Rroxscaffold_2G00090500

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001739
Physical Location & Seq
Reverse (-)
12244377 .. 12247226
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090500.1

Sequence Viewer

Length: 684 bp
ATGTTCACTGTTAGATCAGTGAGGATCACCCAGCAGAGCAGTGAAGGCAATCCTAGCTCACCCAATGGTCCTATGGGTGCTGTTGAAGTTGGTCAGGGAAGGGCATTTGCAAGTGGTCGAAGAAAAACTAAGATTGTCTGTACAATTGGTCCTTCCACAAGCTCACGTGAAATGATATGGAAACTTGCAGAAACTGGGATGAATGTAGTGCGTTTGAATATGTCTCATGGGGACCATACGTCGCACCAGAAAACTATTGATCTTGTCAAAGAATACAACTCTCAATTCAAAGACAAGGTTATAGCCATAATGCTGGACACCAAGGGTCCTGAGGTTAGGAGTGGAGATGTACCACAACCAATTCTGCTTACAGAGGGACAAGAGTTTAACTTTACAATTAGAAGAGGAGTTAGCACAAAAGATACCGTTAGTGTAAACTATGATGACTTTGTGAATGATGTGGCGGTTGGAGACATAGTTCTGGTTGATGAAAAAGACTGGGAAGATATCAAGTTTGGAGTGGACAACGAAGTTGATTTCTATGCCATCTCATTTGTAAAAAGTGCTGCGGTGAAAGATCATCTCAAAGGTTGTAATGCAGATATTCATGCTATCGTAAAAATTGAAAGTGCAGACTCTATACCAAATCTTGATTTCCTTTTTTGTTCAAAACAAGCACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

227

Amino Acids

25.01

Weight (kDa)

5.57

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 41 - 164 4.2e-35 Pyruvate kinase, barrel domain
PK PF00224 163 - 218 9.6e-08 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 464, 569
AclWI GGATC 1 cut(s) 32
AcvI CACGTG 1 cut(s) 167
AfaI GTAC 2 cut(s) 142, 351
AgsI TTSAA 5 cut(s) 86, 217, 289, 626, 669
AhdI GACNNNNNGTC 1 cut(s) 238
AloI GAACNNNNNNTCC 2 cut(s) 462, 494
AluBI AGCT 2 cut(s) 57, 162
AluI AGCT 2 cut(s) 57, 162
Alw26I GTCTC 2 cut(s) 228, 465
AlwI GGATC 1 cut(s) 32
AlwNI CAGNNNCTG 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 566
AspS9I GGNCC 4 cut(s) 68, 149, 232, 326
AsuHPI GGTGA 3 cut(s) 19, 51, 583
AvaII GGWCC 4 cut(s) 68, 149, 232, 326
AxyI CCTNAGG 1 cut(s) 330
BbrPI CACGTG 1 cut(s) 167
BbvI GCAGC 1 cut(s) 553
BccI CCATC 1 cut(s) 554
BcoDI GTCTC 2 cut(s) 228, 465
BfaI CTAG 1 cut(s) 54
BisI GCNGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 568
Bme18I GGWCC 4 cut(s) 68, 149, 232, 326
BmeRI GACNNNNNGTC 1 cut(s) 238
BmgT120I GGNCC 4 cut(s) 68, 149, 232, 326
BmiI GGNNCC 2 cut(s) 233, 327
BmrI ACTGGG 2 cut(s) 204, 508
BmuI ACTGGG 2 cut(s) 204, 508
BsaAI YACGTR 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bse1I ACTGG 2 cut(s) 199, 503
Bse21I CCTNAGG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 1 cut(s) 204
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 199, 503
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 1 cut(s) 553
BseYI CCCAGC 1 cut(s) 30
BsgI GTGCAG 1 cut(s) 651
BslFI GGGAC 2 cut(s) 245, 390
BsmAI GTCTC 2 cut(s) 228, 465
BsmFI GGGAC 2 cut(s) 245, 390
Bsp1407I TGTACA 1 cut(s) 140
Bsp143I GATC 4 cut(s) 14, 24, 259, 577
BspACI CCGC 2 cut(s) 464, 569
BspCNI CTCAG 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 233, 327
BspPI GGATC 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 140
BsrI ACTGG 2 cut(s) 199, 503
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 4 cut(s) 14, 24, 259, 577
BssT1I CCWWGG 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 10, 427
Bst6I CTCTTC 1 cut(s) 397
BstAUI TGTACA 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 167
BstDEI CTNAG 2 cut(s) 129, 330
BstF5I GGATG 1 cut(s) 204
BstKTI GATC 4 cut(s) 17, 27, 262, 580
BstMAI GTCTC 2 cut(s) 228, 465
BstMBI GATC 4 cut(s) 14, 24, 259, 577
BstMWI GCNNNNNNNGC 2 cut(s) 45, 54
BstV1I GCAGC 1 cut(s) 553
BstXI CCANNNNNNTGG 1 cut(s) 313
Bsu36I CCTNAGG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 204
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 3 cut(s) 6, 24, 46
CaiI CAGNNNCTG 1 cut(s) 194
Cfr13I GGNCC 4 cut(s) 68, 149, 232, 326
Csp6I GTAC 2 cut(s) 141, 350
CviAII CATG 2 cut(s) 227, 608
CviJI RGCY 3 cut(s) 57, 162, 305
CviKI_1 RGCY 3 cut(s) 57, 162, 305
CviQI GTAC 2 cut(s) 141, 350
DdeI CTNAG 2 cut(s) 129, 330
DpnI GATC 4 cut(s) 16, 26, 261, 579
DpnII GATC 4 cut(s) 14, 24, 259, 577
DriI GACNNNNNGTC 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 397
Eam1105I GACNNNNNGTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 397
Eco130I CCWWGG 1 cut(s) 321
Eco32I GATATC 1 cut(s) 508
Eco47I GGWCC 4 cut(s) 68, 149, 232, 326
Eco72I CACGTG 1 cut(s) 167
Eco81I CCTNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 326
EcoRV GATATC 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 2 cut(s) 230, 611
FaqI GGGAC 2 cut(s) 245, 390
FatI CATG 2 cut(s) 226, 607
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 1 cut(s) 211
Fsp4HI GCNGC 1 cut(s) 567
FspBI CTAG 1 cut(s) 54
GluI GCNGC 1 cut(s) 567
GsaI CCCAGC 1 cut(s) 34
Hin1II CATG 2 cut(s) 230, 611
HinfI GANTC 1 cut(s) 635
HphI GGTGA 3 cut(s) 19, 51, 583
Hpy166II GTNNAC 3 cut(s) 6, 436, 523
Hpy188III TCNNGA 2 cut(s) 329, 650
Hpy8I GTNNAC 3 cut(s) 6, 436, 523
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 3 cut(s) 38, 93, 162
HpyCH4III ACNGT 2 cut(s) 10, 427
HpyCH4IV ACGT 2 cut(s) 166, 239
HpyCH4V TGCA 4 cut(s) 110, 188, 599, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 54
HpyF3I CTNAG 2 cut(s) 129, 330
HpySE526I ACGT 2 cut(s) 166, 239
Hsp92II CATG 2 cut(s) 230, 611
Kzo9I GATC 4 cut(s) 14, 24, 259, 577
LpnPI CCDG 8 cut(s) 44, 80, 180, 260, 299, 342, 467, 484
Lsp1109I GCAGC 1 cut(s) 553
MaeI CTAG 1 cut(s) 54
MaeII ACGT 2 cut(s) 166, 239
MalI GATC 4 cut(s) 16, 26, 261, 579
MboI GATC 4 cut(s) 14, 24, 259, 577
MboII GAAGA 3 cut(s) 132, 414, 515
MfeI CAATTG 1 cut(s) 144
MluCI AATT 5 cut(s) 144, 284, 360, 396, 621
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 4 cut(s) 15, 325, 367, 398
MseI TTAA 1 cut(s) 387
MunI CAATTG 1 cut(s) 144
MwoI GCNNNNNNNGC 2 cut(s) 45, 54
NdeII GATC 4 cut(s) 14, 24, 259, 577
NlaIII CATG 2 cut(s) 230, 611
NlaIV GGNNCC 2 cut(s) 233, 327
PkrI GCNGC 1 cut(s) 568
PleI GAGTC 1 cut(s) 629
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 1 cut(s) 629
Ppu21I YACGTR 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 326
Psp5II RGGWCCY 1 cut(s) 326
PspCI CACGTG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 233, 327
PspPI GGNCC 4 cut(s) 68, 149, 232, 326
PspPPI RGGWCCY 1 cut(s) 326
PstNI CAGNNNCTG 1 cut(s) 194
RsaI GTAC 2 cut(s) 142, 351
RsaNI GTAC 2 cut(s) 141, 350
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 4 cut(s) 14, 24, 259, 577
Sau96I GGNCC 4 cut(s) 68, 149, 232, 326
SchI GAGTC 1 cut(s) 629
SetI ASST 7 cut(s) 59, 164, 169, 242, 300, 336, 592
SinI GGWCC 4 cut(s) 68, 149, 232, 326
Sse9I AATT 5 cut(s) 144, 284, 360, 396, 621
SsiI CCGC 2 cut(s) 464, 569
SspMI CTAG 1 cut(s) 54
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 2 cut(s) 10, 427
TaiI ACGT 2 cut(s) 169, 242
TaqI TCGA 1 cut(s) 118
TasI AATT 5 cut(s) 144, 284, 360, 396, 621
TatI WGTACW 1 cut(s) 140
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 3 cut(s) 13, 24, 46
TseI GCWGC 1 cut(s) 566
TspDTI ATGAA 3 cut(s) 215, 504, 596
TspRI CASTG 3 cut(s) 13, 24, 46
VpaK11BI GGWCC 4 cut(s) 68, 149, 232, 326
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.