Rw7G020500

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001739
Physical Location & Seq
Forward (+)
23324907 .. 23332804
7898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020500.1

Sequence Viewer

Length: 1722 bp
ATGGCGACGCTTAATGTTCCGGCCAGAATGTCCCTGAAACATGATCGGACTTGGACAACTGATCGGTTGGCTTCCTCCAAGAGCCTGAGTGAATTTATTGGCTTTGAATATAGACCCAAGAGAGAAAATGGCCAGAAGCAAATGTTCACTGTTAGATCAGTGAGGATCACCCAGCAGAGCAGTGAAGGCAATCCTAGCTCAACCAATGGCCCTGTGGGTGGTGTTGGGGTGAACTCGACTTATGAAATTCAGTCTGATGATGATTACGAAGTGGGTCAGGGAAGGGCATTTGCAAGTGGTCGAAGAAAAACTAAGATTGTCTGTACAATTGGTCCTTCCACGAGCTCACGTGAAATGATATGGAAACTTGCAGAAACTGGGATGAATGTAGCGCGTTTGAATATGTCTCATGGGGACCATACGTCGCACCAGAAAACTATTGATTTTGTCAAAGAATACAACTCTCAATTCAAAGACAAGGTTATAGCCATAATGCTGGACACCAAGGGTCCTGAGGTTAGGAGTGGAGATGTACCACAACCAATTCTGCTTACAGAGGGACAAGAGTTTAACTTTACAATTAGAAGGGGAGTTAGCACAAAAGATACCGTTAGTGTAAACTATGATGACTTTGTGAATGATGTGGAGGTTGGAGACATAGTTCTGGTTGATGGTGGAATGATGTCTTTAGCCGTGAAGTCAAAGACAAAAGATTTGGTTAAATGTGTAGTAGTGGATGGAGGAGAACTAAAATCAAGGCGCCATTTAAATGTGCGCGGAAAAAGTGCAACTCTGCCTTCAATAACAGAAGATATCAAGTTTGGAGTGGACAATGGAGTTGATTTCTATGCTGTCTCATTTGTAAAAAGTGCTGCGGTGGTTCATGAGTTGAAAGATTATCTCAAAGGTTGTAATGCAGATATTCATGTTATCGTAAAAATTGAAAGTGCAGACTCTATACCAAATCTTCATTCAATAATTTCCGCATCTGATGGGGCTATGATTGCTCGTGGAGACCTAGGAGCTGAGCTCCCAATTGAGGAAGTTCCTTTGTTGCAGGAAGACATCATTAGAAGGTGTCACAGTATGCAGAAACCAGTTATTGTAGCAACTAATATGTTGGAGAGCATGATTGATCATCCTACACCAACCAGGGCAGAAGTCTCTGACATTGCAATTGCAGTGAGAGAAGGTGCTGATGCAATCATGCTATCTGGAGAAACTGCCCATGGAAAATATCCACTCAAAGCTGTTAAAGTAATGCATACTGTGGCATTGAGGACTGAGTCAAGTCTACCAATTAGCACTATTCCTCCGATCCAAATAAGTACCTATAAGAGACATATGGGGGAAATGTTTGCTTTCCACTCTACCACTATGGCCAACAGTCTCAATACACCCATCATTGTTTTCACAAGGACAGGATCCATGGCTGTACTTTTAAGCCATTATAGGCCATGCTCAACAATCTTTGCCTTCACAAATGAAGAAAGGATTAAGCAGAGATTGGTGCTTTATCATGGTGTCCTCCCAATATATATGCAATTTTCAAATGATTCAGAGGAGACTTTCTCAAGAGCAATAAAGCTATTAGTGGTTAAGGGTCATTTGAAAGAGGGAGAGCATGTTACTCTTGTCCAAAGTGGTGCACAACCAATCTGGCGTCAAGAATCTTCTCATCATATCCAAGTTCGTAAAGTGGAAAGTATTATGAACCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

573

Amino Acids

63.39

Weight (kDa)

7.34

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 102 - 418 3.3e-106 Pyruvate kinase, barrel domain
PK_C PF02887 459 - 547 5.5e-17 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 759
AccI GTMKAC 1 cut(s) 1294
AccII CGCG 2 cut(s) 394, 777
AciI CCGC 3 cut(s) 777, 875, 984
AclWI GGATC 4 cut(s) 173, 1312, 1419, 1432
AcoI YGGCCR 3 cut(s) 21, 130, 1380
AcsI RAATTY 2 cut(s) 92, 246
AcvI CACGTG 1 cut(s) 350
AcyI GRCGYC 2 cut(s) 760, 1663
AfaI GTAC 4 cut(s) 325, 534, 1330, 1437
AfiI CCNNNNNNNGG 2 cut(s) 218, 1039
AgsI TTSAA 9 cut(s) 107, 400, 472, 801, 892, 944, 975, 1551, 1612
AhdI GACNNNNNGTC 1 cut(s) 421
AjnI CCWGG 1 cut(s) 1151
AjuI GAANNNNNNNTTGG 2 cut(s) 955, 987
AloI GAACNNNNNNTCC 2 cut(s) 645, 677
AluBI AGCT 6 cut(s) 198, 345, 1025, 1030, 1250, 1588
AluI AGCT 6 cut(s) 198, 345, 1025, 1030, 1250, 1588
Alw21I GWGCWC 3 cut(s) 347, 1032, 1651
Alw26I GTCTC 8 cut(s) 411, 648, 859, 1008, 1168, 1333, 1394, 1559
Alw44I GTGCAC 1 cut(s) 1647
AlwI GGATC 4 cut(s) 173, 1312, 1419, 1432
AlwNI CAGNNNCTG 1 cut(s) 377
AoxI GGCC 5 cut(s) 21, 130, 208, 1380, 1454
ApaLI GTGCAC 1 cut(s) 1647
ApeKI GCWGC 1 cut(s) 872
ApoI RAATTY 2 cut(s) 92, 246
ArsI GACNNNNNNTTYG 2 cut(s) 697, 729
AspA2I CCTAGG 1 cut(s) 1018
AspLEI GCGC 3 cut(s) 394, 762, 777
AspS9I GGNCC 4 cut(s) 209, 332, 415, 509
AsuHPI GGTGA 2 cut(s) 160, 241
AvaII GGWCC 3 cut(s) 332, 415, 509
AvrII CCTAGG 1 cut(s) 1018
AxyI CCTNAGG 1 cut(s) 513
BaeGI GKGCMC 1 cut(s) 1651
BalI TGGCCA 2 cut(s) 132, 1382
BamHI GGATCC 1 cut(s) 1424
BanI GGYRCC 1 cut(s) 759
BanII GRGCYC 2 cut(s) 347, 1032
BauI CACGAG 2 cut(s) 340, 1008
BbrPI CACGTG 1 cut(s) 350
BbsI GAAGAC 1 cut(s) 1068
Bbv12I GWGCWC 3 cut(s) 347, 1032, 1651
BbvI GCAGC 1 cut(s) 859
BccI CCATC 4 cut(s) 665, 731, 986, 1409
BceAI ACGGC 1 cut(s) 677
BciT130I CCWGG 1 cut(s) 1153
BclI TGATCA 1 cut(s) 1135
BcoDI GTCTC 8 cut(s) 411, 648, 859, 1008, 1168, 1333, 1394, 1559
BfaI CTAG 2 cut(s) 195, 1019
BfoI RGCGCY 1 cut(s) 763
BisI GCNGC 1 cut(s) 873
BlnI CCTAGG 1 cut(s) 1018
BlpI GCTNAGC 1 cut(s) 1026
BlsI GCNGC 1 cut(s) 874
Bme1390I CCNGG 1 cut(s) 1153
Bme18I GGWCC 3 cut(s) 332, 415, 509
BmeRI GACNNNNNGTC 1 cut(s) 421
BmgT120I GGNCC 4 cut(s) 209, 332, 415, 509
BmiI GGNNCC 4 cut(s) 416, 510, 761, 1426
BmrFI CCNGG 1 cut(s) 1153
BmrI ACTGGG 1 cut(s) 387
BmsI GCATC 2 cut(s) 995, 1189
BmuI ACTGGG 1 cut(s) 387
BpiI GAAGAC 1 cut(s) 1068
BplI GAGNNNNNCTC 4 cut(s) 1014, 1046, 1556, 1588
BpmI CTGGAG 1 cut(s) 1236
Bpu1102I GCTNAGC 1 cut(s) 1026
BpuEI CTTGAG 1 cut(s) 1558
BsaAI YACGTR 1 cut(s) 350
BsaHI GRCGYC 2 cut(s) 760, 1663
BsaI GGTCTC 1 cut(s) 1008
BsaJI CCNNGG 5 cut(s) 504, 1018, 1152, 1228, 1428
BsaXI ACNNNNNCTCC 6 cut(s) 645, 675, 1297, 1327, 1611, 1641
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 1039
Bse1I ACTGG 2 cut(s) 382, 1097
Bse21I CCTNAGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 1170
BseBI CCWGG 1 cut(s) 1153
BseDI CCNNGG 5 cut(s) 504, 1018, 1152, 1228, 1428
BseGI GGATG 3 cut(s) 387, 742, 1138
BseLI CCNNNNNNNGG 2 cut(s) 218, 1039
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 4 cut(s) 77, 504, 1017, 1275
BseNI ACTGG 2 cut(s) 382, 1097
BseRI GAGGAG 2 cut(s) 756, 1577
BseSI GKGCMC 1 cut(s) 1651
BseXI GCAGC 1 cut(s) 859
BseYI CCCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 969
Bsh1236I CGCG 2 cut(s) 394, 777
BshFI GGCC 5 cut(s) 23, 132, 210, 1382, 1456
BshNI GGYRCC 1 cut(s) 759
BsiHKAI GWGCWC 3 cut(s) 347, 1032, 1651
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 3 cut(s) 16, 428, 573
BslI CCNNNNNNNGG 2 cut(s) 218, 1039
BsmAI GTCTC 8 cut(s) 411, 648, 859, 1008, 1168, 1333, 1394, 1559
BsmFI GGGAC 3 cut(s) 16, 428, 573
BsnI GGCC 5 cut(s) 23, 132, 210, 1382, 1456
Bso31I GGTCTC 1 cut(s) 1008
Bsp1286I GDGCHC 3 cut(s) 347, 1032, 1651
Bsp1407I TGTACA 1 cut(s) 323
Bsp143I GATC 7 cut(s) 43, 61, 155, 165, 1135, 1317, 1424
Bsp1720I GCTNAGC 1 cut(s) 1026
Bsp19I CCATGG 2 cut(s) 1228, 1428
BspACI CCGC 3 cut(s) 777, 875, 984
BspANI GGCC 5 cut(s) 23, 132, 210, 1382, 1456
BspCNI CTCAG 4 cut(s) 78, 505, 1018, 1276
BspFNI CGCG 2 cut(s) 394, 777
BspHI TCATGA 1 cut(s) 883
BspLI GGNNCC 4 cut(s) 416, 510, 761, 1426
BspPI GGATC 4 cut(s) 173, 1312, 1419, 1432
BspT107I GGYRCC 1 cut(s) 759
BspTNI GGTCTC 1 cut(s) 1008
BsrDI GCAATG 1 cut(s) 1170
BsrGI TGTACA 1 cut(s) 323
BsrI ACTGG 2 cut(s) 382, 1097
BssECI CCNNGG 5 cut(s) 504, 1018, 1152, 1228, 1428
BssMI GATC 7 cut(s) 43, 61, 155, 165, 1135, 1317, 1424
BssNI GRCGYC 2 cut(s) 760, 1663
BssSI CACGAG 2 cut(s) 340, 1008
BssT1I CCWWGG 4 cut(s) 504, 1018, 1228, 1428
Bst2BI CACGAG 2 cut(s) 340, 1008
Bst2UI CCWGG 1 cut(s) 1153
Bst4CI ACNGT 5 cut(s) 151, 610, 1085, 1270, 1388
BstACI GRCGYC 2 cut(s) 760, 1663
BstAUI TGTACA 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 350
BstDEI CTNAG 5 cut(s) 86, 312, 513, 1026, 1284
BstDSI CCRYGG 2 cut(s) 1228, 1428
BstF5I GGATG 3 cut(s) 387, 742, 1138
BstFNI CGCG 2 cut(s) 394, 777
BstH2I RGCGCY 1 cut(s) 763
BstHHI GCGC 3 cut(s) 394, 762, 777
BstKTI GATC 7 cut(s) 46, 64, 158, 168, 1138, 1320, 1427
BstMAI GTCTC 8 cut(s) 411, 648, 859, 1008, 1168, 1333, 1394, 1559
BstMBI GATC 7 cut(s) 43, 61, 155, 165, 1135, 1317, 1424
BstMWI GCNNNNNNNGC 3 cut(s) 186, 195, 1004
BstNI CCWGG 1 cut(s) 1153
BstNSI RCATGY 1 cut(s) 1628
BstSCI CCNGG 1 cut(s) 1151
BstSLI GKGCMC 1 cut(s) 1651
BstUI CGCG 2 cut(s) 394, 777
BstV1I GCAGC 1 cut(s) 859
BstV2I GAAGAC 1 cut(s) 1068
BstX2I RGATCY 1 cut(s) 1424
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 1 cut(s) 1424
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 5 cut(s) 23, 132, 210, 1382, 1456
BtgI CCRYGG 2 cut(s) 1228, 1428
BtsCI GGATG 3 cut(s) 387, 742, 1138
BtsI GCAGTG 2 cut(s) 187, 1188
BtsIMutI CAGTG 4 cut(s) 147, 165, 187, 1188
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 1 cut(s) 883
CfoI GCGC 3 cut(s) 394, 762, 777
Cfr13I GGNCC 4 cut(s) 209, 332, 415, 509
CseI GACGC 2 cut(s) 16, 1652
Csp6I GTAC 4 cut(s) 324, 533, 1329, 1436
CviQI GTAC 4 cut(s) 324, 533, 1329, 1436
DdeI CTNAG 5 cut(s) 86, 312, 513, 1026, 1284
DinI GGCGCC 1 cut(s) 761
DpnI GATC 7 cut(s) 45, 63, 157, 167, 1137, 1319, 1426
DpnII GATC 7 cut(s) 43, 61, 155, 165, 1135, 1317, 1424
DraI TTTAAA 1 cut(s) 768
DriI GACNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 3 cut(s) 21, 130, 1380
Eam1105I GACNNNNNGTC 1 cut(s) 421
Ecl136II GAGCTC 2 cut(s) 345, 1030
Eco130I CCWWGG 4 cut(s) 504, 1018, 1228, 1428
Eco24I GRGCYC 2 cut(s) 347, 1032
Eco31I GGTCTC 1 cut(s) 1008
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 3 cut(s) 332, 415, 509
Eco53kI GAGCTC 2 cut(s) 345, 1030
Eco72I CACGTG 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 513
EcoICRI GAGCTC 2 cut(s) 345, 1030
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 1151
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 4 cut(s) 504, 1018, 1228, 1428
EcoT22I ATGCAT 1 cut(s) 1266
EcoT38I GRGCYC 2 cut(s) 347, 1032
EgeI GGCGCC 1 cut(s) 761
EheI GGCGCC 1 cut(s) 761
ErhI CCWWGG 4 cut(s) 504, 1018, 1228, 1428
FaqI GGGAC 3 cut(s) 16, 428, 573
FauNDI CATATG 1 cut(s) 1344
FbaI TGATCA 1 cut(s) 1135
FblI GTMKAC 1 cut(s) 1294
Fnu4HI GCNGC 1 cut(s) 873
FokI GGATG 3 cut(s) 394, 749, 1125
FriOI GRGCYC 2 cut(s) 347, 1032
Fsp4HI GCNGC 1 cut(s) 873
FspBI CTAG 2 cut(s) 195, 1019
GlaI GCGC 3 cut(s) 393, 761, 776
GluI GCNGC 1 cut(s) 873
GsaI CCCAGC 1 cut(s) 175
GsuI CTGGAG 1 cut(s) 1236
HaeII RGCGCY 1 cut(s) 763
HaeIII GGCC 5 cut(s) 23, 132, 210, 1382, 1456
HapII CCGG 1 cut(s) 20
HgaI GACGC 2 cut(s) 16, 1652
HhaI GCGC 3 cut(s) 394, 762, 777
Hin1I GRCGYC 2 cut(s) 760, 1663
Hin6I GCGC 3 cut(s) 392, 760, 775
HinP1I GCGC 3 cut(s) 392, 760, 775
HinfI GANTC 4 cut(s) 953, 1286, 1556, 1670
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 160, 241
Hpy166II GTNNAC 6 cut(s) 147, 232, 619, 829, 1295, 1649
Hpy188I TCNGA 6 cut(s) 48, 256, 991, 1168, 1317, 1561
Hpy188III TCNNGA 5 cut(s) 512, 884, 1215, 1575, 1667
Hpy8I GTNNAC 6 cut(s) 147, 232, 619, 829, 1295, 1649
Hpy99I CGWCG 2 cut(s) 10, 427
HpyAV CCTTC 8 cut(s) 179, 276, 345, 579, 807, 1068, 1184, 1486
HpyCH4III ACNGT 5 cut(s) 151, 610, 1085, 1270, 1388
HpyCH4IV ACGT 2 cut(s) 349, 422
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 195, 1004
HpyF3I CTNAG 5 cut(s) 86, 312, 513, 1026, 1284
HpySE526I ACGT 2 cut(s) 349, 422
Hsp92I GRCGYC 2 cut(s) 760, 1663
HspAI GCGC 3 cut(s) 392, 760, 775
KasI GGCGCC 1 cut(s) 759
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 7 cut(s) 43, 61, 155, 165, 1135, 1317, 1424
LmnI GCTCC 2 cut(s) 1022, 1035
Lsp1109I GCAGC 1 cut(s) 859
LweI GCATC 2 cut(s) 995, 1189
MaeI CTAG 2 cut(s) 195, 1019
MaeII ACGT 2 cut(s) 349, 422
MaeIII GTNAC 2 cut(s) 1079, 1627
MalI GATC 7 cut(s) 45, 63, 157, 167, 1137, 1319, 1426
MboI GATC 7 cut(s) 43, 61, 155, 165, 1135, 1317, 1424
MboII GAAGA 6 cut(s) 315, 821, 959, 1073, 1499, 1665
MfeI CAATTG 3 cut(s) 327, 1035, 1176
MflI RGATCY 1 cut(s) 1424
MhlI GDGCHC 3 cut(s) 347, 1032, 1651
MlsI TGGCCA 2 cut(s) 132, 1382
MluNI TGGCCA 2 cut(s) 132, 1382
Mly113I GGCGCC 1 cut(s) 760
MlyI GAGTC 2 cut(s) 947, 1295
MmeI TCCRAC 2 cut(s) 631, 1101
Mox20I TGGCCA 2 cut(s) 132, 1382
Mph1103I ATGCAT 1 cut(s) 1266
MscI TGGCCA 2 cut(s) 132, 1382
MseI TTAA 8 cut(s) 12, 570, 720, 767, 1254, 1442, 1497, 1599
MslI CAYNNNNRTG 1 cut(s) 768
Msp20I TGGCCA 2 cut(s) 132, 1382
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 1153
MunI CAATTG 3 cut(s) 327, 1035, 1176
MvaI CCWGG 1 cut(s) 1153
MvnI CGCG 2 cut(s) 394, 777
MwoI GCNNNNNNNGC 3 cut(s) 186, 195, 1004
NarI GGCGCC 1 cut(s) 760
NcoI CCATGG 2 cut(s) 1228, 1428
NdeI CATATG 1 cut(s) 1344
NdeII GATC 7 cut(s) 43, 61, 155, 165, 1135, 1317, 1424
NlaIV GGNNCC 4 cut(s) 416, 510, 761, 1426
NmuCI GTSAC 1 cut(s) 1079
NsiI ATGCAT 1 cut(s) 1266
NspI RCATGY 1 cut(s) 1628
PagI TCATGA 1 cut(s) 883
PfeI GAWTC 2 cut(s) 1556, 1670
PflFI GACNNNGTC 1 cut(s) 1285
PkrI GCNGC 1 cut(s) 874
PleI GAGTC 2 cut(s) 947, 1294
PluTI GGCGCC 1 cut(s) 763
PmaCI CACGTG 1 cut(s) 350
PmlI CACGTG 1 cut(s) 350
PpsI GAGTC 2 cut(s) 947, 1294
Ppu21I YACGTR 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 509
Psp124BI GAGCTC 2 cut(s) 347, 1032
Psp5II RGGWCCY 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 1151
PspCI CACGTG 1 cut(s) 350
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 1 cut(s) 1151
PspN4I GGNNCC 4 cut(s) 416, 510, 761, 1426
PspPI GGNCC 4 cut(s) 209, 332, 415, 509
PspPPI RGGWCCY 1 cut(s) 509
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 1 cut(s) 1424
PsyI GACNNNGTC 1 cut(s) 1285
RsaI GTAC 4 cut(s) 325, 534, 1330, 1437
RsaNI GTAC 4 cut(s) 324, 533, 1329, 1436
RseI CAYNNNNRTG 1 cut(s) 768
SacI GAGCTC 2 cut(s) 347, 1032
SaqAI TTAA 8 cut(s) 12, 570, 720, 767, 1254, 1442, 1497, 1599
SatI GCNGC 1 cut(s) 873
Sau3AI GATC 7 cut(s) 43, 61, 155, 165, 1135, 1317, 1424
Sau96I GGNCC 4 cut(s) 209, 332, 415, 509
SchI GAGTC 2 cut(s) 947, 1295
ScrFI CCNGG 1 cut(s) 1153
SduI GDGCHC 3 cut(s) 347, 1032, 1651
SfaNI GCATC 2 cut(s) 995, 1189
SfoI GGCGCC 1 cut(s) 761
SinI GGWCC 3 cut(s) 332, 415, 509
SmiI ATTTAAAT 1 cut(s) 768
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 1 cut(s) 1573
SmoI CTYRAG 1 cut(s) 1573
SsiI CCGC 3 cut(s) 777, 875, 984
SspDI GGCGCC 1 cut(s) 759
SspMI CTAG 2 cut(s) 195, 1019
SstI GAGCTC 2 cut(s) 347, 1032
StyD4I CCNGG 1 cut(s) 1151
StyI CCWWGG 4 cut(s) 504, 1018, 1228, 1428
SwaI ATTTAAAT 1 cut(s) 768
TaaI ACNGT 5 cut(s) 151, 610, 1085, 1270, 1388
TaiI ACGT 2 cut(s) 352, 425
TaqI TCGA 2 cut(s) 236, 301
TatI WGTACW 2 cut(s) 323, 1435
TfiI GAWTC 2 cut(s) 1556, 1670
Tru1I TTAA 8 cut(s) 12, 570, 720, 767, 1254, 1442, 1497, 1599
Tru9I TTAA 8 cut(s) 12, 570, 720, 767, 1254, 1442, 1497, 1599
TscAI CASTG 4 cut(s) 154, 165, 187, 1188
TseFI GTSAC 1 cut(s) 1079
TseI GCWGC 1 cut(s) 872
Tsp45I GTSAC 1 cut(s) 1079
TspDTI ATGAA 6 cut(s) 258, 398, 872, 914, 959, 1500
TspRI CASTG 4 cut(s) 154, 165, 187, 1188
Tth111I GACNNNGTC 1 cut(s) 1285
VneI GTGCAC 1 cut(s) 1647
VpaK11BI GGWCC 3 cut(s) 332, 415, 509
XapI RAATTY 2 cut(s) 92, 246
XceI RCATGY 1 cut(s) 1628
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmaJI CCTAGG 1 cut(s) 1018
XmiI GTMKAC 1 cut(s) 1294
XspI CTAG 2 cut(s) 195, 1019
Zsp2I ATGCAT 1 cut(s) 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.