Rorug02G0146400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003419
Physical Location & Seq
Reverse (-)
12857881 .. 12860776
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0146400.1

Sequence Viewer

Length: 1854 bp
ATGGCTTACATGTGTACAGACAGCGGGAATCTAATGGCGATTGCTCAGCAAGTCATCAAGCAAAAGCAGCAGCAAGAGCAACAGCAGCAGCAGCACACCACCCACCACCACCACCAGCAGCTCACTACGGCGTTCTCTCTCTCCTCTGCTCCATGGCCCACCAGCACTCATCACGCTCCGAGTCTGGGGTACGCGTTGGGGGGTTCGAGTTTTTCCGACCCGTTTCAGCTGCCCGGGAGCTCCGACACGACGGTCGGGGAGCCCGGGTTTCCGTTCCCGAACATGGATCATCATCCGACCAGCAGTGGGTTCAGGTTCTCGGATTTGGGCGGAGCGGCCGAGTTCGACTCGGACGAGTGGATGGACAGTATAATAGGCGGTGGGGATTCAACGGATAGTTCGAACCTGCCCTCTGTGTGCGACGCGTGGCAGAACAATGCCGCGGATTTCGGTCTCTACGCCTCCGATCCCTTCGCCCCCTGCCCGAGTCGACTCAGCGCTCCGTGCTCTTCGCCGTCCGATCTCAACCGAGTCATATACACCGACGTTCACAACCAGAAAACAGCCAATCTCCAACCTCAATTGCCGCCAGCGTGGACCCCATCTCCACCTCCGCCTCCGCCGCAGCAGCCTTCCTCAATTGCCGTTAAAGACGCCAAATCAGCCAACCCTACTCCTCAAAACAACGACGCCGTCGGAGTGTCCTCCAGGTCGCCGGAGATCGAATCGGCGCCGCCGCTACTGAAGGCCCTACTCGACTGCGCCAGAGTCATCGAGTCCGAACCGGAGCGTGCGATCAAATCGCTGGTCCGGCTCAGAGAGTCCGTGTCCGAGCACGGAGATCCAACCGAGCGAGTCGCTTTCCACTTCACGGACGCTCTGTACAGCCGAGTCTCTCCGCCGCAACCGGAGAAAAGCTCCCCCGCCTCGTACGACGCTAGCTGCGAAGAAGACTTCACTCTCTCTTATAAGGCCTTGAACGACGCTTGCCCTTACTCCAAGTTTGCTCACTTGACGGCGAACCAGGCCATCCTCGAAGCGACGGAGAGGGCGACGAAGATTCACATAGTCGACTTCGGGATAGTCCAAGGTGTCCAATGGGCCGCTCTATTGCAGGCTTTGGCTACCCGGTCCACCGGAAAGCCCAGAAAAATTCGGATCTCCGGTATACCCGCTCCGTCTCTGGGGAACTCTCCGGCGGCGTCGCTTTTCGCTACTGGAAACAGACTGTGTGAATTTGCAAAGCTTCTGGAGCTGAATTTCGAGTTTGAACCGGTGCTGACTCCGATTCAGGAGCTTAACGAGTCGTGCTTTCGGGTCGACCCGGATGAGTTCCTGGCGGTGAACTTAATGCTCCAGTTGCACAACCTCCTCGACGAATCAACCACCGGAGTTGAAGCGGCTCTGAGACTGGCCAAGTCGCTGAACCCGAAGATTGTGACCCTGGGCGAGTACGAAGCGAAATTGAACCGGGTCGGGTTCATGAGCCGGTTCGAGACCGCATTGAAGTACTACACAGCAATGTTTGAGTCACTTGAACCGAACATGACCCGAGACTCGCCGGAGAGGCTGCAGGTGGAGAGGCTTTTGCTGGGCCGGAGAATTGCTGGCGGGGTCGGCCCAGAGGAACCGGGAACGAAACGACAGCGTTTGGAGGACAAAGAGCAGTGGAAGTTTTTGATGGAAAGCTCTGGTTTTGAGCCAGTAACTCTTAGCCATTACGCAGTTAGCCAGGCCAAGATTCTTCTCTGGAACTATAGTAATGCTTCGTATTCGCTAAACGAATCTTCACCGGGGTTTCTCTCTTTGGCGTGGAAGGAAGTCCCTCTCTTTACTGTTTCTTCATGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

617

Amino Acids

67.69

Weight (kDa)

5.29

Isoelectric Point (pI)

60.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 248 - 617 2.8e-110 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 969
AarI CACCTGC 1 cut(s) 1564
AasI GACNNNNNNGTC 1 cut(s) 251
Acc36I ACCTGC 2 cut(s) 414, 1564
AccB1I GGYRCC 1 cut(s) 730
AccBSI CCGCTC 3 cut(s) 335, 1106, 1175
AccI GTMKAC 4 cut(s) 490, 1071, 1168, 1320
AccII CGCG 3 cut(s) 194, 425, 443
AclWI GGATC 4 cut(s) 294, 461, 836, 1166
AcoI YGGCCR 2 cut(s) 336, 1413
AcsI RAATTY 3 cut(s) 1152, 1235, 1258
AcuI CTGAAG 1 cut(s) 764
AcyI GRCGYC 4 cut(s) 654, 690, 731, 1202
AfaI GTAC 6 cut(s) 16, 191, 884, 932, 1454, 1511
AfeI AGCGCT 1 cut(s) 499
AfiI CCNNNNNNNGG 5 cut(s) 185, 283, 306, 871, 1184
AflIII ACRYGT 3 cut(s) 9, 192, 423
AgeI ACCGGT 1 cut(s) 1273
AgsI TTSAA 7 cut(s) 390, 979, 1271, 1397, 1468, 1507, 1538
AjnI CCWGG 5 cut(s) 707, 1023, 1335, 1443, 1731
AluBI AGCT 9 cut(s) 121, 229, 240, 918, 942, 1246, 1255, 1297, 1689
AluI AGCT 9 cut(s) 121, 229, 240, 918, 942, 1246, 1255, 1297, 1689
Alw21I GWGCWC 3 cut(s) 242, 509, 837
Alw26I GTCTC 6 cut(s) 458, 898, 1185, 1402, 1490, 1548
AlwI GGATC 4 cut(s) 294, 461, 836, 1166
Ama87I CYCGRG 4 cut(s) 233, 263, 484, 1551
Aor51HI AGCGCT 1 cut(s) 499
ApoI RAATTY 3 cut(s) 1152, 1235, 1258
ArsI GACNNNNNNTTYG 2 cut(s) 792, 824
AsiGI ACCGGT 1 cut(s) 1273
AspLEI GCGC 3 cut(s) 500, 733, 764
AspS9I GGNCC 8 cut(s) 156, 597, 748, 808, 1101, 1131, 1594, 1619
AsuC2I CCSGG 9 cut(s) 234, 235, 264, 265, 1129, 1325, 1472, 1632, 1794
AsuHPI GGTGA 2 cut(s) 1354, 1782
AsuII TTCGAA 1 cut(s) 401
AsuNHI GCTAGC 1 cut(s) 938
AvaI CYCGRG 4 cut(s) 233, 263, 484, 1551
AvaII GGWCC 3 cut(s) 597, 808, 1131
BalI TGGCCA 1 cut(s) 1415
BanI GGYRCC 1 cut(s) 730
BanII GRGCYC 2 cut(s) 242, 264
BbsI GAAGAC 1 cut(s) 957
Bbv12I GWGCWC 3 cut(s) 242, 509, 837
BccI CCATC 4 cut(s) 355, 610, 1037, 1675
BceAI ACGGC 5 cut(s) 144, 499, 629, 677, 1032
BciT130I CCWGG 5 cut(s) 709, 1025, 1337, 1445, 1733
BcnI CCSGG 9 cut(s) 234, 235, 264, 265, 1129, 1325, 1472, 1632, 1794
BcoDI GTCTC 6 cut(s) 458, 898, 1185, 1402, 1490, 1548
BfaI CTAG 1 cut(s) 939
BfmI CTRYAG 2 cut(s) 1571, 1756
BfoI RGCGCY 2 cut(s) 501, 734
BfuAI ACCTGC 2 cut(s) 414, 1564
BglI GCCNNNNNGGC 1 cut(s) 1567
BlpI GCTNAGC 1 cut(s) 45
BmcAI AGTACT 1 cut(s) 1511
Bme18I GGWCC 3 cut(s) 597, 808, 1131
BmeT110I CYCGRG 4 cut(s) 233, 263, 484, 1551
BmgT120I GGNCC 8 cut(s) 156, 597, 748, 808, 1101, 1131, 1594, 1619
BmiI GGNNCC 4 cut(s) 261, 599, 732, 1629
BmtI GCTAGC 1 cut(s) 942
BpiI GAAGAC 1 cut(s) 957
BplI GAGNNNNNCTC 4 cut(s) 332, 364, 902, 934
BpmI CTGGAG 3 cut(s) 691, 1271, 1340
Bpu1102I GCTNAGC 1 cut(s) 45
Bpu14I TTCGAA 1 cut(s) 401
BpuMI CCSGG 9 cut(s) 234, 235, 264, 265, 1129, 1325, 1472, 1632, 1794
BsaBI GATNNNNATC 1 cut(s) 291
BsaHI GRCGYC 4 cut(s) 654, 690, 731, 1202
BsaI GGTCTC 2 cut(s) 458, 1490
BsaJI CCNNGG 8 cut(s) 152, 233, 263, 441, 1087, 1443, 1444, 1793
BsaWI WCCGGW 6 cut(s) 784, 907, 1136, 1163, 1273, 1388
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 589, 619
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 283, 306, 871, 1184
Bse118I RCCGGY 2 cut(s) 1273, 1488
Bse1I ACTGG 4 cut(s) 1222, 1357, 1416, 1703
Bse3DI GCAATG 1 cut(s) 1527
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 5 cut(s) 709, 1025, 1337, 1445, 1733
BseDI CCNNGG 8 cut(s) 152, 233, 263, 441, 1087, 1443, 1444, 1793
BseGI GGATG 4 cut(s) 292, 366, 1029, 1333
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 5 cut(s) 185, 283, 306, 871, 1184
BseMI GCAATG 1 cut(s) 1527
BseMII CTCAG 4 cut(s) 59, 508, 829, 1397
BseNI ACTGG 4 cut(s) 1222, 1357, 1416, 1703
BseRI GAGGAG 3 cut(s) 133, 666, 1361
BseX3I CGGCCG 1 cut(s) 336
BseYI CCCAGC 1 cut(s) 1591
Bsh1236I CGCG 3 cut(s) 194, 425, 443
Bsh1285I CGRYCG 2 cut(s) 255, 339
BshNI GGYRCC 1 cut(s) 730
BshTI ACCGGT 1 cut(s) 1273
BsiEI CGRYCG 2 cut(s) 255, 339
BsiHKAI GWGCWC 3 cut(s) 242, 509, 837
BsiHKCI CYCGRG 4 cut(s) 233, 263, 484, 1551
BsiWI CGTACG 1 cut(s) 930
BslFI GGGAC 1 cut(s) 1808
BslI CCNNNNNNNGG 5 cut(s) 185, 283, 306, 871, 1184
BsmAI GTCTC 6 cut(s) 458, 898, 1185, 1402, 1490, 1548
BsmBI CGTCTC 1 cut(s) 1185
BsmFI GGGAC 1 cut(s) 1808
Bso31I GGTCTC 2 cut(s) 458, 1490
BsoBI CYCGRG 4 cut(s) 233, 263, 484, 1551
Bsp119I TTCGAA 1 cut(s) 401
Bsp1286I GDGCHC 4 cut(s) 242, 264, 509, 837
Bsp1407I TGTACA 2 cut(s) 14, 882
Bsp143I GATC 7 cut(s) 286, 466, 520, 720, 795, 841, 1158
Bsp1720I GCTNAGC 1 cut(s) 45
Bsp19I CCATGG 1 cut(s) 152
BspCNI CTCAG 4 cut(s) 58, 507, 828, 1398
BspFNI CGCG 3 cut(s) 194, 425, 443
BspHI TCATGA 1 cut(s) 1482
BspLI GGNNCC 4 cut(s) 261, 599, 732, 1629
BspMAI CTGCAG 1 cut(s) 1575
BspMI ACCTGC 2 cut(s) 414, 1564
BspOI GCTAGC 1 cut(s) 942
BspPI GGATC 4 cut(s) 294, 461, 836, 1166
BspQI GCTCTTC 1 cut(s) 514
BspT104I TTCGAA 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 730
BspTNI GGTCTC 2 cut(s) 458, 1490
BsrBI CCGCTC 3 cut(s) 335, 1106, 1175
BsrDI GCAATG 1 cut(s) 1527
BsrFI RCCGGY 2 cut(s) 1273, 1488
BsrGI TGTACA 2 cut(s) 14, 882
BsrI ACTGG 4 cut(s) 1222, 1357, 1416, 1703
BssAI RCCGGY 2 cut(s) 1273, 1488
BssECI CCNNGG 8 cut(s) 152, 233, 263, 441, 1087, 1443, 1444, 1793
BssMI GATC 7 cut(s) 286, 466, 520, 720, 795, 841, 1158
BssNAI GTATAC 1 cut(s) 1169
BssNI GRCGYC 4 cut(s) 654, 690, 731, 1202
BssT1I CCWWGG 2 cut(s) 152, 1087
Bst1107I GTATAC 1 cut(s) 1169
Bst2UI CCWGG 5 cut(s) 709, 1025, 1337, 1445, 1733
Bst4CI ACNGT 4 cut(s) 253, 368, 1230, 1837
Bst6I CTCTTC 1 cut(s) 514
BstACI GRCGYC 4 cut(s) 654, 690, 731, 1202
BstAUI TGTACA 2 cut(s) 14, 882
BstBI TTCGAA 1 cut(s) 401
BstC8I GCNNGC 6 cut(s) 591, 792, 940, 988, 1116, 1609
BstDEI CTNAG 5 cut(s) 45, 494, 815, 1406, 1712
BstDSI CCRYGG 2 cut(s) 152, 441
BstF5I GGATG 4 cut(s) 292, 366, 1029, 1333
BstFNI CGCG 3 cut(s) 194, 425, 443
BstH2I RGCGCY 2 cut(s) 501, 734
BstHHI GCGC 3 cut(s) 500, 733, 764
BstKTI GATC 7 cut(s) 289, 469, 523, 723, 798, 844, 1161
BstMAI GTCTC 6 cut(s) 458, 898, 1185, 1402, 1490, 1548
BstMBI GATC 7 cut(s) 286, 466, 520, 720, 795, 841, 1158
BstMCI CGRYCG 2 cut(s) 255, 339
BstNI CCWGG 5 cut(s) 709, 1025, 1337, 1445, 1733
BstNSI RCATGY 1 cut(s) 13
BstSFI CTRYAG 2 cut(s) 1571, 1756
BstUI CGCG 3 cut(s) 194, 425, 443
BstV2I GAAGAC 1 cut(s) 957
BstX2I RGATCY 2 cut(s) 841, 1158
BstYI RGATCY 2 cut(s) 841, 1158
BstZ17I GTATAC 1 cut(s) 1169
BstZI CGGCCG 1 cut(s) 336
BtgI CCRYGG 2 cut(s) 152, 441
BtsCI GGATG 4 cut(s) 292, 366, 1029, 1333
BtsI GCAGTG 2 cut(s) 310, 1673
BtsIMutI CAGTG 2 cut(s) 310, 1673
BveI ACCTGC 2 cut(s) 414, 1564
Cac8I GCNNGC 6 cut(s) 591, 792, 940, 988, 1116, 1609
CciI TCATGA 1 cut(s) 1482
CfoI GCGC 3 cut(s) 500, 733, 764
Cfr10I RCCGGY 2 cut(s) 1273, 1488
Cfr13I GGNCC 8 cut(s) 156, 597, 748, 808, 1101, 1131, 1594, 1619
Cfr42I CCGCGG 1 cut(s) 444
Cfr9I CCCGGG 2 cut(s) 233, 263
CseI GACGC 7 cut(s) 431, 662, 698, 884, 944, 992, 1191
Csp6I GTAC 6 cut(s) 15, 190, 883, 931, 1453, 1510
CspAI ACCGGT 1 cut(s) 1273
CspCI CAANNNNNGTGG 2 cut(s) 1375, 1410
CviAII CATG 6 cut(s) 10, 153, 283, 1483, 1546, 1845
CviQI GTAC 6 cut(s) 15, 190, 883, 931, 1453, 1510
DdeI CTNAG 5 cut(s) 45, 494, 815, 1406, 1712
DinI GGCGCC 1 cut(s) 732
DpnI GATC 7 cut(s) 288, 468, 522, 722, 797, 843, 1160
DpnII GATC 7 cut(s) 286, 466, 520, 720, 795, 841, 1158
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 2 cut(s) 336, 1413
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 514
EciI GGCGGA 4 cut(s) 345, 603, 609, 888
Ecl136II GAGCTC 1 cut(s) 240
EclXI CGGCCG 1 cut(s) 336
Eco130I CCWWGG 2 cut(s) 152, 1087
Eco147I AGGCCT 1 cut(s) 974
Eco24I GRGCYC 2 cut(s) 242, 264
Eco31I GGTCTC 2 cut(s) 458, 1490
Eco47I GGWCC 3 cut(s) 597, 808, 1131
Eco47III AGCGCT 1 cut(s) 499
Eco52I CGGCCG 1 cut(s) 336
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 764
Eco88I CYCGRG 4 cut(s) 233, 263, 484, 1551
EcoICRI GAGCTC 1 cut(s) 240
EcoO109I RGGNCCY 1 cut(s) 748
EcoRII CCWGG 5 cut(s) 707, 1023, 1335, 1443, 1731
EcoT14I CCWWGG 2 cut(s) 152, 1087
EcoT38I GRGCYC 2 cut(s) 242, 264
EgeI GGCGCC 1 cut(s) 732
EheI GGCGCC 1 cut(s) 732
ErhI CCWWGG 2 cut(s) 152, 1087
Esp3I CGTCTC 1 cut(s) 1185
FaeI CATG 6 cut(s) 13, 156, 286, 1486, 1549, 1848
FaqI GGGAC 1 cut(s) 1808
FatI CATG 6 cut(s) 9, 152, 282, 1482, 1545, 1844
FauI CCCGC 4 cut(s) 17, 931, 1180, 1604
FblI GTMKAC 4 cut(s) 490, 1071, 1168, 1320
FokI GGATG 4 cut(s) 279, 373, 1016, 1340
FriOI GRGCYC 2 cut(s) 242, 264
FspBI CTAG 1 cut(s) 939
GlaI GCGC 3 cut(s) 499, 732, 763
GsaI CCCAGC 1 cut(s) 1595
GsuI CTGGAG 3 cut(s) 691, 1271, 1340
HaeII RGCGCY 2 cut(s) 501, 734
HgaI GACGC 7 cut(s) 431, 662, 698, 884, 944, 992, 1191
HhaI GCGC 3 cut(s) 500, 733, 764
Hin1I GRCGYC 4 cut(s) 654, 690, 731, 1202
Hin1II CATG 6 cut(s) 13, 156, 286, 1486, 1549, 1848
Hin6I GCGC 3 cut(s) 498, 731, 762
HinP1I GCGC 3 cut(s) 498, 731, 762
HincII GTYRAC 3 cut(s) 491, 1072, 1321
HindII GTYRAC 3 cut(s) 491, 1072, 1321
HindIII AAGCTT 1 cut(s) 1244
HphI GGTGA 2 cut(s) 1354, 1782
Hpy166II GTNNAC 9 cut(s) 15, 491, 550, 597, 1072, 1134, 1169, 1321, 1345
Hpy188III TCNNGA 7 cut(s) 277, 1078, 1250, 1292, 1483, 1495, 1750
Hpy8I GTNNAC 9 cut(s) 15, 491, 550, 597, 1072, 1134, 1169, 1321, 1345
HpyAV CCTTC 4 cut(s) 481, 642, 739, 1810
HpyCH4III ACNGT 4 cut(s) 253, 368, 1230, 1837
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 4 cut(s) 1114, 1241, 1363, 1573
HpyF3I CTNAG 5 cut(s) 45, 494, 815, 1406, 1712
HpySE526I ACGT 1 cut(s) 546
Hsp92I GRCGYC 4 cut(s) 654, 690, 731, 1202
Hsp92II CATG 6 cut(s) 13, 156, 286, 1486, 1549, 1848
HspAI GCGC 3 cut(s) 498, 731, 762
KasI GGCGCC 1 cut(s) 730
KspI CCGCGG 1 cut(s) 444
Kzo9I GATC 7 cut(s) 286, 466, 520, 720, 795, 841, 1158
LguI GCTCTTC 1 cut(s) 514
MaeI CTAG 1 cut(s) 939
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 3 cut(s) 1438, 1530, 1705
MalI GATC 7 cut(s) 288, 468, 522, 722, 797, 843, 1160
MbiI CCGCTC 3 cut(s) 335, 1106, 1175
MboI GATC 7 cut(s) 286, 466, 520, 720, 795, 841, 1158
MboII GAAGA 8 cut(s) 501, 959, 962, 1069, 1444, 1736, 1779, 1833
MfeI CAATTG 2 cut(s) 581, 639
MflI RGATCY 2 cut(s) 841, 1158
MhlI GDGCHC 4 cut(s) 242, 264, 509, 837
MlsI TGGCCA 1 cut(s) 1415
MluCI AATT 7 cut(s) 581, 639, 1152, 1235, 1258, 1463, 1602
MluI ACGCGT 2 cut(s) 192, 423
MluNI TGGCCA 1 cut(s) 1415
Mly113I GGCGCC 1 cut(s) 731
MmeI TCCRAC 6 cut(s) 240, 267, 320, 598, 676, 869
Mox20I TGGCCA 1 cut(s) 1415
MscI TGGCCA 1 cut(s) 1415
MseI TTAA 4 cut(s) 648, 1299, 1349, 1852
MslI CAYNNNNRTG 1 cut(s) 1520
Msp20I TGGCCA 1 cut(s) 1415
MspA1I CMGCKG 3 cut(s) 24, 229, 443
MunI CAATTG 2 cut(s) 581, 639
MvaI CCWGG 5 cut(s) 709, 1025, 1337, 1445, 1733
MvnI CGCG 3 cut(s) 194, 425, 443
NarI GGCGCC 1 cut(s) 731
NciI CCSGG 9 cut(s) 234, 235, 264, 265, 1129, 1325, 1472, 1632, 1794
NcoI CCATGG 1 cut(s) 152
NdeII GATC 7 cut(s) 286, 466, 520, 720, 795, 841, 1158
NheI GCTAGC 1 cut(s) 938
NlaIII CATG 6 cut(s) 13, 156, 286, 1486, 1549, 1848
NlaIV GGNNCC 4 cut(s) 261, 599, 732, 1629
NmeAIII GCCGAG 2 cut(s) 364, 914
NmuCI GTSAC 2 cut(s) 1438, 1530
NspI RCATGY 1 cut(s) 13
NspV TTCGAA 1 cut(s) 401
PagI TCATGA 1 cut(s) 1482
PaqCI CACCTGC 1 cut(s) 1564
PasI CCCWGGG 1 cut(s) 1444
PceI AGGCCT 1 cut(s) 974
PciI ACATGT 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 514
PcsI WCGNNNNNNNCGW 5 cut(s) 351, 398, 942, 1049, 1427
PfeI GAWTC 8 cut(s) 28, 386, 725, 1060, 1288, 1379, 1741, 1784
Pfl23II CGTACG 1 cut(s) 930
PflFI GACNNNGTC 1 cut(s) 692
PinAI ACCGGT 1 cut(s) 1273
PluTI GGCGCC 1 cut(s) 734
PscI ACATGT 1 cut(s) 9
PsiI TTATAA 1 cut(s) 969
Psp124BI GAGCTC 1 cut(s) 242
Psp6I CCWGG 5 cut(s) 707, 1023, 1335, 1443, 1731
PspFI CCCAGC 1 cut(s) 1591
PspGI CCWGG 5 cut(s) 707, 1023, 1335, 1443, 1731
PspLI CGTACG 1 cut(s) 930
PspN4I GGNNCC 4 cut(s) 261, 599, 732, 1629
PspPI GGNCC 8 cut(s) 156, 597, 748, 808, 1101, 1131, 1594, 1619
PstI CTGCAG 1 cut(s) 1575
PsuI RGATCY 2 cut(s) 841, 1158
PsyI GACNNNGTC 1 cut(s) 692
PvuII CAGCTG 1 cut(s) 229
RsaI GTAC 6 cut(s) 16, 191, 884, 932, 1454, 1511
RsaNI GTAC 6 cut(s) 15, 190, 883, 931, 1453, 1510
RseI CAYNNNNRTG 1 cut(s) 1520
SacI GAGCTC 1 cut(s) 242
SacII CCGCGG 1 cut(s) 444
SalI GTCGAC 3 cut(s) 489, 1070, 1319
SapI GCTCTTC 1 cut(s) 514
SaqAI TTAA 4 cut(s) 648, 1299, 1349, 1852
Sau3AI GATC 7 cut(s) 286, 466, 520, 720, 795, 841, 1158
Sau96I GGNCC 8 cut(s) 156, 597, 748, 808, 1101, 1131, 1594, 1619
ScaI AGTACT 1 cut(s) 1511
SduI GDGCHC 4 cut(s) 242, 264, 509, 837
SfcI CTRYAG 2 cut(s) 1571, 1756
SfoI GGCGCC 1 cut(s) 732
Sfr303I CCGCGG 1 cut(s) 444
SfuI TTCGAA 1 cut(s) 401
SgrBI CCGCGG 1 cut(s) 444
SinI GGWCC 3 cut(s) 597, 808, 1131
SmaI CCCGGG 2 cut(s) 235, 265
SmiMI CAYNNNNRTG 1 cut(s) 1520
Sse9I AATT 7 cut(s) 581, 639, 1152, 1235, 1258, 1463, 1602
SseBI AGGCCT 1 cut(s) 974
SspDI GGCGCC 1 cut(s) 730
SspMI CTAG 1 cut(s) 939
SstI GAGCTC 1 cut(s) 242
StuI AGGCCT 1 cut(s) 974
StyI CCWWGG 2 cut(s) 152, 1087
TaaI ACNGT 4 cut(s) 253, 368, 1230, 1837
TaiI ACGT 1 cut(s) 549
TaqII GACCGA 1 cut(s) 440
TasI AATT 7 cut(s) 581, 639, 1152, 1235, 1258, 1463, 1602
TatI WGTACW 3 cut(s) 14, 882, 1509
TfiI GAWTC 8 cut(s) 28, 386, 725, 1060, 1288, 1379, 1741, 1784
Tru1I TTAA 4 cut(s) 648, 1299, 1349, 1852
Tru9I TTAA 4 cut(s) 648, 1299, 1349, 1852
TscAI CASTG 2 cut(s) 310, 1673
TseFI GTSAC 2 cut(s) 1438, 1530
Tsp45I GTSAC 2 cut(s) 1438, 1530
TspDTI ATGAA 2 cut(s) 1471, 1833
TspGWI ACGGA 8 cut(s) 261, 407, 492, 814, 852, 887, 1058, 1167
TspMI CCCGGG 2 cut(s) 233, 263
TspRI CASTG 2 cut(s) 310, 1673
Tth111I GACNNNGTC 1 cut(s) 692
VpaK11BI GGWCC 3 cut(s) 597, 808, 1131
XapI RAATTY 3 cut(s) 1152, 1235, 1258
XceI RCATGY 1 cut(s) 13
XmaI CCCGGG 2 cut(s) 233, 263
XmiI GTMKAC 4 cut(s) 490, 1071, 1168, 1320
XspI CTAG 1 cut(s) 939
ZrmI AGTACT 1 cut(s) 1511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.