MD06G1029200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016347
Physical Location & Seq
Reverse (-)
3487410 .. 3490451
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1029200.v1.1.491

Sequence Viewer

Length: 975 bp
ATGAAGATCTCCCTCAAGCTTCCAGAAGACCAACAAGAGTTCCAGTACCACCGCAACCCACTGATAAAGGCAAAGGTACCCGTAACCATATTCAACCAACCTTTTACTTCTTCCATTGCCACCATTTCATCCGCCGCCTCTGCCACCAAGTCTTCCTCTCACCTCTCATTTTCTCTCTCCTCCAACTTCTCTTCTGGCCCTTCCGTCAGACTCTCTTACTCTCACTCCTCCACCGCACCCTCCCCTTTCTCTCTCTCCCTCAAGTCTGGTCTTGGCCTCTTTGGCTCCCCAAAAAACTCCCCTCTTGTTTTTACTGCTCGCTTCTCTCTCTCCCACACAAACCCCACTTTCTCTCTCCACATCAAGCCCCAATTTGGCAACTTTTCACTCAAAAAATCCACCTTTTCCGACCCTAATTTCAAACAAATTTCTGGGTCTTCCTCCGATGTTGGTGACCATTTGGATTCTGGGTCTCCTTTAAATGGTGGTTTTGGTAATGGGCTTGTCTCTGAAGGGTCATCTGTTTGGCAGGAGCTGAGGTTGGAGCCTTCCGGCGCCAAAAATGGCGAATTTAGGGATAATTGTGGAGCTCATTCGAATGGCGGAATTGGGTCTGCGGCGGAGAGGTCATTGGGTTGGAAGAATGGTGGGAAAGATGGGTTGTTTTCTGGAATTGCTGTGATGGCAAGGACTGTGTTGCCGGTCACAAAAAGTTTGGTGGTGAATATGCGCTGGGGTGTGAATCTTCCTGCAAAGTTTGGTAAAACGATGCCTTATTTGACCGTGAATAAGATTGGTATTGAGAGAGTTGAGGAGGTGAAGATAGAGGAGGAAAAGAGTTCTGAGACCAATGTGGGTGACATGGAGTTGTTAAAGGGGATGTGCTTTTGGATGAGAAGGGATTTGGAGGTCTTGGAGAAGGAAAATAGGGAGATGAAGCAGAGGTTGGAGGATATTGCTGCCGCCTTCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

35.18

Weight (kDa)

9.1

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016347)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g21440
malus_domestica MD06G1029200.v1.1
prunus_persica Prupe.5G042700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0208871
rosa_laevigata RLG00000003188
rosa_multiflora Rmu_sc0000532.1_g000005 Rmu_sc0001482.1_g000003 Rmu_sc0016359.1_g000001
rosa_roxburghii Rroxscaffold_3G00250090
rosa_rugosa Rorug07G0107000
rosa_samantha Rh7AG242000 Rh7BG236800 Rh7CG256900 Rh7DG247900
rosa_wichuraiana Rw7G020490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 76
AccB1I GGYRCC 2 cut(s) 76, 554
AciI CCGC 8 cut(s) 52, 132, 135, 234, 603, 617, 620, 963
AcsI RAATTY 2 cut(s) 426, 569
AcuI CTGAAG 1 cut(s) 531
AcyI GRCGYC 1 cut(s) 555
AfaI GTAC 2 cut(s) 47, 78
AfiI CCNNNNNNNGG 2 cut(s) 374, 482
AgsI TTSAA 2 cut(s) 94, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 929, 961
AluBI AGCT 3 cut(s) 19, 535, 590
AluI AGCT 3 cut(s) 19, 535, 590
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 3 cut(s) 477, 511, 839
AlwNI CAGNNNCTG 1 cut(s) 535
AoxI GGCC 2 cut(s) 196, 274
ApeKI GCWGC 1 cut(s) 959
ApoI RAATTY 2 cut(s) 426, 569
Asp718I GGTACC 1 cut(s) 76
AspLEI GCGC 2 cut(s) 557, 732
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 5 cut(s) 152, 464, 733, 829, 869
AsuII TTCGAA 1 cut(s) 596
BanI GGYRCC 2 cut(s) 76, 554
BanII GRGCYC 1 cut(s) 592
BbsI GAAGAC 3 cut(s) 33, 144, 429
Bbv12I GWGCWC 1 cut(s) 592
BbvCI CCTCAGC 1 cut(s) 536
BbvI GCAGC 1 cut(s) 946
BccI CCATC 2 cut(s) 650, 676
BcoDI GTCTC 3 cut(s) 477, 511, 839
BfoI RGCGCY 1 cut(s) 558
BglI GCCNNNNNGGC 1 cut(s) 282
BglII AGATCT 1 cut(s) 6
BisI GCNGC 4 cut(s) 135, 618, 960, 963
BlsI GCNGC 4 cut(s) 136, 619, 961, 964
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 4 cut(s) 78, 286, 546, 556
BmsI GCATC 1 cut(s) 759
BpiI GAAGAC 3 cut(s) 33, 144, 429
Bpu10I CCTNAGC 1 cut(s) 536
Bpu14I TTCGAA 1 cut(s) 596
BpuEI CTTGAG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 555
BsaI GGTCTC 2 cut(s) 477, 839
BsaXI ACNNNNNCTCC 4 cut(s) 209, 239, 524, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 482
Bse118I RCCGGY 1 cut(s) 700
Bse1I ACTGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 114
BseGI GGATG 3 cut(s) 128, 885, 897
BseLI CCNNNNNNNGG 2 cut(s) 374, 482
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 2 cut(s) 527, 834
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 4 cut(s) 169, 217, 827, 842
BseXI GCAGC 1 cut(s) 946
BseYI CCCAGC 1 cut(s) 732
BshFI GGCC 2 cut(s) 198, 276
BshNI GGYRCC 2 cut(s) 76, 554
BsiHKAI GWGCWC 1 cut(s) 592
BsiSI CCGG 2 cut(s) 552, 701
BslI CCNNNNNNNGG 2 cut(s) 374, 482
BsmAI GTCTC 3 cut(s) 477, 511, 839
BsnI GGCC 2 cut(s) 198, 276
Bso31I GGTCTC 2 cut(s) 477, 839
Bsp119I TTCGAA 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 1 cut(s) 6
BspACI CCGC 8 cut(s) 52, 132, 135, 234, 603, 617, 620, 963
BspANI GGCC 2 cut(s) 198, 276
BspCNI CTCAG 2 cut(s) 528, 835
BspLI GGNNCC 4 cut(s) 78, 286, 546, 556
BspT104I TTCGAA 1 cut(s) 596
BspT107I GGYRCC 2 cut(s) 76, 554
BspTNI GGTCTC 2 cut(s) 477, 839
BsrDI GCAATG 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 700
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 700
BssMI GATC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 694, 784
Bst6I CTCTTC 1 cut(s) 196
BstACI GRCGYC 1 cut(s) 555
BstBI TTCGAA 1 cut(s) 596
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 2 cut(s) 536, 843
BstEII GGTNACC 1 cut(s) 452
BstF5I GGATG 3 cut(s) 128, 885, 897
BstH2I RGCGCY 1 cut(s) 558
BstHHI GCGC 2 cut(s) 557, 732
BstKTI GATC 1 cut(s) 9
BstMAI GTCTC 3 cut(s) 477, 511, 839
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 3 cut(s) 140, 282, 683
BstPI GGTNACC 1 cut(s) 452
BstV1I GCAGC 1 cut(s) 946
BstV2I GAAGAC 3 cut(s) 33, 144, 429
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 2 cut(s) 198, 276
BtsCI GGATG 3 cut(s) 128, 885, 897
BtsIMutI CAGTG 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 319
CaiI CAGNNNCTG 1 cut(s) 535
CfoI GCGC 2 cut(s) 557, 732
Cfr10I RCCGGY 1 cut(s) 700
Cfr13I GGNCC 1 cut(s) 197
Csp6I GTAC 2 cut(s) 46, 77
CviAII CATG 1 cut(s) 862
CviJI RGCY 9 cut(s) 19, 198, 276, 285, 367, 502, 535, 547, 590
CviKI_1 RGCY 9 cut(s) 19, 198, 276, 285, 367, 502, 535, 547, 590
CviQI GTAC 2 cut(s) 46, 77
DdeI CTNAG 2 cut(s) 536, 843
DinI GGCGCC 1 cut(s) 556
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
DraI TTTAAA 1 cut(s) 480
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
EciI GGCGGA 3 cut(s) 121, 618, 635
Ecl136II GAGCTC 1 cut(s) 590
Eco24I GRGCYC 1 cut(s) 592
Eco31I GGTCTC 2 cut(s) 477, 839
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 531
Eco91I GGTNACC 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 590
EcoO65I GGTNACC 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 592
EgeI GGCGCC 1 cut(s) 556
EheI GGCGCC 1 cut(s) 556
FaeI CATG 1 cut(s) 865
FaiI YATR 3 cut(s) 89, 728, 863
FatI CATG 1 cut(s) 861
Fnu4HI GCNGC 4 cut(s) 135, 618, 960, 963
FokI GGATG 3 cut(s) 115, 892, 904
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 4 cut(s) 135, 618, 960, 963
GlaI GCGC 2 cut(s) 556, 731
GluI GCNGC 4 cut(s) 135, 618, 960, 963
GsaI CCCAGC 1 cut(s) 736
HaeII RGCGCY 1 cut(s) 558
HaeIII GGCC 2 cut(s) 198, 276
HapII CCGG 2 cut(s) 552, 701
HhaI GCGC 2 cut(s) 557, 732
Hin1I GRCGYC 1 cut(s) 555
Hin1II CATG 1 cut(s) 865
Hin6I GCGC 2 cut(s) 555, 730
HinP1I GCGC 2 cut(s) 555, 730
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 3 cut(s) 210, 464, 742
HpaII CCGG 2 cut(s) 552, 701
HphI GGTGA 5 cut(s) 152, 464, 733, 829, 869
Hpy188I TCNGA 5 cut(s) 209, 409, 445, 511, 844
Hpy188III TCNNGA 2 cut(s) 23, 669
HpyAV CCTTC 5 cut(s) 210, 506, 558, 891, 913
HpyCH4III ACNGT 2 cut(s) 694, 784
HpyCH4V TGCA 1 cut(s) 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 282, 683
HpyF3I CTNAG 2 cut(s) 536, 843
Hsp92I GRCGYC 1 cut(s) 555
Hsp92II CATG 1 cut(s) 865
HspAI GCGC 2 cut(s) 555, 730
KasI GGCGCC 1 cut(s) 554
KpnI GGTACC 1 cut(s) 80
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 4 cut(s) 290, 532, 544, 587
Lsp1109I GCAGC 1 cut(s) 946
LweI GCATC 1 cut(s) 759
MaeIII GTNAC 4 cut(s) 82, 452, 703, 857
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 9 cut(s) 16, 38, 102, 144, 183, 429, 652, 737, 832
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 7 cut(s) 371, 415, 426, 569, 580, 606, 672
Mly113I GGCGCC 1 cut(s) 555
MlyI GAGTC 1 cut(s) 204
MmeI TCCRAC 5 cut(s) 207, 432, 522, 617, 927
MseI TTAA 2 cut(s) 479, 872
MslI CAYNNNNRTG 1 cut(s) 597
MspI CCGG 2 cut(s) 552, 701
MwoI GCNNNNNNNGC 3 cut(s) 140, 282, 683
NarI GGCGCC 1 cut(s) 555
NdeII GATC 1 cut(s) 6
NlaIII CATG 1 cut(s) 865
NlaIV GGNNCC 4 cut(s) 78, 286, 546, 556
NmuCI GTSAC 3 cut(s) 452, 703, 857
NspV TTCGAA 1 cut(s) 596
PfeI GAWTC 2 cut(s) 464, 742
PkrI GCNGC 4 cut(s) 136, 619, 961, 964
PleI GAGTC 1 cut(s) 204
PluTI GGCGCC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 592
PspEI GGTNACC 1 cut(s) 452
PspFI CCCAGC 1 cut(s) 732
PspN4I GGNNCC 4 cut(s) 78, 286, 546, 556
PspPI GGNCC 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 535
PsuI RGATCY 1 cut(s) 6
RsaI GTAC 2 cut(s) 47, 78
RsaNI GTAC 2 cut(s) 46, 77
RseI CAYNNNNRTG 1 cut(s) 597
SacI GAGCTC 1 cut(s) 592
SaqAI TTAA 2 cut(s) 479, 872
SatI GCNGC 4 cut(s) 135, 618, 960, 963
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 197
SchI GAGTC 1 cut(s) 204
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 1 cut(s) 759
SfoI GGCGCC 1 cut(s) 556
SfuI TTCGAA 1 cut(s) 596
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 2 cut(s) 14, 260
SmoI CTYRAG 2 cut(s) 14, 260
Sse9I AATT 7 cut(s) 371, 415, 426, 569, 580, 606, 672
SsiI CCGC 8 cut(s) 52, 132, 135, 234, 603, 617, 620, 963
SspDI GGCGCC 1 cut(s) 554
SstI GAGCTC 1 cut(s) 592
TaaI ACNGT 2 cut(s) 694, 784
TaqI TCGA 2 cut(s) 596, 969
TasI AATT 7 cut(s) 371, 415, 426, 569, 580, 606, 672
TauI GCSGC 3 cut(s) 137, 620, 965
TfiI GAWTC 2 cut(s) 464, 742
Tru1I TTAA 2 cut(s) 479, 872
Tru9I TTAA 2 cut(s) 479, 872
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 3 cut(s) 452, 703, 857
TseI GCWGC 1 cut(s) 959
Tsp45I GTSAC 3 cut(s) 452, 703, 857
TspDTI ATGAA 3 cut(s) 17, 117, 950
TspGWI ACGGA 1 cut(s) 193
TspRI CASTG 1 cut(s) 66
XapI RAATTY 2 cut(s) 426, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.