FvH4_5g21440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016347
Physical Location & Seq
Reverse (-)
12911930 .. 12913198
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21440.t1

Sequence Viewer

Length: 1269 bp
ATGCGAGGAAAACAAAGTAGGAAAACAAAGTACAAGTTAGTCTGCAGTTCCAATCCCCAAACCCAATCAAGACTTGAAAACCCTCCTGCAGCTGATCACACAGAGATGAAGATCTCCCTTCGTCTCCAAGAGGACCAAGACGCCCAGCACTACCGCAACCCACTTGTAAGAGCCAAAATCCCCATCACCATTTTCAACCAACCTTTCACTTCTTCAATCGCCACCGTCCCTTCCTCTACCACCTCCTCTCACCTCTCCTTTTCTCTCTCCTCAGCCTTCTCCTCCGGCCCTTCTCTCAAACTCTCTTACTCTCACACCGACCCATCTCCCTTCTCTCTCTCCCTCAAGTCTGGCTTGGGCCTCTTAGGCTCCCCAAACCACTCCCCTCTTGTTTTCACTGCCCACTTCTCTCTCTCCCACACAAACCCCACTTTCCTTCTCCACGTCAAGCCCCAATTCGGAAACTTTTCTCTGAAAAAGACCACCTTTTCGGACCCTGAGGTTTCTGGGTCCTGCTCAAATGGTGTTTTGGATTCTGGGTCTGGCTCAGATGGTGGTTTTGTTGATGGGTTTGTACAAGGGTCGTCGTCTGCTTGGCAGGAGCTGAGTTTGGAGCCTCGTGGGGGTAAAGATGTAAATAGTGGAGTTGGGTCTGTGGAGGAGAGGCAGTTGGGTTGGAAGAATGGTGGGAAAGATGGGTTTTTGTCTGGGATTGCTGTGATGGCCAGGACTGTTTTGCCTGTGACCAAAGGAGTTATGGTGAATATGCGGTGGGGTGTGAACTTTCCGGCCAAGTTGGGGTCCAAGCTGCCGTATTTGACAGTTAATAAGATTGCTGTTGAGACAGTTGAAGAGGTGAAGGGACAGGAGACGGAGAAGAAGAATGGTGGGAGTAATGTGGGGGATTTGGAATTGTTGAAGGGGATGTGTGTTTGGATGAGGAGGGATTTGGAGGTTTTGGAGAAGGAGAATAGGGACATGAAGCAGAGATTGGAGGATATGAAGTTGGGAGTTTCCGGGAGGCAGTTTTGTGGGGAAAGTGATGGTTCAAGGAGGAGAGTGGTGCCACCATTGGGTGAGAGTTTGGGTGAGTTTGAAAGGTGGAGGAACAAGAAAGGTGTCAGAGAAGAGAATGTGCAAACAGAGTCGCAATCGCCAGTGAAAAGTTTCAGGGAAGAGAGTTCTCGGGTGGAGGCGAAGAAGTCCACCAATCAGGCAAGTGATTTGGAATCTGAATTGGAGAGAGCTATAAAGGCTGCTACTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

423

Amino Acids

46.12

Weight (kDa)

9.18

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016347)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21440
malus_domestica MD06G1029200.v1.1
prunus_persica Prupe.5G042700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0208871
rosa_laevigata RLG00000003188
rosa_multiflora Rmu_sc0000532.1_g000005 Rmu_sc0001482.1_g000003 Rmu_sc0016359.1_g000001
rosa_roxburghii Rroxscaffold_3G00250090
rosa_rugosa Rorug07G0107000
rosa_samantha Rh7AG242000 Rh7BG236800 Rh7CG256900 Rh7DG247900
rosa_wichuraiana Rw7G020490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1063
AciI CCGC 2 cut(s) 154, 769
AcoI YGGCCR 2 cut(s) 723, 789
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 2 cut(s) 32, 576
AfiI CCNNNNNNNGG 4 cut(s) 458, 623, 798, 1073
AgsI TTSAA 7 cut(s) 77, 196, 216, 851, 919, 1050, 1097
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 725
AjuI GAANNNNNNNTTGG 2 cut(s) 974, 1006
AluBI AGCT 4 cut(s) 92, 604, 808, 1247
AluI AGCT 4 cut(s) 92, 604, 808, 1247
Alw26I GTCTC 3 cut(s) 128, 836, 863
AlwNI CAGNNNCTG 1 cut(s) 604
Ama87I CYCGRG 1 cut(s) 1185
AoxI GGCC 4 cut(s) 286, 358, 723, 789
ApeKI GCWGC 3 cut(s) 89, 808, 1256
ArsI GACNNNNNNTTYG 2 cut(s) 472, 504
Asp700I GAANNNNTTC 2 cut(s) 466, 1166
AspS9I GGNCC 6 cut(s) 133, 287, 358, 493, 510, 801
AsuC2I CCSGG 1 cut(s) 1018
AsuHPI GGTGA 6 cut(s) 178, 242, 772, 868, 1088, 1100
AvaI CYCGRG 1 cut(s) 1185
AvaII GGWCC 4 cut(s) 133, 493, 510, 801
AxyI CCTNAGG 1 cut(s) 498
BalI TGGCCA 1 cut(s) 725
BanI GGYRCC 1 cut(s) 1063
BauI CACGAG 1 cut(s) 618
BbvCI CCTCAGC 1 cut(s) 271
BbvI GCAGC 3 cut(s) 101, 795, 1243
BccI CCATC 7 cut(s) 191, 331, 545, 560, 689, 715, 1037
BceAI ACGGC 1 cut(s) 796
BciT130I CCWGG 1 cut(s) 727
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 1018
BcoDI GTCTC 3 cut(s) 128, 836, 863
BfaI CTAG 1 cut(s) 1267
BfmI CTRYAG 2 cut(s) 43, 87
BglI GCCNNNNNGGC 1 cut(s) 366
BglII AGATCT 1 cut(s) 111
BisI GCNGC 3 cut(s) 90, 809, 1257
BlsI GCNGC 3 cut(s) 91, 810, 1258
Bme1390I CCNGG 2 cut(s) 727, 1018
Bme18I GGWCC 4 cut(s) 133, 493, 510, 801
BmeT110I CYCGRG 1 cut(s) 1185
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 6 cut(s) 133, 287, 358, 493, 510, 801
BmiI GGNNCC 6 cut(s) 370, 495, 511, 615, 802, 1065
BmrFI CCNGG 2 cut(s) 727, 1018
Bpu10I CCTNAGC 1 cut(s) 271
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 1018
BsaBI GATNNNNATC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 141
BsaXI ACNNNNNCTCC 4 cut(s) 653, 683, 744, 774
Bsc4I CCNNNNNNNGG 4 cut(s) 458, 623, 798, 1073
Bse1I ACTGG 1 cut(s) 1157
Bse21I CCTNAGG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 110
BseBI CCWGG 1 cut(s) 727
BseGI GGATG 2 cut(s) 930, 942
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 4 cut(s) 458, 623, 798, 1073
BseMII CTCAG 4 cut(s) 285, 489, 561, 596
BseNI ACTGG 1 cut(s) 1157
BseRI GAGGAG 6 cut(s) 235, 259, 271, 674, 955, 1069
BseXI GCAGC 3 cut(s) 101, 795, 1243
BseYI CCCAGC 1 cut(s) 144
BshFI GGCC 4 cut(s) 288, 360, 725, 791
BshNI GGYRCC 1 cut(s) 1063
BsiHKCI CYCGRG 1 cut(s) 1185
BsiSI CCGG 3 cut(s) 285, 788, 1017
BslFI GGGAC 3 cut(s) 212, 876, 989
BslI CCNNNNNNNGG 4 cut(s) 458, 623, 798, 1073
BsmAI GTCTC 3 cut(s) 128, 836, 863
BsmBI CGTCTC 2 cut(s) 128, 863
BsmFI GGGAC 3 cut(s) 212, 876, 989
BsnI GGCC 4 cut(s) 288, 360, 725, 791
BsoBI CYCGRG 1 cut(s) 1185
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 2 cut(s) 94, 111
BspACI CCGC 2 cut(s) 154, 769
BspANI GGCC 4 cut(s) 288, 360, 725, 791
BspCNI CTCAG 4 cut(s) 284, 490, 560, 597
BspLI GGNNCC 6 cut(s) 370, 495, 511, 615, 802, 1065
BspMAI CTGCAG 2 cut(s) 47, 91
BspT107I GGYRCC 1 cut(s) 1063
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 1 cut(s) 1157
BssMI GATC 2 cut(s) 94, 111
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 618
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 727
Bst4CI ACNGT 4 cut(s) 226, 733, 823, 847
Bst6I CTCTTC 3 cut(s) 846, 1122, 1170
BstACI GRCGYC 1 cut(s) 141
BstAUI TGTACA 1 cut(s) 574
BstDEI CTNAG 5 cut(s) 271, 364, 498, 547, 605
BstF5I GGATG 2 cut(s) 930, 942
BstKTI GATC 2 cut(s) 97, 114
BstMAI GTCTC 3 cut(s) 128, 836, 863
BstMBI GATC 2 cut(s) 94, 111
BstMWI GCNNNNNNNGC 4 cut(s) 366, 722, 1253, 1262
BstNI CCWGG 1 cut(s) 727
BstSCI CCNGG 2 cut(s) 725, 1016
BstSFI CTRYAG 2 cut(s) 43, 87
BstV1I GCAGC 3 cut(s) 101, 795, 1243
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
Bsu36I CCTNAGG 1 cut(s) 498
BsuRI GGCC 4 cut(s) 288, 360, 725, 791
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 2 cut(s) 930, 942
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 396, 1164
CaiI CAGNNNCTG 1 cut(s) 604
Cfr13I GGNCC 6 cut(s) 133, 287, 358, 493, 510, 801
CseI GACGC 1 cut(s) 149
Csp6I GTAC 2 cut(s) 31, 575
CviAII CATG 1 cut(s) 979
CviQI GTAC 2 cut(s) 31, 575
DdeI CTNAG 5 cut(s) 271, 364, 498, 547, 605
DpnI GATC 2 cut(s) 96, 113
DpnII GATC 2 cut(s) 94, 111
EaeI YGGCCR 2 cut(s) 723, 789
Eam1104I CTCTTC 3 cut(s) 846, 1122, 1170
EarI CTCTTC 3 cut(s) 846, 1122, 1170
Eco47I GGWCC 4 cut(s) 133, 493, 510, 801
Eco81I CCTNAGG 1 cut(s) 498
Eco88I CYCGRG 1 cut(s) 1185
EcoO109I RGGNCCY 1 cut(s) 510
EcoRII CCWGG 1 cut(s) 725
Esp3I CGTCTC 2 cut(s) 128, 863
FaeI CATG 1 cut(s) 982
FaiI YATR 5 cut(s) 758, 767, 980, 1001, 1250
FalI AAGNNNNNCTT 4 cut(s) 338, 370, 470, 502
FaqI GGGAC 3 cut(s) 212, 876, 989
FatI CATG 1 cut(s) 978
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 90, 809, 1257
FokI GGATG 2 cut(s) 937, 949
Fsp4HI GCNGC 3 cut(s) 90, 809, 1257
FspBI CTAG 1 cut(s) 1267
GluI GCNGC 3 cut(s) 90, 809, 1257
GsaI CCCAGC 1 cut(s) 148
HaeIII GGCC 4 cut(s) 288, 360, 725, 791
HapII CCGG 3 cut(s) 285, 788, 1017
HgaI GACGC 1 cut(s) 149
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 1 cut(s) 982
HinfI GANTC 3 cut(s) 533, 1145, 1229
HpaII CCGG 3 cut(s) 285, 788, 1017
HphI GGTGA 6 cut(s) 178, 242, 772, 868, 1088, 1100
Hpy166II GTNNAC 2 cut(s) 781, 1206
Hpy188I TCNGA 6 cut(s) 461, 474, 493, 550, 1124, 1234
Hpy188III TCNNGA 1 cut(s) 69
Hpy8I GTNNAC 2 cut(s) 781, 1206
Hpy99I CGWCG 1 cut(s) 589
HpyAV CCTTC 9 cut(s) 128, 240, 286, 300, 340, 446, 853, 913, 958
HpyCH4III ACNGT 4 cut(s) 226, 733, 823, 847
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 3 cut(s) 45, 89, 1138
HpyF10VI GCNNNNNNNGC 4 cut(s) 366, 722, 1253, 1262
HpyF3I CTNAG 5 cut(s) 271, 364, 498, 547, 605
HpySE526I ACGT 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 1 cut(s) 982
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 2 cut(s) 94, 111
LmnI GCTCC 3 cut(s) 374, 601, 613
Lsp1109I GCAGC 3 cut(s) 101, 795, 1243
MaeI CTAG 1 cut(s) 1267
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 1 cut(s) 742
MalI GATC 2 cut(s) 96, 113
MboI GATC 2 cut(s) 94, 111
MboII GAAGA 9 cut(s) 121, 204, 691, 863, 889, 892, 1139, 1187, 1210
MflI RGATCY 1 cut(s) 111
MlsI TGGCCA 1 cut(s) 725
MluCI AATT 3 cut(s) 455, 911, 1235
MluNI TGGCCA 1 cut(s) 725
MlyI GAGTC 1 cut(s) 1154
MmeI TCCRAC 1 cut(s) 656
Mox20I TGGCCA 1 cut(s) 725
MroXI GAANNNNTTC 2 cut(s) 466, 1166
MscI TGGCCA 1 cut(s) 725
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 1 cut(s) 104
Msp20I TGGCCA 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 3 cut(s) 285, 788, 1017
MspR9I CCNGG 2 cut(s) 727, 1018
MvaI CCWGG 1 cut(s) 727
MwoI GCNNNNNNNGC 4 cut(s) 366, 722, 1253, 1262
NciI CCSGG 1 cut(s) 1018
NdeII GATC 2 cut(s) 94, 111
NlaIII CATG 1 cut(s) 982
NlaIV GGNNCC 6 cut(s) 370, 495, 511, 615, 802, 1065
NmuCI GTSAC 1 cut(s) 742
PdmI GAANNNNTTC 2 cut(s) 466, 1166
PfeI GAWTC 2 cut(s) 533, 1229
PfoI TCCNGGA 1 cut(s) 1016
PkrI GCNGC 3 cut(s) 91, 810, 1258
PleI GAGTC 1 cut(s) 1153
PpsI GAGTC 1 cut(s) 1153
PpuMI RGGWCCY 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 725
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 1 cut(s) 725
PspN4I GGNNCC 6 cut(s) 370, 495, 511, 615, 802, 1065
PspPI GGNCC 6 cut(s) 133, 287, 358, 493, 510, 801
PspPPI RGGWCCY 1 cut(s) 510
PstI CTGCAG 2 cut(s) 47, 91
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 2 cut(s) 32, 576
RsaNI GTAC 2 cut(s) 31, 575
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 3 cut(s) 90, 809, 1257
Sau3AI GATC 2 cut(s) 94, 111
Sau96I GGNCC 6 cut(s) 133, 287, 358, 493, 510, 801
SchI GAGTC 1 cut(s) 1154
ScrFI CCNGG 2 cut(s) 727, 1018
SfcI CTRYAG 2 cut(s) 43, 87
SinI GGWCC 4 cut(s) 133, 493, 510, 801
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 3 cut(s) 455, 911, 1235
SsiI CCGC 2 cut(s) 154, 769
SspMI CTAG 1 cut(s) 1267
StyD4I CCNGG 2 cut(s) 725, 1016
TaaI ACNGT 4 cut(s) 226, 733, 823, 847
TaiI ACGT 1 cut(s) 447
TasI AATT 3 cut(s) 455, 911, 1235
TatI WGTACW 2 cut(s) 30, 574
TfiI GAWTC 2 cut(s) 533, 1229
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 2 cut(s) 403, 1164
TseFI GTSAC 1 cut(s) 742
TseI GCWGC 3 cut(s) 89, 808, 1256
Tsp45I GTSAC 1 cut(s) 742
TspDTI ATGAA 3 cut(s) 122, 995, 1016
TspGWI ACGGA 1 cut(s) 887
TspRI CASTG 2 cut(s) 403, 1164
VpaK11BI GGWCC 4 cut(s) 133, 493, 510, 801
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XmnI GAANNNNTTC 2 cut(s) 466, 1166
XspI CTAG 1 cut(s) 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.