Rh7DG479600

Transmembrane protein 50 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0015086
Physical Location & Seq
Forward (+)
68465631 .. 68467493
1863 bp
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UTR
Exon/CDS
Intron
Rh7DG479600.1

Sequence Viewer

Length: 408 bp
ATGGATATGCCGGAGCTTTGGGCAATCTTTGGGCCAGGCTTCGCTGGCGCTGTGTTCGGCGCTGGGTGGTGGTTCTGGGTCGACGCCGTCGTTTGCAGCTCCGTCACGGTCTCATTCGTTCACTACCTCCCAGGTATTTTTGCTTCTTTGGCGGCTTTGATGTTCAATTGTGTGAAGAAGGACGACATTGATTACTCTCCTTACGAAGAGGGCGAGTGGAGGTTGAAGCTTTGGCTGTTCATTGCTTATGTTGTTTCCTTTGTATCGTTGGCGGCATCAGTGGGTTTACTAATACAAGATTCACTTGAGACTACTGGCCCTTCAGTGTGGACAGGAACTGCTGGTGTATTGCAAGCCGTTTTCGTGTTGGTCAGTGGACTTATATATTGGACTGCTCACTCGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0220

Protein Analysis

135

Amino Acids

14.81

Weight (kDa)

4.33

Isoelectric Point (pI)

23.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0220 PF05255 14 - 133 2.2e-22 Uncharacterised protein family (UPF0220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36980 AT1G36980
fragaria_vesca FvH4_5g38950
malus_domestica MD15G1359200.v1.1
prunus_persica Prupe.1G506000_v2.0.a1 Prupe.1G506000_v2.0.a1
pyrus_communis pycom15g32150
rosa_chinensis RchiOBHm_Chr7g0240921
rosa_multiflora Rmu_sc0004821.1_g000025
rosa_roxburghii Rroxscaffold_3G00219420
rosa_rugosa Rorug07G0340100 Rorug07G0340200
rosa_samantha Rh7BG466300 Rh7CG512800 Rh7DG479600
rosa_wichuraiana Rw7G041840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 81
AciI CCGC 2 cut(s) 152, 272
AcuI CTGAAG 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 84
AgsI TTSAA 2 cut(s) 166, 226
AjnI CCWGG 2 cut(s) 34, 130
AluBI AGCT 3 cut(s) 16, 99, 229
AluI AGCT 3 cut(s) 16, 99, 229
Alw26I GTCTC 2 cut(s) 115, 302
AlwNI CAGNNNCTG 1 cut(s) 338
AoxI GGCC 2 cut(s) 32, 316
ApeKI GCWGC 1 cut(s) 96
AspLEI GCGC 2 cut(s) 50, 62
AspS9I GGNCC 2 cut(s) 32, 317
BarI GAAGNNNNNNTAC 2 cut(s) 127, 159
BbvI GCAGC 1 cut(s) 108
BceAI ACGGC 2 cut(s) 71, 341
BciT130I CCWGG 2 cut(s) 36, 132
BcoDI GTCTC 2 cut(s) 115, 302
BfoI RGCGCY 2 cut(s) 51, 63
BisI GCNGC 3 cut(s) 97, 153, 273
BlsI GCNGC 3 cut(s) 98, 154, 274
Bme1390I CCNGG 2 cut(s) 36, 132
BmgT120I GGNCC 2 cut(s) 32, 317
BmrFI CCNGG 2 cut(s) 36, 132
BmsI GCATC 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 2 cut(s) 36, 132
BseDI CCNNGG 1 cut(s) 130
BseMI GCAATG 1 cut(s) 240
BseNI ACTGG 1 cut(s) 319
BseXI GCAGC 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 62
BshFI GGCC 2 cut(s) 34, 318
BsiSI CCGG 1 cut(s) 11
BsmAI GTCTC 2 cut(s) 115, 302
BsnI GGCC 2 cut(s) 34, 318
Bso31I GGTCTC 1 cut(s) 115
BspACI CCGC 2 cut(s) 152, 272
BspANI GGCC 2 cut(s) 34, 318
BspTNI GGTCTC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 240
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 130
BssNI GRCGYC 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 36, 132
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 46, 354
BstH2I RGCGCY 2 cut(s) 51, 63
BstHHI GCGC 2 cut(s) 50, 62
BstMAI GTCTC 2 cut(s) 115, 302
BstMWI GCNNNNNNNGC 2 cut(s) 45, 149
BstNI CCWGG 2 cut(s) 36, 132
BstSCI CCNGG 2 cut(s) 34, 130
BstV1I GCAGC 1 cut(s) 108
BsuRI GGCC 2 cut(s) 34, 318
BtsIMutI CAGTG 3 cut(s) 285, 330, 379
Cac8I GCNNGC 2 cut(s) 46, 354
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 2 cut(s) 50, 62
Cfr13I GGNCC 2 cut(s) 32, 317
CseI GACGC 1 cut(s) 92
CviJI RGCY 9 cut(s) 16, 34, 39, 99, 155, 229, 235, 318, 356
CviKI_1 RGCY 9 cut(s) 16, 34, 39, 99, 155, 229, 235, 318, 356
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 34, 130
FaiI YATR 4 cut(s) 8, 249, 383, 385
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 3 cut(s) 97, 153, 273
Fsp4HI GCNGC 3 cut(s) 97, 153, 273
GlaI GCGC 2 cut(s) 49, 61
GluI GCNGC 3 cut(s) 97, 153, 273
GsaI CCCAGC 1 cut(s) 66
HaeII RGCGCY 2 cut(s) 51, 63
HaeIII GGCC 2 cut(s) 34, 318
HapII CCGG 1 cut(s) 11
HgaI GACGC 1 cut(s) 92
HhaI GCGC 2 cut(s) 50, 62
Hin1I GRCGYC 1 cut(s) 84
Hin6I GCGC 2 cut(s) 48, 60
HinP1I GCGC 2 cut(s) 48, 60
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 1 cut(s) 299
HpaII CCGG 1 cut(s) 11
Hpy166II GTNNAC 5 cut(s) 82, 121, 287, 330, 377
Hpy8I GTNNAC 5 cut(s) 82, 121, 287, 330, 377
Hpy99I CGWCG 2 cut(s) 86, 92
HpyAV CCTTC 2 cut(s) 172, 330
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 2 cut(s) 96, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 149
Hsp92I GRCGYC 1 cut(s) 84
HspAI GCGC 2 cut(s) 48, 60
LmnI GCTCC 2 cut(s) 13, 104
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 103
MboII GAAGA 2 cut(s) 187, 218
MfeI CAATTG 1 cut(s) 166
MluCI AATT 1 cut(s) 166
MnlI CCTC 3 cut(s) 137, 202, 213
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 2 cut(s) 36, 132
MunI CAATTG 1 cut(s) 166
MvaI CCWGG 2 cut(s) 36, 132
MwoI GCNNNNNNNGC 2 cut(s) 45, 149
NmuCI GTSAC 1 cut(s) 103
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 210
PfeI GAWTC 1 cut(s) 299
PflFI GACNNNGTC 1 cut(s) 86
PkrI GCNGC 3 cut(s) 98, 154, 274
Psp6I CCWGG 2 cut(s) 34, 130
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 2 cut(s) 34, 130
PspPI GGNCC 2 cut(s) 32, 317
PstNI CAGNNNCTG 1 cut(s) 338
PsyI GACNNNGTC 1 cut(s) 86
SalI GTCGAC 1 cut(s) 80
SatI GCNGC 3 cut(s) 97, 153, 273
Sau96I GGNCC 2 cut(s) 32, 317
ScrFI CCNGG 2 cut(s) 36, 132
SetI ASST 6 cut(s) 18, 101, 129, 136, 224, 231
SfaNI GCATC 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 1 cut(s) 166
SsiI CCGC 2 cut(s) 152, 272
StyD4I CCNGG 2 cut(s) 34, 130
TaaI ACNGT 1 cut(s) 109
TaqI TCGA 1 cut(s) 81
TasI AATT 1 cut(s) 166
TauI GCSGC 2 cut(s) 155, 275
TfiI GAWTC 1 cut(s) 299
TscAI CASTG 3 cut(s) 285, 330, 379
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 1 cut(s) 96
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 1 cut(s) 229
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 3 cut(s) 285, 330, 379
Tth111I GACNNNGTC 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.