MD08G1219600.v1.1

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006129
Physical Location & Seq
Forward (+)
28231826 .. 28242695
10870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1219600.v1.1.491

Sequence Viewer

Length: 2718 bp
ATGGCGAAACCTGACAAGTCGAAGCCCAGTATTCCCCAATACGTCCGTTCCTCCGCCAAAGTCCACCCCGACTTCGATGCCGACCCCAACTCCTACTCCCTCGAAAAATTCAAGCTCTACGAGACCAGACAGCGATTTTACTTGATCGGAAGCGATCGGAACAAGCGCTTCTTCCGGGTCTTGAAAATCGACCGCTCGGAGCCCGACGATTTGAATATCAGCGAAGACCCGGTTGTGTACTCGCCGCAGGAGATCAAGAGCCTGCTGCAGCGAATCGCCGAGGGAAATCGCGCCACCGGCGGCCTTACTTTTGTCGCCAAGGTTTTCGGGATCGCCGGTTGCATTAAGTTTCTGGAATCGTATTACTTGATTTTGGTCACCAAGCGTCGCCAAATAGGAAGCATATGTGGTCATGCGATTTATAGCATAGATGAGAGCCAGTTGGTTACTATTCCGCACGTCACGATTCAAACTGATGTTGCTCATTCTAAGACCGAGTTGCGGTACAAGAAACTTCTATCCAGTGTTGATTTGACCAAAGATTTTTTCTATAGTTACACGTATCCTATAATGCAGAGATTGCAAAAGAATGTGTTAGCAATGGGCGAAGAAGGGATGCCGTATGATAATATATTTGTATGGAATGCCTATCTAACACAAGCTATTCGATCAAGGTGCAATAACACAATCTGGACAATAGCATTGGTGCATGGCCATTTTAAGCAGATTAGGCTATCAATCTTTGGTAGGGACTTCAGTGTTTCTCTGGTCTCTAGACGCTCTCGGCATTTCGCAGGAACACGTTACTTGAAAAGGGGAGTGAATGATAGGGGAAGGGTTGCAAATGATGTTGAGACAGAGCAGATAATCCTCGATGAAGAAGCTGGCTCATGCAAAGGAAAAATGAGTTCTGTTGTCCAGATGCGTGGCTCGATTCCCCTTTTCTGGTCACAAGAAGCTTCAAGACTTAGTCCTAAGCCTGATATTATATTACAGAGATATGACCCCACATATCAAGCTACCAAACTACATTTTGAAGACCTGGCAATGAGATATGGCAACCCAGTTATTGTGCTTAATTTGATCAAGACTGTCGAAAAAAGGCCTCGAGAAATGATGCTGAGGCGTGAGTTTGCAAGTGCAGTTGGGTACTTGAATCAAATATTTTCGGAAGAAAACCATCTTAAATTTATTCACTGGGACTTTCACAAGTTTGCAAAAAGCAAGTCTGCCAATGTTTTAGCAGTTTTGGGTGCTGTGGCAAGTGAGGCGCTTGATTTGACTGGTTTTTACTACAGTGGCAAACCTAGCGTTGTTAAACGGAGGCTCAATCAATTAAGTCGGACAAGCACGGGAAGAATTCCAAACGCTGGGGACGTTCCAAGGTTTGAAAGCAGTAATGAAACTCTAAATTCTTCTGTTAATCGGGACAGGGAATCTGATATTATGCAACAGAAGAAAAATGATAATTTTAGTGGTGAACCACCACATTTTCAAAGCGGTGTTCTGCGCACGAATTGTATTGATTGTTTGGATCGTACAAATGTTGCCCAGTATGCTTATGGCCTTGCAGCTCTAGGCCGCCAACTTCATGCAATGGGTTTGACAAATCTGCCCAAAGTGGATCCTGATAGCACTATTGCTGCAGCTCTCATGGATATGTACCAGAGCATGGGAGATGCTCTTGCACAGCAGTATGGTGGCTCTGCAGCTCACAACACCGTATTTCCTGAGAGGCAGGGAAAGTGGAAAGCTACAACCCAATCAAGAGAGTTCCTGAACTCTATTAAGCGATACTACAGCAATGCTTACACTGATGGTGAAAAGCAAGACGCCATAAATTTATTTTTGGGTTACTTCAAACCACAGGAAGGGAAACCTGCTCTCTGGGAGCTGGATTCTGATTACTATCTTCATGTATCTGGAATTGATGACCCTCTCTTTGATGTGAGTTCACAAGGAAATGATACACGCTTAGGAGGAATGGGAGACACTCTTGCCCCTATTCCTGCTTGCAGAGAAGATTTTTTGAGGATGAAGTTGACATCCTTTGATAAATTGATTGAAAGGACATGTAGTTCAATAAAGGATGTTAGACTTTGTAGTGAACCAGATCAAAGACCAGGCGGTAGTACTGCAAATTCAAGTGTGGCGCCTGATGCAGCTGAAATACAGTTGAAAAGCCCAAATTGGCTTTTTGGCCAGAGAAAATACGAAGAAAGTGGCTCTGCACCAAAAGTTACTTCACATGAAATCGGTAATGGAGGATCCCATAACGAGACAGGGGTTGATGGATTTTGTGACTTGAATTGGCTTGCATCTGAAGCCAATGATACTGAGGAGGATATCTTCCAGAGGTACCTTTCAATGACATCAGTAGATGAAACCAATGGGTGGTACGGTGGGACACTGCTAGGTGATCAAGATGAAAACAGTGAGATATATAAGCACTATGCTGAGTTATGTCAGGGCACTGGCATTGAGCCTTTCAAAAATGATGGTGAAATGGAGAAGCACTATGCTGATGCTCTTCATATGGGAACAATTAACATTGTAGATGATGCAGCTGTTGAGGTAGAGATGGAAGCCGCCCTCAAGGAATACGACCAAATTAGTAGTGATCTCGGGAGCATCCCTGCATCGTGTAAATCGTTAGCAGAAGATCCGAGCTGGTTGACGAGGTGGATCATTGGTGAAGAGAAAGTTGAGAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000902 GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005794 GO:0005811 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006996 GO:0007010 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0008610 GO:0008654 GO:0009058 GO:0009653 GO:0009826 GO:0009832 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031901 GO:0031902 GO:0031982 GO:0031984 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034595 GO:0034596 GO:0036092 GO:0040007 GO:0042546 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0043813 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046474 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048589 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0060284 GO:0060560 GO:0065007 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0090407 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0106018 GO:0120035 GO:1901576 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

906

Amino Acids

101.98

Weight (kDa)

6.0

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 109 - 405 5.4e-81 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1511
Acc36I ACCTGC 1 cut(s) 1888
Acc65I GGTACC 1 cut(s) 2358
AccB1I GGYRCC 2 cut(s) 2152, 2358
AccB7I CCANNNNNTGG 3 cut(s) 1370, 1672, 2394
AccBSI CCGCTC 1 cut(s) 195
AccII CGCG 1 cut(s) 291
AclWI GGATC 8 cut(s) 338, 1542, 1619, 1632, 2262, 2275, 2657, 2693
AcoI YGGCCR 2 cut(s) 712, 2200
AcsI RAATTY 6 cut(s) 107, 1187, 1359, 1411, 1840, 2140
AcuI CTGAAG 2 cut(s) 739, 2343
AcyI GRCGYC 2 cut(s) 1833, 2153
AfaI GTAC 8 cut(s) 239, 506, 1151, 1540, 1664, 2134, 2360, 2399
AfeI AGCGCT 1 cut(s) 167
AfiI CCNNNNNNNGG 6 cut(s) 501, 945, 1370, 1672, 1871, 2394
AflIII ACRYGT 3 cut(s) 558, 800, 2072
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 2 cut(s) 1041, 2122
AjuI GAANNNNNNNTTGG 4 cut(s) 530, 562, 2227, 2259
AloI GAACNNNNNNTCC 2 cut(s) 2062, 2094
Alw26I GTCTC 5 cut(s) 116, 775, 848, 1983, 2273
AlwI GGATC 8 cut(s) 338, 1542, 1619, 1632, 2262, 2275, 2657, 2693
Ama87I CYCGRG 2 cut(s) 1107, 2624
Aor51HI AGCGCT 1 cut(s) 167
AoxI GGCC 6 cut(s) 301, 712, 1103, 1564, 1579, 2200
ApeKI GCWGC 8 cut(s) 265, 268, 1571, 1643, 1646, 1709, 2162, 2564
ApoI RAATTY 6 cut(s) 107, 1187, 1359, 1411, 1840, 2140
ArsI GACNNNNNNTTYG 4 cut(s) 1211, 1243, 1925, 1957
Asp718I GGTACC 1 cut(s) 2358
AspLEI GCGC 5 cut(s) 168, 293, 1273, 1512, 2155
AsuC2I CCSGG 2 cut(s) 176, 230
AsuHPI GGTGA 6 cut(s) 370, 1490, 1832, 2429, 2513, 2705
AvaI CYCGRG 2 cut(s) 1107, 2624
BaeGI GKGCMC 1 cut(s) 2474
BalI TGGCCA 2 cut(s) 714, 2202
BamHI GGATCC 2 cut(s) 1624, 2267
BanI GGYRCC 2 cut(s) 2152, 2358
BanII GRGCYC 1 cut(s) 204
BbsI GAAGAC 2 cut(s) 231, 1044
BbvCI CCTCAGC 1 cut(s) 1121
BbvI GCAGC 8 cut(s) 252, 280, 1583, 1630, 1658, 1721, 2174, 2576
BccI CCATC 5 cut(s) 1188, 1811, 2285, 2492, 2575
BceAI ACGGC 1 cut(s) 604
BcgI CGANNNNNNTGC 4 cut(s) 59, 93, 657, 691
BciT130I CCWGG 2 cut(s) 1043, 2124
BciVI GTATCC 1 cut(s) 573
BclI TGATCA 2 cut(s) 1083, 2419
BcnI CCSGG 2 cut(s) 176, 230
BcoDI GTCTC 5 cut(s) 116, 775, 848, 1983, 2273
BfaI CTAG 4 cut(s) 774, 1308, 1577, 2414
BfmI CTRYAG 6 cut(s) 266, 550, 1294, 1644, 1707, 1798
BfoI RGCGCY 3 cut(s) 169, 1274, 2156
BfuAI ACCTGC 1 cut(s) 1888
BfuI GTATCC 1 cut(s) 573
BmcAI AGTACT 1 cut(s) 2134
Bme1390I CCNGG 4 cut(s) 176, 230, 1043, 2124
BmeT110I CYCGRG 2 cut(s) 1107, 2624
BmgBI CACGTC 1 cut(s) 460
BmiI GGNNCC 5 cut(s) 201, 1626, 2154, 2269, 2360
BmrFI CCNGG 4 cut(s) 176, 230, 1043, 2124
BmrI ACTGGG 4 cut(s) 21, 1058, 1207, 1546
BmuI ACTGGG 4 cut(s) 21, 1058, 1207, 1546
BpiI GAAGAC 2 cut(s) 231, 1044
Bpu10I CCTNAGC 3 cut(s) 975, 1121, 1975
BpuEI CTTGAG 1 cut(s) 2579
BpuMI CCSGG 2 cut(s) 176, 230
BsaAI YACGTR 1 cut(s) 561
BsaBI GATNNNNATC 1 cut(s) 1908
BsaHI GRCGYC 2 cut(s) 1833, 2153
BsaI GGTCTC 2 cut(s) 116, 775
BsaJI CCNNGG 3 cut(s) 279, 318, 1382
BsaXI ACNNNNNCTCC 4 cut(s) 74, 80, 104, 110
Bsc4I CCNNNNNNNGG 6 cut(s) 501, 945, 1370, 1672, 1871, 2394
Bse118I RCCGGY 2 cut(s) 296, 335
Bse1I ACTGG 8 cut(s) 27, 439, 522, 1064, 1202, 1288, 1552, 2479
Bse3DI GCAATG 4 cut(s) 606, 1053, 1602, 1810
Bse8I GATNNNNATC 1 cut(s) 1908
BseBI CCWGG 2 cut(s) 1043, 2124
BseDI CCNNGG 3 cut(s) 279, 318, 1382
BseGI GGATG 5 cut(s) 621, 2040, 2045, 2095, 2631
BseJI GATNNNNATC 1 cut(s) 1908
BseLI CCNNNNNNNGG 6 cut(s) 501, 945, 1370, 1672, 1871, 2394
BseMI GCAATG 4 cut(s) 606, 1053, 1602, 1810
BseMII CTCAG 4 cut(s) 1112, 1722, 2328, 2448
BseNI ACTGG 8 cut(s) 27, 439, 522, 1064, 1202, 1288, 1552, 2479
BseRI GAGGAG 1 cut(s) 2354
BseSI GKGCMC 1 cut(s) 2474
BseXI GCAGC 8 cut(s) 252, 280, 1583, 1630, 1658, 1721, 2174, 2576
BseYI CCCAGC 1 cut(s) 1370
BsgI GTGCAG 2 cut(s) 1161, 2214
Bsh1236I CGCG 1 cut(s) 291
Bsh1285I CGRYCG 2 cut(s) 157, 193
BshFI GGCC 6 cut(s) 303, 714, 1105, 1566, 1581, 2202
BshNI GGYRCC 2 cut(s) 2152, 2358
BsiEI CGRYCG 2 cut(s) 157, 193
BsiHKCI CYCGRG 2 cut(s) 1107, 2624
BsiSI CCGG 4 cut(s) 175, 230, 297, 336
BslFI GGGAC 5 cut(s) 764, 1214, 1388, 1442, 2419
BslI CCNNNNNNNGG 6 cut(s) 501, 945, 1370, 1672, 1871, 2394
BsmAI GTCTC 5 cut(s) 116, 775, 848, 1983, 2273
BsmFI GGGAC 5 cut(s) 764, 1214, 1388, 1442, 2419
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 6 cut(s) 303, 714, 1105, 1566, 1581, 2202
Bso31I GGTCTC 2 cut(s) 116, 775
BsoBI CYCGRG 2 cut(s) 1107, 2624
Bsp1286I GDGCHC 2 cut(s) 204, 2474
BspANI GGCC 6 cut(s) 303, 714, 1105, 1566, 1581, 2202
BspCNI CTCAG 4 cut(s) 1113, 1723, 2329, 2449
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 5 cut(s) 201, 1626, 2154, 2269, 2360
BspMAI CTGCAG 3 cut(s) 270, 1648, 1711
BspMI ACCTGC 1 cut(s) 1888
BspPI GGATC 8 cut(s) 338, 1542, 1619, 1632, 2262, 2275, 2657, 2693
BspQI GCTCTTC 1 cut(s) 2535
BspT107I GGYRCC 2 cut(s) 2152, 2358
BspTNI GGTCTC 2 cut(s) 116, 775
BsrBI CCGCTC 1 cut(s) 195
BsrDI GCAATG 4 cut(s) 606, 1053, 1602, 1810
BsrFI RCCGGY 2 cut(s) 296, 335
BsrI ACTGG 8 cut(s) 27, 439, 522, 1064, 1202, 1288, 1552, 2479
BssAI RCCGGY 2 cut(s) 296, 335
BssECI CCNNGG 3 cut(s) 279, 318, 1382
BssNI GRCGYC 2 cut(s) 1833, 2153
BssT1I CCWWGG 2 cut(s) 318, 1382
Bst2UI CCWGG 2 cut(s) 1043, 2124
Bst4CI ACNGT 6 cut(s) 1093, 1298, 1723, 2175, 2402, 2435
Bst6I CTCTTC 2 cut(s) 2535, 2691
BstACI GRCGYC 2 cut(s) 1833, 2153
BstAPI GCANNNNNTGC 1 cut(s) 580
BstBAI YACGTR 1 cut(s) 561
BstC8I GCNNGC 4 cut(s) 263, 886, 2014, 2316
BstDEI CTNAG 8 cut(s) 489, 968, 975, 1121, 1731, 1975, 2337, 2457
BstEII GGTNACC 1 cut(s) 376
BstF5I GGATG 5 cut(s) 621, 2040, 2045, 2095, 2631
BstFNI CGCG 1 cut(s) 291
BstH2I RGCGCY 3 cut(s) 169, 1274, 2156
BstHHI GCGC 5 cut(s) 168, 293, 1273, 1512, 2155
BstMAI GTCTC 5 cut(s) 116, 775, 848, 1983, 2273
BstMCI CGRYCG 2 cut(s) 157, 193
BstMWI GCNNNNNNNGC 8 cut(s) 297, 580, 730, 1268, 1308, 1556, 2159, 2324
BstNI CCWGG 2 cut(s) 1043, 2124
BstNSI RCATGY 1 cut(s) 2076
BstPI GGTNACC 1 cut(s) 376
BstSCI CCNGG 4 cut(s) 174, 228, 1041, 2122
BstSFI CTRYAG 6 cut(s) 266, 550, 1294, 1644, 1707, 1798
BstSLI GKGCMC 1 cut(s) 2474
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 8 cut(s) 252, 280, 1583, 1630, 1658, 1721, 2174, 2576
BstV2I GAAGAC 2 cut(s) 231, 1044
BstX2I RGATCY 3 cut(s) 1624, 2267, 2662
BstXI CCANNNNNNTGG 1 cut(s) 926
BstYI RGATCY 3 cut(s) 1624, 2267, 2662
BsuI GTATCC 1 cut(s) 573
BsuRI GGCC 6 cut(s) 303, 714, 1105, 1566, 1581, 2202
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 5 cut(s) 621, 2040, 2045, 2095, 2631
BtsI GCAGTG 1 cut(s) 2408
BtsIMutI CAGTG 8 cut(s) 529, 763, 1195, 1303, 1812, 2408, 2440, 2472
BveI ACCTGC 1 cut(s) 1888
Cac8I GCNNGC 4 cut(s) 263, 886, 2014, 2316
CfoI GCGC 5 cut(s) 168, 293, 1273, 1512, 2155
Cfr10I RCCGGY 2 cut(s) 296, 335
CseI GACGC 3 cut(s) 374, 786, 1841
Csp6I GTAC 8 cut(s) 238, 505, 1150, 1539, 1663, 2133, 2359, 2398
CviAII CATG 9 cut(s) 413, 710, 891, 1592, 1654, 1672, 1916, 2073, 2249
CviQI GTAC 8 cut(s) 238, 505, 1150, 1539, 1663, 2133, 2359, 2398
DdeI CTNAG 8 cut(s) 489, 968, 975, 1121, 1731, 1975, 2337, 2457
DinI GGCGCC 1 cut(s) 2154
EaeI YGGCCR 2 cut(s) 712, 2200
Eam1104I CTCTTC 2 cut(s) 2535, 2691
EarI CTCTTC 2 cut(s) 2535, 2691
EciI GGCGGA 1 cut(s) 43
Eco130I CCWWGG 2 cut(s) 318, 1382
Eco147I AGGCCT 1 cut(s) 1105
Eco24I GRGCYC 1 cut(s) 204
Eco31I GGTCTC 2 cut(s) 116, 775
Eco32I GATATC 1 cut(s) 2347
Eco47III AGCGCT 1 cut(s) 167
Eco57I CTGAAG 2 cut(s) 739, 2343
Eco88I CYCGRG 2 cut(s) 1107, 2624
Eco91I GGTNACC 1 cut(s) 376
EcoO65I GGTNACC 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 1359
EcoRII CCWGG 2 cut(s) 1041, 2122
EcoRV GATATC 1 cut(s) 2347
EcoT14I CCWWGG 2 cut(s) 318, 1382
EcoT38I GRGCYC 1 cut(s) 204
EgeI GGCGCC 1 cut(s) 2154
EheI GGCGCC 1 cut(s) 2154
ErhI CCWWGG 2 cut(s) 318, 1382
FaeI CATG 9 cut(s) 416, 713, 894, 1595, 1657, 1675, 1919, 2076, 2252
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FaqI GGGAC 5 cut(s) 764, 1214, 1388, 1442, 2419
FatI CATG 9 cut(s) 412, 709, 890, 1591, 1653, 1671, 1915, 2072, 2248
FauNDI CATATG 2 cut(s) 404, 2535
FbaI TGATCA 2 cut(s) 1083, 2419
FokI GGATG 5 cut(s) 628, 2032, 2047, 2102, 2618
FriOI GRGCYC 1 cut(s) 204
FspBI CTAG 4 cut(s) 774, 1308, 1577, 2414
FspI TGCGCA 1 cut(s) 1511
GlaI GCGC 5 cut(s) 167, 292, 1272, 1511, 2154
GsaI CCCAGC 1 cut(s) 1374
HaeII RGCGCY 3 cut(s) 169, 1274, 2156
HaeIII GGCC 6 cut(s) 303, 714, 1105, 1566, 1581, 2202
HapII CCGG 4 cut(s) 175, 230, 297, 336
HgaI GACGC 3 cut(s) 374, 786, 1841
HhaI GCGC 5 cut(s) 168, 293, 1273, 1512, 2155
Hin1I GRCGYC 2 cut(s) 1833, 2153
Hin1II CATG 9 cut(s) 416, 713, 894, 1595, 1657, 1675, 1919, 2076, 2252
Hin6I GCGC 5 cut(s) 166, 291, 1271, 1510, 2153
HinP1I GCGC 5 cut(s) 166, 291, 1271, 1510, 2153
HincII GTYRAC 2 cut(s) 2043, 2676
HindII GTYRAC 2 cut(s) 2043, 2676
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 7 cut(s) 273, 356, 466, 934, 1156, 1436, 1898
HpaII CCGG 4 cut(s) 175, 230, 297, 336
HphI GGTGA 6 cut(s) 370, 1490, 1832, 2429, 2513, 2705
Hpy166II GTNNAC 7 cut(s) 64, 238, 1481, 1955, 2043, 2108, 2676
Hpy188I TCNGA 9 cut(s) 149, 159, 199, 1171, 1344, 1441, 1903, 2323, 2667
Hpy8I GTNNAC 7 cut(s) 64, 238, 1481, 1955, 2043, 2108, 2676
Hpy99I CGWCG 2 cut(s) 209, 390
HpyAV CCTTC 3 cut(s) 605, 828, 1865
HpyCH4III ACNGT 6 cut(s) 1093, 1298, 1723, 2175, 2402, 2435
HpyCH4IV ACGT 5 cut(s) 42, 459, 560, 802, 1377
HpyF10VI GCNNNNNNNGC 8 cut(s) 297, 580, 730, 1268, 1308, 1556, 2159, 2324
HpyF3I CTNAG 8 cut(s) 489, 968, 975, 1121, 1731, 1975, 2337, 2457
HpySE526I ACGT 5 cut(s) 42, 459, 560, 802, 1377
Hsp92I GRCGYC 2 cut(s) 1833, 2153
Hsp92II CATG 9 cut(s) 416, 713, 894, 1595, 1657, 1675, 1919, 2076, 2252
HspAI GCGC 5 cut(s) 166, 291, 1271, 1510, 2153
KasI GGCGCC 1 cut(s) 2152
KpnI GGTACC 1 cut(s) 2362
Ksp22I TGATCA 2 cut(s) 1083, 2419
LguI GCTCTTC 1 cut(s) 2535
LmnI GCTCC 3 cut(s) 199, 1891, 2628
Lsp1109I GCAGC 8 cut(s) 252, 280, 1583, 1630, 1658, 1721, 2174, 2576
MaeI CTAG 4 cut(s) 774, 1308, 1577, 2414
MaeII ACGT 5 cut(s) 42, 459, 560, 802, 1377
MaeIII GTNAC 9 cut(s) 376, 445, 460, 554, 803, 948, 1853, 2239, 2300
MbiI CCGCTC 1 cut(s) 195
MflI RGATCY 3 cut(s) 1624, 2267, 2662
MhlI GDGCHC 2 cut(s) 204, 2474
MlsI TGGCCA 2 cut(s) 714, 2202
MluNI TGGCCA 2 cut(s) 714, 2202
Mly113I GGCGCC 1 cut(s) 2153
MmeI TCCRAC 1 cut(s) 1322
Mox20I TGGCCA 2 cut(s) 714, 2202
MscI TGGCCA 2 cut(s) 714, 2202
MseI TTAA 9 cut(s) 345, 720, 1077, 1185, 1317, 1337, 1422, 1788, 2547
MslI CAYNNNNRTG 1 cut(s) 1658
Msp20I TGGCCA 2 cut(s) 714, 2202
MspA1I CMGCKG 2 cut(s) 2165, 2567
MspI CCGG 4 cut(s) 175, 230, 297, 336
MspR9I CCNGG 4 cut(s) 176, 230, 1043, 2124
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 2 cut(s) 1043, 2124
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 8 cut(s) 297, 580, 730, 1268, 1308, 1556, 2159, 2324
NarI GGCGCC 1 cut(s) 2153
NciI CCSGG 2 cut(s) 176, 230
NdeI CATATG 2 cut(s) 404, 2535
NlaIII CATG 9 cut(s) 416, 713, 894, 1595, 1657, 1675, 1919, 2076, 2252
NlaIV GGNNCC 5 cut(s) 201, 1626, 2154, 2269, 2360
NmeAIII GCCGAG 2 cut(s) 304, 763
NmuCI GTSAC 4 cut(s) 376, 460, 948, 2300
NsbI TGCGCA 1 cut(s) 1511
NspI RCATGY 1 cut(s) 2076
PaeR7I CTCGAG 1 cut(s) 1107
PceI AGGCCT 1 cut(s) 1105
PciI ACATGT 1 cut(s) 2072
PciSI GCTCTTC 1 cut(s) 2535
PcsI WCGNNNNNNNCGW 2 cut(s) 1374, 2648
PctI GAATGC 1 cut(s) 649
PfeI GAWTC 7 cut(s) 273, 356, 466, 934, 1156, 1436, 1898
PflFI GACNNNGTC 1 cut(s) 969
PflMI CCANNNNNTGG 3 cut(s) 1370, 1672, 2394
Ple19I CGATCG 1 cut(s) 157
PluTI GGCGCC 1 cut(s) 2156
Ppu21I YACGTR 1 cut(s) 561
PscI ACATGT 1 cut(s) 2072
Psp6I CCWGG 2 cut(s) 1041, 2122
PspEI GGTNACC 1 cut(s) 376
PspFI CCCAGC 1 cut(s) 1370
PspGI CCWGG 2 cut(s) 1041, 2122
PspN4I GGNNCC 5 cut(s) 201, 1626, 2154, 2269, 2360
PstI CTGCAG 3 cut(s) 270, 1648, 1711
PsuI RGATCY 3 cut(s) 1624, 2267, 2662
PsyI GACNNNGTC 1 cut(s) 969
PvuI CGATCG 1 cut(s) 157
PvuII CAGCTG 2 cut(s) 2165, 2567
RsaI GTAC 8 cut(s) 239, 506, 1151, 1540, 1664, 2134, 2360, 2399
RsaNI GTAC 8 cut(s) 238, 505, 1150, 1539, 1663, 2133, 2359, 2398
RseI CAYNNNNRTG 1 cut(s) 1658
SapI GCTCTTC 1 cut(s) 2535
SaqAI TTAA 9 cut(s) 345, 720, 1077, 1185, 1317, 1337, 1422, 1788, 2547
ScaI AGTACT 1 cut(s) 2134
ScrFI CCNGG 4 cut(s) 176, 230, 1043, 2124
SduI GDGCHC 2 cut(s) 204, 2474
SfcI CTRYAG 6 cut(s) 266, 550, 1294, 1644, 1707, 1798
SfoI GGCGCC 1 cut(s) 2154
Sfr274I CTCGAG 1 cut(s) 1107
SgrAI CRCCGGYG 1 cut(s) 296
SlaI CTCGAG 1 cut(s) 1107
SmiMI CAYNNNNRTG 1 cut(s) 1658
SmlI CTYRAG 2 cut(s) 1107, 2594
SmoI CTYRAG 2 cut(s) 1107, 2594
SseBI AGGCCT 1 cut(s) 1105
SspDI GGCGCC 1 cut(s) 2152
SspI AATATT 1 cut(s) 1164
SspMI CTAG 4 cut(s) 774, 1308, 1577, 2414
StuI AGGCCT 1 cut(s) 1105
StyD4I CCNGG 4 cut(s) 174, 228, 1041, 2122
StyI CCWWGG 2 cut(s) 318, 1382
TaaI ACNGT 6 cut(s) 1093, 1298, 1723, 2175, 2402, 2435
TaiI ACGT 5 cut(s) 45, 462, 563, 805, 1380
TaqI TCGA 9 cut(s) 20, 75, 102, 189, 667, 873, 932, 1095, 1108
TaqII GACCGA 1 cut(s) 509
TatI WGTACW 2 cut(s) 237, 2132
TauI GCSGC 4 cut(s) 247, 303, 1584, 2591
TfiI GAWTC 7 cut(s) 273, 356, 466, 934, 1156, 1436, 1898
Tru1I TTAA 9 cut(s) 345, 720, 1077, 1185, 1317, 1337, 1422, 1788, 2547
Tru9I TTAA 9 cut(s) 345, 720, 1077, 1185, 1317, 1337, 1422, 1788, 2547
TscAI CASTG 8 cut(s) 529, 763, 1202, 1303, 1819, 2415, 2440, 2479
TseFI GTSAC 4 cut(s) 376, 460, 948, 2300
TseI GCWGC 8 cut(s) 265, 268, 1571, 1643, 1646, 1709, 2162, 2564
Tsp45I GTSAC 4 cut(s) 376, 460, 948, 2300
TspDTI ATGAA 9 cut(s) 891, 1416, 1580, 1904, 2051, 2265, 2397, 2442, 2522
TspGWI ACGGA 2 cut(s) 35, 1336
TspRI CASTG 8 cut(s) 529, 763, 1202, 1303, 1819, 2415, 2440, 2479
Tth111I GACNNNGTC 1 cut(s) 969
Van91I CCANNNNNTGG 3 cut(s) 1370, 1672, 2394
XapI RAATTY 6 cut(s) 107, 1187, 1359, 1411, 1840, 2140
XbaI TCTAGA 1 cut(s) 773
XceI RCATGY 1 cut(s) 2076
XcmI CCANNNNNNNNNTGG 1 cut(s) 1559
XhoI CTCGAG 1 cut(s) 1107
XspI CTAG 4 cut(s) 774, 1308, 1577, 2414
ZrmI AGTACT 1 cut(s) 2134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.