RchiOBHm_Chr7g0230711

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0015639
Physical Location & Seq
Reverse (-)
54286053 .. 54288094
2042 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20672

Sequence Viewer

Length: 1377 bp
ATGGTGGACATGGAGGTAAATATAGTTTGCAGAGATACTATCAAACCCGTAGCTGCTTCCTCGTCACTTCATCCCCATCAAAAACCTTGCAATCTTTGCATCTTTGATCAGCTCATTCCAACAACTTATGTGCCTATGCTTTTCTTCTACCCCATTATTGACCCCAACTTCAACGTACCCCAGACCCTAGCCCATTTGAAAACCTCACTGTCACATACCCTCACTCAGTACTACCCATTTTCCGGGCGGACCAAAAACAACCTCTATATTTATAATTTTGATGCTGGTGTCCCCTACGTAGAGGCCCGAGTCAAATGTCACATGTTTGAGTTATTTGAGCTTCAAGAAATCGAGTTGCTCAATCGGTTTGTTCCGTTCCATCCATTTCGCAAGGAAACTGGATCTCCTGATCTGTTGCCCTTGATAGCATTTCAAGTCAGTGTTTTTGCTTGTGGTGGCATATCAATTGGGGTCTCTCTGTCTCACAAGATCTTTGACGCACAAACGGCAAATATTTTCCTCAAGTCTTGGGCTTCTGTTTTTCGTAGGGATCATGAAGAAGCAATACAATCAAACCTTAATCTCTGCCAAGCGTCATCAACTTTTCCAGCAAGTGATAATTTACCACAAAAATATCTTAGTTTCATGGAGAGCACGTGGTTCGAGGAAAAGAAGTATGTTACAAGGAGATTTGTGTTTGATGCTAAAGCCATAGCCACATTACGTGAGAAAGCAAAGAGCGAGCTTGTGCCCAATCCAACTCGTGTTGAGACACTCACTTGTTTTCTTTGGAAACATGCAACATTTGCTTCTAGACATGGTGGTACATCACGAAAAGCATCCGTATTAGCACATGCAGTGAACTTGAGGCGGCGACTGAAGCCACCAATGCCAGATGACGCCATTGGAAATCTATTTTGGTGGGCTACTGCAGTCTACAACACTACTGATAAGGCAGAGACTACTGATGAGTTGTGTGATTTGTTGAAGATATTACATGAATCTCTCAATGGACTTGACCATGATCATTTTTTGGATACATTGCGCGGAGAAGAGGGACGTCGAGCCATTTTTGAATACCTCGATCAATTGTTGGAGGGCATGGATTCCTTTGATCCAACACCAGAAATTTATGCGTTCACTAGCTGGATTAATATTCTCGATGAAGTTGATTTTGGATGGGGAAAGCCAATTTGGATTGGGGTCATGGGAAAAGTTGGACCTGCCTTTAGGAACCTCACTGTGTTCGTCAAAACACAAAGGGATAACGGCATTGAAGCATGGGTGACCATGGATGAAAAGCAAATGTCTATAATGGAAAAAGATCCTCAATTTTTAGCATTTGCTTCACCAAACCCTCGCATTTTAAATGTATGA

Protein Analysis

458

Amino Acids

52.35

Weight (kDa)

6.07

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 445 3.7e-73 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
AatII GACGTC 1 cut(s) 1063
Acc36I ACCTGC 1 cut(s) 1231
AccI GTMKAC 1 cut(s) 936
AccII CGCG 1 cut(s) 1047
AciI CCGC 3 cut(s) 247, 871, 1047
AclWI GGATC 4 cut(s) 409, 558, 1109, 1319
AcsI RAATTY 1 cut(s) 1128
AcuI CTGAAG 1 cut(s) 899
AcvI CACGTG 1 cut(s) 657
AcyI GRCGYC 2 cut(s) 900, 1060
AfaI GTAC 3 cut(s) 177, 230, 826
AfiI CCNNNNNNNGG 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 7 cut(s) 172, 199, 344, 434, 988, 1076, 1277
AjuI GAANNNNNNNTTGG 6 cut(s) 901, 933, 1158, 1177, 1190, 1209
AluBI AGCT 5 cut(s) 53, 112, 340, 745, 1146
AluI AGCT 5 cut(s) 53, 112, 340, 745, 1146
Alw21I GWGCWC 1 cut(s) 656
Alw26I GTCTC 4 cut(s) 478, 486, 764, 953
AlwI GGATC 4 cut(s) 409, 558, 1109, 1319
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 1128
AseI ATTAAT 1 cut(s) 1152
AspLEI GCGC 1 cut(s) 1047
AspS9I GGNCC 3 cut(s) 249, 304, 1220
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 1297, 1341
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 2 cut(s) 249, 1220
BaeGI GKGCMC 1 cut(s) 753
BarI GAAGNNNNNNTAC 2 cut(s) 549, 581
BauI CACGAG 1 cut(s) 762
BbrPI CACGTG 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 656
BbvI GCAGC 1 cut(s) 40
BccI CCATC 3 cut(s) 84, 387, 1173
BceAI ACGGC 2 cut(s) 522, 1285
BcgI CGANNNNNNTGC 2 cut(s) 643, 677
BciVI GTATCC 1 cut(s) 1030
BclI TGATCA 2 cut(s) 106, 1024
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 4 cut(s) 478, 486, 764, 953
BfaI CTAG 3 cut(s) 188, 813, 1143
BfmI CTRYAG 1 cut(s) 930
BfuAI ACCTGC 1 cut(s) 1231
BfuI GTATCC 1 cut(s) 1030
BglII AGATCT 1 cut(s) 489
BisI GCNGC 2 cut(s) 54, 872
BlsI GCNGC 2 cut(s) 55, 873
BmcAI AGTACT 1 cut(s) 230
Bme1390I CCNGG 1 cut(s) 244
Bme18I GGWCC 2 cut(s) 249, 1220
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 3 cut(s) 249, 304, 1220
BmiI GGNNCC 1 cut(s) 1235
BmrFI CCNGG 1 cut(s) 244
BmsI GCATC 4 cut(s) 108, 271, 691, 848
BpuEI CTTGAG 2 cut(s) 506, 886
BpuMI CCSGG 1 cut(s) 244
BsaAI YACGTR 3 cut(s) 298, 657, 725
BsaHI GRCGYC 2 cut(s) 900, 1060
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 1 cut(s) 1290
BsaXI ACNNNNNCTCC 4 cut(s) 388, 418, 641, 671
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 403
Bse3DI GCAATG 1 cut(s) 1040
BseDI CCNNGG 1 cut(s) 1290
BseGI GGATG 5 cut(s) 70, 379, 839, 1184, 1300
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMI GCAATG 1 cut(s) 1040
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 403
BseSI GKGCMC 1 cut(s) 753
BseXI GCAGC 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 1047
BshFI GGCC 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 656
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 2 cut(s) 275, 1071
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 4 cut(s) 478, 486, 764, 953
BsmFI GGGAC 2 cut(s) 275, 1071
BsnI GGCC 1 cut(s) 305
Bso31I GGTCTC 1 cut(s) 478
BsoBI CYCGRG 1 cut(s) 306
Bsp1286I GDGCHC 2 cut(s) 656, 753
Bsp143I GATC 9 cut(s) 106, 401, 409, 489, 550, 1024, 1084, 1114, 1324
Bsp19I CCATGG 1 cut(s) 1290
BspACI CCGC 3 cut(s) 247, 871, 1047
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 1 cut(s) 1047
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 1235
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 1 cut(s) 1231
BspPI GGATC 4 cut(s) 409, 558, 1109, 1319
BspTNI GGTCTC 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 1040
BsrI ACTGG 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 1290
BssMI GATC 9 cut(s) 106, 401, 409, 489, 550, 1024, 1084, 1114, 1324
BssNI GRCGYC 2 cut(s) 900, 1060
BssSI CACGAG 1 cut(s) 762
BssT1I CCWWGG 1 cut(s) 1290
Bst2BI CACGAG 1 cut(s) 762
Bst4CI ACNGT 2 cut(s) 210, 1243
Bst6I CTCTTC 1 cut(s) 1047
BstACI GRCGYC 2 cut(s) 900, 1060
BstAPI GCANNNNNTGC 2 cut(s) 96, 806
BstBAI YACGTR 3 cut(s) 298, 657, 725
BstC8I GCNNGC 1 cut(s) 743
BstDEI CTNAG 2 cut(s) 225, 638
BstDSI CCRYGG 1 cut(s) 1290
BstEII GGTNACC 1 cut(s) 1285
BstF5I GGATG 5 cut(s) 70, 379, 839, 1184, 1300
BstFNI CGCG 1 cut(s) 1047
BstHHI GCGC 1 cut(s) 1047
BstKTI GATC 9 cut(s) 109, 404, 412, 492, 553, 1027, 1087, 1117, 1327
BstMAI GTCTC 4 cut(s) 478, 486, 764, 953
BstMBI GATC 9 cut(s) 106, 401, 409, 489, 550, 1024, 1084, 1114, 1324
BstMWI GCNNNNNNNGC 5 cut(s) 96, 506, 806, 880, 889
BstNSI RCATGY 3 cut(s) 325, 800, 857
BstPI GGTNACC 1 cut(s) 1285
BstSCI CCNGG 1 cut(s) 242
BstSFI CTRYAG 1 cut(s) 930
BstSLI GKGCMC 1 cut(s) 753
BstSNI TACGTA 1 cut(s) 298
BstUI CGCG 1 cut(s) 1047
BstV1I GCAGC 1 cut(s) 40
BstX2I RGATCY 3 cut(s) 401, 489, 1324
BstYI RGATCY 3 cut(s) 401, 489, 1324
BsuI GTATCC 1 cut(s) 1030
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 1290
BtsCI GGATG 5 cut(s) 70, 379, 839, 1184, 1300
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 4 cut(s) 206, 445, 864, 1239
BveI ACCTGC 1 cut(s) 1231
Cac8I GCNNGC 1 cut(s) 743
CciI TCATGA 1 cut(s) 553
CfoI GCGC 1 cut(s) 1047
Cfr13I GGNCC 3 cut(s) 249, 304, 1220
CseI GACGC 3 cut(s) 506, 582, 908
Csp6I GTAC 3 cut(s) 176, 229, 825
CviQI GTAC 3 cut(s) 176, 229, 825
DdeI CTNAG 2 cut(s) 225, 638
DpnI GATC 9 cut(s) 108, 403, 411, 491, 552, 1026, 1086, 1116, 1326
DpnII GATC 9 cut(s) 106, 401, 409, 489, 550, 1024, 1084, 1114, 1324
DraI TTTAAA 1 cut(s) 1368
Eam1104I CTCTTC 1 cut(s) 1047
EarI CTCTTC 1 cut(s) 1047
EciI GGCGGA 1 cut(s) 262
Eco105I TACGTA 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 1290
Eco31I GGTCTC 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 249, 1220
Eco57I CTGAAG 1 cut(s) 899
Eco72I CACGTG 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 1285
EcoO65I GGTNACC 1 cut(s) 1285
EcoT14I CCWWGG 1 cut(s) 1290
ErhI CCWWGG 1 cut(s) 1290
FaqI GGGAC 2 cut(s) 275, 1071
FbaI TGATCA 2 cut(s) 106, 1024
FblI GTMKAC 1 cut(s) 936
Fnu4HI GCNGC 2 cut(s) 54, 872
FokI GGATG 5 cut(s) 57, 366, 826, 1191, 1307
Fsp4HI GCNGC 2 cut(s) 54, 872
FspBI CTAG 3 cut(s) 188, 813, 1143
GlaI GCGC 1 cut(s) 1046
GluI GCNGC 2 cut(s) 54, 872
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 243
HgaI GACGC 3 cut(s) 506, 582, 908
HhaI GCGC 1 cut(s) 1047
Hin1I GRCGYC 2 cut(s) 900, 1060
Hin6I GCGC 1 cut(s) 1045
HinP1I GCGC 1 cut(s) 1045
HinfI GANTC 3 cut(s) 309, 1001, 1106
HpaII CCGG 1 cut(s) 243
HphI GGTGA 2 cut(s) 1297, 1341
Hpy166II GTNNAC 4 cut(s) 7, 862, 937, 1140
Hpy188III TCNNGA 6 cut(s) 344, 407, 554, 813, 831, 1160
Hpy8I GTNNAC 4 cut(s) 7, 862, 937, 1140
Hpy99I CGWCG 1 cut(s) 1065
HpyCH4III ACNGT 2 cut(s) 210, 1243
HpyCH4IV ACGT 5 cut(s) 174, 297, 656, 724, 1060
HpyCH4V TGCA 6 cut(s) 30, 90, 99, 800, 857, 932
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 506, 806, 880, 889
HpyF3I CTNAG 2 cut(s) 225, 638
HpySE526I ACGT 5 cut(s) 174, 297, 656, 724, 1060
Hsp92I GRCGYC 2 cut(s) 900, 1060
HspAI GCGC 1 cut(s) 1045
Ksp22I TGATCA 2 cut(s) 106, 1024
Kzo9I GATC 9 cut(s) 106, 401, 409, 489, 550, 1024, 1084, 1114, 1324
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 4 cut(s) 108, 271, 691, 848
MaeI CTAG 3 cut(s) 188, 813, 1143
MaeII ACGT 5 cut(s) 174, 297, 656, 724, 1060
MaeIII GTNAC 5 cut(s) 63, 210, 317, 679, 1285
MalI GATC 9 cut(s) 108, 403, 411, 491, 552, 1026, 1086, 1116, 1326
MboI GATC 9 cut(s) 106, 401, 409, 489, 550, 1024, 1084, 1114, 1324
MboII GAAGA 4 cut(s) 136, 569, 1000, 1064
MfeI CAATTG 2 cut(s) 465, 1088
MflI RGATCY 3 cut(s) 401, 489, 1324
MhlI GDGCHC 2 cut(s) 656, 753
MluCI AATT 7 cut(s) 274, 465, 619, 1088, 1128, 1191, 1331
MlyI GAGTC 1 cut(s) 318
MmeI TCCRAC 5 cut(s) 143, 782, 1074, 1142, 1198
MseI TTAA 3 cut(s) 579, 1152, 1367
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 244
MunI CAATTG 2 cut(s) 465, 1088
MvnI CGCG 1 cut(s) 1047
MwoI GCNNNNNNNGC 5 cut(s) 96, 506, 806, 880, 889
NciI CCSGG 1 cut(s) 244
NcoI CCATGG 1 cut(s) 1290
NdeII GATC 9 cut(s) 106, 401, 409, 489, 550, 1024, 1084, 1114, 1324
NlaIV GGNNCC 1 cut(s) 1235
NmuCI GTSAC 4 cut(s) 63, 210, 317, 1285
NspI RCATGY 3 cut(s) 325, 800, 857
PagI TCATGA 1 cut(s) 553
PciI ACATGT 1 cut(s) 321
PfeI GAWTC 2 cut(s) 1001, 1106
PkrI GCNGC 2 cut(s) 55, 873
PleI GAGTC 1 cut(s) 317
PmaCI CACGTG 1 cut(s) 657
PmlI CACGTG 1 cut(s) 657
PpsI GAGTC 1 cut(s) 317
Ppu21I YACGTR 3 cut(s) 298, 657, 725
PscI ACATGT 1 cut(s) 321
PshBI ATTAAT 1 cut(s) 1152
PsiI TTATAA 1 cut(s) 273
PspCI CACGTG 1 cut(s) 657
PspEI GGTNACC 1 cut(s) 1285
PspN4I GGNNCC 1 cut(s) 1235
PspPI GGNCC 3 cut(s) 249, 304, 1220
PstI CTGCAG 1 cut(s) 934
PsuI RGATCY 3 cut(s) 401, 489, 1324
RsaI GTAC 3 cut(s) 177, 230, 826
RsaNI GTAC 3 cut(s) 176, 229, 825
SaqAI TTAA 3 cut(s) 579, 1152, 1367
SatI GCNGC 2 cut(s) 54, 872
Sau3AI GATC 9 cut(s) 106, 401, 409, 489, 550, 1024, 1084, 1114, 1324
Sau96I GGNCC 3 cut(s) 249, 304, 1220
ScaI AGTACT 1 cut(s) 230
SchI GAGTC 1 cut(s) 318
ScrFI CCNGG 1 cut(s) 244
SduI GDGCHC 2 cut(s) 656, 753
SfaNI GCATC 4 cut(s) 108, 271, 691, 848
SfcI CTRYAG 1 cut(s) 930
SinI GGWCC 2 cut(s) 249, 1220
SmlI CTYRAG 2 cut(s) 521, 865
SmoI CTYRAG 2 cut(s) 521, 865
SnaBI TACGTA 1 cut(s) 298
Sse9I AATT 7 cut(s) 274, 465, 619, 1088, 1128, 1191, 1331
SsiI CCGC 3 cut(s) 247, 871, 1047
SspI AATATT 2 cut(s) 514, 1156
SspMI CTAG 3 cut(s) 188, 813, 1143
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 1 cut(s) 1290
TaaI ACNGT 2 cut(s) 210, 1243
TaiI ACGT 5 cut(s) 177, 300, 659, 727, 1063
TaqI TCGA 5 cut(s) 351, 663, 1063, 1083, 1161
TasI AATT 7 cut(s) 274, 465, 619, 1088, 1128, 1191, 1331
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 874
TfiI GAWTC 2 cut(s) 1001, 1106
Tru1I TTAA 3 cut(s) 579, 1152, 1367
Tru9I TTAA 3 cut(s) 579, 1152, 1367
TscAI CASTG 4 cut(s) 213, 445, 864, 1246
TseFI GTSAC 4 cut(s) 63, 210, 317, 1285
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 4 cut(s) 63, 210, 317, 1285
TspDTI ATGAA 6 cut(s) 59, 570, 634, 1014, 1179, 1311
TspGWI ACGGA 2 cut(s) 363, 832
TspRI CASTG 4 cut(s) 213, 445, 864, 1246
VpaK11BI GGWCC 2 cut(s) 249, 1220
VspI ATTAAT 1 cut(s) 1152
XapI RAATTY 1 cut(s) 1128
XbaI TCTAGA 1 cut(s) 812
XceI RCATGY 3 cut(s) 325, 800, 857
XmiI GTMKAC 1 cut(s) 936
XspI CTAG 3 cut(s) 188, 813, 1143
ZraI GACGTC 1 cut(s) 1061
ZrmI AGTACT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.