FvH4_6g02440

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007822
Physical Location & Seq
Forward (+)
1403773 .. 1406663
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02440.t3

Sequence Viewer

Length: 1320 bp
ATGAGTCGACAAGAGAAAAAGAAGGAAAAAATGTTGGAAAATGATGTCCATGCTGCATTGACCTGTGACGACATCCTAAATGAGATACTTCTTCGGCTGCCAGAGAAATTTGTTTTCAGATTGATACTTGTGTCAAAGAGGTGGCTTCGGGTGATATGTAGCTCTTCGTTTCGATCTGATTATCGTGCTAGATGGGGAGTGGACTTTCATCATCTGGGTTTCTTTGTCTGCAACCAGCTTTACCTCAACAGACCTAAAGATGGGATGCGCCGACCCAAGTCTGAGCCTGCACTTCAGTTGTTGTCAGCTTGTGAGGAAGGTGATGATGTATGCAATGATTTGATGGCTTCTGGGAATTGCAAACAGCTCGGTTATTTCATTGACTCTTGCAATGGGCTTCTTCTTTGTGGTCGCCACCCACTGACATATTCTGTTTGGAACCCAATCAGCAAAGTGCTATTCCAACTTCCTCAACCTCAGCGCTTCTACAAACGCTTGTGTATGGCATTCATCGCTGAGGATAATTTCGATGACATTCTTCAGTACAAGGTTATTCGTGCAAACTGTGAATGCAAACGCGAGGTTAACACTGTGTCCATTGAGACTTACTCTTCAGTGACTGGTACATGGAAGCATTTCACGCTCACTTGCTCTTCAAAATTTTCCTTGCGCCCCTGGACAGTGGCTACTGTGATCAATGGTGTCGTATACTGGTTTGCAACTCAGGGAAAGATAGCCATTAAGGAAAGCGTAGCGATTTATGACCCACGTGTTGGAAACACACGAGTAGAGTTGCTTAAATTACCTGATGGGAATATTTCGCAGGACTATGATGAGTTTGTTCTTGGGGAGTCCTCAGATGGGCTATTGCAGTATGGTCAGAGCAGCAAGTCAGGCATGGAGATATGGGTTCTTGAGAAGGAACAAGATAGTAGTATCTCATCTATTACCACAAACGTGCAATCAAACCGCAAGTGGAAAATTAGATACAGACTTGGTTTCAAAGCAATGTGGAAGAAAAATCCAAGCTTTTGCAAGCAGACAAAAGAAGCTCAATTACTGTCATTCCTTCCTCGGAATTCTGAATATGTCTTCATAAGATCTGGACAAAACATATTTCTCTTGCACATTGAAAGCCAAATGTTGAAGGTGAATCGATATCATGGTCCTAGCTCTCATATCCAGTGGGATTTCAGTAGAGTGGTGCCTTACTTTCAACCTTCTTGGCCACGATCTTCTTTATGTCATGAAAAGGCAGCAACATCTGCTTGGCCTTCTACAAACCAAGAAACTGCGGCACCTGCTTGGCCTTCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

440

Amino Acids

50.72

Weight (kDa)

9.0

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 118 - 334 3.2e-11 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 121 - 310 2.8e-15 F-box protein At3g26010-like, beta-propeller
Beta-prop_AT5G49610-like PF23635 127 - 385 1.1e-06 F-box protein AT5G49610-like, beta-propeller
FBA_1 PF07734 129 - 373 2e-13 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1309
Acc36I ACCTGC 1 cut(s) 1309
AccB1I GGYRCC 2 cut(s) 1204, 1297
AccB7I CCANNNNNTGG 1 cut(s) 773
AccI GTMKAC 2 cut(s) 7, 708
AccII CGCG 1 cut(s) 579
AciI CCGC 2 cut(s) 970, 1295
AcoI YGGCCR 1 cut(s) 1226
AcsI RAATTY 3 cut(s) 107, 659, 1078
AcuI CTGAAG 3 cut(s) 278, 524, 597
AcvI CACGTG 1 cut(s) 770
AfaI GTAC 2 cut(s) 545, 625
AfeI AGCGCT 1 cut(s) 482
AfiI CCNNNNNNNGG 3 cut(s) 260, 773, 861
AflIII ACRYGT 1 cut(s) 769
AgsI TTSAA 5 cut(s) 657, 1003, 1133, 1147, 1217
AjnI CCWGG 2 cut(s) 674, 1313
AleI CACNNNNGTG 1 cut(s) 956
AluBI AGCT 7 cut(s) 162, 238, 308, 367, 1029, 1052, 1173
AluI AGCT 7 cut(s) 162, 238, 308, 367, 1029, 1052, 1173
Alw26I GTCTC 1 cut(s) 596
AlwNI CAGNNNCTG 1 cut(s) 620
Aor51HI AGCGCT 1 cut(s) 482
AoxI GGCC 3 cut(s) 1226, 1271, 1307
ApeKI GCWGC 4 cut(s) 53, 97, 885, 1256
ApoI RAATTY 3 cut(s) 107, 659, 1078
Asp700I GAANNNNTTC 1 cut(s) 635
AspLEI GCGC 3 cut(s) 270, 483, 672
AspS9I GGNCC 1 cut(s) 1166
AsuHPI GGTGA 3 cut(s) 163, 332, 1162
AvaII GGWCC 1 cut(s) 1166
BaeI ACNNNNGTAYC 2 cut(s) 116, 149
BalI TGGCCA 1 cut(s) 1228
BanI GGYRCC 2 cut(s) 1204, 1297
BarI GAAGNNNNNNTAC 2 cut(s) 1041, 1073
BauI CACGAG 1 cut(s) 783
BbrPI CACGTG 1 cut(s) 770
BbsI GAAGAC 1 cut(s) 1084
BbvCI CCTCAGC 2 cut(s) 477, 516
BbvI GCAGC 4 cut(s) 40, 84, 897, 1268
BccI CCATC 5 cut(s) 186, 254, 337, 803, 854
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciT130I CCWGG 2 cut(s) 676, 1315
BclI TGATCA 1 cut(s) 693
BcoDI GTCTC 1 cut(s) 596
BfaI CTAG 2 cut(s) 189, 1170
BfoI RGCGCY 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 1309
BglII AGATCT 1 cut(s) 1100
BisI GCNGC 5 cut(s) 54, 98, 886, 1257, 1296
BlsI GCNGC 5 cut(s) 55, 99, 887, 1258, 1297
Bme1390I CCNGG 2 cut(s) 676, 1315
Bme18I GGWCC 1 cut(s) 1166
BmgT120I GGNCC 1 cut(s) 1166
BmiI GGNNCC 3 cut(s) 440, 1206, 1299
BmrFI CCNGG 2 cut(s) 676, 1315
BmsI GCATC 1 cut(s) 255
BoxI GACNNNNGTC 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 1084
BplI GAGNNNNNCTC 2 cut(s) 593, 625
Bpu10I CCTNAGC 2 cut(s) 477, 516
BpuEI CTTGAG 1 cut(s) 935
Bsa29I ATCGAT 1 cut(s) 1156
BsaAI YACGTR 1 cut(s) 770
BsaJI CCNNGG 2 cut(s) 674, 1073
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 773, 861
Bse1I ACTGG 3 cut(s) 625, 716, 1183
Bse3DI GCAATG 3 cut(s) 340, 397, 1014
BseBI CCWGG 2 cut(s) 676, 1315
BseCI ATCGAT 1 cut(s) 1156
BseDI CCNNGG 2 cut(s) 674, 1073
BseGI GGATG 2 cut(s) 72, 270
BseLI CCNNNNNNNGG 3 cut(s) 260, 773, 861
BseMI GCAATG 3 cut(s) 340, 397, 1014
BseMII CTCAG 5 cut(s) 273, 491, 507, 737, 870
BseNI ACTGG 3 cut(s) 625, 716, 1183
BseXI GCAGC 4 cut(s) 40, 84, 897, 1268
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 579
BshFI GGCC 3 cut(s) 1228, 1273, 1309
BshNI GGYRCC 2 cut(s) 1204, 1297
BshVI ATCGAT 1 cut(s) 1156
BslI CCNNNNNNNGG 3 cut(s) 260, 773, 861
BsmAI GTCTC 1 cut(s) 596
BsmI GAATGC 2 cut(s) 506, 575
BsnI GGCC 3 cut(s) 1228, 1273, 1309
Bsp143I GATC 4 cut(s) 173, 693, 1100, 1232
BspACI CCGC 2 cut(s) 970, 1295
BspANI GGCC 3 cut(s) 1228, 1273, 1309
BspCNI CTCAG 5 cut(s) 274, 490, 508, 736, 869
BspDI ATCGAT 1 cut(s) 1156
BspFNI CGCG 1 cut(s) 579
BspHI TCATGA 1 cut(s) 1246
BspLI GGNNCC 3 cut(s) 440, 1206, 1299
BspMI ACCTGC 1 cut(s) 1309
BspQI GCTCTTC 2 cut(s) 169, 658
BspT107I GGYRCC 2 cut(s) 1204, 1297
BsrDI GCAATG 3 cut(s) 340, 397, 1014
BsrI ACTGG 3 cut(s) 625, 716, 1183
BssECI CCNNGG 2 cut(s) 674, 1073
BssMI GATC 4 cut(s) 173, 693, 1100, 1232
BssNAI GTATAC 1 cut(s) 709
BssSI CACGAG 1 cut(s) 783
Bst1107I GTATAC 1 cut(s) 709
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 676, 1315
Bst4CI ACNGT 5 cut(s) 566, 592, 682, 691, 1062
Bst6I CTCTTC 3 cut(s) 169, 616, 658
BstAPI GCANNNNNTGC 1 cut(s) 1265
BstBAI YACGTR 1 cut(s) 770
BstC8I GCNNGC 2 cut(s) 288, 1037
BstDEI CTNAG 5 cut(s) 282, 477, 516, 723, 856
BstF5I GGATG 2 cut(s) 72, 270
BstFNI CGCG 1 cut(s) 579
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 3 cut(s) 270, 483, 672
BstKTI GATC 4 cut(s) 176, 696, 1103, 1235
BstMAI GTCTC 1 cut(s) 596
BstMBI GATC 4 cut(s) 173, 693, 1100, 1232
BstMWI GCNNNNNNNGC 5 cut(s) 512, 640, 894, 1265, 1301
BstNI CCWGG 2 cut(s) 676, 1315
BstPAI GACNNNNGTC 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 674, 1313
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 4 cut(s) 40, 84, 897, 1268
BstV2I GAAGAC 1 cut(s) 1084
BstX2I RGATCY 1 cut(s) 1100
BstYI RGATCY 1 cut(s) 1100
BstZ17I GTATAC 1 cut(s) 709
Bsu15I ATCGAT 1 cut(s) 1156
BsuRI GGCC 3 cut(s) 1228, 1273, 1309
BsuTUI ATCGAT 1 cut(s) 1156
BtgZI GCGATG 1 cut(s) 496
BtsCI GGATG 2 cut(s) 72, 270
BtsIMutI CAGTG 5 cut(s) 419, 588, 621, 687, 1190
BveI ACCTGC 1 cut(s) 1309
Cac8I GCNNGC 2 cut(s) 288, 1037
CaiI CAGNNNCTG 1 cut(s) 620
CciI TCATGA 1 cut(s) 1246
CfoI GCGC 3 cut(s) 270, 483, 672
Cfr13I GGNCC 1 cut(s) 1166
ClaI ATCGAT 1 cut(s) 1156
Csp6I GTAC 2 cut(s) 544, 624
CviAII CATG 5 cut(s) 50, 627, 898, 1163, 1247
CviQI GTAC 2 cut(s) 544, 624
DdeI CTNAG 5 cut(s) 282, 477, 516, 723, 856
DpnI GATC 4 cut(s) 175, 695, 1102, 1234
DpnII GATC 4 cut(s) 173, 693, 1100, 1232
EaeI YGGCCR 1 cut(s) 1226
Eam1104I CTCTTC 3 cut(s) 169, 616, 658
EarI CTCTTC 3 cut(s) 169, 616, 658
Eco32I GATATC 1 cut(s) 1160
Eco47I GGWCC 1 cut(s) 1166
Eco47III AGCGCT 1 cut(s) 482
Eco57I CTGAAG 3 cut(s) 278, 524, 597
Eco72I CACGTG 1 cut(s) 770
EcoRI GAATTC 1 cut(s) 1078
EcoRII CCWGG 2 cut(s) 674, 1313
EcoRV GATATC 1 cut(s) 1160
FaeI CATG 5 cut(s) 53, 630, 901, 1166, 1250
FatI CATG 5 cut(s) 49, 626, 897, 1162, 1246
FbaI TGATCA 1 cut(s) 693
FblI GTMKAC 2 cut(s) 7, 708
Fnu4HI GCNGC 5 cut(s) 54, 98, 886, 1257, 1296
FokI GGATG 2 cut(s) 59, 277
Fsp4HI GCNGC 5 cut(s) 54, 98, 886, 1257, 1296
FspBI CTAG 2 cut(s) 189, 1170
GlaI GCGC 3 cut(s) 269, 482, 671
GluI GCNGC 5 cut(s) 54, 98, 886, 1257, 1296
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 3 cut(s) 1228, 1273, 1309
HhaI GCGC 3 cut(s) 270, 483, 672
Hin1II CATG 5 cut(s) 53, 630, 901, 1166, 1250
Hin6I GCGC 3 cut(s) 268, 481, 670
HinP1I GCGC 3 cut(s) 268, 481, 670
HincII GTYRAC 2 cut(s) 8, 586
HindII GTYRAC 2 cut(s) 8, 586
HindIII AAGCTT 1 cut(s) 1027
HinfI GANTC 4 cut(s) 4, 383, 851, 1153
HpaI GTTAAC 1 cut(s) 586
HphI GGTGA 3 cut(s) 163, 332, 1162
Hpy166II GTNNAC 4 cut(s) 8, 202, 586, 709
Hpy188I TCNGA 7 cut(s) 119, 178, 283, 859, 882, 1077, 1084
Hpy188III TCNNGA 3 cut(s) 914, 1104, 1247
Hpy8I GTNNAC 4 cut(s) 8, 202, 586, 709
HpyAV CCTTC 8 cut(s) 16, 311, 913, 1079, 1141, 1230, 1284, 1320
HpyCH4III ACNGT 5 cut(s) 566, 592, 682, 691, 1062
HpyCH4IV ACGT 2 cut(s) 769, 957
HpyF10VI GCNNNNNNNGC 5 cut(s) 512, 640, 894, 1265, 1301
HpyF3I CTNAG 5 cut(s) 282, 477, 516, 723, 856
HpySE526I ACGT 2 cut(s) 769, 957
Hsp92II CATG 5 cut(s) 53, 630, 901, 1166, 1250
HspAI GCGC 3 cut(s) 268, 481, 670
Ksp22I TGATCA 1 cut(s) 693
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 4 cut(s) 173, 693, 1100, 1232
LguI GCTCTTC 2 cut(s) 169, 658
Lsp1109I GCAGC 4 cut(s) 40, 84, 897, 1268
LweI GCATC 1 cut(s) 255
MaeI CTAG 2 cut(s) 189, 1170
MaeII ACGT 2 cut(s) 769, 957
MaeIII GTNAC 2 cut(s) 65, 616
MalI GATC 4 cut(s) 175, 695, 1102, 1234
MboI GATC 4 cut(s) 173, 693, 1100, 1232
MboII GAAGA 9 cut(s) 83, 156, 392, 530, 603, 645, 1027, 1084, 1227
MflI RGATCY 1 cut(s) 1100
MlsI TGGCCA 1 cut(s) 1228
MluCI AATT 8 cut(s) 107, 355, 523, 659, 800, 981, 1055, 1078
MluNI TGGCCA 1 cut(s) 1228
MlyI GAGTC 3 cut(s) 13, 377, 860
MmeI TCCRAC 3 cut(s) 15, 487, 754
MnlI CCTC 9 cut(s) 132, 254, 307, 480, 486, 511, 574, 865, 1083
Mox20I TGGCCA 1 cut(s) 1228
MroXI GAANNNNTTC 1 cut(s) 635
MscI TGGCCA 1 cut(s) 1228
MseI TTAA 3 cut(s) 585, 741, 798
MslI CAYNNNNRTG 1 cut(s) 956
Msp20I TGGCCA 1 cut(s) 1228
MspR9I CCNGG 2 cut(s) 676, 1315
Mva1269I GAATGC 2 cut(s) 506, 575
MvaI CCWGG 2 cut(s) 676, 1315
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 5 cut(s) 512, 640, 894, 1265, 1301
NdeII GATC 4 cut(s) 173, 693, 1100, 1232
NlaIII CATG 5 cut(s) 53, 630, 901, 1166, 1250
NlaIV GGNNCC 3 cut(s) 440, 1206, 1299
NmuCI GTSAC 2 cut(s) 65, 616
OliI CACNNNNGTG 1 cut(s) 956
PagI TCATGA 1 cut(s) 1246
PaqCI CACCTGC 1 cut(s) 1309
PciSI GCTCTTC 2 cut(s) 169, 658
PctI GAATGC 2 cut(s) 506, 575
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 1 cut(s) 1153
PflMI CCANNNNNTGG 1 cut(s) 773
PkrI GCNGC 5 cut(s) 55, 99, 887, 1258, 1297
PleI GAGTC 3 cut(s) 12, 377, 859
PmaCI CACGTG 1 cut(s) 770
PmlI CACGTG 1 cut(s) 770
PpsI GAGTC 3 cut(s) 12, 377, 859
Ppu21I YACGTR 1 cut(s) 770
PshAI GACNNNNGTC 1 cut(s) 277
Psp6I CCWGG 2 cut(s) 674, 1313
PspCI CACGTG 1 cut(s) 770
PspGI CCWGG 2 cut(s) 674, 1313
PspN4I GGNNCC 3 cut(s) 440, 1206, 1299
PspPI GGNCC 1 cut(s) 1166
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 1100
RsaI GTAC 2 cut(s) 545, 625
RsaNI GTAC 2 cut(s) 544, 624
RseI CAYNNNNRTG 1 cut(s) 956
SalI GTCGAC 1 cut(s) 6
SapI GCTCTTC 2 cut(s) 169, 658
SaqAI TTAA 3 cut(s) 585, 741, 798
SatI GCNGC 5 cut(s) 54, 98, 886, 1257, 1296
Sau3AI GATC 4 cut(s) 173, 693, 1100, 1232
Sau96I GGNCC 1 cut(s) 1166
SchI GAGTC 3 cut(s) 13, 377, 860
ScrFI CCNGG 2 cut(s) 676, 1315
SfaNI GCATC 1 cut(s) 255
SinI GGWCC 1 cut(s) 1166
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
Sse9I AATT 8 cut(s) 107, 355, 523, 659, 800, 981, 1055, 1078
SsiI CCGC 2 cut(s) 970, 1295
SspI AATATT 1 cut(s) 817
SspMI CTAG 2 cut(s) 189, 1170
StyD4I CCNGG 2 cut(s) 674, 1313
TaaI ACNGT 5 cut(s) 566, 592, 682, 691, 1062
TaiI ACGT 2 cut(s) 772, 960
TaqI TCGA 4 cut(s) 7, 172, 528, 1156
TasI AATT 8 cut(s) 107, 355, 523, 659, 800, 981, 1055, 1078
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 1298
TfiI GAWTC 1 cut(s) 1153
Tru1I TTAA 3 cut(s) 585, 741, 798
Tru9I TTAA 3 cut(s) 585, 741, 798
TscAI CASTG 5 cut(s) 426, 595, 621, 687, 1190
TseFI GTSAC 2 cut(s) 65, 616
TseI GCWGC 4 cut(s) 53, 97, 885, 1256
Tsp45I GTSAC 2 cut(s) 65, 616
TspDTI ATGAA 5 cut(s) 197, 367, 499, 1084, 1263
TspRI CASTG 5 cut(s) 426, 595, 621, 687, 1190
Van91I CCANNNNNTGG 1 cut(s) 773
VpaK11BI GGWCC 1 cut(s) 1166
XapI RAATTY 3 cut(s) 107, 659, 1078
XmiI GTMKAC 2 cut(s) 7, 708
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 2 cut(s) 189, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.