Rorug07G0340200

Transmembrane protein 50 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0015086
Physical Location & Seq
Reverse (-)
37459749 .. 37477524
17776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0340200.1

Sequence Viewer

Length: 2277 bp
ATGTGGAAGCTTAAGTTTGGAGACGAGGGTGCAAATAACCCATACTTGTTCAGCACGAACAACTTCCATGGGAGGCAGACATGGGAGTTCGACCCGGATGCCGGCACACCCCAAGAGCGAGCAGAGGTCGAAGAAGCTCGGCTAAGTTTTTTCAACAATCGTCACCAGGTCCAGGCTTGTAGTGACCTCCTCTGCCGAATGCAGCTGCTGGGAGAGAAAAAGTTCAAACAAACAATTCCCCAAGTAAAGGTGGCAGATGATGAGGACTGTACATATGAAAAGGCCACCGCGGCATTCAGAAGGGCAACTACCTTCTACTCTGCCTTGCAATCACCTCACGGCCATTGGCCTGCTGAAAATGCCGGCCCTATGTTTTTCATTCCCCCCTTTGTTATCTCGCTCTATATTACGGGGCATCTTAGTAAGGTGTTCTCTGCAGAGCATTATAAGGAAATGCTTAGGTATATATACTGTCATCAGAACGAGGACGGTGGGTGGGGATTACACATACAGGCCCCTAGTATGATGTTTTGTACAATTCTTAACTACATTTGCATGCGTCTACTTGGAGAAGGACCCGATGGTGGTTTATACAACGCTTGTGCAAGAGCACGCAAGTGGATTCTTGATCATGGTGGTATAACACACTCATCATCCTGGGGAAAGACTTATATGGCAATACTTGGTGTGTATGAGTGGGAGGGATGCAACCCTATGCCCCCAGAGTTTTGGATTTTCCCGTCTTTGTTCCCTTTGCATCCAGCAAAAATGAACTGCTACTGCCGCTTGACTTACTTGCCTATGTCGTACCTGTTTGGGAAAAGATTTGTTGGCCCCATCACACCTCTCATTCTACAGCTGAGAGAAGAAATGTACAATGAGCCATACAATGAAATTCAATGGGGCAAAGTACGCCATCTCTGTGCAAAGGAGGATAACTACTATCCCCATGGCAAGTTACAGTGTTTGATGTGGGATACTCTTCACACAGTTTATGAGCCTCTTCTTTCTTGCTGGCCCTTCAAAAAGTTGAGAGAGAAGGCTCTGCAAGAAACTATTGAACACATTTATTATGAAGATGAAAATAGTAGATACATAACAATTGGAGCTGGGGAAAAGCCATTAATGATGCTTGCTTGCTGGGTTGAAGATCCGAATGGAGAATCCTTTAAGAAGCATCTTGCAAGAGTTGCAGATTACATATGGGTGGCAGAAGATGGAATAACTACGCAGAGTTTTGGTAGCCAAGCGTGGGATGCTAGTCTTTCGGTTCAAGCTTTACTTGCTGGAAATCTCACGGATGAACTTGGACCTGTACTTAAGAAAGCACATGAATTCCTAAACAAATCTCAGATTAGGGATAATCCTTCTGGTGACTTCTTAGCCCGTTTCCGTCACATTTCTAAAGGTGGTTGGACTTTCTCTGACCGAGATCATGGATGGCAAGTTTCTGATTGTACTGCAGAGGGTTTGACGTGTTGCCTTTTGTTATCAACGATGCCGACGCAAATCGTGGGTGAACCAATTGAATCTGAGCGCCTGTATGATGCTGTCAATGTCATACTTTATCTACAAAATCCAAATGGTGGTGTATCAGCCTGGCTGCCAACAGGAGCTCCACAATGGTTGGAGTGGTTCAATCCAATAGAATTTTTCGAGGATGTCGTCATTGAATACGAGTATGTTGAGTGCACTTCATCTTCAATTCAAGCATTAGCTTTATTCAGGAAGTTATACCCCGGGCATAGAAAGAAACATATCGATGATTTCATCACAAGTGCAGCATGGTACCTTGAAGACATGCAGACACCTGATGGCTCATGGTATGGAAAATGGGGAATCTGCTTCATCTATGGAACATGGTTTGCAATTCGAGGGTTGAAAGCTGCTGGCAGGACTTACAAATATTGTGAGGCAATACGCAGAGGTGTTGAATTTTTACTTAAAATACAGAAAGATGATGGTGGTTGGGGAGAGAGCTATACTTCTTGTACAAACAAGATCTATACACCTCTTGAAGGAGAGCGATCAAACTTGGTGCAGACCGCAATGGGATTAATGGGATTAATTCATGGTGGACAGGCGGAGAGAGACATTACTCCCATTCACAGAGCTGCGAAGCTTTTGATTAATTCTCAAATGGAAAATGGAGATTTCCCCCAACAGGAATTGATGGGGGTTTTCAATGTTAATGCCATGTTACACTATGCAGCATACAGGAATATCTACCCAATTTGGGCTCTCGCAGAATATCGCTCCCTTGCCTCCTCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0220

Protein Analysis

758

Amino Acids

86.8

Weight (kDa)

5.81

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 101 - 401 4.2e-38 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 415 - 752 2.6e-46 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36980 AT1G36980
fragaria_vesca FvH4_5g38950
malus_domestica MD15G1359200.v1.1
prunus_persica Prupe.1G506000_v2.0.a1 Prupe.1G506000_v2.0.a1
pyrus_communis pycom15g32150
rosa_chinensis RchiOBHm_Chr7g0240921
rosa_multiflora Rmu_sc0004821.1_g000025
rosa_roxburghii Rroxscaffold_3G00219420
rosa_rugosa Rorug07G0340100 Rorug07G0340200
rosa_samantha Rh7BG466300 Rh7CG512800 Rh7DG479600
rosa_wichuraiana Rw7G041840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
Acc65I GGTACC 1 cut(s) 1788
AccB1I GGYRCC 1 cut(s) 1788
AccB7I CCANNNNNTGG 1 cut(s) 1586
AccI GTMKAC 1 cut(s) 562
AccII CGCG 1 cut(s) 290
AciI CCGC 5 cut(s) 288, 290, 784, 2046, 2084
AclWI GGATC 1 cut(s) 1145
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 4 cut(s) 894, 1334, 1649, 1934
AfaI GTAC 9 cut(s) 271, 535, 809, 875, 912, 1317, 1459, 1790, 1993
AfiI CCNNNNNNNGG 9 cut(s) 101, 172, 247, 584, 1252, 1586, 2018, 2164, 2236
AflII CTTAAG 2 cut(s) 11, 1319
AflIII ACRYGT 1 cut(s) 1475
AjiI CACGTC 1 cut(s) 1476
AjnI CCWGG 4 cut(s) 165, 171, 656, 1598
AleI CACNNNNGTG 1 cut(s) 616
Alw21I GWGCWC 3 cut(s) 613, 1618, 1694
Alw26I GTCTC 2 cut(s) 15, 2085
Alw44I GTGCAC 1 cut(s) 1690
AlwI GGATC 1 cut(s) 1145
AlwNI CAGNNNCTG 1 cut(s) 208
Ama87I CYCGRG 1 cut(s) 1739
AoxI GGCC 7 cut(s) 282, 340, 347, 364, 513, 832, 1016
ApaLI GTGCAC 1 cut(s) 1690
ApeKI GCWGC 7 cut(s) 202, 205, 1603, 1781, 1886, 2114, 2210
ApoI RAATTY 4 cut(s) 894, 1334, 1649, 1934
AseI ATTAAT 4 cut(s) 1124, 2057, 2066, 2130
Asp700I GAANNNNTTC 2 cut(s) 62, 221
Asp718I GGTACC 1 cut(s) 1788
AspLEI GCGC 1 cut(s) 1539
AspS9I GGNCC 7 cut(s) 169, 365, 514, 575, 833, 1017, 1310
AsuC2I CCSGG 3 cut(s) 95, 1740, 1741
AsuHPI GGTGA 4 cut(s) 155, 324, 1385, 1529
AvaI CYCGRG 1 cut(s) 1739
AvaII GGWCC 3 cut(s) 169, 575, 1310
BaeGI GKGCMC 1 cut(s) 1694
BanI GGYRCC 1 cut(s) 1788
BanII GRGCYC 2 cut(s) 1618, 2242
BarI GAAGNNNNNNTAC 2 cut(s) 292, 324
BbsI GAAGAC 1 cut(s) 1803
Bbv12I GWGCWC 3 cut(s) 613, 1618, 1694
BbvI GCAGC 7 cut(s) 192, 214, 1590, 1793, 1873, 2101, 2222
BccI CCATC 8 cut(s) 575, 845, 924, 1211, 1434, 1808, 1955, 2167
BceAI ACGGC 1 cut(s) 355
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 4 cut(s) 167, 173, 658, 1600
BciVI GTATCC 1 cut(s) 970
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 3 cut(s) 95, 1740, 1741
BcoDI GTCTC 2 cut(s) 15, 2085
BfaI CTAG 2 cut(s) 519, 1260
BfmI CTRYAG 3 cut(s) 435, 854, 1461
BfoI RGCGCY 1 cut(s) 1540
BfrI CTTAAG 2 cut(s) 11, 1319
BfuI GTATCC 1 cut(s) 970
BglI GCCNNNNNGGC 1 cut(s) 290
BglII AGATCT 1 cut(s) 2001
BisI GCNGC 9 cut(s) 203, 206, 291, 784, 1604, 1782, 1887, 2115, 2211
BlsI GCNGC 9 cut(s) 204, 207, 292, 785, 1605, 1783, 1888, 2116, 2212
Bme1390I CCNGG 7 cut(s) 95, 167, 173, 658, 1600, 1740, 1741
Bme18I GGWCC 3 cut(s) 169, 575, 1310
BmeT110I CYCGRG 1 cut(s) 1739
BmgBI CACGTC 1 cut(s) 1476
BmgT120I GGNCC 7 cut(s) 169, 365, 514, 575, 833, 1017, 1310
BmiI GGNNCC 4 cut(s) 516, 577, 835, 1790
BmrFI CCNGG 7 cut(s) 95, 167, 173, 658, 1600, 1740, 1741
BmsI GCATC 9 cut(s) 88, 424, 695, 766, 1119, 1186, 1246, 1488, 1537
BpiI GAAGAC 1 cut(s) 1803
Bpu10I CCTNAGC 1 cut(s) 458
BpuMI CCSGG 3 cut(s) 95, 1740, 1741
Bsa29I ATCGAT 1 cut(s) 1761
BsaJI CCNNGG 6 cut(s) 67, 288, 657, 949, 1738, 1739
BsaXI ACNNNNNCTCC 2 cut(s) 1600, 1630
Bsc4I CCNNNNNNNGG 9 cut(s) 101, 172, 247, 584, 1252, 1586, 2018, 2164, 2236
Bse118I RCCGGY 2 cut(s) 101, 362
Bse3DI GCAATG 1 cut(s) 2055
BseBI CCWGG 4 cut(s) 167, 173, 658, 1600
BseCI ATCGAT 1 cut(s) 1761
BseDI CCNNGG 6 cut(s) 67, 288, 657, 949, 1738, 1739
BseGI GGATG 8 cut(s) 103, 653, 710, 757, 1261, 1306, 1445, 1666
BseLI CCNNNNNNNGG 9 cut(s) 101, 172, 247, 584, 1252, 1586, 2018, 2164, 2236
BseMI GCAATG 1 cut(s) 2055
BseMII CTCAG 3 cut(s) 851, 1364, 1524
BseRI GAGGAG 2 cut(s) 179, 2257
BseSI GKGCMC 1 cut(s) 1694
BseXI GCAGC 7 cut(s) 192, 214, 1590, 1793, 1873, 2101, 2222
BseYI CCCAGC 3 cut(s) 208, 1109, 1140
BsgI GTGCAG 2 cut(s) 1800, 2060
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 7 cut(s) 284, 342, 349, 366, 515, 834, 1018
BshNI GGYRCC 1 cut(s) 1788
BshVI ATCGAT 1 cut(s) 1761
BsiHKAI GWGCWC 3 cut(s) 613, 1618, 1694
BsiHKCI CYCGRG 1 cut(s) 1739
BsiSI CCGG 4 cut(s) 95, 102, 363, 1740
BslI CCNNNNNNNGG 9 cut(s) 101, 172, 247, 584, 1252, 1586, 2018, 2164, 2236
BsmAI GTCTC 2 cut(s) 15, 2085
BsmBI CGTCTC 1 cut(s) 15
BsmI GAATGC 2 cut(s) 204, 293
BsnI GGCC 7 cut(s) 284, 342, 349, 366, 515, 834, 1018
BsoBI CYCGRG 1 cut(s) 1739
Bsp1286I GDGCHC 4 cut(s) 613, 1618, 1694, 2242
Bsp1407I TGTACA 4 cut(s) 269, 533, 873, 1991
Bsp143I GATC 5 cut(s) 628, 1150, 1432, 2001, 2027
Bsp19I CCATGG 2 cut(s) 67, 949
BspACI CCGC 5 cut(s) 288, 290, 784, 2046, 2084
BspANI GGCC 7 cut(s) 284, 342, 349, 366, 515, 834, 1018
BspCNI CTCAG 3 cut(s) 852, 1363, 1525
BspDI ATCGAT 1 cut(s) 1761
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 4 cut(s) 516, 577, 835, 1790
BspMAI CTGCAG 2 cut(s) 439, 1465
BspPI GGATC 1 cut(s) 1145
BspT107I GGYRCC 1 cut(s) 1788
BspTI CTTAAG 2 cut(s) 11, 1319
BsrDI GCAATG 1 cut(s) 2055
BsrFI RCCGGY 2 cut(s) 101, 362
BsrGI TGTACA 4 cut(s) 269, 533, 873, 1991
BssAI RCCGGY 2 cut(s) 101, 362
BssECI CCNNGG 6 cut(s) 67, 288, 657, 949, 1738, 1739
BssMI GATC 5 cut(s) 628, 1150, 1432, 2001, 2027
BssT1I CCWWGG 2 cut(s) 67, 949
Bst2UI CCWGG 4 cut(s) 167, 173, 658, 1600
Bst4CI ACNGT 5 cut(s) 269, 473, 491, 963, 991
Bst6I CTCTTC 2 cut(s) 987, 1008
BstAFI CTTAAG 2 cut(s) 11, 1319
BstAPI GCANNNNNTGC 1 cut(s) 1190
BstAUI TGTACA 4 cut(s) 269, 533, 873, 1991
BstDEI CTNAG 7 cut(s) 143, 419, 458, 860, 1350, 1381, 1533
BstDSI CCRYGG 3 cut(s) 67, 288, 949
BstENI CCTNNNNNAGG 1 cut(s) 2016
BstF5I GGATG 8 cut(s) 103, 653, 710, 757, 1261, 1306, 1445, 1666
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 1 cut(s) 1540
BstHHI GCGC 1 cut(s) 1539
BstKTI GATC 5 cut(s) 631, 1153, 1435, 2004, 2030
BstMAI GTCTC 2 cut(s) 15, 2085
BstMBI GATC 5 cut(s) 628, 1150, 1432, 2001, 2027
BstMWI GCNNNNNNNGC 7 cut(s) 290, 359, 783, 912, 1190, 1256, 1283
BstNI CCWGG 4 cut(s) 167, 173, 658, 1600
BstNSI RCATGY 2 cut(s) 559, 1804
BstSCI CCNGG 7 cut(s) 93, 165, 171, 656, 1598, 1738, 1739
BstSFI CTRYAG 3 cut(s) 435, 854, 1461
BstSLI GKGCMC 1 cut(s) 1694
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 7 cut(s) 192, 214, 1590, 1793, 1873, 2101, 2222
BstV2I GAAGAC 1 cut(s) 1803
BstX2I RGATCY 2 cut(s) 1150, 2001
BstXI CCANNNNNNTGG 1 cut(s) 729
BstYI RGATCY 2 cut(s) 1150, 2001
Bsu15I ATCGAT 1 cut(s) 1761
BsuI GTATCC 1 cut(s) 970
BsuRI GGCC 7 cut(s) 284, 342, 349, 366, 515, 834, 1018
BsuTUI ATCGAT 1 cut(s) 1761
BtgI CCRYGG 3 cut(s) 67, 288, 949
BtrI CACGTC 1 cut(s) 1476
BtsCI GGATG 8 cut(s) 103, 653, 710, 757, 1261, 1306, 1445, 1666
BtsIMutI CAGTG 1 cut(s) 968
CaiI CAGNNNCTG 1 cut(s) 208
CfoI GCGC 1 cut(s) 1539
Cfr10I RCCGGY 2 cut(s) 101, 362
Cfr13I GGNCC 7 cut(s) 169, 365, 514, 575, 833, 1017, 1310
Cfr42I CCGCGG 1 cut(s) 291
Cfr9I CCCGGG 1 cut(s) 1739
ClaI ATCGAT 1 cut(s) 1761
CseI GACGC 2 cut(s) 548, 1513
CsiI ACCWGGT 1 cut(s) 165
Csp6I GTAC 9 cut(s) 270, 534, 808, 874, 911, 1316, 1458, 1789, 1992
CspCI CAANNNNNGTGG 2 cut(s) 1608, 1643
CviQI GTAC 9 cut(s) 270, 534, 808, 874, 911, 1316, 1458, 1789, 1992
DdeI CTNAG 7 cut(s) 143, 419, 458, 860, 1350, 1381, 1533
DpnI GATC 5 cut(s) 630, 1152, 1434, 2003, 2029
DpnII GATC 5 cut(s) 628, 1150, 1432, 2001, 2027
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 2 cut(s) 987, 1008
EarI CTCTTC 2 cut(s) 987, 1008
EciI GGCGGA 1 cut(s) 2099
Ecl136II GAGCTC 1 cut(s) 1616
Eco130I CCWWGG 2 cut(s) 67, 949
Eco24I GRGCYC 2 cut(s) 1618, 2242
Eco47I GGWCC 3 cut(s) 169, 575, 1310
Eco53kI GAGCTC 1 cut(s) 1616
Eco88I CYCGRG 1 cut(s) 1739
EcoICRI GAGCTC 1 cut(s) 1616
EcoNI CCTNNNNNAGG 1 cut(s) 2016
EcoO109I RGGNCCY 2 cut(s) 514, 575
EcoRI GAATTC 1 cut(s) 1334
EcoRII CCWGG 4 cut(s) 165, 171, 656, 1598
EcoT14I CCWWGG 2 cut(s) 67, 949
EcoT38I GRGCYC 2 cut(s) 1618, 2242
ErhI CCWWGG 2 cut(s) 67, 949
Esp3I CGTCTC 1 cut(s) 15
FalI AAGNNNNNCTT 2 cut(s) 1266, 1298
FauNDI CATATG 2 cut(s) 274, 1202
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 1 cut(s) 562
Fnu4HI GCNGC 9 cut(s) 203, 206, 291, 784, 1604, 1782, 1887, 2115, 2211
FokI GGATG 8 cut(s) 110, 640, 717, 744, 1268, 1313, 1452, 1673
FriOI GRGCYC 2 cut(s) 1618, 2242
Fsp4HI GCNGC 9 cut(s) 203, 206, 291, 784, 1604, 1782, 1887, 2115, 2211
FspBI CTAG 2 cut(s) 519, 1260
GlaI GCGC 1 cut(s) 1538
GluI GCNGC 9 cut(s) 203, 206, 291, 784, 1604, 1782, 1887, 2115, 2211
GsaI CCCAGC 3 cut(s) 212, 1113, 1144
HaeII RGCGCY 1 cut(s) 1540
HaeIII GGCC 7 cut(s) 284, 342, 349, 366, 515, 834, 1018
HapII CCGG 4 cut(s) 95, 102, 363, 1740
HgaI GACGC 2 cut(s) 548, 1513
HhaI GCGC 1 cut(s) 1539
Hin6I GCGC 1 cut(s) 1537
HinP1I GCGC 1 cut(s) 1537
HindIII AAGCTT 3 cut(s) 8, 1275, 2120
HinfI GANTC 4 cut(s) 622, 1163, 1529, 1839
HpaII CCGG 4 cut(s) 95, 102, 363, 1740
HphI GGTGA 4 cut(s) 155, 324, 1385, 1529
Hpy166II GTNNAC 4 cut(s) 563, 1520, 1692, 2078
Hpy188I TCNGA 7 cut(s) 299, 480, 1155, 1353, 1426, 1453, 1534
Hpy188III TCNNGA 4 cut(s) 626, 1726, 2015, 2271
Hpy8I GTNNAC 4 cut(s) 563, 1520, 1692, 2078
Hpy99I CGWCG 1 cut(s) 1507
HpyAV CCTTC 7 cut(s) 294, 322, 566, 1030, 1033, 1377, 2012
HpyCH4III ACNGT 5 cut(s) 269, 473, 491, 963, 991
HpyCH4IV ACGT 1 cut(s) 1475
HpyF10VI GCNNNNNNNGC 7 cut(s) 290, 359, 783, 912, 1190, 1256, 1283
HpyF3I CTNAG 7 cut(s) 143, 419, 458, 860, 1350, 1381, 1533
HpySE526I ACGT 1 cut(s) 1475
HspAI GCGC 1 cut(s) 1537
KpnI GGTACC 1 cut(s) 1792
KroI GCCGGC 2 cut(s) 101, 362
KroNI GCCGGC 2 cut(s) 103, 364
Ksp22I TGATCA 1 cut(s) 628
KspI CCGCGG 1 cut(s) 291
Kzo9I GATC 5 cut(s) 628, 1150, 1432, 2001, 2027
LmnI GCTCC 4 cut(s) 1106, 1613, 1621, 2261
Lsp1109I GCAGC 7 cut(s) 192, 214, 1590, 1793, 1873, 2101, 2222
LweI GCATC 9 cut(s) 88, 424, 695, 766, 1119, 1186, 1246, 1488, 1537
MabI ACCWGGT 1 cut(s) 165
MaeI CTAG 2 cut(s) 519, 1260
MaeII ACGT 1 cut(s) 1475
MaeIII GTNAC 6 cut(s) 161, 182, 957, 1373, 1394, 2199
MalI GATC 5 cut(s) 630, 1152, 1434, 2003, 2029
MboI GATC 5 cut(s) 628, 1150, 1432, 2001, 2027
MboII GAAGA 9 cut(s) 143, 878, 974, 995, 1088, 1160, 1226, 1692, 1808
MfeI CAATTG 2 cut(s) 1101, 1524
MflI RGATCY 2 cut(s) 1150, 2001
MhlI GDGCHC 4 cut(s) 613, 1618, 1694, 2242
MmeI TCCRAC 2 cut(s) 1394, 1608
MroNI GCCGGC 2 cut(s) 101, 362
MroXI GAANNNNTTC 2 cut(s) 62, 221
MslI CAYNNNNRTG 5 cut(s) 554, 616, 921, 1205, 1761
MspA1I CMGCKG 3 cut(s) 205, 290, 859
MspCI CTTAAG 2 cut(s) 11, 1319
MspI CCGG 4 cut(s) 95, 102, 363, 1740
MspR9I CCNGG 7 cut(s) 95, 167, 173, 658, 1600, 1740, 1741
MunI CAATTG 2 cut(s) 1101, 1524
Mva1269I GAATGC 2 cut(s) 204, 293
MvaI CCWGG 4 cut(s) 167, 173, 658, 1600
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 7 cut(s) 290, 359, 783, 912, 1190, 1256, 1283
NaeI GCCGGC 2 cut(s) 103, 364
NciI CCSGG 3 cut(s) 95, 1740, 1741
NcoI CCATGG 2 cut(s) 67, 949
NdeI CATATG 2 cut(s) 274, 1202
NdeII GATC 5 cut(s) 628, 1150, 1432, 2001, 2027
NgoMIV GCCGGC 2 cut(s) 101, 362
NlaIV GGNNCC 4 cut(s) 516, 577, 835, 1790
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 4 cut(s) 161, 182, 1373, 1394
NspI RCATGY 2 cut(s) 559, 1804
OliI CACNNNNGTG 1 cut(s) 616
PaeI GCATGC 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 1662
PctI GAATGC 2 cut(s) 204, 293
PdiI GCCGGC 2 cut(s) 103, 364
PdmI GAANNNNTTC 2 cut(s) 62, 221
PfeI GAWTC 4 cut(s) 622, 1163, 1529, 1839
PflMI CCANNNNNTGG 1 cut(s) 1586
PkrI GCNGC 9 cut(s) 204, 207, 292, 785, 1605, 1783, 1888, 2116, 2212
PpuMI RGGWCCY 1 cut(s) 575
PshBI ATTAAT 4 cut(s) 1124, 2057, 2066, 2130
PsiI TTATAA 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 1618
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 4 cut(s) 165, 171, 656, 1598
PspFI CCCAGC 3 cut(s) 208, 1109, 1140
PspGI CCWGG 4 cut(s) 165, 171, 656, 1598
PspN4I GGNNCC 4 cut(s) 516, 577, 835, 1790
PspPI GGNCC 7 cut(s) 169, 365, 514, 575, 833, 1017, 1310
PspPPI RGGWCCY 1 cut(s) 575
PstI CTGCAG 2 cut(s) 439, 1465
PstNI CAGNNNCTG 1 cut(s) 208
PsuI RGATCY 2 cut(s) 1150, 2001
PvuII CAGCTG 2 cut(s) 205, 859
RsaI GTAC 9 cut(s) 271, 535, 809, 875, 912, 1317, 1459, 1790, 1993
RsaNI GTAC 9 cut(s) 270, 534, 808, 874, 911, 1316, 1458, 1789, 1992
RseI CAYNNNNRTG 5 cut(s) 554, 616, 921, 1205, 1761
SacI GAGCTC 1 cut(s) 1618
SacII CCGCGG 1 cut(s) 291
SatI GCNGC 9 cut(s) 203, 206, 291, 784, 1604, 1782, 1887, 2115, 2211
Sau3AI GATC 5 cut(s) 628, 1150, 1432, 2001, 2027
Sau96I GGNCC 7 cut(s) 169, 365, 514, 575, 833, 1017, 1310
ScrFI CCNGG 7 cut(s) 95, 167, 173, 658, 1600, 1740, 1741
SduI GDGCHC 4 cut(s) 613, 1618, 1694, 2242
SexAI ACCWGGT 1 cut(s) 165
SfaNI GCATC 9 cut(s) 88, 424, 695, 766, 1119, 1186, 1246, 1488, 1537
SfcI CTRYAG 3 cut(s) 435, 854, 1461
Sfr303I CCGCGG 1 cut(s) 291
SgrBI CCGCGG 1 cut(s) 291
SinI GGWCC 3 cut(s) 169, 575, 1310
SmaI CCCGGG 1 cut(s) 1741
SmiMI CAYNNNNRTG 5 cut(s) 554, 616, 921, 1205, 1761
SmlI CTYRAG 2 cut(s) 11, 1319
SmoI CTYRAG 2 cut(s) 11, 1319
SphI GCATGC 1 cut(s) 559
SsiI CCGC 5 cut(s) 288, 290, 784, 2046, 2084
SspI AATATT 1 cut(s) 1907
SspMI CTAG 2 cut(s) 519, 1260
SstI GAGCTC 1 cut(s) 1618
StyD4I CCNGG 7 cut(s) 93, 165, 171, 656, 1598, 1738, 1739
StyI CCWWGG 2 cut(s) 67, 949
TaaI ACNGT 5 cut(s) 269, 473, 491, 963, 991
TaiI ACGT 1 cut(s) 1478
TaqI TCGA 5 cut(s) 90, 129, 1656, 1761, 1873
TaqII GACCGA 1 cut(s) 1443
TatI WGTACW 6 cut(s) 269, 533, 873, 1315, 1457, 1991
TauI GCSGC 2 cut(s) 293, 786
TfiI GAWTC 4 cut(s) 622, 1163, 1529, 1839
TscAI CASTG 1 cut(s) 968
TseFI GTSAC 4 cut(s) 161, 182, 1373, 1394
TseI GCWGC 7 cut(s) 202, 205, 1603, 1781, 1886, 2114, 2210
Tsp45I GTSAC 4 cut(s) 161, 182, 1373, 1394
TspGWI ACGGA 2 cut(s) 1313, 1382
TspMI CCCGGG 1 cut(s) 1739
TspRI CASTG 1 cut(s) 968
Van91I CCANNNNNTGG 1 cut(s) 1586
Vha464I CTTAAG 2 cut(s) 11, 1319
VneI GTGCAC 1 cut(s) 1690
VpaK11BI GGWCC 3 cut(s) 169, 575, 1310
VspI ATTAAT 4 cut(s) 1124, 2057, 2066, 2130
XagI CCTNNNNNAGG 1 cut(s) 2016
XapI RAATTY 4 cut(s) 894, 1334, 1649, 1934
XceI RCATGY 2 cut(s) 559, 1804
XmaI CCCGGG 1 cut(s) 1739
XmiI GTMKAC 1 cut(s) 562
XmnI GAANNNNTTC 2 cut(s) 62, 221
XspI CTAG 2 cut(s) 519, 1260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.