Rw7G040570

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014594
Physical Location & Seq
Forward (+)
63217537 .. 63223659
6123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040570.1

Sequence Viewer

Length: 2469 bp
ATGAGGAAAGGTGCCCTGAAAATTAACAGTCGATTTAGTGGGAGACCTAGCTTGGTCCATCCCCAGCTGCTATATTACTCTCACTCTCTTTCTGCTTTAATTTCTGCATCTCAAACAAAAACCCCACACTCGAAAATGAAGCTCCGAGCTCTCATTCTCATCCCTGTTATCTTCTTCTCCACCCTCATCTTCCTCATTGATCTCACCGAGTCAACTCAGCCCCCATATTCATGTGACTCGTCCAACCCATCAACCGAGTCCTACCCTTTTTGCAAAACAACCCTCCCAATCCACGAAAGAGTCCATGACCTCGTCTCGCGTCTCACATTGGACGAGAAGATCTCCCAACTCGTCAACTCGGCCCCTCCGATTCCCCGACTCGGTATCCCGAGTTACGAGTGGTGGTCGGAGGCTCTTCACGGCGTCGCCGACGTCGGCAAAGGCGTTCGTCTCTATGGCACCGTCTCCAGCGCCACCAGCTTCCCTCAAGTCATTTTAACCGCTGCTTCATTCAATGAGCATCTCTGGTACAGAATCGGTCAGGCGATCGGAACCGAAGCTAGGGCGGTGTACAATGCAGGACAAGCGACAGGGATGACATTTTGGGCACCAAACATCAACATTTTCAGGGACCCAAGATGGGGAAGAGGGCAAGAGACTCCCGGGGAAGACCCAATGATGACGGGAAAATACGCCGTGGCGTATGTTCGAGGTGTTCAAGGTGATTCATATGAGGGAGGGAAGCTGGCTGGCCATCTCAAGGCCTCGGCTTGCTGTAAGCATTTCACGGCGTACGATCTGGATAGCTGGAATAATGTGACTCGTTTTGGGTTTAATGCAAAGGTGACTCAGCAAGACCTAGCAGACACGTACCAACCTCCATTCAAGAGTTGCGTCGAGCAGGGCAAAGCCAGTGGCATTATGTGTGCTTATAATCAAGTCAATGGAGTCCCAAGCTGTGCAGATCGCAATCTCTTAACCAAGACTGCTCGAGGACAATGGGACTTTCACGGGTACATCACGTCGGACTGCGATGCCGTCGGCATCATCCACGACGTGCAGGGATACGCTAAGCAGCCGGAAGATGCCGTCGTCGATGTACTCAAAGCAGGCATGGATGTGAACTGTGGAACCTACTTGCAGAATTACACGAAAAGTGCAGTGCAGCAGAAAAAGCTGCCCATAACATACATAGACAAAGCCCTCCACAACCTGTTCTCTATAAGGATGAGGTTAGGTCTGTTTGATGGCAACCCAACCAAGCTACTTTTCGGCAACATTGGTCCAGACCAAGTGTGCTCTAAACAGCACCAAGTTTTAGCCCTAGAAGCTGCAGAAGATGGCATTGTGCTTTTGAAGAACGCAGACAAGCTACTCCCACTACCTAAATCAAAGGGGATTTCTCTTGCTGTAATCGGTCCCAATGCCAATGCTTCCGAAACACTTCTCGGAAACTATCATGGCCCACCATGCAAGGTCATTACGCCATTAGAAGGACTGCTAGGTTATGCTAAGAAAACAGTGTATCACCCTGGATGTGATACAGTTAAATGCCCTAATCCTACGATTGATCAAGCTGTGAAAGTGGCAGAACAGGCAGATTATGTGGTTTTGATTGTGGGATTGGACCAAAGTGAAGAGAGAGAGGCGCACGATAGAGACCACTTATACTTGCCTGGTAAGCAACAGCAACTCATTAGCAGTGTGGCTAAAGCTGCCAAGAAACCAGTGATTCTTGTGATTCTCTCTGGGGGTCCAGTTGACATATCTGCAGCCAAGGATAATCCAAAAATAGGAAGCATCTTGTGGGCTGGATACCCAGGTGAAGCTGGTGGAATTGCCCTGGCAGAAATCATCTTTGGTGATCACAACCCAGGTGGAAGATTGCCAGTAACATGGTATACACAAGATTACATCAAAACACCAATGACTGACATGAGGATGAGGCATGACAAAAAATCAGGCTATCCTGGGCGCACTTACAGATTCTACACAGGCGAAAGAGTGTTTGATTTCGGTTATGGCCTCAGCTACTCCAACTACGCTTATCACTTTGTATCTTCATTAACCCAAAAGAACCTCTACCTAAATGAGTCATCGGTTGGTCTACCAGCTAAGAATTCGGACTCTAGACGCTACCAGTTAGTTTCAGATCTAGGTGAAGAATTCTGTCAGAAGAATTTGTTCAAGGTCACTGTTAGGGCTAAGAATGATGGAGAGATGGCTGGTAAGCATCCAGTGTTGCTGTTTGTAAGTCAGAAAAATCCGTCAAATGGAAGTCCAATAAAACAGTTGGTTGGATTCAAAAGCGTGATACTAAGCGCCGGGGAAAAAGCTGAATTTGAATTCATGCTGAACCCTTGTGAGCATTTCAGCCATGCAAATGAGGATGGATCGATGGTGGTAGAAGAAGGTTCTCGTTTCTTGGTTGTGGGTGATGTAGAGCACCCTATTCATGTCATAGTTTGA

Protein Analysis

822

Amino Acids

90.32

Weight (kDa)

8.17

Isoelectric Point (pI)

28.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 154 - 405 1.4e-32 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 449 - 678 4.8e-54 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 747 - 813 5.3e-12 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014594)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78060
fragaria_vesca FvH4_5g37840
malus_domestica MD08G1179900.v1.1 MD15G1365200.v1.1
prunus_persica Prupe.1G513200_v2.0.a1
pyrus_communis pycom08g15400 pycom15g32750
rosa_chinensis RchiOBHm_Chr7g0239821
rosa_laevigata RLG00000000728
rosa_multiflora Rmu_sc0007193.1_g000008
rosa_roxburghii Rroxscaffold_3G00220790
rosa_rugosa Rorug07G0329500
rosa_samantha Rh7AG483700 Rh7BG456700 Rh7CG501200 Rh7DG469400
rosa_wichuraiana Rw7G040570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
AatII GACGTC 1 cut(s) 435
AccB1I GGYRCC 3 cut(s) 11, 458, 607
AccI GTMKAC 2 cut(s) 1901, 2107
AccII CGCG 1 cut(s) 319
AciI CCGC 2 cut(s) 501, 566
AclWI GGATC 1 cut(s) 2401
AcoI YGGCCR 1 cut(s) 751
AcsI RAATTY 5 cut(s) 2119, 2165, 2179, 2339, 2345
AcyI GRCGYC 2 cut(s) 423, 432
AdeI CACNNNGTG 1 cut(s) 1057
AfaI GTAC 6 cut(s) 530, 572, 794, 872, 1016, 1101
AfiI CCNNNNNNNGG 8 cut(s) 380, 561, 640, 641, 760, 1493, 1793, 2273
AflIII ACRYGT 1 cut(s) 867
AgsI TTSAA 7 cut(s) 514, 719, 886, 1357, 2188, 2305, 2345
AjiI CACGTC 2 cut(s) 1023, 1057
AjnI CCWGG 6 cut(s) 1531, 1675, 1819, 1842, 1873, 1969
AjuI GAANNNNNNNTTGG 4 cut(s) 1253, 1285, 1842, 1874
Alw21I GWGCWC 3 cut(s) 151, 1301, 2448
Alw26I GTCTC 7 cut(s) 37, 319, 326, 455, 469, 650, 1653
AlwI GGATC 1 cut(s) 2401
Ama87I CYCGRG 3 cut(s) 388, 662, 990
AoxI GGCC 5 cut(s) 360, 751, 762, 1462, 2023
ApeKI GCWGC 8 cut(s) 67, 503, 1075, 1165, 1177, 1331, 1715, 1772
ApoI RAATTY 5 cut(s) 2119, 2165, 2179, 2339, 2345
Asp700I GAANNNNTTC 2 cut(s) 1443, 2183
AspLEI GCGC 4 cut(s) 473, 1651, 1977, 2324
AspS9I GGNCC 8 cut(s) 55, 361, 631, 1283, 1418, 1463, 1627, 1754
AsuC2I CCSGG 3 cut(s) 663, 664, 2326
AsuHPI GGTGA 8 cut(s) 196, 734, 856, 1520, 1835, 1874, 2171, 2447
AvaI CYCGRG 3 cut(s) 388, 662, 990
AvaII GGWCC 6 cut(s) 55, 631, 1283, 1418, 1627, 1754
BaeGI GKGCMC 2 cut(s) 16, 610
BaeI ACNNNNGTAYC 2 cut(s) 1807, 1840
BalI TGGCCA 1 cut(s) 753
BanI GGYRCC 3 cut(s) 11, 458, 607
BanII GRGCYC 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 675
Bbv12I GWGCWC 3 cut(s) 151, 1301, 2448
BbvCI CCTCAGC 1 cut(s) 2027
BbvI GCAGC 8 cut(s) 54, 490, 1087, 1164, 1177, 1318, 1702, 1784
BceAI ACGGC 5 cut(s) 436, 680, 804, 1022, 1073
BciT130I CCWGG 6 cut(s) 1533, 1677, 1821, 1844, 1875, 1971
BciVI GTATCC 3 cut(s) 395, 1058, 1808
BclI TGATCA 2 cut(s) 1570, 1864
BcnI CCSGG 3 cut(s) 663, 664, 2326
BcoDI GTCTC 7 cut(s) 37, 319, 326, 455, 469, 650, 1653
BfaI CTAG 7 cut(s) 48, 561, 860, 1325, 1502, 2130, 2156
BfmI CTRYAG 2 cut(s) 1332, 1770
BfoI RGCGCY 2 cut(s) 474, 2325
BfuI GTATCC 3 cut(s) 395, 1058, 1808
BglII AGATCT 2 cut(s) 339, 2152
BisI GCNGC 8 cut(s) 68, 504, 1076, 1166, 1178, 1332, 1716, 1773
BlpI GCTNAGC 1 cut(s) 1071
BlsI GCNGC 8 cut(s) 69, 505, 1077, 1167, 1179, 1333, 1717, 1774
Bme1390I CCNGG 9 cut(s) 663, 664, 1533, 1677, 1821, 1844, 1875, 1971, 2326
Bme18I GGWCC 6 cut(s) 55, 631, 1283, 1418, 1627, 1754
BmeT110I CYCGRG 3 cut(s) 388, 662, 990
BmgBI CACGTC 2 cut(s) 1023, 1057
BmgT120I GGNCC 8 cut(s) 55, 361, 631, 1283, 1418, 1463, 1627, 1754
BmrFI CCNGG 9 cut(s) 663, 664, 1533, 1677, 1821, 1844, 1875, 1971, 2326
BmsI GCATC 7 cut(s) 116, 529, 1024, 1053, 1075, 1809, 2242
BpiI GAAGAC 1 cut(s) 675
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 451
Bpu10I CCTNAGC 1 cut(s) 2027
Bpu1102I GCTNAGC 1 cut(s) 1071
BpuEI CTTGAG 2 cut(s) 471, 743
BpuMI CCSGG 3 cut(s) 663, 664, 2326
Bsa29I ATCGAT 1 cut(s) 2396
BsaAI YACGTR 1 cut(s) 870
BsaBI GATNNNNATC 1 cut(s) 969
BsaHI GRCGYC 2 cut(s) 423, 432
BsaI GGTCTC 2 cut(s) 37, 1653
Bsc4I CCNNNNNNNGG 8 cut(s) 380, 561, 640, 641, 760, 1493, 1793, 2273
Bse1I ACTGG 6 cut(s) 912, 1727, 1757, 1889, 2140, 2237
Bse8I GATNNNNATC 1 cut(s) 969
BseBI CCWGG 6 cut(s) 1533, 1677, 1821, 1844, 1875, 1971
BseCI ATCGAT 1 cut(s) 2396
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 8 cut(s) 380, 561, 640, 641, 760, 1493, 1793, 2273
BseMII CTCAG 3 cut(s) 230, 863, 2041
BseNI ACTGG 6 cut(s) 912, 1727, 1757, 1889, 2140, 2237
BseSI GKGCMC 2 cut(s) 16, 610
BseXI GCAGC 8 cut(s) 54, 490, 1087, 1164, 1177, 1318, 1702, 1784
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 4 cut(s) 981, 1079, 1179, 1184
Bsh1236I CGCG 1 cut(s) 319
Bsh1285I CGRYCG 1 cut(s) 549
BshFI GGCC 5 cut(s) 362, 753, 764, 1464, 2025
BshNI GGYRCC 3 cut(s) 11, 458, 607
BshVI ATCGAT 1 cut(s) 2396
BsiEI CGRYCG 1 cut(s) 549
BsiHKAI GWGCWC 3 cut(s) 151, 1301, 2448
BsiHKCI CYCGRG 3 cut(s) 388, 662, 990
BsiSI CCGG 3 cut(s) 663, 1079, 2325
BsiWI CGTACG 1 cut(s) 792
BslFI GGGAC 4 cut(s) 644, 935, 1016, 1404
BslI CCNNNNNNNGG 8 cut(s) 380, 561, 640, 641, 760, 1493, 1793, 2273
BsmAI GTCTC 7 cut(s) 37, 319, 326, 455, 469, 650, 1653
BsmBI CGTCTC 4 cut(s) 319, 326, 455, 469
BsmFI GGGAC 4 cut(s) 644, 935, 1016, 1404
BsnI GGCC 5 cut(s) 362, 753, 764, 1464, 2025
Bso31I GGTCTC 2 cut(s) 37, 1653
BsoBI CYCGRG 3 cut(s) 388, 662, 990
Bsp1286I GDGCHC 5 cut(s) 16, 151, 610, 1301, 2448
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 9 cut(s) 199, 339, 546, 796, 964, 1570, 1864, 2152, 2393
Bsp1720I GCTNAGC 1 cut(s) 1071
BspACI CCGC 2 cut(s) 501, 566
BspANI GGCC 5 cut(s) 362, 753, 764, 1464, 2025
BspCNI CTCAG 3 cut(s) 229, 862, 2040
BspDI ATCGAT 1 cut(s) 2396
BspFNI CGCG 1 cut(s) 319
BspMAI CTGCAG 2 cut(s) 1336, 1774
BspPI GGATC 1 cut(s) 2401
BspQI GCTCTTC 1 cut(s) 420
BspT107I GGYRCC 3 cut(s) 11, 458, 607
BspTNI GGTCTC 2 cut(s) 37, 1653
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 6 cut(s) 912, 1727, 1757, 1889, 2140, 2237
BssMI GATC 9 cut(s) 199, 339, 546, 796, 964, 1570, 1864, 2152, 2393
BssNAI GTATAC 1 cut(s) 1902
BssNI GRCGYC 2 cut(s) 423, 432
BssT1I CCWWGG 1 cut(s) 1776
Bst1107I GTATAC 1 cut(s) 1902
Bst2UI CCWGG 6 cut(s) 1533, 1677, 1821, 1844, 1875, 1971
Bst4CI ACNGT 7 cut(s) 29, 463, 1127, 1522, 1546, 2197, 2292
Bst6I CTCTTC 3 cut(s) 420, 640, 1632
BstACI GRCGYC 2 cut(s) 423, 432
BstAUI TGTACA 1 cut(s) 570
BstBAI YACGTR 1 cut(s) 870
BstC8I GCNNGC 4 cut(s) 747, 751, 772, 1111
BstDEI CTNAG 8 cut(s) 216, 849, 1071, 1512, 2027, 2115, 2205, 2318
BstDSI CCRYGG 1 cut(s) 696
BstFNI CGCG 1 cut(s) 319
BstH2I RGCGCY 2 cut(s) 474, 2325
BstHHI GCGC 4 cut(s) 473, 1651, 1977, 2324
BstKTI GATC 9 cut(s) 202, 342, 549, 799, 967, 1573, 1867, 2155, 2396
BstMAI GTCTC 7 cut(s) 37, 319, 326, 455, 469, 650, 1653
BstMBI GATC 9 cut(s) 199, 339, 546, 796, 964, 1570, 1864, 2152, 2393
BstMCI CGRYCG 1 cut(s) 549
BstMWI GCNNNNNNNGC 8 cut(s) 477, 584, 1174, 1328, 1470, 1595, 1681, 1715
BstNI CCWGG 6 cut(s) 1533, 1677, 1821, 1844, 1875, 1971
BstSCI CCNGG 9 cut(s) 661, 662, 1531, 1675, 1819, 1842, 1873, 1969, 2324
BstSFI CTRYAG 2 cut(s) 1332, 1770
BstSLI GKGCMC 2 cut(s) 16, 610
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 8 cut(s) 54, 490, 1087, 1164, 1177, 1318, 1702, 1784
BstV2I GAAGAC 1 cut(s) 675
BstX2I RGATCY 2 cut(s) 339, 2152
BstXI CCANNNNNNTGG 1 cut(s) 1896
BstYI RGATCY 2 cut(s) 339, 2152
BstZ17I GTATAC 1 cut(s) 1902
Bsu15I ATCGAT 1 cut(s) 2396
BsuI GTATCC 3 cut(s) 395, 1058, 1808
BsuRI GGCC 5 cut(s) 362, 753, 764, 1464, 2025
BsuTUI ATCGAT 1 cut(s) 2396
BtgI CCRYGG 1 cut(s) 696
BtgZI GCGATG 1 cut(s) 1047
BtrI CACGTC 2 cut(s) 1023, 1057
BtsI GCAGTG 2 cut(s) 1167, 1708
BtsIMutI CAGTG 7 cut(s) 919, 1167, 1527, 1708, 1734, 2193, 2244
Cac8I GCNNGC 4 cut(s) 747, 751, 772, 1111
CfoI GCGC 4 cut(s) 473, 1651, 1977, 2324
Cfr13I GGNCC 8 cut(s) 55, 361, 631, 1283, 1418, 1463, 1627, 1754
Cfr9I CCCGGG 1 cut(s) 662
ClaI ATCGAT 1 cut(s) 2396
CseI GACGC 4 cut(s) 308, 412, 883, 2142
Csp6I GTAC 6 cut(s) 529, 571, 793, 871, 1015, 1100
CspCI CAANNNNNGTGG 4 cut(s) 895, 930, 1858, 1893
CviQI GTAC 6 cut(s) 529, 571, 793, 871, 1015, 1100
DdeI CTNAG 8 cut(s) 216, 849, 1071, 1512, 2027, 2115, 2205, 2318
DpnI GATC 9 cut(s) 201, 341, 548, 798, 966, 1572, 1866, 2154, 2395
DpnII GATC 9 cut(s) 199, 339, 546, 796, 964, 1570, 1864, 2152, 2393
DraIII CACNNNGTG 1 cut(s) 1057
EaeI YGGCCR 1 cut(s) 751
Eam1104I CTCTTC 3 cut(s) 420, 640, 1632
EarI CTCTTC 3 cut(s) 420, 640, 1632
Ecl136II GAGCTC 1 cut(s) 149
Eco130I CCWWGG 1 cut(s) 1776
Eco147I AGGCCT 1 cut(s) 764
Eco24I GRGCYC 1 cut(s) 151
Eco31I GGTCTC 2 cut(s) 37, 1653
Eco47I GGWCC 6 cut(s) 55, 631, 1283, 1418, 1627, 1754
Eco53kI GAGCTC 1 cut(s) 149
Eco88I CYCGRG 3 cut(s) 388, 662, 990
EcoICRI GAGCTC 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 631
EcoRI GAATTC 3 cut(s) 2119, 2165, 2345
EcoRII CCWGG 6 cut(s) 1531, 1675, 1819, 1842, 1873, 1969
EcoT14I CCWWGG 1 cut(s) 1776
EcoT38I GRGCYC 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 1776
Esp3I CGTCTC 4 cut(s) 319, 326, 455, 469
FaqI GGGAC 4 cut(s) 644, 935, 1016, 1404
FauNDI CATATG 1 cut(s) 730
FbaI TGATCA 2 cut(s) 1570, 1864
FblI GTMKAC 2 cut(s) 1901, 2107
Fnu4HI GCNGC 8 cut(s) 68, 504, 1076, 1166, 1178, 1332, 1716, 1773
FriOI GRGCYC 1 cut(s) 151
Fsp4HI GCNGC 8 cut(s) 68, 504, 1076, 1166, 1178, 1332, 1716, 1773
FspBI CTAG 7 cut(s) 48, 561, 860, 1325, 1502, 2130, 2156
GlaI GCGC 4 cut(s) 472, 1650, 1976, 2323
GluI GCNGC 8 cut(s) 68, 504, 1076, 1166, 1178, 1332, 1716, 1773
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 451
HaeII RGCGCY 2 cut(s) 474, 2325
HaeIII GGCC 5 cut(s) 362, 753, 764, 1464, 2025
HapII CCGG 3 cut(s) 663, 1079, 2325
HgaI GACGC 4 cut(s) 308, 412, 883, 2142
HhaI GCGC 4 cut(s) 473, 1651, 1977, 2324
Hin1I GRCGYC 2 cut(s) 423, 432
Hin6I GCGC 4 cut(s) 471, 1649, 1975, 2322
HinP1I GCGC 4 cut(s) 471, 1649, 1975, 2322
HincII GTYRAC 3 cut(s) 213, 355, 1762
HindII GTYRAC 3 cut(s) 213, 355, 1762
HpaII CCGG 3 cut(s) 663, 1079, 2325
HphI GGTGA 8 cut(s) 196, 734, 856, 1520, 1835, 1874, 2171, 2447
Hpy166II GTNNAC 7 cut(s) 213, 355, 571, 1123, 1762, 1902, 2108
Hpy188III TCNNGA 5 cut(s) 388, 800, 886, 1286, 2130
Hpy8I GTNNAC 7 cut(s) 213, 355, 571, 1123, 1762, 1902, 2108
Hpy99I CGWCG 9 cut(s) 428, 434, 437, 899, 1027, 1043, 1058, 1094, 1097
HpyAV CCTTC 2 cut(s) 1487, 2405
HpyCH4III ACNGT 7 cut(s) 29, 463, 1127, 1522, 1546, 2197, 2292
HpyCH4IV ACGT 4 cut(s) 432, 869, 1022, 1056
HpyF10VI GCNNNNNNNGC 8 cut(s) 477, 584, 1174, 1328, 1470, 1595, 1681, 1715
HpyF3I CTNAG 8 cut(s) 216, 849, 1071, 1512, 2027, 2115, 2205, 2318
HpySE526I ACGT 4 cut(s) 432, 869, 1022, 1056
Hsp92I GRCGYC 2 cut(s) 423, 432
HspAI GCGC 4 cut(s) 471, 1649, 1975, 2322
KflI GGGWCCC 1 cut(s) 631
Ksp22I TGATCA 2 cut(s) 1570, 1864
Kzo9I GATC 9 cut(s) 199, 339, 546, 796, 964, 1570, 1864, 2152, 2393
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 1 cut(s) 147
Lsp1109I GCAGC 8 cut(s) 54, 490, 1087, 1164, 1177, 1318, 1702, 1784
LweI GCATC 7 cut(s) 116, 529, 1024, 1053, 1075, 1809, 2242
MaeI CTAG 7 cut(s) 48, 561, 860, 1325, 1502, 2130, 2156
MaeII ACGT 4 cut(s) 432, 869, 1022, 1056
MaeIII GTNAC 6 cut(s) 233, 392, 817, 844, 1891, 2191
MalI GATC 9 cut(s) 201, 341, 548, 798, 966, 1572, 1866, 2154, 2395
MboI GATC 9 cut(s) 199, 339, 546, 796, 964, 1570, 1864, 2152, 2393
MflI RGATCY 2 cut(s) 339, 2152
MhlI GDGCHC 5 cut(s) 16, 151, 610, 1301, 2448
MlsI TGGCCA 1 cut(s) 753
MluCI AATT 9 cut(s) 21, 99, 1144, 1836, 2119, 2165, 2179, 2339, 2345
MluNI TGGCCA 1 cut(s) 753
MmeI TCCRAC 5 cut(s) 267, 387, 1005, 2061, 2278
Mox20I TGGCCA 1 cut(s) 753
MroXI GAANNNNTTC 2 cut(s) 1443, 2183
MscI TGGCCA 1 cut(s) 753
MseI TTAA 7 cut(s) 24, 98, 497, 834, 977, 1548, 2066
MslI CAYNNNNRTG 4 cut(s) 229, 1118, 1940, 2382
Msp20I TGGCCA 1 cut(s) 753
MspA1I CMGCKG 2 cut(s) 67, 503
MspI CCGG 3 cut(s) 663, 1079, 2325
MspR9I CCNGG 9 cut(s) 663, 664, 1533, 1677, 1821, 1844, 1875, 1971, 2326
MvaI CCWGG 6 cut(s) 1533, 1677, 1821, 1844, 1875, 1971
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 8 cut(s) 477, 584, 1174, 1328, 1470, 1595, 1681, 1715
NciI CCSGG 3 cut(s) 663, 664, 2326
NdeI CATATG 1 cut(s) 730
NdeII GATC 9 cut(s) 199, 339, 546, 796, 964, 1570, 1864, 2152, 2393
NmeAIII GCCGAG 2 cut(s) 338, 746
NmuCI GTSAC 4 cut(s) 233, 817, 844, 2191
PaeR7I CTCGAG 1 cut(s) 990
PceI AGGCCT 1 cut(s) 764
PciSI GCTCTTC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 3 cut(s) 365, 426, 441
PdmI GAANNNNTTC 2 cut(s) 1443, 2183
PfeI GAWTC 7 cut(s) 370, 534, 725, 1732, 1741, 1986, 2301
Pfl23II CGTACG 1 cut(s) 792
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 8 cut(s) 69, 505, 1077, 1167, 1179, 1333, 1717, 1774
Ple19I CGATCG 1 cut(s) 549
Ppu21I YACGTR 1 cut(s) 870
PpuMI RGGWCCY 1 cut(s) 631
PsiI TTATAA 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 151
Psp5II RGGWCCY 1 cut(s) 631
Psp6I CCWGG 6 cut(s) 1531, 1675, 1819, 1842, 1873, 1969
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 6 cut(s) 1531, 1675, 1819, 1842, 1873, 1969
PspLI CGTACG 1 cut(s) 792
PspPI GGNCC 8 cut(s) 55, 361, 631, 1283, 1418, 1463, 1627, 1754
PspPPI RGGWCCY 1 cut(s) 631
PspXI VCTCGAGB 1 cut(s) 990
PstI CTGCAG 2 cut(s) 1336, 1774
PsuI RGATCY 2 cut(s) 339, 2152
PsyI GACNNNGTC 1 cut(s) 311
PvuI CGATCG 1 cut(s) 549
PvuII CAGCTG 1 cut(s) 67
RsaI GTAC 6 cut(s) 530, 572, 794, 872, 1016, 1101
RsaNI GTAC 6 cut(s) 529, 571, 793, 871, 1015, 1100
RseI CAYNNNNRTG 4 cut(s) 229, 1118, 1940, 2382
SacI GAGCTC 1 cut(s) 151
SapI GCTCTTC 1 cut(s) 420
SaqAI TTAA 7 cut(s) 24, 98, 497, 834, 977, 1548, 2066
SatI GCNGC 8 cut(s) 68, 504, 1076, 1166, 1178, 1332, 1716, 1773
Sau3AI GATC 9 cut(s) 199, 339, 546, 796, 964, 1570, 1864, 2152, 2393
Sau96I GGNCC 8 cut(s) 55, 361, 631, 1283, 1418, 1463, 1627, 1754
ScrFI CCNGG 9 cut(s) 663, 664, 1533, 1677, 1821, 1844, 1875, 1971, 2326
SduI GDGCHC 5 cut(s) 16, 151, 610, 1301, 2448
SfaNI GCATC 7 cut(s) 116, 529, 1024, 1053, 1075, 1809, 2242
SfcI CTRYAG 2 cut(s) 1332, 1770
Sfr274I CTCGAG 1 cut(s) 990
SinI GGWCC 6 cut(s) 55, 631, 1283, 1418, 1627, 1754
SlaI CTCGAG 1 cut(s) 990
SmaI CCCGGG 1 cut(s) 664
SmiMI CAYNNNNRTG 4 cut(s) 229, 1118, 1940, 2382
SmlI CTYRAG 3 cut(s) 486, 758, 990
SmoI CTYRAG 3 cut(s) 486, 758, 990
Sse9I AATT 9 cut(s) 21, 99, 1144, 1836, 2119, 2165, 2179, 2339, 2345
SseBI AGGCCT 1 cut(s) 764
SsiI CCGC 2 cut(s) 501, 566
SspMI CTAG 7 cut(s) 48, 561, 860, 1325, 1502, 2130, 2156
SstI GAGCTC 1 cut(s) 151
StuI AGGCCT 1 cut(s) 764
StyD4I CCNGG 9 cut(s) 661, 662, 1531, 1675, 1819, 1842, 1873, 1969, 2324
StyI CCWWGG 1 cut(s) 1776
TaaI ACNGT 7 cut(s) 29, 463, 1127, 1522, 1546, 2197, 2292
TaiI ACGT 4 cut(s) 435, 872, 1025, 1059
TaqI TCGA 7 cut(s) 31, 131, 709, 897, 991, 1095, 2396
TaqII GACCGA 2 cut(s) 527, 1406
TasI AATT 9 cut(s) 21, 99, 1144, 1836, 2119, 2165, 2179, 2339, 2345
TatI WGTACW 2 cut(s) 570, 1099
TfiI GAWTC 7 cut(s) 370, 534, 725, 1732, 1741, 1986, 2301
Tru1I TTAA 7 cut(s) 24, 98, 497, 834, 977, 1548, 2066
Tru9I TTAA 7 cut(s) 24, 98, 497, 834, 977, 1548, 2066
TscAI CASTG 7 cut(s) 919, 1167, 1527, 1708, 1734, 2200, 2244
TseFI GTSAC 4 cut(s) 233, 817, 844, 2191
TseI GCWGC 8 cut(s) 67, 503, 1075, 1165, 1177, 1331, 1715, 1772
Tsp45I GTSAC 4 cut(s) 233, 817, 844, 2191
TspDTI ATGAA 7 cut(s) 152, 219, 498, 717, 2052, 2338, 2444
TspGWI ACGGA 1 cut(s) 2256
TspMI CCCGGG 1 cut(s) 662
TspRI CASTG 7 cut(s) 919, 1167, 1527, 1708, 1734, 2200, 2244
Tth111I GACNNNGTC 1 cut(s) 311
VpaK11BI GGWCC 6 cut(s) 55, 631, 1283, 1418, 1627, 1754
XapI RAATTY 5 cut(s) 2119, 2165, 2179, 2339, 2345
XbaI TCTAGA 1 cut(s) 2129
XhoI CTCGAG 1 cut(s) 990
XmaI CCCGGG 1 cut(s) 662
XmiI GTMKAC 2 cut(s) 1901, 2107
XmnI GAANNNNTTC 2 cut(s) 1443, 2183
XspI CTAG 7 cut(s) 48, 561, 860, 1325, 1502, 2130, 2156
ZraI GACGTC 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.