MD12G1244700.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007822
Physical Location & Seq
Reverse (-)
31546115 .. 31548790
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1244700.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGACGGGTCAAAAGAAGTCTGCAAATGATGCTCATGAAGTATTCACCTGTGATGATGACATCCTACATGAGATCCTACTCCGTCTGCCAGAGAAATCTGTTTTCAGTTTAATACTCGTATCGAAGAGGTGGCTTCATGTGATATGTAGCCCTTCTTTTCGATGTGATTACCATGCTCGATGGAAAGTAAATTATCATCTTTTGGGCTTCTTTGTGTGCAACCATCTTTACCTCGGAATATCTAAATATGGCATGCGGCGGCCCCTAACAGAGCCCGCAATTCATTTTTTGTCAGCCTGTGAGGAAGGTGATAATCTAATGGTTTCTGCACCCAAACAGCTTGGTTACTTTCTTGGCACTTCCAATGGCCTACTTCTTTTTGGCCGCCATCCAATGACATATACGGTGTGGAACCCAGTCAGCAAACAGCTATGCAAACTTCCTCAACCTCAGCAGTTCTATCAAAGTTTGTGCATGGCATTCCTCACTGTCGAAGATGACTGTTTTAATGACGCAGTCCACTACAAGGTTATTCGGGCAAAATGTAACTGCAGACGGGAATCTAAAACTGTCTCCATTGAGACTTACTCTTCAATCAGCGGTACATGGAAGCATTTCACTCTCACTTGCTCTTCAACATTTGCATTGCGTCCTTGGAGAGAGGCTACTGTAGTTAATGGTATCCTACACTGGTTTGCAACCCAGGGAAACCTAGCCATTTATGATCCACGTCTTGGAAACAGGCGTATAAATCTGCTTAAATTACCAGAAGGGAGGATTTCTCAAGATTATGATGAGTGCGTTCTTGGGCATTCTTCAGATGGGCTTTTGCAGTATGGTCAGAGCAGTACGTCTGGCGTGGAAATATGGGTTCTCGAGGCCGGAGAAAGTAGTTTCTCATCGATCAGCACAGGCAATGTGCAGTCACAGAAGAAGTGGAATTTGAGGTACAGACTCGGTTTCAAGGCGATGTGGAAGCAGAATCCAGACCATGGAAAGCATTCGAAAGAAGCTAAACTTATGGCATTCCTTCATCAGAATTCCGAGTATATCTTCATAAGAGCTGGATGGAACATATATTTCTTCCACCTGAAAAGCCAGAAGCTTAAAGAGATTCGATATCTTGGTCGTGACCCTTCGATTCAATGGGATGACAGCACAGTGGTGCCTTTCTATAGACCGTCTTGGCCACGCTCTTCTTTATGTCCCTGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

406

Amino Acids

46.78

Weight (kDa)

9.05

Isoelectric Point (pI)

53.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 19 - 54 1.7e-06 F-box domain
FBA_3 PF08268 109 - 323 3.1e-06 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 116 - 281 3.7e-14 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 121 - 304 4.5e-11 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1165
AccB7I CCANNNNNTGG 2 cut(s) 734, 992
AciI CCGC 5 cut(s) 256, 259, 276, 385, 600
AclWI GGATC 2 cut(s) 67, 719
AcoI YGGCCR 2 cut(s) 382, 1187
AcsI RAATTY 2 cut(s) 940, 1039
AcuI CTGAAG 1 cut(s) 801
AfaI GTAC 3 cut(s) 604, 850, 950
AfiI CCNNNNNNNGG 3 cut(s) 526, 734, 992
AgsI TTSAA 4 cut(s) 594, 636, 964, 1145
AjiI CACGTC 1 cut(s) 731
AjnI CCWGG 2 cut(s) 702, 1208
AleI CACNNNNGTG 1 cut(s) 1163
AluBI AGCT 5 cut(s) 340, 430, 1013, 1064, 1105
AluI AGCT 5 cut(s) 340, 430, 1013, 1064, 1105
Alw26I GTCTC 2 cut(s) 575, 577
AlwI GGATC 2 cut(s) 67, 719
Ama87I CYCGRG 1 cut(s) 875
AoxI GGCC 5 cut(s) 260, 367, 382, 879, 1187
ApoI RAATTY 2 cut(s) 940, 1039
Asp700I GAANNNNTTC 2 cut(s) 614, 1000
AspS9I GGNCC 1 cut(s) 261
AsuHPI GGTGA 2 cut(s) 37, 320
AsuII TTCGAA 1 cut(s) 1004
AvaI CYCGRG 1 cut(s) 875
BalI TGGCCA 1 cut(s) 1189
BanI GGYRCC 1 cut(s) 1165
BanII GRGCYC 1 cut(s) 276
BbvCI CCTCAGC 1 cut(s) 450
BccI CCATC 5 cut(s) 174, 231, 396, 815, 1062
BciT130I CCWGG 2 cut(s) 704, 1210
BciVI GTATCC 1 cut(s) 692
BcoDI GTCTC 2 cut(s) 575, 577
BfaI CTAG 1 cut(s) 713
BfmI CTRYAG 3 cut(s) 550, 669, 1174
BfuI GTATCC 1 cut(s) 692
BisI GCNGC 3 cut(s) 257, 260, 385
BlsI GCNGC 3 cut(s) 258, 261, 386
Bme1390I CCNGG 2 cut(s) 704, 1210
BmeT110I CYCGRG 1 cut(s) 875
BmgBI CACGTC 1 cut(s) 731
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 3 cut(s) 263, 413, 1167
BmrFI CCNGG 2 cut(s) 704, 1210
BmrI ACTGGG 1 cut(s) 410
BmsI GCATC 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 410
BplI GAGNNNNNCTC 4 cut(s) 572, 604, 766, 798
Bpu10I CCTNAGC 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 1004
BpuEI CTTGAG 1 cut(s) 768
Bsa29I ATCGAT 1 cut(s) 902
BsaJI CCNNGG 6 cut(s) 232, 653, 702, 703, 991, 1208
Bsc4I CCNNNNNNNGG 3 cut(s) 526, 734, 992
Bse1I ACTGG 2 cut(s) 416, 695
Bse3DI GCAATG 2 cut(s) 644, 922
BseBI CCWGG 2 cut(s) 704, 1210
BseCI ATCGAT 1 cut(s) 902
BseDI CCNNGG 6 cut(s) 232, 653, 702, 703, 991, 1208
BseGI GGATG 5 cut(s) 60, 388, 1073, 1156, 1218
BseLI CCNNNNNNNGG 3 cut(s) 526, 734, 992
BseMI GCAATG 2 cut(s) 644, 922
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 2 cut(s) 416, 695
BsgI GTGCAG 2 cut(s) 312, 941
BshFI GGCC 5 cut(s) 262, 369, 384, 881, 1189
BshNI GGYRCC 1 cut(s) 1165
BshVI ATCGAT 1 cut(s) 902
BsiHKCI CYCGRG 1 cut(s) 875
BsiSI CCGG 1 cut(s) 882
BslFI GGGAC 1 cut(s) 1191
BslI CCNNNNNNNGG 3 cut(s) 526, 734, 992
BsmAI GTCTC 2 cut(s) 575, 577
BsmFI GGGAC 1 cut(s) 1191
BsmI GAATGC 4 cut(s) 479, 811, 1000, 1025
BsnI GGCC 5 cut(s) 262, 369, 384, 881, 1189
BsoBI CYCGRG 1 cut(s) 875
Bsp119I TTCGAA 1 cut(s) 1004
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 3 cut(s) 72, 724, 903
Bsp19I CCATGG 1 cut(s) 991
BspACI CCGC 5 cut(s) 256, 259, 276, 385, 600
BspANI GGCC 5 cut(s) 262, 369, 384, 881, 1189
BspCNI CTCAG 1 cut(s) 463
BspDI ATCGAT 1 cut(s) 902
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 3 cut(s) 263, 413, 1167
BspMAI CTGCAG 1 cut(s) 554
BspPI GGATC 2 cut(s) 67, 719
BspQI GCTCTTC 2 cut(s) 637, 1201
BspT104I TTCGAA 1 cut(s) 1004
BspT107I GGYRCC 1 cut(s) 1165
BsrDI GCAATG 2 cut(s) 644, 922
BsrI ACTGG 2 cut(s) 416, 695
BssECI CCNNGG 6 cut(s) 232, 653, 702, 703, 991, 1208
BssMI GATC 3 cut(s) 72, 724, 903
BssT1I CCWWGG 2 cut(s) 653, 991
Bst2UI CCWGG 2 cut(s) 704, 1210
Bst4CI ACNGT 7 cut(s) 406, 490, 503, 571, 670, 1162, 1182
Bst6I CTCTTC 4 cut(s) 119, 595, 637, 1201
BstAPI GCANNNNNTGC 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 1004
BstC8I GCNNGC 2 cut(s) 254, 276
BstDEI CTNAG 1 cut(s) 450
BstDSI CCRYGG 1 cut(s) 991
BstF5I GGATG 5 cut(s) 60, 388, 1073, 1156, 1218
BstKTI GATC 3 cut(s) 75, 727, 906
BstMAI GTCTC 2 cut(s) 575, 577
BstMBI GATC 3 cut(s) 72, 724, 903
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 2 cut(s) 704, 1210
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 2 cut(s) 702, 1208
BstSFI CTRYAG 3 cut(s) 550, 669, 1174
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
Bsu15I ATCGAT 1 cut(s) 902
BsuI GTATCC 1 cut(s) 692
BsuRI GGCC 5 cut(s) 262, 369, 384, 881, 1189
BsuTUI ATCGAT 1 cut(s) 902
BtgI CCRYGG 1 cut(s) 991
BtgZI GCGATG 1 cut(s) 983
BtrI CACGTC 1 cut(s) 731
BtsCI GGATG 5 cut(s) 60, 388, 1073, 1156, 1218
BtsIMutI CAGTG 3 cut(s) 486, 688, 1167
Cac8I GCNNGC 2 cut(s) 254, 276
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 261
ClaI ATCGAT 1 cut(s) 902
CseI GACGC 2 cut(s) 521, 638
Csp6I GTAC 3 cut(s) 603, 849, 949
CviAII CATG 8 cut(s) 35, 68, 137, 173, 253, 475, 606, 992
CviQI GTAC 3 cut(s) 603, 849, 949
DdeI CTNAG 1 cut(s) 450
DpnI GATC 3 cut(s) 74, 726, 905
DpnII GATC 3 cut(s) 72, 724, 903
EaeI YGGCCR 2 cut(s) 382, 1187
Eam1104I CTCTTC 4 cut(s) 119, 595, 637, 1201
EarI CTCTTC 4 cut(s) 119, 595, 637, 1201
Eco130I CCWWGG 2 cut(s) 653, 991
Eco24I GRGCYC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 1121
Eco57I CTGAAG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 875
EcoRI GAATTC 1 cut(s) 1039
EcoRII CCWGG 2 cut(s) 702, 1208
EcoRV GATATC 1 cut(s) 1121
EcoT14I CCWWGG 2 cut(s) 653, 991
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 2 cut(s) 653, 991
FaeI CATG 8 cut(s) 38, 71, 140, 176, 256, 478, 609, 995
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FaqI GGGAC 1 cut(s) 1191
FatI CATG 8 cut(s) 34, 67, 136, 172, 252, 474, 605, 991
FauI CCCGC 1 cut(s) 283
Fnu4HI GCNGC 3 cut(s) 257, 260, 385
FokI GGATG 4 cut(s) 47, 375, 1080, 1163
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 3 cut(s) 257, 260, 385
FspBI CTAG 1 cut(s) 713
GluI GCNGC 3 cut(s) 257, 260, 385
HaeIII GGCC 5 cut(s) 262, 369, 384, 881, 1189
HapII CCGG 1 cut(s) 882
HgaI GACGC 2 cut(s) 521, 638
Hin1II CATG 8 cut(s) 38, 71, 140, 176, 256, 478, 609, 995
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 5 cut(s) 560, 954, 982, 1114, 1141
HpaII CCGG 1 cut(s) 882
HphI GGTGA 2 cut(s) 37, 320
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 5 cut(s) 236, 820, 843, 1038, 1045
Hpy188III TCNNGA 5 cut(s) 35, 785, 875, 986, 1130
Hpy8I GTNNAC 1 cut(s) 520
HpyAV CCTTC 5 cut(s) 162, 299, 764, 1040, 1146
HpyCH4III ACNGT 7 cut(s) 406, 490, 503, 571, 670, 1162, 1182
HpyCH4IV ACGT 2 cut(s) 730, 851
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 2 cut(s) 730, 851
Hsp92II CATG 8 cut(s) 38, 71, 140, 176, 256, 478, 609, 995
Kzo9I GATC 3 cut(s) 72, 724, 903
LguI GCTCTTC 2 cut(s) 637, 1201
LweI GCATC 1 cut(s) 19
MaeI CTAG 1 cut(s) 713
MaeII ACGT 2 cut(s) 730, 851
MaeIII GTNAC 4 cut(s) 344, 545, 924, 1130
MalI GATC 3 cut(s) 74, 726, 905
MboI GATC 3 cut(s) 72, 724, 903
MboII GAAGA 9 cut(s) 136, 506, 582, 624, 807, 943, 1045, 1075, 1188
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 276
MlsI TGGCCA 1 cut(s) 1189
MluCI AATT 5 cut(s) 190, 279, 761, 940, 1039
MluNI TGGCCA 1 cut(s) 1189
MlyI GAGTC 1 cut(s) 948
Mox20I TGGCCA 1 cut(s) 1189
MroXI GAANNNNTTC 2 cut(s) 614, 1000
MscI TGGCCA 1 cut(s) 1189
MseI TTAA 5 cut(s) 110, 507, 675, 759, 1107
MslI CAYNNNNRTG 1 cut(s) 1163
Msp20I TGGCCA 1 cut(s) 1189
MspA1I CMGCKG 1 cut(s) 600
MspI CCGG 1 cut(s) 882
MspR9I CCNGG 2 cut(s) 704, 1210
Mva1269I GAATGC 4 cut(s) 479, 811, 1000, 1025
MvaI CCWGG 2 cut(s) 704, 1210
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 991
NdeII GATC 3 cut(s) 72, 724, 903
NlaIII CATG 8 cut(s) 38, 71, 140, 176, 256, 478, 609, 995
NlaIV GGNNCC 3 cut(s) 263, 413, 1167
NmuCI GTSAC 2 cut(s) 924, 1130
NspI RCATGY 1 cut(s) 256
NspV TTCGAA 1 cut(s) 1004
OliI CACNNNNGTG 1 cut(s) 1163
PaeI GCATGC 1 cut(s) 256
PaeR7I CTCGAG 1 cut(s) 875
PagI TCATGA 1 cut(s) 34
PasI CCCWGGG 1 cut(s) 703
PciSI GCTCTTC 2 cut(s) 637, 1201
PctI GAATGC 4 cut(s) 479, 811, 1000, 1025
PdmI GAANNNNTTC 2 cut(s) 614, 1000
PfeI GAWTC 4 cut(s) 560, 982, 1114, 1141
PflFI GACNNNGTC 1 cut(s) 515
PflMI CCANNNNNTGG 2 cut(s) 734, 992
PkrI GCNGC 3 cut(s) 258, 261, 386
PleI GAGTC 1 cut(s) 948
PpsI GAGTC 1 cut(s) 948
Psp6I CCWGG 2 cut(s) 702, 1208
PspGI CCWGG 2 cut(s) 702, 1208
PspN4I GGNNCC 3 cut(s) 263, 413, 1167
PspPI GGNCC 1 cut(s) 261
PstI CTGCAG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 72
PsyI GACNNNGTC 1 cut(s) 515
RsaI GTAC 3 cut(s) 604, 850, 950
RsaNI GTAC 3 cut(s) 603, 849, 949
RseI CAYNNNNRTG 1 cut(s) 1163
SapI GCTCTTC 2 cut(s) 637, 1201
SaqAI TTAA 5 cut(s) 110, 507, 675, 759, 1107
SatI GCNGC 3 cut(s) 257, 260, 385
Sau3AI GATC 3 cut(s) 72, 724, 903
Sau96I GGNCC 1 cut(s) 261
SchI GAGTC 1 cut(s) 948
ScrFI CCNGG 2 cut(s) 704, 1210
SduI GDGCHC 1 cut(s) 276
SfaNI GCATC 1 cut(s) 19
SfcI CTRYAG 3 cut(s) 550, 669, 1174
Sfr274I CTCGAG 1 cut(s) 875
SfuI TTCGAA 1 cut(s) 1004
SlaI CTCGAG 1 cut(s) 875
SmiMI CAYNNNNRTG 1 cut(s) 1163
SmlI CTYRAG 2 cut(s) 783, 875
SmoI CTYRAG 2 cut(s) 783, 875
SphI GCATGC 1 cut(s) 256
Sse9I AATT 5 cut(s) 190, 279, 761, 940, 1039
SsiI CCGC 5 cut(s) 256, 259, 276, 385, 600
SspMI CTAG 1 cut(s) 713
StyD4I CCNGG 2 cut(s) 702, 1208
StyI CCWWGG 2 cut(s) 653, 991
TaaI ACNGT 7 cut(s) 406, 490, 503, 571, 670, 1162, 1182
TaiI ACGT 2 cut(s) 733, 854
TaqI TCGA 9 cut(s) 122, 160, 178, 492, 876, 902, 1004, 1117, 1139
TasI AATT 5 cut(s) 190, 279, 761, 940, 1039
TauI GCSGC 3 cut(s) 259, 262, 387
TfiI GAWTC 4 cut(s) 560, 982, 1114, 1141
Tru1I TTAA 5 cut(s) 110, 507, 675, 759, 1107
Tru9I TTAA 5 cut(s) 110, 507, 675, 759, 1107
TscAI CASTG 3 cut(s) 493, 695, 1167
TseFI GTSAC 2 cut(s) 924, 1130
Tsp45I GTSAC 2 cut(s) 924, 1130
TspDTI ATGAA 5 cut(s) 51, 125, 272, 1022, 1045
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 3 cut(s) 493, 695, 1167
Tth111I GACNNNGTC 1 cut(s) 515
Van91I CCANNNNNTGG 2 cut(s) 734, 992
XapI RAATTY 2 cut(s) 940, 1039
XceI RCATGY 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 875
XmnI GAANNNNTTC 2 cut(s) 614, 1000
XspI CTAG 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.