FvH4_5g22621

Ubiquitin-2 like Rad60 SUMO-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017474
Physical Location & Seq
Forward (+)
13991727 .. 13993977
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22621.t1

Sequence Viewer

Length: 549 bp
ATGCAGATATTCGTGAAAACCCTAACGGGCAAGACCATCACCCTCGAGGTGGAGAGCTCCGACACCATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAGGGCATCCCCCCAGACCAGCAGAGGCTCATCTTCGCTGGCAAGCAGTTGGAGGATGGCCGCACTCTCGCAGATTATAACATCCAGAAGGAGTCTACACTCCACCTTGTCCTTCGTCTCCGTGGTGGTGATCATATAATGAATCAAGGCAGTGTAGTTTCTCGCGGAAACAACGAGACCAGTATTACAGGGAATGCAGCAAGGCATACCTCTTGTGATGCTATTGCTGCAATAACAGACTCTGAGAATTATAGATTTCTAGACATGGGTCGGCGGAGAAGCGTTTGTTCTAGGCGTCTTGCTGAAAAGTCTTGTGTGGTGCGCCGTGCGCGAGAAGTGAGCATGGAGTTGATCATGAACTTATTACCACTACCAATGCCATGCTGTGCTTCATGTTGTCACCACTCACCACCACAGATGAAAACCCCTCTTCTAGCTCGCTCCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

183

Amino Acids

20.24

Weight (kDa)

8.92

Isoelectric Point (pI)

55.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 2.1e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 2.7e-32 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11980
fragaria_vesca FvH4_5g22621
malus_domestica MD07G1147200.v1.1
rosa_chinensis RchiOBHm_Chr3g0478011 RchiOBHm_Chr3g0478541 RchiOBHm_Chr3g0483741 RchiOBHm_Chr4g0426441
rosa_roxburghii Rroxscaffold_6G00403310
rosa_samantha Rh3AG215800 Rh3AG256600 Rh3BG173200 Rh3BG302700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AbsI CCTCGAGG 1 cut(s) 44
AccI GTMKAC 1 cut(s) 194
AccII CGCG 2 cut(s) 264, 430
AciI CCGC 3 cut(s) 160, 264, 373
AclWI GGATC 1 cut(s) 82
AcoI YGGCCR 1 cut(s) 157
AcyI GRCGYC 1 cut(s) 394
AfiI CCNNNNNNNGG 2 cut(s) 49, 97
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 2 cut(s) 57, 536
AluI AGCT 2 cut(s) 57, 536
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 2 cut(s) 221, 269
AlwI GGATC 1 cut(s) 82
AlwNI CAGNNNCTG 1 cut(s) 341
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 2 cut(s) 81, 157
ApeKI GCWGC 2 cut(s) 296, 326
AspLEI GCGC 2 cut(s) 423, 430
AsuHPI GGTGA 4 cut(s) 31, 239, 491, 498
AvaI CYCGRG 1 cut(s) 44
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 2 cut(s) 308, 313
BccI CCATC 3 cut(s) 44, 74, 149
BceAI ACGGC 1 cut(s) 408
BciT130I CCWGG 1 cut(s) 92
BclI TGATCA 2 cut(s) 229, 450
BcoDI GTCTC 2 cut(s) 221, 269
BfaI CTAG 3 cut(s) 359, 390, 533
BisI GCNGC 3 cut(s) 160, 297, 327
BlsI GCNGC 3 cut(s) 161, 298, 328
Bme1390I CCNGG 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 44
BmrFI CCNGG 1 cut(s) 92
BmsI GCATC 2 cut(s) 114, 307
BoxI GACNNNNGTC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 394
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 220
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 97
Bse1I ACTGG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 3 cut(s) 105, 160, 180
BseLI CCNNNNNNNGG 2 cut(s) 49, 97
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 308, 313
Bsh1236I CGCG 2 cut(s) 264, 430
BshFI GGCC 2 cut(s) 83, 159
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 44
BslI CCNNNNNNNGG 2 cut(s) 49, 97
BsmAI GTCTC 2 cut(s) 221, 269
BsmBI CGTCTC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 2 cut(s) 83, 159
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 3 cut(s) 87, 229, 450
BspACI CCGC 3 cut(s) 160, 264, 373
BspANI GGCC 2 cut(s) 83, 159
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 2 cut(s) 264, 430
BspHI TCATGA 1 cut(s) 453
BspPI GGATC 1 cut(s) 82
BspTNI GGTCTC 1 cut(s) 269
BsrI ACTGG 1 cut(s) 279
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 3 cut(s) 87, 229, 450
BssNI GRCGYC 1 cut(s) 394
Bst2UI CCWGG 1 cut(s) 92
Bst6I CTCTTC 1 cut(s) 534
BstACI GRCGYC 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 139, 143, 538
BstDEI CTNAG 1 cut(s) 342
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 3 cut(s) 105, 160, 180
BstFNI CGCG 2 cut(s) 264, 430
BstHHI GCGC 2 cut(s) 423, 430
BstKTI GATC 3 cut(s) 90, 232, 453
BstMAI GTCTC 2 cut(s) 221, 269
BstMBI GATC 3 cut(s) 87, 229, 450
BstMWI GCNNNNNNNGC 2 cut(s) 326, 427
BstNI CCWGG 1 cut(s) 92
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 90
BstUI CGCG 2 cut(s) 264, 430
BstV1I GCAGC 2 cut(s) 308, 313
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BsuRI GGCC 2 cut(s) 83, 159
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 3 cut(s) 105, 160, 180
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 3 cut(s) 139, 143, 538
CaiI CAGNNNCTG 1 cut(s) 341
CciI TCATGA 1 cut(s) 453
CfoI GCGC 2 cut(s) 423, 430
CseI GACGC 1 cut(s) 383
CviAII CATG 5 cut(s) 364, 442, 454, 480, 492
CviJI RGCY 5 cut(s) 57, 83, 127, 159, 536
CviKI_1 RGCY 5 cut(s) 57, 83, 127, 159, 536
DdeI CTNAG 1 cut(s) 342
DpnI GATC 3 cut(s) 89, 231, 452
DpnII GATC 3 cut(s) 87, 229, 450
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
EciI GGCGGA 1 cut(s) 388
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 269
Eco53kI GAGCTC 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 59
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 5 cut(s) 367, 445, 457, 483, 495
FatI CATG 5 cut(s) 363, 441, 453, 479, 491
FbaI TGATCA 2 cut(s) 229, 450
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 3 cut(s) 160, 297, 327
FokI GGATG 3 cut(s) 92, 167, 167
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 3 cut(s) 160, 297, 327
FspBI CTAG 3 cut(s) 359, 390, 533
GlaI GCGC 2 cut(s) 422, 429
GluI GCNGC 3 cut(s) 160, 297, 327
HaeIII GGCC 2 cut(s) 83, 159
HgaI GACGC 1 cut(s) 383
HhaI GCGC 2 cut(s) 423, 430
Hin1I GRCGYC 1 cut(s) 394
Hin1II CATG 5 cut(s) 367, 445, 457, 483, 495
Hin6I GCGC 2 cut(s) 421, 428
HinP1I GCGC 2 cut(s) 421, 428
HinfI GANTC 3 cut(s) 191, 241, 338
HphI GGTGA 4 cut(s) 31, 239, 491, 498
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 2 cut(s) 61, 343
Hpy188III TCNNGA 4 cut(s) 13, 184, 359, 454
Hpy8I GTNNAC 1 cut(s) 195
HpyAV CCTTC 2 cut(s) 181, 221
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 3 cut(s) 4, 296, 329
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 427
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 394
Hsp92II CATG 5 cut(s) 367, 445, 457, 483, 495
HspAI GCGC 2 cut(s) 421, 428
Ksp22I TGATCA 2 cut(s) 229, 450
Kzo9I GATC 3 cut(s) 87, 229, 450
LmnI GCTCC 2 cut(s) 62, 545
LpnPI CCDG 8 cut(s) 77, 104, 123, 126, 131, 197, 273, 292
Lsp1109I GCAGC 2 cut(s) 308, 313
LweI GCATC 2 cut(s) 114, 307
MaeI CTAG 3 cut(s) 359, 390, 533
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 1 cut(s) 497
MalI GATC 3 cut(s) 89, 231, 452
MboI GATC 3 cut(s) 87, 229, 450
MboII GAAGA 2 cut(s) 124, 521
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 1 cut(s) 346
MlyI GAGTC 2 cut(s) 200, 332
MmeI TCCRAC 2 cut(s) 84, 129
MnlI CCTC 7 cut(s) 40, 53, 94, 117, 145, 319, 537
MspR9I CCNGG 1 cut(s) 92
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 1 cut(s) 92
MvnI CGCG 2 cut(s) 264, 430
MwoI GCNNNNNNNGC 2 cut(s) 326, 427
NdeII GATC 3 cut(s) 87, 229, 450
NlaIII CATG 5 cut(s) 367, 445, 457, 483, 495
NmuCI GTSAC 1 cut(s) 497
PaeR7I CTCGAG 1 cut(s) 44
PagI TCATGA 1 cut(s) 453
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 1 cut(s) 241
PflFI GACNNNGTC 1 cut(s) 74
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 3 cut(s) 161, 298, 328
PleI GAGTC 2 cut(s) 199, 332
PpsI GAGTC 2 cut(s) 199, 332
PshAI GACNNNNGTC 1 cut(s) 366
PsiI TTATAA 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspXI VCTCGAGB 1 cut(s) 44
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 87
PsyI GACNNNGTC 1 cut(s) 74
SacI GAGCTC 1 cut(s) 59
SatI GCNGC 3 cut(s) 160, 297, 327
Sau3AI GATC 3 cut(s) 87, 229, 450
SchI GAGTC 2 cut(s) 200, 332
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 1 cut(s) 59
SetI ASST 6 cut(s) 51, 59, 78, 207, 311, 538
SfaNI GCATC 2 cut(s) 114, 307
Sfr274I CTCGAG 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 1 cut(s) 346
SsiI CCGC 3 cut(s) 160, 264, 373
SspMI CTAG 3 cut(s) 359, 390, 533
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 90
TaiI ACGT 1 cut(s) 78
TaqI TCGA 2 cut(s) 45, 69
TasI AATT 1 cut(s) 346
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 241
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 2 cut(s) 296, 326
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 4 cut(s) 254, 470, 480, 533
TspGWI ACGGA 2 cut(s) 209, 532
TspRI CASTG 1 cut(s) 256
Tth111I GACNNNGTC 1 cut(s) 74
XbaI TCTAGA 1 cut(s) 358
XhoI CTCGAG 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 194
XspI CTAG 3 cut(s) 359, 390, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.