Rh3BG280200

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001739
Physical Location & Seq
Forward (+)
27655226 .. 27662875
7650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG280200.1

Sequence Viewer

Length: 831 bp
ATGGTCCTGTGGGTGGTGTTGGGGGTGAACTCGACTTATGAAATTCAGTCTGATGATGATTACAAAGTGGGTCAGCGAAGGGCATTTGCAAGTGGTCGAAGAAAAACTAAGATTGTCTGTACAATTGGTCCTTACACAAGCTCACGTGAAATGATATGGAAACTTGCAGAAACTGGGATGAATGTAGTGCGTTTGAAAATGTCTCATGGGGACCATACGTCGCACCAGAAAACTATTGATCTTGTCAAAGAATACAACTCTCAATTCAAAGACAAGGTTATAGCCATAATACTGGACACCAAGGGTCCTGAGGTTAGGAGTGGAGATGTACCACAACCAATTCTGCTTACAGAGGGACAAGAGCTTAACTTTACAATTAGAAGAGGAGTTAGCACCAAAGATACCTTCTGGTTGATGGGAAAGCTGAAGGTGTTATATATGAAGATGATGGATCTTGGAAGAGGCCAAACTGTCGCTTGCATGTGCAATTATTGCTTGGTGGAGGTGCCATGGTTGATATATGGGGTGAGGATGGAGAGATTCTTCAACTTTCAATGCCTTCAGAACAGGAGGTCTCACAGCTGGTTTCTACCAGTGAGAAACAGTACAGAACTCGTTTGGATTTTGCTTTCAGCTTTCGAGTGTGCGCAGTCGCAGTGCAATGAGAGGAGGATTCAGCAGAGGAACGCTTACCCGCCCGAAGCGTTGGAACTTGACAACATCTCAAATCAGATTCCAAAGGAGCTGGTAACTACTCCAACTGATGAAACTGACATGATACGCAATCAACCGGGGGGGGGGGGTTTCACACCGAAGGAGCTGGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

276

Amino Acids

31.74

Weight (kDa)

8.78

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 34 - 133 3.7e-27 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 505
AciI CCGC 1 cut(s) 695
AclWI GGATC 1 cut(s) 459
AcsI RAATTY 1 cut(s) 42
AcuI CTGAAG 2 cut(s) 446, 545
AcvI CACGTG 1 cut(s) 146
AfaI GTAC 3 cut(s) 121, 330, 607
AfiI CCNNNNNNNGG 2 cut(s) 13, 797
AgsI TTSAA 4 cut(s) 196, 268, 547, 554
AhdI GACNNNNNGTC 1 cut(s) 217
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AloI GAACNNNNNNTCC 1 cut(s) 809
AluBI AGCT 7 cut(s) 141, 364, 424, 582, 635, 745, 820
AluI AGCT 7 cut(s) 141, 364, 424, 582, 635, 745, 820
Alw26I GTCTC 2 cut(s) 207, 579
AlwI GGATC 1 cut(s) 459
AlwNI CAGNNNCTG 1 cut(s) 173
AoxI GGCC 1 cut(s) 463
ApoI RAATTY 1 cut(s) 42
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 4 cut(s) 4, 128, 211, 305
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 2 cut(s) 37, 538
AvaII GGWCC 4 cut(s) 4, 128, 211, 305
AxyI CCTNAGG 1 cut(s) 309
BanI GGYRCC 1 cut(s) 505
BbrPI CACGTG 1 cut(s) 146
BccI CCATC 3 cut(s) 409, 442, 526
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 2 cut(s) 207, 579
Bme1390I CCNGG 1 cut(s) 792
Bme18I GGWCC 4 cut(s) 4, 128, 211, 305
BmeRI GACNNNNNGTC 1 cut(s) 217
BmgT120I GGNCC 4 cut(s) 4, 128, 211, 305
BmiI GGNNCC 3 cut(s) 212, 306, 507
BmrFI CCNGG 1 cut(s) 792
BmrI ACTGGG 1 cut(s) 183
BmuI ACTGGG 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 792
BsaAI YACGTR 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 579
BsaJI CCNNGG 3 cut(s) 300, 509, 791
BsaXI ACNNNNNCTCC 1 cut(s) 809
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 797
Bse1I ACTGG 3 cut(s) 178, 297, 593
Bse21I CCTNAGG 1 cut(s) 309
Bse3DI GCAATG 1 cut(s) 667
BseDI CCNNGG 3 cut(s) 300, 509, 791
BseGI GGATG 2 cut(s) 183, 537
BseLI CCNNNNNNNGG 2 cut(s) 13, 797
BseMI GCAATG 1 cut(s) 667
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 3 cut(s) 178, 297, 593
BseRI GAGGAG 2 cut(s) 399, 682
BshFI GGCC 1 cut(s) 465
BshNI GGYRCC 1 cut(s) 505
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 2 cut(s) 224, 369
BslI CCNNNNNNNGG 2 cut(s) 13, 797
BsmAI GTCTC 2 cut(s) 207, 579
BsmFI GGGAC 2 cut(s) 224, 369
BsnI GGCC 1 cut(s) 465
Bso31I GGTCTC 1 cut(s) 579
Bsp1407I TGTACA 1 cut(s) 119
Bsp143I GATC 2 cut(s) 238, 451
Bsp19I CCATGG 1 cut(s) 509
BspACI CCGC 1 cut(s) 695
BspANI GGCC 1 cut(s) 465
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 3 cut(s) 212, 306, 507
BspPI GGATC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 505
BspTNI GGTCTC 1 cut(s) 579
BsrDI GCAATG 1 cut(s) 667
BsrGI TGTACA 1 cut(s) 119
BsrI ACTGG 3 cut(s) 178, 297, 593
BssECI CCNNGG 3 cut(s) 300, 509, 791
BssMI GATC 2 cut(s) 238, 451
BssT1I CCWWGG 2 cut(s) 300, 509
Bst4CI ACNGT 2 cut(s) 472, 605
Bst6I CTCTTC 2 cut(s) 376, 454
BstAPI GCANNNNNTGC 1 cut(s) 492
BstAUI TGTACA 1 cut(s) 119
BstBAI YACGTR 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 2 cut(s) 108, 309
BstDSI CCRYGG 1 cut(s) 509
BstF5I GGATG 2 cut(s) 183, 537
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 2 cut(s) 241, 454
BstMAI GTCTC 2 cut(s) 207, 579
BstMBI GATC 2 cut(s) 238, 451
BstMWI GCNNNNNNNGC 1 cut(s) 492
BstNSI RCATGY 1 cut(s) 484
BstSCI CCNGG 1 cut(s) 790
BstX2I RGATCY 1 cut(s) 451
BstXI CCANNNNNNTGG 1 cut(s) 292
BstYI RGATCY 1 cut(s) 451
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 1 cut(s) 465
BtgI CCRYGG 1 cut(s) 509
BtsCI GGATG 2 cut(s) 183, 537
BtsI GCAGTG 1 cut(s) 662
BtsIMutI CAGTG 2 cut(s) 600, 662
Cac8I GCNNGC 1 cut(s) 478
CaiI CAGNNNCTG 1 cut(s) 173
CfoI GCGC 1 cut(s) 649
Cfr13I GGNCC 4 cut(s) 4, 128, 211, 305
Csp6I GTAC 3 cut(s) 120, 329, 606
CviAII CATG 4 cut(s) 206, 481, 510, 775
CviJI RGCY 9 cut(s) 141, 284, 364, 424, 465, 582, 635, 745, 820
CviKI_1 RGCY 9 cut(s) 141, 284, 364, 424, 465, 582, 635, 745, 820
CviQI GTAC 3 cut(s) 120, 329, 606
DdeI CTNAG 2 cut(s) 108, 309
DpnI GATC 2 cut(s) 240, 453
DpnII GATC 2 cut(s) 238, 451
DriI GACNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 376, 454
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 2 cut(s) 376, 454
Eco130I CCWWGG 2 cut(s) 300, 509
Eco31I GGTCTC 1 cut(s) 579
Eco47I GGWCC 4 cut(s) 4, 128, 211, 305
Eco57I CTGAAG 2 cut(s) 446, 545
Eco72I CACGTG 1 cut(s) 146
Eco81I CCTNAGG 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 305
EcoT14I CCWWGG 2 cut(s) 300, 509
ErhI CCWWGG 2 cut(s) 300, 509
FaeI CATG 4 cut(s) 209, 484, 513, 778
FaqI GGGAC 2 cut(s) 224, 369
FatI CATG 4 cut(s) 205, 480, 509, 774
FauI CCCGC 1 cut(s) 702
FokI GGATG 2 cut(s) 190, 544
FspI TGCGCA 1 cut(s) 648
GlaI GCGC 1 cut(s) 648
HaeIII GGCC 1 cut(s) 465
HapII CCGG 1 cut(s) 791
HhaI GCGC 1 cut(s) 649
Hin1II CATG 4 cut(s) 209, 484, 513, 778
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HinfI GANTC 3 cut(s) 540, 673, 733
HpaII CCGG 1 cut(s) 791
HphI GGTGA 2 cut(s) 37, 538
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 3 cut(s) 52, 564, 732
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 5 cut(s) 72, 415, 421, 569, 808
HpyCH4III ACNGT 2 cut(s) 472, 605
HpyCH4IV ACGT 2 cut(s) 145, 218
HpyCH4V TGCA 5 cut(s) 89, 167, 480, 486, 660
HpyF10VI GCNNNNNNNGC 1 cut(s) 492
HpyF3I CTNAG 2 cut(s) 108, 309
HpySE526I ACGT 2 cut(s) 145, 218
Hsp92II CATG 4 cut(s) 209, 484, 513, 778
HspAI GCGC 1 cut(s) 647
Kzo9I GATC 2 cut(s) 238, 451
LmnI GCTCC 2 cut(s) 742, 817
MaeII ACGT 2 cut(s) 145, 218
MaeIII GTNAC 1 cut(s) 748
MalI GATC 2 cut(s) 240, 453
MboI GATC 2 cut(s) 238, 451
MboII GAAGA 5 cut(s) 111, 393, 454, 471, 535
MfeI CAATTG 1 cut(s) 123
MflI RGATCY 1 cut(s) 451
MluCI AATT 6 cut(s) 42, 123, 263, 339, 375, 487
MmeI TCCRAC 2 cut(s) 687, 782
MseI TTAA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 582
MspI CCGG 1 cut(s) 791
MspR9I CCNGG 1 cut(s) 792
MunI CAATTG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 492
NciI CCSGG 1 cut(s) 792
NcoI CCATGG 1 cut(s) 509
NdeII GATC 2 cut(s) 238, 451
NlaIII CATG 4 cut(s) 209, 484, 513, 778
NlaIV GGNNCC 3 cut(s) 212, 306, 507
NsbI TGCGCA 1 cut(s) 648
NspI RCATGY 1 cut(s) 484
PfeI GAWTC 3 cut(s) 540, 673, 733
PmaCI CACGTG 1 cut(s) 146
PmlI CACGTG 1 cut(s) 146
Ppu21I YACGTR 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 305
PspCI CACGTG 1 cut(s) 146
PspN4I GGNNCC 3 cut(s) 212, 306, 507
PspPI GGNCC 4 cut(s) 4, 128, 211, 305
PspPPI RGGWCCY 1 cut(s) 305
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 451
PvuII CAGCTG 1 cut(s) 582
RsaI GTAC 3 cut(s) 121, 330, 607
RsaNI GTAC 3 cut(s) 120, 329, 606
SaqAI TTAA 1 cut(s) 366
Sau3AI GATC 2 cut(s) 238, 451
Sau96I GGNCC 4 cut(s) 4, 128, 211, 305
ScrFI CCNGG 1 cut(s) 792
SinI GGWCC 4 cut(s) 4, 128, 211, 305
Sse9I AATT 6 cut(s) 42, 123, 263, 339, 375, 487
SsiI CCGC 1 cut(s) 695
StyD4I CCNGG 1 cut(s) 790
StyI CCWWGG 2 cut(s) 300, 509
TaaI ACNGT 2 cut(s) 472, 605
TaiI ACGT 2 cut(s) 148, 221
TaqI TCGA 3 cut(s) 32, 97, 639
TasI AATT 6 cut(s) 42, 123, 263, 339, 375, 487
TatI WGTACW 2 cut(s) 119, 605
TfiI GAWTC 3 cut(s) 540, 673, 733
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 2 cut(s) 600, 662
TspDTI ATGAA 4 cut(s) 54, 194, 455, 780
TspRI CASTG 2 cut(s) 600, 662
VpaK11BI GGWCC 4 cut(s) 4, 128, 211, 305
XapI RAATTY 1 cut(s) 42
XceI RCATGY 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.