pycom06g02360

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001739
Physical Location & Seq
Forward (+)
2828671 .. 2835070
6400 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g02360.11

Sequence Viewer

Length: 1731 bp
ATGGCGACTCTTAATGTTCCCGCGAGAATGTCAGTATTGAAACCTGGCAAGACTAAGGCCGCTGATCGATTGGTTTCCTCCAAGTGCCTGACTGGTTCCGTTGGGTTCGACTGCGGACCCAGAAGAAAATATGCCCACAACCACAAGAACCACAAGCAATTTTTTACTGTTAAATCAATTGGGATCACCCAGGACAGCAGCAGAGGCCATGTCAACGGTCCTCTTGGTGGCGTCGGGAATCTGGACTCAACTTTTGAACTTCAGTCTGATGAGTATGTTGTGGGCCAGCCGAAGGTATTTTCAGATGGTCAAAGAAAAACTAAGATAGTATGTACGATTGGTCCCTCGACAAGCTCACGTGAAATGATATGGAAACTGGCGGAAACTGGAATGAACGTCGCACGTTTGAATATGTCACATGGAGACCATGCATCTCATCAGAAAACCATTGAACTTGTCAAAGAATACAATTCTCAATTTGAAGACAAGGTTGTAGCAATAATGCTGGACACCAAGGGTCCTGAGGTTAGGAGTGGAGATGTACCACAGCCGATTCTACTTAAAGAAGGACAAGAGTTTAACTTTACAATTAAAAGGGGAGTTAGCACTGCAGACACTGTTAGCGTAAACTATGATGGCTTTGTGAATGACGTGGAGGTTGGTGACATTATTCTAGTTGATGGTGGAATGATGTCTTTAGCTGTAAAGTCAAAGACAAAAGATGTGGTTAAATGTGTGGTAGTTGATGGTGGAGAACTAAAATCAAGGCGCCATCTAAACGTGCGTGGAAAAAGTGCAACTCTGCCTTCAATAACAGAAAAGGACTGGGAAGATATCAAGTTTGGAGTGGACAATGGAGTTGATTTCTATGCTGTATCATTTGTAAAAAATGCTGCAGTGGTACACGAGTTGAAAGATTATCTCAAAGGTTGTAATGCAGATATTCATGTTATTGTAAAAATAGAAAGCGCGGACTCTATACCAAATCTTCATTCAATAATTTCTGCATCTGACGGGGCGATGATTGCTCGTGGAGACCTTGGAGCTGAGCTGCCAATTGAGGAAGTTCCTCTGTTGCAGGAGGACATCATTAGAAGGTGTCGCAATGTGCAGAAACCAGTGATTGTAGCAACAAACATGCTGGAGAGCATGATTGGTCATCCTACGCCGACCAGGGCAGAAGTCTCTGACATTGCAATTGCAGTAAGAGCAGGTGCTGATGCAACCATGCTTTCTGGAGAAACTGCCCATGGAAAGTATCCGTTGAAGGCTGTCAAAGTGATGCATACTGTAGCATTAAGGACTGAGTCAAGTCTACCAATTAGCACTATTCCTCCAATACAACTGAGGACCTTTAAGAAACATATGGGGGAAATGTTTGCTTTCCATTCCACAACTATAGCAAACAGTCTCAATACACCCATCATTGTTTTCACAAGGACAGGTTCCATGGCTGTGCTTTTAAGCCATTATCGTCCTTGCTCAACGATCTTTGCCTTCACAAACGAAGAGAGAATTAAGCAGAGATTGGTTCTTTATCACGGTGTCCGCCCCATATATATGCGGTTCTCAAATGATGCGGAGGAGACTTTCTCAAGAGCAATAAAGCTACTAGTGGCTAAGGATCTTTTGAAAGAGGGACAGTTTGTAACTCTCGTCCAAAGCGGTGCACAACCAATCTGGCGTAAAGAGTCTTCTCACCATATCCAAGTTCGTAAAGTTGAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

577

Amino Acids

63.3

Weight (kDa)

8.98

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1202
Acc36I ACCTGC 1 cut(s) 1202
AccB1I GGYRCC 1 cut(s) 768
AccI GTMKAC 1 cut(s) 1315
AccII CGCG 2 cut(s) 23, 971
AciI CCGC 9 cut(s) 21, 60, 114, 380, 971, 1549, 1564, 1580, 1665
AclWI GGATC 2 cut(s) 191, 1632
AcuI CTGAAG 1 cut(s) 245
AcvI CACGTG 1 cut(s) 359
AcyI GRCGYC 2 cut(s) 231, 769
AfaI GTAC 3 cut(s) 334, 543, 903
AfiI CCNNNNNNNGG 2 cut(s) 227, 292
AhlI ACTAGT 1 cut(s) 1612
AjiI CACGTC 1 cut(s) 652
AjnI CCWGG 3 cut(s) 43, 189, 1172
AjuI GAANNNNNNNTTGG 2 cut(s) 976, 1008
AluBI AGCT 5 cut(s) 354, 701, 1046, 1051, 1609
AluI AGCT 5 cut(s) 354, 701, 1046, 1051, 1609
Alw21I GWGCWC 1 cut(s) 1672
Alw26I GTCTC 5 cut(s) 417, 1029, 1189, 1415, 1580
Alw44I GTGCAC 1 cut(s) 1668
AlwI GGATC 2 cut(s) 191, 1632
AlwNI CAGNNNCTG 2 cut(s) 617, 1217
AoxI GGCC 3 cut(s) 57, 205, 283
ApaLI GTGCAC 1 cut(s) 1668
ApeKI GCWGC 3 cut(s) 198, 893, 1051
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AspLEI GCGC 2 cut(s) 771, 971
AspS9I GGNCC 6 cut(s) 116, 218, 283, 341, 518, 1350
AsuHPI GGTGA 3 cut(s) 178, 674, 1691
AvaII GGWCC 5 cut(s) 116, 218, 341, 518, 1350
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 1672
BaeI ACNNNNGTAYC 2 cut(s) 893, 926
BanI GGYRCC 1 cut(s) 768
BarI GAAGNNNNNNTAC 2 cut(s) 1678, 1710
BauI CACGAG 2 cut(s) 905, 1029
BbrPI CACGTG 1 cut(s) 359
BbsI GAAGAC 2 cut(s) 489, 1686
Bbv12I GWGCWC 1 cut(s) 1672
BbvI GCAGC 3 cut(s) 210, 880, 1038
BccI CCATC 6 cut(s) 299, 629, 674, 740, 780, 1430
BciT130I CCWGG 3 cut(s) 45, 191, 1174
BciVI GTATCC 1 cut(s) 1269
BcoDI GTCTC 5 cut(s) 417, 1029, 1189, 1415, 1580
BcuI ACTAGT 1 cut(s) 1612
BfaI CTAG 2 cut(s) 674, 1613
BfmI CTRYAG 4 cut(s) 609, 894, 1290, 1398
BfoI RGCGCY 1 cut(s) 772
BfuAI ACCTGC 1 cut(s) 1202
BfuI GTATCC 1 cut(s) 1269
BisI GCNGC 4 cut(s) 60, 199, 894, 1052
BlpI GCTNAGC 1 cut(s) 1047
BlsI GCNGC 4 cut(s) 61, 200, 895, 1053
Bme1390I CCNGG 3 cut(s) 45, 191, 1174
Bme18I GGWCC 5 cut(s) 116, 218, 341, 518, 1350
BmgBI CACGTC 1 cut(s) 652
BmgT120I GGNCC 6 cut(s) 116, 218, 283, 341, 518, 1350
BmiI GGNNCC 6 cut(s) 97, 118, 343, 519, 770, 1447
BmrFI CCNGG 3 cut(s) 45, 191, 1174
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 5 cut(s) 440, 1016, 1210, 1272, 1567
BmuI ACTGGG 1 cut(s) 835
BpiI GAAGAC 2 cut(s) 489, 1686
BplI GAGNNNNNCTC 2 cut(s) 1577, 1609
BpmI CTGGAG 2 cut(s) 1163, 1257
Bpu10I CCTNAGC 1 cut(s) 1620
Bpu1102I GCTNAGC 1 cut(s) 1047
BpuEI CTTGAG 1 cut(s) 1579
Bsa29I ATCGAT 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 359
BsaHI GRCGYC 2 cut(s) 231, 769
BsaI GGTCTC 2 cut(s) 417, 1029
BsaJI CCNNGG 6 cut(s) 189, 513, 1039, 1173, 1249, 1449
BsaXI ACNNNNNCTCC 2 cut(s) 1318, 1348
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 292
Bse1I ACTGG 5 cut(s) 97, 381, 391, 830, 1118
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 2 cut(s) 1111, 1191
BseBI CCWGG 3 cut(s) 45, 191, 1174
BseCI ATCGAT 1 cut(s) 67
BseDI CCNNGG 6 cut(s) 189, 513, 1039, 1173, 1249, 1449
BseGI GGATG 1 cut(s) 1159
BseLI CCNNNNNNNGG 2 cut(s) 227, 292
BseMI GCAATG 2 cut(s) 1111, 1191
BseMII CTCAG 4 cut(s) 513, 1038, 1296, 1337
BseNI ACTGG 5 cut(s) 97, 381, 391, 830, 1118
BseRI GAGGAG 1 cut(s) 1598
BseSI GKGCMC 1 cut(s) 1672
BseXI GCAGC 3 cut(s) 210, 880, 1038
BsgI GTGCAG 1 cut(s) 1130
Bsh1236I CGCG 2 cut(s) 23, 971
BshFI GGCC 3 cut(s) 59, 207, 285
BshNI GGYRCC 1 cut(s) 768
BshVI ATCGAT 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 1672
BslFI GGGAC 2 cut(s) 327, 1653
BslI CCNNNNNNNGG 2 cut(s) 227, 292
BsmAI GTCTC 5 cut(s) 417, 1029, 1189, 1415, 1580
BsmFI GGGAC 2 cut(s) 327, 1653
BsnI GGCC 3 cut(s) 59, 207, 285
Bso31I GGTCTC 2 cut(s) 417, 1029
Bsp1286I GDGCHC 1 cut(s) 1672
Bsp143I GATC 4 cut(s) 64, 183, 1488, 1624
Bsp1720I GCTNAGC 1 cut(s) 1047
Bsp19I CCATGG 2 cut(s) 1249, 1449
BspACI CCGC 9 cut(s) 21, 60, 114, 380, 971, 1549, 1564, 1580, 1665
BspANI GGCC 3 cut(s) 59, 207, 285
BspCNI CTCAG 4 cut(s) 514, 1039, 1297, 1338
BspDI ATCGAT 1 cut(s) 67
BspFNI CGCG 2 cut(s) 23, 971
BspLI GGNNCC 6 cut(s) 97, 118, 343, 519, 770, 1447
BspMAI CTGCAG 2 cut(s) 613, 898
BspMI ACCTGC 1 cut(s) 1202
BspPI GGATC 2 cut(s) 191, 1632
BspT107I GGYRCC 1 cut(s) 768
BspTNI GGTCTC 2 cut(s) 417, 1029
BsrDI GCAATG 2 cut(s) 1111, 1191
BsrI ACTGG 5 cut(s) 97, 381, 391, 830, 1118
BssECI CCNNGG 6 cut(s) 189, 513, 1039, 1173, 1249, 1449
BssMI GATC 4 cut(s) 64, 183, 1488, 1624
BssNI GRCGYC 2 cut(s) 231, 769
BssSI CACGAG 2 cut(s) 905, 1029
BssT1I CCWWGG 4 cut(s) 513, 1039, 1249, 1449
Bst2BI CACGAG 2 cut(s) 905, 1029
Bst2UI CCWGG 3 cut(s) 45, 191, 1174
Bst4CI ACNGT 7 cut(s) 169, 218, 619, 1291, 1409, 1544, 1644
Bst6I CTCTTC 1 cut(s) 1503
BstACI GRCGYC 2 cut(s) 231, 769
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 287
BstDEI CTNAG 7 cut(s) 54, 321, 522, 1047, 1305, 1346, 1620
BstDSI CCRYGG 2 cut(s) 1249, 1449
BstF5I GGATG 1 cut(s) 1159
BstFNI CGCG 2 cut(s) 23, 971
BstH2I RGCGCY 1 cut(s) 772
BstHHI GCGC 2 cut(s) 771, 971
BstKTI GATC 4 cut(s) 67, 186, 1491, 1627
BstMAI GTCTC 5 cut(s) 417, 1029, 1189, 1415, 1580
BstMBI GATC 4 cut(s) 64, 183, 1488, 1624
BstMWI GCNNNNNNNGC 3 cut(s) 204, 1025, 1208
BstNI CCWGG 3 cut(s) 45, 191, 1174
BstNSI RCATGY 1 cut(s) 1141
BstSCI CCNGG 3 cut(s) 43, 189, 1172
BstSFI CTRYAG 4 cut(s) 609, 894, 1290, 1398
BstSLI GKGCMC 1 cut(s) 1672
BstUI CGCG 2 cut(s) 23, 971
BstV1I GCAGC 3 cut(s) 210, 880, 1038
BstV2I GAAGAC 2 cut(s) 489, 1686
BstX2I RGATCY 1 cut(s) 1624
BstYI RGATCY 1 cut(s) 1624
Bsu15I ATCGAT 1 cut(s) 67
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 1269
BsuRI GGCC 3 cut(s) 59, 207, 285
BsuTUI ATCGAT 1 cut(s) 67
BtgI CCRYGG 2 cut(s) 1249, 1449
BtgZI GCGATG 1 cut(s) 1034
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 1 cut(s) 1159
BtsI GCAGTG 2 cut(s) 606, 903
BtsIMutI CAGTG 4 cut(s) 606, 615, 903, 1125
BveI ACCTGC 1 cut(s) 1202
Cac8I GCNNGC 1 cut(s) 287
CaiI CAGNNNCTG 2 cut(s) 617, 1217
CfoI GCGC 2 cut(s) 771, 971
Cfr13I GGNCC 6 cut(s) 116, 218, 283, 341, 518, 1350
ClaI ATCGAT 1 cut(s) 67
CseI GACGC 1 cut(s) 220
Csp6I GTAC 3 cut(s) 333, 542, 902
CspCI CAANNNNNGTGG 2 cut(s) 705, 740
CviAII CATG 9 cut(s) 209, 419, 428, 947, 1138, 1150, 1228, 1250, 1450
CviQI GTAC 3 cut(s) 333, 542, 902
DdeI CTNAG 7 cut(s) 54, 321, 522, 1047, 1305, 1346, 1620
DinI GGCGCC 1 cut(s) 770
DpnI GATC 4 cut(s) 66, 185, 1490, 1626
DpnII GATC 4 cut(s) 64, 183, 1488, 1624
Eam1104I CTCTTC 1 cut(s) 1503
EarI CTCTTC 1 cut(s) 1503
EciI GGCGGA 2 cut(s) 395, 1538
Eco130I CCWWGG 4 cut(s) 513, 1039, 1249, 1449
Eco31I GGTCTC 2 cut(s) 417, 1029
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 5 cut(s) 116, 218, 341, 518, 1350
Eco57I CTGAAG 1 cut(s) 245
Eco72I CACGTG 1 cut(s) 359
Eco81I CCTNAGG 1 cut(s) 522
EcoO109I RGGNCCY 2 cut(s) 518, 1350
EcoRII CCWGG 3 cut(s) 43, 189, 1172
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 4 cut(s) 513, 1039, 1249, 1449
EcoT22I ATGCAT 2 cut(s) 433, 1287
EgeI GGCGCC 1 cut(s) 770
EheI GGCGCC 1 cut(s) 770
ErhI CCWWGG 4 cut(s) 513, 1039, 1249, 1449
FaeI CATG 9 cut(s) 212, 422, 431, 950, 1141, 1153, 1231, 1253, 1453
FaqI GGGAC 2 cut(s) 327, 1653
FatI CATG 9 cut(s) 208, 418, 427, 946, 1137, 1149, 1227, 1249, 1449
FauI CCCGC 1 cut(s) 28
FauNDI CATATG 1 cut(s) 1365
FblI GTMKAC 1 cut(s) 1315
Fnu4HI GCNGC 4 cut(s) 60, 199, 894, 1052
FokI GGATG 1 cut(s) 1146
Fsp4HI GCNGC 4 cut(s) 60, 199, 894, 1052
FspBI CTAG 2 cut(s) 674, 1613
GlaI GCGC 2 cut(s) 770, 970
GluI GCNGC 4 cut(s) 60, 199, 894, 1052
GsuI CTGGAG 2 cut(s) 1163, 1257
HaeII RGCGCY 1 cut(s) 772
HaeIII GGCC 3 cut(s) 59, 207, 285
HgaI GACGC 1 cut(s) 220
HhaI GCGC 2 cut(s) 771, 971
Hin1I GRCGYC 2 cut(s) 231, 769
Hin1II CATG 9 cut(s) 212, 422, 431, 950, 1141, 1153, 1231, 1253, 1453
Hin6I GCGC 2 cut(s) 769, 969
HinP1I GCGC 2 cut(s) 769, 969
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 7 cut(s) 7, 238, 245, 553, 974, 1307, 1691
HphI GGTGA 3 cut(s) 178, 674, 1691
Hpy166II GTNNAC 6 cut(s) 214, 628, 850, 904, 1316, 1670
Hpy188I TCNGA 5 cut(s) 268, 304, 441, 1012, 1189
Hpy188III TCNNGA 5 cut(s) 235, 242, 521, 1236, 1596
Hpy8I GTNNAC 6 cut(s) 214, 628, 850, 904, 1316, 1670
Hpy99I CGWCG 2 cut(s) 236, 401
HpyAV CCTTC 7 cut(s) 286, 560, 816, 1089, 1261, 1507, 1718
HpyCH4III ACNGT 7 cut(s) 169, 218, 619, 1291, 1409, 1544, 1644
HpyCH4IV ACGT 5 cut(s) 358, 396, 403, 651, 780
HpyF10VI GCNNNNNNNGC 3 cut(s) 204, 1025, 1208
HpyF3I CTNAG 7 cut(s) 54, 321, 522, 1047, 1305, 1346, 1620
HpySE526I ACGT 5 cut(s) 358, 396, 403, 651, 780
Hsp92I GRCGYC 2 cut(s) 231, 769
Hsp92II CATG 9 cut(s) 212, 422, 431, 950, 1141, 1153, 1231, 1253, 1453
HspAI GCGC 2 cut(s) 769, 969
KasI GGCGCC 1 cut(s) 768
Kzo9I GATC 4 cut(s) 64, 183, 1488, 1624
LmnI GCTCC 1 cut(s) 1043
Lsp1109I GCAGC 3 cut(s) 210, 880, 1038
LweI GCATC 5 cut(s) 440, 1016, 1210, 1272, 1567
MaeI CTAG 2 cut(s) 674, 1613
MaeII ACGT 5 cut(s) 358, 396, 403, 651, 780
MaeIII GTNAC 3 cut(s) 414, 662, 1648
MalI GATC 4 cut(s) 66, 185, 1490, 1626
MboI GATC 4 cut(s) 64, 183, 1488, 1624
MboII GAAGA 6 cut(s) 135, 494, 842, 980, 1520, 1686
MfeI CAATTG 3 cut(s) 177, 1056, 1197
MflI RGATCY 1 cut(s) 1624
MhlI GDGCHC 1 cut(s) 1672
Mly113I GGCGCC 1 cut(s) 769
MlyI GAGTC 4 cut(s) 239, 968, 1316, 1700
Mph1103I ATGCAT 2 cut(s) 433, 1287
MslI CAYNNNNRTG 2 cut(s) 1454, 1559
MspA1I CMGCKG 1 cut(s) 62
MspR9I CCNGG 3 cut(s) 45, 191, 1174
MunI CAATTG 3 cut(s) 177, 1056, 1197
MvaI CCWGG 3 cut(s) 45, 191, 1174
MvnI CGCG 2 cut(s) 23, 971
MwoI GCNNNNNNNGC 3 cut(s) 204, 1025, 1208
NarI GGCGCC 1 cut(s) 769
NcoI CCATGG 2 cut(s) 1249, 1449
NdeI CATATG 1 cut(s) 1365
NdeII GATC 4 cut(s) 64, 183, 1488, 1624
NlaIII CATG 9 cut(s) 212, 422, 431, 950, 1141, 1153, 1231, 1253, 1453
NlaIV GGNNCC 6 cut(s) 97, 118, 343, 519, 770, 1447
NmuCI GTSAC 2 cut(s) 414, 662
NsiI ATGCAT 2 cut(s) 433, 1287
NspI RCATGY 1 cut(s) 1141
PaqCI CACCTGC 1 cut(s) 1202
PfeI GAWTC 2 cut(s) 238, 553
PflFI GACNNNGTC 1 cut(s) 1306
PkrI GCNGC 4 cut(s) 61, 200, 895, 1053
PleI GAGTC 4 cut(s) 239, 968, 1315, 1699
PluTI GGCGCC 1 cut(s) 772
PmaCI CACGTG 1 cut(s) 359
PmlI CACGTG 1 cut(s) 359
PpsI GAGTC 4 cut(s) 239, 968, 1315, 1699
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 2 cut(s) 518, 1350
Psp5II RGGWCCY 2 cut(s) 518, 1350
Psp6I CCWGG 3 cut(s) 43, 189, 1172
PspCI CACGTG 1 cut(s) 359
PspGI CCWGG 3 cut(s) 43, 189, 1172
PspN4I GGNNCC 6 cut(s) 97, 118, 343, 519, 770, 1447
PspPI GGNCC 6 cut(s) 116, 218, 283, 341, 518, 1350
PspPPI RGGWCCY 2 cut(s) 518, 1350
PstI CTGCAG 2 cut(s) 613, 898
PstNI CAGNNNCTG 2 cut(s) 617, 1217
PsuI RGATCY 1 cut(s) 1624
PsyI GACNNNGTC 1 cut(s) 1306
RsaI GTAC 3 cut(s) 334, 543, 903
RsaNI GTAC 3 cut(s) 333, 542, 902
RseI CAYNNNNRTG 2 cut(s) 1454, 1559
SatI GCNGC 4 cut(s) 60, 199, 894, 1052
Sau3AI GATC 4 cut(s) 64, 183, 1488, 1624
Sau96I GGNCC 6 cut(s) 116, 218, 283, 341, 518, 1350
SchI GAGTC 4 cut(s) 239, 968, 1316, 1700
ScrFI CCNGG 3 cut(s) 45, 191, 1174
SduI GDGCHC 1 cut(s) 1672
SfaNI GCATC 5 cut(s) 440, 1016, 1210, 1272, 1567
SfcI CTRYAG 4 cut(s) 609, 894, 1290, 1398
SfoI GGCGCC 1 cut(s) 770
SinI GGWCC 5 cut(s) 116, 218, 341, 518, 1350
SmiMI CAYNNNNRTG 2 cut(s) 1454, 1559
SmlI CTYRAG 1 cut(s) 1594
SmoI CTYRAG 1 cut(s) 1594
SpeI ACTAGT 1 cut(s) 1612
SsiI CCGC 9 cut(s) 21, 60, 114, 380, 971, 1549, 1564, 1580, 1665
SspDI GGCGCC 1 cut(s) 768
SspMI CTAG 2 cut(s) 674, 1613
StyD4I CCNGG 3 cut(s) 43, 189, 1172
StyI CCWWGG 4 cut(s) 513, 1039, 1249, 1449
TaaI ACNGT 7 cut(s) 169, 218, 619, 1291, 1409, 1544, 1644
TaiI ACGT 5 cut(s) 361, 399, 406, 654, 783
TaqI TCGA 3 cut(s) 67, 108, 347
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 2 cut(s) 238, 553
TscAI CASTG 4 cut(s) 613, 622, 903, 1125
TseFI GTSAC 2 cut(s) 414, 662
TseI GCWGC 3 cut(s) 198, 893, 1051
Tsp45I GTSAC 2 cut(s) 414, 662
TspDTI ATGAA 3 cut(s) 407, 935, 980
TspGWI ACGGA 2 cut(s) 88, 1251
TspRI CASTG 4 cut(s) 613, 622, 903, 1125
Tth111I GACNNNGTC 1 cut(s) 1306
VneI GTGCAC 1 cut(s) 1668
VpaK11BI GGWCC 5 cut(s) 116, 218, 341, 518, 1350
XceI RCATGY 1 cut(s) 1141
XmiI GTMKAC 1 cut(s) 1315
XspI CTAG 2 cut(s) 674, 1613
Zsp2I ATGCAT 2 cut(s) 433, 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.