Rw7G038690

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001739
Physical Location & Seq
Forward (+)
59196963 .. 59198206
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G038690.1

Sequence Viewer

Length: 663 bp
ATGGGGAAGATCGATCATCTTATCTTGGCTACGGAAAATCCTGGTGGGTTCATAAATGGCAAGTGGGAAGGCAACTGGCCCAGTTATCTCTACAAGAGCCTGAGTGAATTTATTGGCTTTGAATATAGACCCAAGAGAGAAAATGGCCAGAAGCAAATGTTCACTGTTAGATCAGTGAGCAGAGCAGTCAAGGCAATCCTAGCTCACCCAATGGTCCTGTGGGGGGGGGTGAACTCGACTCATGAAATTCAGTCTGATGATGATTACCAAGTGGGTCAGGGAAGGGCATTTGCAAGTGGTCGAAGAAAAACTAAGATTGTCTGTACAATTGGTCCTTCCACAAGCTCACGTGAAATGATATGGAAACTTGCAGAAACTGGGATGAATGTAGTGCGTTTGAAAATGTCTCATGGGGACCATACGTCGCACCAGAAAACTATTGATCTTGTCAAAGAATACAACTCTCAATTCAAAGACAAGGTTATAGCCATAATAGTGATAATACTGGACACCAAGGGTCCTGAGGTTAGGAGTGGAGATGTACCACAACCAATTCTGCTTACAGAGGGACAAGAGCTTAACTTTACAATTAGAAGAGGAGTTAGCACCAAAGATACCGTCAGTGTAAACTATGATGACTTTGTGAATGATGTGGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

220

Amino Acids

24.73

Weight (kDa)

8.56

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 102 - 220 8.5e-29 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 145
AcsI RAATTY 2 cut(s) 107, 246
AcvI CACGTG 1 cut(s) 350
AfaI GTAC 2 cut(s) 325, 543
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 3 cut(s) 122, 400, 472
AhdI GACNNNNNGTC 1 cut(s) 421
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 3 cut(s) 203, 345, 577
AluI AGCT 3 cut(s) 203, 345, 577
Alw26I GTCTC 1 cut(s) 411
AlwNI CAGNNNCTG 1 cut(s) 377
AoxI GGCC 2 cut(s) 77, 145
ApoI RAATTY 2 cut(s) 107, 246
ArsI GACNNNNNNTTYG 2 cut(s) 603, 635
AspS9I GGNCC 5 cut(s) 78, 214, 332, 415, 518
AsuHPI GGTGA 2 cut(s) 197, 241
AvaII GGWCC 4 cut(s) 214, 332, 415, 518
AxyI CCTNAGG 1 cut(s) 522
BalI TGGCCA 1 cut(s) 147
BbrPI CACGTG 1 cut(s) 350
BciT130I CCWGG 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 411
BfaI CTAG 1 cut(s) 200
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 4 cut(s) 214, 332, 415, 518
BmeRI GACNNNNNGTC 1 cut(s) 421
BmgT120I GGNCC 5 cut(s) 78, 214, 332, 415, 518
BmiI GGNNCC 2 cut(s) 416, 519
BmrFI CCNGG 1 cut(s) 42
BmrI ACTGGG 2 cut(s) 75, 387
BmuI ACTGGG 2 cut(s) 75, 387
Bsa29I ATCGAT 1 cut(s) 12
BsaAI YACGTR 1 cut(s) 350
BsaJI CCNNGG 1 cut(s) 513
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 4 cut(s) 80, 81, 382, 510
Bse21I CCTNAGG 1 cut(s) 522
BseBI CCWGG 1 cut(s) 42
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 513
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 2 cut(s) 92, 513
BseNI ACTGG 4 cut(s) 80, 81, 382, 510
BseRI GAGGAG 1 cut(s) 612
BshFI GGCC 2 cut(s) 79, 147
BshVI ATCGAT 1 cut(s) 12
BslFI GGGAC 2 cut(s) 428, 582
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 428, 582
BsnI GGCC 2 cut(s) 79, 147
Bsp1407I TGTACA 1 cut(s) 323
Bsp143I GATC 4 cut(s) 9, 13, 170, 442
BspANI GGCC 2 cut(s) 79, 147
BspCNI CTCAG 2 cut(s) 93, 514
BspDI ATCGAT 1 cut(s) 12
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 416, 519
BsrGI TGTACA 1 cut(s) 323
BsrI ACTGG 4 cut(s) 80, 81, 382, 510
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 4 cut(s) 9, 13, 170, 442
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 166, 619
Bst6I CTCTTC 1 cut(s) 589
BstAUI TGTACA 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 350
BstDEI CTNAG 3 cut(s) 101, 312, 522
BstF5I GGATG 1 cut(s) 387
BstKTI GATC 4 cut(s) 12, 16, 173, 445
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 4 cut(s) 9, 13, 170, 442
BstMWI GCNNNNNNNGC 2 cut(s) 191, 200
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 12
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 2 cut(s) 79, 147
BsuTUI ATCGAT 1 cut(s) 12
BtsCI GGATG 1 cut(s) 387
BtsIMutI CAGTG 3 cut(s) 162, 180, 628
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 1 cut(s) 241
Cfr13I GGNCC 5 cut(s) 78, 214, 332, 415, 518
ClaI ATCGAT 1 cut(s) 12
Csp6I GTAC 2 cut(s) 324, 542
CviAII CATG 2 cut(s) 242, 410
CviJI RGCY 9 cut(s) 29, 79, 99, 117, 147, 203, 345, 488, 577
CviKI_1 RGCY 9 cut(s) 29, 79, 99, 117, 147, 203, 345, 488, 577
CviQI GTAC 2 cut(s) 324, 542
DdeI CTNAG 3 cut(s) 101, 312, 522
DpnI GATC 4 cut(s) 11, 15, 172, 444
DpnII GATC 4 cut(s) 9, 13, 170, 442
DriI GACNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 589
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 589
Eco130I CCWWGG 1 cut(s) 513
Eco47I GGWCC 4 cut(s) 214, 332, 415, 518
Eco72I CACGTG 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 2 cut(s) 245, 413
FaiI YATR 9 cut(s) 53, 126, 243, 361, 411, 420, 485, 491, 633
FaqI GGGAC 2 cut(s) 428, 582
FatI CATG 2 cut(s) 241, 409
FokI GGATG 1 cut(s) 394
FspBI CTAG 1 cut(s) 200
HaeIII GGCC 2 cut(s) 79, 147
Hin1II CATG 2 cut(s) 245, 413
HinfI GANTC 1 cut(s) 238
HphI GGTGA 2 cut(s) 197, 241
Hpy166II GTNNAC 3 cut(s) 162, 232, 628
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 2 cut(s) 242, 521
Hpy8I GTNNAC 3 cut(s) 162, 232, 628
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 3 cut(s) 62, 276, 345
HpyCH4III ACNGT 2 cut(s) 166, 619
HpyCH4IV ACGT 2 cut(s) 349, 422
HpyCH4V TGCA 2 cut(s) 293, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 200
HpyF3I CTNAG 3 cut(s) 101, 312, 522
HpySE526I ACGT 2 cut(s) 349, 422
Hsp92II CATG 2 cut(s) 245, 413
Kzo9I GATC 4 cut(s) 9, 13, 170, 442
MaeI CTAG 1 cut(s) 200
MaeII ACGT 2 cut(s) 349, 422
MalI GATC 4 cut(s) 11, 15, 172, 444
MboI GATC 4 cut(s) 9, 13, 170, 442
MboII GAAGA 3 cut(s) 19, 315, 606
MfeI CAATTG 1 cut(s) 327
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 6 cut(s) 107, 246, 327, 467, 552, 588
MluNI TGGCCA 1 cut(s) 147
MlyI GAGTC 1 cut(s) 232
MnlI CCTC 4 cut(s) 517, 559, 590, 649
Mox20I TGGCCA 1 cut(s) 147
MscI TGGCCA 1 cut(s) 147
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 1 cut(s) 494
Msp20I TGGCCA 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 42
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 2 cut(s) 191, 200
NdeII GATC 4 cut(s) 9, 13, 170, 442
NlaIII CATG 2 cut(s) 245, 413
NlaIV GGNNCC 2 cut(s) 416, 519
PagI TCATGA 1 cut(s) 241
PleI GAGTC 1 cut(s) 232
PmaCI CACGTG 1 cut(s) 350
PmlI CACGTG 1 cut(s) 350
PpsI GAGTC 1 cut(s) 232
Ppu21I YACGTR 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 518
Psp5II RGGWCCY 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 40
PspCI CACGTG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 416, 519
PspPI GGNCC 5 cut(s) 78, 214, 332, 415, 518
PspPPI RGGWCCY 1 cut(s) 518
PstNI CAGNNNCTG 1 cut(s) 377
RsaI GTAC 2 cut(s) 325, 543
RsaNI GTAC 2 cut(s) 324, 542
RseI CAYNNNNRTG 1 cut(s) 494
SaqAI TTAA 1 cut(s) 579
Sau3AI GATC 4 cut(s) 9, 13, 170, 442
Sau96I GGNCC 5 cut(s) 78, 214, 332, 415, 518
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 1 cut(s) 42
SetI ASST 8 cut(s) 205, 347, 352, 425, 483, 528, 579, 660
SinI GGWCC 4 cut(s) 214, 332, 415, 518
SmiMI CAYNNNNRTG 1 cut(s) 494
Sse9I AATT 6 cut(s) 107, 246, 327, 467, 552, 588
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 2 cut(s) 166, 619
TaiI ACGT 2 cut(s) 352, 425
TaqI TCGA 3 cut(s) 12, 236, 301
TasI AATT 6 cut(s) 107, 246, 327, 467, 552, 588
TatI WGTACW 1 cut(s) 323
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 3 cut(s) 169, 180, 628
TspDTI ATGAA 3 cut(s) 40, 258, 398
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 3 cut(s) 169, 180, 628
VpaK11BI GGWCC 4 cut(s) 214, 332, 415, 518
XapI RAATTY 2 cut(s) 107, 246
XcmI CCANNNNNNNNNTGG 1 cut(s) 216
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.