Rh7AG483700

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014594
Physical Location & Seq
Forward (+)
68248326 .. 68254036
5711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG483700.1

Sequence Viewer

Length: 2346 bp
ATGAAGCTCCGAGCTCTCATTCTCATCCCTCTTATCTTCTTCTCCACCCTCATCTTCCTCATTGATCTCACCGAGTCAACTCAGCCCCCATATTCATGTGACTCGTCCAACCCATCAACCGAGTCCTACCCTTTTTGCAAAACAACCCTCCCAATCCACGAAAGAGTCCATGACCTCGTCTCGCGTCTCACATTGGACGAGAAGATCTCCCAACTCGTCAACTCGGCCCCTCCGATTCCCCGACTCGGTATCCCGAGTTACGAGTGGTGGTCGGAGGCTCTTCACGGCGTCGCCGACGTCGGCAAAGGCGTTCGTCTCTATGGCACAGTCTCCAGCGCCACCAGCTTCCCTCAAGTCATTTTAACCGCTGCTTCTTTCAATGAGCATCTCTGGTATCGAATCGGTCAGGCGATCGGAACCGAAGCTAGGGCGGTGTACAATGCAGGACAAGCGACAGGGATGACATTTTGGGCACCAAACATCAACATTTTCAGGGACCCAAGATGGGGAAGAGGGCAAGAGACTCCCGGGGAAGACCCAATGATGACGGGAAAATACGCCGTGGCGTATGTTCGAGGTGTTCAAGGTGATTCATATGAGGGAGGGAAGCTGGCTGGCCATCTCAAGGCCTCGGCTTGCTGTAAGCATTTCACGGCGTACGATCTGGATAGCTGGAATAATGTGACTCGTTTTGGGTTTAATGCAAAGGTAACTCAGCAAGACCTAGCAGACACGTACCAACCTCCATTCAAGAGTTGCGTCGAGCAAGGCAAAGCCAGTGGCATTATGTGTGCTTATAATCAAGTCAATGGAGTCCCAAGCTGTGCAGATCACAATCTCTTAACCAAGACTGCTCGAGGACAATGGGACTTTCACGGGTATATCCATAGGTACATCACGTCGGACTGCGATGCCGTCGGCATCATCCACGACGTGCAGGGATACGCTAAGCAGCCGGAAGATGCCGTCGTCGATGTACTCAAAGCAGGCATGGATGTGAACTGTGGAACCTACTTGCAGAATTACACGAAAAGTGCAGTGCAGCAGAAAAAGCTGCCCATAACATTCATAGACAAAGCCCTCCACAACCTGTTCTCTATCAGGATGAGGTTAGGTCTGTTTGATGGCAACCCAACCAAGCTACTTTTCGGCAACATTGGTCCAGACCAAGTGTGCTCTAAACAGCACCAAGTTTTAGCCCTAGAAGCTGCTGAAGATGGCATTGTGCTTTTGAAGAACGCAGACAAGCTACTCCCACTACCTAAATCAAAGGGGATTTCTCTTGCTGTAATCGGTCCCAATGCCAATGCTTCCGAAACACTTCTCGGAAACTATCATGGCCCACCATGCAAGGTCATTACGCCATTAGAAGGACTGCTAGGTTATGCTAAGAAAACAGTGTATCACCCTGGATGTGATACAGTTAAATGCCCTAATCCTACGATTGATCAAGCTGTGAAAGTAGCAGAACAGGCAGATTATGTGGTTTTGATTGTGGGATTGGACCAAAGTGAAGAGAGAGAGGCGCACGATAGAGACCACTTATACTTGCCTGGTAAGCAACAGCAACTCATTAGCAGTGTGGCTAAAGCTGCCAAGAAACCAGTGATTCTTGTGATTCTCTCTGGGGGTCCAGTTGACATATCTGCAGCCAAGGATAATCCAAAAATCGGAAGCATCTTGTGGGCTGGATACCCAGGTGAAGCTGGTGGAATTGCCCTGGCAGAAATCATCTTTGGTGATCACAACCCAGGTGGAAGACTGCCAGTAACATGGTATACACAAGATTACATCAAAACACCAATGACTGACATGAGGATGAGGCATGACAAAAAATCAGGCTATCCTGGGCGCACTTACAGATTCTACACAGGCGAAAGAGTGTTTGATTTTGGTTATGGCCTCAGCTACTCCAACTACCCTTATAACTTTGTATCTTCATTGACCCAAAAGAAGCTCTACCTAAACGAGTCATCGGTTGGTCTACCAGCTAAGAATTCGGACTCCAGACGCTACCGGTTAGTTTCAGATCTAGGTGAAGAATTCTGTCAGAAGAATTTGTTCAAGGTCACTGTTAGGGCTAAGAATGATGGAGAGATGGCTGGTAAGCATCCGGTGTTGCTATTTGTAAGTCAGAAAAATCCGTCAAATGGAAGTCCAATAAAACAGTTGGTTGGATTCAAAAGCGTGATACTAAGCGCCGGGGAAAAAGCTGAATTTGAATTCATGCTGAACCCTTGTGAGCATTTCAGCCATGCAAATGAGGATGGATCGATGGTGGTAGAAGAAGGTTCTCGTTTCTTGGTTGTGGGTGATGTAGAGCACCCTATTCATGTCATAGTTTGA

Protein Analysis

781

Amino Acids

85.94

Weight (kDa)

7.08

Isoelectric Point (pI)

27.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 109 - 363 1.5e-29 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 408 - 637 4.4e-54 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 706 - 772 5e-12 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014594)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78060
fragaria_vesca FvH4_5g37840
malus_domestica MD08G1179900.v1.1 MD15G1365200.v1.1
prunus_persica Prupe.1G513200_v2.0.a1
pyrus_communis pycom08g15400 pycom15g32750
rosa_chinensis RchiOBHm_Chr7g0239821
rosa_laevigata RLG00000000728
rosa_multiflora Rmu_sc0007193.1_g000008
rosa_roxburghii Rroxscaffold_3G00220790
rosa_rugosa Rorug07G0329500
rosa_samantha Rh7AG483700 Rh7BG456700 Rh7CG501200 Rh7DG469400
rosa_wichuraiana Rw7G040570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 798, 1926
AatII GACGTC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 472
AccI GTMKAC 2 cut(s) 1778, 1984
AccII CGCG 1 cut(s) 184
AciI CCGC 2 cut(s) 366, 431
AclWI GGATC 1 cut(s) 2278
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 5 cut(s) 1996, 2042, 2056, 2216, 2222
AcuI CTGAAG 1 cut(s) 1233
AcyI GRCGYC 2 cut(s) 288, 297
AdeI CACNNNGTG 1 cut(s) 934
AfaI GTAC 5 cut(s) 437, 659, 737, 893, 978
AfiI CCNNNNNNNGG 8 cut(s) 245, 426, 505, 506, 625, 1370, 1670, 2150
AflIII ACRYGT 1 cut(s) 732
AgeI ACCGGT 1 cut(s) 2016
AgsI TTSAA 7 cut(s) 379, 584, 751, 1234, 2065, 2182, 2222
AjiI CACGTC 2 cut(s) 900, 934
AjnI CCWGG 6 cut(s) 1408, 1552, 1696, 1719, 1750, 1846
AjuI GAANNNNNNNTTGG 4 cut(s) 1130, 1162, 1719, 1751
Alw21I GWGCWC 3 cut(s) 16, 1178, 2325
Alw26I GTCTC 6 cut(s) 184, 191, 320, 334, 515, 1530
AlwI GGATC 1 cut(s) 2278
Ama87I CYCGRG 3 cut(s) 253, 527, 855
AoxI GGCC 5 cut(s) 225, 616, 627, 1339, 1900
ApeKI GCWGC 7 cut(s) 368, 952, 1042, 1054, 1208, 1592, 1649
ApoI RAATTY 5 cut(s) 1996, 2042, 2056, 2216, 2222
AsiGI ACCGGT 1 cut(s) 2016
Asp700I GAANNNNTTC 2 cut(s) 1320, 2060
AspLEI GCGC 4 cut(s) 338, 1528, 1854, 2201
AspS9I GGNCC 7 cut(s) 226, 496, 1160, 1295, 1340, 1504, 1631
AsuC2I CCSGG 3 cut(s) 528, 529, 2203
AsuHPI GGTGA 7 cut(s) 61, 599, 1397, 1712, 1751, 2048, 2324
AvaI CYCGRG 3 cut(s) 253, 527, 855
AvaII GGWCC 5 cut(s) 496, 1160, 1295, 1504, 1631
BaeGI GKGCMC 1 cut(s) 475
BaeI ACNNNNGTAYC 2 cut(s) 1684, 1717
BalI TGGCCA 1 cut(s) 618
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 2 cut(s) 540, 1765
Bbv12I GWGCWC 3 cut(s) 16, 1178, 2325
BbvCI CCTCAGC 1 cut(s) 1904
BbvI GCAGC 7 cut(s) 355, 964, 1041, 1054, 1195, 1579, 1661
BccI CCATC 9 cut(s) 121, 498, 627, 1118, 1211, 2084, 2092, 2261, 2269
BceAI ACGGC 5 cut(s) 301, 545, 669, 899, 950
BciT130I CCWGG 6 cut(s) 1410, 1554, 1698, 1721, 1752, 1848
BciVI GTATCC 3 cut(s) 260, 935, 1685
BclI TGATCA 2 cut(s) 1447, 1741
BcnI CCSGG 3 cut(s) 528, 529, 2203
BcoDI GTCTC 6 cut(s) 184, 191, 320, 334, 515, 1530
BfaI CTAG 5 cut(s) 426, 725, 1202, 1379, 2033
BfmI CTRYAG 1 cut(s) 1647
BfoI RGCGCY 2 cut(s) 339, 2202
BfuI GTATCC 3 cut(s) 260, 935, 1685
BglII AGATCT 2 cut(s) 204, 2029
BisI GCNGC 7 cut(s) 369, 953, 1043, 1055, 1209, 1593, 1650
BlpI GCTNAGC 1 cut(s) 948
BlsI GCNGC 7 cut(s) 370, 954, 1044, 1056, 1210, 1594, 1651
Bme1390I CCNGG 9 cut(s) 528, 529, 1410, 1554, 1698, 1721, 1752, 1848, 2203
Bme18I GGWCC 5 cut(s) 496, 1160, 1295, 1504, 1631
BmeT110I CYCGRG 3 cut(s) 253, 527, 855
BmgBI CACGTC 2 cut(s) 900, 934
BmgT120I GGNCC 7 cut(s) 226, 496, 1160, 1295, 1340, 1504, 1631
BmiI GGNNCC 8 cut(s) 228, 418, 474, 497, 498, 1009, 1297, 1632
BmrFI CCNGG 9 cut(s) 528, 529, 1410, 1554, 1698, 1721, 1752, 1848, 2203
BmsI GCATC 6 cut(s) 394, 901, 930, 952, 1686, 2119
BpiI GAAGAC 2 cut(s) 540, 1765
BplI GAGNNNNNCTC 2 cut(s) 191, 223
BpmI CTGGAG 2 cut(s) 316, 1990
Bpu10I CCTNAGC 1 cut(s) 1904
Bpu1102I GCTNAGC 1 cut(s) 948
BpuEI CTTGAG 2 cut(s) 336, 608
BpuMI CCSGG 3 cut(s) 528, 529, 2203
Bsa29I ATCGAT 1 cut(s) 2273
BsaAI YACGTR 1 cut(s) 735
BsaBI GATNNNNATC 1 cut(s) 834
BsaHI GRCGYC 2 cut(s) 288, 297
BsaI GGTCTC 1 cut(s) 1530
BsaWI WCCGGW 2 cut(s) 2016, 2113
Bsc4I CCNNNNNNNGG 8 cut(s) 245, 426, 505, 506, 625, 1370, 1670, 2150
Bse118I RCCGGY 1 cut(s) 2016
Bse1I ACTGG 4 cut(s) 777, 1604, 1634, 1766
Bse8I GATNNNNATC 1 cut(s) 834
BseBI CCWGG 6 cut(s) 1410, 1554, 1698, 1721, 1752, 1848
BseCI ATCGAT 1 cut(s) 2273
BseGI GGATG 9 cut(s) 24, 465, 924, 1000, 1110, 1418, 1824, 2110, 2272
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 8 cut(s) 245, 426, 505, 506, 625, 1370, 1670, 2150
BseMII CTCAG 3 cut(s) 95, 728, 1918
BseNI ACTGG 4 cut(s) 777, 1604, 1634, 1766
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 7 cut(s) 355, 964, 1041, 1054, 1195, 1579, 1661
BsgI GTGCAG 4 cut(s) 846, 956, 1056, 1061
Bsh1236I CGCG 1 cut(s) 184
Bsh1285I CGRYCG 1 cut(s) 414
BshFI GGCC 5 cut(s) 227, 618, 629, 1341, 1902
BshNI GGYRCC 1 cut(s) 472
BshTI ACCGGT 1 cut(s) 2016
BshVI ATCGAT 1 cut(s) 2273
BsiEI CGRYCG 1 cut(s) 414
BsiHKAI GWGCWC 3 cut(s) 16, 1178, 2325
BsiHKCI CYCGRG 3 cut(s) 253, 527, 855
BsiSI CCGG 5 cut(s) 528, 956, 2017, 2114, 2202
BsiWI CGTACG 1 cut(s) 657
BslFI GGGAC 4 cut(s) 509, 800, 881, 1281
BslI CCNNNNNNNGG 8 cut(s) 245, 426, 505, 506, 625, 1370, 1670, 2150
BsmAI GTCTC 6 cut(s) 184, 191, 320, 334, 515, 1530
BsmBI CGTCTC 3 cut(s) 184, 191, 320
BsmFI GGGAC 4 cut(s) 509, 800, 881, 1281
BsnI GGCC 5 cut(s) 227, 618, 629, 1341, 1902
Bso31I GGTCTC 1 cut(s) 1530
BsoBI CYCGRG 3 cut(s) 253, 527, 855
Bsp1286I GDGCHC 4 cut(s) 16, 475, 1178, 2325
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 9 cut(s) 64, 204, 411, 661, 829, 1447, 1741, 2029, 2270
Bsp1720I GCTNAGC 1 cut(s) 948
BspACI CCGC 2 cut(s) 366, 431
BspANI GGCC 5 cut(s) 227, 618, 629, 1341, 1902
BspCNI CTCAG 3 cut(s) 94, 727, 1917
BspDI ATCGAT 1 cut(s) 2273
BspFNI CGCG 1 cut(s) 184
BspLI GGNNCC 8 cut(s) 228, 418, 474, 497, 498, 1009, 1297, 1632
BspMAI CTGCAG 1 cut(s) 1651
BspPI GGATC 1 cut(s) 2278
BspQI GCTCTTC 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 472
BspTNI GGTCTC 1 cut(s) 1530
BsrFI RCCGGY 1 cut(s) 2016
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 4 cut(s) 777, 1604, 1634, 1766
BssAI RCCGGY 1 cut(s) 2016
BssMI GATC 9 cut(s) 64, 204, 411, 661, 829, 1447, 1741, 2029, 2270
BssNAI GTATAC 1 cut(s) 1779
BssNI GRCGYC 2 cut(s) 288, 297
BssT1I CCWWGG 1 cut(s) 1653
Bst1107I GTATAC 1 cut(s) 1779
Bst2UI CCWGG 6 cut(s) 1410, 1554, 1698, 1721, 1752, 1848
Bst4CI ACNGT 6 cut(s) 328, 1004, 1399, 1423, 2074, 2169
Bst6I CTCTTC 3 cut(s) 285, 505, 1509
BstACI GRCGYC 2 cut(s) 288, 297
BstAUI TGTACA 1 cut(s) 435
BstBAI YACGTR 1 cut(s) 735
BstC8I GCNNGC 4 cut(s) 612, 616, 637, 988
BstDEI CTNAG 8 cut(s) 81, 714, 948, 1389, 1904, 1992, 2082, 2195
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 9 cut(s) 24, 465, 924, 1000, 1110, 1418, 1824, 2110, 2272
BstFNI CGCG 1 cut(s) 184
BstH2I RGCGCY 2 cut(s) 339, 2202
BstHHI GCGC 4 cut(s) 338, 1528, 1854, 2201
BstKTI GATC 9 cut(s) 67, 207, 414, 664, 832, 1450, 1744, 2032, 2273
BstMAI GTCTC 6 cut(s) 184, 191, 320, 334, 515, 1530
BstMBI GATC 9 cut(s) 64, 204, 411, 661, 829, 1447, 1741, 2029, 2270
BstMCI CGRYCG 1 cut(s) 414
BstMWI GCNNNNNNNGC 8 cut(s) 342, 449, 1051, 1205, 1347, 1472, 1558, 1592
BstNI CCWGG 6 cut(s) 1410, 1554, 1698, 1721, 1752, 1848
BstSCI CCNGG 9 cut(s) 526, 527, 1408, 1552, 1696, 1719, 1750, 1846, 2201
BstSFI CTRYAG 1 cut(s) 1647
BstSLI GKGCMC 1 cut(s) 475
BstUI CGCG 1 cut(s) 184
BstV1I GCAGC 7 cut(s) 355, 964, 1041, 1054, 1195, 1579, 1661
BstV2I GAAGAC 2 cut(s) 540, 1765
BstX2I RGATCY 2 cut(s) 204, 2029
BstXI CCANNNNNNTGG 1 cut(s) 1773
BstYI RGATCY 2 cut(s) 204, 2029
BstZ17I GTATAC 1 cut(s) 1779
Bsu15I ATCGAT 1 cut(s) 2273
BsuI GTATCC 3 cut(s) 260, 935, 1685
BsuRI GGCC 5 cut(s) 227, 618, 629, 1341, 1902
BsuTUI ATCGAT 1 cut(s) 2273
BtgI CCRYGG 1 cut(s) 561
BtgZI GCGATG 1 cut(s) 924
BtrI CACGTC 2 cut(s) 900, 934
BtsCI GGATG 9 cut(s) 24, 465, 924, 1000, 1110, 1418, 1824, 2110, 2272
BtsI GCAGTG 2 cut(s) 1044, 1585
BtsIMutI CAGTG 6 cut(s) 784, 1044, 1404, 1585, 1611, 2070
Cac8I GCNNGC 4 cut(s) 612, 616, 637, 988
CfoI GCGC 4 cut(s) 338, 1528, 1854, 2201
Cfr10I RCCGGY 1 cut(s) 2016
Cfr13I GGNCC 7 cut(s) 226, 496, 1160, 1295, 1340, 1504, 1631
Cfr9I CCCGGG 1 cut(s) 527
ClaI ATCGAT 1 cut(s) 2273
CseI GACGC 4 cut(s) 173, 277, 748, 2019
Csp6I GTAC 5 cut(s) 436, 658, 736, 892, 977
CspAI ACCGGT 1 cut(s) 2016
CspCI CAANNNNNGTGG 4 cut(s) 760, 795, 1735, 1770
CviQI GTAC 5 cut(s) 436, 658, 736, 892, 977
DdeI CTNAG 8 cut(s) 81, 714, 948, 1389, 1904, 1992, 2082, 2195
DpnI GATC 9 cut(s) 66, 206, 413, 663, 831, 1449, 1743, 2031, 2272
DpnII GATC 9 cut(s) 64, 204, 411, 661, 829, 1447, 1741, 2029, 2270
DraIII CACNNNGTG 1 cut(s) 934
EaeI YGGCCR 1 cut(s) 616
Eam1104I CTCTTC 3 cut(s) 285, 505, 1509
EarI CTCTTC 3 cut(s) 285, 505, 1509
Ecl136II GAGCTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 1653
Eco147I AGGCCT 1 cut(s) 629
Eco24I GRGCYC 1 cut(s) 16
Eco31I GGTCTC 1 cut(s) 1530
Eco47I GGWCC 5 cut(s) 496, 1160, 1295, 1504, 1631
Eco53kI GAGCTC 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 1233
Eco88I CYCGRG 3 cut(s) 253, 527, 855
EcoICRI GAGCTC 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 496
EcoRI GAATTC 3 cut(s) 1996, 2042, 2222
EcoRII CCWGG 6 cut(s) 1408, 1552, 1696, 1719, 1750, 1846
EcoT14I CCWWGG 1 cut(s) 1653
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 1653
Esp3I CGTCTC 3 cut(s) 184, 191, 320
FaqI GGGAC 4 cut(s) 509, 800, 881, 1281
FauNDI CATATG 1 cut(s) 595
FbaI TGATCA 2 cut(s) 1447, 1741
FblI GTMKAC 2 cut(s) 1778, 1984
Fnu4HI GCNGC 7 cut(s) 369, 953, 1043, 1055, 1209, 1593, 1650
FokI GGATG 9 cut(s) 11, 472, 911, 1007, 1117, 1425, 1831, 2097, 2279
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 7 cut(s) 369, 953, 1043, 1055, 1209, 1593, 1650
FspBI CTAG 5 cut(s) 426, 725, 1202, 1379, 2033
GlaI GCGC 4 cut(s) 337, 1527, 1853, 2200
GluI GCNGC 7 cut(s) 369, 953, 1043, 1055, 1209, 1593, 1650
GsuI CTGGAG 2 cut(s) 316, 1990
HaeII RGCGCY 2 cut(s) 339, 2202
HaeIII GGCC 5 cut(s) 227, 618, 629, 1341, 1902
HapII CCGG 5 cut(s) 528, 956, 2017, 2114, 2202
HgaI GACGC 4 cut(s) 173, 277, 748, 2019
HhaI GCGC 4 cut(s) 338, 1528, 1854, 2201
Hin1I GRCGYC 2 cut(s) 288, 297
Hin6I GCGC 4 cut(s) 336, 1526, 1852, 2199
HinP1I GCGC 4 cut(s) 336, 1526, 1852, 2199
HincII GTYRAC 3 cut(s) 78, 220, 1639
HindII GTYRAC 3 cut(s) 78, 220, 1639
HpaII CCGG 5 cut(s) 528, 956, 2017, 2114, 2202
HphI GGTGA 7 cut(s) 61, 599, 1397, 1712, 1751, 2048, 2324
Hpy166II GTNNAC 7 cut(s) 78, 220, 436, 1000, 1639, 1779, 1985
Hpy188III TCNNGA 6 cut(s) 253, 665, 751, 1102, 1163, 2007
Hpy8I GTNNAC 7 cut(s) 78, 220, 436, 1000, 1639, 1779, 1985
Hpy99I CGWCG 9 cut(s) 293, 299, 302, 764, 904, 920, 935, 971, 974
HpyAV CCTTC 2 cut(s) 1364, 2282
HpyCH4III ACNGT 6 cut(s) 328, 1004, 1399, 1423, 2074, 2169
HpyCH4IV ACGT 4 cut(s) 297, 734, 899, 933
HpyF10VI GCNNNNNNNGC 8 cut(s) 342, 449, 1051, 1205, 1347, 1472, 1558, 1592
HpyF3I CTNAG 8 cut(s) 81, 714, 948, 1389, 1904, 1992, 2082, 2195
HpySE526I ACGT 4 cut(s) 297, 734, 899, 933
Hsp92I GRCGYC 2 cut(s) 288, 297
HspAI GCGC 4 cut(s) 336, 1526, 1852, 2199
KflI GGGWCCC 1 cut(s) 496
Ksp22I TGATCA 2 cut(s) 1447, 1741
Kzo9I GATC 9 cut(s) 64, 204, 411, 661, 829, 1447, 1741, 2029, 2270
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 12
Lsp1109I GCAGC 7 cut(s) 355, 964, 1041, 1054, 1195, 1579, 1661
LweI GCATC 6 cut(s) 394, 901, 930, 952, 1686, 2119
MaeI CTAG 5 cut(s) 426, 725, 1202, 1379, 2033
MaeII ACGT 4 cut(s) 297, 734, 899, 933
MaeIII GTNAC 6 cut(s) 98, 257, 682, 709, 1768, 2068
MalI GATC 9 cut(s) 66, 206, 413, 663, 831, 1449, 1743, 2031, 2272
MboI GATC 9 cut(s) 64, 204, 411, 661, 829, 1447, 1741, 2029, 2270
MflI RGATCY 2 cut(s) 204, 2029
MhlI GDGCHC 4 cut(s) 16, 475, 1178, 2325
MlsI TGGCCA 1 cut(s) 618
MluCI AATT 7 cut(s) 1021, 1713, 1996, 2042, 2056, 2216, 2222
MluNI TGGCCA 1 cut(s) 618
MmeI TCCRAC 5 cut(s) 132, 252, 882, 1938, 2155
Mox20I TGGCCA 1 cut(s) 618
MroXI GAANNNNTTC 2 cut(s) 1320, 2060
MscI TGGCCA 1 cut(s) 618
MseI TTAA 4 cut(s) 362, 699, 842, 1425
MslI CAYNNNNRTG 4 cut(s) 94, 995, 1817, 2259
Msp20I TGGCCA 1 cut(s) 618
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 5 cut(s) 528, 956, 2017, 2114, 2202
MspR9I CCNGG 9 cut(s) 528, 529, 1410, 1554, 1698, 1721, 1752, 1848, 2203
MvaI CCWGG 6 cut(s) 1410, 1554, 1698, 1721, 1752, 1848
MvnI CGCG 1 cut(s) 184
MwoI GCNNNNNNNGC 8 cut(s) 342, 449, 1051, 1205, 1347, 1472, 1558, 1592
NciI CCSGG 3 cut(s) 528, 529, 2203
NdeI CATATG 1 cut(s) 595
NdeII GATC 9 cut(s) 64, 204, 411, 661, 829, 1447, 1741, 2029, 2270
NlaIV GGNNCC 8 cut(s) 228, 418, 474, 497, 498, 1009, 1297, 1632
NmeAIII GCCGAG 2 cut(s) 203, 611
NmuCI GTSAC 3 cut(s) 98, 682, 2068
PaeR7I CTCGAG 1 cut(s) 855
PceI AGGCCT 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 285
PcsI WCGNNNNNNNCGW 3 cut(s) 230, 291, 306
PdmI GAANNNNTTC 2 cut(s) 1320, 2060
PfeI GAWTC 7 cut(s) 235, 399, 590, 1609, 1618, 1863, 2178
Pfl23II CGTACG 1 cut(s) 657
PflFI GACNNNGTC 1 cut(s) 176
PinAI ACCGGT 1 cut(s) 2016
PkrI GCNGC 7 cut(s) 370, 954, 1044, 1056, 1210, 1594, 1651
Ple19I CGATCG 1 cut(s) 414
Ppu21I YACGTR 1 cut(s) 735
PpuMI RGGWCCY 1 cut(s) 496
PsiI TTATAA 2 cut(s) 798, 1926
Psp124BI GAGCTC 1 cut(s) 16
Psp5II RGGWCCY 1 cut(s) 496
Psp6I CCWGG 6 cut(s) 1408, 1552, 1696, 1719, 1750, 1846
PspGI CCWGG 6 cut(s) 1408, 1552, 1696, 1719, 1750, 1846
PspLI CGTACG 1 cut(s) 657
PspN4I GGNNCC 8 cut(s) 228, 418, 474, 497, 498, 1009, 1297, 1632
PspPI GGNCC 7 cut(s) 226, 496, 1160, 1295, 1340, 1504, 1631
PspPPI RGGWCCY 1 cut(s) 496
PspXI VCTCGAGB 1 cut(s) 855
PstI CTGCAG 1 cut(s) 1651
PsuI RGATCY 2 cut(s) 204, 2029
PsyI GACNNNGTC 1 cut(s) 176
PvuI CGATCG 1 cut(s) 414
RsaI GTAC 5 cut(s) 437, 659, 737, 893, 978
RsaNI GTAC 5 cut(s) 436, 658, 736, 892, 977
RseI CAYNNNNRTG 4 cut(s) 94, 995, 1817, 2259
SacI GAGCTC 1 cut(s) 16
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 4 cut(s) 362, 699, 842, 1425
SatI GCNGC 7 cut(s) 369, 953, 1043, 1055, 1209, 1593, 1650
Sau3AI GATC 9 cut(s) 64, 204, 411, 661, 829, 1447, 1741, 2029, 2270
Sau96I GGNCC 7 cut(s) 226, 496, 1160, 1295, 1340, 1504, 1631
ScrFI CCNGG 9 cut(s) 528, 529, 1410, 1554, 1698, 1721, 1752, 1848, 2203
SduI GDGCHC 4 cut(s) 16, 475, 1178, 2325
SfaNI GCATC 6 cut(s) 394, 901, 930, 952, 1686, 2119
SfcI CTRYAG 1 cut(s) 1647
Sfr274I CTCGAG 1 cut(s) 855
SinI GGWCC 5 cut(s) 496, 1160, 1295, 1504, 1631
SlaI CTCGAG 1 cut(s) 855
SmaI CCCGGG 1 cut(s) 529
SmiMI CAYNNNNRTG 4 cut(s) 94, 995, 1817, 2259
SmlI CTYRAG 3 cut(s) 351, 623, 855
SmoI CTYRAG 3 cut(s) 351, 623, 855
Sse9I AATT 7 cut(s) 1021, 1713, 1996, 2042, 2056, 2216, 2222
SseBI AGGCCT 1 cut(s) 629
SsiI CCGC 2 cut(s) 366, 431
SspMI CTAG 5 cut(s) 426, 725, 1202, 1379, 2033
SstI GAGCTC 1 cut(s) 16
StuI AGGCCT 1 cut(s) 629
StyD4I CCNGG 9 cut(s) 526, 527, 1408, 1552, 1696, 1719, 1750, 1846, 2201
StyI CCWWGG 1 cut(s) 1653
TaaI ACNGT 6 cut(s) 328, 1004, 1399, 1423, 2074, 2169
TaiI ACGT 4 cut(s) 300, 737, 902, 936
TaqI TCGA 6 cut(s) 397, 574, 762, 856, 972, 2273
TaqII GACCGA 2 cut(s) 392, 1283
TasI AATT 7 cut(s) 1021, 1713, 1996, 2042, 2056, 2216, 2222
TatI WGTACW 2 cut(s) 435, 976
TfiI GAWTC 7 cut(s) 235, 399, 590, 1609, 1618, 1863, 2178
Tru1I TTAA 4 cut(s) 362, 699, 842, 1425
Tru9I TTAA 4 cut(s) 362, 699, 842, 1425
TscAI CASTG 6 cut(s) 784, 1044, 1404, 1585, 1611, 2077
TseFI GTSAC 3 cut(s) 98, 682, 2068
TseI GCWGC 7 cut(s) 368, 952, 1042, 1054, 1208, 1592, 1649
Tsp45I GTSAC 3 cut(s) 98, 682, 2068
TspDTI ATGAA 7 cut(s) 17, 84, 582, 1057, 1929, 2215, 2321
TspGWI ACGGA 1 cut(s) 2133
TspMI CCCGGG 1 cut(s) 527
TspRI CASTG 6 cut(s) 784, 1044, 1404, 1585, 1611, 2077
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 5 cut(s) 496, 1160, 1295, 1504, 1631
XapI RAATTY 5 cut(s) 1996, 2042, 2056, 2216, 2222
XhoI CTCGAG 1 cut(s) 855
XmaI CCCGGG 1 cut(s) 527
XmiI GTMKAC 2 cut(s) 1778, 1984
XmnI GAANNNNTTC 2 cut(s) 1320, 2060
XspI CTAG 5 cut(s) 426, 725, 1202, 1379, 2033
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.