RLG00000013234

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001739
Physical Location & Seq
Forward (+)
28132735 .. 28133914
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013234

Sequence Viewer

Length: 576 bp
ATGTTCACTGTTAGATCAGTGAGGATCACCCAGCAGAGCAGTGAAGGCAATCCTAGCTCACCCAATGGTCCTGTGGGTGGTGTTGGGGTGAACTCGACTTATGAAATTCTGTCTGATGATGATTACGAAGTGGGTCAGGGAAGGGCATTTGTAAGTGGTCGAAGAAAAACCAAGATTGTCTGTACAATTGATCCTTCCACAAGCTCACGTGAAATGACATGGAAACTTGCAGAAACTGGGATGAATGTAGTGCGTTTGAATAGGTCTCATGGGGACCATACGTCACACCAGAAAACTATTGATCTTGTCAAAGAATACAACTCTCAATTCAAAGACAAGGTTATAGCCATAATGCTGGACACCAAGGAGCTCAGGGTCTTTAGTATATCAGTCTCTTCTATAGAAAAAGACTGGGAAGATATCAAGTTTCAAGTGGACAATGGGGTTGATTTCTATGCTGTCTCATTTGTAAAAAGTGCTGCGGTGGTTCATGAGTTGAAAGATTATCCCAAAGGTTGTAATGCAGATATTCATGTTATCATAAAAATTGAGAGTGCAGACTATACCAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

192

Amino Acids

21.3

Weight (kDa)

6.21

Isoelectric Point (pI)

32.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 55 - 123 1.2e-19 Pyruvate kinase, barrel domain
PK PF00224 135 - 187 6.7e-08 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 482
AclWI GGATC 2 cut(s) 32, 185
AcsI RAATTY 1 cut(s) 105
AcvI CACGTG 1 cut(s) 209
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 4 cut(s) 259, 331, 431, 499
AhdI GACNNNNNGTC 1 cut(s) 280
AluBI AGCT 3 cut(s) 57, 204, 370
AluI AGCT 3 cut(s) 57, 204, 370
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 3 cut(s) 270, 397, 466
AlwI GGATC 2 cut(s) 32, 185
AlwNI CAGNNNCTG 1 cut(s) 236
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 105
AspS9I GGNCC 2 cut(s) 68, 274
AsuHPI GGTGA 3 cut(s) 19, 51, 100
AvaII GGWCC 2 cut(s) 68, 274
BanII GRGCYC 1 cut(s) 372
BbrPI CACGTG 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 270, 397, 466
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 399
BisI GCNGC 1 cut(s) 480
BlsI GCNGC 1 cut(s) 481
Bme18I GGWCC 2 cut(s) 68, 274
BmeRI GACNNNNNGTC 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 68, 274
BmiI GGNNCC 1 cut(s) 275
BmrI ACTGGG 2 cut(s) 246, 421
BmuI ACTGGG 2 cut(s) 246, 421
Bpu10I CCTNAGC 1 cut(s) 371
BsaAI YACGTR 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 363
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 241, 416
BseDI CCNNGG 1 cut(s) 363
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 1 cut(s) 385
BseNI ACTGG 2 cut(s) 241, 416
BseXI GCAGC 1 cut(s) 466
BseYI CCCAGC 1 cut(s) 30
BsgI GTGCAG 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 372
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 3 cut(s) 270, 397, 466
BsmFI GGGAC 1 cut(s) 287
Bso31I GGTCTC 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 372
Bsp1407I TGTACA 1 cut(s) 182
Bsp143I GATC 4 cut(s) 14, 24, 190, 301
BspACI CCGC 1 cut(s) 482
BspCNI CTCAG 1 cut(s) 384
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 275
BspPI GGATC 2 cut(s) 32, 185
BspTNI GGTCTC 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 182
BsrI ACTGG 2 cut(s) 241, 416
BssECI CCNNGG 1 cut(s) 363
BssMI GATC 4 cut(s) 14, 24, 190, 301
BssT1I CCWWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 400
BstAUI TGTACA 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 246
BstKTI GATC 4 cut(s) 17, 27, 193, 304
BstMAI GTCTC 3 cut(s) 270, 397, 466
BstMBI GATC 4 cut(s) 14, 24, 190, 301
BstMWI GCNNNNNNNGC 2 cut(s) 45, 54
BstSFI CTRYAG 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 355
BtsCI GGATG 1 cut(s) 246
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 3 cut(s) 6, 24, 46
CaiI CAGNNNCTG 1 cut(s) 236
CciI TCATGA 1 cut(s) 490
Cfr13I GGNCC 2 cut(s) 68, 274
Csp6I GTAC 1 cut(s) 183
CviAII CATG 4 cut(s) 219, 269, 491, 533
CviJI RGCY 4 cut(s) 57, 204, 347, 370
CviKI_1 RGCY 4 cut(s) 57, 204, 347, 370
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 1 cut(s) 371
DpnI GATC 4 cut(s) 16, 26, 192, 303
DpnII GATC 4 cut(s) 14, 24, 190, 301
DriI GACNNNNNGTC 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 400
Eam1105I GACNNNNNGTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 400
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 270
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 2 cut(s) 68, 274
Eco53kI GAGCTC 1 cut(s) 370
Eco72I CACGTG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 370
EcoRV GATATC 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 4 cut(s) 222, 272, 494, 536
FaqI GGGAC 1 cut(s) 287
FatI CATG 4 cut(s) 218, 268, 490, 532
Fnu4HI GCNGC 1 cut(s) 480
FokI GGATG 1 cut(s) 253
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 1 cut(s) 480
FspBI CTAG 1 cut(s) 54
GluI GCNGC 1 cut(s) 480
GsaI CCCAGC 1 cut(s) 34
Hin1II CATG 4 cut(s) 222, 272, 494, 536
HphI GGTGA 3 cut(s) 19, 51, 100
Hpy166II GTNNAC 3 cut(s) 6, 91, 436
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 2 cut(s) 491, 573
Hpy8I GTNNAC 3 cut(s) 6, 91, 436
HpyAV CCTTC 3 cut(s) 38, 135, 204
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 2 cut(s) 208, 281
HpyCH4V TGCA 3 cut(s) 230, 524, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 54
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 2 cut(s) 208, 281
Hsp92II CATG 4 cut(s) 222, 272, 494, 536
Kzo9I GATC 4 cut(s) 14, 24, 190, 301
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 8 cut(s) 44, 84, 122, 222, 302, 341, 358, 397
Lsp1109I GCAGC 1 cut(s) 466
MaeI CTAG 1 cut(s) 54
MaeII ACGT 2 cut(s) 208, 281
MaeIII GTNAC 1 cut(s) 282
MalI GATC 4 cut(s) 16, 26, 192, 303
MboI GATC 4 cut(s) 14, 24, 190, 301
MboII GAAGA 3 cut(s) 174, 387, 428
MfeI CAATTG 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 372
MluCI AATT 4 cut(s) 105, 186, 326, 546
MnlI CCTC 1 cut(s) 15
MunI CAATTG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 45, 54
NdeII GATC 4 cut(s) 14, 24, 190, 301
NlaIII CATG 4 cut(s) 222, 272, 494, 536
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 282
PagI TCATGA 1 cut(s) 490
PkrI GCNGC 1 cut(s) 481
PmaCI CACGTG 1 cut(s) 209
PmlI CACGTG 1 cut(s) 209
Ppu21I YACGTR 1 cut(s) 209
Psp124BI GAGCTC 1 cut(s) 372
PspCI CACGTG 1 cut(s) 209
PspFI CCCAGC 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 2 cut(s) 68, 274
PstNI CAGNNNCTG 1 cut(s) 236
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
SacI GAGCTC 1 cut(s) 372
SatI GCNGC 1 cut(s) 480
Sau3AI GATC 4 cut(s) 14, 24, 190, 301
Sau96I GGNCC 2 cut(s) 68, 274
SduI GDGCHC 1 cut(s) 372
SetI ASST 8 cut(s) 59, 206, 211, 266, 284, 342, 372, 517
SfcI CTRYAG 1 cut(s) 399
SinI GGWCC 2 cut(s) 68, 274
Sse9I AATT 4 cut(s) 105, 186, 326, 546
SsiI CCGC 1 cut(s) 482
SspMI CTAG 1 cut(s) 54
SstI GAGCTC 1 cut(s) 372
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 2 cut(s) 211, 284
TaqI TCGA 2 cut(s) 95, 160
TasI AATT 4 cut(s) 105, 186, 326, 546
TatI WGTACW 1 cut(s) 182
TscAI CASTG 3 cut(s) 13, 24, 46
TseFI GTSAC 1 cut(s) 282
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 282
TspDTI ATGAA 4 cut(s) 117, 257, 479, 521
TspRI CASTG 3 cut(s) 13, 24, 46
VpaK11BI GGWCC 2 cut(s) 68, 274
XapI RAATTY 1 cut(s) 105
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.