut(s) 537
BseBI CCWGG 1 cut(s) 1610
BseDI CCNNGG 4 cut(s) 750, 964, 1608, 1635
BseGI GGATG 1 cut(s) 1320
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 3 cut(s) 99, 1330, 1503
BseMI GCAATG 2 cut(s) 256, 324
BseMII CTCAG 6 cut(s) 34, 348, 410, 1104, 1392, 1413
BseNI ACTGG 4 cut(s) 227, 629, 1299, 1458
BseXI GCAGC 6 cut(s) 253, 337, 502, 1117, 1262, 1689
BseYI CCCAGC 1 cut(s) 1051
BsgI GTGCAG 1 cut(s) 956
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 2 cut(s) 636, 1195
BsiHKAI GWGCWC 3 cut(s) 363, 784, 1304
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 1591
BslI CCNNNNNNNGG 3 cut(s) 99, 1330, 1503
BsmAI GTCTC 4 cut(s) 945, 1102, 1109, 1456
BsmFI GGGAC 1 cut(s) 1591
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 2 cut(s) 636, 1195
Bso31I GGTCTC 1 cut(s) 1102
Bsp1286I GDGCHC 3 cut(s) 363, 784, 1304
Bsp143I GATC 7 cut(s) 13, 422, 538, 661, 775, 1042, 1381
BspANI GGCC 2 cut(s) 636, 1195
BspCNI CTCAG 6 cut(s) 33, 349, 411, 1105, 1391, 1414
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 2 cut(s) 27, 307
BspLI GGNNCC 2 cut(s) 450, 1020
BspMAI CTGCAG 4 cut(s) 277, 520, 1135, 1252
BspOI GCTAGC 1 cut(s) 476
BspQI GCTCTTC 2 cut(s) 273, 368
BspTNI GGTCTC 1 cut(s) 1102
BsrDI GCAATG 2 cut(s) 256, 324
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 4 cut(s) 227, 629, 1299, 1458
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 4 cut(s) 750, 964, 1608, 1635
BssMI GATC 7 cut(s) 13, 422, 538, 661, 775, 1042, 1381
BssNI GRCGYC 1 cut(s) 90
BssSI CACGAG 1 cut(s) 1728
BssT1I CCWWGG 2 cut(s) 750, 1635
Bst2BI CACGAG 1 cut(s) 1728
Bst2UI CCWGG 1 cut(s) 1610
Bst4CI ACNGT 1 cut(s) 596
Bst6I CTCTTC 5 cut(s) 273, 368, 594, 1542, 1664
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstC8I GCNNGC 5 cut(s) 70, 74, 474, 697, 870
BstF5I GGATG 1 cut(s) 1320
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 1 cut(s) 286
BstHHI GCGC 2 cut(s) 78, 285
BstKTI GATC 7 cut(s) 16, 425, 541, 664, 778, 1045, 1384
BstMAI GTCTC 4 cut(s) 945, 1102, 1109, 1456
BstMBI GATC 7 cut(s) 13, 422, 538, 661, 775, 1042, 1381
BstMWI GCNNNNNNNGC 5 cut(s) 272, 473, 1127, 1256, 1270
BstNI CCWGG 1 cut(s) 1610
BstNSI RCATGY 2 cut(s) 872, 1532
BstSCI CCNGG 1 cut(s) 1608
BstSFI CTRYAG 6 cut(s) 273, 516, 1131, 1197, 1248, 1578
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 6 cut(s) 253, 337, 502, 1117, 1262, 1689
BstX2I RGATCY 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 1094
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 2 cut(s) 636, 1195
BtsCI GGATG 1 cut(s) 1320
BtsI GCAGTG 2 cut(s) 270, 381
BtsIMutI CAGTG 6 cut(s) 270, 354, 381, 636, 1292, 1563
Cac8I GCNNGC 5 cut(s) 70, 74, 474, 697, 870
CaiI CAGNNNCTG 1 cut(s) 418
CciI TCATGA 2 cut(s) 27, 307
CfoI GCGC 2 cut(s) 78, 285
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 5 cut(s) 580, 634, 968, 1018, 1193
CseI GACGC 1 cut(s) 98
Csp6I GTAC 3 cut(s) 254, 639, 1188
CviAII CATG 8 cut(s) 28, 99, 123, 258, 308, 722, 869, 1529
CviQI GTAC 3 cut(s) 254, 639, 1188
DpnI GATC 7 cut(s) 15, 424, 540, 663, 777, 1044, 1383
DpnII GATC 7 cut(s) 13, 422, 538, 661, 775, 1042, 1381
Eam1104I CTCTTC 5 cut(s) 273, 368, 594, 1542, 1664
EarI CTCTTC 5 cut(s) 273, 368, 594, 1542, 1664
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 2 cut(s) 750, 1635
Eco24I GRGCYC 1 cut(s) 363
Eco31I GGTCTC 1 cut(s) 1102
Eco47I GGWCC 3 cut(s) 580, 968, 1018
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 2 cut(s) 843, 1026
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 1193
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 1608
EcoT14I CCWWGG 2 cut(s) 750, 1635
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 2 cut(s) 750, 1635
FaeI CATG 8 cut(s) 31, 102, 126, 261, 311, 725, 872, 1532
FaqI GGGAC 1 cut(s) 1591
FatI CATG 8 cut(s) 27, 98, 122, 257, 307, 721, 868, 1528
FauNDI CATATG 3 cut(s) 454, 550, 909
FbaI TGATCA 2 cut(s) 661, 1042
FblI GTMKAC 1 cut(s) 1577
Fnu4HI GCNGC 6 cut(s) 267, 351, 516, 1131, 1251, 1678
FokI GGATG 1 cut(s) 1307
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 6 cut(s) 267, 351, 516, 1131, 1251, 1678
FspBI CTAG 4 cut(s) 473, 542, 944, 1059
GlaI GCGC 2 cut(s) 77, 284
GluI GCNGC 6 cut(s) 267, 351, 516, 1131, 1251, 1678
GsaI CCCAGC 1 cut(s) 1055
GsuI CTGGAG 3 cut(s) 632, 1316, 1584
HaeII RGCGCY 1 cut(s) 286
HaeIII GGCC 2 cut(s) 636, 1195
HapII CCGG 1 cut(s) 69
HgaI GACGC 1 cut(s) 98
HhaI GCGC 2 cut(s) 78, 285
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 8 cut(s) 31, 102, 126, 261, 311, 725, 872, 1532
Hin6I GCGC 2 cut(s) 76, 283
HinP1I GCGC 2 cut(s) 76, 283
HincII GTYRAC 1 cut(s) 1483
HindII GTYRAC 1 cut(s) 1483
HindIII AAGCTT 3 cut(s) 741, 1153, 1628
HinfI GANTC 6 cut(s) 24, 415, 1210, 1244, 1574, 1604
HpaI GTTAAC 1 cut(s) 1483
HpaII CCGG 1 cut(s) 69
HphI GGTGA 4 cut(s) 36, 203, 608, 1573
Hpy166II GTNNAC 5 cut(s) 641, 1375, 1483, 1578, 1621
Hpy188I TCNGA 7 cut(s) 23, 420, 427, 968, 1107, 1381, 1438
Hpy8I GTNNAC 5 cut(s) 641, 1375, 1483, 1578, 1621
Hpy99I CGWCG 1 cut(s) 92
HpyCH4III ACNGT 1 cut(s) 596
HpyF10VI GCNNNNNNNGC 5 cut(s) 272, 473, 1127, 1256, 1270
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 8 cut(s) 31, 102, 126, 261, 311, 725, 872, 1532
HspAI GCGC 2 cut(s) 76, 283
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Ksp22I TGATCA 2 cut(s) 661, 1042
KspAI GTTAAC 1 cut(s) 1483
Kzo9I GATC 7 cut(s) 13, 422, 538, 661, 775, 1042, 1381
LguI GCTCTTC 2 cut(s) 273, 368
LmnI GCTCC 4 cut(s) 113, 133, 454, 1392
Lsp1109I GCAGC 6 cut(s) 253, 337, 502, 1117, 1262, 1689
LweI GCATC 2 cut(s) 454, 874
MaeI CTAG 4 cut(s) 473, 542, 944, 1059
MaeIII GTNAC 8 cut(s) 209, 382, 590, 767, 1288, 1460, 1561, 1594
MalI GATC 7 cut(s) 15, 424, 540, 663, 777, 1044, 1383
MboI GATC 7 cut(s) 13, 422, 538, 661, 775, 1042, 1381
MboII GAAGA 9 cut(s) 8, 290, 306, 355, 611, 836, 1148, 1559, 1681
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 3 cut(s) 363, 784, 1304
MlyI GAGTC 2 cut(s) 1583, 1613
MnlI CCTC 9 cut(s) 363, 451, 523, 974, 990, 1066, 1171, 1417, 1543
MroNI GCCGGC 1 cut(s) 68
MseI TTAA 5 cut(s) 330, 573, 624, 1080, 1482
MslI CAYNNNNRTG 3 cut(s) 121, 680, 1028
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 1610
MunI CAATTG 1 cut(s) 999
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 1 cut(s) 1610
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 5 cut(s) 272, 473, 1127, 1256, 1270
NaeI GCCGGC 1 cut(s) 70
NdeI CATATG 3 cut(s) 454, 550, 909
NdeII GATC 7 cut(s) 13, 422, 538, 661, 775, 1042, 1381
NgoMIV GCCGGC 1 cut(s) 68
NheI GCTAGC 1 cut(s) 472
NlaIII CATG 8 cut(s) 31, 102, 126, 261, 311, 725, 872, 1532
NlaIV GGNNCC 2 cut(s) 450, 1020
NmuCI GTSAC 2 cut(s) 209, 1561
NspI RCATGY 2 cut(s) 872, 1532
PaeI GCATGC 1 cut(s) 872
PagI TCATGA 2 cut(s) 27, 307
PciSI GCTCTTC 2 cut(s) 273, 368
PctI GAATGC 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 70
PfeI GAWTC 4 cut(s) 24, 415, 1210, 1244
PkrI GCNGC 6 cut(s) 268, 352, 517, 1132, 1252, 1679
PleI GAGTC 2 cut(s) 1582, 1612
PpsI GAGTC 2 cut(s) 1582, 1612
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 1608
PspFI CCCAGC 1 cut(s) 1051
PspGI CCWGG 1 cut(s) 1608
PspN4I GGNNCC 2 cut(s) 450, 1020
PspPI GGNCC 5 cut(s) 580, 634, 968, 1018, 1193
PstI CTGCAG 4 cut(s) 277, 520, 1135, 1252
PstNI CAGNNNCTG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 3 cut(s) 255, 640, 1189
RsaNI GTAC 3 cut(s) 254, 639, 1188
RseI CAYNNNNRTG 3 cut(s) 121, 680, 1028
SacI GAGCTC 1 cut(s) 363
SapI GCTCTTC 2 cut(s) 273, 368
SaqAI TTAA 5 cut(s) 330, 573, 624, 1080, 1482
SatI GCNGC 6 cut(s) 267, 351, 516, 1131, 1251, 1678
Sau3AI GATC 7 cut(s) 13, 422, 538, 661, 775, 1042, 1381
Sau96I GGNCC 5 cut(s) 580, 634, 968, 1018, 1193
SchI GAGTC 2 cut(s) 1583, 1613
ScrFI CCNGG 1 cut(s) 1610
SduI GDGCHC 3 cut(s) 363, 784, 1304
SfaNI GCATC 2 cut(s) 454, 874
SfcI CTRYAG 6 cut(s) 273, 516, 1131, 1197, 1248, 1578
SinI GGWCC 3 cut(s) 580, 968, 1018
SmiMI CAYNNNNRTG 3 cut(s) 121, 680, 1028
SphI GCATGC 1 cut(s) 872
SspI AATATT 3 cut(s) 433, 879, 1093
SspMI CTAG 4 cut(s) 473, 542, 944, 1059
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 1 cut(s) 1608
StyI CCWWGG 2 cut(s) 750, 1635
TaaI ACNGT 1 cut(s) 596
TaqI TCGA 3 cut(s) 87, 492, 1277
TatI WGTACW 1 cut(s) 1187
TfiI GAWTC 4 cut(s) 24, 415, 1210, 1244
Tru1I TTAA 5 cut(s) 330, 573, 624, 1080, 1482
Tru9I TTAA 5 cut(s) 330, 573, 624, 1080, 1482
TscAI CASTG 6 cut(s) 277, 361, 381, 636, 1299, 1563
TseFI GTSAC 2 cut(s) 209, 1561
TseI GCWGC 6 cut(s) 266, 350, 515, 1130, 1250, 1677
Tsp45I GTSAC 2 cut(s) 209, 1561
TspGWI ACGGA 1 cut(s) 1375
TspRI CASTG 6 cut(s) 277, 361, 381, 636, 1299, 1563
VpaK11BI GGWCC 3 cut(s) 580, 968, 1018
XapI RAATTY 7 cut(s) 145, 326, 437, 655, 1101, 1431, 1717
XbaI TCTAGA 2 cut(s) 943, 1058
XceI RCATGY 2 cut(s) 872, 1532
XmiI GTMKAC 1 cut(s) 1577
XspI CTAG 4 cut(s) 473, 542, 944, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.