MD06G1029300.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001739
Physical Location & Seq
Forward (+)
3497545 .. 3504351
6807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1029300.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGGCGACTATTAATGTTCCGGCGAGAATGTATGTCTTGAAACCTGGCAAGACTAAGGCCGCTGATCGATTAGCTTCCTCCAAGTGCCTGACTGGTTCCGTTGGGTTCGACTGCCGACCCAGAAGAAAATATGCCCACAACCATAACCACAAGAACCACAAGCAATTTTGTACTGTTAAATCAATTGGGATCACCCAGGACAGCAGCAGAGGCCATGTCAACGGTCCTCTTGGTGGCGTTGGGAATCTGGACTCAACTTTTGAACTTCAGTCTGATGAGTATGTTGTGGGCCAGCCGAAGGTATTTTCCGATGGTCAAAGAAAAACTAAGATAGTATGTACGATTGGTCCTTCGACAAGCTCACGTGAAATGATATGGAAACTGGCAGAAACTGGAATGAACGTCGCACGTTTGAATATGTCACATGGAGACCATGCATCTCATCAGAAAACCATTGATATTGTCAAAGAATACAATTCTCAATTTGAAGACAAGGTTGTAGCAATAATGCTGGACACCAAGGGTCCTGAGGTTAGGAGTGGAGATGTACCACAGCCGATTCTACTTAAAGAAGGACAAGAGTTTAACTTCACAATTAAAAGGGGAGTTAGCACTGCAGACACTGTTAGCGTAAACTATGACGACTTTGTTAACGACGTGGAGGTTGGTGACATTATTCTAGTTGATGGTGGAATGATGTCTTTAGCTGTAAAGTCAAAGACAAAAGATGTGGTTAAATGTGTGGTAGTTGATGGTGGAGAACTAAAATCAAGGCGCCATCTAAACGTGCGTGGAAAAAGTGCAACTCTGCCTTCAATAACAGAAAAGGACTGGGAAGATATCAAGTTTGGAGTGGACAATGGAGTTGATTTCTATGCTGTATCATTTGTAAAAAATGCTGCAGTGGTACACGAGTTGAAAGATTATCTCAAAGGTTGCAATGCAGATATTCATGTTATTGTAAAAATAGAAAGCGCGGACTCTATACCAAATCTTCATTCAATAATTTCTGCATCTGATGGGGCGATGATTGCTCGTGGAGACCTTGGAGCTGAGCTGCCAATTGAGGAAGTTCCTCTGTTGCAGGAGGACATCATTAGAAGGTGTCGCAATGTGCAGAAACCAGTGATTGTAGCAACAAACATGCTGGAGAGCATGATTGATCATCCTACGCCGACCAGGGCAGAAGTCTCTGACATTGCAATTGCAGTCAGAGCAGGTGCTGATGCAATCATGCTTTCTGGAGAAACTGCCCATGGAAAGTATCCATTGAAGGCTGTCAAAGTTATGCATACTGTAGCATTAAGGACTGAGTCAAGCCTACCAGTTAGCACTATTCCTCCAATACAACTGAGGACCTTTAAGAAACATATGGGGGAAATGTTTGCTTTCCATTCCACAACTATAGCAAACAGTCTCAATACACCCATCATTGTTTTCACAAGGACAGGTTCCATGGCTGTGCTTTTAAGCCATTATCGTCCTTGCTCAACGATCTTTGCCTTCACAAACGAAGAGAGAATTAAGCAGAGATTGGTTCTTTATCACGGTGTCCGCCCCATATATATGCAGTTCTCAAATGATGCGGAGGAGACTTTCTCAAGAGCAATAAAGCTGCTAGTGGCAAAGGATCTTTCGAAAGAGGGACAGTTTGTAACTCTCGTCCAAAGCGGTGCACAACCAATCTGGCGTAAAGAGTCTTCTCACCATATCCAAGTTCGTAAAGTTGAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

579

Amino Acids

63.7

Weight (kDa)

8.78

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 107 - 427 1e-111 Pyruvate kinase, barrel domain
PK_C PF02887 468 - 556 3.4e-16 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1208
Acc36I ACCTGC 1 cut(s) 1208
AccB1I GGYRCC 1 cut(s) 774
AccII CGCG 1 cut(s) 977
AciI CCGC 5 cut(s) 60, 977, 1555, 1586, 1671
AclWI GGATC 2 cut(s) 197, 1638
AcuI CTGAAG 1 cut(s) 251
AcvI CACGTG 1 cut(s) 365
AcyI GRCGYC 1 cut(s) 775
AfaI GTAC 4 cut(s) 172, 340, 549, 909
AfiI CCNNNNNNNGG 2 cut(s) 233, 298
AgsI TTSAA 9 cut(s) 40, 263, 415, 488, 816, 919, 1002, 1273, 1730
AjiI CACGTC 1 cut(s) 658
AjnI CCWGG 3 cut(s) 43, 195, 1178
AjuI GAANNNNNNNTTGG 2 cut(s) 982, 1014
AluBI AGCT 6 cut(s) 74, 360, 707, 1052, 1057, 1615
AluI AGCT 6 cut(s) 74, 360, 707, 1052, 1057, 1615
Alw21I GWGCWC 1 cut(s) 1678
Alw26I GTCTC 5 cut(s) 423, 1035, 1195, 1421, 1586
Alw44I GTGCAC 1 cut(s) 1674
AlwI GGATC 2 cut(s) 197, 1638
AlwNI CAGNNNCTG 3 cut(s) 392, 623, 1223
AoxI GGCC 3 cut(s) 57, 211, 289
ApaLI GTGCAC 1 cut(s) 1674
ApeKI GCWGC 4 cut(s) 204, 899, 1057, 1615
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AseI ATTAAT 1 cut(s) 12
AspLEI GCGC 2 cut(s) 777, 977
AspS9I GGNCC 5 cut(s) 224, 289, 347, 524, 1356
AsuHPI GGTGA 3 cut(s) 184, 680, 1697
AsuII TTCGAA 1 cut(s) 1637
AvaII GGWCC 4 cut(s) 224, 347, 524, 1356
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 1678
BaeI ACNNNNGTAYC 2 cut(s) 899, 932
BanI GGYRCC 1 cut(s) 774
BarI GAAGNNNNNNTAC 2 cut(s) 1684, 1716
BauI CACGAG 2 cut(s) 911, 1035
BbrPI CACGTG 1 cut(s) 365
BbsI GAAGAC 2 cut(s) 495, 1692
Bbv12I GWGCWC 1 cut(s) 1678
BbvI GCAGC 4 cut(s) 216, 886, 1044, 1602
BccI CCATC 6 cut(s) 305, 680, 746, 786, 1013, 1436
BciT130I CCWGG 3 cut(s) 45, 197, 1180
BciVI GTATCC 1 cut(s) 1275
BclI TGATCA 1 cut(s) 1162
BcoDI GTCTC 5 cut(s) 423, 1035, 1195, 1421, 1586
BfaI CTAG 2 cut(s) 680, 1619
BfmI CTRYAG 4 cut(s) 615, 900, 1296, 1404
BfoI RGCGCY 1 cut(s) 778
BfuAI ACCTGC 1 cut(s) 1208
BfuI GTATCC 1 cut(s) 1275
BisI GCNGC 5 cut(s) 60, 205, 900, 1058, 1616
BlpI GCTNAGC 1 cut(s) 1053
BlsI GCNGC 5 cut(s) 61, 206, 901, 1059, 1617
Bme1390I CCNGG 3 cut(s) 45, 197, 1180
Bme18I GGWCC 4 cut(s) 224, 347, 524, 1356
BmgBI CACGTC 1 cut(s) 658
BmgT120I GGNCC 5 cut(s) 224, 289, 347, 524, 1356
BmiI GGNNCC 4 cut(s) 97, 525, 776, 1453
BmrFI CCNGG 3 cut(s) 45, 197, 1180
BmrI ACTGGG 1 cut(s) 841
BmsI GCATC 4 cut(s) 446, 1022, 1216, 1573
BmuI ACTGGG 1 cut(s) 841
BpiI GAAGAC 2 cut(s) 495, 1692
BplI GAGNNNNNCTC 2 cut(s) 1583, 1615
BpmI CTGGAG 2 cut(s) 1169, 1263
Bpu1102I GCTNAGC 1 cut(s) 1053
Bpu14I TTCGAA 1 cut(s) 1637
BpuEI CTTGAG 1 cut(s) 1585
Bsa29I ATCGAT 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 365
BsaHI GRCGYC 1 cut(s) 775
BsaI GGTCTC 2 cut(s) 423, 1035
BsaJI CCNNGG 6 cut(s) 195, 519, 1045, 1179, 1255, 1455
BsaXI ACNNNNNCTCC 2 cut(s) 1324, 1354
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 298
Bse1I ACTGG 6 cut(s) 97, 387, 397, 836, 1124, 1325
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 946, 1117, 1197
BseBI CCWGG 3 cut(s) 45, 197, 1180
BseCI ATCGAT 1 cut(s) 67
BseDI CCNNGG 6 cut(s) 195, 519, 1045, 1179, 1255, 1455
BseGI GGATG 1 cut(s) 1165
BseLI CCNNNNNNNGG 2 cut(s) 233, 298
BseMI GCAATG 3 cut(s) 946, 1117, 1197
BseMII CTCAG 4 cut(s) 519, 1044, 1302, 1343
BseNI ACTGG 6 cut(s) 97, 387, 397, 836, 1124, 1325
BseRI GAGGAG 1 cut(s) 1604
BseSI GKGCMC 1 cut(s) 1678
BseXI GCAGC 4 cut(s) 216, 886, 1044, 1602
BsgI GTGCAG 1 cut(s) 1136
Bsh1236I CGCG 1 cut(s) 977
BshFI GGCC 3 cut(s) 59, 213, 291
BshNI GGYRCC 1 cut(s) 774
BshVI ATCGAT 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 1678
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 1659
BslI CCNNNNNNNGG 2 cut(s) 233, 298
BsmAI GTCTC 5 cut(s) 423, 1035, 1195, 1421, 1586
BsmFI GGGAC 1 cut(s) 1659
BsnI GGCC 3 cut(s) 59, 213, 291
Bso31I GGTCTC 2 cut(s) 423, 1035
Bsp119I TTCGAA 1 cut(s) 1637
Bsp1286I GDGCHC 1 cut(s) 1678
Bsp143I GATC 5 cut(s) 64, 189, 1162, 1494, 1630
Bsp1720I GCTNAGC 1 cut(s) 1053
Bsp19I CCATGG 2 cut(s) 1255, 1455
BspACI CCGC 5 cut(s) 60, 977, 1555, 1586, 1671
BspANI GGCC 3 cut(s) 59, 213, 291
BspCNI CTCAG 4 cut(s) 520, 1045, 1303, 1344
BspDI ATCGAT 1 cut(s) 67
BspFNI CGCG 1 cut(s) 977
BspLI GGNNCC 4 cut(s) 97, 525, 776, 1453
BspMAI CTGCAG 2 cut(s) 619, 904
BspMI ACCTGC 1 cut(s) 1208
BspPI GGATC 2 cut(s) 197, 1638
BspT104I TTCGAA 1 cut(s) 1637
BspT107I GGYRCC 1 cut(s) 774
BspTNI GGTCTC 2 cut(s) 423, 1035
BsrDI GCAATG 3 cut(s) 946, 1117, 1197
BsrI ACTGG 6 cut(s) 97, 387, 397, 836, 1124, 1325
BssECI CCNNGG 6 cut(s) 195, 519, 1045, 1179, 1255, 1455
BssMI GATC 5 cut(s) 64, 189, 1162, 1494, 1630
BssNI GRCGYC 1 cut(s) 775
BssSI CACGAG 2 cut(s) 911, 1035
BssT1I CCWWGG 4 cut(s) 519, 1045, 1255, 1455
Bst2BI CACGAG 2 cut(s) 911, 1035
Bst2UI CCWGG 3 cut(s) 45, 197, 1180
Bst4CI ACNGT 7 cut(s) 175, 224, 625, 1297, 1415, 1550, 1650
Bst6I CTCTTC 1 cut(s) 1509
BstACI GRCGYC 1 cut(s) 775
BstBAI YACGTR 1 cut(s) 365
BstBI TTCGAA 1 cut(s) 1637
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 6 cut(s) 54, 327, 528, 1053, 1311, 1352
BstDSI CCRYGG 2 cut(s) 1255, 1455
BstF5I GGATG 1 cut(s) 1165
BstFNI CGCG 1 cut(s) 977
BstH2I RGCGCY 1 cut(s) 778
BstHHI GCGC 2 cut(s) 777, 977
BstKTI GATC 5 cut(s) 67, 192, 1165, 1497, 1633
BstMAI GTCTC 5 cut(s) 423, 1035, 1195, 1421, 1586
BstMBI GATC 5 cut(s) 64, 189, 1162, 1494, 1630
BstMWI GCNNNNNNNGC 3 cut(s) 210, 1031, 1214
BstNI CCWGG 3 cut(s) 45, 197, 1180
BstNSI RCATGY 1 cut(s) 1147
BstSCI CCNGG 3 cut(s) 43, 195, 1178
BstSFI CTRYAG 4 cut(s) 615, 900, 1296, 1404
BstSLI GKGCMC 1 cut(s) 1678
BstUI CGCG 1 cut(s) 977
BstV1I GCAGC 4 cut(s) 216, 886, 1044, 1602
BstV2I GAAGAC 2 cut(s) 495, 1692
BstX2I RGATCY 1 cut(s) 1630
BstYI RGATCY 1 cut(s) 1630
Bsu15I ATCGAT 1 cut(s) 67
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 1275
BsuRI GGCC 3 cut(s) 59, 213, 291
BsuTUI ATCGAT 1 cut(s) 67
BtgI CCRYGG 2 cut(s) 1255, 1455
BtgZI GCGATG 1 cut(s) 1040
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 1 cut(s) 1165
BtsI GCAGTG 2 cut(s) 612, 909
BtsIMutI CAGTG 4 cut(s) 612, 621, 909, 1131
BveI ACCTGC 1 cut(s) 1208
Cac8I GCNNGC 1 cut(s) 293
CaiI CAGNNNCTG 3 cut(s) 392, 623, 1223
CfoI GCGC 2 cut(s) 777, 977
Cfr13I GGNCC 5 cut(s) 224, 289, 347, 524, 1356
ClaI ATCGAT 1 cut(s) 67
Csp6I GTAC 4 cut(s) 171, 339, 548, 908
CspCI CAANNNNNGTGG 2 cut(s) 711, 746
CviAII CATG 9 cut(s) 215, 425, 434, 953, 1144, 1156, 1234, 1256, 1456
CviQI GTAC 4 cut(s) 171, 339, 548, 908
DdeI CTNAG 6 cut(s) 54, 327, 528, 1053, 1311, 1352
DinI GGCGCC 1 cut(s) 776
DpnI GATC 5 cut(s) 66, 191, 1164, 1496, 1632
DpnII GATC 5 cut(s) 64, 189, 1162, 1494, 1630
Eam1104I CTCTTC 1 cut(s) 1509
EarI CTCTTC 1 cut(s) 1509
EciI GGCGGA 1 cut(s) 1544
Eco130I CCWWGG 4 cut(s) 519, 1045, 1255, 1455
Eco31I GGTCTC 2 cut(s) 423, 1035
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 4 cut(s) 224, 347, 524, 1356
Eco57I CTGAAG 1 cut(s) 251
Eco72I CACGTG 1 cut(s) 365
Eco81I CCTNAGG 1 cut(s) 528
EcoO109I RGGNCCY 2 cut(s) 524, 1356
EcoRII CCWGG 3 cut(s) 43, 195, 1178
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 4 cut(s) 519, 1045, 1255, 1455
EcoT22I ATGCAT 2 cut(s) 439, 1293
EgeI GGCGCC 1 cut(s) 776
EheI GGCGCC 1 cut(s) 776
ErhI CCWWGG 4 cut(s) 519, 1045, 1255, 1455
FaeI CATG 9 cut(s) 218, 428, 437, 956, 1147, 1159, 1237, 1259, 1459
FaqI GGGAC 1 cut(s) 1659
FatI CATG 9 cut(s) 214, 424, 433, 952, 1143, 1155, 1233, 1255, 1455
FauNDI CATATG 1 cut(s) 1371
FbaI TGATCA 1 cut(s) 1162
Fnu4HI GCNGC 5 cut(s) 60, 205, 900, 1058, 1616
FokI GGATG 1 cut(s) 1152
Fsp4HI GCNGC 5 cut(s) 60, 205, 900, 1058, 1616
FspBI CTAG 2 cut(s) 680, 1619
GlaI GCGC 2 cut(s) 776, 976
GluI GCNGC 5 cut(s) 60, 205, 900, 1058, 1616
GsuI CTGGAG 2 cut(s) 1169, 1263
HaeII RGCGCY 1 cut(s) 778
HaeIII GGCC 3 cut(s) 59, 213, 291
HapII CCGG 1 cut(s) 20
HhaI GCGC 2 cut(s) 777, 977
Hin1I GRCGYC 1 cut(s) 775
Hin1II CATG 9 cut(s) 218, 428, 437, 956, 1147, 1159, 1237, 1259, 1459
Hin6I GCGC 2 cut(s) 775, 975
HinP1I GCGC 2 cut(s) 775, 975
HincII GTYRAC 2 cut(s) 220, 652
HindII GTYRAC 2 cut(s) 220, 652
HinfI GANTC 6 cut(s) 244, 251, 559, 980, 1313, 1697
HpaI GTTAAC 1 cut(s) 652
HpaII CCGG 1 cut(s) 20
HphI GGTGA 3 cut(s) 184, 680, 1697
Hpy166II GTNNAC 6 cut(s) 220, 634, 652, 856, 910, 1676
Hpy188I TCNGA 6 cut(s) 274, 310, 447, 1018, 1195, 1214
Hpy188III TCNNGA 5 cut(s) 37, 248, 527, 1242, 1602
Hpy8I GTNNAC 6 cut(s) 220, 634, 652, 856, 910, 1676
Hpy99I CGWCG 2 cut(s) 407, 659
HpyAV CCTTC 8 cut(s) 292, 360, 566, 822, 1095, 1267, 1513, 1724
HpyCH4III ACNGT 7 cut(s) 175, 224, 625, 1297, 1415, 1550, 1650
HpyCH4IV ACGT 5 cut(s) 364, 402, 409, 657, 786
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 1031, 1214
HpyF3I CTNAG 6 cut(s) 54, 327, 528, 1053, 1311, 1352
HpySE526I ACGT 5 cut(s) 364, 402, 409, 657, 786
Hsp92I GRCGYC 1 cut(s) 775
Hsp92II CATG 9 cut(s) 218, 428, 437, 956, 1147, 1159, 1237, 1259, 1459
HspAI GCGC 2 cut(s) 775, 975
KasI GGCGCC 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 1162
KspAI GTTAAC 1 cut(s) 652
Kzo9I GATC 5 cut(s) 64, 189, 1162, 1494, 1630
LmnI GCTCC 1 cut(s) 1049
Lsp1109I GCAGC 4 cut(s) 216, 886, 1044, 1602
LweI GCATC 4 cut(s) 446, 1022, 1216, 1573
MaeI CTAG 2 cut(s) 680, 1619
MaeII ACGT 5 cut(s) 364, 402, 409, 657, 786
MaeIII GTNAC 3 cut(s) 420, 668, 1654
MalI GATC 5 cut(s) 66, 191, 1164, 1496, 1632
MboI GATC 5 cut(s) 64, 189, 1162, 1494, 1630
MboII GAAGA 6 cut(s) 135, 500, 848, 986, 1526, 1692
MfeI CAATTG 3 cut(s) 183, 1062, 1203
MflI RGATCY 1 cut(s) 1630
MhlI GDGCHC 1 cut(s) 1678
MluCI AATT 9 cut(s) 164, 183, 475, 482, 594, 1005, 1062, 1203, 1521
Mly113I GGCGCC 1 cut(s) 775
MlyI GAGTC 4 cut(s) 245, 974, 1322, 1706
Mph1103I ATGCAT 2 cut(s) 439, 1293
MslI CAYNNNNRTG 2 cut(s) 1460, 1565
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 3 cut(s) 45, 197, 1180
MunI CAATTG 3 cut(s) 183, 1062, 1203
MvaI CCWGG 3 cut(s) 45, 197, 1180
MvnI CGCG 1 cut(s) 977
MwoI GCNNNNNNNGC 3 cut(s) 210, 1031, 1214
NarI GGCGCC 1 cut(s) 775
NcoI CCATGG 2 cut(s) 1255, 1455
NdeI CATATG 1 cut(s) 1371
NdeII GATC 5 cut(s) 64, 189, 1162, 1494, 1630
NlaIII CATG 9 cut(s) 218, 428, 437, 956, 1147, 1159, 1237, 1259, 1459
NlaIV GGNNCC 4 cut(s) 97, 525, 776, 1453
NmuCI GTSAC 2 cut(s) 420, 668
NsiI ATGCAT 2 cut(s) 439, 1293
NspI RCATGY 1 cut(s) 1147
NspV TTCGAA 1 cut(s) 1637
PaqCI CACCTGC 1 cut(s) 1208
PfeI GAWTC 2 cut(s) 244, 559
PflFI GACNNNGTC 1 cut(s) 1312
PkrI GCNGC 5 cut(s) 61, 206, 901, 1059, 1617
PleI GAGTC 4 cut(s) 245, 974, 1321, 1705
PluTI GGCGCC 1 cut(s) 778
PmaCI CACGTG 1 cut(s) 365
PmlI CACGTG 1 cut(s) 365
PpsI GAGTC 4 cut(s) 245, 974, 1321, 1705
Ppu21I YACGTR 1 cut(s) 365
PpuMI RGGWCCY 2 cut(s) 524, 1356
PshBI ATTAAT 1 cut(s) 12
Psp5II RGGWCCY 2 cut(s) 524, 1356
Psp6I CCWGG 3 cut(s) 43, 195, 1178
PspCI CACGTG 1 cut(s) 365
PspGI CCWGG 3 cut(s) 43, 195, 1178
PspN4I GGNNCC 4 cut(s) 97, 525, 776, 1453
PspPI GGNCC 5 cut(s) 224, 289, 347, 524, 1356
PspPPI RGGWCCY 2 cut(s) 524, 1356
PstI CTGCAG 2 cut(s) 619, 904
PstNI CAGNNNCTG 3 cut(s) 392, 623, 1223
PsuI RGATCY 1 cut(s) 1630
PsyI GACNNNGTC 1 cut(s) 1312
RsaI GTAC 4 cut(s) 172, 340, 549, 909
RsaNI GTAC 4 cut(s) 171, 339, 548, 908
RseI CAYNNNNRTG 2 cut(s) 1460, 1565
SatI GCNGC 5 cut(s) 60, 205, 900, 1058, 1616
Sau3AI GATC 5 cut(s) 64, 189, 1162, 1494, 1630
Sau96I GGNCC 5 cut(s) 224, 289, 347, 524, 1356
SchI GAGTC 4 cut(s) 245, 974, 1322, 1706
ScrFI CCNGG 3 cut(s) 45, 197, 1180
SduI GDGCHC 1 cut(s) 1678
SfaNI GCATC 4 cut(s) 446, 1022, 1216, 1573
SfcI CTRYAG 4 cut(s) 615, 900, 1296, 1404
SfoI GGCGCC 1 cut(s) 776
SfuI TTCGAA 1 cut(s) 1637
SinI GGWCC 4 cut(s) 224, 347, 524, 1356
SmiMI CAYNNNNRTG 2 cut(s) 1460, 1565
SmlI CTYRAG 1 cut(s) 1600
SmoI CTYRAG 1 cut(s) 1600
Sse9I AATT 9 cut(s) 164, 183, 475, 482, 594, 1005, 1062, 1203, 1521
SsiI CCGC 5 cut(s) 60, 977, 1555, 1586, 1671
SspDI GGCGCC 1 cut(s) 774
SspMI CTAG 2 cut(s) 680, 1619
StyD4I CCNGG 3 cut(s) 43, 195, 1178
StyI CCWWGG 4 cut(s) 519, 1045, 1255, 1455
TaaI ACNGT 7 cut(s) 175, 224, 625, 1297, 1415, 1550, 1650
TaiI ACGT 5 cut(s) 367, 405, 412, 660, 789
TaqI TCGA 4 cut(s) 67, 108, 353, 1637
TasI AATT 9 cut(s) 164, 183, 475, 482, 594, 1005, 1062, 1203, 1521
TatI WGTACW 1 cut(s) 170
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 2 cut(s) 244, 559
TscAI CASTG 4 cut(s) 619, 628, 909, 1131
TseFI GTSAC 2 cut(s) 420, 668
TseI GCWGC 4 cut(s) 204, 899, 1057, 1615
Tsp45I GTSAC 2 cut(s) 420, 668
TspDTI ATGAA 3 cut(s) 413, 941, 986
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 4 cut(s) 619, 628, 909, 1131
Tth111I GACNNNGTC 1 cut(s) 1312
VneI GTGCAC 1 cut(s) 1674
VpaK11BI GGWCC 4 cut(s) 224, 347, 524, 1356
VspI ATTAAT 1 cut(s) 12
XceI RCATGY 1 cut(s) 1147
XspI CTAG 2 cut(s) 680, 1619
Zsp2I ATGCAT 2 cut(s) 439, 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.