Rroxscaffold_3G00231490

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006129
Physical Location & Seq
Forward (+)
16760490 .. 16771070
10581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00231490.1

Sequence Viewer

Length: 2772 bp
ATGGCGAAACAAGCCGAGAATTCGAAGCGCCCGTACGTCCGGTCCTCCGCCGCAAAAGTCCACCCCTCCGTCGACCTCGACGCCGACCCCAACACCTACTCCCTCGAAAAATTCCGCCTCTACGAAACCAGACAGCGGTTTTATTTGATCGGGAGCGATCGGAACAAGAGATTCTACAGAGTGCTGAAGATAGACCGGTCGGAGCCGGATGATTTGAATATAAGCGAAGATCCGGTGGTTTACTCGCCGCAAGAGATCAAGAATCTGCTTCAGCGAATCGATGAGGGAAATCGAGCCACCGGAGGCCTTACTTTCGTCGCCAAGGTGTTTGGGATTGCCGGATGCATTAAGTTTATGGAATCGTATTACTTGATTTTGGTTACGAAGCGTCGGCAGATTGGAAGTATATGCGGTCATGCAATTTATGGTATAGATGAGAGTCAGCTGATAACTATTCCGCATGTCACGATTCAAACAGATATTGCACATTCTAAGACAGAGCTGCGGTACAAGAAGCTTTTGTCGAGTGTTGATTTGACCAAAGATTTTTTCTATAGCTATACATATCCTATAATGCAAAGTCTGCAAAAGAATGTGGTATCAATGGGTGAAGAAGGGATGCAGTATGAAAATATATTCGTATGGAATGCCTATCTAACACAAGCAATTCGCTCAAGGTGTAATAATACAATCTGGACAATAGCATTAGTACATGGGCATTTTAAGCAGACTAGGCTATCAATCTTTGGGAGGGACTTCAGTGTTTCTCTGGTGTCTAGGCGCTCTCGGCATTTTGCAGGGACACGGGTTTATATTTTAATGTTGATTGCATTGCAGTTACTTGAAAAGGGGGTGAATGATAGGGGAAGGGTTGCAAATGATGTCGAGACAGAGCAGATCATCCTTGATGAAGAAGCTGGTTCATGCAAGGGAAAAATGAGTTCTGTTGTCCAGATGCGTGGCTCAATTCCCCTCTTTTGGTCACAAGAAGCTTCAAGATTTAGTCCTAAACCTGATATAATCTTACAGAGATATGATCCTACATATCAGGCTACCAAATTACATTTTGAAGACCTGGCAAGGAGATATGGCAACCCAATTATTGTGCTTAATTTAATCAAGACTGTTGAAAAGAGGCCTCGAGAAATGATGCTGAGGCGTGAGTTCACACACGCGGTTGGGTATTTGAACCAAATTCTTTCTGAAGAAAACCATCTTAAATTCATCCACTGGGACTTTCACAAGTTTGCAAAAAGCAAGTCTGCCAACGTTTTAGCAGTTCTGGGTGCTGTAGCAAGTGAAGCACTTGATTTGACCGGATTTTACTTCAGTGGAAAACCTAGCGTTGTTAAACGGAGGCTCAATCAAATAAGTCGAACAAGTACCGGAAGGGAAGCGTCTCTTAGAGATCTGAGAGCCAGTTCTGGGGACCTCCCAAGGTTTGGGAGCAGTAATGAAACTCTAAATTCTGCTGTTACTCGAGATAGAGATACTGATCCTGGTCAACCAAAGAAAAATGCTAATTCTGGTGGTGAACCACCACACTTTCAAAGTGGGGTTTTGCGCACAAACTGCATCGATTGCTTGGATCGTACAAATGTTGCCCAGTATGCTTATGGCCTTGCAGCTTTAGGTCGCCAACTTCATGCAATGGGGTTGACGAACATACCAAAAGTTGATCCTGATAGCACTATTGCTGCAGCTCTCATGGATATGTATCAAAGCATGGGAGATGCTCTTGCCCAGCAATATGGTGGTTCTGCAGCTCACAACACTGTATTTCCTGAGAGACAAGGAAAGTGGAAAGCCACAACCCAATCAAGAGAGTTTCTAAAGTCTATCAAGCGATACTACAGCAATGCTTACACTGATGGTGAAAAGCAAGATGCAATAAATTTATTTTTGGGTTACTTTAAACCACAGGAAGGGAAACCTGCTCTCTGGGAGCTGGATTCTGATTATTATCTTCATGTATCTGGGCTTGATGACCTTCTTCCTGATTTGTGTTCACAGGCCAATGAAATGCACTCAGGAGCATTGGGAAACACTCTTGCCCCTATTCCAGCTTGCAAAGAAGACTTTTTGAGGATGAAGCTGACATCCTTTGACAAATTGATTGAAAGAACGTGCAGTTCAATAAAGAATGTAAGACTTTGCAGCGAACCAGACCAGAGACCTGGTGGTGCTACTGCAAATTCTAGTGTGGCACCTGATGCAGCTGAAATACAGTTGAAAAGCCCAAATTGGCTTTTTGGACAGAGAAAGTATGAAGAAAGTGGATCTGCACCAAAAGTTACTTCACATGAAATCATAAATGGAGGATCCTATAATGACACAGGAGTTGATGGCTATTGTGACCTAAATTGGCTTTCTTCTGGTGGCAATGATAATGAAGAGGATATCTTCCTGAGGTACCTTTCAATGACATCAGTTGATGAAGCCAACGGGTGGTATGGTGGGACTCTGCTAGGTGATCAAGATGAAAGCAGTGAGATATATAAGCACTATGCTGAGTTATGTCAGGGCCCTGCCATTGAGCCATTCCAAAATGATCGGGAGAGAGAGAAGCACTATGCCGATGCTCTTCACATGGGAACAATAAATATTCTAGATGATGCTGCTGTTGAGGCAGAGATGGAAGCAGCTCTCAAGGAGTACTATCAAATTGGAAGTGATCTTGTGAGCATCCCTCCAGCTTGTAAATCATTTGCTGAAGATCCGAGTTGGTTGACGAGATGGATTATTGGGGAAGAGAAGGTGCAAAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000902 GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005794 GO:0005811 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006996 GO:0007010 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0008610 GO:0008654 GO:0009058 GO:0009653 GO:0009826 GO:0009832 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031901 GO:0031902 GO:0031982 GO:0031984 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034595 GO:0034596 GO:0036092 GO:0040007 GO:0042546 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0043813 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046474 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048589 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0060284 GO:0060560 GO:0065007 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0090407 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0106018 GO:0120035 GO:1901576 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

923

Amino Acids

103.93

Weight (kDa)

6.35

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 110 - 416 1.4e-74 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1565
Acc36I ACCTGC 1 cut(s) 1942
Acc65I GGTACC 1 cut(s) 2412
AccB1I GGYRCC 2 cut(s) 2206, 2412
AccB7I CCANNNNNTGG 2 cut(s) 1442, 2448
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 1175
AciI CCGC 9 cut(s) 48, 51, 115, 136, 248, 411, 458, 505, 1175
AclI AACGTT 1 cut(s) 1269
AclWI GGATC 9 cut(s) 224, 1031, 1490, 1596, 1673, 2287, 2316, 2329, 2711
AcsI RAATTY 7 cut(s) 19, 110, 1194, 1220, 1465, 1894, 2194
AcuI CTGAAG 6 cut(s) 206, 254, 742, 1224, 1312, 2733
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 7 cut(s) 35, 509, 711, 1384, 1594, 2414, 2657
AfiI CCNNNNNNNGG 6 cut(s) 135, 978, 1425, 1442, 1925, 2448
AgeI ACCGGT 1 cut(s) 195
AjnI CCWGG 3 cut(s) 1074, 1498, 2176
AjuI GAANNNNNNNTTGG 4 cut(s) 533, 565, 2281, 2313
Alw26I GTCTC 4 cut(s) 881, 1404, 1783, 2167
AlwI GGATC 9 cut(s) 224, 1031, 1490, 1596, 1673, 2287, 2316, 2329, 2711
Ama87I CYCGRG 2 cut(s) 1140, 1479
AoxI GGCC 5 cut(s) 304, 1136, 1618, 2013, 2524
ApaI GGGCCC 1 cut(s) 2528
ApeKI GCWGC 9 cut(s) 502, 1625, 1697, 1700, 1763, 2157, 2216, 2618, 2642
ApoI RAATTY 7 cut(s) 19, 110, 1194, 1220, 1465, 1894, 2194
ArsI GACNNNNNNTTYG 2 cut(s) 1244, 1276
AsiGI ACCGGT 1 cut(s) 195
Asp718I GGTACC 1 cut(s) 2412
AspLEI GCGC 3 cut(s) 30, 783, 1566
AspS9I GGNCC 4 cut(s) 42, 1429, 2524, 2525
AsuHPI GGTGA 5 cut(s) 620, 865, 1544, 1886, 2483
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 2 cut(s) 1140, 1479
AvaII GGWCC 2 cut(s) 42, 1429
AxyI CCTNAGG 1 cut(s) 2408
BaeGI GKGCMC 1 cut(s) 2528
BamHI GGATCC 1 cut(s) 2321
BanI GGYRCC 2 cut(s) 2206, 2412
BanII GRGCYC 1 cut(s) 2528
BarI GAAGNNNNNNTAC 2 cut(s) 17, 49
BbsI GAAGAC 2 cut(s) 1077, 2082
BbvCI CCTCAGC 1 cut(s) 1154
BbvI GCAGC 9 cut(s) 489, 1637, 1684, 1712, 1775, 2169, 2228, 2605, 2654
BccI CCATC 5 cut(s) 1221, 1865, 2339, 2629, 2730
BciT130I CCWGG 3 cut(s) 1076, 1500, 2178
BclI TGATCA 1 cut(s) 2473
BcoDI GTCTC 4 cut(s) 881, 1404, 1783, 2167
BfaI CTAG 6 cut(s) 732, 777, 1341, 2199, 2468, 2609
BfmI CTRYAG 6 cut(s) 175, 553, 1290, 1698, 1761, 1852
BfoI RGCGCY 2 cut(s) 31, 784
BfuAI ACCTGC 1 cut(s) 1942
BglII AGATCT 1 cut(s) 1408
BmcAI AGTACT 1 cut(s) 2657
Bme1390I CCNGG 3 cut(s) 1076, 1500, 2178
Bme18I GGWCC 2 cut(s) 42, 1429
BmeT110I CYCGRG 2 cut(s) 1140, 1479
BmgT120I GGNCC 4 cut(s) 42, 1429, 2524, 2525
BmiI GGNNCC 6 cut(s) 204, 1430, 2208, 2323, 2414, 2526
BmrFI CCNGG 3 cut(s) 1076, 1500, 2178
BmrI ACTGGG 2 cut(s) 1240, 1600
BmuI ACTGGG 2 cut(s) 1240, 1600
BpiI GAAGAC 2 cut(s) 1077, 2082
BplI GAGNNNNNCTC 2 cut(s) 2674, 2706
BpmI CTGGAG 1 cut(s) 2676
Bpu10I CCTNAGC 1 cut(s) 1154
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 2 cut(s) 658, 2633
Bsa29I ATCGAT 2 cut(s) 279, 1578
BsaBI GATNNNNATC 3 cut(s) 1494, 1716, 1962
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 2167
BsaJI CCNNGG 2 cut(s) 321, 1436
BsaWI WCCGGW 6 cut(s) 39, 195, 232, 299, 1316, 1385
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 978, 1425, 1442, 1925, 2448
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 3 cut(s) 1235, 1419, 1606
Bse21I CCTNAGG 1 cut(s) 2408
Bse3DI GCAATG 4 cut(s) 830, 1656, 1864, 2389
Bse8I GATNNNNATC 3 cut(s) 1494, 1716, 1962
BseBI CCWGG 3 cut(s) 1076, 1500, 2178
BseCI ATCGAT 2 cut(s) 279, 1578
BseDI CCNNGG 2 cut(s) 321, 1436
BseGI GGATG 8 cut(s) 214, 347, 624, 900, 1224, 2094, 2099, 2685
BseJI GATNNNNATC 3 cut(s) 1494, 1716, 1962
BseLI CCNNNNNNNGG 6 cut(s) 135, 978, 1425, 1442, 1925, 2448
BseMI GCAATG 4 cut(s) 830, 1656, 1864, 2389
BseMII CTCAG 6 cut(s) 1145, 1403, 1776, 2043, 2399, 2502
BseNI ACTGG 3 cut(s) 1235, 1419, 1606
BseSI GKGCMC 1 cut(s) 2528
BseXI GCAGC 9 cut(s) 489, 1637, 1684, 1712, 1775, 2169, 2228, 2605, 2654
BseYI CCCAGC 1 cut(s) 1743
BsgI GTGCAG 2 cut(s) 2149, 2268
Bsh1236I CGCG 1 cut(s) 1175
Bsh1285I CGRYCG 2 cut(s) 160, 200
BshFI GGCC 5 cut(s) 306, 1138, 1620, 2015, 2526
BshNI GGYRCC 2 cut(s) 2206, 2412
BshTI ACCGGT 1 cut(s) 195
BshVI ATCGAT 2 cut(s) 279, 1578
BsiEI CGRYCG 2 cut(s) 160, 200
BsiHKCI CYCGRG 2 cut(s) 1140, 1479
BsiSI CCGG 8 cut(s) 40, 196, 206, 233, 300, 339, 1317, 1386
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 5 cut(s) 767, 814, 1247, 1442, 2473
BslI CCNNNNNNNGG 6 cut(s) 135, 978, 1425, 1442, 1925, 2448
BsmAI GTCTC 4 cut(s) 881, 1404, 1783, 2167
BsmBI CGTCTC 1 cut(s) 1404
BsmFI GGGAC 5 cut(s) 767, 814, 1247, 1442, 2473
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 5 cut(s) 306, 1138, 1620, 2015, 2526
Bso31I GGTCTC 1 cut(s) 2167
BsoBI CYCGRG 2 cut(s) 1140, 1479
Bsp119I TTCGAA 1 cut(s) 23
Bsp120I GGGCCC 1 cut(s) 2524
Bsp1286I GDGCHC 1 cut(s) 2528
BspACI CCGC 9 cut(s) 48, 51, 115, 136, 248, 411, 458, 505, 1175
BspANI GGCC 5 cut(s) 306, 1138, 1620, 2015, 2526
BspCNI CTCAG 6 cut(s) 1146, 1404, 1777, 2042, 2400, 2503
BspDI ATCGAT 2 cut(s) 279, 1578
BspFNI CGCG 1 cut(s) 1175
BspLI GGNNCC 6 cut(s) 204, 1430, 2208, 2323, 2414, 2526
BspMAI CTGCAG 2 cut(s) 1702, 1765
BspMI ACCTGC 1 cut(s) 1942
BspPI GGATC 9 cut(s) 224, 1031, 1490, 1596, 1673, 2287, 2316, 2329, 2711
BspQI GCTCTTC 1 cut(s) 2589
BspT104I TTCGAA 1 cut(s) 23
BspT107I GGYRCC 2 cut(s) 2206, 2412
BspTNI GGTCTC 1 cut(s) 2167
BsrDI GCAATG 4 cut(s) 830, 1656, 1864, 2389
BsrFI RCCGGY 1 cut(s) 195
BsrI ACTGG 3 cut(s) 1235, 1419, 1606
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 2 cut(s) 321, 1436
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 2 cut(s) 321, 1436
Bst2UI CCWGG 3 cut(s) 1076, 1500, 2178
Bst4CI ACNGT 3 cut(s) 1126, 1777, 2229
Bst6I CTCTTC 3 cut(s) 2388, 2589, 2745
BstACI GRCGYC 1 cut(s) 81
BstAPI GCANNNNNTGC 4 cut(s) 583, 1572, 1581, 2213
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 2068
BstDEI CTNAG 8 cut(s) 492, 1154, 1403, 1412, 1785, 2029, 2408, 2511
BstF5I GGATG 8 cut(s) 214, 347, 624, 900, 1224, 2094, 2099, 2685
BstFNI CGCG 1 cut(s) 1175
BstH2I RGCGCY 2 cut(s) 31, 784
BstHHI GCGC 3 cut(s) 30, 783, 1566
BstMAI GTCTC 4 cut(s) 881, 1404, 1783, 2167
BstMCI CGRYCG 2 cut(s) 160, 200
BstNI CCWGG 3 cut(s) 1076, 1500, 2178
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 3 cut(s) 1074, 1498, 2176
BstSFI CTRYAG 6 cut(s) 175, 553, 1290, 1698, 1761, 1852
BstSLI GKGCMC 1 cut(s) 2528
BstUI CGCG 1 cut(s) 1175
BstV1I GCAGC 9 cut(s) 489, 1637, 1684, 1712, 1775, 2169, 2228, 2605, 2654
BstV2I GAAGAC 2 cut(s) 1077, 2082
BstX2I RGATCY 5 cut(s) 229, 1408, 2279, 2321, 2716
BstXI CCANNNNNNTGG 3 cut(s) 959, 1751, 2177
BstYI RGATCY 5 cut(s) 229, 1408, 2279, 2321, 2716
Bsu15I ATCGAT 2 cut(s) 279, 1578
Bsu36I CCTNAGG 1 cut(s) 2408
BsuRI GGCC 5 cut(s) 306, 1138, 1620, 2015, 2526
BsuTUI ATCGAT 2 cut(s) 279, 1578
BtsCI GGATG 8 cut(s) 214, 347, 624, 900, 1224, 2094, 2099, 2685
BtsI GCAGTG 1 cut(s) 2494
BtsIMutI CAGTG 6 cut(s) 766, 1228, 1336, 1773, 1866, 2494
BveI ACCTGC 1 cut(s) 1942
Cac8I GCNNGC 1 cut(s) 2068
CfoI GCGC 3 cut(s) 30, 783, 1566
Cfr10I RCCGGY 1 cut(s) 195
Cfr13I GGNCC 4 cut(s) 42, 1429, 2524, 2525
ClaI ATCGAT 2 cut(s) 279, 1578
CseI GACGC 3 cut(s) 89, 377, 1386
CsiI ACCWGGT 1 cut(s) 2176
Csp6I GTAC 7 cut(s) 34, 508, 710, 1383, 1593, 2413, 2656
CspAI ACCGGT 1 cut(s) 195
CspCI CAANNNNNGTGG 2 cut(s) 1781, 1816
CviQI GTAC 7 cut(s) 34, 508, 710, 1383, 1593, 2413, 2656
DdeI CTNAG 8 cut(s) 492, 1154, 1403, 1412, 1785, 2029, 2408, 2511
DraI TTTAAA 1 cut(s) 1915
Eam1104I CTCTTC 3 cut(s) 2388, 2589, 2745
EarI CTCTTC 3 cut(s) 2388, 2589, 2745
EciI GGCGGA 2 cut(s) 37, 104
Eco130I CCWWGG 2 cut(s) 321, 1436
Eco147I AGGCCT 2 cut(s) 306, 1138
Eco24I GRGCYC 1 cut(s) 2528
Eco31I GGTCTC 1 cut(s) 2167
Eco32I GATATC 1 cut(s) 2401
Eco47I GGWCC 2 cut(s) 42, 1429
Eco57I CTGAAG 6 cut(s) 206, 254, 742, 1224, 1312, 2733
Eco81I CCTNAGG 1 cut(s) 2408
Eco88I CYCGRG 2 cut(s) 1140, 1479
EcoO109I RGGNCCY 3 cut(s) 1429, 2524, 2525
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 3 cut(s) 1074, 1498, 2176
EcoRV GATATC 1 cut(s) 2401
EcoT14I CCWWGG 2 cut(s) 321, 1436
EcoT22I ATGCAT 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 2528
ErhI CCWWGG 2 cut(s) 321, 1436
Esp3I CGTCTC 1 cut(s) 1404
FalI AAGNNNNNCTT 2 cut(s) 1386, 1418
FaqI GGGAC 5 cut(s) 767, 814, 1247, 1442, 2473
FbaI TGATCA 1 cut(s) 2473
FblI GTMKAC 1 cut(s) 72
FokI GGATG 8 cut(s) 221, 354, 631, 887, 1211, 2086, 2101, 2672
FriOI GRGCYC 1 cut(s) 2528
FspBI CTAG 6 cut(s) 732, 777, 1341, 2199, 2468, 2609
FspI TGCGCA 1 cut(s) 1565
GlaI GCGC 3 cut(s) 29, 782, 1565
GsaI CCCAGC 1 cut(s) 1747
GsuI CTGGAG 1 cut(s) 2676
HaeII RGCGCY 2 cut(s) 31, 784
HaeIII GGCC 5 cut(s) 306, 1138, 1620, 2015, 2526
HapII CCGG 8 cut(s) 40, 196, 206, 233, 300, 339, 1317, 1386
HgaI GACGC 3 cut(s) 89, 377, 1386
HhaI GCGC 3 cut(s) 30, 783, 1566
Hin1I GRCGYC 1 cut(s) 81
Hin6I GCGC 3 cut(s) 28, 781, 1564
HinP1I GCGC 3 cut(s) 28, 781, 1564
HincII GTYRAC 4 cut(s) 73, 1505, 1659, 2730
HindII GTYRAC 4 cut(s) 73, 1505, 1659, 2730
HindIII AAGCTT 2 cut(s) 515, 990
HinfI GANTC 8 cut(s) 171, 262, 276, 359, 439, 469, 1952, 2461
HpaII CCGG 8 cut(s) 40, 196, 206, 233, 300, 339, 1317, 1386
HphI GGTGA 5 cut(s) 620, 865, 1544, 1886, 2483
Hpy166II GTNNAC 9 cut(s) 61, 73, 241, 1167, 1505, 1535, 1659, 2009, 2730
Hpy188I TCNGA 6 cut(s) 162, 202, 1204, 1413, 1957, 2721
Hpy8I GTNNAC 9 cut(s) 61, 73, 241, 1167, 1505, 1535, 1659, 2009, 2730
Hpy99I CGWCG 4 cut(s) 74, 83, 320, 393
HpyAV CCTTC 6 cut(s) 608, 861, 1383, 1919, 2000, 2749
HpyCH4III ACNGT 3 cut(s) 1126, 1777, 2229
HpyCH4IV ACGT 3 cut(s) 36, 1269, 2126
HpyF3I CTNAG 8 cut(s) 492, 1154, 1403, 1412, 1785, 2029, 2408, 2511
HpySE526I ACGT 3 cut(s) 36, 1269, 2126
Hsp92I GRCGYC 1 cut(s) 81
HspAI GCGC 3 cut(s) 28, 781, 1564
KpnI GGTACC 1 cut(s) 2416
Ksp22I TGATCA 1 cut(s) 2473
LguI GCTCTTC 1 cut(s) 2589
LmnI GCTCC 5 cut(s) 153, 202, 1446, 1945, 2033
Lsp1109I GCAGC 9 cut(s) 489, 1637, 1684, 1712, 1775, 2169, 2228, 2605, 2654
MabI ACCWGGT 1 cut(s) 2176
MaeI CTAG 6 cut(s) 732, 777, 1341, 2199, 2468, 2609
MaeII ACGT 3 cut(s) 36, 1269, 2126
MaeIII GTNAC 8 cut(s) 379, 463, 837, 981, 1474, 1907, 2293, 2354
MflI RGATCY 5 cut(s) 229, 1408, 2279, 2321, 2716
MhlI GDGCHC 1 cut(s) 2528
MlyI GAGTC 2 cut(s) 448, 2455
MmeI TCCRAC 1 cut(s) 180
Mph1103I ATGCAT 1 cut(s) 347
MseI TTAA 8 cut(s) 348, 723, 818, 1110, 1115, 1218, 1350, 1914
MslI CAYNNNNRTG 1 cut(s) 1712
MspA1I CMGCKG 3 cut(s) 136, 445, 2219
MspI CCGG 8 cut(s) 40, 196, 206, 233, 300, 339, 1317, 1386
MspR9I CCNGG 3 cut(s) 1076, 1500, 2178
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 3 cut(s) 1076, 1500, 2178
MvnI CGCG 1 cut(s) 1175
NlaIV GGNNCC 6 cut(s) 204, 1430, 2208, 2323, 2414, 2526
NmeAIII GCCGAG 2 cut(s) 40, 766
NmuCI GTSAC 3 cut(s) 463, 981, 2354
NsbI TGCGCA 1 cut(s) 1565
NsiI ATGCAT 1 cut(s) 347
NspI RCATGY 1 cut(s) 464
NspV TTCGAA 1 cut(s) 23
PaeR7I CTCGAG 2 cut(s) 1140, 1479
PceI AGGCCT 2 cut(s) 306, 1138
PciSI GCTCTTC 1 cut(s) 2589
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 6 cut(s) 171, 262, 276, 359, 469, 1952
Pfl23II CGTACG 1 cut(s) 33
PflMI CCANNNNNTGG 2 cut(s) 1442, 2448
PinAI ACCGGT 1 cut(s) 195
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 2 cut(s) 447, 2455
PpsI GAGTC 2 cut(s) 447, 2455
PpuMI RGGWCCY 1 cut(s) 1429
Psp1406I AACGTT 1 cut(s) 1269
Psp5II RGGWCCY 1 cut(s) 1429
Psp6I CCWGG 3 cut(s) 1074, 1498, 2176
PspFI CCCAGC 1 cut(s) 1743
PspGI CCWGG 3 cut(s) 1074, 1498, 2176
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 6 cut(s) 204, 1430, 2208, 2323, 2414, 2526
PspOMI GGGCCC 1 cut(s) 2524
PspPI GGNCC 4 cut(s) 42, 1429, 2524, 2525
PspPPI RGGWCCY 1 cut(s) 1429
PstI CTGCAG 2 cut(s) 1702, 1765
PsuI RGATCY 5 cut(s) 229, 1408, 2279, 2321, 2716
PvuI CGATCG 1 cut(s) 160
PvuII CAGCTG 2 cut(s) 445, 2219
RsaI GTAC 7 cut(s) 35, 509, 711, 1384, 1594, 2414, 2657
RsaNI GTAC 7 cut(s) 34, 508, 710, 1383, 1593, 2413, 2656
RseI CAYNNNNRTG 1 cut(s) 1712
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 2589
SaqAI TTAA 8 cut(s) 348, 723, 818, 1110, 1115, 1218, 1350, 1914
Sau96I GGNCC 4 cut(s) 42, 1429, 2524, 2525
ScaI AGTACT 1 cut(s) 2657
SchI GAGTC 2 cut(s) 448, 2455
ScrFI CCNGG 3 cut(s) 1076, 1500, 2178
SduI GDGCHC 1 cut(s) 2528
SexAI ACCWGGT 1 cut(s) 2176
SfcI CTRYAG 6 cut(s) 175, 553, 1290, 1698, 1761, 1852
Sfr274I CTCGAG 2 cut(s) 1140, 1479
SfuI TTCGAA 1 cut(s) 23
SinI GGWCC 2 cut(s) 42, 1429
SlaI CTCGAG 2 cut(s) 1140, 1479
SmiMI CAYNNNNRTG 1 cut(s) 1712
SmlI CTYRAG 4 cut(s) 673, 1140, 1479, 2648
SmoI CTYRAG 4 cut(s) 673, 1140, 1479, 2648
SseBI AGGCCT 2 cut(s) 306, 1138
SsiI CCGC 9 cut(s) 48, 51, 115, 136, 248, 411, 458, 505, 1175
SspI AATATT 1 cut(s) 2605
SspMI CTAG 6 cut(s) 732, 777, 1341, 2199, 2468, 2609
StuI AGGCCT 2 cut(s) 306, 1138
StyD4I CCNGG 3 cut(s) 1074, 1498, 2176
StyI CCWWGG 2 cut(s) 321, 1436
TaaI ACNGT 3 cut(s) 1126, 1777, 2229
TaiI ACGT 3 cut(s) 39, 1272, 2129
TatI WGTACW 2 cut(s) 709, 2655
TauI GCSGC 2 cut(s) 53, 250
TfiI GAWTC 6 cut(s) 171, 262, 276, 359, 469, 1952
Tru1I TTAA 8 cut(s) 348, 723, 818, 1110, 1115, 1218, 1350, 1914
Tru9I TTAA 8 cut(s) 348, 723, 818, 1110, 1115, 1218, 1350, 1914
TscAI CASTG 6 cut(s) 766, 1235, 1336, 1780, 1873, 2494
TseFI GTSAC 3 cut(s) 463, 981, 2354
TseI GCWGC 9 cut(s) 502, 1625, 1697, 1700, 1763, 2157, 2216, 2618, 2642
Tsp45I GTSAC 3 cut(s) 463, 981, 2354
TspGWI ACGGA 2 cut(s) 58, 1369
TspRI CASTG 6 cut(s) 766, 1235, 1336, 1780, 1873, 2494
Van91I CCANNNNNTGG 2 cut(s) 1442, 2448
VpaK11BI GGWCC 2 cut(s) 42, 1429
XapI RAATTY 7 cut(s) 19, 110, 1194, 1220, 1465, 1894, 2194
XbaI TCTAGA 1 cut(s) 2608
XceI RCATGY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 3 cut(s) 1613, 1751, 2177
XhoI CTCGAG 2 cut(s) 1140, 1479
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 6 cut(s) 732, 777, 1341, 2199, 2468, 2609
ZrmI AGTACT 1 cut(s) 2657
Zsp2I ATGCAT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.