Prupe.1G513200_v2.0.a1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014594
Physical Location & Seq
Reverse (-)
42231732 .. 42236156
4425 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G513200.1

Sequence Viewer

Length: 2346 bp
ATGAAGCTTCAAGCTCTCACTCCTCCCACACCTCTTTTCTGCTTCTTCTTCTTCACCACCCTCCTCTTTTACCCTACCGAGTCAACGCAGCCTCCATATGCCTGTGACTCGTCCCAGCCATCAACCAGCTCCTACCCTTTCTGCAAAACCACCCTCCCCATCAACCAAAGAGTCCAGGACCTGGTCTCCCGCCTCACATTGGATGAGAAAATCTCCCAACTAGTTAACTCGGCCCCTCCGATTCCCCGACTCAGCATCCCGAGTTACGAGTGGTGGTCCGAGGCATTACACGGCGTCGCCGACGTCGGCAAAGGAATCAATCTTTACGGCACCATTTCTAACGCCACCAGCTTCCCTCAAGTCATCCTCACCGCCGCCTCCTTCAACGAACACCTCTGGTACCGCATTGGGCAGGTGATTGGCACTGAAGCTAGGGCATTGTACAATGCTGGGCAAGCAACAGGGATGACATTTTGGGCACCAAACATTAACATTTTCAGGGACCCAAGGTGGGGGAGAGGGCAGGAGACACCTGGCGAAGACCCATTGGTGGTAGGCAAATACGCCGTGTCGTATGTGAGAGGCGTTCAAGGTGATTCTTTTGAAGGAGGGAAGCTCAAGGTCGGTGGTCGTCTTCAAGCTTCTGCTTGCTGCAAGCATTTCACAGCTTATGATTTGGACAACTGGAAAAGCGTCACGCGCTTTGGCTTCGATGCTCGCGTGAGTGAACAAGACCTAGCAGACACATACCAGCCTCCATTCAAGAGCTGCGTGCAGCAAGGCCAAGCCAGTGGCATAATGTGTGCTTATAATCGTGTCAATGGGGTTCCAAGCTGCGCAGATTACAATCTCCTAACCAAGGTTGCTAGAGGCCAATGGGATTTTCATGGGTACATCACGTCGGACTGCGATGCGGTGTCCATCATTCGCGATGTGCAGGGGTACGCTAAAACGCCCGAAGATGCAGTCGGTGATGTGCTCAAAGCAGGCATGGACGTGAACTGTGGATCCTACTTGAAGGATCACACTAAATCTGCAGTTCAGCAGAAAAAGCTGGATGTGTCAGAAATAGACAGAGCCCTCCACAACCTCTTCTCTATAAGGATGAGGTTAGGCCTGTTTGACGGCAGTCCACTCGAGCAACCTTATGGCAACATTGGTCCAGACCAAGCGTGCTCCAAGGAGCACCAGGCTTTAGCCCTTGAAGCTGCTCAAGATGGCATAGTGCTTTTGAAAAACTCTGGCAGACTTCTCCCATTGCCTAAATCAAAAGCCATTTCTCTTGCTGTCATTGGTCCCAATGCCAATGCTTCAGAAACACTTCTTGGAAATTATCATGGCCGTCCATGTAAATCCATAACCCCCTTAAAAGCATTGCAGGGTTATGCTAAGTACACAAATTATGAAGCAGGCTGTGACACAGTTAAGTGTCCCCAAGCTACAATTGATAAAGCAGTTGAAGCAGCAAAAGCAGCAGATTATGTGGTTTTGATTATGGGGCTGGACCAAAGTCAAGAGAGAGAGGCGCATGACAGAAGGCACTTGGGCCTGCCTGGAAAGCAACAGGAACTCATTTCCAGTGTGGCTAAAGCTGCTAAAAAACCTGTTATTCTTGTGATTCTTAGTGGGGGTCCAGTTGACATAACTCCAGCCAAGTATGACAAAAAAATTGGAGGCATCTTGTGGGCTGGATATCCTGGTGAAGCCGGAGGAATCGCCCTTGCTGAAATCATCTTTGGTGATCACAATCCAGGTGGAAGACTGCCAGTGACTTGGTACACACAAGATTATGTCAAGGTACCAATGACAGACATGAGGATGAGGCCTGACACAAAAACAGGTTATCCTGGGCGCACTTACAGATTCTACAAAGGCGGAAATGTTTATCACTTTGGTTTTGGCCTCAGCTACTCCAACTACATTTATGAGTTTGCCTCCGCAATAGCTCAAAACAAGCTATACTTAAATGAATCATCCATTAGTCCCGAAGTTGAGTCCTCAGACTCCGGCCATTTCCGGTTAATCCCAGATTTAAGTGAAGAATTCTGTGAGAAAAAGAAGTTCCCAGTCAGAGTTGCGGTCAAAAATCATGGGGAGATGGTTGGTAAGCACCCTGTGTTGCTTTTTGTGGGTCAGAAGAATCCCAACAATGGAAGCCCAATGAAACAGTTGGTTGGATTTCAGAGTGTGATATTAAGTGCTGGGGAAAGAGCTGAACTTGAATTCATTTTGAACCCTTGTGAGCACCTCAGCCATGCAAATGAGGGTGGTTTGATGGTAGTGGAAGAAGGTTCTTATTTCTTGCAAGTAGGAGATGTAGAGTACCCTCTTGATATCATAGTTTGA

Protein Analysis

782

Amino Acids

85.67

Weight (kDa)

6.51

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014594)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78060
fragaria_vesca FvH4_5g37840
malus_domestica MD08G1179900.v1.1 MD15G1365200.v1.1
prunus_persica Prupe.1G513200_v2.0.a1
pyrus_communis pycom08g15400 pycom15g32750
rosa_chinensis RchiOBHm_Chr7g0239821
rosa_laevigata RLG00000000728
rosa_multiflora Rmu_sc0007193.1_g000008
rosa_roxburghii Rroxscaffold_3G00220790
rosa_rugosa Rorug07G0329500
rosa_samantha Rh7AG483700 Rh7BG456700 Rh7CG501200 Rh7DG469400
rosa_wichuraiana Rw7G040570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 810
AarI CACCTGC 1 cut(s) 403
AatII GACGTC 1 cut(s) 306
Acc16I TGCGCA 1 cut(s) 838
Acc36I ACCTGC 1 cut(s) 403
Acc65I GGTACC 2 cut(s) 399, 1798
AccB1I GGYRCC 4 cut(s) 329, 399, 478, 1798
AccB7I CCANNNNNTGG 1 cut(s) 181
AccII CGCG 3 cut(s) 700, 720, 930
AciI CCGC 8 cut(s) 190, 372, 375, 403, 914, 1875, 1938, 2078
AclWI GGATC 3 cut(s) 1002, 1015, 1029
AcoI YGGCCR 2 cut(s) 1339, 2008
AcsI RAATTY 2 cut(s) 2042, 2222
AcuI CTGAAG 2 cut(s) 447, 1296
AcyI GRCGYC 2 cut(s) 294, 303
AdeI CACNNNGTG 1 cut(s) 2116
AfaI GTAC 8 cut(s) 401, 443, 893, 944, 1394, 1778, 1800, 2324
AfiI CCNNNNNNNGG 7 cut(s) 181, 199, 511, 512, 550, 859, 2150
AhlI ACTAGT 1 cut(s) 220
AjiI CACGTC 2 cut(s) 900, 997
AjnI CCWGG 8 cut(s) 174, 180, 532, 1188, 1552, 1696, 1750, 1846
AjuI GAANNNNNNNTTGG 6 cut(s) 867, 899, 1308, 1340, 1719, 1751
AloI GAACNNNNNNTCC 2 cut(s) 992, 1024
Alw21I GWGCWC 4 cut(s) 981, 1178, 1188, 2247
Alw26I GTCTC 2 cut(s) 190, 521
AlwI GGATC 3 cut(s) 1002, 1015, 1029
AlwNI CAGNNNCTG 1 cut(s) 181
Ama87I CYCGRG 2 cut(s) 259, 1136
AoxI GGCC 9 cut(s) 231, 781, 871, 1114, 1339, 1546, 1823, 1900, 2008
ApeKI GCWGC 9 cut(s) 88, 651, 768, 775, 834, 1208, 1463, 1472, 1592
ApoI RAATTY 2 cut(s) 2042, 2222
ArsI GACNNNNNNTTYG 2 cut(s) 951, 983
Asp700I GAANNNNTTC 1 cut(s) 1320
Asp718I GGTACC 2 cut(s) 399, 1798
AspLEI GCGC 4 cut(s) 702, 839, 1528, 1854
AspS9I GGNCC 9 cut(s) 178, 232, 276, 502, 1160, 1295, 1504, 1546, 1631
AsuHPI GGTGA 7 cut(s) 46, 361, 427, 605, 983, 1712, 1751
AvaI CYCGRG 2 cut(s) 259, 1136
AvaII GGWCC 7 cut(s) 178, 276, 502, 1160, 1295, 1504, 1631
BaeGI GKGCMC 1 cut(s) 481
BamHI GGATCC 1 cut(s) 1007
BanI GGYRCC 4 cut(s) 329, 399, 478, 1798
BanII GRGCYC 1 cut(s) 1081
BbsI GAAGAC 3 cut(s) 546, 626, 1765
Bbv12I GWGCWC 4 cut(s) 981, 1178, 1188, 2247
BbvCI CCTCAGC 2 cut(s) 1904, 2249
BbvI GCAGC 9 cut(s) 100, 638, 755, 787, 821, 1195, 1475, 1484, 1579
BccI CCATC 6 cut(s) 127, 167, 929, 1211, 2092, 2269
BceAI ACGGC 5 cut(s) 307, 343, 551, 1141, 1326
BcgI CGANNNNNNTGC 2 cut(s) 1117, 1151
BciT130I CCWGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
BclI TGATCA 1 cut(s) 1741
BcoDI GTCTC 2 cut(s) 190, 521
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 4 cut(s) 221, 432, 737, 867
BfmI CTRYAG 1 cut(s) 1035
BfuAI ACCTGC 1 cut(s) 403
Bme1390I CCNGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
Bme18I GGWCC 7 cut(s) 178, 276, 502, 1160, 1295, 1504, 1631
BmeT110I CYCGRG 2 cut(s) 259, 1136
BmgBI CACGTC 2 cut(s) 900, 997
BmgT120I GGNCC 9 cut(s) 178, 232, 276, 502, 1160, 1295, 1504, 1546, 1631
BmrFI CCNGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
BmrI ACTGGG 1 cut(s) 2060
BmsI GCATC 5 cut(s) 264, 703, 901, 952, 1686
BmuI ACTGGG 1 cut(s) 2060
BoxI GACNNNNGTC 2 cut(s) 1128, 1509
BpiI GAAGAC 3 cut(s) 546, 626, 1765
BplI GAGNNNNNCTC 6 cut(s) 197, 229, 600, 632, 1919, 1951
BpmI CTGGAG 1 cut(s) 1632
Bpu10I CCTNAGC 2 cut(s) 1904, 2249
BpuEI CTTGAG 3 cut(s) 342, 602, 1197
BsaBI GATNNNNATC 2 cut(s) 846, 1746
BsaHI GRCGYC 2 cut(s) 294, 303
BsaI GGTCTC 1 cut(s) 190
BsaJI CCNNGG 5 cut(s) 279, 506, 858, 1179, 1847
BsaWI WCCGGW 1 cut(s) 2016
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 170, 200
Bsc4I CCNNNNNNNGG 7 cut(s) 181, 199, 511, 512, 550, 859, 2150
Bse1I ACTGG 6 cut(s) 689, 789, 1578, 1634, 1766, 2066
Bse3DI GCAATG 2 cut(s) 1256, 1373
Bse8I GATNNNNATC 2 cut(s) 846, 1746
BseBI CCWGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
BseDI CCNNGG 5 cut(s) 279, 506, 858, 1179, 1847
BseGI GGATG 8 cut(s) 208, 255, 363, 471, 1063, 1110, 1824, 1973
BseJI GATNNNNATC 2 cut(s) 846, 1746
BseLI CCNNNNNNNGG 7 cut(s) 181, 199, 511, 512, 550, 859, 2150
BseMI GCAATG 2 cut(s) 1256, 1373
BseMII CTCAG 4 cut(s) 265, 1918, 2013, 2263
BseNI ACTGG 6 cut(s) 689, 789, 1578, 1634, 1766, 2066
BseRI GAGGAG 2 cut(s) 12, 53
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 9 cut(s) 100, 638, 755, 787, 821, 1195, 1475, 1484, 1579
BseYI CCCAGC 3 cut(s) 114, 449, 2201
BsgI GTGCAG 2 cut(s) 794, 956
Bsh1236I CGCG 3 cut(s) 700, 720, 930
BshFI GGCC 9 cut(s) 233, 783, 873, 1116, 1341, 1548, 1825, 1902, 2010
BshNI GGYRCC 4 cut(s) 329, 399, 478, 1798
BsiHKAI GWGCWC 4 cut(s) 981, 1178, 1188, 2247
BsiHKCI CYCGRG 2 cut(s) 259, 1136
BsiSI CCGG 3 cut(s) 1707, 2007, 2017
BslFI GGGAC 5 cut(s) 97, 515, 1281, 1416, 1968
BslI CCNNNNNNNGG 7 cut(s) 181, 199, 511, 512, 550, 859, 2150
BsmAI GTCTC 2 cut(s) 190, 521
BsmFI GGGAC 5 cut(s) 97, 515, 1281, 1416, 1968
BsnI GGCC 9 cut(s) 233, 783, 873, 1116, 1341, 1548, 1825, 1902, 2010
Bso31I GGTCTC 1 cut(s) 190
BsoBI CYCGRG 2 cut(s) 259, 1136
Bsp1286I GDGCHC 6 cut(s) 481, 981, 1081, 1178, 1188, 2247
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 3 cut(s) 1007, 1021, 1741
Bsp68I TCGCGA 1 cut(s) 930
BspACI CCGC 8 cut(s) 190, 372, 375, 403, 914, 1875, 1938, 2078
BspANI GGCC 9 cut(s) 233, 783, 873, 1116, 1341, 1548, 1825, 1902, 2010
BspCNI CTCAG 4 cut(s) 264, 1917, 2012, 2262
BspFNI CGCG 3 cut(s) 700, 720, 930
BspMAI CTGCAG 1 cut(s) 1039
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 3 cut(s) 1002, 1015, 1029
BspT107I GGYRCC 4 cut(s) 329, 399, 478, 1798
BspTNI GGTCTC 1 cut(s) 190
BsrDI GCAATG 2 cut(s) 1256, 1373
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 6 cut(s) 689, 789, 1578, 1634, 1766, 2066
BssECI CCNNGG 5 cut(s) 279, 506, 858, 1179, 1847
BssMI GATC 3 cut(s) 1007, 1021, 1741
BssNI GRCGYC 2 cut(s) 294, 303
BssT1I CCWWGG 3 cut(s) 506, 858, 1179
Bst2UI CCWGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
Bst4CI ACNGT 3 cut(s) 1004, 1423, 2169
Bst6I CTCTTC 1 cut(s) 1097
BstACI GRCGYC 2 cut(s) 294, 303
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 9 cut(s) 456, 649, 656, 718, 773, 988, 1174, 1411, 1550
BstDEI CTNAG 6 cut(s) 251, 1389, 1622, 1904, 1999, 2249
BstENI CCTNNNNNAGG 1 cut(s) 857
BstF5I GGATG 8 cut(s) 208, 255, 363, 471, 1063, 1110, 1824, 1973
BstFNI CGCG 3 cut(s) 700, 720, 930
BstHHI GCGC 4 cut(s) 702, 839, 1528, 1854
BstKTI GATC 3 cut(s) 1010, 1024, 1744
BstMAI GTCTC 2 cut(s) 190, 521
BstMBI GATC 3 cut(s) 1007, 1021, 1741
BstMWI GCNNNNNNNGC 9 cut(s) 455, 699, 1051, 1205, 1460, 1469, 1472, 1558, 1592
BstNI CCWGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
BstPAI GACNNNNGTC 2 cut(s) 1128, 1509
BstSCI CCNGG 8 cut(s) 174, 180, 532, 1188, 1552, 1696, 1750, 1846
BstSFI CTRYAG 1 cut(s) 1035
BstSLI GKGCMC 1 cut(s) 481
BstUI CGCG 3 cut(s) 700, 720, 930
BstV1I GCAGC 9 cut(s) 100, 638, 755, 787, 821, 1195, 1475, 1484, 1579
BstV2I GAAGAC 3 cut(s) 546, 626, 1765
BstX2I RGATCY 1 cut(s) 1007
BstXI CCANNNNNNTGG 2 cut(s) 791, 1773
BstYI RGATCY 1 cut(s) 1007
BsuRI GGCC 9 cut(s) 233, 783, 873, 1116, 1341, 1548, 1825, 1902, 2010
BtgZI GCGATG 2 cut(s) 924, 945
BtrI CACGTC 2 cut(s) 900, 997
BtsCI GGATG 8 cut(s) 208, 255, 363, 471, 1063, 1110, 1824, 1973
BtsIMutI CAGTG 4 cut(s) 423, 796, 1585, 1773
BtuMI TCGCGA 1 cut(s) 930
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 9 cut(s) 456, 649, 656, 718, 773, 988, 1174, 1411, 1550
CaiI CAGNNNCTG 1 cut(s) 181
CfoI GCGC 4 cut(s) 702, 839, 1528, 1854
Cfr13I GGNCC 9 cut(s) 178, 232, 276, 502, 1160, 1295, 1504, 1546, 1631
CseI GACGC 2 cut(s) 283, 682
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 8 cut(s) 400, 442, 892, 943, 1393, 1777, 1799, 2323
CspCI CAANNNNNGTGG 4 cut(s) 607, 642, 1735, 1770
CviAII CATG 8 cut(s) 887, 991, 1337, 1347, 1529, 1813, 2090, 2255
CviQI GTAC 8 cut(s) 400, 442, 892, 943, 1393, 1777, 1799, 2323
DdeI CTNAG 6 cut(s) 251, 1389, 1622, 1904, 1999, 2249
DpnI GATC 3 cut(s) 1009, 1023, 1743
DpnII GATC 3 cut(s) 1007, 1021, 1741
DraIII CACNNNGTG 1 cut(s) 2116
EaeI YGGCCR 2 cut(s) 1339, 2008
Eam1104I CTCTTC 1 cut(s) 1097
EarI CTCTTC 1 cut(s) 1097
EciI GGCGGA 1 cut(s) 1890
Eco130I CCWWGG 3 cut(s) 506, 858, 1179
Eco147I AGGCCT 2 cut(s) 1116, 1825
Eco24I GRGCYC 1 cut(s) 1081
Eco31I GGTCTC 1 cut(s) 190
Eco32I GATATC 2 cut(s) 1694, 2335
Eco47I GGWCC 7 cut(s) 178, 276, 502, 1160, 1295, 1504, 1631
Eco57I CTGAAG 2 cut(s) 447, 1296
Eco88I CYCGRG 2 cut(s) 259, 1136
EcoNI CCTNNNNNAGG 1 cut(s) 857
EcoO109I RGGNCCY 2 cut(s) 178, 502
EcoRI GAATTC 2 cut(s) 2042, 2222
EcoRII CCWGG 8 cut(s) 174, 180, 532, 1188, 1552, 1696, 1750, 1846
EcoRV GATATC 2 cut(s) 1694, 2335
EcoT14I CCWWGG 3 cut(s) 506, 858, 1179
EcoT38I GRGCYC 1 cut(s) 1081
ErhI CCWWGG 3 cut(s) 506, 858, 1179
FaeI CATG 8 cut(s) 890, 994, 1340, 1350, 1532, 1816, 2093, 2258
FalI AAGNNNNNCTT 2 cut(s) 1946, 1978
FaqI GGGAC 5 cut(s) 97, 515, 1281, 1416, 1968
FatI CATG 8 cut(s) 886, 990, 1336, 1346, 1528, 1812, 2089, 2254
FauI CCCGC 1 cut(s) 197
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 1741
FokI GGATG 8 cut(s) 215, 242, 350, 478, 1070, 1117, 1831, 1960
FriOI GRGCYC 1 cut(s) 1081
FspBI CTAG 4 cut(s) 221, 432, 737, 867
FspI TGCGCA 1 cut(s) 838
GlaI GCGC 4 cut(s) 701, 838, 1527, 1853
GsaI CCCAGC 3 cut(s) 118, 453, 2205
GsuI CTGGAG 1 cut(s) 1632
HaeIII GGCC 9 cut(s) 233, 783, 873, 1116, 1341, 1548, 1825, 1902, 2010
HapII CCGG 3 cut(s) 1707, 2007, 2017
HgaI GACGC 2 cut(s) 283, 682
HhaI GCGC 4 cut(s) 702, 839, 1528, 1854
Hin1I GRCGYC 2 cut(s) 294, 303
Hin1II CATG 8 cut(s) 890, 994, 1340, 1350, 1532, 1816, 2093, 2258
Hin6I GCGC 4 cut(s) 700, 837, 1526, 1852
HinP1I GCGC 4 cut(s) 700, 837, 1526, 1852
HincII GTYRAC 3 cut(s) 84, 226, 1639
HindII GTYRAC 3 cut(s) 84, 226, 1639
HindIII AAGCTT 2 cut(s) 5, 639
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 3 cut(s) 1707, 2007, 2017
HphI GGTGA 7 cut(s) 46, 361, 427, 605, 983, 1712, 1751
Hpy166II GTNNAC 8 cut(s) 84, 226, 728, 1000, 1133, 1395, 1639, 1779
Hpy188I TCNGA 9 cut(s) 240, 280, 904, 1066, 1315, 2002, 2072, 2136, 2184
Hpy188III TCNNGA 8 cut(s) 259, 763, 929, 1163, 1214, 1514, 1985, 2330
Hpy8I GTNNAC 8 cut(s) 84, 226, 728, 1000, 1133, 1395, 1639, 1779
Hpy99I CGWCG 4 cut(s) 299, 305, 308, 904
HpyAV CCTTC 5 cut(s) 391, 599, 1012, 1530, 2282
HpyCH4III ACNGT 3 cut(s) 1004, 1423, 2169
HpyCH4IV ACGT 3 cut(s) 303, 899, 996
HpyCH4V TGCA 9 cut(s) 144, 654, 775, 937, 965, 1037, 1378, 2258, 2305
HpyF10VI GCNNNNNNNGC 9 cut(s) 455, 699, 1051, 1205, 1460, 1469, 1472, 1558, 1592
HpyF3I CTNAG 6 cut(s) 251, 1389, 1622, 1904, 1999, 2249
HpySE526I ACGT 3 cut(s) 303, 899, 996
Hsp92I GRCGYC 2 cut(s) 294, 303
Hsp92II CATG 8 cut(s) 890, 994, 1340, 1350, 1532, 1816, 2093, 2258
HspAI GCGC 4 cut(s) 700, 837, 1526, 1852
KflI GGGWCCC 1 cut(s) 502
KpnI GGTACC 2 cut(s) 403, 1802
Ksp22I TGATCA 1 cut(s) 1741
KspAI GTTAAC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 1007, 1021, 1741
LmnI GCTCC 3 cut(s) 134, 1181, 1183
Lsp1109I GCAGC 9 cut(s) 100, 638, 755, 787, 821, 1195, 1475, 1484, 1579
LweI GCATC 5 cut(s) 264, 703, 901, 952, 1686
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 4 cut(s) 221, 432, 737, 867
MaeII ACGT 3 cut(s) 303, 899, 996
MaeIII GTNAC 5 cut(s) 104, 263, 694, 1415, 1768
MalI GATC 3 cut(s) 1009, 1023, 1743
MboI GATC 3 cut(s) 1007, 1021, 1741
MfeI CAATTG 1 cut(s) 1443
MflI RGATCY 1 cut(s) 1007
MhlI GDGCHC 6 cut(s) 481, 981, 1081, 1178, 1188, 2247
MluCI AATT 6 cut(s) 1330, 1399, 1443, 1668, 2042, 2222
MlyI GAGTC 6 cut(s) 89, 101, 180, 243, 1997, 2003
MmeI TCCRAC 3 cut(s) 882, 1938, 2155
MroXI GAANNNNTTC 1 cut(s) 1320
MseI TTAA 8 cut(s) 225, 489, 1367, 1425, 1964, 2021, 2033, 2195
MslI CAYNNNNRTG 3 cut(s) 995, 1817, 2259
MspI CCGG 3 cut(s) 1707, 2007, 2017
MspR9I CCNGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
MunI CAATTG 1 cut(s) 1443
MvaI CCWGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
MvnI CGCG 3 cut(s) 700, 720, 930
MwoI GCNNNNNNNGC 9 cut(s) 455, 699, 1051, 1205, 1460, 1469, 1472, 1558, 1592
NdeI CATATG 1 cut(s) 97
NdeII GATC 3 cut(s) 1007, 1021, 1741
NlaIII CATG 8 cut(s) 890, 994, 1340, 1350, 1532, 1816, 2093, 2258
NmeAIII GCCGAG 1 cut(s) 209
NmuCI GTSAC 4 cut(s) 104, 694, 1415, 1768
NruI TCGCGA 1 cut(s) 930
NsbI TGCGCA 1 cut(s) 838
PaeR7I CTCGAG 1 cut(s) 1136
PaqCI CACCTGC 1 cut(s) 403
PceI AGGCCT 2 cut(s) 1116, 1825
PcsI WCGNNNNNNNCGW 3 cut(s) 236, 297, 717
PdmI GAANNNNTTC 1 cut(s) 1320
PfeI GAWTC 8 cut(s) 241, 315, 596, 1618, 1713, 1863, 1970, 2140
PflFI GACNNNGTC 1 cut(s) 182
PflMI CCANNNNNTGG 1 cut(s) 181
PfoI TCCNGGA 1 cut(s) 174
PleI GAGTC 6 cut(s) 88, 101, 179, 243, 1997, 2002
PpsI GAGTC 6 cut(s) 88, 101, 179, 243, 1997, 2002
PpuMI RGGWCCY 2 cut(s) 178, 502
PshAI GACNNNNGTC 2 cut(s) 1128, 1509
PsiI TTATAA 1 cut(s) 810
Psp5II RGGWCCY 2 cut(s) 178, 502
Psp6I CCWGG 8 cut(s) 174, 180, 532, 1188, 1552, 1696, 1750, 1846
PspFI CCCAGC 3 cut(s) 114, 449, 2201
PspGI CCWGG 8 cut(s) 174, 180, 532, 1188, 1552, 1696, 1750, 1846
PspPI GGNCC 9 cut(s) 178, 232, 276, 502, 1160, 1295, 1504, 1546, 1631
PspPPI RGGWCCY 2 cut(s) 178, 502
PspXI VCTCGAGB 1 cut(s) 1136
PstI CTGCAG 1 cut(s) 1039
PstNI CAGNNNCTG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 1007
PsyI GACNNNGTC 1 cut(s) 182
RruI TCGCGA 1 cut(s) 930
RsaI GTAC 8 cut(s) 401, 443, 893, 944, 1394, 1778, 1800, 2324
RsaNI GTAC 8 cut(s) 400, 442, 892, 943, 1393, 1777, 1799, 2323
RseI CAYNNNNRTG 3 cut(s) 995, 1817, 2259
SaqAI TTAA 8 cut(s) 225, 489, 1367, 1425, 1964, 2021, 2033, 2195
Sau3AI GATC 3 cut(s) 1007, 1021, 1741
Sau96I GGNCC 9 cut(s) 178, 232, 276, 502, 1160, 1295, 1504, 1546, 1631
SchI GAGTC 6 cut(s) 89, 101, 180, 243, 1997, 2003
ScrFI CCNGG 8 cut(s) 176, 182, 534, 1190, 1554, 1698, 1752, 1848
SduI GDGCHC 6 cut(s) 481, 981, 1081, 1178, 1188, 2247
SexAI ACCWGGT 1 cut(s) 180
SfaNI GCATC 5 cut(s) 264, 703, 901, 952, 1686
SfcI CTRYAG 1 cut(s) 1035
Sfr274I CTCGAG 1 cut(s) 1136
SinI GGWCC 7 cut(s) 178, 276, 502, 1160, 1295, 1504, 1631
SlaI CTCGAG 1 cut(s) 1136
SmiMI CAYNNNNRTG 3 cut(s) 995, 1817, 2259
SmlI CTYRAG 4 cut(s) 357, 617, 1136, 1212
SmoI CTYRAG 4 cut(s) 357, 617, 1136, 1212
SpeI ACTAGT 1 cut(s) 220
Sse9I AATT 6 cut(s) 1330, 1399, 1443, 1668, 2042, 2222
SseBI AGGCCT 2 cut(s) 1116, 1825
SsiI CCGC 8 cut(s) 190, 372, 375, 403, 914, 1875, 1938, 2078
SspMI CTAG 4 cut(s) 221, 432, 737, 867
StuI AGGCCT 2 cut(s) 1116, 1825
StyD4I CCNGG 8 cut(s) 174, 180, 532, 1188, 1552, 1696, 1750, 1846
StyI CCWWGG 3 cut(s) 506, 858, 1179
TaaI ACNGT 3 cut(s) 1004, 1423, 2169
TaiI ACGT 3 cut(s) 306, 902, 999
TaqI TCGA 2 cut(s) 711, 1137
TasI AATT 6 cut(s) 1330, 1399, 1443, 1668, 2042, 2222
TatI WGTACW 2 cut(s) 441, 1392
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 8 cut(s) 241, 315, 596, 1618, 1713, 1863, 1970, 2140
Tru1I TTAA 8 cut(s) 225, 489, 1367, 1425, 1964, 2021, 2033, 2195
Tru9I TTAA 8 cut(s) 225, 489, 1367, 1425, 1964, 2021, 2033, 2195
TscAI CASTG 4 cut(s) 430, 796, 1585, 1773
TseFI GTSAC 4 cut(s) 104, 694, 1415, 1768
TseI GCWGC 9 cut(s) 88, 651, 768, 775, 834, 1208, 1463, 1472, 1592
Tsp45I GTSAC 4 cut(s) 104, 694, 1415, 1768
TspDTI ATGAA 6 cut(s) 17, 875, 1419, 1983, 2177, 2215
TspRI CASTG 4 cut(s) 430, 796, 1585, 1773
Tth111I GACNNNGTC 1 cut(s) 182
Van91I CCANNNNNTGG 1 cut(s) 181
VpaK11BI GGWCC 7 cut(s) 178, 276, 502, 1160, 1295, 1504, 1631
XagI CCTNNNNNAGG 1 cut(s) 857
XapI RAATTY 2 cut(s) 2042, 2222
XhoI CTCGAG 1 cut(s) 1136
XmnI GAANNNNTTC 1 cut(s) 1320
XspI CTAG 4 cut(s) 221, 432, 737, 867
ZraI GACGTC 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.