FvH4_5g38950

Transmembrane protein 50 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015086
Physical Location & Seq
Forward (+)
28773480 .. 28775182
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38950.t1

Sequence Viewer

Length: 408 bp
ATGGATTTCCCGGAGCTTTGGGCAATCTTCGGGCCAGGCGTCGCCGGCGCTGTGTTCGGCGCCGGATGGTGGTTCTGGGTCGACGCCGTGGTTTGCAGCTCCGTCACCGTCTCGTTCCTTCACTACCTTCCAGGGATTTTTGCTTCTTTGGCGGCTTTGATGTTCAATTGTGTTAAGAAGGACGACATTGACTACTCTCCTTATGAAGAGGGCGAGTGGAGGTTGAAGCTTTGGCTGTTCATTGCTTATGTTGTGTCCTTTGTATCATTGGCGGCATCAGTGGGTTTGCTAATACAAGATTCACTTGTGACAACCGGCCCTTCGGTTTGGACAGGAACTGCTGGTGTATTGCAAGCCGTTTTCGTGTTGGTCAGTGGGCTTATATATTGGACTTCTCACCCGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0220

Protein Analysis

136

Amino Acids

14.79

Weight (kDa)

4.42

Isoelectric Point (pI)

20.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0220 PF05255 14 - 133 4.3e-23 Uncharacterised protein family (UPF0220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36980 AT1G36980
fragaria_vesca FvH4_5g38950
malus_domestica MD15G1359200.v1.1
prunus_persica Prupe.1G506000_v2.0.a1 Prupe.1G506000_v2.0.a1
pyrus_communis pycom15g32150
rosa_chinensis RchiOBHm_Chr7g0240921
rosa_multiflora Rmu_sc0004821.1_g000025
rosa_roxburghii Rroxscaffold_3G00219420
rosa_rugosa Rorug07G0340100 Rorug07G0340200
rosa_samantha Rh7BG466300 Rh7CG512800 Rh7DG479600
rosa_wichuraiana Rw7G041840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 81
AciI CCGC 2 cut(s) 152, 272
AcyI GRCGYC 3 cut(s) 39, 60, 84
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 2 cut(s) 166, 226
AjnI CCWGG 2 cut(s) 34, 130
AluBI AGCT 3 cut(s) 16, 99, 229
AluI AGCT 3 cut(s) 16, 99, 229
Alw26I GTCTC 1 cut(s) 115
AlwNI CAGNNNCTG 1 cut(s) 338
AoxI GGCC 2 cut(s) 32, 316
ApeKI GCWGC 1 cut(s) 96
AspLEI GCGC 2 cut(s) 50, 62
AspS9I GGNCC 2 cut(s) 32, 317
AsuC2I CCSGG 2 cut(s) 11, 401
AsuHPI GGTGA 2 cut(s) 97, 389
BanI GGYRCC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 60
BceAI ACGGC 2 cut(s) 71, 341
BciT130I CCWGG 2 cut(s) 36, 132
BcnI CCSGG 2 cut(s) 11, 401
BcoDI GTCTC 1 cut(s) 115
BfoI RGCGCY 2 cut(s) 51, 63
BisI GCNGC 3 cut(s) 97, 153, 273
BlsI GCNGC 3 cut(s) 98, 154, 274
Bme1390I CCNGG 4 cut(s) 11, 36, 132, 401
BmgT120I GGNCC 2 cut(s) 32, 317
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 4 cut(s) 11, 36, 132, 401
BmsI GCATC 1 cut(s) 284
BpuMI CCSGG 2 cut(s) 11, 401
BsaHI GRCGYC 3 cut(s) 39, 60, 84
BsaJI CCNNGG 2 cut(s) 87, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse118I RCCGGY 2 cut(s) 44, 314
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 2 cut(s) 36, 132
BseDI CCNNGG 2 cut(s) 87, 131
BseGI GGATG 1 cut(s) 71
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMI GCAATG 1 cut(s) 240
BseXI GCAGC 1 cut(s) 108
BshFI GGCC 2 cut(s) 34, 318
BshNI GGYRCC 1 cut(s) 59
BsiSI CCGG 5 cut(s) 11, 45, 63, 315, 401
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 1 cut(s) 115
BsmBI CGTCTC 1 cut(s) 115
BsnI GGCC 2 cut(s) 34, 318
BspACI CCGC 2 cut(s) 152, 272
BspANI GGCC 2 cut(s) 34, 318
BspLI GGNNCC 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 240
BsrFI RCCGGY 2 cut(s) 44, 314
BssAI RCCGGY 2 cut(s) 44, 314
BssECI CCNNGG 2 cut(s) 87, 131
BssNI GRCGYC 3 cut(s) 39, 60, 84
Bst2UI CCWGG 2 cut(s) 36, 132
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 3 cut(s) 39, 60, 84
BstC8I GCNNGC 2 cut(s) 46, 354
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 71
BstH2I RGCGCY 2 cut(s) 51, 63
BstHHI GCGC 2 cut(s) 50, 62
BstMAI GTCTC 1 cut(s) 115
BstMWI GCNNNNNNNGC 2 cut(s) 45, 149
BstNI CCWGG 2 cut(s) 36, 132
BstSCI CCNGG 4 cut(s) 9, 34, 130, 399
BstV1I GCAGC 1 cut(s) 108
BsuRI GGCC 2 cut(s) 34, 318
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 71
BtsIMutI CAGTG 2 cut(s) 285, 379
Cac8I GCNNGC 2 cut(s) 46, 354
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 2 cut(s) 50, 62
Cfr10I RCCGGY 2 cut(s) 44, 314
Cfr13I GGNCC 2 cut(s) 32, 317
CseI GACGC 2 cut(s) 28, 92
CviJI RGCY 9 cut(s) 16, 34, 99, 155, 229, 235, 318, 356, 379
CviKI_1 RGCY 9 cut(s) 16, 34, 99, 155, 229, 235, 318, 356, 379
DinI GGCGCC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 34, 130
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
Esp3I CGTCTC 1 cut(s) 115
FaiI YATR 4 cut(s) 204, 249, 383, 385
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 3 cut(s) 97, 153, 273
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 3 cut(s) 97, 153, 273
GlaI GCGC 2 cut(s) 49, 61
GluI GCNGC 3 cut(s) 97, 153, 273
HaeII RGCGCY 2 cut(s) 51, 63
HaeIII GGCC 2 cut(s) 34, 318
HapII CCGG 5 cut(s) 11, 45, 63, 315, 401
HgaI GACGC 2 cut(s) 28, 92
HhaI GCGC 2 cut(s) 50, 62
Hin1I GRCGYC 3 cut(s) 39, 60, 84
Hin6I GCGC 2 cut(s) 48, 60
HinP1I GCGC 2 cut(s) 48, 60
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 1 cut(s) 299
HpaII CCGG 5 cut(s) 11, 45, 63, 315, 401
HphI GGTGA 2 cut(s) 97, 389
Hpy166II GTNNAC 1 cut(s) 82
Hpy8I GTNNAC 1 cut(s) 82
Hpy99I CGWCG 2 cut(s) 44, 86
HpyAV CCTTC 4 cut(s) 128, 137, 172, 330
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 2 cut(s) 96, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 149
Hsp92I GRCGYC 3 cut(s) 39, 60, 84
HspAI GCGC 2 cut(s) 48, 60
KasI GGCGCC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
LmnI GCTCC 2 cut(s) 13, 104
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 284
MaeIII GTNAC 2 cut(s) 103, 307
MboII GAAGA 2 cut(s) 19, 218
MfeI CAATTG 1 cut(s) 166
MluCI AATT 1 cut(s) 166
Mly113I GGCGCC 1 cut(s) 60
MnlI CCTC 2 cut(s) 202, 213
MreI CGCCGGCG 1 cut(s) 44
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 1 cut(s) 174
MspI CCGG 5 cut(s) 11, 45, 63, 315, 401
MspR9I CCNGG 4 cut(s) 11, 36, 132, 401
MunI CAATTG 1 cut(s) 166
MvaI CCWGG 2 cut(s) 36, 132
MwoI GCNNNNNNNGC 2 cut(s) 45, 149
NaeI GCCGGC 1 cut(s) 46
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 2 cut(s) 11, 401
NgoMIV GCCGGC 1 cut(s) 44
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 2 cut(s) 103, 307
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 1 cut(s) 299
PfoI TCCNGGA 1 cut(s) 9
PkrI GCNGC 3 cut(s) 98, 154, 274
PluTI GGCGCC 1 cut(s) 63
Psp6I CCWGG 2 cut(s) 34, 130
PspGI CCWGG 2 cut(s) 34, 130
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 2 cut(s) 32, 317
PstNI CAGNNNCTG 1 cut(s) 338
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 3 cut(s) 97, 153, 273
Sau96I GGNCC 2 cut(s) 32, 317
ScrFI CCNGG 4 cut(s) 11, 36, 132, 401
SetI ASST 5 cut(s) 18, 101, 129, 224, 231
SfaNI GCATC 1 cut(s) 284
SfoI GGCGCC 1 cut(s) 61
SgrAI CRCCGGYG 1 cut(s) 44
Sse9I AATT 1 cut(s) 166
SsiI CCGC 2 cut(s) 152, 272
SspDI GGCGCC 1 cut(s) 59
StyD4I CCNGG 4 cut(s) 9, 34, 130, 399
TaaI ACNGT 1 cut(s) 109
TaqI TCGA 1 cut(s) 81
TasI AATT 1 cut(s) 166
TauI GCSGC 2 cut(s) 155, 275
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 2 cut(s) 285, 379
TseFI GTSAC 2 cut(s) 103, 307
TseI GCWGC 1 cut(s) 96
Tsp45I GTSAC 2 cut(s) 103, 307
TspDTI ATGAA 2 cut(s) 219, 229
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 2 cut(s) 285, 379
XmiI GTMKAC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.