RLG00000001481

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015639
Physical Location & Seq
Forward (+)
16183079 .. 16185080
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001481

Sequence Viewer

Length: 1173 bp
ATGGTGGACGTGGAGGTAAATATAGTTTGCAGAGATACTATGATCAAACCCGTAGCTGCTTCCTTGTCACTTCATCCCCATCAAAAACCTTGCAATCTTTGCATCTTTGATCAGCTCATTCCAACAACTTATGTGCCTATGCTTTTCTTCTACCCCATTATTGACCCCAACTTCAACGAAACTGGATCTCCTGATCTGTTGCCCTTGATAGCATTTCAAGTCACTGTTTTTGCTTGTGGTGGGATATCAATTGGGGTCTCTCTGTCTCACCAGATCTTTGACGCACAAACTGCAAATATTTTCCTCAAGTCTTGGGCTTCTGTTTTTCGTAGGGATCATGAAGAAGCAATACAATCAAACCTTAATCTCTGCCAAGCGTCATCAACTTTTCCAGCAAGTGATAATTTACCACAAAAAAATCCTAGTTTCATGGAGAGCACGTGGTTCGAGGAAAAGAAGTATGTTACAAGGAGATTTGTGTTTGATGCTAAAGCCATAGCCACATTACGTGAGAAAGCAAAGAGCGATCTTGTGCCCAATCCAACTCGTGTTGAGACACTCACTTGTTTTCTTTGGAAACATGCAACATTTGCTTCTAGACATGGACCACCTAATAATTCCCCATCACGAAGAGCATCCGTAGTAGCACATGCAGTGAACTTGAGGTGGCGACTAAAGCCACCAATGCCAGATGACGCCATTGGAAATCTATTTTGGTGGGCTACTGCAGTCTACAACACTACTGGTAAGGCAGAGACTACTGATGAGTTGTGTGATTTGTTGAAGATATTACATGAATCTCTCAATGGATTTGACCATGATCATTTTTTGGATACATTGCGCGGAGAAGAGGGACGTCGAGCCATTTTTGAATACCTCGATCAATTGTTAGAGGGCATGGATTCCTTAGATCCAACACCGGAAATTTATGCTTTCACTAGCTGGATTAATATTCTCGATGAAGTTGATTTTGGATGGGGAAAGCCATTTTGGATTGGGGTCATGGGAAAAGTTGGACCTGCCTTTAGGAACCTCACTGTGTTCGTCAAAACACAAAGGGATAACGGCATTGAAGCATGGGTGACCATGAATGAAAAGCAAATGTCTATAATGGAAAAACATCCTCAATTTTTAGCATTTGCTTCTCCAAACCAGCGCATTTTAAATGTATGA

Protein Analysis

391

Amino Acids

44.24

Weight (kDa)

5.79

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 66 - 377 8.4e-51 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 859
Acc36I ACCTGC 1 cut(s) 1027
AccI GTMKAC 1 cut(s) 732
AccII CGCG 1 cut(s) 843
AciI CCGC 1 cut(s) 843
AclWI GGATC 3 cut(s) 193, 342, 905
AcsI RAATTY 1 cut(s) 924
AcvI CACGTG 1 cut(s) 441
AcyI GRCGYC 2 cut(s) 696, 856
AgsI TTSAA 5 cut(s) 175, 218, 784, 872, 1073
AjiI CACGTC 1 cut(s) 10
AjuI GAANNNNNNNTTGG 6 cut(s) 697, 729, 954, 973, 986, 1005
AluBI AGCT 3 cut(s) 56, 115, 942
AluI AGCT 3 cut(s) 56, 115, 942
Alw21I GWGCWC 1 cut(s) 440
Alw26I GTCTC 4 cut(s) 262, 270, 548, 749
AlwI GGATC 3 cut(s) 193, 342, 905
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 1 cut(s) 924
AseI ATTAAT 1 cut(s) 948
AspLEI GCGC 2 cut(s) 843, 1158
AspS9I GGNCC 2 cut(s) 605, 1016
AsuHPI GGTGA 2 cut(s) 260, 1093
AvaII GGWCC 2 cut(s) 605, 1016
BaeGI GKGCMC 1 cut(s) 537
BarI GAAGNNNNNNTAC 2 cut(s) 333, 365
BauI CACGAG 1 cut(s) 546
BbrPI CACGTG 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 440
BbvI GCAGC 1 cut(s) 43
BccI CCATC 3 cut(s) 87, 631, 969
BceAI ACGGC 1 cut(s) 1081
BcgI CGANNNNNNTGC 2 cut(s) 427, 461
BciVI GTATCC 1 cut(s) 826
BclI TGATCA 3 cut(s) 42, 109, 820
BcoDI GTCTC 4 cut(s) 262, 270, 548, 749
BfaI CTAG 3 cut(s) 423, 597, 939
BfmI CTRYAG 1 cut(s) 726
BfuAI ACCTGC 1 cut(s) 1027
BfuI GTATCC 1 cut(s) 826
BglII AGATCT 1 cut(s) 273
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme18I GGWCC 2 cut(s) 605, 1016
BmgBI CACGTC 1 cut(s) 10
BmgT120I GGNCC 2 cut(s) 605, 1016
BmiI GGNNCC 1 cut(s) 1031
BmsI GCATC 3 cut(s) 111, 475, 644
BpuEI CTTGAG 2 cut(s) 290, 682
BsaAI YACGTR 2 cut(s) 441, 509
BsaHI GRCGYC 2 cut(s) 696, 856
BsaI GGTCTC 1 cut(s) 262
BsaWI WCCGGW 1 cut(s) 919
BsaXI ACNNNNNCTCC 4 cut(s) 172, 202, 425, 455
Bse1I ACTGG 2 cut(s) 187, 748
Bse3DI GCAATG 1 cut(s) 836
BseGI GGATG 4 cut(s) 73, 635, 980, 1120
BseMI GCAATG 1 cut(s) 836
BseNI ACTGG 2 cut(s) 187, 748
BseSI GKGCMC 1 cut(s) 537
BseXI GCAGC 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 843
BsiHKAI GWGCWC 1 cut(s) 440
BsiSI CCGG 1 cut(s) 920
BslFI GGGAC 1 cut(s) 867
BsmAI GTCTC 4 cut(s) 262, 270, 548, 749
BsmFI GGGAC 1 cut(s) 867
Bso31I GGTCTC 1 cut(s) 262
Bsp1286I GDGCHC 2 cut(s) 440, 537
BspACI CCGC 1 cut(s) 843
BspFNI CGCG 1 cut(s) 843
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 1 cut(s) 1031
BspMAI CTGCAG 1 cut(s) 730
BspMI ACCTGC 1 cut(s) 1027
BspPI GGATC 3 cut(s) 193, 342, 905
BspQI GCTCTTC 1 cut(s) 625
BspTNI GGTCTC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 836
BsrI ACTGG 2 cut(s) 187, 748
BssNI GRCGYC 2 cut(s) 696, 856
BssSI CACGAG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 546
Bst4CI ACNGT 2 cut(s) 226, 1039
Bst6I CTCTTC 2 cut(s) 625, 843
BstACI GRCGYC 2 cut(s) 696, 856
BstAPI GCANNNNNTGC 3 cut(s) 99, 290, 590
BstBAI YACGTR 2 cut(s) 441, 509
BstDEI CTNAG 1 cut(s) 907
BstEII GGTNACC 1 cut(s) 1081
BstF5I GGATG 4 cut(s) 73, 635, 980, 1120
BstFNI CGCG 1 cut(s) 843
BstHHI GCGC 2 cut(s) 843, 1158
BstMAI GTCTC 4 cut(s) 262, 270, 548, 749
BstMWI GCNNNNNNNGC 5 cut(s) 99, 290, 590, 676, 685
BstNSI RCATGY 2 cut(s) 584, 653
BstPI GGTNACC 1 cut(s) 1081
BstSFI CTRYAG 1 cut(s) 726
BstSLI GKGCMC 1 cut(s) 537
BstUI CGCG 1 cut(s) 843
BstV1I GCAGC 1 cut(s) 43
BstX2I RGATCY 3 cut(s) 185, 273, 910
BstYI RGATCY 3 cut(s) 185, 273, 910
BsuI GTATCC 1 cut(s) 826
BtrI CACGTC 1 cut(s) 10
BtsCI GGATG 4 cut(s) 73, 635, 980, 1120
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 3 cut(s) 222, 660, 1035
BveI ACCTGC 1 cut(s) 1027
CciI TCATGA 1 cut(s) 337
CfoI GCGC 2 cut(s) 843, 1158
Cfr13I GGNCC 2 cut(s) 605, 1016
CseI GACGC 3 cut(s) 290, 366, 704
DdeI CTNAG 1 cut(s) 907
DraI TTTAAA 1 cut(s) 1164
Eam1104I CTCTTC 2 cut(s) 625, 843
EarI CTCTTC 2 cut(s) 625, 843
Eco31I GGTCTC 1 cut(s) 262
Eco32I GATATC 1 cut(s) 246
Eco47I GGWCC 2 cut(s) 605, 1016
Eco72I CACGTG 1 cut(s) 441
Eco91I GGTNACC 1 cut(s) 1081
EcoO65I GGTNACC 1 cut(s) 1081
EcoRV GATATC 1 cut(s) 246
FaqI GGGAC 1 cut(s) 867
FbaI TGATCA 3 cut(s) 42, 109, 820
FblI GTMKAC 1 cut(s) 732
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 4 cut(s) 60, 622, 987, 1107
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 3 cut(s) 423, 597, 939
GlaI GCGC 2 cut(s) 842, 1157
GluI GCNGC 1 cut(s) 57
HapII CCGG 1 cut(s) 920
HgaI GACGC 3 cut(s) 290, 366, 704
HhaI GCGC 2 cut(s) 843, 1158
Hin1I GRCGYC 2 cut(s) 696, 856
Hin6I GCGC 2 cut(s) 841, 1156
HinP1I GCGC 2 cut(s) 841, 1156
HinfI GANTC 2 cut(s) 797, 902
HpaII CCGG 1 cut(s) 920
HphI GGTGA 2 cut(s) 260, 1093
Hpy166II GTNNAC 3 cut(s) 7, 658, 733
Hpy188III TCNNGA 5 cut(s) 191, 338, 597, 627, 956
Hpy8I GTNNAC 3 cut(s) 7, 658, 733
Hpy99I CGWCG 1 cut(s) 861
HpyCH4III ACNGT 2 cut(s) 226, 1039
HpyCH4IV ACGT 4 cut(s) 9, 440, 508, 856
HpyCH4V TGCA 7 cut(s) 30, 93, 102, 293, 584, 653, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 290, 590, 676, 685
HpyF3I CTNAG 1 cut(s) 907
HpySE526I ACGT 4 cut(s) 9, 440, 508, 856
Hsp92I GRCGYC 2 cut(s) 696, 856
HspAI GCGC 2 cut(s) 841, 1156
Ksp22I TGATCA 3 cut(s) 42, 109, 820
LguI GCTCTTC 1 cut(s) 625
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 3 cut(s) 111, 475, 644
MaeI CTAG 3 cut(s) 423, 597, 939
MaeII ACGT 4 cut(s) 9, 440, 508, 856
MaeIII GTNAC 4 cut(s) 66, 220, 463, 1081
MboII GAAGA 5 cut(s) 139, 353, 642, 796, 860
MfeI CAATTG 2 cut(s) 249, 884
MflI RGATCY 3 cut(s) 185, 273, 910
MhlI GDGCHC 2 cut(s) 440, 537
MluCI AATT 6 cut(s) 249, 403, 616, 884, 924, 1127
MmeI TCCRAC 4 cut(s) 146, 566, 938, 994
MnlI CCTC 9 cut(s) 7, 314, 442, 657, 844, 886, 887, 1043, 1134
MseI TTAA 3 cut(s) 363, 948, 1163
MspI CCGG 1 cut(s) 920
MunI CAATTG 2 cut(s) 249, 884
MvnI CGCG 1 cut(s) 843
MwoI GCNNNNNNNGC 5 cut(s) 99, 290, 590, 676, 685
NlaIV GGNNCC 1 cut(s) 1031
NmuCI GTSAC 3 cut(s) 66, 220, 1081
NspI RCATGY 2 cut(s) 584, 653
PagI TCATGA 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 625
PfeI GAWTC 2 cut(s) 797, 902
PkrI GCNGC 1 cut(s) 58
PmaCI CACGTG 1 cut(s) 441
PmlI CACGTG 1 cut(s) 441
Ppu21I YACGTR 2 cut(s) 441, 509
PshBI ATTAAT 1 cut(s) 948
PspCI CACGTG 1 cut(s) 441
PspEI GGTNACC 1 cut(s) 1081
PspN4I GGNNCC 1 cut(s) 1031
PspPI GGNCC 2 cut(s) 605, 1016
PstI CTGCAG 1 cut(s) 730
PsuI RGATCY 3 cut(s) 185, 273, 910
SapI GCTCTTC 1 cut(s) 625
SaqAI TTAA 3 cut(s) 363, 948, 1163
SatI GCNGC 1 cut(s) 57
Sau96I GGNCC 2 cut(s) 605, 1016
SduI GDGCHC 2 cut(s) 440, 537
SfaNI GCATC 3 cut(s) 111, 475, 644
SfcI CTRYAG 1 cut(s) 726
SinI GGWCC 2 cut(s) 605, 1016
SmlI CTYRAG 2 cut(s) 305, 661
SmoI CTYRAG 2 cut(s) 305, 661
Sse9I AATT 6 cut(s) 249, 403, 616, 884, 924, 1127
SsiI CCGC 1 cut(s) 843
SspI AATATT 2 cut(s) 298, 952
SspMI CTAG 3 cut(s) 423, 597, 939
TaaI ACNGT 2 cut(s) 226, 1039
TaiI ACGT 4 cut(s) 12, 443, 511, 859
TaqI TCGA 4 cut(s) 447, 859, 879, 957
TasI AATT 6 cut(s) 249, 403, 616, 884, 924, 1127
TfiI GAWTC 2 cut(s) 797, 902
Tru1I TTAA 3 cut(s) 363, 948, 1163
Tru9I TTAA 3 cut(s) 363, 948, 1163
TscAI CASTG 3 cut(s) 229, 660, 1042
TseFI GTSAC 3 cut(s) 66, 220, 1081
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 3 cut(s) 66, 220, 1081
TspDTI ATGAA 7 cut(s) 62, 354, 418, 810, 975, 1103, 1107
TspGWI ACGGA 1 cut(s) 628
TspRI CASTG 3 cut(s) 229, 660, 1042
VpaK11BI GGWCC 2 cut(s) 605, 1016
VspI ATTAAT 1 cut(s) 948
XapI RAATTY 1 cut(s) 924
XbaI TCTAGA 1 cut(s) 596
XceI RCATGY 2 cut(s) 584, 653
XmiI GTMKAC 1 cut(s) 732
XspI CTAG 3 cut(s) 423, 597, 939
ZraI GACGTC 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.