FvH4_5g37840

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014594
Physical Location & Seq
Forward (+)
27877262 .. 27883165
5904 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37840.t1

Sequence Viewer

Length: 2331 bp
ATGAAGCTCCCAGCTCTCATTCTCATCCCTCTTATCTTCTTCTCCACCCTCATCTTCCTCACCGAGTCAACTCAGCCTCCATATTCATGCGACTCGTCCAACCCTTCAACCGAGTCCTTCCTTTTCTGCAAAACCACCCTCCCCATCAACCAAAGAGTCCATGACCTCGTCTCCCGTCTCACATTGGACGAGAAGATCTCCCAACTCGTCAACTCGGCCCCTCCGATTCCCCGACTCGGCATCCCGAGTTACGAGTGGTGGTCGGAGGCTCTTCACGGCGTCGCTGACGTCGGCAAAGGCATCCGTCTCTACAGCACCATCAACAGCGCCACCAGCTTCCCTCAAGTCATATTAACCGCTGCTTCTTTCAATGAGCATCTCTGGTATCGGATCGGTCAGGTGATCGGAATCGAAGCGAGGGCAGTGTACAATGCAGGACAAGCGACGGGAATGACATTTTGGGCACCAAACATAAACATTTTCAGGGACCCAAGATGGGGGAGAGGGCAAGAGACTCCCGGAGAAGACCCATTGATGACGGCAAAATACTCGGTGGCGTATGTCCGGGGGGTTCAGGGTGATTCATATGAAGGAGGGAAGCTGAAGGTGGGTGGCCATCTCCAGGCCTCTGCTTGTTGTAAGCATTTCACGGCGTACGATCTGGACAACTGGAACAATGTGACTCGTTTTGGGTTTAATGCCAAGGTGACTCAGCAAGACCTAGCAGACACGTATCAGCCTCCATTCAAGAGTTGCGTCGAGCAAGGCAAAGCCAGTGGCATCATGTGTGCTTATAACCAAGTTAATGGAGTCCCAAGCTGTGCAGATCACAATCTCTTAACCAAGACTGCTCGAGGAGAATGGGGTTTTCATGGATATATCACGTCGGATTGTGACGCCGTGAGCATTATTTACGACGTGCAAGGATACGCTAAGCACCCGGAAGATGCCGTCGTCGACGTCCTGAAAGCAGGCATGGATGTGAACTGTGGAACCTACTTGCAAAATCACACAAAGAATGCAGTGCAGCAGAAAAAGCTGCCCGTATCATACATAGACAAAGCCCTCCACAACTTGTTCTCTATAAGGATGAGGTTAGGTCTGTTTGATGGCAACCCAACCAAGCTACCTTTCGGCAACATTGGTCCAGAGAAAGTGTGCTCTAAACAGCACCAAGCTTTAGCACTAGAAGCTGCTGAAGATGGCATCGTGCTTTTGAAGAACGCGGGCAAACTACTCCCACTACCAAAATCAAAGGGCATTTCACTTGCTGTAATCGGTCCCAATGCCAATGCTTCCGAAACACTTCTCGGAAACTATCACGGTCCACCGTGCAAGCTCATTACACCGTTACAAGGACTGCTAGGTTATGCTAAGAAAACAGTATACCACCCTGGATGTGATACAGTTAAATGCCCTAATCCTACGATTGATCAAGCTGTTAGAGTAGCACAGCAGGCAGATTATGTGGTTTTGATTGTGGGATTGGACCAAGGTGAAGAGAGAGAGGCGCACGATAGAGACCACTTAAACTTGCCTGGTAAGCAACAGCAACTCATTAGCAGTGTGGCTAAAGCCGCCAAGAAACCAGTGATTCTTGTGATTCTCTCTGGGGGTCCAGTTGACATATCTGCAGCGAAGTATAATCCAAAAATAGGAAGCATCCTGTGGGCTGGATATCCAGGTGAAGCTGGTGGATCTGCCCTGGCAGAAGTCATCTTTGGTGATCACAACCCAGGTGGAAGATTGCCAGTCACATGGTATACACAAGATTACATCAAAACACTAATGACAGACATGAGGATGAGGCCTGACAAAAGATCAGGCTATCCTGGGCGCACTTACAGATTCTACACAGGGAAAAGAGTGTTTGATTTCGGTTATGGCCTCAGCTACTCCAACTATGCTTACAATTTTGTATCTTCAGTGACCCAGAACAAGGTCTACCTGAATGAGTCATCGGTTGGTCTAGCAGCTAAGAATTCAGACTCCGGACGCTACCAGTTAGTTTCTGATCTAGGTGAAGAACTCTGTGAGAAAAAGTTGTTCAAGGTCACAGTTGGGGCTAAGAATGAGGGAGAGATGGCTGGTAAGCATCCAGTGTTGCTATTTGTTAGTCGGAAAAACCCGACAAATGGAAGTCCAATGAAACAGTTGGTTGGATTCAAGAGTGTGATACTAAGCGCTGGAGAAAAGGCTGAACTTGAATTCATGCTGAACCCTTGTGAGCACCTCAGCCATGCAAATGAAGATGGCTGGATGGTGGTGGAAGAAGGTTCGCGTTTCTTGGTTGTGGGTGATGTAGAGTACCCTATTGATATCATTGTTTGA

Protein Analysis

777

Amino Acids

84.99

Weight (kDa)

7.81

Isoelectric Point (pI)

28.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 105 - 359 4.2e-31 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 403 - 632 1.2e-54 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 701 - 767 2.9e-10 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014594)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78060
fragaria_vesca FvH4_5g37840
malus_domestica MD08G1179900.v1.1 MD15G1365200.v1.1
prunus_persica Prupe.1G513200_v2.0.a1
pyrus_communis pycom08g15400 pycom15g32750
rosa_chinensis RchiOBHm_Chr7g0239821
rosa_laevigata RLG00000000728
rosa_multiflora Rmu_sc0007193.1_g000008
rosa_roxburghii Rroxscaffold_3G00220790
rosa_rugosa Rorug07G0329500
rosa_samantha Rh7AG483700 Rh7BG456700 Rh7CG501200 Rh7DG469400
rosa_wichuraiana Rw7G040570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
AatII GACGTC 2 cut(s) 291, 963
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 4 cut(s) 957, 1386, 1763, 1944
AccII CGCG 2 cut(s) 1226, 2281
AccIII TCCGGA 1 cut(s) 1991
AciI CCGC 3 cut(s) 357, 1226, 1578
AclWI GGATC 2 cut(s) 398, 1705
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 2 cut(s) 1981, 2207
AcuI CTGAAG 3 cut(s) 623, 1218, 1908
AcyI GRCGYC 4 cut(s) 279, 288, 897, 960
AfaI GTAC 3 cut(s) 428, 656, 2309
AfeI AGCGCT 1 cut(s) 2185
AfiI CCNNNNNNNGG 8 cut(s) 236, 496, 497, 622, 1355, 1655, 1939, 2135
AflIII ACRYGT 1 cut(s) 729
AgsI TTSAA 7 cut(s) 108, 370, 748, 1219, 2050, 2167, 2207
AjiI CACGTC 2 cut(s) 885, 919
AjnI CCWGG 7 cut(s) 621, 1393, 1537, 1681, 1704, 1735, 1831
AjuI GAANNNNNNNTTGG 6 cut(s) 442, 474, 1115, 1147, 1704, 1736
Alw21I GWGCWC 2 cut(s) 1163, 2232
Alw26I GTCTC 5 cut(s) 175, 182, 311, 506, 1515
AlwI GGATC 2 cut(s) 398, 1705
Ama87I CYCGRG 2 cut(s) 244, 852
Aor13HI TCCGGA 1 cut(s) 1991
Aor51HI AGCGCT 1 cut(s) 2185
AoxI GGCC 5 cut(s) 216, 613, 624, 1808, 1885
ApeKI GCWGC 6 cut(s) 359, 1027, 1039, 1193, 1634, 1973
ApoI RAATTY 2 cut(s) 1981, 2207
Asp700I GAANNNNTTC 1 cut(s) 1305
AspLEI GCGC 4 cut(s) 329, 1513, 1839, 2186
AspS9I GGNCC 7 cut(s) 217, 487, 1145, 1280, 1325, 1489, 1616
AsuC2I CCSGG 3 cut(s) 519, 566, 941
AsuHPI GGTGA 9 cut(s) 52, 412, 590, 718, 1508, 1697, 1736, 2033, 2309
AvaI CYCGRG 2 cut(s) 244, 852
AvaII GGWCC 6 cut(s) 487, 1145, 1280, 1325, 1489, 1616
BaeGI GKGCMC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 615
BanI GGYRCC 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 2 cut(s) 1163, 2232
BbvCI CCTCAGC 2 cut(s) 1889, 2234
BbvI GCAGC 6 cut(s) 346, 1026, 1039, 1180, 1646, 1985
BccI CCATC 9 cut(s) 152, 326, 489, 624, 1103, 1196, 2077, 2246, 2254
BceAI ACGGC 5 cut(s) 292, 555, 666, 884, 935
BcgI CGANNNNNNTGC 2 cut(s) 531, 565
BciT130I CCWGG 7 cut(s) 623, 1395, 1539, 1683, 1706, 1737, 1833
BciVI GTATCC 1 cut(s) 920
BclI TGATCA 2 cut(s) 1432, 1726
BcnI CCSGG 3 cut(s) 519, 566, 941
BcoDI GTCTC 5 cut(s) 175, 182, 311, 506, 1515
BfaI CTAG 5 cut(s) 722, 1187, 1364, 1970, 2018
BfmI CTRYAG 2 cut(s) 310, 1632
BfoI RGCGCY 2 cut(s) 330, 2187
BfuI GTATCC 1 cut(s) 920
BglII AGATCT 1 cut(s) 195
BisI GCNGC 7 cut(s) 360, 1028, 1040, 1194, 1578, 1635, 1974
BlpI GCTNAGC 1 cut(s) 933
BlsI GCNGC 7 cut(s) 361, 1029, 1041, 1195, 1579, 1636, 1975
Bme18I GGWCC 6 cut(s) 487, 1145, 1280, 1325, 1489, 1616
BmeT110I CYCGRG 2 cut(s) 244, 852
BmgBI CACGTC 2 cut(s) 885, 919
BmgT120I GGNCC 7 cut(s) 217, 487, 1145, 1280, 1325, 1489, 1616
BmiI GGNNCC 7 cut(s) 219, 465, 488, 489, 994, 1282, 1617
BmsI GCATC 8 cut(s) 249, 309, 385, 789, 937, 1215, 1671, 2104
BpiI GAAGAC 1 cut(s) 531
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 2 cut(s) 605, 2208
Bpu10I CCTNAGC 2 cut(s) 1889, 2234
Bpu1102I GCTNAGC 1 cut(s) 933
BpuEI CTTGAG 1 cut(s) 327
BpuMI CCSGG 3 cut(s) 519, 566, 941
BsaAI YACGTR 1 cut(s) 732
BsaBI GATNNNNATC 2 cut(s) 407, 831
BsaHI GRCGYC 4 cut(s) 279, 288, 897, 960
BsaI GGTCTC 1 cut(s) 1515
BsaJI CCNNGG 7 cut(s) 565, 702, 1393, 1492, 1704, 1735, 1832
BsaWI WCCGGW 1 cut(s) 1991
BsaXI ACNNNNNCTCC 4 cut(s) 61, 91, 155, 185
Bsc4I CCNNNNNNNGG 8 cut(s) 236, 496, 497, 622, 1355, 1655, 1939, 2135
Bse1I ACTGG 7 cut(s) 674, 774, 1589, 1619, 1751, 2002, 2099
Bse8I GATNNNNATC 2 cut(s) 407, 831
BseAI TCCGGA 1 cut(s) 1991
BseBI CCWGG 7 cut(s) 623, 1395, 1539, 1683, 1706, 1737, 1833
BseDI CCNNGG 7 cut(s) 565, 702, 1393, 1492, 1704, 1735, 1832
BseJI GATNNNNATC 2 cut(s) 407, 831
BseLI CCNNNNNNNGG 8 cut(s) 236, 496, 497, 622, 1355, 1655, 1939, 2135
BseMII CTCAG 4 cut(s) 86, 725, 1903, 2248
BseNI ACTGG 7 cut(s) 674, 774, 1589, 1619, 1751, 2002, 2099
BseRI GAGGAG 1 cut(s) 870
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 6 cut(s) 346, 1026, 1039, 1180, 1646, 1985
BseYI CCCAGC 1 cut(s) 10
BsgI GTGCAG 2 cut(s) 843, 1046
Bsh1236I CGCG 2 cut(s) 1226, 2281
BshFI GGCC 5 cut(s) 218, 615, 626, 1810, 1887
BshNI GGYRCC 1 cut(s) 463
BsiHKAI GWGCWC 2 cut(s) 1163, 2232
BsiHKCI CYCGRG 2 cut(s) 244, 852
BsiSI CCGG 4 cut(s) 519, 565, 941, 1992
BsiWI CGTACG 1 cut(s) 654
BslFI GGGAC 3 cut(s) 500, 797, 1266
BslI CCNNNNNNNGG 8 cut(s) 236, 496, 497, 622, 1355, 1655, 1939, 2135
BsmAI GTCTC 5 cut(s) 175, 182, 311, 506, 1515
BsmBI CGTCTC 3 cut(s) 175, 182, 311
BsmFI GGGAC 3 cut(s) 500, 797, 1266
BsmI GAATGC 1 cut(s) 1024
BsnI GGCC 5 cut(s) 218, 615, 626, 1810, 1887
Bso31I GGTCTC 1 cut(s) 1515
BsoBI CYCGRG 2 cut(s) 244, 852
Bsp1286I GDGCHC 3 cut(s) 466, 1163, 2232
Bsp13I TCCGGA 1 cut(s) 1991
Bsp1407I TGTACA 1 cut(s) 426
Bsp1720I GCTNAGC 1 cut(s) 933
BspACI CCGC 3 cut(s) 357, 1226, 1578
BspANI GGCC 5 cut(s) 218, 615, 626, 1810, 1887
BspCNI CTCAG 4 cut(s) 85, 724, 1902, 2247
BspEI TCCGGA 1 cut(s) 1991
BspFNI CGCG 2 cut(s) 1226, 2281
BspLI GGNNCC 7 cut(s) 219, 465, 488, 489, 994, 1282, 1617
BspMAI CTGCAG 1 cut(s) 1636
BspPI GGATC 2 cut(s) 398, 1705
BspQI GCTCTTC 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 463
BspTNI GGTCTC 1 cut(s) 1515
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 7 cut(s) 674, 774, 1589, 1619, 1751, 2002, 2099
BssECI CCNNGG 7 cut(s) 565, 702, 1393, 1492, 1704, 1735, 1832
BssNAI GTATAC 2 cut(s) 1387, 1764
BssNI GRCGYC 4 cut(s) 279, 288, 897, 960
BssT1I CCWWGG 2 cut(s) 702, 1492
Bst1107I GTATAC 2 cut(s) 1387, 1764
Bst2UI CCWGG 7 cut(s) 623, 1395, 1539, 1683, 1706, 1737, 1833
Bst4CI ACNGT 8 cut(s) 989, 1325, 1332, 1350, 1384, 1408, 2059, 2154
Bst6I CTCTTC 2 cut(s) 276, 1494
BstACI GRCGYC 4 cut(s) 279, 288, 897, 960
BstAUI TGTACA 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 732
BstC8I GCNNGC 4 cut(s) 973, 1228, 1337, 1458
BstDEI CTNAG 9 cut(s) 72, 711, 933, 1374, 1889, 1977, 2067, 2180, 2234
BstFNI CGCG 2 cut(s) 1226, 2281
BstH2I RGCGCY 2 cut(s) 330, 2187
BstHHI GCGC 4 cut(s) 329, 1513, 1839, 2186
BstMAI GTCTC 5 cut(s) 175, 182, 311, 506, 1515
BstMWI GCNNNNNNNGC 7 cut(s) 333, 440, 1036, 1190, 1457, 1543, 1577
BstNI CCWGG 7 cut(s) 623, 1395, 1539, 1683, 1706, 1737, 1833
BstSFI CTRYAG 2 cut(s) 310, 1632
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 2 cut(s) 1226, 2281
BstV1I GCAGC 6 cut(s) 346, 1026, 1039, 1180, 1646, 1985
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 2 cut(s) 195, 1697
BstXI CCANNNNNNTGG 2 cut(s) 806, 1758
BstYI RGATCY 2 cut(s) 195, 1697
BstZ17I GTATAC 2 cut(s) 1387, 1764
BsuI GTATCC 1 cut(s) 920
BsuRI GGCC 5 cut(s) 218, 615, 626, 1810, 1887
BtrI CACGTC 2 cut(s) 885, 919
BtsI GCAGTG 3 cut(s) 429, 1029, 1570
BtsIMutI CAGTG 7 cut(s) 429, 781, 1029, 1570, 1596, 1932, 2106
Cac8I GCNNGC 4 cut(s) 973, 1228, 1337, 1458
CfoI GCGC 4 cut(s) 329, 1513, 1839, 2186
Cfr13I GGNCC 7 cut(s) 217, 487, 1145, 1280, 1325, 1489, 1616
CseI GACGC 4 cut(s) 268, 745, 905, 2004
Csp6I GTAC 3 cut(s) 427, 655, 2308
CspCI CAANNNNNGTGG 4 cut(s) 757, 792, 1720, 1755
CviAII CATG 9 cut(s) 87, 161, 784, 872, 976, 1758, 1798, 2212, 2240
CviQI GTAC 3 cut(s) 427, 655, 2308
DdeI CTNAG 9 cut(s) 72, 711, 933, 1374, 1889, 1977, 2067, 2180, 2234
EaeI YGGCCR 1 cut(s) 613
Eam1104I CTCTTC 2 cut(s) 276, 1494
EarI CTCTTC 2 cut(s) 276, 1494
Eco130I CCWWGG 2 cut(s) 702, 1492
Eco147I AGGCCT 2 cut(s) 626, 1810
Eco31I GGTCTC 1 cut(s) 1515
Eco32I GATATC 2 cut(s) 1679, 2320
Eco47I GGWCC 6 cut(s) 487, 1145, 1280, 1325, 1489, 1616
Eco47III AGCGCT 1 cut(s) 2185
Eco57I CTGAAG 3 cut(s) 623, 1218, 1908
Eco88I CYCGRG 2 cut(s) 244, 852
EcoO109I RGGNCCY 1 cut(s) 487
EcoRI GAATTC 2 cut(s) 1981, 2207
EcoRII CCWGG 7 cut(s) 621, 1393, 1537, 1681, 1704, 1735, 1831
EcoRV GATATC 2 cut(s) 1679, 2320
EcoT14I CCWWGG 2 cut(s) 702, 1492
ErhI CCWWGG 2 cut(s) 702, 1492
Esp3I CGTCTC 3 cut(s) 175, 182, 311
FaeI CATG 9 cut(s) 90, 164, 787, 875, 979, 1761, 1801, 2215, 2243
FaqI GGGAC 3 cut(s) 500, 797, 1266
FatI CATG 9 cut(s) 86, 160, 783, 871, 975, 1757, 1797, 2211, 2239
FauI CCCGC 1 cut(s) 1219
FauNDI CATATG 1 cut(s) 586
FbaI TGATCA 2 cut(s) 1432, 1726
FblI GTMKAC 4 cut(s) 957, 1386, 1763, 1944
Fnu4HI GCNGC 7 cut(s) 360, 1028, 1040, 1194, 1578, 1635, 1974
Fsp4HI GCNGC 7 cut(s) 360, 1028, 1040, 1194, 1578, 1635, 1974
FspBI CTAG 5 cut(s) 722, 1187, 1364, 1970, 2018
GlaI GCGC 4 cut(s) 328, 1512, 1838, 2185
GluI GCNGC 7 cut(s) 360, 1028, 1040, 1194, 1578, 1635, 1974
GsaI CCCAGC 1 cut(s) 14
GsuI CTGGAG 2 cut(s) 605, 2208
HaeII RGCGCY 2 cut(s) 330, 2187
HaeIII GGCC 5 cut(s) 218, 615, 626, 1810, 1887
HapII CCGG 4 cut(s) 519, 565, 941, 1992
HgaI GACGC 4 cut(s) 268, 745, 905, 2004
HhaI GCGC 4 cut(s) 329, 1513, 1839, 2186
Hin1I GRCGYC 4 cut(s) 279, 288, 897, 960
Hin1II CATG 9 cut(s) 90, 164, 787, 875, 979, 1761, 1801, 2215, 2243
Hin6I GCGC 4 cut(s) 327, 1511, 1837, 2184
HinP1I GCGC 4 cut(s) 327, 1511, 1837, 2184
HincII GTYRAC 4 cut(s) 69, 211, 958, 1624
HindII GTYRAC 4 cut(s) 69, 211, 958, 1624
HindIII AAGCTT 1 cut(s) 1176
HpaII CCGG 4 cut(s) 519, 565, 941, 1992
HphI GGTGA 9 cut(s) 52, 412, 590, 718, 1508, 1697, 1736, 2033, 2309
Hpy188III TCNNGA 7 cut(s) 244, 662, 748, 964, 1148, 1992, 2167
Hpy99I CGWCG 9 cut(s) 284, 293, 448, 761, 889, 920, 956, 959, 962
HpyAV CCTTC 5 cut(s) 114, 127, 584, 598, 2267
HpyCH4III ACNGT 8 cut(s) 989, 1325, 1332, 1350, 1384, 1408, 2059, 2154
HpyCH4IV ACGT 5 cut(s) 288, 731, 884, 918, 960
HpyF10VI GCNNNNNNNGC 7 cut(s) 333, 440, 1036, 1190, 1457, 1543, 1577
HpyF3I CTNAG 9 cut(s) 72, 711, 933, 1374, 1889, 1977, 2067, 2180, 2234
HpySE526I ACGT 5 cut(s) 288, 731, 884, 918, 960
Hsp92I GRCGYC 4 cut(s) 279, 288, 897, 960
Hsp92II CATG 9 cut(s) 90, 164, 787, 875, 979, 1761, 1801, 2215, 2243
HspAI GCGC 4 cut(s) 327, 1511, 1837, 2184
KflI GGGWCCC 1 cut(s) 487
Kpn2I TCCGGA 1 cut(s) 1991
Ksp22I TGATCA 2 cut(s) 1432, 1726
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 1 cut(s) 12
Lsp1109I GCAGC 6 cut(s) 346, 1026, 1039, 1180, 1646, 1985
LweI GCATC 8 cut(s) 249, 309, 385, 789, 937, 1215, 1671, 2104
MaeI CTAG 5 cut(s) 722, 1187, 1364, 1970, 2018
MaeII ACGT 5 cut(s) 288, 731, 884, 918, 960
MaeIII GTNAC 8 cut(s) 248, 679, 706, 893, 1350, 1753, 1927, 2053
MflI RGATCY 2 cut(s) 195, 1697
MhlI GDGCHC 3 cut(s) 466, 1163, 2232
MlsI TGGCCA 1 cut(s) 615
MluCI AATT 3 cut(s) 1912, 1981, 2207
MluNI TGGCCA 1 cut(s) 615
MmeI TCCRAC 6 cut(s) 123, 243, 867, 1923, 2099, 2140
Mox20I TGGCCA 1 cut(s) 615
MroI TCCGGA 1 cut(s) 1991
MroXI GAANNNNTTC 1 cut(s) 1305
MscI TGGCCA 1 cut(s) 615
MseI TTAA 6 cut(s) 353, 696, 804, 839, 1410, 1529
MslI CAYNNNNRTG 4 cut(s) 85, 980, 1802, 2244
Msp20I TGGCCA 1 cut(s) 615
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 4 cut(s) 519, 565, 941, 1992
Mva1269I GAATGC 1 cut(s) 1024
MvaI CCWGG 7 cut(s) 623, 1395, 1539, 1683, 1706, 1737, 1833
MvnI CGCG 2 cut(s) 1226, 2281
MwoI GCNNNNNNNGC 7 cut(s) 333, 440, 1036, 1190, 1457, 1543, 1577
NciI CCSGG 3 cut(s) 519, 566, 941
NdeI CATATG 1 cut(s) 586
NlaIII CATG 9 cut(s) 90, 164, 787, 875, 979, 1761, 1801, 2215, 2243
NlaIV GGNNCC 7 cut(s) 219, 465, 488, 489, 994, 1282, 1617
NmeAIII GCCGAG 2 cut(s) 194, 216
NmuCI GTSAC 6 cut(s) 679, 706, 893, 1753, 1927, 2053
PaeR7I CTCGAG 1 cut(s) 852
PceI AGGCCT 2 cut(s) 626, 1810
PciSI GCTCTTC 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PctI GAATGC 1 cut(s) 1024
PdmI GAANNNNTTC 1 cut(s) 1305
PfeI GAWTC 7 cut(s) 226, 408, 581, 1594, 1603, 1848, 2163
Pfl23II CGTACG 1 cut(s) 654
PflFI GACNNNGTC 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 7 cut(s) 361, 1029, 1041, 1195, 1579, 1636, 1975
Ppu21I YACGTR 1 cut(s) 732
PpuMI RGGWCCY 1 cut(s) 487
PsiI TTATAA 1 cut(s) 795
Psp5II RGGWCCY 1 cut(s) 487
Psp6I CCWGG 7 cut(s) 621, 1393, 1537, 1681, 1704, 1735, 1831
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 7 cut(s) 621, 1393, 1537, 1681, 1704, 1735, 1831
PspLI CGTACG 1 cut(s) 654
PspN4I GGNNCC 7 cut(s) 219, 465, 488, 489, 994, 1282, 1617
PspPI GGNCC 7 cut(s) 217, 487, 1145, 1280, 1325, 1489, 1616
PspPPI RGGWCCY 1 cut(s) 487
PspXI VCTCGAGB 1 cut(s) 852
PsrI GAACNNNNNNTAC 2 cut(s) 1928, 1960
PstI CTGCAG 1 cut(s) 1636
PsuI RGATCY 2 cut(s) 195, 1697
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 3 cut(s) 428, 656, 2309
RsaNI GTAC 3 cut(s) 427, 655, 2308
RseI CAYNNNNRTG 4 cut(s) 85, 980, 1802, 2244
SalI GTCGAC 1 cut(s) 956
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 6 cut(s) 353, 696, 804, 839, 1410, 1529
SatI GCNGC 7 cut(s) 360, 1028, 1040, 1194, 1578, 1635, 1974
Sau96I GGNCC 7 cut(s) 217, 487, 1145, 1280, 1325, 1489, 1616
SduI GDGCHC 3 cut(s) 466, 1163, 2232
SfaNI GCATC 8 cut(s) 249, 309, 385, 789, 937, 1215, 1671, 2104
SfcI CTRYAG 2 cut(s) 310, 1632
Sfr274I CTCGAG 1 cut(s) 852
SgrDI CGTCGACG 1 cut(s) 956
SinI GGWCC 6 cut(s) 487, 1145, 1280, 1325, 1489, 1616
SlaI CTCGAG 1 cut(s) 852
SmiMI CAYNNNNRTG 4 cut(s) 85, 980, 1802, 2244
SmlI CTYRAG 2 cut(s) 342, 852
SmoI CTYRAG 2 cut(s) 342, 852
Sse9I AATT 3 cut(s) 1912, 1981, 2207
SseBI AGGCCT 2 cut(s) 626, 1810
SsiI CCGC 3 cut(s) 357, 1226, 1578
SspMI CTAG 5 cut(s) 722, 1187, 1364, 1970, 2018
StuI AGGCCT 2 cut(s) 626, 1810
StyI CCWWGG 2 cut(s) 702, 1492
TaaI ACNGT 8 cut(s) 989, 1325, 1332, 1350, 1384, 1408, 2059, 2154
TaiI ACGT 5 cut(s) 291, 734, 887, 921, 963
TaqI TCGA 4 cut(s) 411, 759, 853, 957
TaqII GACCGA 2 cut(s) 383, 1268
TasI AATT 3 cut(s) 1912, 1981, 2207
TatI WGTACW 1 cut(s) 426
TauI GCSGC 1 cut(s) 1580
TfiI GAWTC 7 cut(s) 226, 408, 581, 1594, 1603, 1848, 2163
Tru1I TTAA 6 cut(s) 353, 696, 804, 839, 1410, 1529
Tru9I TTAA 6 cut(s) 353, 696, 804, 839, 1410, 1529
TscAI CASTG 7 cut(s) 429, 781, 1029, 1570, 1596, 1932, 2106
TseFI GTSAC 6 cut(s) 679, 706, 893, 1753, 1927, 2053
TseI GCWGC 6 cut(s) 359, 1027, 1039, 1193, 1634, 1973
Tsp45I GTSAC 6 cut(s) 679, 706, 893, 1753, 1927, 2053
TspDTI ATGAA 8 cut(s) 17, 75, 573, 603, 860, 2162, 2200, 2262
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 7 cut(s) 429, 781, 1029, 1570, 1596, 1932, 2106
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 6 cut(s) 487, 1145, 1280, 1325, 1489, 1616
XapI RAATTY 2 cut(s) 1981, 2207
XhoI CTCGAG 1 cut(s) 852
XmiI GTMKAC 4 cut(s) 957, 1386, 1763, 1944
XmnI GAANNNNTTC 1 cut(s) 1305
XspI CTAG 5 cut(s) 722, 1187, 1364, 1970, 2018
ZraI GACGTC 2 cut(s) 289, 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.