Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G11980 |
| fragaria_vesca | FvH4_5g22621 |
| malus_domestica | MD07G1147200.v1.1 |
| rosa_chinensis | RchiOBHm_Chr3g0478011 RchiOBHm_Chr3g0478541 RchiOBHm_Chr3g0483741 RchiOBHm_Chr4g0426441 |
| rosa_roxburghii | Rroxscaffold_6G00403310 |
| rosa_samantha | Rh3AG215800 Rh3AG256600 Rh3BG173200 Rh3BG302700 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 110 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 27, 49 |
| AgsI | TTSAA | 1 cut(s) | 92 |
| AjnI | CCWGG | 1 cut(s) | 42 |
| AluBI | AGCT | 1 cut(s) | 148 |
| AluI | AGCT | 1 cut(s) | 148 |
| Ama87I | CYCGRG | 1 cut(s) | 149 |
| AoxI | GGCC | 1 cut(s) | 81 |
| ApeKI | GCWGC | 1 cut(s) | 145 |
| AspS9I | GGNCC | 1 cut(s) | 115 |
| AsuHPI | GGTGA | 1 cut(s) | 31 |
| AvaI | CYCGRG | 1 cut(s) | 149 |
| AvaII | GGWCC | 1 cut(s) | 115 |
| BbsI | GAAGAC | 1 cut(s) | 38 |
| BbvI | GCAGC | 1 cut(s) | 157 |
| BccI | CCATC | 1 cut(s) | 74 |
| BciT130I | CCWGG | 1 cut(s) | 44 |
| BglII | AGATCT | 1 cut(s) | 6 |
| BisI | GCNGC | 1 cut(s) | 146 |
| BlsI | GCNGC | 1 cut(s) | 147 |
| Bme1390I | CCNGG | 1 cut(s) | 44 |
| Bme18I | GGWCC | 1 cut(s) | 115 |
| BmeT110I | CYCGRG | 1 cut(s) | 149 |
| BmgT120I | GGNCC | 1 cut(s) | 115 |
| BmrFI | CCNGG | 1 cut(s) | 44 |
| BmsI | GCATC | 1 cut(s) | 114 |
| BpiI | GAAGAC | 1 cut(s) | 38 |
| BpmI | CTGGAG | 1 cut(s) | 65 |
| BsaJI | CCNNGG | 1 cut(s) | 42 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 38, 68 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 27, 49 |
| BseBI | CCWGG | 1 cut(s) | 44 |
| BseDI | CCNNGG | 1 cut(s) | 42 |
| BseGI | GGATG | 1 cut(s) | 105 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 27, 49 |
| BseXI | GCAGC | 1 cut(s) | 157 |
| BshFI | GGCC | 1 cut(s) | 83 |
| BsiHKCI | CYCGRG | 1 cut(s) | 149 |
| BsiSI | CCGG | 2 cut(s) | 113, 138 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 27, 49 |
| BsnI | GGCC | 1 cut(s) | 83 |
| BsoBI | CYCGRG | 1 cut(s) | 149 |
| Bsp143I | GATC | 2 cut(s) | 6, 36 |
| BspACI | CCGC | 1 cut(s) | 110 |
| BspANI | GGCC | 1 cut(s) | 83 |
| BssECI | CCNNGG | 1 cut(s) | 42 |
| BssMI | GATC | 2 cut(s) | 6, 36 |
| Bst2UI | CCWGG | 1 cut(s) | 44 |
| Bst4CI | ACNGT | 1 cut(s) | 22 |
| BstF5I | GGATG | 1 cut(s) | 105 |
| BstKTI | GATC | 2 cut(s) | 9, 39 |
| BstMBI | GATC | 2 cut(s) | 6, 36 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 118 |
| BstNI | CCWGG | 1 cut(s) | 44 |
| BstSCI | CCNGG | 1 cut(s) | 42 |
| BstV1I | GCAGC | 1 cut(s) | 157 |
| BstV2I | GAAGAC | 1 cut(s) | 38 |
| BstX2I | RGATCY | 1 cut(s) | 6 |
| BstYI | RGATCY | 1 cut(s) | 6 |
| BsuRI | GGCC | 1 cut(s) | 83 |
| BtsCI | GGATG | 1 cut(s) | 105 |
| Cfr13I | GGNCC | 1 cut(s) | 115 |
| CviJI | RGCY | 4 cut(s) | 83, 127, 148, 159 |
| CviKI_1 | RGCY | 4 cut(s) | 83, 127, 148, 159 |
| DpnI | GATC | 2 cut(s) | 8, 38 |
| DpnII | GATC | 2 cut(s) | 6, 36 |
| Eco47I | GGWCC | 1 cut(s) | 115 |
| Eco88I | CYCGRG | 1 cut(s) | 149 |
| EcoRII | CCWGG | 1 cut(s) | 42 |
| FauI | CCCGC | 1 cut(s) | 117 |
| Fnu4HI | GCNGC | 1 cut(s) | 146 |
| FokI | GGATG | 1 cut(s) | 92 |
| Fsp4HI | GCNGC | 1 cut(s) | 146 |
| GluI | GCNGC | 1 cut(s) | 146 |
| GsuI | CTGGAG | 1 cut(s) | 65 |
| HaeIII | GGCC | 1 cut(s) | 83 |
| HapII | CCGG | 2 cut(s) | 113, 138 |
| HinfI | GANTC | 2 cut(s) | 53, 88 |
| HpaII | CCGG | 2 cut(s) | 113, 138 |
| HphI | GGTGA | 1 cut(s) | 31 |
| Hpy188I | TCNGA | 1 cut(s) | 61 |
| Hpy188III | TCNNGA | 2 cut(s) | 13, 92 |
| Hpy99I | CGWCG | 1 cut(s) | 61 |
| HpyAV | CCTTC | 1 cut(s) | 73 |
| HpyCH4III | ACNGT | 1 cut(s) | 22 |
| HpyCH4IV | ACGT | 1 cut(s) | 75 |
| HpyCH4V | TGCA | 1 cut(s) | 4 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 118 |
| HpySE526I | ACGT | 1 cut(s) | 75 |
| Kzo9I | GATC | 2 cut(s) | 6, 36 |
| LpnPI | CCDG | 5 cut(s) | 29, 56, 126, 131, 151 |
| Lsp1109I | GCAGC | 1 cut(s) | 157 |
| LweI | GCATC | 1 cut(s) | 114 |
| MaeII | ACGT | 1 cut(s) | 75 |
| MaeIII | GTNAC | 1 cut(s) | 22 |
| MalI | GATC | 2 cut(s) | 8, 38 |
| MboI | GATC | 2 cut(s) | 6, 36 |
| MboII | GAAGA | 2 cut(s) | 43, 124 |
| MflI | RGATCY | 1 cut(s) | 6 |
| MlyI | GAGTC | 1 cut(s) | 62 |
| MmeI | TCCRAC | 1 cut(s) | 39 |
| MnlI | CCTC | 5 cut(s) | 21, 40, 94, 117, 145 |
| MspI | CCGG | 2 cut(s) | 113, 138 |
| MspR9I | CCNGG | 1 cut(s) | 44 |
| MvaI | CCWGG | 1 cut(s) | 44 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 118 |
| NdeII | GATC | 2 cut(s) | 6, 36 |
| PaeR7I | CTCGAG | 1 cut(s) | 149 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 18, 32 |
| PfeI | GAWTC | 1 cut(s) | 88 |
| PkrI | GCNGC | 1 cut(s) | 147 |
| PleI | GAGTC | 1 cut(s) | 61 |
| PpsI | GAGTC | 1 cut(s) | 61 |
| Psp6I | CCWGG | 1 cut(s) | 42 |
| PspGI | CCWGG | 1 cut(s) | 42 |
| PspPI | GGNCC | 1 cut(s) | 115 |
| PspXI | VCTCGAGB | 1 cut(s) | 149 |
| PsuI | RGATCY | 1 cut(s) | 6 |
| SatI | GCNGC | 1 cut(s) | 146 |
| Sau3AI | GATC | 2 cut(s) | 6, 36 |
| Sau96I | GGNCC | 1 cut(s) | 115 |
| SchI | GAGTC | 1 cut(s) | 62 |
| ScrFI | CCNGG | 1 cut(s) | 44 |
| SetI | ASST | 3 cut(s) | 51, 78, 150 |
| SfaNI | GCATC | 1 cut(s) | 114 |
| Sfr274I | CTCGAG | 1 cut(s) | 149 |
| SinI | GGWCC | 1 cut(s) | 115 |
| SlaI | CTCGAG | 1 cut(s) | 149 |
| SmlI | CTYRAG | 1 cut(s) | 149 |
| SmoI | CTYRAG | 1 cut(s) | 149 |
| SsiI | CCGC | 1 cut(s) | 110 |
| StyD4I | CCNGG | 1 cut(s) | 42 |
| TaaI | ACNGT | 1 cut(s) | 22 |
| TaiI | ACGT | 1 cut(s) | 78 |
| TaqI | TCGA | 2 cut(s) | 69, 150 |
| TfiI | GAWTC | 1 cut(s) | 88 |
| TseI | GCWGC | 1 cut(s) | 145 |
| VpaK11BI | GGWCC | 1 cut(s) | 115 |
| XhoI | CTCGAG | 1 cut(s) | 149 |