FvH4_5g32840

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015639
Physical Location & Seq
Reverse (-)
23709068 .. 23711093
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32840.t1

Sequence Viewer

Length: 1377 bp
ATGGTGGACATGGAGATAAATATAGTTTCCAGAGATACTATCAAACCTGCAGCTGCAGCTACCTCTTCACTTCATCCCCATCAAAAACCTTGCAATCTTTGCATCTTTGATCAGCTCATTCCAACAACTTATGTTCCAATGCTTTTCTTCTACCCCATTATTGATCCCAACTTCAACCTCCCCCAGACACTAGCCCATTTGAAAAACTCACTCTCACATACCCTCACTCAGTACTACCCGTTTTCCGGGCGGACCAAAGACAACCTCTATATTCATGACTTTGATGCTGGTGTCCCCTACCTTGAAGCGCGAGTCGAATGTCGCATGTTCGAGGTTCTTGAGCTTCAAGAAATCGAGTTGCTCAATCGATTTCTTCCGTTCCATCCATTTTGCAAGGAAACTGAATCTCCTGATCTGCTGCCGTTGGTAGCATTTCAGGTCACTGTTTTTGCTTGCGGTGGAATATCAATTGGGATCTCTCTTTCTCACAAGATCTTTGACACACAAACTGCAAATATTTTCCTCAAGTCTTGGGCTTCTGTTTCTCGCAGGGATCATGAAGAAGCAATAACATCAAACCCTAATCTCTGCCAAGCATCGTTAACTTTTCCAGTAAGTGATAATTTACCACAAAAATATCTTGCTTTCATGGAGAGCATGTGGTTCGAGGAAAAGAAGTATGTTACAAGGAGATTTGTGTTTGATGCTAAAGCCATAGCCACATTACGTGAGAAAGCAAAGAGCGAGCTTGTGCCCAATCCAACTCGTGTTGAGGCACTCACTTGTTTTCTTTGGAAACATGCAACATCTGCTTCTAGACATGGTGGTACATCACGAAGAGCATCCGCAGTAGCACATGCAGTGAACTTGAGGCGACGATTGAAGCCACCAATGCCAGATAACGCCATTGGAAATCTATTTTTGTGGGCTACTACAATCTACAATCCTTCTGATACGGGAGAGACTACTGATGAGCTGTGTGATTTGTTGCAGCTGTTACATGAATCTTTCAATGGACTTGACCATGATAGTTTGGATGCATTGCGCGGAGAAGACGGACGTCGAGCCATTTTCGAATACCTCGATCAACTGTTGGAGTGGATGGAAATCTTAGATCCAACCCCAGAAATATATGCGTTCACTAGCTGGATTAACATTCTTGATGAAGTTGATTTTGGCTGGGGGAAGCCATTTTGGATTGGGGTCATGGGAAAAGTTGGACATGCCTTTAGGAACCTTACTGTGTTCGTCAACACACAAAGGGGTAACGGCATTGAAGCATGGGTGACCATGGATGAAAAGCAAATGTCTATAATGGAAAAAGATCCTCAATTTTTAGCATTTGCTTCTCCAAACCCGCGCATTATGAATGTATGA

Protein Analysis

459

Amino Acids

52.19

Weight (kDa)

5.56

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 445 1.5e-71 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1063
Acc36I ACCTGC 1 cut(s) 55
AccII CGCG 3 cut(s) 310, 1047, 1360
AciI CCGC 5 cut(s) 250, 456, 846, 1047, 1358
AclWI GGATC 5 cut(s) 158, 482, 561, 1109, 1319
AcyI GRCGYC 1 cut(s) 1060
AfaI GTAC 2 cut(s) 233, 829
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 7 cut(s) 175, 202, 305, 347, 883, 1012, 1277
AjuI GAANNNNNNNTTGG 2 cut(s) 1158, 1190
AluBI AGCT 8 cut(s) 53, 59, 115, 343, 748, 976, 994, 1146
AluI AGCT 8 cut(s) 53, 59, 115, 343, 748, 976, 994, 1146
Alw26I GTCTC 1 cut(s) 956
AlwI GGATC 5 cut(s) 158, 482, 561, 1109, 1319
ApeKI GCWGC 5 cut(s) 50, 53, 56, 418, 991
AspLEI GCGC 3 cut(s) 310, 1047, 1362
AspS9I GGNCC 1 cut(s) 252
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 1297
AsuII TTCGAA 1 cut(s) 1074
AvaII GGWCC 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 756
BauI CACGAG 1 cut(s) 765
BbsI GAAGAC 1 cut(s) 1059
BbvI GCAGC 5 cut(s) 40, 62, 68, 405, 1003
BccI CCATC 3 cut(s) 87, 390, 1096
BceAI ACGGC 2 cut(s) 406, 1285
BcgI CGANNNNNNTGC 2 cut(s) 646, 680
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 956
BfaI CTAG 3 cut(s) 191, 816, 1143
BfmI CTRYAG 2 cut(s) 48, 54
BfuAI ACCTGC 1 cut(s) 55
BglII AGATCT 1 cut(s) 492
BisI GCNGC 5 cut(s) 51, 54, 57, 419, 992
BlsI GCNGC 5 cut(s) 52, 55, 58, 420, 993
BmcAI AGTACT 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 1235
BmrFI CCNGG 1 cut(s) 247
BmsI GCATC 6 cut(s) 111, 274, 605, 694, 851, 1027
BoxI GACNNNNGTC 1 cut(s) 1059
BpiI GAAGAC 1 cut(s) 1059
Bpu14I TTCGAA 1 cut(s) 1074
BpuEI CTTGAG 3 cut(s) 359, 509, 889
BpuMI CCSGG 1 cut(s) 247
Bsa29I ATCGAT 1 cut(s) 367
BsaAI YACGTR 1 cut(s) 728
BsaBI GATNNNNATC 1 cut(s) 1106
BsaHI GRCGYC 1 cut(s) 1060
BsaJI CCNNGG 1 cut(s) 1290
BsaXI ACNNNNNCTCC 4 cut(s) 391, 421, 644, 674
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 611
Bse3DI GCAATG 1 cut(s) 1040
Bse8I GATNNNNATC 1 cut(s) 1106
BseCI ATCGAT 1 cut(s) 367
BseDI CCNNGG 1 cut(s) 1290
BseGI GGATG 6 cut(s) 73, 382, 842, 1042, 1107, 1300
BseJI GATNNNNATC 1 cut(s) 1106
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 1040
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 1 cut(s) 611
BseSI GKGCMC 1 cut(s) 756
BseXI GCAGC 5 cut(s) 40, 62, 68, 405, 1003
BseYI CCCAGC 1 cut(s) 1179
Bsh1236I CGCG 3 cut(s) 310, 1047, 1360
BshVI ATCGAT 1 cut(s) 367
BsiSI CCGG 1 cut(s) 246
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 956
BsmFI GGGAC 1 cut(s) 278
Bsp119I TTCGAA 1 cut(s) 1074
Bsp1286I GDGCHC 1 cut(s) 756
Bsp143I GATC 9 cut(s) 109, 163, 412, 474, 492, 553, 1084, 1114, 1324
Bsp19I CCATGG 1 cut(s) 1290
BspACI CCGC 5 cut(s) 250, 456, 846, 1047, 1358
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 367
BspFNI CGCG 3 cut(s) 310, 1047, 1360
BspHI TCATGA 2 cut(s) 274, 556
BspLI GGNNCC 1 cut(s) 1235
BspMAI CTGCAG 2 cut(s) 52, 58
BspMI ACCTGC 1 cut(s) 55
BspPI GGATC 5 cut(s) 158, 482, 561, 1109, 1319
BspQI GCTCTTC 1 cut(s) 832
BspT104I TTCGAA 1 cut(s) 1074
BsrDI GCAATG 1 cut(s) 1040
BsrI ACTGG 1 cut(s) 611
BssECI CCNNGG 1 cut(s) 1290
BssMI GATC 9 cut(s) 109, 163, 412, 474, 492, 553, 1084, 1114, 1324
BssNI GRCGYC 1 cut(s) 1060
BssSI CACGAG 1 cut(s) 765
BssT1I CCWWGG 1 cut(s) 1290
Bst2BI CACGAG 1 cut(s) 765
Bst4CI ACNGT 3 cut(s) 445, 1092, 1243
Bst6I CTCTTC 2 cut(s) 70, 832
BstACI GRCGYC 1 cut(s) 1060
BstAPI GCANNNNNTGC 2 cut(s) 99, 809
BstBAI YACGTR 1 cut(s) 728
BstBI TTCGAA 1 cut(s) 1074
BstC8I GCNNGC 2 cut(s) 454, 746
BstDEI CTNAG 2 cut(s) 228, 1111
BstDSI CCRYGG 1 cut(s) 1290
BstEII GGTNACC 1 cut(s) 1285
BstF5I GGATG 6 cut(s) 73, 382, 842, 1042, 1107, 1300
BstFNI CGCG 3 cut(s) 310, 1047, 1360
BstHHI GCGC 3 cut(s) 310, 1047, 1362
BstKTI GATC 9 cut(s) 112, 166, 415, 477, 495, 556, 1087, 1117, 1327
BstMAI GTCTC 1 cut(s) 956
BstMBI GATC 9 cut(s) 109, 163, 412, 474, 492, 553, 1084, 1114, 1324
BstMWI GCNNNNNNNGC 4 cut(s) 56, 99, 809, 892
BstNSI RCATGY 5 cut(s) 328, 661, 803, 860, 1226
BstPAI GACNNNNGTC 1 cut(s) 1059
BstPI GGTNACC 1 cut(s) 1285
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 2 cut(s) 48, 54
BstSLI GKGCMC 1 cut(s) 756
BstUI CGCG 3 cut(s) 310, 1047, 1360
BstV1I GCAGC 5 cut(s) 40, 62, 68, 405, 1003
BstV2I GAAGAC 1 cut(s) 1059
BstX2I RGATCY 4 cut(s) 474, 492, 1114, 1324
BstYI RGATCY 4 cut(s) 474, 492, 1114, 1324
Bsu15I ATCGAT 1 cut(s) 367
BsuTUI ATCGAT 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 1290
BtsCI GGATG 6 cut(s) 73, 382, 842, 1042, 1107, 1300
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 2 cut(s) 441, 867
BveI ACCTGC 1 cut(s) 55
Cac8I GCNNGC 2 cut(s) 454, 746
CciI TCATGA 2 cut(s) 274, 556
CfoI GCGC 3 cut(s) 310, 1047, 1362
Cfr13I GGNCC 1 cut(s) 252
ClaI ATCGAT 1 cut(s) 367
Csp6I GTAC 2 cut(s) 232, 828
CviQI GTAC 2 cut(s) 232, 828
DdeI CTNAG 2 cut(s) 228, 1111
DpnI GATC 9 cut(s) 111, 165, 414, 476, 494, 555, 1086, 1116, 1326
DpnII GATC 9 cut(s) 109, 163, 412, 474, 492, 553, 1084, 1114, 1324
Eam1104I CTCTTC 2 cut(s) 70, 832
EarI CTCTTC 2 cut(s) 70, 832
EciI GGCGGA 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 1290
Eco47I GGWCC 1 cut(s) 252
Eco91I GGTNACC 1 cut(s) 1285
EcoO65I GGTNACC 1 cut(s) 1285
EcoT14I CCWWGG 1 cut(s) 1290
EcoT22I ATGCAT 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 1290
FaqI GGGAC 1 cut(s) 278
FauI CCCGC 1 cut(s) 1365
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 5 cut(s) 51, 54, 57, 419, 992
FokI GGATG 6 cut(s) 60, 369, 829, 1049, 1114, 1307
Fsp4HI GCNGC 5 cut(s) 51, 54, 57, 419, 992
FspBI CTAG 3 cut(s) 191, 816, 1143
GlaI GCGC 3 cut(s) 309, 1046, 1361
GluI GCNGC 5 cut(s) 51, 54, 57, 419, 992
GsaI CCCAGC 1 cut(s) 1183
HapII CCGG 1 cut(s) 246
HhaI GCGC 3 cut(s) 310, 1047, 1362
Hin1I GRCGYC 1 cut(s) 1060
Hin6I GCGC 3 cut(s) 308, 1045, 1360
HinP1I GCGC 3 cut(s) 308, 1045, 1360
HincII GTYRAC 2 cut(s) 603, 1252
HindII GTYRAC 2 cut(s) 603, 1252
HinfI GANTC 3 cut(s) 312, 404, 1004
HpaI GTTAAC 1 cut(s) 603
HpaII CCGG 1 cut(s) 246
HphI GGTGA 1 cut(s) 1297
Hpy166II GTNNAC 5 cut(s) 7, 603, 865, 1140, 1252
Hpy188I TCNGA 1 cut(s) 952
Hpy188III TCNNGA 9 cut(s) 30, 275, 338, 347, 410, 557, 816, 834, 1160
Hpy8I GTNNAC 5 cut(s) 7, 603, 865, 1140, 1252
Hpy99I CGWCG 2 cut(s) 879, 1065
HpyAV CCTTC 1 cut(s) 957
HpyCH4III ACNGT 3 cut(s) 445, 1092, 1243
HpyCH4IV ACGT 2 cut(s) 727, 1060
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 99, 809, 892
HpyF3I CTNAG 2 cut(s) 228, 1111
HpySE526I ACGT 2 cut(s) 727, 1060
Hsp92I GRCGYC 1 cut(s) 1060
HspAI GCGC 3 cut(s) 308, 1045, 1360
Ksp22I TGATCA 1 cut(s) 109
KspAI GTTAAC 1 cut(s) 603
Kzo9I GATC 9 cut(s) 109, 163, 412, 474, 492, 553, 1084, 1114, 1324
LguI GCTCTTC 1 cut(s) 832
Lsp1109I GCAGC 5 cut(s) 40, 62, 68, 405, 1003
LweI GCATC 6 cut(s) 111, 274, 605, 694, 851, 1027
MaeI CTAG 3 cut(s) 191, 816, 1143
MaeII ACGT 2 cut(s) 727, 1060
MaeIII GTNAC 5 cut(s) 439, 682, 996, 1265, 1285
MalI GATC 9 cut(s) 111, 165, 414, 476, 494, 555, 1086, 1116, 1326
MboI GATC 9 cut(s) 109, 163, 412, 474, 492, 553, 1084, 1114, 1324
MboII GAAGA 6 cut(s) 57, 139, 365, 572, 849, 1064
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 4 cut(s) 474, 492, 1114, 1324
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 3 cut(s) 468, 622, 1331
MlyI GAGTC 1 cut(s) 321
MmeI TCCRAC 5 cut(s) 146, 785, 1074, 1142, 1198
Mph1103I ATGCAT 1 cut(s) 1042
MseI TTAA 2 cut(s) 602, 1152
MspA1I CMGCKG 2 cut(s) 53, 994
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 1 cut(s) 247
MunI CAATTG 1 cut(s) 468
MvnI CGCG 3 cut(s) 310, 1047, 1360
MwoI GCNNNNNNNGC 4 cut(s) 56, 99, 809, 892
NciI CCSGG 1 cut(s) 247
NcoI CCATGG 1 cut(s) 1290
NdeII GATC 9 cut(s) 109, 163, 412, 474, 492, 553, 1084, 1114, 1324
NlaIV GGNNCC 1 cut(s) 1235
NmuCI GTSAC 2 cut(s) 439, 1285
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 5 cut(s) 328, 661, 803, 860, 1226
NspV TTCGAA 1 cut(s) 1074
PagI TCATGA 2 cut(s) 274, 556
PciSI GCTCTTC 1 cut(s) 832
PcsI WCGNNNNNNNCGW 1 cut(s) 1080
PfeI GAWTC 2 cut(s) 404, 1004
PkrI GCNGC 5 cut(s) 52, 55, 58, 420, 993
PleI GAGTC 1 cut(s) 320
PpsI GAGTC 1 cut(s) 320
Ppu21I YACGTR 1 cut(s) 728
PshAI GACNNNNGTC 1 cut(s) 1059
PspEI GGTNACC 1 cut(s) 1285
PspFI CCCAGC 1 cut(s) 1179
PspN4I GGNNCC 1 cut(s) 1235
PspPI GGNCC 1 cut(s) 252
PstI CTGCAG 2 cut(s) 52, 58
PsuI RGATCY 4 cut(s) 474, 492, 1114, 1324
PvuII CAGCTG 2 cut(s) 53, 994
RsaI GTAC 2 cut(s) 233, 829
RsaNI GTAC 2 cut(s) 232, 828
SapI GCTCTTC 1 cut(s) 832
SaqAI TTAA 2 cut(s) 602, 1152
SatI GCNGC 5 cut(s) 51, 54, 57, 419, 992
Sau3AI GATC 9 cut(s) 109, 163, 412, 474, 492, 553, 1084, 1114, 1324
Sau96I GGNCC 1 cut(s) 252
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 6 cut(s) 111, 274, 605, 694, 851, 1027
SfcI CTRYAG 2 cut(s) 48, 54
SfuI TTCGAA 1 cut(s) 1074
SinI GGWCC 1 cut(s) 252
SmlI CTYRAG 3 cut(s) 338, 524, 868
SmoI CTYRAG 3 cut(s) 338, 524, 868
Sse9I AATT 3 cut(s) 468, 622, 1331
SsiI CCGC 5 cut(s) 250, 456, 846, 1047, 1358
SspI AATATT 1 cut(s) 517
SspMI CTAG 3 cut(s) 191, 816, 1143
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 1290
TaaI ACNGT 3 cut(s) 445, 1092, 1243
TaiI ACGT 2 cut(s) 730, 1063
TaqI TCGA 8 cut(s) 315, 330, 354, 367, 666, 1063, 1074, 1083
TasI AATT 3 cut(s) 468, 622, 1331
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 2 cut(s) 404, 1004
Tru1I TTAA 2 cut(s) 602, 1152
Tru9I TTAA 2 cut(s) 602, 1152
TscAI CASTG 2 cut(s) 448, 867
TseFI GTSAC 2 cut(s) 439, 1285
TseI GCWGC 5 cut(s) 50, 53, 56, 418, 991
Tsp45I GTSAC 2 cut(s) 439, 1285
TspDTI ATGAA 7 cut(s) 62, 263, 573, 637, 1017, 1179, 1311
TspGWI ACGGA 2 cut(s) 366, 1071
TspRI CASTG 2 cut(s) 448, 867
VpaK11BI GGWCC 1 cut(s) 252
XbaI TCTAGA 1 cut(s) 815
XceI RCATGY 5 cut(s) 328, 661, 803, 860, 1226
XspI CTAG 3 cut(s) 191, 816, 1143
ZraI GACGTC 1 cut(s) 1061
ZrmI AGTACT 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.