Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0014594

Physical Location & Seq

Genomic Coordinates Reverse (-)

22145176 .. 22150868

Gene Structure 5693 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1179900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2355 bp

ATGAAACTCCATTCGCACACTCCTGCTCCCTCAGCCCTTATCTTCTTCTTCACCACCACCCTTCTCCTCCTCCGCCCTACCGAGTCAACTCAGCTTCCATATTCCTGTGACTCGTCCCCACCCTCAACCAGCTCCTACCCTTTTTGCAAAACGACCCTCCCCATCTACAAAAGAGTCCGGGACCTCGTCTCCCGCCTCACATTGGACGAGAAAATCTCCCAACTCGTTAACTCAGCCCCTCCGATTCCCCGACTAGGCATCCCGAGTTACGAGTGGTGGTCCGAGGCTTTACATGGCGTCGCGGACGTCGGCAAAGGTATTAAACTTTACCCCACCATTAACAACGCCACAAGCTTCCCTCAAGTCATCCTCACCGCGGCTTCTTTCAACGACCATCTCTGGTACCGCATTGGCCAGGTGATTGGGACTGAAGCTCGAGCAGTGTACAATGCCGGACAAGCAACAGGGATGACATTTTGGGCACCAAATATCAACATTTTCAGAGACCCGAGATGGGGGAGAGGGCAAGAGACACCTGGCGAAGATCCAACGGTCGCCGGAAGATACGCGGTGTCGTATGTGAGAGGGGTTCAGGGCGACTCGTTTTTTGGAGGGAAGCATAAACTTGGTGCTGGTGGTCGTCTTCAAGCTTCAGCTTGCTGCAAGCATTTCACAGCTTATGATTTGGACAGTTGGAACAATGTCACTCGTTTTCGCTTCGATGCTCGTGTGACACAGCAAGACCTAGCAGACACATACCACCCTCCGTTCCAAAAATGCGTGCAGGAAGGCCAAGCCAGCGGCATAATGTGTGCCTACAACCGCGTCAATGGGGTTCCAAGCTGTGCAGATTATAATCTCCTCACCAAGGTTGCAAGAGGACAATGGGACTTTCATGGGTACATCACGTCGGACTGCGATGCTGTGTCCATCATCCACGATATGCAAGGATACGCTAAAAAGCCCGAGGATGCAGTCGCAGATGTCCTCAAAGCAGGCATGGATGTGAACTGTGGATCCTACTTGAAGGATCACATTAAATCAGCAGTGGAGCAGAAAAAGCTAAATGTATCAAAAATAGACAGAGCACTCCACAACCTCTTCTCAGTGAGGATGAGGCTTGGCCTGTTTGATGGAAATCCACTGAAACAACCCTATGGAACCATTGGTCCAGACCAAGTGTGCTCCAAACTGCACCAGGCTTTAGCCCTAGAAGCTGCTCAGGATGGAATAGTGCTTTTGAAAAACTCTGGTAGGCTTCTCCCACTCCCAAAAGCAAAAGCCATTTCATTGGCTGTCATTGGTCCCCTTGCTAATGCTTCAGAAGCACTTCTGGGAAACTACCATGGTCTTCCATGCAAATCCGTCACCCCATTAGAAGCACTTCAGGACTATGCTAAAAACATGATCTCTAACCCGGGCTGTGATTCGGTTCAGTGTCCCCAAGCTTCTTTTGATGAAGCTGTTGGAATGGCAAAAGAAGCAGATTATTTGGTTTTGATTGTGGGATTGGACCAAAGTCAAGAGAGAGAGGGACATGATAGATTGCACTTGGAATTGCCTGGGAAGCAGCAACAGCTAATTACTAGTGTGGCTAAAGTTGCTAAGAAACCTGTTGTTCTTGTGATTCTTAGTGGGGGTCCAGTTGACATTTCTTTTGCTAAATTGGACAAAAAAGTTGGAAGCATCTTGTGGGCTGGATATCCTGGTGAAGCAGGTGGAATTGCCCTTGCTGAAATCATCTTTGGTGATCACAACCCAGGTGGAAGATTGCCAATGGCTTGGTACACACAAGATTATGTTAAAATGCCAATGACGGATATGAGAATTAGGCCTGACCCAAAAACAGGCTACGTTGGTCGCACTTACAGATTCTATACAGGCAGAAAACTTTTTGACTTTGGTTATGGCCTCAGCTACTCAAACTATGTTTATGAGTTTGCCTCTGCAGAATCCCAGAACAAGCTCTACTTAAATGAATCATCAATTAGTCCCACAGTTAAGTCATCGGACTTCGGCAGGTACCAGTTAGTCCAAGACCTAGGTGAAGAATTCTGCGAGAAAAAGAAGTTACCGGTCAGAGTTGGGGTTCAAAATCATGGCGAGATGGCCGGGAAGCATCCGGTGCTGCTTTTTATGGGTGAGAAGAATCCAAAAAATGGAAGGCCAATGAAGCAGTTGGTTGGATTCCAGAGTGTGATATTGAATGCTGGGGAAAGAGCAGAAGTTGAATTTGTTTTGAGCCCTTGTGAGCACTTGAGCCATCCAAATGAGGAAGGTTTGATGGTGGTTGAAGAAGGTTCTTATTTCTTGGGTGTTGGAGATGTAGAGTACCCTCTTGATATCATAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005911 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0030054 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

785

Amino Acids

86.27

Weight (kDa)

7.27

Isoelectric Point (pI)

32.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	113 - 368	5.7e-33	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	411 - 640	4.7e-52	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	709 - 775	4.3e-12	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014594)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78060
fragaria_vesca	FvH4_5g37840
malus_domestica	MD08G1179900.v1.1 MD15G1365200.v1.1
prunus_persica	Prupe.1G513200_v2.0.a1
pyrus_communis	pycom08g15400 pycom15g32750
rosa_chinensis	RchiOBHm_Chr7g0239821
rosa_laevigata	RLG00000000728
rosa_multiflora	Rmu_sc0007193.1_g000008
rosa_roxburghii	Rroxscaffold_3G00220790
rosa_rugosa	Rorug07G0329500
rosa_samantha	Rh7AG483700 Rh7BG456700 Rh7CG501200 Rh7DG469400
rosa_wichuraiana	Rw7G040570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	855
AarI	CACCTGC	1 cut(s)	1706
AatII	GACGTC	1 cut(s)	309
Acc36I	ACCTGC	2 cut(s)	1706, 2010
Acc65I	GGTACC	2 cut(s)	402, 2022
AccB1I	GGYRCC	3 cut(s)	402, 481, 2022
AccB7I	CCANNNNNTGG	1 cut(s)	2159
AccII	CGCG	4 cut(s)	302, 377, 569, 825
AciI	CCGC	9 cut(s)	73, 193, 302, 375, 377, 406, 569, 801, 823
AclWI	GGATC	4 cut(s)	539, 1011, 1024, 1038
AcoI	YGGCCR	2 cut(s)	412, 2109
AcsI	RAATTY	2 cut(s)	2051, 2231
AcuI	CTGAAG	4 cut(s)	450, 636, 1305, 1370
AcyI	GRCGYC	2 cut(s)	297, 306
AfaI	GTAC	6 cut(s)	404, 446, 902, 1787, 2024, 2333
AfiI	CCNNNNNNNGG	8 cut(s)	202, 254, 376, 514, 515, 868, 1847, 2159
AgeI	ACCGGT	1 cut(s)	2074
AgsI	TTSAA	8 cut(s)	388, 647, 1027, 1243, 2093, 2206, 2231, 2294
AhlI	ACTAGT	1 cut(s)	1586
AjiI	CACGTC	1 cut(s)	909
AjnI	CCWGG	6 cut(s)	414, 535, 1197, 1561, 1705, 1759
AjuI	GAANNNNNNNTTGG	2 cut(s)	1728, 1760
AloI	GAACNNNNNNTCC	4 cut(s)	1001, 1033, 1153, 1185
Alw21I	GWGCWC	3 cut(s)	1090, 1187, 2256
Alw26I	GTCTC	3 cut(s)	193, 498, 524
AlwI	GGATC	4 cut(s)	539, 1011, 1024, 1038
Ama87I	CYCGRG	5 cut(s)	262, 435, 508, 965, 1417
AoxI	GGCC	7 cut(s)	412, 790, 1123, 1832, 1909, 2109, 2165
ApeKI	GCWGC	4 cut(s)	660, 1217, 1570, 2128
ApoI	RAATTY	2 cut(s)	2051, 2231
ArsI	GACNNNNNNTTYG	4 cut(s)	590, 622, 1640, 1672
AsiGI	ACCGGT	1 cut(s)	2074
Asp700I	GAANNNNTTC	2 cut(s)	1329, 1383
Asp718I	GGTACC	2 cut(s)	402, 2022
AspA2I	CCTAGG	1 cut(s)	2041
AspS9I	GGNCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
AsuC2I	CCSGG	4 cut(s)	179, 1418, 1419, 2113
AsuHPI	GGTGA	9 cut(s)	43, 364, 430, 856, 1360, 1721, 1760, 2057, 2153
AvaI	CYCGRG	5 cut(s)	262, 435, 508, 965, 1417
AvaII	GGWCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
AvrII	CCTAGG	1 cut(s)	2041
BaeGI	GKGCMC	1 cut(s)	484
BalI	TGGCCA	1 cut(s)	414
BamHI	GGATCC	1 cut(s)	1016
BanI	GGYRCC	3 cut(s)	402, 481, 2022
BanII	GRGCYC	1 cut(s)	2246
BauI	CACGAG	1 cut(s)	726
BbsI	GAAGAC	2 cut(s)	635, 1343
Bbv12I	GWGCWC	3 cut(s)	1090, 1187, 2256
BbvCI	CCTCAGC	2 cut(s)	31, 1913
BbvI	GCAGC	4 cut(s)	647, 1204, 1582, 2115
BccI	CCATC	9 cut(s)	170, 402, 507, 938, 1127, 1220, 2101, 2271, 2278
BciT130I	CCWGG	6 cut(s)	416, 537, 1199, 1563, 1707, 1761
BciVI	GTATCC	1 cut(s)	944
BclI	TGATCA	1 cut(s)	1750
BcnI	CCSGG	4 cut(s)	179, 1418, 1419, 2113
BcoDI	GTCTC	3 cut(s)	193, 498, 524
BcuI	ACTAGT	1 cut(s)	1586
BfaI	CTAG	5 cut(s)	254, 746, 1211, 1587, 2042
BfmI	CTRYAG	1 cut(s)	1947
BfuAI	ACCTGC	2 cut(s)	1706, 2010
BfuI	GTATCC	1 cut(s)	944
BisI	GCNGC	6 cut(s)	378, 661, 802, 1218, 1571, 2129
BlnI	CCTAGG	1 cut(s)	2041
BlsI	GCNGC	6 cut(s)	379, 662, 803, 1219, 1572, 2130
Bme18I	GGWCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
BmeT110I	CYCGRG	5 cut(s)	262, 435, 508, 965, 1417
BmgBI	CACGTC	1 cut(s)	909
BmgT120I	GGNCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
BmiI	GGNNCC	9 cut(s)	182, 404, 483, 837, 1018, 1162, 1306, 1641, 2024
BmsI	GCATC	6 cut(s)	267, 712, 910, 961, 1695, 2128
BoxI	GACNNNNGTC	1 cut(s)	1518
BpiI	GAAGAC	2 cut(s)	635, 1343
BplI	GAGNNNNNCTC	4 cut(s)	200, 232, 1928, 1960
Bpu10I	CCTNAGC	3 cut(s)	31, 1221, 1913
BpuEI	CTTGAG	2 cut(s)	345, 2278
BpuMI	CCSGG	4 cut(s)	179, 1418, 1419, 2113
BsaBI	GATNNNNATC	2 cut(s)	855, 1137
BsaHI	GRCGYC	2 cut(s)	297, 306
BsaI	GGTCTC	1 cut(s)	498
BsaJI	CCNNGG	9 cut(s)	282, 375, 867, 966, 1345, 1417, 1562, 1759, 2041
BsaWI	WCCGGW	2 cut(s)	2074, 2122
BsaXI	ACNNNNNCTCC	4 cut(s)	10, 40, 173, 203
Bsc4I	CCNNNNNNNGG	8 cut(s)	202, 254, 376, 514, 515, 868, 1847, 2159
Bse118I	RCCGGY	1 cut(s)	2074
Bse1I	ACTGG	2 cut(s)	1643, 2026
Bse8I	GATNNNNATC	2 cut(s)	855, 1137
BseBI	CCWGG	6 cut(s)	416, 537, 1199, 1563, 1707, 1761
BseDI	CCNNGG	9 cut(s)	282, 375, 867, 966, 1345, 1417, 1562, 1759, 2041
BseJI	GATNNNNATC	2 cut(s)	855, 1137
BseLI	CCNNNNNNNGG	8 cut(s)	202, 254, 376, 514, 515, 868, 1847, 2159
BseMII	CTCAG	6 cut(s)	45, 104, 246, 1119, 1235, 1927
BseNI	ACTGG	2 cut(s)	1643, 2026
BseRI	GAGGAG	3 cut(s)	56, 59, 851
BseSI	GKGCMC	1 cut(s)	484
BseXI	GCAGC	4 cut(s)	647, 1204, 1582, 2115
BseYI	CCCAGC	1 cut(s)	2210
BsgI	GTGCAG	3 cut(s)	803, 867, 1178
Bsh1236I	CGCG	4 cut(s)	302, 377, 569, 825
Bsh1285I	CGRYCG	1 cut(s)	555
BshFI	GGCC	7 cut(s)	414, 792, 1125, 1834, 1911, 2111, 2167
BshNI	GGYRCC	3 cut(s)	402, 481, 2022
BshTI	ACCGGT	1 cut(s)	2074
BsiEI	CGRYCG	1 cut(s)	555
BsiHKAI	GWGCWC	3 cut(s)	1090, 1187, 2256
BsiHKCI	CYCGRG	5 cut(s)	262, 435, 508, 965, 1417
BsiSI	CCGG	7 cut(s)	178, 453, 558, 1418, 2075, 2112, 2123
BslFI	GGGAC	8 cut(s)	100, 194, 439, 902, 1290, 1425, 1548, 1977
BslI	CCNNNNNNNGG	8 cut(s)	202, 254, 376, 514, 515, 868, 1847, 2159
BsmAI	GTCTC	3 cut(s)	193, 498, 524
BsmBI	CGTCTC	1 cut(s)	193
BsmFI	GGGAC	8 cut(s)	100, 194, 439, 902, 1290, 1425, 1548, 1977
BsmI	GAATGC	1 cut(s)	2212
BsnI	GGCC	7 cut(s)	414, 792, 1125, 1834, 1911, 2111, 2167
Bso31I	GGTCTC	1 cut(s)	498
BsoBI	CYCGRG	5 cut(s)	262, 435, 508, 965, 1417
Bsp1286I	GDGCHC	5 cut(s)	484, 1090, 1187, 2246, 2256
Bsp1407I	TGTACA	1 cut(s)	444
Bsp143I	GATC	5 cut(s)	544, 1016, 1030, 1407, 1750
Bsp19I	CCATGG	1 cut(s)	1345
BspACI	CCGC	9 cut(s)	73, 193, 302, 375, 377, 406, 569, 801, 823
BspANI	GGCC	7 cut(s)	414, 792, 1125, 1834, 1911, 2111, 2167
BspCNI	CTCAG	6 cut(s)	44, 103, 245, 1118, 1234, 1926
BspFNI	CGCG	4 cut(s)	302, 377, 569, 825
BspLI	GGNNCC	9 cut(s)	182, 404, 483, 837, 1018, 1162, 1306, 1641, 2024
BspMAI	CTGCAG	1 cut(s)	1951
BspMI	ACCTGC	2 cut(s)	1706, 2010
BspPI	GGATC	4 cut(s)	539, 1011, 1024, 1038
BspT107I	GGYRCC	3 cut(s)	402, 481, 2022
BspTNI	GGTCTC	1 cut(s)	498
BsrFI	RCCGGY	1 cut(s)	2074
BsrGI	TGTACA	1 cut(s)	444
BsrI	ACTGG	2 cut(s)	1643, 2026
BssAI	RCCGGY	1 cut(s)	2074
BssECI	CCNNGG	9 cut(s)	282, 375, 867, 966, 1345, 1417, 1562, 1759, 2041
BssMI	GATC	5 cut(s)	544, 1016, 1030, 1407, 1750
BssNI	GRCGYC	2 cut(s)	297, 306
BssSI	CACGAG	1 cut(s)	726
BssT1I	CCWWGG	3 cut(s)	867, 1345, 2041
Bst2BI	CACGAG	1 cut(s)	726
Bst2UI	CCWGG	6 cut(s)	416, 537, 1199, 1563, 1707, 1761
Bst4CI	ACNGT	4 cut(s)	553, 692, 1013, 1999
Bst6I	CTCTTC	1 cut(s)	1106
BstACI	GRCGYC	2 cut(s)	297, 306
BstAPI	GCANNNNNTGC	1 cut(s)	2125
BstAUI	TGTACA	1 cut(s)	444
BstC8I	GCNNGC	5 cut(s)	658, 665, 782, 799, 997
BstDEI	CTNAG	8 cut(s)	31, 90, 232, 1105, 1221, 1605, 1631, 1913
BstDSI	CCRYGG	2 cut(s)	375, 1345
BstENI	CCTNNNNNAGG	1 cut(s)	866
BstFNI	CGCG	4 cut(s)	302, 377, 569, 825
BstKTI	GATC	5 cut(s)	547, 1019, 1033, 1410, 1753
BstMAI	GTCTC	3 cut(s)	193, 498, 524
BstMBI	GATC	5 cut(s)	544, 1016, 1030, 1407, 1750
BstMCI	CGRYCG	1 cut(s)	555
BstNI	CCWGG	6 cut(s)	416, 537, 1199, 1563, 1707, 1761
BstPAI	GACNNNNGTC	1 cut(s)	1518
BstSFI	CTRYAG	1 cut(s)	1947
BstSLI	GKGCMC	1 cut(s)	484
BstUI	CGCG	4 cut(s)	302, 377, 569, 825
BstV1I	GCAGC	4 cut(s)	647, 1204, 1582, 2115
BstV2I	GAAGAC	2 cut(s)	635, 1343
BstX2I	RGATCY	2 cut(s)	544, 1016
BstXI	CCANNNNNNTGG	3 cut(s)	422, 1291, 1782
BstYI	RGATCY	2 cut(s)	544, 1016
BsuI	GTATCC	1 cut(s)	944
BsuRI	GGCC	7 cut(s)	414, 792, 1125, 1834, 1911, 2111, 2167
BtgI	CCRYGG	2 cut(s)	375, 1345
BtgZI	GCGATG	1 cut(s)	933
BtrI	CACGTC	1 cut(s)	909
BtsI	GCAGTG	2 cut(s)	447, 1053
BtsIMutI	CAGTG	5 cut(s)	447, 1053, 1113, 1142, 1442
BveI	ACCTGC	2 cut(s)	1706, 2010
Cac8I	GCNNGC	5 cut(s)	658, 665, 782, 799, 997
Cfr10I	RCCGGY	1 cut(s)	2074
Cfr13I	GGNCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
Cfr42I	CCGCGG	1 cut(s)	378
Cfr9I	CCCGGG	1 cut(s)	1417
CseI	GACGC	2 cut(s)	286, 814
Csp6I	GTAC	6 cut(s)	403, 445, 901, 1786, 2023, 2332
CspAI	ACCGGT	1 cut(s)	2074
CspCI	CAANNNNNGTGG	2 cut(s)	1744, 1779
CviAII	CATG	8 cut(s)	293, 896, 1000, 1346, 1356, 1405, 1538, 2099
CviQI	GTAC	6 cut(s)	403, 445, 901, 1786, 2023, 2332
DdeI	CTNAG	8 cut(s)	31, 90, 232, 1105, 1221, 1605, 1631, 1913
DpnI	GATC	5 cut(s)	546, 1018, 1032, 1409, 1752
DpnII	GATC	5 cut(s)	544, 1016, 1030, 1407, 1750
EaeI	YGGCCR	2 cut(s)	412, 2109
Eam1104I	CTCTTC	1 cut(s)	1106
EarI	CTCTTC	1 cut(s)	1106
EciI	GGCGGA	1 cut(s)	62
Eco130I	CCWWGG	3 cut(s)	867, 1345, 2041
Eco147I	AGGCCT	1 cut(s)	1834
Eco24I	GRGCYC	1 cut(s)	2246
Eco31I	GGTCTC	1 cut(s)	498
Eco32I	GATATC	2 cut(s)	1703, 2344
Eco47I	GGWCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
Eco57I	CTGAAG	4 cut(s)	450, 636, 1305, 1370
Eco88I	CYCGRG	5 cut(s)	262, 435, 508, 965, 1417
EcoNI	CCTNNNNNAGG	1 cut(s)	866
EcoO109I	RGGNCCY	1 cut(s)	181
EcoRI	GAATTC	1 cut(s)	2051
EcoRII	CCWGG	6 cut(s)	414, 535, 1197, 1561, 1705, 1759
EcoRV	GATATC	2 cut(s)	1703, 2344
EcoT14I	CCWWGG	3 cut(s)	867, 1345, 2041
EcoT38I	GRGCYC	1 cut(s)	2246
ErhI	CCWWGG	3 cut(s)	867, 1345, 2041
Esp3I	CGTCTC	1 cut(s)	193
FaeI	CATG	8 cut(s)	296, 899, 1003, 1349, 1359, 1408, 1541, 2102
FalI	AAGNNNNNCTT	2 cut(s)	1955, 1987
FaqI	GGGAC	8 cut(s)	100, 194, 439, 902, 1290, 1425, 1548, 1977
FatI	CATG	8 cut(s)	292, 895, 999, 1345, 1355, 1404, 1537, 2098
FauI	CCCGC	1 cut(s)	200
FbaI	TGATCA	1 cut(s)	1750
Fnu4HI	GCNGC	6 cut(s)	378, 661, 802, 1218, 1571, 2129
FriOI	GRGCYC	1 cut(s)	2246
Fsp4HI	GCNGC	6 cut(s)	378, 661, 802, 1218, 1571, 2129
FspBI	CTAG	5 cut(s)	254, 746, 1211, 1587, 2042
GluI	GCNGC	6 cut(s)	378, 661, 802, 1218, 1571, 2129
GsaI	CCCAGC	1 cut(s)	2214
HaeIII	GGCC	7 cut(s)	414, 792, 1125, 1834, 1911, 2111, 2167
HapII	CCGG	7 cut(s)	178, 453, 558, 1418, 2075, 2112, 2123
HgaI	GACGC	2 cut(s)	286, 814
Hin1I	GRCGYC	2 cut(s)	297, 306
Hin1II	CATG	8 cut(s)	296, 899, 1003, 1349, 1359, 1408, 1541, 2102
HincII	GTYRAC	3 cut(s)	87, 229, 1648
HindII	GTYRAC	3 cut(s)	87, 229, 1648
HindIII	AAGCTT	3 cut(s)	352, 648, 1446
HpaI	GTTAAC	1 cut(s)	229
HpaII	CCGG	7 cut(s)	178, 453, 558, 1418, 2075, 2112, 2123
HphI	GGTGA	9 cut(s)	43, 364, 430, 856, 1360, 1721, 1760, 2057, 2153
Hpy166II	GTNNAC	6 cut(s)	87, 229, 445, 1009, 1648, 1788
Hpy188I	TCNGA	8 cut(s)	243, 283, 503, 913, 1324, 2011, 2081, 2354
Hpy188III	TCNNGA	7 cut(s)	262, 1172, 1223, 1388, 1523, 2191, 2339
Hpy8I	GTNNAC	6 cut(s)	87, 229, 445, 1009, 1648, 1788
Hpy99I	CGWCG	3 cut(s)	302, 311, 913
HpyAV	CCTTC	6 cut(s)	71, 782, 1021, 2157, 2270, 2291
HpyCH4III	ACNGT	4 cut(s)	553, 692, 1013, 1999
HpyCH4IV	ACGT	3 cut(s)	306, 908, 1854
HpyF3I	CTNAG	8 cut(s)	31, 90, 232, 1105, 1221, 1605, 1631, 1913
HpySE526I	ACGT	3 cut(s)	306, 908, 1854
Hsp92I	GRCGYC	2 cut(s)	297, 306
Hsp92II	CATG	8 cut(s)	296, 899, 1003, 1349, 1359, 1408, 1541, 2102
KpnI	GGTACC	2 cut(s)	406, 2026
Ksp22I	TGATCA	1 cut(s)	1750
KspAI	GTTAAC	1 cut(s)	229
KspI	CCGCGG	1 cut(s)	378
Kzo9I	GATC	5 cut(s)	544, 1016, 1030, 1407, 1750
LmnI	GCTCC	4 cut(s)	31, 137, 1051, 1190
Lsp1109I	GCAGC	4 cut(s)	647, 1204, 1582, 2115
LweI	GCATC	6 cut(s)	267, 712, 910, 961, 1695, 2128
MaeI	CTAG	5 cut(s)	254, 746, 1211, 1587, 2042
MaeII	ACGT	3 cut(s)	306, 908, 1854
MaeIII	GTNAC	6 cut(s)	107, 266, 703, 730, 1366, 2070
MalI	GATC	5 cut(s)	546, 1018, 1032, 1409, 1752
MboI	GATC	5 cut(s)	544, 1016, 1030, 1407, 1750
MflI	RGATCY	2 cut(s)	544, 1016
MhlI	GDGCHC	5 cut(s)	484, 1090, 1187, 2246, 2256
MlsI	TGGCCA	1 cut(s)	414
MluCI	AATT	8 cut(s)	1556, 1581, 1664, 1722, 1827, 1986, 2051, 2231
MluNI	TGGCCA	1 cut(s)	414
MlyI	GAGTC	4 cut(s)	92, 104, 183, 593
MmeI	TCCRAC	7 cut(s)	572, 674, 891, 1447, 1660, 2164, 2299
Mox20I	TGGCCA	1 cut(s)	414
MroXI	GAANNNNTTC	2 cut(s)	1329, 1383
MscI	TGGCCA	1 cut(s)	414
MseI	TTAA	7 cut(s)	228, 321, 339, 1038, 1803, 1973, 2001
MslI	CAYNNNNRTG	2 cut(s)	1004, 2268
Msp20I	TGGCCA	1 cut(s)	414
MspA1I	CMGCKG	2 cut(s)	377, 801
MspI	CCGG	7 cut(s)	178, 453, 558, 1418, 2075, 2112, 2123
Mva1269I	GAATGC	1 cut(s)	2212
MvaI	CCWGG	6 cut(s)	416, 537, 1199, 1563, 1707, 1761
MvnI	CGCG	4 cut(s)	302, 377, 569, 825
NciI	CCSGG	4 cut(s)	179, 1418, 1419, 2113
NcoI	CCATGG	1 cut(s)	1345
NdeII	GATC	5 cut(s)	544, 1016, 1030, 1407, 1750
NlaIII	CATG	8 cut(s)	296, 899, 1003, 1349, 1359, 1408, 1541, 2102
NlaIV	GGNNCC	9 cut(s)	182, 404, 483, 837, 1018, 1162, 1306, 1641, 2024
NmuCI	GTSAC	4 cut(s)	107, 703, 730, 1366
PaeR7I	CTCGAG	1 cut(s)	435
PaqCI	CACCTGC	1 cut(s)	1706
PceI	AGGCCT	1 cut(s)	1834
PctI	GAATGC	1 cut(s)	2212
PdmI	GAANNNNTTC	2 cut(s)	1329, 1383
PfeI	GAWTC	8 cut(s)	244, 1427, 1627, 1872, 1952, 1979, 2149, 2187
PflFI	GACNNNGTC	1 cut(s)	185
PflMI	CCANNNNNTGG	1 cut(s)	2159
PfoI	TCCNGGA	1 cut(s)	177
PinAI	ACCGGT	1 cut(s)	2074
PkrI	GCNGC	6 cut(s)	379, 662, 803, 1219, 1572, 2130
PleI	GAGTC	4 cut(s)	91, 104, 182, 593
PpsI	GAGTC	4 cut(s)	91, 104, 182, 593
PpuMI	RGGWCCY	1 cut(s)	181
PshAI	GACNNNNGTC	1 cut(s)	1518
PsiI	TTATAA	1 cut(s)	855
Psp5II	RGGWCCY	1 cut(s)	181
Psp6I	CCWGG	6 cut(s)	414, 535, 1197, 1561, 1705, 1759
PspFI	CCCAGC	1 cut(s)	2210
PspGI	CCWGG	6 cut(s)	414, 535, 1197, 1561, 1705, 1759
PspN4I	GGNNCC	9 cut(s)	182, 404, 483, 837, 1018, 1162, 1306, 1641, 2024
PspPI	GGNCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
PspPPI	RGGWCCY	1 cut(s)	181
PspXI	VCTCGAGB	1 cut(s)	435
PsrI	GAACNNNNNNTAC	2 cut(s)	1952, 1984
PstI	CTGCAG	1 cut(s)	1951
PsuI	RGATCY	2 cut(s)	544, 1016
PsyI	GACNNNGTC	1 cut(s)	185
RsaI	GTAC	6 cut(s)	404, 446, 902, 1787, 2024, 2333
RsaNI	GTAC	6 cut(s)	403, 445, 901, 1786, 2023, 2332
RseI	CAYNNNNRTG	2 cut(s)	1004, 2268
SacII	CCGCGG	1 cut(s)	378
SaqAI	TTAA	7 cut(s)	228, 321, 339, 1038, 1803, 1973, 2001
SatI	GCNGC	6 cut(s)	378, 661, 802, 1218, 1571, 2129
Sau3AI	GATC	5 cut(s)	544, 1016, 1030, 1407, 1750
Sau96I	GGNCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
SchI	GAGTC	4 cut(s)	92, 104, 183, 593
SduI	GDGCHC	5 cut(s)	484, 1090, 1187, 2246, 2256
SfaNI	GCATC	6 cut(s)	267, 712, 910, 961, 1695, 2128
SfcI	CTRYAG	1 cut(s)	1947
Sfr274I	CTCGAG	1 cut(s)	435
Sfr303I	CCGCGG	1 cut(s)	378
SgrBI	CCGCGG	1 cut(s)	378
SinI	GGWCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
SlaI	CTCGAG	1 cut(s)	435
SmaI	CCCGGG	1 cut(s)	1419
SmiMI	CAYNNNNRTG	2 cut(s)	1004, 2268
SmlI	CTYRAG	3 cut(s)	360, 435, 2257
SmoI	CTYRAG	3 cut(s)	360, 435, 2257
SpeI	ACTAGT	1 cut(s)	1586
Sse9I	AATT	8 cut(s)	1556, 1581, 1664, 1722, 1827, 1986, 2051, 2231
SseBI	AGGCCT	1 cut(s)	1834
SsiI	CCGC	9 cut(s)	73, 193, 302, 375, 377, 406, 569, 801, 823
SspMI	CTAG	5 cut(s)	254, 746, 1211, 1587, 2042
StuI	AGGCCT	1 cut(s)	1834
StyI	CCWWGG	3 cut(s)	867, 1345, 2041
TaaI	ACNGT	4 cut(s)	553, 692, 1013, 1999
TaiI	ACGT	3 cut(s)	309, 911, 1857
TaqI	TCGA	2 cut(s)	436, 720
TasI	AATT	8 cut(s)	1556, 1581, 1664, 1722, 1827, 1986, 2051, 2231
TatI	WGTACW	1 cut(s)	444
TauI	GCSGC	2 cut(s)	380, 804
TfiI	GAWTC	8 cut(s)	244, 1427, 1627, 1872, 1952, 1979, 2149, 2187
Tru1I	TTAA	7 cut(s)	228, 321, 339, 1038, 1803, 1973, 2001
Tru9I	TTAA	7 cut(s)	228, 321, 339, 1038, 1803, 1973, 2001
TscAI	CASTG	5 cut(s)	447, 1053, 1113, 1149, 1442
TseFI	GTSAC	4 cut(s)	107, 703, 730, 1366
TseI	GCWGC	4 cut(s)	660, 1217, 1570, 2128
Tsp45I	GTSAC	4 cut(s)	107, 703, 730, 1366
TspDTI	ATGAA	6 cut(s)	17, 884, 1278, 1473, 1992, 2186
TspGWI	ACGGA	3 cut(s)	756, 1354, 1832
TspMI	CCCGGG	1 cut(s)	1417
TspRI	CASTG	5 cut(s)	447, 1053, 1113, 1149, 1442
Tth111I	GACNNNGTC	1 cut(s)	185
Van91I	CCANNNNNTGG	1 cut(s)	2159
VpaK11BI	GGWCC	6 cut(s)	181, 279, 1169, 1304, 1513, 1640
XagI	CCTNNNNNAGG	1 cut(s)	866
XapI	RAATTY	2 cut(s)	2051, 2231
XhoI	CTCGAG	1 cut(s)	435
XmaI	CCCGGG	1 cut(s)	1417
XmaJI	CCTAGG	1 cut(s)	2041
XmnI	GAANNNNTTC	2 cut(s)	1329, 1383
XspI	CTAG	5 cut(s)	254, 746, 1211, 1587, 2042
ZraI	GACGTC	1 cut(s)	307

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.