MD15G1365200.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014594
Physical Location & Seq
Reverse (-)
44221230 .. 44227725
6496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1365200.v1.1.491

Sequence Viewer

Length: 2364 bp
ATGAAACTCCATTCACACGCTCCTCCTCCCTCAGCCCTTATCTTCTTCTTCACCACCGCCCTCCTCCTCCTCCTCCTCCTCCACCCTACCGAGTCAACTCAGCCTCCATATTCCTGTGACTCGTCCCAACCCTCCACCAGCTCCTACCCTTTTTGCAAAACCACTCTCCCCATCAACCAGCGAGTCCAGGACCTCATCTCCCGCCTCACATTGGACGAGAAAATCTCCCAACTCGTCAACTCGGCCCCTCCGATTCCCCGACTCGGTATCCCGAGTTACGAGTGGTGGTCCGAGGCTTTACACGGCGTCGCCGACGTCGGCAAAGGCATTAAACTTTACCCCACCATTCACAACGCCACGAGCTTCCCTCAAGTCATCCTCACCGCCGCTTCTTTCAACGACCATCTCTGGTACCGCATTGGCCAGGTGATTGGGACTGAAGCTCGAGCGGTGTACAATGCGGGACAAGCAACAGGGATGACATTTTGGGCACCAAACATCAACATCTTCAGGGACCCGAGATGGGGGAGAGGGCAAGAGACCCCTGGCGAAGATCCAACGGTCACAGGGAGATACGCCGTGTCGTATGTGAGAGGGGTTCAAGGTGATTCCTTCTATGGCGGGAAGCATAAGCTTGGTGCCGGTGGACGTCTTCAAGCTTCAGCTTGCTGCAAGCATTTCACAGCTTACGATTTGGACAATTGGAACAATATCACTCGATTTGGCTTCGACGCTTGTGTGACACAGCAAGACCTAGCAGACACGTACCAGCCTCCGTTCCAAAAATGCGTGCAGGAAGGCCAAGCTAGTGGCATAATGTGTGCCTATAACCGCATCAATGGAGTTCCAAGCTGTGCAGATCACAATCTCCTCACCAAGGTTGCAAGAGGACAATGGGACTTTCATGGGTACATCACATCGGACTGCGATGCTGTCTCCATCATCTACGATGTGCAAGGATACGCTAAAAAGCCCGAGGATGCAGTCGTTGATGTGCTTAAAGCAGGCATGGATGTGAACTGTGGATCCTACTTGAAGGATCACACAAAATCAGCAGTGGACCAGAAAAAGCTTAAAGTGTCAAAAATAGACAGAGCACTCCACAACCTCTTCTCAGTGAGGATGAGGCTAGGCCTGTTTGATGGCAATCCACTCAACCAACCCTATGGCACCATTGGTCCAGACCAAGTGTGCTCCCAACTGCACCAGGCTCTAGCCCTAGAAGCTGCTCAAGATGGAATAGTGCTTTTGAAAAACTCTGGCAGGCTTCTCCCACTCCCAAAAGCAAAAGCCATTTCACTTGCTGTCATTGGTCCCCATGCCAATGCTTTAGAAGCACTTCTGGGAAACTACCATGGTCCTCCATGCAAATCCATCACCCCATTAGAAGCACTTCAAGGCTATGCTAAGAACATAAATTATCACCCGGGTTGTGATTCGGTTAAGTGTCCACAAGCTGCTTTTGATGAAGCTGCTGGAACAGCAAAAGAAGCAGATTATGTGGTTTTGATTGTGGGATTGGACCAAAGTCAAGAGAGAGAGGAACTTGATAGATTGCACTTGGGATTGCCTGGGCAGCAGCAGGAGCTAATTAGCAGTGTGGCTAAAGTTGCTAAGAAACCTGTTATTCTTGTGATTCTTAGTGGGGGTCCAGTTGACATTTCTTTTGCTAAACATAACAAAAATATTGGAAGCATCTTGTGGGCTGGATATCCAGGTGAAGCTGGTGGAATTGCCCTTGCTGAAATCATCTTTGGTGAACACAACCCAGGTGGAAGATTGCCAATGGCTTGGTACACAGAAGATTATGTCAAAATGCCAATGACAGACATGAGAATGAGGTGTGAACCCAAAACAGGCTATCCTGGTCGAACTTACAGATTCTACACAGGCAAAAAAGTTTTTGACTTTGGTTATGGCCTCAGCTACTCAAACTATGTTTATAAGTTTTCCTCTGCAGCATCCCAAAACAAGCTCTACTTAAATGAATCATCAATTAGTCCCACTGCTAAGTCATCGGACTCTGGGCGGTATCAGTTAGTCCAAGACCTAGGTGGAGAATTCTGCGAGCAAAAGAAGTTATCGGTCAGAGTGAGGGTTCACAATCACGGGGAGATGGTGGGCAAGCATCCGGTGCTGCTTTTTGTGGGTGAGAAAAAGCCCAAAAATGGAAGGCCAATGAAGCAATTGGTTGGATTCAAAAGTGTGATACTGAGTGCTGGGGAAAGAGCTGAAATTGAATTTGATTTGAACCCTTGTGAGCACTTTAGCCATGCAAATGAGGAAGGTTTGATGGCGGTTGAAGAAGGTTCTTATTTCTTGGGCGTAGGAGATGCAAAGTACCCTCTTGACATCGTAGTGTGA

Protein Analysis

788

Amino Acids

86.3

Weight (kDa)

7.3

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 116 - 371 1.8e-31 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 414 - 643 7.4e-52 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 712 - 778 1.3e-11 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014594)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78060
fragaria_vesca FvH4_5g37840
malus_domestica MD08G1179900.v1.1 MD15G1365200.v1.1
prunus_persica Prupe.1G513200_v2.0.a1
pyrus_communis pycom08g15400 pycom15g32750
rosa_chinensis RchiOBHm_Chr7g0239821
rosa_laevigata RLG00000000728
rosa_multiflora Rmu_sc0007193.1_g000008
rosa_roxburghii Rroxscaffold_3G00220790
rosa_rugosa Rorug07G0329500
rosa_samantha Rh7AG483700 Rh7BG456700 Rh7CG501200 Rh7DG469400
rosa_wichuraiana Rw7G040570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1944
AatII GACGTC 2 cut(s) 318, 652
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 4 cut(s) 411, 490, 638, 1169
AccBSI CCGCTC 1 cut(s) 449
AclWI GGATC 4 cut(s) 548, 1020, 1033, 1047
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 2 cut(s) 2060, 2240
AcuI CTGAAG 3 cut(s) 459, 493, 645
AcyI GRCGYC 3 cut(s) 306, 315, 649
AfaI GTAC 6 cut(s) 413, 455, 767, 911, 1796, 2342
AfiI CCNNNNNNNGG 7 cut(s) 211, 263, 523, 524, 877, 1856, 2168
AflIII ACRYGT 1 cut(s) 762
AjnI CCWGG 8 cut(s) 186, 423, 544, 1206, 1570, 1714, 1768, 1864
AjuI GAANNNNNNNTTGG 2 cut(s) 1737, 1769
AloI GAACNNNNNNTCC 2 cut(s) 1010, 1042
Alw21I GWGCWC 3 cut(s) 1099, 1196, 2265
Alw26I GTCTC 2 cut(s) 533, 940
AlwI GGATC 4 cut(s) 548, 1020, 1033, 1047
Ama87I CYCGRG 5 cut(s) 271, 444, 517, 974, 1426
AoxI GGCC 6 cut(s) 243, 421, 799, 1132, 1918, 2174
ApeKI GCWGC 8 cut(s) 669, 1226, 1457, 1472, 1576, 1579, 1958, 2137
ApoI RAATTY 2 cut(s) 2060, 2240
ArsI GACNNNNNNTTYG 2 cut(s) 1649, 1681
Asp700I GAANNNNTTC 2 cut(s) 1338, 1392
Asp718I GGTACC 1 cut(s) 411
AspA2I CCTAGG 1 cut(s) 2050
AsuC2I CCSGG 2 cut(s) 1427, 1428
AvaI CYCGRG 5 cut(s) 271, 444, 517, 974, 1426
AvaII GGWCC 9 cut(s) 190, 288, 514, 1060, 1178, 1313, 1358, 1522, 1649
AvrII CCTAGG 1 cut(s) 2050
BaeGI GKGCMC 1 cut(s) 493
BalI TGGCCA 1 cut(s) 423
BamHI GGATCC 1 cut(s) 1025
BanI GGYRCC 4 cut(s) 411, 490, 638, 1169
BauI CACGAG 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 3 cut(s) 1099, 1196, 2265
BbvCI CCTCAGC 2 cut(s) 31, 1922
BbvI GCAGC 8 cut(s) 656, 1213, 1444, 1459, 1588, 1591, 1970, 2124
BccI CCATC 9 cut(s) 179, 411, 516, 947, 1136, 1229, 1382, 2110, 2287
BceAI ACGGC 2 cut(s) 319, 563
BciT130I CCWGG 8 cut(s) 188, 425, 546, 1208, 1572, 1716, 1770, 1866
BciVI GTATCC 2 cut(s) 278, 953
BcnI CCSGG 2 cut(s) 1427, 1428
BcoDI GTCTC 2 cut(s) 533, 940
BfaI CTAG 6 cut(s) 755, 807, 1130, 1214, 1220, 2051
BfmI CTRYAG 1 cut(s) 1956
BfuI GTATCC 2 cut(s) 278, 953
BisI GCNGC 9 cut(s) 387, 670, 1227, 1458, 1473, 1577, 1580, 1959, 2138
BlnI CCTAGG 1 cut(s) 2050
BlsI GCNGC 9 cut(s) 388, 671, 1228, 1459, 1474, 1578, 1581, 1960, 2139
Bme18I GGWCC 9 cut(s) 190, 288, 514, 1060, 1178, 1313, 1358, 1522, 1649
BmeT110I CYCGRG 5 cut(s) 271, 444, 517, 974, 1426
BmsI GCATC 7 cut(s) 843, 919, 970, 1704, 1970, 2137, 2323
BoxI GACNNNNGTC 1 cut(s) 1527
BpiI GAAGAC 1 cut(s) 644
BplI GAGNNNNNCTC 4 cut(s) 209, 241, 352, 384
Bpu10I CCTNAGC 2 cut(s) 31, 1922
BpuEI CTTGAG 2 cut(s) 354, 1215
BpuMI CCSGG 2 cut(s) 1427, 1428
BsaAI YACGTR 1 cut(s) 765
BsaBI GATNNNNATC 2 cut(s) 864, 1146
BsaHI GRCGYC 3 cut(s) 306, 315, 649
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 9 cut(s) 291, 544, 876, 975, 1354, 1426, 1571, 1768, 2050
BsaWI WCCGGW 1 cut(s) 2131
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 182, 212
Bsc4I CCNNNNNNNGG 7 cut(s) 211, 263, 523, 524, 877, 1856, 2168
Bse118I RCCGGY 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 1652
Bse8I GATNNNNATC 2 cut(s) 864, 1146
BseBI CCWGG 8 cut(s) 188, 425, 546, 1208, 1572, 1716, 1770, 1866
BseDI CCNNGG 9 cut(s) 291, 544, 876, 975, 1354, 1426, 1571, 1768, 2050
BseGI GGATG 7 cut(s) 375, 483, 985, 1018, 1128, 1961, 2128
BseJI GATNNNNATC 2 cut(s) 864, 1146
BseLI CCNNNNNNNGG 7 cut(s) 211, 263, 523, 524, 877, 1856, 2168
BseMII CTCAG 5 cut(s) 45, 113, 1128, 1936, 2204
BseNI ACTGG 1 cut(s) 1652
BseRI GAGGAG 9 cut(s) 12, 15, 53, 56, 59, 62, 65, 68, 860
BseSI GKGCMC 1 cut(s) 493
BseXI GCAGC 8 cut(s) 656, 1213, 1444, 1459, 1588, 1591, 1970, 2124
BseYI CCCAGC 1 cut(s) 2219
BsgI GTGCAG 3 cut(s) 812, 876, 1187
BshFI GGCC 6 cut(s) 245, 423, 801, 1134, 1920, 2176
BshNI GGYRCC 4 cut(s) 411, 490, 638, 1169
BsiHKAI GWGCWC 3 cut(s) 1099, 1196, 2265
BsiHKCI CYCGRG 5 cut(s) 271, 444, 517, 974, 1426
BsiSI CCGG 3 cut(s) 642, 1427, 2132
BslFI GGGAC 7 cut(s) 109, 448, 477, 527, 911, 1299, 1986
BslI CCNNNNNNNGG 7 cut(s) 211, 263, 523, 524, 877, 1856, 2168
BsmAI GTCTC 2 cut(s) 533, 940
BsmFI GGGAC 7 cut(s) 109, 448, 477, 527, 911, 1299, 1986
BsnI GGCC 6 cut(s) 245, 423, 801, 1134, 1920, 2176
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 5 cut(s) 271, 444, 517, 974, 1426
Bsp1286I GDGCHC 4 cut(s) 493, 1099, 1196, 2265
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 4 cut(s) 553, 859, 1025, 1039
Bsp19I CCATGG 1 cut(s) 1354
BspANI GGCC 6 cut(s) 245, 423, 801, 1134, 1920, 2176
BspCNI CTCAG 5 cut(s) 44, 112, 1127, 1935, 2205
BspMAI CTGCAG 1 cut(s) 1960
BspPI GGATC 4 cut(s) 548, 1020, 1033, 1047
BspT107I GGYRCC 4 cut(s) 411, 490, 638, 1169
BspTNI GGTCTC 1 cut(s) 533
BsrBI CCGCTC 1 cut(s) 449
BsrFI RCCGGY 1 cut(s) 641
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 1 cut(s) 1652
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 9 cut(s) 291, 544, 876, 975, 1354, 1426, 1571, 1768, 2050
BssMI GATC 4 cut(s) 553, 859, 1025, 1039
BssNI GRCGYC 3 cut(s) 306, 315, 649
BssSI CACGAG 1 cut(s) 358
BssT1I CCWWGG 3 cut(s) 876, 1354, 2050
Bst2BI CACGAG 1 cut(s) 358
Bst2UI CCWGG 8 cut(s) 188, 425, 546, 1208, 1572, 1716, 1770, 1866
Bst4CI ACNGT 2 cut(s) 562, 1022
Bst6I CTCTTC 1 cut(s) 1115
BstACI GRCGYC 3 cut(s) 306, 315, 649
BstAPI GCANNNNNTGC 1 cut(s) 2134
BstAUI TGTACA 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 765
BstC8I GCNNGC 7 cut(s) 667, 674, 791, 1006, 1265, 2069, 2126
BstDEI CTNAG 9 cut(s) 31, 99, 1114, 1407, 1614, 1640, 1922, 2010, 2213
BstDSI CCRYGG 1 cut(s) 1354
BstENI CCTNNNNNAGG 1 cut(s) 875
BstF5I GGATG 7 cut(s) 375, 483, 985, 1018, 1128, 1961, 2128
BstKTI GATC 4 cut(s) 556, 862, 1028, 1042
BstMAI GTCTC 2 cut(s) 533, 940
BstMBI GATC 4 cut(s) 553, 859, 1025, 1039
BstNI CCWGG 8 cut(s) 188, 425, 546, 1208, 1572, 1716, 1770, 1866
BstPAI GACNNNNGTC 1 cut(s) 1527
BstSFI CTRYAG 1 cut(s) 1956
BstSLI GKGCMC 1 cut(s) 493
BstV1I GCAGC 8 cut(s) 656, 1213, 1444, 1459, 1588, 1591, 1970, 2124
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 2 cut(s) 553, 1025
BstXI CCANNNNNNTGG 4 cut(s) 431, 809, 1166, 1791
BstYI RGATCY 2 cut(s) 553, 1025
BsuI GTATCC 2 cut(s) 278, 953
BsuRI GGCC 6 cut(s) 245, 423, 801, 1134, 1920, 2176
BtgI CCRYGG 1 cut(s) 1354
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 7 cut(s) 375, 483, 985, 1018, 1128, 1961, 2128
BtsI GCAGTG 3 cut(s) 1062, 1603, 2004
BtsIMutI CAGTG 4 cut(s) 1062, 1122, 1603, 2004
Cac8I GCNNGC 7 cut(s) 667, 674, 791, 1006, 1265, 2069, 2126
Cfr10I RCCGGY 1 cut(s) 641
Cfr9I CCCGGG 1 cut(s) 1426
CseI GACGC 2 cut(s) 295, 740
Csp6I GTAC 6 cut(s) 412, 454, 766, 910, 1795, 2341
CspCI CAANNNNNGTGG 2 cut(s) 1753, 1788
CviAII CATG 7 cut(s) 905, 1009, 1319, 1355, 1365, 1831, 2273
CviQI GTAC 6 cut(s) 412, 454, 766, 910, 1795, 2341
DdeI CTNAG 9 cut(s) 31, 99, 1114, 1407, 1614, 1640, 1922, 2010, 2213
DpnI GATC 4 cut(s) 555, 861, 1027, 1041
DpnII GATC 4 cut(s) 553, 859, 1025, 1039
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 1115
EarI CTCTTC 1 cut(s) 1115
Eco130I CCWWGG 3 cut(s) 876, 1354, 2050
Eco147I AGGCCT 1 cut(s) 1134
Eco31I GGTCTC 1 cut(s) 533
Eco32I GATATC 1 cut(s) 1712
Eco47I GGWCC 9 cut(s) 190, 288, 514, 1060, 1178, 1313, 1358, 1522, 1649
Eco57I CTGAAG 3 cut(s) 459, 493, 645
Eco88I CYCGRG 5 cut(s) 271, 444, 517, 974, 1426
EcoNI CCTNNNNNAGG 1 cut(s) 875
EcoO109I RGGNCCY 2 cut(s) 190, 514
EcoRI GAATTC 1 cut(s) 2060
EcoRII CCWGG 8 cut(s) 186, 423, 544, 1206, 1570, 1714, 1768, 1864
EcoRV GATATC 1 cut(s) 1712
EcoT14I CCWWGG 3 cut(s) 876, 1354, 2050
ErhI CCWWGG 3 cut(s) 876, 1354, 2050
FaeI CATG 7 cut(s) 908, 1012, 1322, 1358, 1368, 1834, 2276
FalI AAGNNNNNCTT 2 cut(s) 1964, 1996
FaqI GGGAC 7 cut(s) 109, 448, 477, 527, 911, 1299, 1986
FatI CATG 7 cut(s) 904, 1008, 1318, 1354, 1364, 1830, 2272
FauI CCCGC 3 cut(s) 209, 454, 614
Fnu4HI GCNGC 9 cut(s) 387, 670, 1227, 1458, 1473, 1577, 1580, 1959, 2138
FokI GGATG 7 cut(s) 362, 490, 992, 1025, 1135, 1948, 2115
Fsp4HI GCNGC 9 cut(s) 387, 670, 1227, 1458, 1473, 1577, 1580, 1959, 2138
FspBI CTAG 6 cut(s) 755, 807, 1130, 1214, 1220, 2051
GluI GCNGC 9 cut(s) 387, 670, 1227, 1458, 1473, 1577, 1580, 1959, 2138
GsaI CCCAGC 1 cut(s) 2223
HaeIII GGCC 6 cut(s) 245, 423, 801, 1134, 1920, 2176
HapII CCGG 3 cut(s) 642, 1427, 2132
HgaI GACGC 2 cut(s) 295, 740
Hin1I GRCGYC 3 cut(s) 306, 315, 649
Hin1II CATG 7 cut(s) 908, 1012, 1322, 1358, 1368, 1834, 2276
HincII GTYRAC 3 cut(s) 96, 238, 1657
HindII GTYRAC 3 cut(s) 96, 238, 1657
HindIII AAGCTT 3 cut(s) 632, 657, 1070
HpaII CCGG 3 cut(s) 642, 1427, 2132
Hpy188I TCNGA 5 cut(s) 252, 292, 922, 2020, 2090
Hpy188III TCNNGA 5 cut(s) 271, 1181, 1232, 1532, 2348
Hpy99I CGWCG 4 cut(s) 311, 317, 320, 734
HpyAV CCTTC 6 cut(s) 622, 791, 1030, 2166, 2279, 2300
HpyCH4III ACNGT 2 cut(s) 562, 1022
HpyCH4IV ACGT 3 cut(s) 315, 649, 764
HpyF3I CTNAG 9 cut(s) 31, 99, 1114, 1407, 1614, 1640, 1922, 2010, 2213
HpySE526I ACGT 3 cut(s) 315, 649, 764
Hsp92I GRCGYC 3 cut(s) 306, 315, 649
Hsp92II CATG 7 cut(s) 908, 1012, 1322, 1358, 1368, 1834, 2276
KflI GGGWCCC 1 cut(s) 514
KpnI GGTACC 1 cut(s) 415
Kzo9I GATC 4 cut(s) 553, 859, 1025, 1039
LmnI GCTCC 4 cut(s) 25, 146, 1199, 1585
Lsp1109I GCAGC 8 cut(s) 656, 1213, 1444, 1459, 1588, 1591, 1970, 2124
LweI GCATC 7 cut(s) 843, 919, 970, 1704, 1970, 2137, 2323
MaeI CTAG 6 cut(s) 755, 807, 1130, 1214, 1220, 2051
MaeII ACGT 3 cut(s) 315, 649, 764
MaeIII GTNAC 4 cut(s) 116, 275, 562, 739
MalI GATC 4 cut(s) 555, 861, 1027, 1041
MbiI CCGCTC 1 cut(s) 449
MboI GATC 4 cut(s) 553, 859, 1025, 1039
MfeI CAATTG 2 cut(s) 700, 2186
MflI RGATCY 2 cut(s) 553, 1025
MhlI GDGCHC 4 cut(s) 493, 1099, 1196, 2265
MlsI TGGCCA 1 cut(s) 423
MluCI AATT 9 cut(s) 700, 1417, 1590, 1731, 1995, 2060, 2186, 2235, 2240
MluNI TGGCCA 1 cut(s) 423
MlyI GAGTC 5 cut(s) 101, 113, 192, 255, 2015
MmeI TCCRAC 2 cut(s) 581, 2173
Mox20I TGGCCA 1 cut(s) 423
MroXI GAANNNNTTC 2 cut(s) 1338, 1392
MscI TGGCCA 1 cut(s) 423
MseI TTAA 5 cut(s) 330, 999, 1074, 1443, 1982
MslI CAYNNNNRTG 5 cut(s) 1013, 1323, 1835, 2277, 2357
Msp20I TGGCCA 1 cut(s) 423
MspI CCGG 3 cut(s) 642, 1427, 2132
MunI CAATTG 2 cut(s) 700, 2186
MvaI CCWGG 8 cut(s) 188, 425, 546, 1208, 1572, 1716, 1770, 1866
NciI CCSGG 2 cut(s) 1427, 1428
NcoI CCATGG 1 cut(s) 1354
NdeII GATC 4 cut(s) 553, 859, 1025, 1039
NlaIII CATG 7 cut(s) 908, 1012, 1322, 1358, 1368, 1834, 2276
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 3 cut(s) 116, 562, 739
PaeR7I CTCGAG 1 cut(s) 444
PceI AGGCCT 1 cut(s) 1134
PcsI WCGNNNNNNNCGW 2 cut(s) 248, 309
PdmI GAANNNNTTC 2 cut(s) 1338, 1392
PfeI GAWTC 7 cut(s) 253, 608, 1436, 1636, 1881, 1988, 2196
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 9 cut(s) 388, 671, 1228, 1459, 1474, 1578, 1581, 1960, 2139
PleI GAGTC 5 cut(s) 100, 113, 191, 255, 2015
PpsI GAGTC 5 cut(s) 100, 113, 191, 255, 2015
Ppu21I YACGTR 1 cut(s) 765
PpuMI RGGWCCY 2 cut(s) 190, 514
PshAI GACNNNNGTC 1 cut(s) 1527
PsiI TTATAA 1 cut(s) 1944
Psp5II RGGWCCY 2 cut(s) 190, 514
Psp6I CCWGG 8 cut(s) 186, 423, 544, 1206, 1570, 1714, 1768, 1864
PspFI CCCAGC 1 cut(s) 2219
PspGI CCWGG 8 cut(s) 186, 423, 544, 1206, 1570, 1714, 1768, 1864
PspPPI RGGWCCY 2 cut(s) 190, 514
PspXI VCTCGAGB 1 cut(s) 444
PstI CTGCAG 1 cut(s) 1960
PsuI RGATCY 2 cut(s) 553, 1025
RsaI GTAC 6 cut(s) 413, 455, 767, 911, 1796, 2342
RsaNI GTAC 6 cut(s) 412, 454, 766, 910, 1795, 2341
RseI CAYNNNNRTG 5 cut(s) 1013, 1323, 1835, 2277, 2357
SaqAI TTAA 5 cut(s) 330, 999, 1074, 1443, 1982
SatI GCNGC 9 cut(s) 387, 670, 1227, 1458, 1473, 1577, 1580, 1959, 2138
Sau3AI GATC 4 cut(s) 553, 859, 1025, 1039
SchI GAGTC 5 cut(s) 101, 113, 192, 255, 2015
SduI GDGCHC 4 cut(s) 493, 1099, 1196, 2265
SfaNI GCATC 7 cut(s) 843, 919, 970, 1704, 1970, 2137, 2323
SfcI CTRYAG 1 cut(s) 1956
Sfr274I CTCGAG 1 cut(s) 444
SinI GGWCC 9 cut(s) 190, 288, 514, 1060, 1178, 1313, 1358, 1522, 1649
SlaI CTCGAG 1 cut(s) 444
SmaI CCCGGG 1 cut(s) 1428
SmiMI CAYNNNNRTG 5 cut(s) 1013, 1323, 1835, 2277, 2357
SmlI CTYRAG 3 cut(s) 369, 444, 1230
SmoI CTYRAG 3 cut(s) 369, 444, 1230
Sse9I AATT 9 cut(s) 700, 1417, 1590, 1731, 1995, 2060, 2186, 2235, 2240
SseBI AGGCCT 1 cut(s) 1134
SspI AATATT 1 cut(s) 1687
SspMI CTAG 6 cut(s) 755, 807, 1130, 1214, 1220, 2051
StuI AGGCCT 1 cut(s) 1134
StyI CCWWGG 3 cut(s) 876, 1354, 2050
TaaI ACNGT 2 cut(s) 562, 1022
TaiI ACGT 3 cut(s) 318, 652, 767
TaqI TCGA 4 cut(s) 445, 718, 729, 1870
TaqII GACCGA 1 cut(s) 2074
TasI AATT 9 cut(s) 700, 1417, 1590, 1731, 1995, 2060, 2186, 2235, 2240
TatI WGTACW 1 cut(s) 453
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 7 cut(s) 253, 608, 1436, 1636, 1881, 1988, 2196
Tru1I TTAA 5 cut(s) 330, 999, 1074, 1443, 1982
Tru9I TTAA 5 cut(s) 330, 999, 1074, 1443, 1982
TscAI CASTG 4 cut(s) 1062, 1122, 1603, 2011
TseFI GTSAC 3 cut(s) 116, 562, 739
TseI GCWGC 8 cut(s) 669, 1226, 1457, 1472, 1576, 1579, 1958, 2137
Tsp45I GTSAC 3 cut(s) 116, 562, 739
TspDTI ATGAA 5 cut(s) 17, 893, 1482, 2001, 2195
TspGWI ACGGA 1 cut(s) 765
TspMI CCCGGG 1 cut(s) 1426
TspRI CASTG 4 cut(s) 1062, 1122, 1603, 2011
VpaK11BI GGWCC 9 cut(s) 190, 288, 514, 1060, 1178, 1313, 1358, 1522, 1649
XagI CCTNNNNNAGG 1 cut(s) 875
XapI RAATTY 2 cut(s) 2060, 2240
XcmI CCANNNNNNNNNTGG 1 cut(s) 2051
XhoI CTCGAG 1 cut(s) 444
XmaI CCCGGG 1 cut(s) 1426
XmaJI CCTAGG 1 cut(s) 2050
XmnI GAANNNNTTC 2 cut(s) 1338, 1392
XspI CTAG 6 cut(s) 755, 807, 1130, 1214, 1220, 2051
ZraI GACGTC 2 cut(s) 316, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.