AT1G32440

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001739
Physical Location & Seq
Forward (+)
11711944 .. 11715162
3219 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32440.1

Sequence Viewer

Length: 1716 bp
ATGGCTGCTTATGGTCAAATCTCCTCGGGAATGACTGTAGATCCTCAGGTTCTCTCTTCCTCCAGAAACATTGGAGTTTCCCTATCACCTCTCCGGAGAACACTAATCGGCGCCGGAGTTAGGTCTACTAGTATCTCTCTCCGTCAATGTTCTCTCTCCGTTAGATCGATTAAAATCTCCGAAGATAGCCGCAAACCTAAAGCTTATGCAGAGAACGGTGCTTTTGATGTGGGAGTTTTGGATTCTTCATCATATAGATTGGCTGATTCAAGAACAAGTAGTAATGATTCAAGGAGGAAGACTAAGATTGTGTGTACGATTGGACCGTCTTCGAGTTCTAGGGAAATGATTTGGAAACTCGCGGAAGCTGGAATGAATGTGGCTCGTTTGAATATGTCTCATGGTGATCATGCTTCTCATCAGATAACTATTGATTTAGTTAAGGAGTATAATTCTTTGTTTGTTGACAAAGCTATTGCTATTATGTTGGATACAAAGGGTCCTGAGGTTCGAAGCGGGGATGTACCGCAGCCGATATTTCTTGAAGAGGGTCAAGAGTTTAACTTTACTATCAAGAGAGGTGTTTCGCTTAAAGACACTGTTAGTGTAAATTATGATGATTTTGTGAACGATGTTGAAGTTGGGGATATACTTTTGGTGGATGGTGGAATGATGTCGTTAGCTGTTAAATCAAAGACGAGTGATTTGGTGAAGTGTGTGGTTATTGATGGTGGAGAGCTTCAATCTAGACGTCACTTGAATGTTCGAGGAAAGAGTGCGACTCTTCCATCCATTACAGACAAAGATTGGGAAGACATAAAATTTGGAGTGGACAACCAAGTCGATTTCTACGCCGTCTCCTTTGTTAAGGATGCTAAAGTTGTCCATGAGTTGAAGAACTATCTCAAAACCTGCAGTGCAGACATATCGGTGATTGTGAAAATTGAAAGTGCAGACTCTATAAAGAATCTTCCTTCTATCATATCTGCTTGTGATGGGGCAATGGTTGCTCGTGGAGATCTTGGAGCTGAACTTCCCATTGAAGAGGTCCCGTTGTTACAGGAAGAAATAATCAGAAGGTGTAGAAGCATTCATAAACCAGTGATTGTTGCCACAAACATGCTAGAGAGTATGATTAATCATCCAACGCCTACAAGAGCTGAAGTCTCTGACATTGCAATTGCAGTACGTGAAGGCGCAGATGCTATCATGCTTTCTGGTGAAACCGCACATGGAAAGTTTCCGCTGAAAGCTGTTAACGTAATGCATACTGTGGCGTTGAGAACCGAGGCAAGTCTACCTGTCAGAACCTCGGCATCCCGTACCACTGCTTACAAGGGTCACATGGGCCAAATGTTTGCTTTTCATGCTTCTATAATGGCAAATACACTGAGCTCACCGCTAATTGTATTTACGAGAACCGGATCCATGGCAGTGCTTCTAAGCCACTACCGCCCATCTGCAACAATTTTCGCCTTCACAAACCAGAGAAGAATAATGCAAAGGCTTGCTCTTTATCAAGGTGTCATGCCTATATATATGGAGTTCTCGGATGATGCAGAAGATACATATGCCCGGTCTCTCAAACTCTTACAGGACGAGAATATGCTCAAGGAAGGACAACATGTAACTCTTGTCCAAAGTGGCTCGCAACCCATTTGGCGTGAAGAATCAACACATCTCATACAAGTCCGTAAGATAAAGATAGGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

571

Amino Acids

62.61

Weight (kDa)

7.68

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 99 - 419 1.2e-108 Pyruvate kinase, barrel domain
PK_C PF02887 466 - 548 1.4e-16 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 839
AatII GACGTC 1 cut(s) 754
Acc36I ACCTGC 1 cut(s) 920
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 2 cut(s) 125, 1297
AccII CGCG 1 cut(s) 362
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 8 cut(s) 190, 362, 516, 527, 1227, 1244, 1400, 1453
AclWI GGATC 3 cut(s) 35, 1419, 1432
AcsI RAATTY 1 cut(s) 821
AcuI CTGAAG 1 cut(s) 1182
AcyI GRCGYC 2 cut(s) 111, 751
AfaI GTAC 4 cut(s) 316, 525, 1188, 1324
AfiI CCNNNNNNNGG 1 cut(s) 120
AflIII ACRYGT 1 cut(s) 1624
AhlI ACTAGT 1 cut(s) 128
AluBI AGCT 9 cut(s) 203, 368, 473, 683, 739, 1028, 1160, 1253, 1395
AluI AGCT 9 cut(s) 203, 368, 473, 683, 739, 1028, 1160, 1253, 1395
Alw21I GWGCWC 1 cut(s) 1397
Alw26I GTCTC 4 cut(s) 402, 862, 1171, 1584
AlwI GGATC 3 cut(s) 35, 1419, 1432
Ama87I CYCGRG 1 cut(s) 25
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 1 cut(s) 1348
ApeKI GCWGC 2 cut(s) 5, 529
ApoI RAATTY 1 cut(s) 821
ArsI GACNNNNNNTTYG 4 cut(s) 688, 720, 806, 838
AseI ATTAAT 1 cut(s) 1137
AspLEI GCGC 2 cut(s) 113, 1199
AspS9I GGNCC 4 cut(s) 323, 500, 1048, 1348
AsuC2I CCSGG 1 cut(s) 1576
AsuHPI GGTGA 6 cut(s) 78, 416, 721, 943, 1232, 1389
AsuII TTCGAA 1 cut(s) 511
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 3 cut(s) 323, 500, 1048
AxyI CCTNAGG 2 cut(s) 45, 504
BamHI GGATCC 1 cut(s) 1424
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 1397
BauI CACGAG 1 cut(s) 1011
BbsI GAAGAC 3 cut(s) 305, 321, 819
Bbv12I GWGCWC 1 cut(s) 1397
BbvI GCAGC 1 cut(s) 541
BccI CCATC 5 cut(s) 656, 722, 796, 989, 1465
BceAI ACGGC 1 cut(s) 839
BcgI CGANNNNNNTGC 2 cut(s) 909, 943
BciVI GTATCC 1 cut(s) 484
BclI TGATCA 1 cut(s) 406
BcnI CCSGG 1 cut(s) 1576
BcoDI GTCTC 4 cut(s) 402, 862, 1171, 1584
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 4 cut(s) 129, 339, 747, 1124
BfmI CTRYAG 2 cut(s) 36, 913
BfoI RGCGCY 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 920
BfuI GTATCC 1 cut(s) 484
BglII AGATCT 1 cut(s) 1018
BisI GCNGC 3 cut(s) 6, 190, 530
BlsI GCNGC 3 cut(s) 7, 191, 531
Bme1390I CCNGG 1 cut(s) 1576
Bme18I GGWCC 3 cut(s) 323, 500, 1048
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 323, 500, 1048, 1348
BmiI GGNNCC 4 cut(s) 112, 501, 1050, 1426
BmrFI CCNGG 1 cut(s) 1576
BmsI GCATC 4 cut(s) 862, 1192, 1325, 1546
BpiI GAAGAC 3 cut(s) 305, 321, 819
BplI GAGNNNNNCTC 2 cut(s) 766, 798
BpmI CTGGAG 1 cut(s) 46
Bpu14I TTCGAA 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 1595
BpuMI CCSGG 1 cut(s) 1576
Bsa29I ATCGAT 1 cut(s) 167
BsaAI YACGTR 1 cut(s) 1190
BsaBI GATNNNNATC 1 cut(s) 173
BsaHI GRCGYC 2 cut(s) 111, 751
BsaI GGTCTC 1 cut(s) 1584
BsaJI CCNNGG 4 cut(s) 24, 1287, 1311, 1428
BsaWI WCCGGW 2 cut(s) 93, 1421
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 1 cut(s) 1100
Bse21I CCTNAGG 2 cut(s) 45, 504
Bse3DI GCAATG 2 cut(s) 1008, 1173
Bse8I GATNNNNATC 1 cut(s) 173
BseAI TCCGGA 1 cut(s) 93
BseCI ATCGAT 1 cut(s) 167
BseDI CCNNGG 4 cut(s) 24, 1287, 1311, 1428
BseGI GGATG 7 cut(s) 526, 667, 788, 877, 1141, 1316, 1558
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 2 cut(s) 1008, 1173
BseMII CTCAG 3 cut(s) 59, 495, 1382
BseNI ACTGG 1 cut(s) 1100
BseRI GAGGAG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 541
BsgI GTGCAG 2 cut(s) 939, 972
Bsh1236I CGCG 1 cut(s) 362
BshFI GGCC 1 cut(s) 1350
BshNI GGYRCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 1397
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 4 cut(s) 94, 114, 1422, 1576
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 4 cut(s) 402, 862, 1171, 1584
BsmBI CGTCTC 1 cut(s) 862
BsmFI GGGAC 1 cut(s) 1034
BsmI GAATGC 1 cut(s) 1089
BsnI GGCC 1 cut(s) 1350
Bso31I GGTCTC 1 cut(s) 1584
BsoBI CYCGRG 1 cut(s) 25
Bsp119I TTCGAA 1 cut(s) 511
Bsp1286I GDGCHC 1 cut(s) 1397
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 5 cut(s) 40, 164, 406, 1018, 1424
Bsp19I CCATGG 1 cut(s) 1428
BspACI CCGC 8 cut(s) 190, 362, 516, 527, 1227, 1244, 1400, 1453
BspANI GGCC 1 cut(s) 1350
BspCNI CTCAG 3 cut(s) 58, 496, 1383
BspDI ATCGAT 1 cut(s) 167
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 4 cut(s) 112, 501, 1050, 1426
BspMAI CTGCAG 1 cut(s) 917
BspMI ACCTGC 1 cut(s) 920
BspPI GGATC 3 cut(s) 35, 1419, 1432
BspT104I TTCGAA 1 cut(s) 511
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 1584
BsrDI GCAATG 2 cut(s) 1008, 1173
BsrI ACTGG 1 cut(s) 1100
BssECI CCNNGG 4 cut(s) 24, 1287, 1311, 1428
BssMI GATC 5 cut(s) 40, 164, 406, 1018, 1424
BssNI GRCGYC 2 cut(s) 111, 751
BssSI CACGAG 1 cut(s) 1011
BssT1I CCWWGG 1 cut(s) 1428
Bst2BI CACGAG 1 cut(s) 1011
Bst4CI ACNGT 5 cut(s) 37, 218, 327, 601, 1273
Bst6I CTCTTC 4 cut(s) 61, 540, 789, 1038
BstACI GRCGYC 2 cut(s) 111, 751
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstBAI YACGTR 1 cut(s) 1190
BstBI TTCGAA 1 cut(s) 511
BstC8I GCNNGC 2 cut(s) 1509, 1649
BstDEI CTNAG 5 cut(s) 45, 303, 504, 1391, 1442
BstDSI CCRYGG 1 cut(s) 1428
BstF5I GGATG 7 cut(s) 526, 667, 788, 877, 1141, 1316, 1558
BstFNI CGCG 1 cut(s) 362
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 2 cut(s) 113, 1199
BstKTI GATC 5 cut(s) 43, 167, 409, 1021, 1427
BstMAI GTCTC 4 cut(s) 402, 862, 1171, 1584
BstMBI GATC 5 cut(s) 40, 164, 406, 1018, 1424
BstMWI GCNNNNNNNGC 3 cut(s) 1007, 1367, 1452
BstNSI RCATGY 2 cut(s) 1123, 1628
BstSCI CCNGG 1 cut(s) 1574
BstSFI CTRYAG 2 cut(s) 36, 913
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 1 cut(s) 541
BstV2I GAAGAC 3 cut(s) 305, 321, 819
BstX2I RGATCY 3 cut(s) 40, 1018, 1424
BstYI RGATCY 3 cut(s) 40, 1018, 1424
Bsu15I ATCGAT 1 cut(s) 167
Bsu36I CCTNAGG 2 cut(s) 45, 504
BsuI GTATCC 1 cut(s) 484
BsuRI GGCC 1 cut(s) 1350
BsuTUI ATCGAT 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 1428
BtsCI GGATG 7 cut(s) 526, 667, 788, 877, 1141, 1316, 1558
BtsI GCAGTG 3 cut(s) 922, 1326, 1440
BtsIMutI CAGTG 6 cut(s) 597, 922, 1107, 1326, 1388, 1440
BveI ACCTGC 1 cut(s) 920
Cac8I GCNNGC 2 cut(s) 1509, 1649
CfoI GCGC 2 cut(s) 113, 1199
Cfr13I GGNCC 4 cut(s) 323, 500, 1048, 1348
ClaI ATCGAT 1 cut(s) 167
Csp6I GTAC 4 cut(s) 315, 524, 1187, 1323
CviQI GTAC 4 cut(s) 315, 524, 1187, 1323
DdeI CTNAG 5 cut(s) 45, 303, 504, 1391, 1442
DinI GGCGCC 1 cut(s) 112
DpnI GATC 5 cut(s) 42, 166, 408, 1020, 1426
DpnII GATC 5 cut(s) 40, 164, 406, 1018, 1424
DrdI GACNNNNNNGTC 1 cut(s) 839
DseDI GACNNNNNNGTC 1 cut(s) 839
Eam1104I CTCTTC 4 cut(s) 61, 540, 789, 1038
EarI CTCTTC 4 cut(s) 61, 540, 789, 1038
Ecl136II GAGCTC 1 cut(s) 1395
Eco130I CCWWGG 1 cut(s) 1428
Eco24I GRGCYC 1 cut(s) 1397
Eco31I GGTCTC 1 cut(s) 1584
Eco47I GGWCC 3 cut(s) 323, 500, 1048
Eco53kI GAGCTC 1 cut(s) 1395
Eco57I CTGAAG 1 cut(s) 1182
Eco81I CCTNAGG 2 cut(s) 45, 504
Eco88I CYCGRG 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 1395
EcoO109I RGGNCCY 2 cut(s) 500, 1048
EcoT14I CCWWGG 1 cut(s) 1428
EcoT22I ATGCAT 1 cut(s) 1269
EcoT38I GRGCYC 1 cut(s) 1397
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 1428
Esp3I CGTCTC 1 cut(s) 862
FaqI GGGAC 1 cut(s) 1034
FauI CCCGC 1 cut(s) 509
FauNDI CATATG 1 cut(s) 1570
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 2 cut(s) 125, 1297
Fnu4HI GCNGC 3 cut(s) 6, 190, 530
FokI GGATG 7 cut(s) 533, 674, 775, 884, 1128, 1303, 1565
FriOI GRGCYC 1 cut(s) 1397
Fsp4HI GCNGC 3 cut(s) 6, 190, 530
FspBI CTAG 4 cut(s) 129, 339, 747, 1124
GlaI GCGC 2 cut(s) 112, 1198
GluI GCNGC 3 cut(s) 6, 190, 530
GsuI CTGGAG 1 cut(s) 46
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 1 cut(s) 1350
HapII CCGG 4 cut(s) 94, 114, 1422, 1576
HhaI GCGC 2 cut(s) 113, 1199
Hin1I GRCGYC 2 cut(s) 111, 751
Hin6I GCGC 2 cut(s) 111, 1197
HinP1I GCGC 2 cut(s) 111, 1197
HincII GTYRAC 2 cut(s) 466, 1258
HindII GTYRAC 2 cut(s) 466, 1258
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 7 cut(s) 242, 266, 287, 781, 956, 967, 1670
HpaI GTTAAC 1 cut(s) 1258
HpaII CCGG 4 cut(s) 94, 114, 1422, 1576
HphI GGTGA 6 cut(s) 78, 416, 721, 943, 1232, 1389
Hpy166II GTNNAC 7 cut(s) 126, 315, 466, 628, 832, 1258, 1298
Hpy188I TCNGA 6 cut(s) 181, 423, 1076, 1171, 1307, 1552
Hpy188III TCNNGA 9 cut(s) 27, 63, 94, 270, 503, 542, 554, 574, 747
Hpy8I GTNNAC 7 cut(s) 126, 315, 466, 628, 832, 1258, 1298
HpyAV CCTTC 5 cut(s) 984, 1071, 1187, 1486, 1610
HpyCH4III ACNGT 5 cut(s) 37, 218, 327, 601, 1273
HpyCH4IV ACGT 3 cut(s) 751, 1189, 1260
HpyF10VI GCNNNNNNNGC 3 cut(s) 1007, 1367, 1452
HpyF3I CTNAG 5 cut(s) 45, 303, 504, 1391, 1442
HpySE526I ACGT 3 cut(s) 751, 1189, 1260
Hsp92I GRCGYC 2 cut(s) 111, 751
HspAI GCGC 2 cut(s) 111, 1197
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 406
KspAI GTTAAC 1 cut(s) 1258
Kzo9I GATC 5 cut(s) 40, 164, 406, 1018, 1424
LmnI GCTCC 1 cut(s) 1025
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 4 cut(s) 862, 1192, 1325, 1546
MaeI CTAG 4 cut(s) 129, 339, 747, 1124
MaeII ACGT 3 cut(s) 751, 1189, 1260
MaeIII GTNAC 4 cut(s) 752, 1056, 1340, 1627
MalI GATC 5 cut(s) 42, 166, 408, 1020, 1426
MboI GATC 5 cut(s) 40, 164, 406, 1018, 1424
MfeI CAATTG 1 cut(s) 1179
MflI RGATCY 3 cut(s) 40, 1018, 1424
MhlI GDGCHC 1 cut(s) 1397
MluCI AATT 7 cut(s) 451, 610, 821, 942, 1179, 1404, 1467
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 2 cut(s) 775, 950
MmeI TCCRAC 2 cut(s) 468, 1169
Mph1103I ATGCAT 1 cut(s) 1269
MroI TCCGGA 1 cut(s) 93
MseI TTAA 8 cut(s) 171, 441, 561, 591, 687, 867, 1137, 1257
MslI CAYNNNNRTG 4 cut(s) 759, 929, 1118, 1433
MspA1I CMGCKG 1 cut(s) 1246
MspI CCGG 4 cut(s) 94, 114, 1422, 1576
MspR9I CCNGG 1 cut(s) 1576
MunI CAATTG 1 cut(s) 1179
Mva1269I GAATGC 1 cut(s) 1089
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 3 cut(s) 1007, 1367, 1452
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 1576
NcoI CCATGG 1 cut(s) 1428
NdeI CATATG 1 cut(s) 1570
NdeII GATC 5 cut(s) 40, 164, 406, 1018, 1424
NlaIV GGNNCC 4 cut(s) 112, 501, 1050, 1426
NmeAIII GCCGAG 1 cut(s) 1292
NmuCI GTSAC 2 cut(s) 752, 1340
NsiI ATGCAT 1 cut(s) 1269
NspI RCATGY 2 cut(s) 1123, 1628
NspV TTCGAA 1 cut(s) 511
PciI ACATGT 1 cut(s) 1624
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PctI GAATGC 1 cut(s) 1089
PfeI GAWTC 5 cut(s) 242, 266, 287, 967, 1670
PkrI GCNGC 3 cut(s) 7, 191, 531
PleI GAGTC 2 cut(s) 775, 950
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 2 cut(s) 775, 950
Ppu21I YACGTR 1 cut(s) 1190
PpuMI RGGWCCY 2 cut(s) 500, 1048
PscI ACATGT 1 cut(s) 1624
PshBI ATTAAT 1 cut(s) 1137
Psp124BI GAGCTC 1 cut(s) 1397
Psp5II RGGWCCY 2 cut(s) 500, 1048
PspN4I GGNNCC 4 cut(s) 112, 501, 1050, 1426
PspPI GGNCC 4 cut(s) 323, 500, 1048, 1348
PspPPI RGGWCCY 2 cut(s) 500, 1048
PstI CTGCAG 1 cut(s) 917
PsuI RGATCY 3 cut(s) 40, 1018, 1424
RsaI GTAC 4 cut(s) 316, 525, 1188, 1324
RsaNI GTAC 4 cut(s) 315, 524, 1187, 1323
RseI CAYNNNNRTG 4 cut(s) 759, 929, 1118, 1433
SacI GAGCTC 1 cut(s) 1397
SaqAI TTAA 8 cut(s) 171, 441, 561, 591, 687, 867, 1137, 1257
SatI GCNGC 3 cut(s) 6, 190, 530
Sau3AI GATC 5 cut(s) 40, 164, 406, 1018, 1424
Sau96I GGNCC 4 cut(s) 323, 500, 1048, 1348
SchI GAGTC 2 cut(s) 775, 950
ScrFI CCNGG 1 cut(s) 1576
SduI GDGCHC 1 cut(s) 1397
SfaNI GCATC 4 cut(s) 862, 1192, 1325, 1546
SfcI CTRYAG 2 cut(s) 36, 913
SfoI GGCGCC 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 511
SinI GGWCC 3 cut(s) 323, 500, 1048
SmiMI CAYNNNNRTG 4 cut(s) 759, 929, 1118, 1433
SmlI CTYRAG 1 cut(s) 1610
SmoI CTYRAG 1 cut(s) 1610
SpeI ACTAGT 1 cut(s) 128
Sse9I AATT 7 cut(s) 451, 610, 821, 942, 1179, 1404, 1467
SsiI CCGC 8 cut(s) 190, 362, 516, 527, 1227, 1244, 1400, 1453
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 4 cut(s) 129, 339, 747, 1124
SstI GAGCTC 1 cut(s) 1397
StyD4I CCNGG 1 cut(s) 1574
StyI CCWWGG 1 cut(s) 1428
TaaI ACNGT 5 cut(s) 37, 218, 327, 601, 1273
TaiI ACGT 3 cut(s) 754, 1192, 1263
TaqI TCGA 5 cut(s) 167, 332, 511, 766, 843
TasI AATT 7 cut(s) 451, 610, 821, 942, 1179, 1404, 1467
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 5 cut(s) 242, 266, 287, 967, 1670
Tru1I TTAA 8 cut(s) 171, 441, 561, 591, 687, 867, 1137, 1257
Tru9I TTAA 8 cut(s) 171, 441, 561, 591, 687, 867, 1137, 1257
TscAI CASTG 6 cut(s) 604, 922, 1107, 1333, 1395, 1440
TseFI GTSAC 2 cut(s) 752, 1340
TseI GCWGC 2 cut(s) 5, 529
Tsp45I GTSAC 2 cut(s) 752, 1340
TspDTI ATGAA 4 cut(s) 237, 389, 1082, 1355
TspGWI ACGGA 3 cut(s) 131, 148, 1682
TspRI CASTG 6 cut(s) 604, 922, 1107, 1333, 1395, 1440
VpaK11BI GGWCC 3 cut(s) 323, 500, 1048
VspI ATTAAT 1 cut(s) 1137
XapI RAATTY 1 cut(s) 821
XbaI TCTAGA 1 cut(s) 746
XceI RCATGY 2 cut(s) 1123, 1628
XmiI GTMKAC 2 cut(s) 125, 1297
XspI CTAG 4 cut(s) 129, 339, 747, 1124
ZraI GACGTC 1 cut(s) 752
Zsp2I ATGCAT 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.