MD15G1408200.v1.1

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006129
Physical Location & Seq
Forward (+)
50822926 .. 50834824
11899 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1408200.v1.1.491

Sequence Viewer

Length: 2733 bp
ATGGCGAAACCTGACAAGTCGAAGCCCAATATCCCCCAATACGTCCGTTCCTCCGCCAAAGTCCACCCCTCCAACGACTTCGACGCCGACCCCAACTCCTACTCCCTCGAAAAGTTCAAGCTCTACGAGACCAGACAGCGATTTTACTTGATCGGAAGCGATCGGAACAAGCGGTTCTTCCGGGTCTTGAAAATCGACCGATCGGAGCCCGACGATTTGAATATCAGCGAAGACCCCGTCGTGTACTCGCCGCAGGAGATCAAGAGTCTGCTTCAGCGAATCGCCGAGGGCAATCGCGCCACCGGAGGCCTTACTTTTGTGGCCAAGGTTTATGGGATCGCCGGTTGCATTAAGTTTCTGGAATCGTATTACTTGATTTTGGTCACCAAGCGTCGCCAAATAGGAAGTATATGTGGTCATGCGATTTATAGCATTGATGAGAGCCAGTTGATTACTATTCCGCACGTCTCGATTCAAACCGACATTGCTCATTCTAAAACCGAGTTGCGGTACAAGAAGCTTCTATCCAGTGTTGATTTGACCAAAGATTTTTTCTATAGTTATACGTATCCTATAATGCAGAGCTTGCAAAAAAATGTGTTATCAATGGGTGAAGAAAGGATGCCGTATGATAATATATTTGTATGGAATGCCTATCTAACACAGGCTATTCGATCAAGGTGCAATAACACAATCTGGACAATAGCATTAGTGCATGGCCATTTTAAGCAGATTAGGCTATCAATCTTTGGTAGGGACTTCAGTGTTTCTCTGGTCTCTAGACGCTCTCGGCATTTTGCAGGAACACGTTACTTGAAAAGGGGAGTGAATGATAGGGGAAGGGTTGCAAATGATGTTGAGACAGAGCAGATAATCCTCGATGAAGAAGCTGGATCATGCAAAGGAAAAATGAGTTCTGTTGTCCAGATGCGTGGTTCGATTCCCCTTTTCTGGTCACAAGAAGCTTCAAGATTTAGCCCTAAGCCTGATATTATATTACAGAGATATGACCCCACATATCAGGCTACCAAATTACATTTCGAAGACCTGGCAAGGAGATATGGCAACCCAGTTATTGTGCTTAATTTGATCAAGACTGTCGAAAAAAGGCCTCGAGAAATGATGCTGAGGCGTGAGTTTGCAAATGCAGTTGGGTACTTGAATCAAATATTTTCGGAAGAAAACCATCTTAAGTTTATTCACTGGGACTTTCACAAGTTTGCAAAAAGCAAGTCTGCCAATGTTTTAGCAGTTTTGGGTGCTGTGGCAAGTGAGGCGCTTGACTTGACTGGTTTTTACTACAGTGGCAAACCTAGCGTTGTTAAACGGAGGCCCAATCAAATAAGCCGGACAAGCACGGGAAGGGAAGCTTCTCTGAGAGCAAACTCTGGGGACGTTCCAAGGTTTGGAAGCAGTAATGAAACTCTAAATTCTGCTGTTAATTGGGACAGGGAATCTGATATTAGTCAACAGAAGAAAAGTGATAATTTTAGCGGTGAACCACCACATTTTCAAAGTGGCGTTCTGCGCACGAATTGTATTGATTGTTTGGATCGTACAAATGTTGCCCAGTATGCTTATGGCCTTGCAGCTTTAGGCCGACAACTTCATGCAATGGGTTTGACAAATCTGCCCAAAGTGGATCCTGATAGCAGTATTGCTGCAGCTCTCATGGATATGTACCAGAGCATGGGAGATGCTCTTGCGCAGCAGTATGGTGGCTCTGCAGCTCACAACACTGTATTTACTGAGAGGCAGGGAAAGTGGAAAGCCACAACCCAATCAAGAGAGTTCCTGAAGTCCATCAAGAGATACTACAGCAATGCTTACACTGATGGTGAAAAGCAAGACGCCATAAATTTATTTTTGGGTTACTTCAAACCACAAGACGGGAAAACTGCTCTCTGGGAGCTGGATTCTGATTACTATCTTCATGTATCTGGAATTGATGACCTCCTCGATGTGTGTCCACAAGGAAATGATATGCACTTAGGAGGATTGGGAAACACTCTTGCCCCTATTCCTGCTTGCAGAGAAGACTTTATGAGGATGAAGTTGACATCTTTTGATAAATTGATTGAAAGGACGTGTAGTTCAATAAGGGATGTTAGACTTTGTAGTGAACCAGATCAAAGACCAGGTGGTGGTCCTGCAAATTCTAGTGTGGCGCCTGATGCAGCTGAAATACAGTTGAAAAGCCCAAATTGGCTTTTTGGCCAGAGAAAATATGAAGAAAGTGGCTCTGCACCAAAAGTTACTTCACATGAAATCAGTAATGGAGGATCCCGTAATGAGACAGGGGTTGACGGCTTTTGTGACTTAAATTGGCTTTCTTCTGAAGCCAATGATAGTGAGGAGGATATCTTCCAGAGGTACCTTTCAATGACATCAGTAAATGAAGCCAATGGGTGGTACGGTGGGACGCTTCTAGGTGATCAAGATGAAAACAGTGAGATATACAAGCACTATGCTGAGTTATGTCAGGGCCCTGGCATTGAGCCGTTCAAAAATGATCGTGAGATGGAGCAGCACTATGCTGATGCTCTTCATATGGGAACAATTAACATTGTCGACGATGCTGATGTTGAGGTAGAGATGGAAGCAGCCCTCAAGGAATACGACCAAATTGGTAGTGATCTTGGGAGCATCCCGGCATCATGTAAATTGTTAGCTGAAGATCCGAGCTGGTTGACGAGGTGGATAATTGGGGAAGAGAAGTTGCAGAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000902 GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005794 GO:0005811 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006996 GO:0007010 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0008610 GO:0008654 GO:0009058 GO:0009653 GO:0009826 GO:0009832 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031901 GO:0031902 GO:0031982 GO:0031984 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034595 GO:0034596 GO:0036092 GO:0040007 GO:0042546 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0043813 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046474 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048589 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0060284 GO:0060560 GO:0065007 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0090407 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0106018 GO:0120035 GO:1901576 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

911

Amino Acids

102.72

Weight (kDa)

6.25

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 111 - 407 2.2e-82 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1529, 1707
Acc65I GGTACC 1 cut(s) 2373
AccB1I GGYRCC 2 cut(s) 2167, 2373
AccB7I CCANNNNNTGG 4 cut(s) 1406, 1690, 2144, 2409
AccI GTMKAC 1 cut(s) 2571
AccII CGCG 1 cut(s) 297
AciI CCGC 6 cut(s) 54, 172, 251, 461, 508, 1494
AclWI GGATC 8 cut(s) 344, 901, 1560, 1637, 1650, 2277, 2290, 2672
AcoI YGGCCR 3 cut(s) 321, 718, 2215
AcsI RAATTY 3 cut(s) 1429, 1858, 2155
AcuI CTGAAG 5 cut(s) 257, 745, 1817, 2358, 2694
AcyI GRCGYC 3 cut(s) 84, 1851, 2168
AfaI GTAC 7 cut(s) 245, 512, 1157, 1558, 1682, 2375, 2414
AfiI CCNNNNNNNGG 7 cut(s) 507, 951, 1406, 1690, 1889, 2144, 2409
AflII CTTAAG 1 cut(s) 1190
AflIII ACRYGT 2 cut(s) 806, 2087
AjiI CACGTC 2 cut(s) 466, 2088
AjnI CCWGG 3 cut(s) 1047, 2137, 2488
AjuI GAANNNNNNNTTGG 6 cut(s) 536, 568, 1022, 1054, 2242, 2274
AloI GAACNNNNNNTCC 2 cut(s) 2077, 2109
Alw26I GTCTC 5 cut(s) 122, 472, 781, 854, 2288
AlwI GGATC 8 cut(s) 344, 901, 1560, 1637, 1650, 2277, 2290, 2672
Ama87I CYCGRG 1 cut(s) 1113
AoxI GGCC 9 cut(s) 307, 321, 718, 1109, 1331, 1582, 1597, 2215, 2485
ApaI GGGCCC 1 cut(s) 2489
ApeKI GCWGC 8 cut(s) 1589, 1661, 1664, 1708, 1727, 2177, 2527, 2603
ApoI RAATTY 3 cut(s) 1429, 1858, 2155
ArsI GACNNNNNNTTYG 4 cut(s) 222, 254, 1217, 1249
Asp718I GGTACC 1 cut(s) 2373
AspLEI GCGC 5 cut(s) 299, 1279, 1530, 1708, 2170
AspS9I GGNCC 4 cut(s) 1332, 2147, 2485, 2486
AsuC2I CCSGG 2 cut(s) 182, 2651
AsuHPI GGTGA 5 cut(s) 376, 623, 1508, 1850, 2444
AsuII TTCGAA 1 cut(s) 1041
AvaI CYCGRG 1 cut(s) 1113
AvaII GGWCC 1 cut(s) 2147
BaeGI GKGCMC 1 cut(s) 2489
BalI TGGCCA 3 cut(s) 323, 720, 2217
BamHI GGATCC 2 cut(s) 1642, 2282
BanI GGYRCC 2 cut(s) 2167, 2373
BanII GRGCYC 2 cut(s) 210, 2489
BbsI GAAGAC 3 cut(s) 237, 1050, 2043
BbvCI CCTCAGC 1 cut(s) 1127
BbvI GCAGC 8 cut(s) 1601, 1648, 1676, 1720, 1739, 2189, 2539, 2615
BccI CCATC 5 cut(s) 1194, 1811, 1829, 2515, 2590
BceAI ACGGC 3 cut(s) 610, 2323, 2485
BcgI CGANNNNNNTGC 2 cut(s) 663, 697
BciT130I CCWGG 3 cut(s) 1049, 2139, 2490
BciVI GTATCC 1 cut(s) 579
BclI TGATCA 2 cut(s) 1089, 2434
BcnI CCSGG 2 cut(s) 182, 2651
BcoDI GTCTC 5 cut(s) 122, 472, 781, 854, 2288
BfaI CTAG 4 cut(s) 780, 1314, 2160, 2429
BfmI CTRYAG 5 cut(s) 556, 1300, 1662, 1725, 1816
BfoI RGCGCY 2 cut(s) 1280, 2171
BfrI CTTAAG 1 cut(s) 1190
BfuI GTATCC 1 cut(s) 579
BisI GCNGC 9 cut(s) 251, 1590, 1662, 1665, 1709, 1728, 2178, 2528, 2604
BlsI GCNGC 9 cut(s) 252, 1591, 1663, 1666, 1710, 1729, 2179, 2529, 2605
Bme1390I CCNGG 5 cut(s) 182, 1049, 2139, 2490, 2651
Bme18I GGWCC 1 cut(s) 2147
BmeT110I CYCGRG 1 cut(s) 1113
BmgBI CACGTC 2 cut(s) 466, 2088
BmgT120I GGNCC 4 cut(s) 1332, 2147, 2485, 2486
BmiI GGNNCC 6 cut(s) 207, 1644, 2169, 2284, 2375, 2487
BmrFI CCNGG 5 cut(s) 182, 1049, 2139, 2490, 2651
BmrI ACTGGG 3 cut(s) 1064, 1213, 1564
BmsI GCATC 9 cut(s) 612, 918, 1113, 1687, 2164, 2530, 2566, 2655, 2663
BmuI ACTGGG 3 cut(s) 1064, 1213, 1564
BpiI GAAGAC 3 cut(s) 237, 1050, 2043
Bpu10I CCTNAGC 2 cut(s) 981, 1127
Bpu14I TTCGAA 1 cut(s) 1041
BpuEI CTTGAG 1 cut(s) 2594
BpuMI CCSGG 2 cut(s) 182, 2651
BsaAI YACGTR 1 cut(s) 567
BsaBI GATNNNNATC 1 cut(s) 1926
BsaHI GRCGYC 3 cut(s) 84, 1851, 2168
BsaI GGTCTC 2 cut(s) 122, 781
BsaJI CCNNGG 4 cut(s) 285, 324, 1400, 2488
BsaWI WCCGGW 1 cut(s) 302
BsaXI ACNNNNNCTCC 4 cut(s) 80, 86, 110, 116
Bsc4I CCNNNNNNNGG 7 cut(s) 507, 951, 1406, 1690, 1889, 2144, 2409
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 6 cut(s) 445, 528, 1070, 1208, 1294, 1570
Bse3DI GCAATG 3 cut(s) 483, 1620, 1828
Bse8I GATNNNNATC 1 cut(s) 1926
BseBI CCWGG 3 cut(s) 1049, 2139, 2490
BseDI CCNNGG 4 cut(s) 285, 324, 1400, 2488
BseGI GGATG 4 cut(s) 627, 2055, 2110, 2646
BseJI GATNNNNATC 1 cut(s) 1926
BseLI CCNNNNNNNGG 7 cut(s) 507, 951, 1406, 1690, 1889, 2144, 2409
BseMI GCAATG 3 cut(s) 483, 1620, 1828
BseMII CTCAG 4 cut(s) 1118, 1367, 1740, 2463
BseNI ACTGG 6 cut(s) 445, 528, 1070, 1208, 1294, 1570
BseRI GAGGAG 2 cut(s) 1946, 2369
BseSI GKGCMC 1 cut(s) 2489
BseXI GCAGC 8 cut(s) 1601, 1648, 1676, 1720, 1739, 2189, 2539, 2615
BsgI GTGCAG 1 cut(s) 2229
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 3 cut(s) 163, 199, 203
BshFI GGCC 9 cut(s) 309, 323, 720, 1111, 1333, 1584, 1599, 2217, 2487
BshNI GGYRCC 2 cut(s) 2167, 2373
BsiEI CGRYCG 3 cut(s) 163, 199, 203
BsiHKCI CYCGRG 1 cut(s) 1113
BsiSI CCGG 5 cut(s) 181, 303, 342, 1348, 2651
BslFI GGGAC 5 cut(s) 770, 1220, 1406, 1460, 2434
BslI CCNNNNNNNGG 7 cut(s) 507, 951, 1406, 1690, 1889, 2144, 2409
BsmAI GTCTC 5 cut(s) 122, 472, 781, 854, 2288
BsmBI CGTCTC 1 cut(s) 472
BsmFI GGGAC 5 cut(s) 770, 1220, 1406, 1460, 2434
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 9 cut(s) 309, 323, 720, 1111, 1333, 1584, 1599, 2217, 2487
Bso31I GGTCTC 2 cut(s) 122, 781
BsoBI CYCGRG 1 cut(s) 1113
Bsp119I TTCGAA 1 cut(s) 1041
Bsp120I GGGCCC 1 cut(s) 2485
Bsp1286I GDGCHC 2 cut(s) 210, 2489
BspACI CCGC 6 cut(s) 54, 172, 251, 461, 508, 1494
BspANI GGCC 9 cut(s) 309, 323, 720, 1111, 1333, 1584, 1599, 2217, 2487
BspCNI CTCAG 4 cut(s) 1119, 1368, 1741, 2464
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 6 cut(s) 207, 1644, 2169, 2284, 2375, 2487
BspMAI CTGCAG 2 cut(s) 1666, 1729
BspPI GGATC 8 cut(s) 344, 901, 1560, 1637, 1650, 2277, 2290, 2672
BspQI GCTCTTC 1 cut(s) 2550
BspT104I TTCGAA 1 cut(s) 1041
BspT107I GGYRCC 2 cut(s) 2167, 2373
BspTI CTTAAG 1 cut(s) 1190
BspTNI GGTCTC 2 cut(s) 122, 781
BsrDI GCAATG 3 cut(s) 483, 1620, 1828
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 6 cut(s) 445, 528, 1070, 1208, 1294, 1570
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 4 cut(s) 285, 324, 1400, 2488
BssNI GRCGYC 3 cut(s) 84, 1851, 2168
BssT1I CCWWGG 2 cut(s) 324, 1400
Bst2UI CCWGG 3 cut(s) 1049, 2139, 2490
Bst4CI ACNGT 6 cut(s) 1099, 1304, 1741, 2190, 2417, 2450
Bst6I CTCTTC 2 cut(s) 2550, 2706
BstACI GRCGYC 3 cut(s) 84, 1851, 2168
BstAFI CTTAAG 1 cut(s) 1190
BstAPI GCANNNNNTGC 1 cut(s) 586
BstBAI YACGTR 1 cut(s) 567
BstBI TTCGAA 1 cut(s) 1041
BstC8I GCNNGC 2 cut(s) 587, 2029
BstDEI CTNAG 6 cut(s) 981, 1127, 1376, 1749, 1990, 2472
BstEII GGTNACC 1 cut(s) 382
BstF5I GGATG 4 cut(s) 627, 2055, 2110, 2646
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 2 cut(s) 1280, 2171
BstHHI GCGC 5 cut(s) 299, 1279, 1530, 1708, 2170
BstMAI GTCTC 5 cut(s) 122, 472, 781, 854, 2288
BstMCI CGRYCG 3 cut(s) 163, 199, 203
BstMWI GCNNNNNNNGC 8 cut(s) 586, 736, 1274, 1314, 1353, 1527, 1574, 2174
BstNI CCWGG 3 cut(s) 1049, 2139, 2490
BstPI GGTNACC 1 cut(s) 382
BstSCI CCNGG 5 cut(s) 180, 1047, 2137, 2488, 2649
BstSFI CTRYAG 5 cut(s) 556, 1300, 1662, 1725, 1816
BstSLI GKGCMC 1 cut(s) 2489
BstSNI TACGTA 1 cut(s) 567
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 8 cut(s) 1601, 1648, 1676, 1720, 1739, 2189, 2539, 2615
BstV2I GAAGAC 3 cut(s) 237, 1050, 2043
BstX2I RGATCY 3 cut(s) 1642, 2282, 2677
BstXI CCANNNNNNTGG 1 cut(s) 932
BstYI RGATCY 3 cut(s) 1642, 2282, 2677
BsuI GTATCC 1 cut(s) 579
BsuRI GGCC 9 cut(s) 309, 323, 720, 1111, 1333, 1584, 1599, 2217, 2487
BtrI CACGTC 2 cut(s) 466, 2088
BtsCI GGATG 4 cut(s) 627, 2055, 2110, 2646
BtsIMutI CAGTG 7 cut(s) 535, 769, 1201, 1309, 1737, 1830, 2455
Cac8I GCNNGC 2 cut(s) 587, 2029
CfoI GCGC 5 cut(s) 299, 1279, 1530, 1708, 2170
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 4 cut(s) 1332, 2147, 2485, 2486
CseI GACGC 5 cut(s) 92, 380, 792, 1859, 2431
CsiI ACCWGGT 1 cut(s) 2137
Csp6I GTAC 7 cut(s) 244, 511, 1156, 1557, 1681, 2374, 2413
CviAII CATG 9 cut(s) 419, 716, 897, 1610, 1672, 1690, 1934, 2264, 2658
CviQI GTAC 7 cut(s) 244, 511, 1156, 1557, 1681, 2374, 2413
DdeI CTNAG 6 cut(s) 981, 1127, 1376, 1749, 1990, 2472
DinI GGCGCC 1 cut(s) 2169
EaeI YGGCCR 3 cut(s) 321, 718, 2215
Eam1104I CTCTTC 2 cut(s) 2550, 2706
EarI CTCTTC 2 cut(s) 2550, 2706
EciI GGCGGA 1 cut(s) 43
Eco105I TACGTA 1 cut(s) 567
Eco130I CCWWGG 2 cut(s) 324, 1400
Eco147I AGGCCT 2 cut(s) 309, 1111
Eco24I GRGCYC 2 cut(s) 210, 2489
Eco31I GGTCTC 2 cut(s) 122, 781
Eco32I GATATC 1 cut(s) 2362
Eco47I GGWCC 1 cut(s) 2147
Eco57I CTGAAG 5 cut(s) 257, 745, 1817, 2358, 2694
Eco88I CYCGRG 1 cut(s) 1113
Eco91I GGTNACC 1 cut(s) 382
EcoO109I RGGNCCY 2 cut(s) 2485, 2486
EcoO65I GGTNACC 1 cut(s) 382
EcoRII CCWGG 3 cut(s) 1047, 2137, 2488
EcoRV GATATC 1 cut(s) 2362
EcoT14I CCWWGG 2 cut(s) 324, 1400
EcoT38I GRGCYC 2 cut(s) 210, 2489
EgeI GGCGCC 1 cut(s) 2169
EheI GGCGCC 1 cut(s) 2169
ErhI CCWWGG 2 cut(s) 324, 1400
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 9 cut(s) 422, 719, 900, 1613, 1675, 1693, 1937, 2267, 2661
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 1354, 1386
FaqI GGGAC 5 cut(s) 770, 1220, 1406, 1460, 2434
FatI CATG 9 cut(s) 418, 715, 896, 1609, 1671, 1689, 1933, 2263, 2657
FauNDI CATATG 1 cut(s) 2550
FbaI TGATCA 2 cut(s) 1089, 2434
FblI GTMKAC 1 cut(s) 2571
Fnu4HI GCNGC 9 cut(s) 251, 1590, 1662, 1665, 1709, 1728, 2178, 2528, 2604
FokI GGATG 4 cut(s) 634, 2062, 2117, 2633
FriOI GRGCYC 2 cut(s) 210, 2489
Fsp4HI GCNGC 9 cut(s) 251, 1590, 1662, 1665, 1709, 1728, 2178, 2528, 2604
FspBI CTAG 4 cut(s) 780, 1314, 2160, 2429
FspI TGCGCA 2 cut(s) 1529, 1707
GlaI GCGC 5 cut(s) 298, 1278, 1529, 1707, 2169
GluI GCNGC 9 cut(s) 251, 1590, 1662, 1665, 1709, 1728, 2178, 2528, 2604
HaeII RGCGCY 2 cut(s) 1280, 2171
HaeIII GGCC 9 cut(s) 309, 323, 720, 1111, 1333, 1584, 1599, 2217, 2487
HapII CCGG 5 cut(s) 181, 303, 342, 1348, 2651
HgaI GACGC 5 cut(s) 92, 380, 792, 1859, 2431
HhaI GCGC 5 cut(s) 299, 1279, 1530, 1708, 2170
Hin1I GRCGYC 3 cut(s) 84, 1851, 2168
Hin1II CATG 9 cut(s) 422, 719, 900, 1613, 1675, 1693, 1937, 2267, 2661
Hin6I GCGC 5 cut(s) 297, 1277, 1528, 1706, 2168
HinP1I GCGC 5 cut(s) 297, 1277, 1528, 1706, 2168
HincII GTYRAC 5 cut(s) 1469, 2058, 2305, 2572, 2691
HindII GTYRAC 5 cut(s) 1469, 2058, 2305, 2572, 2691
HindIII AAGCTT 3 cut(s) 518, 963, 1368
HinfI GANTC 8 cut(s) 265, 279, 362, 472, 940, 1162, 1454, 1916
HpaII CCGG 5 cut(s) 181, 303, 342, 1348, 2651
HphI GGTGA 5 cut(s) 376, 623, 1508, 1850, 2444
Hpy188I TCNGA 9 cut(s) 155, 165, 205, 1177, 1377, 1459, 1921, 2338, 2682
Hpy99I CGWCG 5 cut(s) 86, 215, 242, 396, 2576
HpyAV CCTTC 2 cut(s) 834, 1356
HpyCH4III ACNGT 6 cut(s) 1099, 1304, 1741, 2190, 2417, 2450
HpyCH4IV ACGT 6 cut(s) 42, 465, 566, 808, 1395, 2087
HpyF10VI GCNNNNNNNGC 8 cut(s) 586, 736, 1274, 1314, 1353, 1527, 1574, 2174
HpyF3I CTNAG 6 cut(s) 981, 1127, 1376, 1749, 1990, 2472
HpySE526I ACGT 6 cut(s) 42, 465, 566, 808, 1395, 2087
Hsp92I GRCGYC 3 cut(s) 84, 1851, 2168
Hsp92II CATG 9 cut(s) 422, 719, 900, 1613, 1675, 1693, 1937, 2267, 2661
HspAI GCGC 5 cut(s) 297, 1277, 1528, 1706, 2168
KasI GGCGCC 1 cut(s) 2167
KpnI GGTACC 1 cut(s) 2377
Ksp22I TGATCA 2 cut(s) 1089, 2434
LguI GCTCTTC 1 cut(s) 2550
LmnI GCTCC 4 cut(s) 205, 1909, 2524, 2643
Lsp1109I GCAGC 8 cut(s) 1601, 1648, 1676, 1720, 1739, 2189, 2539, 2615
LweI GCATC 9 cut(s) 612, 918, 1113, 1687, 2164, 2530, 2566, 2655, 2663
MabI ACCWGGT 1 cut(s) 2137
MaeI CTAG 4 cut(s) 780, 1314, 2160, 2429
MaeII ACGT 6 cut(s) 42, 465, 566, 808, 1395, 2087
MaeIII GTNAC 6 cut(s) 382, 809, 954, 1871, 2254, 2315
MflI RGATCY 3 cut(s) 1642, 2282, 2677
MhlI GDGCHC 2 cut(s) 210, 2489
MlsI TGGCCA 3 cut(s) 323, 720, 2217
MluNI TGGCCA 3 cut(s) 323, 720, 2217
Mly113I GGCGCC 1 cut(s) 2168
MlyI GAGTC 1 cut(s) 274
MmeI TCCRAC 1 cut(s) 96
Mox20I TGGCCA 3 cut(s) 323, 720, 2217
MscI TGGCCA 3 cut(s) 323, 720, 2217
MseI TTAA 8 cut(s) 351, 726, 1083, 1191, 1323, 1440, 2321, 2562
MslI CAYNNNNRTG 1 cut(s) 1676
Msp20I TGGCCA 3 cut(s) 323, 720, 2217
MspA1I CMGCKG 1 cut(s) 2180
MspCI CTTAAG 1 cut(s) 1190
MspI CCGG 5 cut(s) 181, 303, 342, 1348, 2651
MspR9I CCNGG 5 cut(s) 182, 1049, 2139, 2490, 2651
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 3 cut(s) 1049, 2139, 2490
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 8 cut(s) 586, 736, 1274, 1314, 1353, 1527, 1574, 2174
NarI GGCGCC 1 cut(s) 2168
NciI CCSGG 2 cut(s) 182, 2651
NdeI CATATG 1 cut(s) 2550
NlaIII CATG 9 cut(s) 422, 719, 900, 1613, 1675, 1693, 1937, 2267, 2661
NlaIV GGNNCC 6 cut(s) 207, 1644, 2169, 2284, 2375, 2487
NmeAIII GCCGAG 2 cut(s) 310, 769
NmuCI GTSAC 3 cut(s) 382, 954, 2315
NsbI TGCGCA 2 cut(s) 1529, 1707
NspV TTCGAA 1 cut(s) 1041
PaeR7I CTCGAG 1 cut(s) 1113
PceI AGGCCT 2 cut(s) 309, 1111
PciSI GCTCTTC 1 cut(s) 2550
PctI GAATGC 1 cut(s) 655
PfeI GAWTC 7 cut(s) 279, 362, 472, 940, 1162, 1454, 1916
PflFI GACNNNGTC 1 cut(s) 236
PflMI CCANNNNNTGG 4 cut(s) 1406, 1690, 2144, 2409
PkrI GCNGC 9 cut(s) 252, 1591, 1663, 1666, 1710, 1729, 2179, 2529, 2605
Ple19I CGATCG 2 cut(s) 163, 203
PleI GAGTC 1 cut(s) 273
PluTI GGCGCC 1 cut(s) 2171
PpsI GAGTC 1 cut(s) 273
Ppu21I YACGTR 1 cut(s) 567
Psp6I CCWGG 3 cut(s) 1047, 2137, 2488
PspEI GGTNACC 1 cut(s) 382
PspGI CCWGG 3 cut(s) 1047, 2137, 2488
PspN4I GGNNCC 6 cut(s) 207, 1644, 2169, 2284, 2375, 2487
PspOMI GGGCCC 1 cut(s) 2485
PspPI GGNCC 4 cut(s) 1332, 2147, 2485, 2486
PstI CTGCAG 2 cut(s) 1666, 1729
PsuI RGATCY 3 cut(s) 1642, 2282, 2677
PsyI GACNNNGTC 1 cut(s) 236
PvuI CGATCG 2 cut(s) 163, 203
PvuII CAGCTG 1 cut(s) 2180
RsaI GTAC 7 cut(s) 245, 512, 1157, 1558, 1682, 2375, 2414
RsaNI GTAC 7 cut(s) 244, 511, 1156, 1557, 1681, 2374, 2413
RseI CAYNNNNRTG 1 cut(s) 1676
SalI GTCGAC 1 cut(s) 2570
SapI GCTCTTC 1 cut(s) 2550
SaqAI TTAA 8 cut(s) 351, 726, 1083, 1191, 1323, 1440, 2321, 2562
SatI GCNGC 9 cut(s) 251, 1590, 1662, 1665, 1709, 1728, 2178, 2528, 2604
Sau96I GGNCC 4 cut(s) 1332, 2147, 2485, 2486
SchI GAGTC 1 cut(s) 274
ScrFI CCNGG 5 cut(s) 182, 1049, 2139, 2490, 2651
SduI GDGCHC 2 cut(s) 210, 2489
SexAI ACCWGGT 1 cut(s) 2137
SfaNI GCATC 9 cut(s) 612, 918, 1113, 1687, 2164, 2530, 2566, 2655, 2663
SfcI CTRYAG 5 cut(s) 556, 1300, 1662, 1725, 1816
SfoI GGCGCC 1 cut(s) 2169
Sfr274I CTCGAG 1 cut(s) 1113
SfuI TTCGAA 1 cut(s) 1041
SinI GGWCC 1 cut(s) 2147
SlaI CTCGAG 1 cut(s) 1113
SmiMI CAYNNNNRTG 1 cut(s) 1676
SmlI CTYRAG 3 cut(s) 1113, 1190, 2609
SmoI CTYRAG 3 cut(s) 1113, 1190, 2609
SnaBI TACGTA 1 cut(s) 567
SseBI AGGCCT 2 cut(s) 309, 1111
SsiI CCGC 6 cut(s) 54, 172, 251, 461, 508, 1494
SspDI GGCGCC 1 cut(s) 2167
SspI AATATT 1 cut(s) 1170
SspMI CTAG 4 cut(s) 780, 1314, 2160, 2429
StuI AGGCCT 2 cut(s) 309, 1111
StyD4I CCNGG 5 cut(s) 180, 1047, 2137, 2488, 2649
StyI CCWWGG 2 cut(s) 324, 1400
TaaI ACNGT 6 cut(s) 1099, 1304, 1741, 2190, 2417, 2450
TaiI ACGT 6 cut(s) 45, 468, 569, 811, 1398, 2090
TaqII GACCGA 1 cut(s) 213
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 253
TfiI GAWTC 7 cut(s) 279, 362, 472, 940, 1162, 1454, 1916
Tru1I TTAA 8 cut(s) 351, 726, 1083, 1191, 1323, 1440, 2321, 2562
Tru9I TTAA 8 cut(s) 351, 726, 1083, 1191, 1323, 1440, 2321, 2562
TscAI CASTG 7 cut(s) 535, 769, 1208, 1309, 1744, 1837, 2455
TseFI GTSAC 3 cut(s) 382, 954, 2315
TseI GCWGC 8 cut(s) 1589, 1661, 1664, 1708, 1727, 2177, 2527, 2603
Tsp45I GTSAC 3 cut(s) 382, 954, 2315
TspGWI ACGGA 2 cut(s) 35, 1342
TspRI CASTG 7 cut(s) 535, 769, 1208, 1309, 1744, 1837, 2455
Tth111I GACNNNGTC 1 cut(s) 236
Van91I CCANNNNNTGG 4 cut(s) 1406, 1690, 2144, 2409
Vha464I CTTAAG 1 cut(s) 1190
VpaK11BI GGWCC 1 cut(s) 2147
XapI RAATTY 3 cut(s) 1429, 1858, 2155
XbaI TCTAGA 1 cut(s) 779
XcmI CCANNNNNNNNNTGG 1 cut(s) 1577
XhoI CTCGAG 1 cut(s) 1113
XmiI GTMKAC 1 cut(s) 2571
XspI CTAG 4 cut(s) 780, 1314, 2160, 2429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.