FvH4_5g27530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003419
Physical Location & Seq
Reverse (-)
18778986 .. 18779672
687 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27530.t1

Sequence Viewer

Length: 267 bp
ATGACGTCGATACGCGCCACCGTGGACTTACATCTGCAGTTAATAATATTATTTCCTTTTAGTGTTTGGTTTAATACTAGGAATCTTGGTCCTAAGCTCGCATTACCTTCTTATTGGTGTTGCAGATCATTAAATGCAATGGATCCTGGAAAGCTGGGAGAGATATTGAAGCACTTGGAGATGCAGAACGAGCTCCTGACGAAAGCGTCTGAATCAATGATACAAGAATTGTCTAAGCTTCGGGTTGTTTCTTTAAAACTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

89

Amino Acids

10.06

Weight (kDa)

9.14

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 205
AatII GACGTC 1 cut(s) 8
AccII CGCG 1 cut(s) 15
AclWI GGATC 2 cut(s) 137, 150
AcyI GRCGYC 1 cut(s) 5
AgsI TTSAA 1 cut(s) 169
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 4 cut(s) 97, 154, 193, 238
AluI AGCT 4 cut(s) 97, 154, 193, 238
Alw21I GWGCWC 1 cut(s) 195
AlwI GGATC 2 cut(s) 137, 150
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 89
AvaII GGWCC 1 cut(s) 89
BamHI GGATCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BciT130I CCWGG 1 cut(s) 147
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 35
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 21
BseMI GCAATG 1 cut(s) 144
BseYI CCCAGC 1 cut(s) 154
Bsh1236I CGCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 195
Bsp143I GATC 2 cut(s) 125, 142
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 144
BspMAI CTGCAG 1 cut(s) 39
BspPI GGATC 2 cut(s) 137, 150
BsrDI GCAATG 1 cut(s) 144
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 2 cut(s) 125, 142
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 2 cut(s) 93, 234
BstDSI CCRYGG 1 cut(s) 21
BstFNI CGCG 1 cut(s) 15
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 2 cut(s) 128, 145
BstMBI GATC 2 cut(s) 125, 142
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 1 cut(s) 35
BstUI CGCG 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BtgI CCRYGG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 99
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 89
CseI GACGC 1 cut(s) 195
CviJI RGCY 4 cut(s) 97, 154, 193, 238
CviKI_1 RGCY 4 cut(s) 97, 154, 193, 238
DdeI CTNAG 2 cut(s) 93, 234
DpnI GATC 2 cut(s) 127, 144
DpnII GATC 2 cut(s) 125, 142
DraI TTTAAA 1 cut(s) 255
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 195
FriOI GRGCYC 1 cut(s) 195
FspBI CTAG 1 cut(s) 78
GlaI GCGC 1 cut(s) 16
GsaI CCCAGC 1 cut(s) 158
HgaI GACGC 1 cut(s) 195
HhaI GCGC 1 cut(s) 17
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 2 cut(s) 82, 212
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 117
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 4 cut(s) 37, 123, 137, 184
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 93, 234
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 15
Kzo9I GATC 2 cut(s) 125, 142
LmnI GCTCC 1 cut(s) 198
LpnPI CCDG 4 cut(s) 132, 140, 159, 209
LweI GCATC 1 cut(s) 171
MaeI CTAG 1 cut(s) 78
MaeII ACGT 1 cut(s) 5
MalI GATC 2 cut(s) 127, 144
MboI GATC 2 cut(s) 125, 142
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 2 cut(s) 227, 262
MseI TTAA 4 cut(s) 41, 72, 131, 254
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 190
NdeII GATC 2 cut(s) 125, 142
NlaIV GGNNCC 1 cut(s) 144
PfeI GAWTC 2 cut(s) 82, 212
PfoI TCCNGGA 1 cut(s) 145
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 89
PstI CTGCAG 1 cut(s) 39
PsuI RGATCY 1 cut(s) 142
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 4 cut(s) 41, 72, 131, 254
Sau3AI GATC 2 cut(s) 125, 142
Sau96I GGNCC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 195
SetI ASST 6 cut(s) 8, 99, 109, 156, 195, 240
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 1 cut(s) 35
SinI GGWCC 1 cut(s) 89
Sse9I AATT 2 cut(s) 227, 262
SspI AATATT 1 cut(s) 48
SspMI CTAG 1 cut(s) 78
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 1 cut(s) 8
TaqI TCGA 1 cut(s) 8
TasI AATT 2 cut(s) 227, 262
TfiI GAWTC 2 cut(s) 82, 212
Tru1I TTAA 4 cut(s) 41, 72, 131, 254
Tru9I TTAA 4 cut(s) 41, 72, 131, 254
VpaK11BI GGWCC 1 cut(s) 89
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.