Belongs to the pyruvate kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 122 |
| AccB1I | GGYRCC | 1 cut(s) | 122 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 479 |
| AciI | CCGC | 2 cut(s) | 27, 192 |
| AclWI | GGATC | 1 cut(s) | 183 |
| AcsI | RAATTY | 1 cut(s) | 500 |
| AfaI | GTAC | 2 cut(s) | 124, 152 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 113, 274, 479 |
| AgsI | TTSAA | 3 cut(s) | 61, 298, 557 |
| AjnI | CCWGG | 3 cut(s) | 106, 267, 520 |
| AluBI | AGCT | 4 cut(s) | 227, 367, 489, 671 |
| AluI | AGCT | 4 cut(s) | 227, 367, 489, 671 |
| Alw21I | GWGCWC | 3 cut(s) | 68, 414, 717 |
| AlwI | GGATC | 1 cut(s) | 183 |
| ApeKI | GCWGC | 2 cut(s) | 200, 575 |
| ApoI | RAATTY | 1 cut(s) | 500 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 320, 603 |
| Asp718I | GGTACC | 1 cut(s) | 122 |
| AspLEI | GCGC | 1 cut(s) | 87 |
| AspS9I | GGNCC | 2 cut(s) | 101, 352 |
| AsuHPI | GGTGA | 1 cut(s) | 461 |
| AvaII | GGWCC | 2 cut(s) | 101, 352 |
| BaeGI | GKGCMC | 1 cut(s) | 24 |
| BanI | GGYRCC | 1 cut(s) | 122 |
| Bbv12I | GWGCWC | 3 cut(s) | 68, 414, 717 |
| BbvI | GCAGC | 2 cut(s) | 187, 587 |
| BccI | CCATC | 2 cut(s) | 326, 481 |
| BceAI | ACGGC | 1 cut(s) | 563 |
| BciT130I | CCWGG | 3 cut(s) | 108, 269, 522 |
| BfaI | CTAG | 1 cut(s) | 368 |
| BfmI | CTRYAG | 1 cut(s) | 573 |
| BfoI | RGCGCY | 1 cut(s) | 88 |
| BisI | GCNGC | 2 cut(s) | 201, 576 |
| BlsI | GCNGC | 2 cut(s) | 202, 577 |
| BmcAI | AGTACT | 1 cut(s) | 152 |
| Bme1390I | CCNGG | 3 cut(s) | 108, 269, 522 |
| Bme18I | GGWCC | 2 cut(s) | 101, 352 |
| BmgT120I | GGNCC | 2 cut(s) | 101, 352 |
| BmiI | GGNNCC | 3 cut(s) | 102, 124, 519 |
| BmrFI | CCNGG | 3 cut(s) | 108, 269, 522 |
| BmsI | GCATC | 2 cut(s) | 288, 366 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 534, 566 |
| BpmI | CTGGAG | 1 cut(s) | 561 |
| Bpu10I | CCTNAGC | 2 cut(s) | 112, 228 |
| BpuEI | CTTGAG | 1 cut(s) | 569 |
| BsaJI | CCNNGG | 1 cut(s) | 513 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 240, 270, 384, 414 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 113, 274, 479 |
| Bse118I | RCCGGY | 1 cut(s) | 173 |
| Bse1I | ACTGG | 3 cut(s) | 477, 544, 623 |
| BseBI | CCWGG | 3 cut(s) | 108, 269, 522 |
| BseDI | CCNNGG | 1 cut(s) | 513 |
| BseGI | GGATG | 2 cut(s) | 473, 571 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 113, 274, 479 |
| BseMII | CTCAG | 2 cut(s) | 126, 663 |
| BseNI | ACTGG | 3 cut(s) | 477, 544, 623 |
| BseRI | GAGGAG | 1 cut(s) | 201 |
| BseSI | GKGCMC | 1 cut(s) | 24 |
| BseXI | GCAGC | 2 cut(s) | 187, 587 |
| BshNI | GGYRCC | 1 cut(s) | 122 |
| BsiHKAI | GWGCWC | 3 cut(s) | 68, 414, 717 |
| BsiSI | CCGG | 1 cut(s) | 174 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 113, 274, 479 |
| Bsp1286I | GDGCHC | 4 cut(s) | 24, 68, 414, 717 |
| Bsp143I | GATC | 2 cut(s) | 31, 188 |
| BspACI | CCGC | 2 cut(s) | 27, 192 |
| BspCNI | CTCAG | 2 cut(s) | 125, 664 |
| BspLI | GGNNCC | 3 cut(s) | 102, 124, 519 |
| BspMAI | CTGCAG | 1 cut(s) | 577 |
| BspPI | GGATC | 1 cut(s) | 183 |
| BspT107I | GGYRCC | 1 cut(s) | 122 |
| BsrFI | RCCGGY | 1 cut(s) | 173 |
| BsrI | ACTGG | 3 cut(s) | 477, 544, 623 |
| BssAI | RCCGGY | 1 cut(s) | 173 |
| BssECI | CCNNGG | 1 cut(s) | 513 |
| BssMI | GATC | 2 cut(s) | 31, 188 |
| BssT1I | CCWWGG | 1 cut(s) | 513 |
| Bst2UI | CCWGG | 3 cut(s) | 108, 269, 522 |
| Bst4CI | ACNGT | 2 cut(s) | 349, 697 |
| Bst6I | CTCTTC | 1 cut(s) | 438 |
| BstDEI | CTNAG | 4 cut(s) | 112, 204, 228, 672 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 111 |
| BstF5I | GGATG | 2 cut(s) | 473, 571 |
| BstH2I | RGCGCY | 1 cut(s) | 88 |
| BstHHI | GCGC | 1 cut(s) | 87 |
| BstKTI | GATC | 2 cut(s) | 34, 191 |
| BstMBI | GATC | 2 cut(s) | 31, 188 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 82, 200, 233 |
| BstNI | CCWGG | 3 cut(s) | 108, 269, 522 |
| BstSCI | CCNGG | 3 cut(s) | 106, 267, 520 |
| BstSFI | CTRYAG | 1 cut(s) | 573 |
| BstSLI | GKGCMC | 1 cut(s) | 24 |
| BstV1I | GCAGC | 2 cut(s) | 187, 587 |
| BstX2I | RGATCY | 1 cut(s) | 188 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 389 |
| BstYI | RGATCY | 1 cut(s) | 188 |
| BtsCI | GGATG | 2 cut(s) | 473, 571 |
| BtsIMutI | CAGTG | 3 cut(s) | 46, 484, 693 |
| CfoI | GCGC | 1 cut(s) | 87 |
| Cfr10I | RCCGGY | 1 cut(s) | 173 |
| Cfr13I | GGNCC | 2 cut(s) | 101, 352 |
| CsiI | ACCWGGT | 1 cut(s) | 267 |
| Csp6I | GTAC | 2 cut(s) | 123, 151 |
| CviQI | GTAC | 2 cut(s) | 123, 151 |
| DdeI | CTNAG | 4 cut(s) | 112, 204, 228, 672 |
| DpnI | GATC | 2 cut(s) | 33, 190 |
| DpnII | GATC | 2 cut(s) | 31, 188 |
| Eam1104I | CTCTTC | 1 cut(s) | 438 |
| EarI | CTCTTC | 1 cut(s) | 438 |
| EciI | GGCGGA | 1 cut(s) | 16 |
| Eco130I | CCWWGG | 1 cut(s) | 513 |
| Eco47I | GGWCC | 2 cut(s) | 101, 352 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 111 |
| EcoRII | CCWGG | 3 cut(s) | 106, 267, 520 |
| EcoT14I | CCWWGG | 1 cut(s) | 513 |
| ErhI | CCWWGG | 1 cut(s) | 513 |
| FaiI | YATR | 2 cut(s) | 182, 239 |
| Fnu4HI | GCNGC | 2 cut(s) | 201, 576 |
| FokI | GGATG | 2 cut(s) | 460, 578 |
| Fsp4HI | GCNGC | 2 cut(s) | 201, 576 |
| FspBI | CTAG | 1 cut(s) | 368 |
| GlaI | GCGC | 1 cut(s) | 86 |
| GluI | GCNGC | 2 cut(s) | 201, 576 |
| GsuI | CTGGAG | 1 cut(s) | 561 |
| HaeII | RGCGCY | 1 cut(s) | 88 |
| HapII | CCGG | 1 cut(s) | 174 |
| HhaI | GCGC | 1 cut(s) | 87 |
| Hin6I | GCGC | 1 cut(s) | 85 |
| HinP1I | GCGC | 1 cut(s) | 85 |
| HinfI | GANTC | 3 cut(s) | 208, 431, 466 |
| HpaII | CCGG | 1 cut(s) | 174 |
| HphI | GGTGA | 1 cut(s) | 461 |
| Hpy166II | GTNNAC | 1 cut(s) | 104 |
| Hpy188I | TCNGA | 4 cut(s) | 262, 342, 684, 708 |
| Hpy188III | TCNNGA | 2 cut(s) | 114, 329 |
| Hpy8I | GTNNAC | 1 cut(s) | 104 |
| Hpy99I | CGWCG | 2 cut(s) | 57, 724 |
| HpyAV | CCTTC | 2 cut(s) | 238, 315 |
| HpyCH4III | ACNGT | 2 cut(s) | 349, 697 |
| HpyCH4IV | ACGT | 1 cut(s) | 559 |
| HpyCH4V | TGCA | 3 cut(s) | 422, 506, 575 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 82, 200, 233 |
| HpyF3I | CTNAG | 4 cut(s) | 112, 204, 228, 672 |
| HpySE526I | ACGT | 1 cut(s) | 559 |
| HspAI | GCGC | 1 cut(s) | 85 |
| KpnI | GGTACC | 1 cut(s) | 126 |
| Kzo9I | GATC | 2 cut(s) | 31, 188 |
| LmnI | GCTCC | 2 cut(s) | 71, 384 |
| Lsp1109I | GCAGC | 2 cut(s) | 187, 587 |
| LweI | GCATC | 2 cut(s) | 288, 366 |
| MabI | ACCWGGT | 1 cut(s) | 267 |
| MaeI | CTAG | 1 cut(s) | 368 |
| MaeII | ACGT | 1 cut(s) | 559 |
| MaeIII | GTNAC | 2 cut(s) | 535, 691 |
| MalI | GATC | 2 cut(s) | 33, 190 |
| MboI | GATC | 2 cut(s) | 31, 188 |
| MboII | GAAGA | 4 cut(s) | 26, 310, 455, 656 |
| MfeI | CAATTG | 1 cut(s) | 423 |
| MflI | RGATCY | 1 cut(s) | 188 |
| MhlI | GDGCHC | 4 cut(s) | 24, 68, 414, 717 |
| MluCI | AATT | 2 cut(s) | 423, 500 |
| MlyI | GAGTC | 2 cut(s) | 202, 425 |
| MroXI | GAANNNNTTC | 2 cut(s) | 320, 603 |
| MseI | TTAA | 3 cut(s) | 141, 222, 396 |
| MslI | CAYNNNNRTG | 1 cut(s) | 95 |
| MspA1I | CMGCKG | 2 cut(s) | 194, 671 |
| MspI | CCGG | 1 cut(s) | 174 |
| MspR9I | CCNGG | 3 cut(s) | 108, 269, 522 |
| MunI | CAATTG | 1 cut(s) | 423 |
| MvaI | CCWGG | 3 cut(s) | 108, 269, 522 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 82, 200, 233 |
| NdeII | GATC | 2 cut(s) | 31, 188 |
| NlaIV | GGNNCC | 3 cut(s) | 102, 124, 519 |
| NmuCI | GTSAC | 1 cut(s) | 691 |
| PdmI | GAANNNNTTC | 2 cut(s) | 320, 603 |
| PfeI | GAWTC | 1 cut(s) | 466 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 479 |
| PkrI | GCNGC | 2 cut(s) | 202, 577 |
| PleI | GAGTC | 2 cut(s) | 202, 425 |
| PpsI | GAGTC | 2 cut(s) | 202, 425 |
| Psp6I | CCWGG | 3 cut(s) | 106, 267, 520 |
| PspGI | CCWGG | 3 cut(s) | 106, 267, 520 |
| PspN4I | GGNNCC | 3 cut(s) | 102, 124, 519 |
| PspPI | GGNCC | 2 cut(s) | 101, 352 |
| PstI | CTGCAG | 1 cut(s) | 577 |
| PsuI | RGATCY | 1 cut(s) | 188 |
| PvuII | CAGCTG | 1 cut(s) | 671 |
| RsaI | GTAC | 2 cut(s) | 124, 152 |
| RsaNI | GTAC | 2 cut(s) | 123, 151 |
| RseI | CAYNNNNRTG | 1 cut(s) | 95 |
| SaqAI | TTAA | 3 cut(s) | 141, 222, 396 |
| SatI | GCNGC | 2 cut(s) | 201, 576 |
| Sau3AI | GATC | 2 cut(s) | 31, 188 |
| Sau96I | GGNCC | 2 cut(s) | 101, 352 |
| ScaI | AGTACT | 1 cut(s) | 152 |
| SchI | GAGTC | 2 cut(s) | 202, 425 |
| ScrFI | CCNGG | 3 cut(s) | 108, 269, 522 |
| SduI | GDGCHC | 4 cut(s) | 24, 68, 414, 717 |
| SexAI | ACCWGGT | 1 cut(s) | 267 |
| SfaNI | GCATC | 2 cut(s) | 288, 366 |
| SfcI | CTRYAG | 1 cut(s) | 573 |
| SinI | GGWCC | 2 cut(s) | 101, 352 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 95 |
| SmlI | CTYRAG | 1 cut(s) | 584 |
| SmoI | CTYRAG | 1 cut(s) | 584 |
| Sse9I | AATT | 2 cut(s) | 423, 500 |
| SsiI | CCGC | 2 cut(s) | 27, 192 |
| SspMI | CTAG | 1 cut(s) | 368 |
| StyD4I | CCNGG | 3 cut(s) | 106, 267, 520 |
| StyI | CCWWGG | 1 cut(s) | 513 |
| TaaI | ACNGT | 2 cut(s) | 349, 697 |
| TaiI | ACGT | 1 cut(s) | 562 |
| TaqI | TCGA | 1 cut(s) | 5 |
| TasI | AATT | 2 cut(s) | 423, 500 |
| TatI | WGTACW | 1 cut(s) | 150 |
| TfiI | GAWTC | 1 cut(s) | 466 |
| Tru1I | TTAA | 3 cut(s) | 141, 222, 396 |
| Tru9I | TTAA | 3 cut(s) | 141, 222, 396 |
| TscAI | CASTG | 3 cut(s) | 46, 484, 700 |
| TseFI | GTSAC | 1 cut(s) | 691 |
| TseI | GCWGC | 2 cut(s) | 200, 575 |
| Tsp45I | GTSAC | 1 cut(s) | 691 |
| TspDTI | ATGAA | 2 cut(s) | 303, 313 |
| TspRI | CASTG | 3 cut(s) | 46, 484, 700 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 479 |
| VpaK11BI | GGWCC | 2 cut(s) | 101, 352 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 111 |
| XapI | RAATTY | 1 cut(s) | 500 |
| XmnI | GAANNNNTTC | 2 cut(s) | 320, 603 |
| XspI | CTAG | 1 cut(s) | 368 |
| ZrmI | AGTACT | 1 cut(s) | 152 |