FvH4_5g32060

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006129
Physical Location & Seq
Reverse (-)
23139338 .. 23149666
10329 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32060.t4

Sequence Viewer

Length: 2844 bp
ATGGAAAATCGAAAACTATATATTGTTACTGTTGACATATTCTGTTTCCGCGGACTTCTACTTCCCAGTACCAACAGTCGGGAAAAAAAAGGCTTCATTTCAATGTCGAAGCAATCCGAGAATTCGAAGCCCCCGTACGTCCGGTCATCCGCCGCCAAAGTCCACCCCTCCGTCGACCTCGACGCCGACCCCAGCTCCTACTCCCTCGAAAAATTCCGCCTCTACGAAACCAGACAGCGGTTTTATCTGATCGGCAGCGATCGGAACAAGAGATTCTACAGAGTGCTGAAGATTGACCGCTCGGAACCGGATGATTTGAATTTAAGCGAAGATCCGGTTGTGTATTCTCCGCAAGAGATCAAGAATCTGCTCCAGCGAATCGCCGAGGGAAATCGAGCCACCGGAGGGCTCACTTTCGTCGCCAAGGTGTTTGGGATCGCCGGTTGCATTAAGTTCATGGAATCGTATTACTTGATTTTGGTTACGAAGCGTCGGCAGATTGGGAGTATATGTGGTCATGCAATTTATAGTATAGATGAGAGCCAGCTGATAACTATTCCGCATGTCACGATTCAAACTGATATTGCACATTCTAAGACGGAGCTACGGTACAAGAAGCTTTTGTCGAGTGTCGATTTGACCAAAGATTTTTTCTATAGCTATACATATCCAATAATGCAAAGTCTGCAAAAAAATGTGGTATCAATGGGTGAAGAAGGGATGCAGTATGAAAATATATTTGTATGGAATGCCTATTTAACACAAGCAATTCGCTCAAGGTGTAATAACACAATCTGGACAATAGCATTAGTGCATGGGCATTTTAAGCAGGCTAGGCTATCAATCTTTGGGAGGGACTTCAGTGTTTCTCTGGTGTCTAGACGCTCTCGGCATTTTGCCGGAACACGTTACTTGAAAAGGGGGGTGAATGATAGGGGAAGGGTTGCAAATGATGTTGAGACAGAGCAGATTATCCTTGATGAAGAAGCTGGTTCATGCAAGGGAAAAATGAGTTCTGTTGTCCAGATGCGTGGGTCAATTCCCCTCTTTTGGTCACAAGAAGCTTCAAGATTTAGTCCTAAACCTGATATAATCTTACAGAGATATGATCCTACATATCAGGCTACCAAATTTCATTTTGAAGACCTGGCAAGGAGATATGGAAACCCGATTATTGTTCTTAATTTAATCAAGACTGTTGAAAAGAGGCCCCGAGAAATGATGCTGAGGCGTGAGTTCGCAAATGCGGTTGGGTATCTGAACCAAATTTTTCCGGAAGAAAATCATCTAAAATTTATCCACTGGGACTTTCACAAGTTTGCAAAAAGCAAGTCTGCCAACGTTTTAGCTGTTCTGGGTGCTGTGGCAAGTGAAGCACTCGATTTGACTGGATTTTACTACAGTGGAAAACCTAGCGTTGTTAAACGGAGAATCAATCAGATGAGTCGAACAAGTACTGGAAGGGAAGCATCTCTTAGAGGTCTGAGAGCCAGTTCTGGGGACCTTCCCAGGTTTGGGAGCAGTAATGAAACTCTTAATCCTTCTGTTAGTCGAGATAGAGATGCTGATATTGGCCAACAGAAGAAAAGTGATAATTCTGGTGGCGAACCACCACACTTTCAAAGTGGGGTTTTGCGCACAAACTGCATTGATTGCTTGGATCGTACAAATGTTGCCCAGTATGCTTATGGCCTTGCAGCTTTAGGCCGCCAACTTCATGCAATGGGGTTGACTAACATACCAAAAGTTGATCCTGATAGCAGTATTGCTGCAGCCCTCATGGATATGTACCAGAGCATGGGGGATGCTCTTGCCCAGCAGTATGGTGGTTCTGCAGCTCACAACACTGTATTTCCTGAGAGGCAGGGAAAGTGGAAAGCTACAACTCAATCAAGAGAGTTTCTAAAGTCTATCAAGCGATACTACAGCAATGCTTACACTGATGGTGAAAAGCAAGATGCAATAAATTTATTTTTGGGTTACTTCAAGCCACAGGAAGGGAAACCTGCTCTGTGGGAGCTGGACTCTGATTACTATCTTCATGTATCTGGGCTTGATGATCTTCTCCTTGATTTGTGCTCACAAGCCAATGAAATGCACTCAGGAGCATTGGGAAACACTCTTGCCCCTATTCCAGCTTGCAAAGAAGACTTTTTGAGGATGAAGCTGACATCATTTGACAAATTGATTGAAAGAACATGCAGTTCAATAAAGAATGTAAGACTTTGCAGCGAACCAGACCAGAGACCAGGTGGTGCTGCTGCAAATTCTAGTGTGGCACCTGACGCAGCTGAAATACAGTTGAAGAGCCCTAATTGGCTTTTTGGCCAGAGAAAGTATGAAGAAAGTGGATCTGCACCAAAAGTTACTTCACATGTAGTCATAAATGGAGGATCCCATAATGACCCAGAAGTTGATGGCTATTGTGACCTAAATTGGCTTTCTTCTGGTGGCAATGATAACGAGGAGGATATATTCCAGAGGTACCTTTCAATGACATCAGTTGATGAAGCCAATGGGTGGTACGGTGGGACTCTGTTAGGTGATCAAAATGAAAGCAGTGAGATATATAAGCATTATGCTGAGTTATGTCAGGGCCCTGCCATTGAACCGTTCCAAAATGATTTGGAGAGAGAGAAGCACTATGCTGATGCTCTTCACATGGGAACAATAAATATTCTGGATGATGCTGCTGTTGAGGCAGAGATGGAAGCAGCTCTCAAGGAGTATGATCAAATTGGAAGTGATCTTGTGAGCATCCCTACAGCTTGTAAATCATTTGCTGATGATCCGAGTTGGTTGACAAGATGGATCATTGGGGAAGAGAAGGTGCAAAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000902 GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005794 GO:0005811 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006996 GO:0007010 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0008610 GO:0008654 GO:0009058 GO:0009653 GO:0009826 GO:0009832 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031901 GO:0031902 GO:0031982 GO:0031984 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034595 GO:0034596 GO:0036092 GO:0040007 GO:0042546 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0043813 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046474 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048589 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0060284 GO:0060560 GO:0065007 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0090407 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0106018 GO:0120035 GO:1901576 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

948

Amino Acids

106.62

Weight (kDa)

6.75

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 144 - 440 2.3e-81 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1637
Acc36I ACCTGC 1 cut(s) 2014
Acc65I GGTACC 1 cut(s) 2484
AccB1I GGYRCC 2 cut(s) 2278, 2484
AccB7I CCANNNNNTGG 2 cut(s) 1798, 2520
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 51
AccIII TCCGGA 1 cut(s) 1273
AclI AACGTT 1 cut(s) 1341
AcoI YGGCCR 2 cut(s) 1573, 2326
AcsI RAATTY 8 cut(s) 121, 212, 319, 1130, 1266, 1292, 1966, 2266
AcuI CTGAAG 2 cut(s) 308, 844
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 8 cut(s) 70, 137, 611, 1456, 1666, 1790, 2486, 2525
AflIII ACRYGT 2 cut(s) 905, 2374
AjnI CCWGG 3 cut(s) 1146, 1508, 2248
AjuI GAANNNNNNNTTGG 4 cut(s) 635, 667, 2353, 2385
Alw21I GWGCWC 1 cut(s) 2081
Alw26I GTCTC 2 cut(s) 953, 2239
Ama87I CYCGRG 1 cut(s) 1212
Aor13HI TCCGGA 1 cut(s) 1273
AoxI GGCC 6 cut(s) 1208, 1573, 1690, 1705, 2326, 2596
ApaI GGGCCC 1 cut(s) 2600
ApoI RAATTY 8 cut(s) 121, 212, 319, 1130, 1266, 1292, 1966, 2266
ArsI GACNNNNNNTTYG 2 cut(s) 1316, 1348
Asp718I GGTACC 1 cut(s) 2484
AspLEI GCGC 1 cut(s) 1638
AspS9I GGNCC 4 cut(s) 1209, 1501, 2596, 2597
AsuHPI GGTGA 4 cut(s) 722, 937, 1958, 2555
AsuII TTCGAA 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 1212
AvaII GGWCC 1 cut(s) 1501
BaeGI GKGCMC 1 cut(s) 2600
BalI TGGCCA 2 cut(s) 1575, 2328
BamHI GGATCC 1 cut(s) 2393
BanI GGYRCC 2 cut(s) 2278, 2484
BanII GRGCYC 3 cut(s) 411, 2312, 2600
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BbsI GAAGAC 2 cut(s) 1149, 2154
Bbv12I GWGCWC 1 cut(s) 2081
BbvCI CCTCAGC 1 cut(s) 1226
BccI CCATC 4 cut(s) 1937, 2411, 2701, 2802
BciT130I CCWGG 3 cut(s) 1148, 1510, 2250
BclI TGATCA 2 cut(s) 2545, 2731
BcoDI GTCTC 2 cut(s) 953, 2239
BfaI CTAG 4 cut(s) 834, 879, 1413, 2271
BfmI CTRYAG 7 cut(s) 277, 655, 1399, 1770, 1833, 1924, 2763
BfuAI ACCTGC 1 cut(s) 2014
BmcAI AGTACT 1 cut(s) 1456
Bme1390I CCNGG 3 cut(s) 1148, 1510, 2250
Bme18I GGWCC 1 cut(s) 1501
BmeT110I CYCGRG 1 cut(s) 1212
BmgT120I GGNCC 4 cut(s) 1209, 1501, 2596, 2597
BmiI GGNNCC 7 cut(s) 306, 1211, 1502, 2280, 2395, 2486, 2598
BmrFI CCNGG 3 cut(s) 1148, 1510, 2250
BmrI ACTGGG 3 cut(s) 60, 1312, 1672
BmuI ACTGGG 3 cut(s) 60, 1312, 1672
BpiI GAAGAC 2 cut(s) 1149, 2154
BplI GAGNNNNNCTC 2 cut(s) 2009, 2041
BpmI CTGGAG 1 cut(s) 356
Bpu10I CCTNAGC 1 cut(s) 1226
Bpu14I TTCGAA 1 cut(s) 125
BpuEI CTTGAG 2 cut(s) 760, 2705
BsaBI GATNNNNATC 1 cut(s) 2034
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 2239
BsaJI CCNNGG 4 cut(s) 49, 384, 423, 1508
BsaWI WCCGGW 5 cut(s) 141, 307, 334, 401, 1273
BsaXI ACNNNNNCTCC 6 cut(s) 179, 209, 496, 526, 593, 623
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 6 cut(s) 66, 1307, 1393, 1462, 1491, 1678
Bse3DI GCAATG 3 cut(s) 1728, 1936, 2461
Bse8I GATNNNNATC 1 cut(s) 2034
BseAI TCCGGA 1 cut(s) 1273
BseBI CCWGG 3 cut(s) 1148, 1510, 2250
BseDI CCNNGG 4 cut(s) 49, 384, 423, 1508
BseGI GGATG 7 cut(s) 146, 316, 726, 1810, 2166, 2689, 2757
BseJI GATNNNNATC 1 cut(s) 2034
BseMI GCAATG 3 cut(s) 1728, 1936, 2461
BseMII CTCAG 5 cut(s) 1217, 1475, 1848, 2115, 2574
BseNI ACTGG 6 cut(s) 66, 1307, 1393, 1462, 1491, 1678
BseRI GAGGAG 1 cut(s) 2480
BseSI GKGCMC 1 cut(s) 2600
BseYI CCCAGC 2 cut(s) 191, 1815
BsgI GTGCAG 1 cut(s) 2340
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 6 cut(s) 1210, 1575, 1692, 1707, 2328, 2598
BshNI GGYRCC 2 cut(s) 2278, 2484
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 2081
BsiHKCI CYCGRG 1 cut(s) 1212
BsiSI CCGG 7 cut(s) 142, 308, 335, 402, 441, 900, 1274
BsiWI CGTACG 1 cut(s) 135
BslFI GGGAC 4 cut(s) 869, 1319, 1514, 2545
BsmAI GTCTC 2 cut(s) 953, 2239
BsmFI GGGAC 4 cut(s) 869, 1319, 1514, 2545
BsmI GAATGC 1 cut(s) 754
BsnI GGCC 6 cut(s) 1210, 1575, 1692, 1707, 2328, 2598
Bso31I GGTCTC 1 cut(s) 2239
BsoBI CYCGRG 1 cut(s) 1212
Bsp119I TTCGAA 1 cut(s) 125
Bsp120I GGGCCC 1 cut(s) 2596
Bsp1286I GDGCHC 4 cut(s) 411, 2081, 2312, 2600
Bsp13I TCCGGA 1 cut(s) 1273
BspANI GGCC 6 cut(s) 1210, 1575, 1692, 1707, 2328, 2598
BspCNI CTCAG 5 cut(s) 1218, 1476, 1849, 2114, 2575
BspEI TCCGGA 1 cut(s) 1273
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 7 cut(s) 306, 1211, 1502, 2280, 2395, 2486, 2598
BspMAI CTGCAG 2 cut(s) 1774, 1837
BspMI ACCTGC 1 cut(s) 2014
BspQI GCTCTTC 2 cut(s) 2300, 2661
BspT104I TTCGAA 1 cut(s) 125
BspT107I GGYRCC 2 cut(s) 2278, 2484
BspTNI GGTCTC 1 cut(s) 2239
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 3 cut(s) 1728, 1936, 2461
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 6 cut(s) 66, 1307, 1393, 1462, 1491, 1678
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 4 cut(s) 49, 384, 423, 1508
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 3 cut(s) 1148, 1510, 2250
Bst4CI ACNGT 9 cut(s) 31, 77, 609, 1198, 1403, 1849, 2301, 2528, 2613
Bst6I CTCTTC 3 cut(s) 2300, 2661, 2817
BstACI GRCGYC 1 cut(s) 183
BstAPI GCANNNNNTGC 3 cut(s) 685, 1644, 1653
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 3 cut(s) 545, 831, 2140
BstDEI CTNAG 7 cut(s) 594, 1226, 1475, 1484, 1857, 2101, 2583
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 7 cut(s) 146, 316, 726, 1810, 2166, 2689, 2757
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 1 cut(s) 1638
BstMAI GTCTC 2 cut(s) 953, 2239
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 9 cut(s) 685, 826, 835, 1373, 1644, 1653, 1682, 2285, 2699
BstNI CCWGG 3 cut(s) 1148, 1510, 2250
BstNSI RCATGY 3 cut(s) 566, 2202, 2378
BstSCI CCNGG 3 cut(s) 1146, 1508, 2248
BstSFI CTRYAG 7 cut(s) 277, 655, 1399, 1770, 1833, 1924, 2763
BstSLI GKGCMC 1 cut(s) 2600
BstUI CGCG 1 cut(s) 51
BstV2I GAAGAC 2 cut(s) 1149, 2154
BstX2I RGATCY 3 cut(s) 331, 2351, 2393
BstXI CCANNNNNNTGG 2 cut(s) 1031, 1823
BstYI RGATCY 3 cut(s) 331, 2351, 2393
BsuRI GGCC 6 cut(s) 1210, 1575, 1692, 1707, 2328, 2598
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 7 cut(s) 146, 316, 726, 1810, 2166, 2689, 2757
BtsI GCAGTG 1 cut(s) 2566
BtsIMutI CAGTG 6 cut(s) 868, 1300, 1408, 1845, 1938, 2566
BveI ACCTGC 1 cut(s) 2014
Cac8I GCNNGC 3 cut(s) 545, 831, 2140
CfoI GCGC 1 cut(s) 1638
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 4 cut(s) 1209, 1501, 2596, 2597
Cfr42I CCGCGG 1 cut(s) 52
CseI GACGC 4 cut(s) 191, 479, 891, 2294
CsiI ACCWGGT 1 cut(s) 2248
Csp6I GTAC 8 cut(s) 69, 136, 610, 1455, 1665, 1789, 2485, 2524
CviQI GTAC 8 cut(s) 69, 136, 610, 1455, 1665, 1789, 2485, 2524
DdeI CTNAG 7 cut(s) 594, 1226, 1475, 1484, 1857, 2101, 2583
EaeI YGGCCR 2 cut(s) 1573, 2326
Eam1104I CTCTTC 3 cut(s) 2300, 2661, 2817
EarI CTCTTC 3 cut(s) 2300, 2661, 2817
EciI GGCGGA 2 cut(s) 139, 206
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 3 cut(s) 411, 2312, 2600
Eco31I GGTCTC 1 cut(s) 2239
Eco47I GGWCC 1 cut(s) 1501
Eco57I CTGAAG 2 cut(s) 308, 844
Eco88I CYCGRG 1 cut(s) 1212
EcoO109I RGGNCCY 4 cut(s) 1209, 1501, 2596, 2597
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 1146, 1508, 2248
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 3 cut(s) 411, 2312, 2600
ErhI CCWWGG 1 cut(s) 423
FalI AAGNNNNNCTT 2 cut(s) 1458, 1490
FaqI GGGAC 4 cut(s) 869, 1319, 1514, 2545
FbaI TGATCA 2 cut(s) 2545, 2731
FblI GTMKAC 1 cut(s) 174
FokI GGATG 7 cut(s) 133, 323, 733, 1817, 2173, 2696, 2744
FriOI GRGCYC 3 cut(s) 411, 2312, 2600
FspBI CTAG 4 cut(s) 834, 879, 1413, 2271
FspI TGCGCA 1 cut(s) 1637
GlaI GCGC 1 cut(s) 1637
GsaI CCCAGC 2 cut(s) 195, 1819
GsuI CTGGAG 1 cut(s) 356
HaeIII GGCC 6 cut(s) 1210, 1575, 1692, 1707, 2328, 2598
HapII CCGG 7 cut(s) 142, 308, 335, 402, 441, 900, 1274
HgaI GACGC 4 cut(s) 191, 479, 891, 2294
HhaI GCGC 1 cut(s) 1638
Hin1I GRCGYC 1 cut(s) 183
Hin6I GCGC 1 cut(s) 1636
HinP1I GCGC 1 cut(s) 1636
HincII GTYRAC 4 cut(s) 34, 175, 1731, 2802
HindII GTYRAC 4 cut(s) 34, 175, 1731, 2802
HindIII AAGCTT 2 cut(s) 617, 1062
HinfI GANTC 9 cut(s) 273, 364, 378, 461, 571, 1431, 1444, 2024, 2533
HpaII CCGG 7 cut(s) 142, 308, 335, 402, 441, 900, 1274
HphI GGTGA 4 cut(s) 722, 937, 1958, 2555
Hpy166II GTNNAC 5 cut(s) 34, 163, 175, 1731, 2802
Hpy188I TCNGA 9 cut(s) 118, 249, 264, 304, 1260, 1440, 1485, 2029, 2793
Hpy8I GTNNAC 5 cut(s) 34, 163, 175, 1731, 2802
Hpy99I CGWCG 4 cut(s) 176, 185, 422, 495
HpyAV CCTTC 7 cut(s) 710, 933, 1455, 1514, 1551, 1991, 2821
HpyCH4III ACNGT 9 cut(s) 31, 77, 609, 1198, 1403, 1849, 2301, 2528, 2613
HpyCH4IV ACGT 3 cut(s) 138, 907, 1341
HpyF10VI GCNNNNNNNGC 9 cut(s) 685, 826, 835, 1373, 1644, 1653, 1682, 2285, 2699
HpyF3I CTNAG 7 cut(s) 594, 1226, 1475, 1484, 1857, 2101, 2583
HpySE526I ACGT 3 cut(s) 138, 907, 1341
Hsp92I GRCGYC 1 cut(s) 183
HspAI GCGC 1 cut(s) 1636
Kpn2I TCCGGA 1 cut(s) 1273
KpnI GGTACC 1 cut(s) 2488
Ksp22I TGATCA 2 cut(s) 2545, 2731
KspI CCGCGG 1 cut(s) 52
LguI GCTCTTC 2 cut(s) 2300, 2661
LmnI GCTCC 6 cut(s) 200, 375, 601, 1518, 2017, 2105
MabI ACCWGGT 1 cut(s) 2248
MaeI CTAG 4 cut(s) 834, 879, 1413, 2271
MaeII ACGT 3 cut(s) 138, 907, 1341
MaeIII GTNAC 8 cut(s) 25, 481, 565, 908, 1053, 1979, 2365, 2426
MbiI CCGCTC 1 cut(s) 300
MflI RGATCY 3 cut(s) 331, 2351, 2393
MhlI GDGCHC 4 cut(s) 411, 2081, 2312, 2600
MlsI TGGCCA 2 cut(s) 1575, 2328
MluNI TGGCCA 2 cut(s) 1575, 2328
MlyI GAGTC 3 cut(s) 1453, 2018, 2527
Mox20I TGGCCA 2 cut(s) 1575, 2328
MroI TCCGGA 1 cut(s) 1273
MscI TGGCCA 2 cut(s) 1575, 2328
MseI TTAA 8 cut(s) 323, 450, 758, 825, 1182, 1187, 1422, 1536
MslI CAYNNNNRTG 2 cut(s) 101, 1784
Msp20I TGGCCA 2 cut(s) 1575, 2328
MspA1I CMGCKG 4 cut(s) 51, 238, 547, 2291
MspI CCGG 7 cut(s) 142, 308, 335, 402, 441, 900, 1274
MspR9I CCNGG 3 cut(s) 1148, 1510, 2250
Mva1269I GAATGC 1 cut(s) 754
MvaI CCWGG 3 cut(s) 1148, 1510, 2250
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 9 cut(s) 685, 826, 835, 1373, 1644, 1653, 1682, 2285, 2699
NlaIV GGNNCC 7 cut(s) 306, 1211, 1502, 2280, 2395, 2486, 2598
NmeAIII GCCGAG 2 cut(s) 409, 868
NmuCI GTSAC 3 cut(s) 565, 1053, 2426
NsbI TGCGCA 1 cut(s) 1637
NspI RCATGY 3 cut(s) 566, 2202, 2378
NspV TTCGAA 1 cut(s) 125
PciI ACATGT 1 cut(s) 2374
PciSI GCTCTTC 2 cut(s) 2300, 2661
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PctI GAATGC 1 cut(s) 754
PfeI GAWTC 6 cut(s) 273, 364, 378, 461, 571, 1431
Pfl23II CGTACG 1 cut(s) 135
PflMI CCANNNNNTGG 2 cut(s) 1798, 2520
Ple19I CGATCG 1 cut(s) 262
PleI GAGTC 3 cut(s) 1452, 2018, 2527
PpsI GAGTC 3 cut(s) 1452, 2018, 2527
PpuMI RGGWCCY 1 cut(s) 1501
PscI ACATGT 1 cut(s) 2374
Psp1406I AACGTT 1 cut(s) 1341
Psp5II RGGWCCY 1 cut(s) 1501
Psp6I CCWGG 3 cut(s) 1146, 1508, 2248
PspFI CCCAGC 2 cut(s) 191, 1815
PspGI CCWGG 3 cut(s) 1146, 1508, 2248
PspLI CGTACG 1 cut(s) 135
PspN4I GGNNCC 7 cut(s) 306, 1211, 1502, 2280, 2395, 2486, 2598
PspOMI GGGCCC 1 cut(s) 2596
PspPI GGNCC 4 cut(s) 1209, 1501, 2596, 2597
PspPPI RGGWCCY 1 cut(s) 1501
PstI CTGCAG 2 cut(s) 1774, 1837
PsuI RGATCY 3 cut(s) 331, 2351, 2393
PvuI CGATCG 1 cut(s) 262
PvuII CAGCTG 2 cut(s) 547, 2291
RsaI GTAC 8 cut(s) 70, 137, 611, 1456, 1666, 1790, 2486, 2525
RsaNI GTAC 8 cut(s) 69, 136, 610, 1455, 1665, 1789, 2485, 2524
RseI CAYNNNNRTG 2 cut(s) 101, 1784
SacII CCGCGG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 173
SapI GCTCTTC 2 cut(s) 2300, 2661
SaqAI TTAA 8 cut(s) 323, 450, 758, 825, 1182, 1187, 1422, 1536
Sau96I GGNCC 4 cut(s) 1209, 1501, 2596, 2597
ScaI AGTACT 1 cut(s) 1456
SchI GAGTC 3 cut(s) 1453, 2018, 2527
ScrFI CCNGG 3 cut(s) 1148, 1510, 2250
SduI GDGCHC 4 cut(s) 411, 2081, 2312, 2600
SexAI ACCWGGT 1 cut(s) 2248
SfcI CTRYAG 7 cut(s) 277, 655, 1399, 1770, 1833, 1924, 2763
Sfr303I CCGCGG 1 cut(s) 52
SfuI TTCGAA 1 cut(s) 125
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 1 cut(s) 1501
SmiMI CAYNNNNRTG 2 cut(s) 101, 1784
SmlI CTYRAG 2 cut(s) 775, 2720
SmoI CTYRAG 2 cut(s) 775, 2720
SspI AATATT 1 cut(s) 2677
SspMI CTAG 4 cut(s) 834, 879, 1413, 2271
StyD4I CCNGG 3 cut(s) 1146, 1508, 2248
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 9 cut(s) 31, 77, 609, 1198, 1403, 1849, 2301, 2528, 2613
TaiI ACGT 3 cut(s) 141, 910, 1344
TatI WGTACW 1 cut(s) 1454
TauI GCSGC 2 cut(s) 155, 1710
TfiI GAWTC 6 cut(s) 273, 364, 378, 461, 571, 1431
Tru1I TTAA 8 cut(s) 323, 450, 758, 825, 1182, 1187, 1422, 1536
Tru9I TTAA 8 cut(s) 323, 450, 758, 825, 1182, 1187, 1422, 1536
TscAI CASTG 6 cut(s) 868, 1307, 1408, 1852, 1945, 2566
TseFI GTSAC 3 cut(s) 565, 1053, 2426
Tsp45I GTSAC 3 cut(s) 565, 1053, 2426
TspGWI ACGGA 3 cut(s) 160, 614, 1441
TspRI CASTG 6 cut(s) 868, 1307, 1408, 1852, 1945, 2566
Van91I CCANNNNNTGG 2 cut(s) 1798, 2520
VpaK11BI GGWCC 1 cut(s) 1501
XapI RAATTY 8 cut(s) 121, 212, 319, 1130, 1266, 1292, 1966, 2266
XbaI TCTAGA 1 cut(s) 878
XceI RCATGY 3 cut(s) 566, 2202, 2378
XcmI CCANNNNNNNNNTGG 3 cut(s) 1685, 1823, 2249
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 4 cut(s) 834, 879, 1413, 2271
ZrmI AGTACT 1 cut(s) 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.