RLG00000001114

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001739
Physical Location & Seq
Reverse (-)
10478994 .. 10483846
4853 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001114

Sequence Viewer

Length: 933 bp
ATGTTCACTGTTAGATCAGTGAGGATCACGCAGCAGAGCAGTGAATATGGGGGGGTGAACTCAACTTATGAAATTCAGTCTGATGATGATTACAAAGTGGGTCAGGGAAGGGCATTTGCAAGTGGTCGAAGAAAAACTAAGATTGTCTGTACAATTGGTCCTTCCACAAGCTCACGTGAAATGATATGGAAACTTGCAGAAACTGGGATGAATGTAGTGCGTTTGAAAATGTCTCATGGGGACCATACGTTGCACCTGAAAACTATTGATCTTGTCAAAGAATACAACTCTCAATTCAAAGACAAGGTTATAGCCATAATACTGGACACCAAGGGTCCTGAGGTTAGGAGTGGAGATGTACCACAACCAATTCTGCTTACAGAGGGACAAGAGCTTAACTTTACAATTAGAAGAGGATTCTGGTTGATGATTTTGCTTTCAGCTTTCGAGTGTGCGCAGCGCAATGAGAGGAACAATGAGTTCAGTTTGCTGATGCTTAATAGACAAGTGGTGAAATCTGAGTTTCCTACTATTAAGGTGCTTGGAATGGATCTTGAATTAGTGATGGCAATTCTTTATACATATTTGCTTTTCCATCTTGCGTTTTGCCAAGTGACAGTTTTCTTACAAGGTGAGGCTGCAGTTTGTTCTTGGCTGAAGAGAACTAAGAAGGTTCCGAAGAGGAGTGCAAAATCAAATCAGAAACTGGCAAAGAAATATGAGAATAACAAAGACGACGAAGAGGATCAAGAAGAAGCTGATGAAGATGAGAAGGAAGAGAATGATGCCCAGGACTCATATGTTTGTGACGAGAAGATTGGGTTGAAAACAAGTGGAACAGATGGCAATAGTTTCTCGGCCCTGGGTTGTTGGGTTGTTGGAATCTTCATTTCTGTTCCTCCAACTCACTCAGAAGACTTGCATCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

311

Amino Acids

35.1

Weight (kDa)

5.79

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 44 - 137 9.3e-26 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 456
AclWI GGATC 3 cut(s) 32, 558, 753
AcsI RAATTY 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 677
AcvI CACGTG 1 cut(s) 176
AfaI GTAC 2 cut(s) 151, 360
AgsI TTSAA 4 cut(s) 226, 298, 557, 826
AjnI CCWGG 2 cut(s) 789, 861
AluBI AGCT 4 cut(s) 171, 394, 443, 758
AluI AGCT 4 cut(s) 171, 394, 443, 758
Alw26I GTCTC 1 cut(s) 237
AlwI GGATC 3 cut(s) 32, 558, 753
AlwNI CAGNNNCTG 2 cut(s) 203, 706
AoxI GGCC 1 cut(s) 858
ApeKI GCWGC 3 cut(s) 31, 457, 638
ApoI RAATTY 1 cut(s) 72
AspLEI GCGC 2 cut(s) 457, 462
AspS9I GGNCC 4 cut(s) 158, 241, 335, 859
AsuHPI GGTGA 3 cut(s) 67, 523, 644
AvaII GGWCC 3 cut(s) 158, 241, 335
AxyI CCTNAGG 1 cut(s) 339
BbrPI CACGTG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 921
BbvI GCAGC 3 cut(s) 43, 469, 625
BccI CCATC 3 cut(s) 559, 603, 836
BciT130I CCWGG 2 cut(s) 791, 863
BcoDI GTCTC 1 cut(s) 237
BfmI CTRYAG 1 cut(s) 639
BisI GCNGC 3 cut(s) 32, 458, 639
BlsI GCNGC 3 cut(s) 33, 459, 640
Bme1390I CCNGG 2 cut(s) 791, 863
Bme18I GGWCC 3 cut(s) 158, 241, 335
BmgT120I GGNCC 4 cut(s) 158, 241, 335, 859
BmiI GGNNCC 3 cut(s) 242, 336, 675
BmrFI CCNGG 2 cut(s) 791, 863
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 2 cut(s) 483, 775
BmuI ACTGGG 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 921
BsaAI YACGTR 1 cut(s) 176
BsaJI CCNNGG 4 cut(s) 330, 789, 861, 862
Bse1I ACTGG 3 cut(s) 208, 327, 711
Bse21I CCTNAGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 469
BseBI CCWGG 2 cut(s) 791, 863
BseDI CCNNGG 4 cut(s) 330, 789, 861, 862
BseGI GGATG 2 cut(s) 213, 922
BseMI GCAATG 1 cut(s) 469
BseMII CTCAG 3 cut(s) 330, 510, 924
BseNI ACTGG 3 cut(s) 208, 327, 711
BseRI GAGGAG 1 cut(s) 697
BseXI GCAGC 3 cut(s) 43, 469, 625
BshFI GGCC 1 cut(s) 860
BslFI GGGAC 2 cut(s) 254, 399
BsmAI GTCTC 1 cut(s) 237
BsmFI GGGAC 2 cut(s) 254, 399
BsnI GGCC 1 cut(s) 860
Bsp1407I TGTACA 1 cut(s) 149
Bsp143I GATC 5 cut(s) 14, 24, 268, 550, 745
BspANI GGCC 1 cut(s) 860
BspCNI CTCAG 3 cut(s) 331, 511, 923
BspLI GGNNCC 3 cut(s) 242, 336, 675
BspMAI CTGCAG 1 cut(s) 643
BspPI GGATC 3 cut(s) 32, 558, 753
BsrDI GCAATG 1 cut(s) 469
BsrGI TGTACA 1 cut(s) 149
BsrI ACTGG 3 cut(s) 208, 327, 711
BssECI CCNNGG 4 cut(s) 330, 789, 861, 862
BssMI GATC 5 cut(s) 14, 24, 268, 550, 745
BssT1I CCWWGG 1 cut(s) 330
Bst2UI CCWGG 2 cut(s) 791, 863
Bst4CI ACNGT 2 cut(s) 10, 619
Bst6I CTCTTC 5 cut(s) 406, 653, 674, 735, 771
BstAUI TGTACA 1 cut(s) 149
BstBAI YACGTR 1 cut(s) 176
BstDEI CTNAG 5 cut(s) 138, 339, 519, 666, 910
BstF5I GGATG 2 cut(s) 213, 922
BstHHI GCGC 2 cut(s) 457, 462
BstKTI GATC 5 cut(s) 17, 27, 271, 553, 748
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 5 cut(s) 14, 24, 268, 550, 745
BstNI CCWGG 2 cut(s) 791, 863
BstSCI CCNGG 2 cut(s) 789, 861
BstSFI CTRYAG 1 cut(s) 639
BstV1I GCAGC 3 cut(s) 43, 469, 625
BstV2I GAAGAC 1 cut(s) 921
BstX2I RGATCY 1 cut(s) 550
BstXI CCANNNNNNTGG 1 cut(s) 322
BstYI RGATCY 1 cut(s) 550
Bsu36I CCTNAGG 1 cut(s) 339
BsuRI GGCC 1 cut(s) 860
BtsCI GGATG 2 cut(s) 213, 922
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 3 cut(s) 6, 24, 46
CaiI CAGNNNCTG 2 cut(s) 203, 706
CfoI GCGC 2 cut(s) 457, 462
Cfr13I GGNCC 4 cut(s) 158, 241, 335, 859
Csp6I GTAC 2 cut(s) 150, 359
CviAII CATG 1 cut(s) 236
CviJI RGCY 8 cut(s) 171, 314, 394, 443, 638, 655, 758, 860
CviKI_1 RGCY 8 cut(s) 171, 314, 394, 443, 638, 655, 758, 860
CviQI GTAC 2 cut(s) 150, 359
DdeI CTNAG 5 cut(s) 138, 339, 519, 666, 910
DpnI GATC 5 cut(s) 16, 26, 270, 552, 747
DpnII GATC 5 cut(s) 14, 24, 268, 550, 745
Eam1104I CTCTTC 5 cut(s) 406, 653, 674, 735, 771
EarI CTCTTC 5 cut(s) 406, 653, 674, 735, 771
Eco130I CCWWGG 1 cut(s) 330
Eco47I GGWCC 3 cut(s) 158, 241, 335
Eco57I CTGAAG 1 cut(s) 677
Eco72I CACGTG 1 cut(s) 176
Eco81I CCTNAGG 1 cut(s) 339
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 789, 861
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 1 cut(s) 239
FaqI GGGAC 2 cut(s) 254, 399
FatI CATG 1 cut(s) 235
FauNDI CATATG 1 cut(s) 799
Fnu4HI GCNGC 3 cut(s) 32, 458, 639
FokI GGATG 2 cut(s) 220, 909
Fsp4HI GCNGC 3 cut(s) 32, 458, 639
FspI TGCGCA 1 cut(s) 456
GlaI GCGC 2 cut(s) 456, 461
GluI GCNGC 3 cut(s) 32, 458, 639
HaeIII GGCC 1 cut(s) 860
HhaI GCGC 2 cut(s) 457, 462
Hin1II CATG 1 cut(s) 239
Hin6I GCGC 2 cut(s) 455, 460
HinP1I GCGC 2 cut(s) 455, 460
HinfI GANTC 3 cut(s) 417, 794, 882
HphI GGTGA 3 cut(s) 67, 523, 644
Hpy166II GTNNAC 2 cut(s) 6, 58
Hpy188I TCNGA 5 cut(s) 82, 520, 678, 702, 913
Hpy188III TCNNGA 3 cut(s) 338, 554, 749
Hpy8I GTNNAC 2 cut(s) 6, 58
Hpy99I CGWCG 1 cut(s) 740
HpyAV CCTTC 4 cut(s) 102, 171, 664, 766
HpyCH4III ACNGT 2 cut(s) 10, 619
HpyCH4IV ACGT 2 cut(s) 175, 248
HpyCH4V TGCA 6 cut(s) 119, 197, 253, 641, 689, 922
HpyF3I CTNAG 5 cut(s) 138, 339, 519, 666, 910
HpySE526I ACGT 2 cut(s) 175, 248
Hsp92II CATG 1 cut(s) 239
HspAI GCGC 2 cut(s) 455, 460
Kzo9I GATC 5 cut(s) 14, 24, 268, 550, 745
Lsp1109I GCAGC 3 cut(s) 43, 469, 625
LweI GCATC 2 cut(s) 483, 775
MaeII ACGT 2 cut(s) 175, 248
MaeIII GTNAC 2 cut(s) 613, 806
MalI GATC 5 cut(s) 16, 26, 270, 552, 747
MboI GATC 5 cut(s) 14, 24, 268, 550, 745
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 1 cut(s) 550
MluCI AATT 7 cut(s) 72, 153, 293, 369, 405, 557, 570
MlyI GAGTC 1 cut(s) 788
MmeI TCCRAC 2 cut(s) 859, 926
MnlI CCTC 9 cut(s) 15, 334, 376, 407, 462, 628, 675, 736, 909
MseI TTAA 3 cut(s) 396, 498, 534
MspR9I CCNGG 2 cut(s) 791, 863
MteI GCGCNGCGC 1 cut(s) 458
MunI CAATTG 1 cut(s) 153
MvaI CCWGG 2 cut(s) 791, 863
NdeI CATATG 1 cut(s) 799
NdeII GATC 5 cut(s) 14, 24, 268, 550, 745
NlaIII CATG 1 cut(s) 239
NlaIV GGNNCC 3 cut(s) 242, 336, 675
NmeAIII GCCGAG 1 cut(s) 836
NmuCI GTSAC 2 cut(s) 613, 806
NsbI TGCGCA 1 cut(s) 456
PasI CCCWGGG 1 cut(s) 862
PfeI GAWTC 2 cut(s) 417, 882
PkrI GCNGC 3 cut(s) 33, 459, 640
PleI GAGTC 1 cut(s) 788
PmaCI CACGTG 1 cut(s) 176
PmlI CACGTG 1 cut(s) 176
PpsI GAGTC 1 cut(s) 788
Ppu21I YACGTR 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 789, 861
PspCI CACGTG 1 cut(s) 176
PspGI CCWGG 2 cut(s) 789, 861
PspN4I GGNNCC 3 cut(s) 242, 336, 675
PspPI GGNCC 4 cut(s) 158, 241, 335, 859
PspPPI RGGWCCY 1 cut(s) 335
PstI CTGCAG 1 cut(s) 643
PstNI CAGNNNCTG 2 cut(s) 203, 706
PsuI RGATCY 1 cut(s) 550
RsaI GTAC 2 cut(s) 151, 360
RsaNI GTAC 2 cut(s) 150, 359
SaqAI TTAA 3 cut(s) 396, 498, 534
SatI GCNGC 3 cut(s) 32, 458, 639
Sau3AI GATC 5 cut(s) 14, 24, 268, 550, 745
Sau96I GGNCC 4 cut(s) 158, 241, 335, 859
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 2 cut(s) 791, 863
SfaNI GCATC 2 cut(s) 483, 775
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 3 cut(s) 158, 241, 335
Sse9I AATT 7 cut(s) 72, 153, 293, 369, 405, 557, 570
StyD4I CCNGG 2 cut(s) 789, 861
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 2 cut(s) 10, 619
TaiI ACGT 2 cut(s) 178, 251
TaqI TCGA 2 cut(s) 127, 447
TasI AATT 7 cut(s) 72, 153, 293, 369, 405, 557, 570
TatI WGTACW 1 cut(s) 149
TfiI GAWTC 2 cut(s) 417, 882
Tru1I TTAA 3 cut(s) 396, 498, 534
Tru9I TTAA 3 cut(s) 396, 498, 534
TscAI CASTG 3 cut(s) 13, 24, 46
TseFI GTSAC 2 cut(s) 613, 806
TseI GCWGC 3 cut(s) 31, 457, 638
Tsp45I GTSAC 2 cut(s) 613, 806
TspDTI ATGAA 4 cut(s) 84, 224, 777, 877
TspRI CASTG 3 cut(s) 13, 24, 46
VpaK11BI GGWCC 3 cut(s) 158, 241, 335
XapI RAATTY 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.