Rroxscaffold_3G00274630

exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010147
Physical Location & Seq
Forward (+)
66243919 .. 66248253
4335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274630.1

Sequence Viewer

Length: 1356 bp
ATGGGCATCAAAGATCTCCTCAGATTCATCAAACCCTACATTCAAATCATTCATATCAAGAAATACGCCGGCAAACGAGTGGGCATTGATGCCTATTCATGGCTTCACAAAGGAGGTATTAAAGTTTTGAGAAGGAAGAAATTTCATGATTTGGCAATGGAAAAAGTAGAAGAAGGGAATGTTAGAGCTGCCACCGAGCTCTTTCAGAGGGCAGTGAGCGTAACTCCATCTATGGCACATGAGCTGATAAAGGTTCTGAGATCAGAGAACATTGAATTTGTTGTAGCTCCATATGAGGCTGATGCGCAGCTAGCATTCCTGGCCAGTCTTGAAGCAGAAAAGGGTGGAATTGCGGCAGTCATCACTGAGGATAGTGATCTAGTGGCATATGGCTGCCAAGCTATAATCTTTAAGATGGACCAATTCGGCAACGGCGACGAGTTACTGCTGGACAATGTTTTTAATCAAGTGGACCGCACACCTTCCTTCCAAAATTTTGACCTCGAATTATTTACAGGTATGTGTGTCTTGGCTGGCTGTGATTTCCTTCCATCTGTTCCTGGAATCGGGATTGGTAAAGCTTATGCCTTGGTTTCTAAGTATCAAAACTTAGATCGTGCTTTATCAATTTTGAAACTGCAGAAGGGCAATCAAATGCCTGAAGATTACCCCAAATCTTTCAGAGAAGCCACTGCAGTTTTCCAGCATGCACAAGTATACGACTCTGACAAGAAAATTGTCAAGCATATGAGACCCATACCACAAGAGCTTGTGGAGTCCCTTGATGGAGAACTTGATTTCCTGGGACCAGGAATCCCTTCATCAATAGCTATTGCAATTGCTGAAGGAAAATTGGACCCCATAAGTATGGAGGCCTTTGATCATTTCCCCAGTTCCAGTTCTAGACATCGTTCAGATATTATTAAGAGCCAAATTACTCATCACTTTCAGAGATCTGAGACTGCTACTGTATCTGCACAAGTCAGTAGCTTTGTGGCCTTTTCCTCCCATAAAACCAGAAAACAAGATAAAAAGCAGAATCCAGTTACAGATGAAAAGAAGTATTTGAATGAAGCTGCAGCACTTGGAAAACTAATTGTTCCTTCGACAAAGAATGAAGTGGTGGAAAGTACCACAGTTTCCAACAATATCCCGTTGAAGGTTCGGGCCAGCAATCCTTTCAAGAAAAGGAAACAGGAAACCAACTCGGATCAAATAGTGAGCAACACTGAGCAAGTTTCAATTGCAAGTGGGGTAGAGATATTGGACACCTTGTGTGCCTCTACCAGTAACATATCTTCAACAGTTCTCGACGATAGCCCCAGAAAAAGAAAAAAACGATGTCCACTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

451

Amino Acids

50.07

Weight (kDa)

8.57

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XPG_I PF00867 90 - 181 2.8e-14 XPG I-region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18090 AT1G18090
fragaria_vesca FvH4_5g17080 FvH4_5g17080 FvH4_5g17080 FvH4_5g17080 FvH4_5g17080
malus_domestica MD14G1234100.v1.1
prunus_persica Prupe.5G230300_v2.0.a1
pyrus_communis pycom14g19510
rosa_chinensis RchiOBHm_Chr7g0178771
rosa_laevigata RLG00000005417
rosa_multiflora Rmu_sc0015029.1_g000001
rosa_roxburghii Rroxscaffold_3G00274630
rosa_rugosa Rorug06G0419200
rosa_samantha Rh7AG018700 Rh7AG018800 Rh7BG018100 Rh7CG018900 Rh7DG018700
rosa_wichuraiana Rw7G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 306
AccI GTMKAC 1 cut(s) 717
AciI CCGC 2 cut(s) 353, 475
AclWI GGATC 1 cut(s) 1218
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 3 cut(s) 140, 275, 493
AcuI CTGAAG 2 cut(s) 681, 864
AdeI CACNNNGTG 1 cut(s) 1275
AfaI GTAC 1 cut(s) 1132
AfiI CCNNNNNNNGG 3 cut(s) 99, 566, 1159
AgsI TTSAA 9 cut(s) 44, 275, 332, 634, 1069, 1159, 1183, 1242, 1302
AjnI CCWGG 4 cut(s) 318, 559, 801, 808
AloI GAACNNNNNNTCC 2 cut(s) 783, 815
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 2 cut(s) 745, 953
AlwI GGATC 1 cut(s) 1218
AoxI GGCC 4 cut(s) 321, 873, 996, 1167
ApeKI GCWGC 5 cut(s) 188, 307, 393, 1076, 1079
ApoI RAATTY 3 cut(s) 140, 275, 493
Asp700I GAANNNNTTC 1 cut(s) 817
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 5 cut(s) 418, 472, 806, 856, 1167
AsuNHI GCTAGC 1 cut(s) 310
AvaII GGWCC 4 cut(s) 418, 472, 806, 856
BalI TGGCCA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 1282, 1314
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 5 cut(s) 175, 319, 380, 1063, 1091
BccI CCATC 4 cut(s) 235, 409, 559, 779
BceAI ACGGC 1 cut(s) 448
BciT130I CCWGG 4 cut(s) 320, 561, 803, 810
BclI TGATCA 1 cut(s) 880
BcoDI GTCTC 2 cut(s) 745, 953
BfaI CTAG 3 cut(s) 311, 380, 903
BfmI CTRYAG 3 cut(s) 638, 693, 1077
BglII AGATCT 2 cut(s) 13, 953
BisI GCNGC 6 cut(s) 189, 308, 354, 394, 1077, 1080
BlsI GCNGC 6 cut(s) 190, 309, 355, 395, 1078, 1081
Bme1390I CCNGG 4 cut(s) 320, 561, 803, 810
Bme18I GGWCC 4 cut(s) 418, 472, 806, 856
BmgT120I GGNCC 5 cut(s) 418, 472, 806, 856, 1167
BmiI GGNNCC 2 cut(s) 807, 858
BmrFI CCNGG 4 cut(s) 320, 561, 803, 810
BmrI ACTGGG 1 cut(s) 885
BmsI GCATC 3 cut(s) 15, 79, 292
BmtI GCTAGC 1 cut(s) 314
BmuI ACTGGG 1 cut(s) 885
BplI GAGNNNNNCTC 2 cut(s) 208, 240
BsaBI GATNNNNATC 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 745
BsaJI CCNNGG 2 cut(s) 588, 802
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 566, 1159
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 5 cut(s) 324, 891, 897, 1043, 1287
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 4 cut(s) 320, 561, 803, 810
BseDI CCNNGG 2 cut(s) 588, 802
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 3 cut(s) 99, 566, 1159
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 5 cut(s) 34, 248, 357, 948, 1221
BseNI ACTGG 5 cut(s) 324, 891, 897, 1043, 1287
BseRI GAGGAG 1 cut(s) 8
BseXI GCAGC 5 cut(s) 175, 319, 380, 1063, 1091
BsgI GTGCAG 1 cut(s) 960
BshFI GGCC 4 cut(s) 323, 875, 998, 1169
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 2 cut(s) 763, 819
BslI CCNNNNNNNGG 3 cut(s) 99, 566, 1159
BsmAI GTCTC 2 cut(s) 745, 953
BsmFI GGGAC 2 cut(s) 763, 819
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 4 cut(s) 323, 875, 998, 1169
Bso31I GGTCTC 1 cut(s) 745
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 7 cut(s) 13, 260, 376, 613, 880, 953, 1210
BspACI CCGC 2 cut(s) 353, 475
BspANI GGCC 4 cut(s) 323, 875, 998, 1169
BspCNI CTCAG 5 cut(s) 33, 249, 358, 949, 1222
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 807, 858
BspMAI CTGCAG 3 cut(s) 642, 697, 1081
BspOI GCTAGC 1 cut(s) 314
BspPI GGATC 1 cut(s) 1218
BspTNI GGTCTC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 5 cut(s) 324, 891, 897, 1043, 1287
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 588, 802
BssMI GATC 7 cut(s) 13, 260, 376, 613, 880, 953, 1210
BssNAI GTATAC 1 cut(s) 718
BssT1I CCWWGG 1 cut(s) 588
Bst1107I GTATAC 1 cut(s) 718
Bst2UI CCWGG 4 cut(s) 320, 561, 803, 810
Bst4CI ACNGT 3 cut(s) 970, 1138, 1306
BstC8I GCNNGC 5 cut(s) 70, 312, 535, 708, 1171
BstDEI CTNAG 8 cut(s) 20, 257, 366, 597, 610, 957, 1230, 1349
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 7 cut(s) 16, 263, 379, 616, 883, 956, 1213
BstMAI GTCTC 2 cut(s) 745, 953
BstMBI GATC 7 cut(s) 13, 260, 376, 613, 880, 953, 1210
BstMWI GCNNNNNNNGC 2 cut(s) 311, 320
BstNI CCWGG 4 cut(s) 320, 561, 803, 810
BstNSI RCATGY 1 cut(s) 710
BstSCI CCNGG 4 cut(s) 318, 559, 801, 808
BstSFI CTRYAG 3 cut(s) 638, 693, 1077
BstV1I GCAGC 5 cut(s) 175, 319, 380, 1063, 1091
BstX2I RGATCY 2 cut(s) 13, 953
BstXI CCANNNNNNTGG 1 cut(s) 868
BstYI RGATCY 2 cut(s) 13, 953
BstZ17I GTATAC 1 cut(s) 718
BsuRI GGCC 4 cut(s) 323, 875, 998, 1169
BtsI GCAGTG 2 cut(s) 219, 690
BtsIMutI CAGTG 4 cut(s) 219, 363, 690, 1227
Cac8I GCNNGC 5 cut(s) 70, 312, 535, 708, 1171
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 5 cut(s) 418, 472, 806, 856, 1167
Csp6I GTAC 1 cut(s) 1131
CviAII CATG 4 cut(s) 99, 146, 239, 707
CviQI GTAC 1 cut(s) 1131
DdeI CTNAG 8 cut(s) 20, 257, 366, 597, 610, 957, 1230, 1349
DpnI GATC 7 cut(s) 15, 262, 378, 615, 882, 955, 1212
DpnII GATC 7 cut(s) 13, 260, 376, 613, 880, 953, 1210
DraIII CACNNNGTG 1 cut(s) 1275
EaeI YGGCCR 1 cut(s) 321
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 588
Eco147I AGGCCT 1 cut(s) 875
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 745
Eco47I GGWCC 4 cut(s) 418, 472, 806, 856
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 2 cut(s) 681, 864
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 4 cut(s) 318, 559, 801, 808
EcoT14I CCWWGG 1 cut(s) 588
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 588
FaeI CATG 4 cut(s) 102, 149, 242, 710
FaqI GGGAC 2 cut(s) 763, 819
FatI CATG 4 cut(s) 98, 145, 238, 706
FauNDI CATATG 3 cut(s) 292, 388, 747
FbaI TGATCA 1 cut(s) 880
FblI GTMKAC 1 cut(s) 717
Fnu4HI GCNGC 6 cut(s) 189, 308, 354, 394, 1077, 1080
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 6 cut(s) 189, 308, 354, 394, 1077, 1080
FspBI CTAG 3 cut(s) 311, 380, 903
FspI TGCGCA 1 cut(s) 306
GlaI GCGC 1 cut(s) 306
GluI GCNGC 6 cut(s) 189, 308, 354, 394, 1077, 1080
HaeIII GGCC 4 cut(s) 323, 875, 998, 1169
HapII CCGG 1 cut(s) 69
HhaI GCGC 1 cut(s) 307
Hin1II CATG 4 cut(s) 102, 149, 242, 710
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 6 cut(s) 24, 564, 722, 776, 813, 1039
HpaII CCGG 1 cut(s) 69
Hpy166II GTNNAC 3 cut(s) 472, 718, 1346
Hpy188III TCNNGA 7 cut(s) 58, 146, 329, 568, 903, 1183, 1310
Hpy8I GTNNAC 3 cut(s) 472, 718, 1346
Hpy99I CGWCG 2 cut(s) 440, 1316
HpyCH4III ACNGT 3 cut(s) 970, 1138, 1306
HpyCH4V TGCA 7 cut(s) 640, 695, 710, 836, 977, 1079, 1247
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 320
HpyF3I CTNAG 8 cut(s) 20, 257, 366, 597, 610, 957, 1230, 1349
Hsp92II CATG 4 cut(s) 102, 149, 242, 710
HspAI GCGC 1 cut(s) 305
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 7 cut(s) 13, 260, 376, 613, 880, 953, 1210
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 5 cut(s) 175, 319, 380, 1063, 1091
LweI GCATC 3 cut(s) 15, 79, 292
MaeI CTAG 3 cut(s) 311, 380, 903
MaeIII GTNAC 4 cut(s) 220, 441, 1045, 1289
MalI GATC 7 cut(s) 15, 262, 378, 615, 882, 955, 1212
MboI GATC 7 cut(s) 13, 260, 376, 613, 880, 953, 1210
MboII GAAGA 4 cut(s) 148, 182, 674, 1290
MfeI CAATTG 2 cut(s) 837, 1242
MflI RGATCY 2 cut(s) 13, 953
MhlI GDGCHC 1 cut(s) 201
MlsI TGGCCA 1 cut(s) 323
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 2 cut(s) 716, 785
MmeI TCCRAC 1 cut(s) 1167
MnlI CCTC 9 cut(s) 29, 107, 201, 289, 361, 512, 865, 1015, 1291
Mox20I TGGCCA 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 68
MroXI GAANNNNTTC 1 cut(s) 817
MscI TGGCCA 1 cut(s) 323
MseI TTAA 4 cut(s) 120, 411, 462, 924
MslI CAYNNNNRTG 2 cut(s) 866, 1351
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 4 cut(s) 320, 561, 803, 810
MunI CAATTG 2 cut(s) 837, 1242
Mva1269I GAATGC 1 cut(s) 314
MvaI CCWGG 4 cut(s) 320, 561, 803, 810
MwoI GCNNNNNNNGC 2 cut(s) 311, 320
NaeI GCCGGC 1 cut(s) 70
NdeI CATATG 3 cut(s) 292, 388, 747
NdeII GATC 7 cut(s) 13, 260, 376, 613, 880, 953, 1210
NgoMIV GCCGGC 1 cut(s) 68
NheI GCTAGC 1 cut(s) 310
NlaIII CATG 4 cut(s) 102, 149, 242, 710
NlaIV GGNNCC 2 cut(s) 807, 858
NsbI TGCGCA 1 cut(s) 306
NspI RCATGY 1 cut(s) 710
PaeI GCATGC 1 cut(s) 710
PagI TCATGA 1 cut(s) 145
PceI AGGCCT 1 cut(s) 875
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PctI GAATGC 1 cut(s) 314
PdiI GCCGGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 817
PfeI GAWTC 4 cut(s) 24, 564, 813, 1039
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 6 cut(s) 190, 309, 355, 395, 1078, 1081
PleI GAGTC 2 cut(s) 716, 784
PpsI GAGTC 2 cut(s) 716, 784
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 4 cut(s) 318, 559, 801, 808
PspGI CCWGG 4 cut(s) 318, 559, 801, 808
PspN4I GGNNCC 2 cut(s) 807, 858
PspPI GGNCC 5 cut(s) 418, 472, 806, 856, 1167
PstI CTGCAG 3 cut(s) 642, 697, 1081
PsuI RGATCY 2 cut(s) 13, 953
RsaI GTAC 1 cut(s) 1132
RsaNI GTAC 1 cut(s) 1131
RseI CAYNNNNRTG 2 cut(s) 866, 1351
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 4 cut(s) 120, 411, 462, 924
SatI GCNGC 6 cut(s) 189, 308, 354, 394, 1077, 1080
Sau3AI GATC 7 cut(s) 13, 260, 376, 613, 880, 953, 1210
Sau96I GGNCC 5 cut(s) 418, 472, 806, 856, 1167
SchI GAGTC 2 cut(s) 716, 785
ScrFI CCNGG 4 cut(s) 320, 561, 803, 810
SduI GDGCHC 1 cut(s) 201
SfaNI GCATC 3 cut(s) 15, 79, 292
SfcI CTRYAG 3 cut(s) 638, 693, 1077
SinI GGWCC 4 cut(s) 418, 472, 806, 856
SmiMI CAYNNNNRTG 2 cut(s) 866, 1351
SphI GCATGC 1 cut(s) 710
SseBI AGGCCT 1 cut(s) 875
SsiI CCGC 2 cut(s) 353, 475
SspMI CTAG 3 cut(s) 311, 380, 903
SstI GAGCTC 1 cut(s) 201
StuI AGGCCT 1 cut(s) 875
StyD4I CCNGG 4 cut(s) 318, 559, 801, 808
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 3 cut(s) 970, 1138, 1306
TaqI TCGA 3 cut(s) 504, 1106, 1311
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 4 cut(s) 24, 564, 813, 1039
Tru1I TTAA 4 cut(s) 120, 411, 462, 924
Tru9I TTAA 4 cut(s) 120, 411, 462, 924
TscAI CASTG 4 cut(s) 219, 370, 697, 1234
TseI GCWGC 5 cut(s) 188, 307, 393, 1076, 1079
TspDTI ATGAA 8 cut(s) 16, 41, 87, 134, 810, 1068, 1086, 1131
TspRI CASTG 4 cut(s) 219, 370, 697, 1234
VpaK11BI GGWCC 4 cut(s) 418, 472, 806, 856
XapI RAATTY 3 cut(s) 140, 275, 493
XbaI TCTAGA 1 cut(s) 902
XceI RCATGY 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 717
XmnI GAANNNNTTC 1 cut(s) 817
XspI CTAG 3 cut(s) 311, 380, 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.