FvH4_5g21480

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001739
Physical Location & Seq
Forward (+)
12943719 .. 12951388
7670 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21480.t3

Sequence Viewer

Length: 1734 bp
ATGACGACTCTTAATGTTCCGGCCAGGATGTCCCTGAAGCCTGACCGGACATGGTCCACTGATCGGTTGGCTTCCTCCAAGAGTTTGGGAGAATCTATTGTCTTTGACTACAGACCCAAGAGAGAAAATGACCGGAAGCAAATGTTCAGTGTTAGATCAGTGAGGATCACCCAGCAGAGCAGTGGAGGCAGTCCTGCCTCTCCTAATGGCCCTGTTGGTGGTGTTGGGGTTAACTTGACTTATGAAATTCGGTCTTATGATGATTACCATGTGGGTCAGGGAAGGGCATTTGCCTGTGGCCGAAGAAAAACTAAGATAGTCTGTACCATTGGTCCTTCCACAAGCTCACGTGAAATGATATGGAAACTTGCAGAAACTGGGATGAATGTAGCGCGTTTGAATATGTCTCACGGGGACCATACGTCGCACCAGAAAACCATTGATCTTGTCAAAGAGTACAACTCTCAATTCAAAGACAAGGTTATAGCCATAATGCTGGACACCAAGGGTCCTGAGGTTAGGAGTGGGGATGTGCCTCAACCAATTCTGCTTGCAGAGGGACAAGAGCTCAACTTTACAATTAAAAGAGGAGTTTGCACAAAAGACACTGTTAGTGTAAACTATGATGACTTTGTGAATGATGTGGAGGTTGGAGACATAGTTCTGGTTGATGGTGGGATGATGTCTTTAGCTGTGAAGTCAAAGACAAAAGATGTGGTAAAATGTTTAGTAGTTGATGGAGGAGAACTAAAATCAAGGCGCCATTTAAATGTGCGTGGAAAAAGTGCAACTCTGCCTTCAATAACAGAAAAAGACTGGGAAGATATCAAGTTTGGAGTGGACAATGGAGTTGATTTCTATGCTGTCTCATTCGTAAAAAGTGCTGCGGTGGTTCATGAGTTGAAAGATTATCTCAAAGGTTGTAATGCAGATATTCATGTTATTGTAAAAATTGAAAGTGCAGACTCTATACCAAATCTTCAATCAATAATTTCTGCATCTGATGGGGCTATGATTGCTCGTGGAGACCTTGGAGCTGAGCTTCCAATTGAGGAAGTTCCTTTGTTGCAGGAAGACATCATTAGAAGGTGTCACACTATGCAGAAACCAGTTATTGTAGCAACTAATATGTTGGAGAGCATGATCGATCATCCTACACCAACCAGGGCAGAAGTTTCTGACATTGCAATTGCAGTGAGAGAAGGTGCCGATGCCATCATGCTTTCTGGAGAAACTGCCCATGGAAAATATCCACTCAAAGCTGTTAAAGTAATGCATACTGTGGCATTGAGGACTGAGTCAAGTCTACCAATTAGCACTATTCCTCCGATCCAATTAAGTGCCTACAAGAGACATATGGGGGAAATGTTTGCTTTCCACTCTACAATTATGGCCAACAGTCTCAATACACCCATCATTGTTTTTACAAGGACAGGATCCATGGCTGTACTCTTAAGCCATTATAGGCCATGCTCCACTATCTTTGCCTTCACAAATGAAGAAAGGATTAAGCAGAGATTGGTGCTTTATCAAGGTGTCCTCCCAATATATATGCAATTCTCAAATGATTCAGAGGAGACTTACTCAAGAGCAATACAGCTATTAGTGGCTAAGGGACATTTGAAAGAGGGAGAGCATGTTACTCTTGTCCAAAGTGGCGCACAACCAATCTGGCGTAAAGAATCTTCTCATCATATCCAAGTTCGTAAGGTGGAAAGTATTTCGAACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

578

Amino Acids

63.77

Weight (kDa)

8.3

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 102 - 422 9e-112 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 759, 1205
AccI GTMKAC 1 cut(s) 1306
AccII CGCG 1 cut(s) 394
AciI CCGC 1 cut(s) 887
AclWI GGATC 4 cut(s) 173, 1324, 1431, 1444
AcoI YGGCCR 3 cut(s) 21, 298, 1392
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 56
AcvI CACGTG 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 760
AfaI GTAC 3 cut(s) 325, 458, 1449
AfiI CCNNNNNNNGG 2 cut(s) 63, 218
AflII CTTAAG 1 cut(s) 1453
AgsI TTSAA 7 cut(s) 400, 472, 801, 904, 956, 983, 1624
AhdI GACNNNNNGTC 1 cut(s) 421
AjnI CCWGG 2 cut(s) 23, 1163
AloI GAACNNNNNNTCC 2 cut(s) 645, 677
AluBI AGCT 7 cut(s) 345, 568, 692, 1037, 1042, 1262, 1600
AluI AGCT 7 cut(s) 345, 568, 692, 1037, 1042, 1262, 1600
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 7 cut(s) 411, 648, 871, 1020, 1345, 1406, 1571
AlwI GGATC 4 cut(s) 173, 1324, 1431, 1444
AlwNI CAGNNNCTG 1 cut(s) 377
AoxI GGCC 5 cut(s) 21, 208, 298, 1392, 1466
ApeKI GCWGC 1 cut(s) 884
ApoI RAATTY 1 cut(s) 246
AspLEI GCGC 3 cut(s) 394, 762, 1661
AspS9I GGNCC 5 cut(s) 54, 209, 332, 415, 509
AsuHPI GGTGA 1 cut(s) 160
AsuII TTCGAA 1 cut(s) 1724
AvaII GGWCC 4 cut(s) 54, 332, 415, 509
AxyI CCTNAGG 1 cut(s) 513
BalI TGGCCA 1 cut(s) 1394
BamHI GGATCC 1 cut(s) 1436
BanI GGYRCC 2 cut(s) 759, 1205
BanII GRGCYC 1 cut(s) 570
BarI GAAGNNNNNNTAC 2 cut(s) 1669, 1701
BauI CACGAG 1 cut(s) 1020
BbrPI CACGTG 1 cut(s) 350
BbsI GAAGAC 1 cut(s) 1080
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 1 cut(s) 871
BccI CCATC 5 cut(s) 665, 731, 998, 1223, 1421
BciT130I CCWGG 2 cut(s) 25, 1165
BcoDI GTCTC 7 cut(s) 411, 648, 871, 1020, 1345, 1406, 1571
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 1 cut(s) 763
BfrI CTTAAG 1 cut(s) 1453
BisI GCNGC 1 cut(s) 885
BlpI GCTNAGC 1 cut(s) 1038
BlsI GCNGC 1 cut(s) 886
Bme1390I CCNGG 2 cut(s) 25, 1165
Bme18I GGWCC 4 cut(s) 54, 332, 415, 509
BmeRI GACNNNNNGTC 1 cut(s) 421
BmgT120I GGNCC 5 cut(s) 54, 209, 332, 415, 509
BmiI GGNNCC 5 cut(s) 416, 510, 761, 1207, 1438
BmrFI CCNGG 2 cut(s) 25, 1165
BmrI ACTGGG 2 cut(s) 387, 826
BmsI GCATC 2 cut(s) 1007, 1201
BmuI ACTGGG 2 cut(s) 387, 826
BpiI GAAGAC 1 cut(s) 1080
BplI GAGNNNNNCTC 4 cut(s) 446, 478, 1568, 1600
BpmI CTGGAG 1 cut(s) 1248
Bpu10I CCTNAGC 1 cut(s) 1611
Bpu1102I GCTNAGC 1 cut(s) 1038
Bpu14I TTCGAA 1 cut(s) 1724
BpuEI CTTGAG 1 cut(s) 1570
Bsa29I ATCGAT 1 cut(s) 1146
BsaAI YACGTR 1 cut(s) 350
BsaHI GRCGYC 1 cut(s) 760
BsaI GGTCTC 1 cut(s) 1020
BsaJI CCNNGG 5 cut(s) 504, 1030, 1164, 1240, 1440
BsaWI WCCGGW 2 cut(s) 45, 132
BsaXI ACNNNNNCTCC 6 cut(s) 645, 675, 1309, 1339, 1623, 1653
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 218
Bse1I ACTGG 3 cut(s) 382, 821, 1109
Bse21I CCTNAGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 1182
BseBI CCWGG 2 cut(s) 25, 1165
BseCI ATCGAT 1 cut(s) 1146
BseDI CCNNGG 5 cut(s) 504, 1030, 1164, 1240, 1440
BseGI GGATG 5 cut(s) 33, 387, 535, 684, 1150
BseLI CCNNNNNNNGG 2 cut(s) 63, 218
BseMI GCAATG 1 cut(s) 1182
BseMII CTCAG 3 cut(s) 504, 1029, 1287
BseNI ACTGG 3 cut(s) 382, 821, 1109
BseRI GAGGAG 3 cut(s) 603, 756, 1589
BseXI GCAGC 1 cut(s) 871
BseYI CCCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 981
Bsh1236I CGCG 1 cut(s) 394
BshFI GGCC 5 cut(s) 23, 210, 300, 1394, 1468
BshNI GGYRCC 2 cut(s) 759, 1205
BshVI ATCGAT 1 cut(s) 1146
BsiHKAI GWGCWC 1 cut(s) 570
BsiSI CCGG 3 cut(s) 20, 46, 133
BslFI GGGAC 4 cut(s) 16, 428, 573, 1629
BslI CCNNNNNNNGG 2 cut(s) 63, 218
BsmAI GTCTC 7 cut(s) 411, 648, 871, 1020, 1345, 1406, 1571
BsmFI GGGAC 4 cut(s) 16, 428, 573, 1629
BsnI GGCC 5 cut(s) 23, 210, 300, 1394, 1468
Bso31I GGTCTC 1 cut(s) 1020
Bsp119I TTCGAA 1 cut(s) 1724
Bsp1286I GDGCHC 1 cut(s) 570
Bsp143I GATC 8 cut(s) 61, 155, 165, 442, 1143, 1147, 1329, 1436
Bsp1720I GCTNAGC 1 cut(s) 1038
Bsp19I CCATGG 2 cut(s) 1240, 1440
BspACI CCGC 1 cut(s) 887
BspANI GGCC 5 cut(s) 23, 210, 300, 1394, 1468
BspCNI CTCAG 3 cut(s) 505, 1030, 1288
BspDI ATCGAT 1 cut(s) 1146
BspFNI CGCG 1 cut(s) 394
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 5 cut(s) 416, 510, 761, 1207, 1438
BspPI GGATC 4 cut(s) 173, 1324, 1431, 1444
BspT104I TTCGAA 1 cut(s) 1724
BspT107I GGYRCC 2 cut(s) 759, 1205
BspTI CTTAAG 1 cut(s) 1453
BspTNI GGTCTC 1 cut(s) 1020
BsrDI GCAATG 1 cut(s) 1182
BsrI ACTGG 3 cut(s) 382, 821, 1109
BssECI CCNNGG 5 cut(s) 504, 1030, 1164, 1240, 1440
BssMI GATC 8 cut(s) 61, 155, 165, 442, 1143, 1147, 1329, 1436
BssNI GRCGYC 1 cut(s) 760
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 4 cut(s) 504, 1030, 1240, 1440
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 2 cut(s) 25, 1165
Bst4CI ACNGT 3 cut(s) 610, 1282, 1400
BstACI GRCGYC 1 cut(s) 760
BstAFI CTTAAG 1 cut(s) 1453
BstBAI YACGTR 1 cut(s) 350
BstBI TTCGAA 1 cut(s) 1724
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 5 cut(s) 312, 513, 1038, 1296, 1611
BstDSI CCRYGG 2 cut(s) 1240, 1440
BstF5I GGATG 5 cut(s) 33, 387, 535, 684, 1150
BstFNI CGCG 1 cut(s) 394
BstH2I RGCGCY 1 cut(s) 763
BstHHI GCGC 3 cut(s) 394, 762, 1661
BstKTI GATC 8 cut(s) 64, 158, 168, 445, 1146, 1150, 1332, 1439
BstMAI GTCTC 7 cut(s) 411, 648, 871, 1020, 1345, 1406, 1571
BstMBI GATC 8 cut(s) 61, 155, 165, 442, 1143, 1147, 1329, 1436
BstMWI GCNNNNNNNGC 2 cut(s) 186, 1016
BstNI CCWGG 2 cut(s) 25, 1165
BstNSI RCATGY 1 cut(s) 1640
BstSCI CCNGG 2 cut(s) 23, 1163
BstSFI CTRYAG 1 cut(s) 109
BstUI CGCG 1 cut(s) 394
BstV1I GCAGC 1 cut(s) 871
BstV2I GAAGAC 1 cut(s) 1080
BstX2I RGATCY 1 cut(s) 1436
BstXI CCANNNNNNTGG 2 cut(s) 85, 496
BstYI RGATCY 1 cut(s) 1436
Bsu15I ATCGAT 1 cut(s) 1146
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 5 cut(s) 23, 210, 300, 1394, 1468
BsuTUI ATCGAT 1 cut(s) 1146
BtgI CCRYGG 2 cut(s) 1240, 1440
BtsCI GGATG 5 cut(s) 33, 387, 535, 684, 1150
BtsI GCAGTG 2 cut(s) 187, 1200
BtsIMutI CAGTG 6 cut(s) 57, 154, 165, 187, 606, 1200
Cac8I GCNNGC 1 cut(s) 552
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 1 cut(s) 895
CfoI GCGC 3 cut(s) 394, 762, 1661
Cfr13I GGNCC 5 cut(s) 54, 209, 332, 415, 509
ClaI ATCGAT 1 cut(s) 1146
Csp6I GTAC 3 cut(s) 324, 457, 1448
CspCI CAANNNNNGTGG 4 cut(s) 696, 731, 1465, 1500
CviQI GTAC 3 cut(s) 324, 457, 1448
DdeI CTNAG 5 cut(s) 312, 513, 1038, 1296, 1611
DinI GGCGCC 1 cut(s) 761
DpnI GATC 8 cut(s) 63, 157, 167, 444, 1145, 1149, 1331, 1438
DpnII GATC 8 cut(s) 61, 155, 165, 442, 1143, 1147, 1329, 1436
DraI TTTAAA 1 cut(s) 768
DriI GACNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 3 cut(s) 21, 298, 1392
Eam1105I GACNNNNNGTC 1 cut(s) 421
Ecl136II GAGCTC 1 cut(s) 568
Eco130I CCWWGG 4 cut(s) 504, 1030, 1240, 1440
Eco24I GRGCYC 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 1020
Eco32I GATATC 1 cut(s) 826
Eco47I GGWCC 4 cut(s) 54, 332, 415, 509
Eco53kI GAGCTC 1 cut(s) 568
Eco57I CTGAAG 1 cut(s) 56
Eco72I CACGTG 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 568
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 2 cut(s) 23, 1163
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 4 cut(s) 504, 1030, 1240, 1440
EcoT22I ATGCAT 1 cut(s) 1278
EcoT38I GRGCYC 1 cut(s) 570
EgeI GGCGCC 1 cut(s) 761
EheI GGCGCC 1 cut(s) 761
ErhI CCWWGG 4 cut(s) 504, 1030, 1240, 1440
FaqI GGGAC 4 cut(s) 16, 428, 573, 1629
FauNDI CATATG 1 cut(s) 1356
FblI GTMKAC 1 cut(s) 1306
Fnu4HI GCNGC 1 cut(s) 885
FokI GGATG 5 cut(s) 40, 394, 542, 691, 1137
FriOI GRGCYC 1 cut(s) 570
Fsp4HI GCNGC 1 cut(s) 885
GlaI GCGC 3 cut(s) 393, 761, 1660
GluI GCNGC 1 cut(s) 885
GsaI CCCAGC 1 cut(s) 175
GsuI CTGGAG 1 cut(s) 1248
HaeII RGCGCY 1 cut(s) 763
HaeIII GGCC 5 cut(s) 23, 210, 300, 1394, 1468
HapII CCGG 3 cut(s) 20, 46, 133
HhaI GCGC 3 cut(s) 394, 762, 1661
Hin1I GRCGYC 1 cut(s) 760
Hin6I GCGC 3 cut(s) 392, 760, 1659
HinP1I GCGC 3 cut(s) 392, 760, 1659
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HinfI GANTC 6 cut(s) 7, 92, 965, 1298, 1568, 1682
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 3 cut(s) 20, 46, 133
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 5 cut(s) 57, 232, 619, 841, 1307
Hpy188I TCNGA 4 cut(s) 1003, 1180, 1329, 1573
Hpy188III TCNNGA 4 cut(s) 512, 896, 1227, 1587
Hpy8I GTNNAC 5 cut(s) 57, 232, 619, 841, 1307
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 6 cut(s) 276, 345, 807, 1080, 1196, 1498
HpyCH4III ACNGT 3 cut(s) 610, 1282, 1400
HpyCH4IV ACGT 2 cut(s) 349, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 1016
HpyF3I CTNAG 5 cut(s) 312, 513, 1038, 1296, 1611
HpySE526I ACGT 2 cut(s) 349, 422
Hsp92I GRCGYC 1 cut(s) 760
HspAI GCGC 3 cut(s) 392, 760, 1659
KasI GGCGCC 1 cut(s) 759
KspAI GTTAAC 1 cut(s) 232
Kzo9I GATC 8 cut(s) 61, 155, 165, 442, 1143, 1147, 1329, 1436
LmnI GCTCC 2 cut(s) 1034, 1478
Lsp1109I GCAGC 1 cut(s) 871
LweI GCATC 2 cut(s) 1007, 1201
MaeII ACGT 2 cut(s) 349, 422
MaeIII GTNAC 2 cut(s) 1091, 1639
MalI GATC 8 cut(s) 63, 157, 167, 444, 1145, 1149, 1331, 1438
MboI GATC 8 cut(s) 61, 155, 165, 442, 1143, 1147, 1329, 1436
MboII GAAGA 6 cut(s) 315, 833, 971, 1085, 1511, 1677
MfeI CAATTG 2 cut(s) 1047, 1188
MflI RGATCY 1 cut(s) 1436
MhlI GDGCHC 1 cut(s) 570
MlsI TGGCCA 1 cut(s) 1394
MluNI TGGCCA 1 cut(s) 1394
Mly113I GGCGCC 1 cut(s) 760
MlyI GAGTC 2 cut(s) 959, 1307
MmeI TCCRAC 2 cut(s) 631, 1113
Mox20I TGGCCA 1 cut(s) 1394
Mph1103I ATGCAT 1 cut(s) 1278
MscI TGGCCA 1 cut(s) 1394
MseI TTAA 8 cut(s) 12, 231, 582, 767, 1266, 1337, 1454, 1509
MslI CAYNNNNRTG 1 cut(s) 768
Msp20I TGGCCA 1 cut(s) 1394
MspCI CTTAAG 1 cut(s) 1453
MspI CCGG 3 cut(s) 20, 46, 133
MspR9I CCNGG 2 cut(s) 25, 1165
MunI CAATTG 2 cut(s) 1047, 1188
MvaI CCWGG 2 cut(s) 25, 1165
MvnI CGCG 1 cut(s) 394
MwoI GCNNNNNNNGC 2 cut(s) 186, 1016
NarI GGCGCC 1 cut(s) 760
NcoI CCATGG 2 cut(s) 1240, 1440
NdeI CATATG 1 cut(s) 1356
NdeII GATC 8 cut(s) 61, 155, 165, 442, 1143, 1147, 1329, 1436
NlaIV GGNNCC 5 cut(s) 416, 510, 761, 1207, 1438
NmuCI GTSAC 1 cut(s) 1091
NsiI ATGCAT 1 cut(s) 1278
NspI RCATGY 1 cut(s) 1640
NspV TTCGAA 1 cut(s) 1724
PagI TCATGA 1 cut(s) 895
PfeI GAWTC 3 cut(s) 92, 1568, 1682
PflFI GACNNNGTC 2 cut(s) 52, 1297
PkrI GCNGC 1 cut(s) 886
PleI GAGTC 2 cut(s) 959, 1306
PluTI GGCGCC 1 cut(s) 763
PmaCI CACGTG 1 cut(s) 350
PmlI CACGTG 1 cut(s) 350
PpsI GAGTC 2 cut(s) 959, 1306
Ppu21I YACGTR 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 509
Psp124BI GAGCTC 1 cut(s) 570
Psp5II RGGWCCY 1 cut(s) 509
Psp6I CCWGG 2 cut(s) 23, 1163
PspCI CACGTG 1 cut(s) 350
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 2 cut(s) 23, 1163
PspN4I GGNNCC 5 cut(s) 416, 510, 761, 1207, 1438
PspPI GGNCC 5 cut(s) 54, 209, 332, 415, 509
PspPPI RGGWCCY 1 cut(s) 509
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 1 cut(s) 1436
PsyI GACNNNGTC 2 cut(s) 52, 1297
RsaI GTAC 3 cut(s) 325, 458, 1449
RsaNI GTAC 3 cut(s) 324, 457, 1448
RseI CAYNNNNRTG 1 cut(s) 768
SacI GAGCTC 1 cut(s) 570
SaqAI TTAA 8 cut(s) 12, 231, 582, 767, 1266, 1337, 1454, 1509
SatI GCNGC 1 cut(s) 885
Sau3AI GATC 8 cut(s) 61, 155, 165, 442, 1143, 1147, 1329, 1436
Sau96I GGNCC 5 cut(s) 54, 209, 332, 415, 509
SchI GAGTC 2 cut(s) 959, 1307
ScrFI CCNGG 2 cut(s) 25, 1165
SduI GDGCHC 1 cut(s) 570
SfaNI GCATC 2 cut(s) 1007, 1201
SfcI CTRYAG 1 cut(s) 109
SfoI GGCGCC 1 cut(s) 761
SfuI TTCGAA 1 cut(s) 1724
SinI GGWCC 4 cut(s) 54, 332, 415, 509
SmiI ATTTAAAT 1 cut(s) 768
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 2 cut(s) 1453, 1585
SmoI CTYRAG 2 cut(s) 1453, 1585
SsiI CCGC 1 cut(s) 887
SspDI GGCGCC 1 cut(s) 759
SstI GAGCTC 1 cut(s) 570
StyD4I CCNGG 2 cut(s) 23, 1163
StyI CCWWGG 4 cut(s) 504, 1030, 1240, 1440
SwaI ATTTAAAT 1 cut(s) 768
TaaI ACNGT 3 cut(s) 610, 1282, 1400
TaiI ACGT 2 cut(s) 352, 425
TaqI TCGA 2 cut(s) 1146, 1724
TaqII GACCGA 1 cut(s) 240
TatI WGTACW 2 cut(s) 456, 1447
TfiI GAWTC 3 cut(s) 92, 1568, 1682
Tru1I TTAA 8 cut(s) 12, 231, 582, 767, 1266, 1337, 1454, 1509
Tru9I TTAA 8 cut(s) 12, 231, 582, 767, 1266, 1337, 1454, 1509
TscAI CASTG 6 cut(s) 64, 154, 165, 187, 613, 1200
TseFI GTSAC 1 cut(s) 1091
TseI GCWGC 1 cut(s) 884
Tsp45I GTSAC 1 cut(s) 1091
TspDTI ATGAA 5 cut(s) 258, 398, 884, 926, 1512
TspRI CASTG 6 cut(s) 64, 154, 165, 187, 613, 1200
Tth111I GACNNNGTC 2 cut(s) 52, 1297
Vha464I CTTAAG 1 cut(s) 1453
VpaK11BI GGWCC 4 cut(s) 54, 332, 415, 509
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 1640
XcmI CCANNNNNNNNNTGG 2 cut(s) 64, 179
XmiI GTMKAC 1 cut(s) 1306
Zsp2I ATGCAT 1 cut(s) 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.