RLG00000025748

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007822
Physical Location & Seq
Reverse (-)
48049589 .. 48050878
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025748

Sequence Viewer

Length: 1290 bp
ATGTTGGAAAGTGATGTTCATGCTGCGTTGACCTGCGATGACATCCTAAATGAGATACTTCTTCGGCTGCCAGAGAAATTTGTATTCAGATTGATACTTGTGTCAAAGAGGTGGCTTCGTGTGATATGTAGTTCTTCTTTTCGATCTGATTATCATGTTAGATGGAGAGTGAACTTTCATCTTCTGGGTTTCTTTGTCTGCAACCAGCTGTACCTTTGCAGACCTAAAGATGGGATGCACCGACCCAAATCTGAGCCTGCACTTCAGTTATTGACAGCTTGTGAGGAAGGTGATGATGTATGCAATGATTTGATGTCTTCTGGGAACTGCAAACAGCTTGGTTATTTCATCGACTCTGCCAATGGGCTTCTTCTTTGTGGCCGCCACCCATTGACTTATTCTGTCTGGAACCCAATCAGCAAAGTGCTATTCCAACTTCCTGAACCTCAGCGTTTCTACAAACGCTTGTGTATGGCATTCATTGCTGAGGACTATTTCAATGACATTCTTCACTACAAGGTTATTCGTGCGAATTGTGAATGCAAACGTGAGGTTAACACTGTCTCCATCGAGACTTTCTCTTCAGTGACTGGTACATGGAAGCATTTCACGCTCACTTGCTCTTCAAATTTTTCCTTGCGCCCCTGGACAGTGGCTACTATGATCAATGGTGTAGTATACTGGTATGCGACACAGGGAAAGATAGCCATTAAGGAAAGTTTAGCCATTTATGACCCACGTGTTGGAAACACGCGTATTGTTTTGCTTAAATTACCTGATGGGAAGATTTCACAGGATTATGATGAGTTTGTTCTTGGGGAGTCCTCAGATGGGCTATTGCAGTATGGTCAGAGCAGCAAGTCAGGCATGGAGATATGGGTTCTTGAGAAGGAACAAGATAGTATCTCATCTATTACCACAAGCAATGCGCAATCAAATCGCAAGTGGAAGTTTAGATACAGACTAAGTTTCAAATCAATGTGGAAGAAAAATCCAAGCTTTTGCAGGCAGACTAAAGAAGTGCAAATATTGTCATTCCTTCCTCAGAATTCTGAATATGTCTTCATAAGATCTGGACAAAACATACTTCTCTTGCACATTGAAAGCCAAATGTTGAAGGTGATTCGATATCACGGTCCTAGCTCTCTTATCCAATGGGATTTCAGCAAAGTGGTGCCTTACTTTCGACCTTCTTGGCCACGATCTTCTTTATGCCATGAAAAGGCGGCAACTTCTTCTTGGCCTTCTTCAGGCCATGAAACTGCGGCACCAGCTTGGCCTTCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

430

Amino Acids

49.53

Weight (kDa)

8.89

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 108 - 328 3.1e-10 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 110 - 299 4.3e-13 F-box protein At3g26010-like, beta-propeller
Beta-prop_AT5G49610-like PF23635 118 - 375 9e-07 F-box protein AT5G49610-like, beta-propeller
FBA_1 PF07734 120 - 361 1.6e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 930
Acc36I ACCTGC 1 cut(s) 41
AccB1I GGYRCC 2 cut(s) 1174, 1267
AccB7I CCANNNNNTGG 1 cut(s) 743
AccI GTMKAC 1 cut(s) 678
AccII CGCG 1 cut(s) 754
AciI CCGC 3 cut(s) 382, 1226, 1265
AcoI YGGCCR 2 cut(s) 379, 1196
AcsI RAATTY 3 cut(s) 77, 628, 1048
AcuI CTGAAG 3 cut(s) 248, 567, 1233
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 2 cut(s) 212, 595
AfiI CCNNNNNNNGG 5 cut(s) 230, 743, 831, 1222, 1250
AflIII ACRYGT 2 cut(s) 739, 752
AgsI TTSAA 5 cut(s) 499, 627, 973, 1103, 1117
AjnI CCWGG 2 cut(s) 644, 1283
AluBI AGCT 6 cut(s) 208, 278, 337, 999, 1143, 1274
AluI AGCT 6 cut(s) 208, 278, 337, 999, 1143, 1274
Alw26I GTCTC 2 cut(s) 566, 568
AlwNI CAGNNNCTG 1 cut(s) 590
AoxI GGCC 5 cut(s) 379, 1196, 1241, 1252, 1277
ApeKI GCWGC 3 cut(s) 23, 67, 855
ApoI RAATTY 3 cut(s) 77, 628, 1048
Asp700I GAANNNNTTC 1 cut(s) 605
AspLEI GCGC 2 cut(s) 642, 931
AspS9I GGNCC 1 cut(s) 1136
AsuHPI GGTGA 2 cut(s) 302, 1132
AvaII GGWCC 1 cut(s) 1136
BaeI ACNNNNGTAYC 2 cut(s) 86, 119
BalI TGGCCA 1 cut(s) 1198
BanI GGYRCC 2 cut(s) 1174, 1267
BarI GAAGNNNNNNTAC 2 cut(s) 941, 973
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 2 cut(s) 309, 1054
BbvCI CCTCAGC 2 cut(s) 447, 486
BbvI GCAGC 3 cut(s) 10, 54, 867
BccI CCATC 5 cut(s) 156, 224, 575, 773, 824
BcgI CGANNNNNNTGC 2 cut(s) 920, 954
BciT130I CCWGG 2 cut(s) 646, 1285
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 2 cut(s) 566, 568
BfaI CTAG 1 cut(s) 1140
BfuAI ACCTGC 1 cut(s) 41
BglII AGATCT 1 cut(s) 1070
BisI GCNGC 6 cut(s) 24, 68, 382, 856, 1227, 1266
BlsI GCNGC 6 cut(s) 25, 69, 383, 857, 1228, 1267
Bme1390I CCNGG 2 cut(s) 646, 1285
Bme18I GGWCC 1 cut(s) 1136
BmgT120I GGNCC 1 cut(s) 1136
BmiI GGNNCC 3 cut(s) 410, 1176, 1269
BmrFI CCNGG 2 cut(s) 646, 1285
BmsI GCATC 1 cut(s) 225
BpiI GAAGAC 2 cut(s) 309, 1054
BplI GAGNNNNNCTC 2 cut(s) 563, 595
Bpu10I CCTNAGC 2 cut(s) 447, 486
BpuEI CTTGAG 1 cut(s) 905
BsaAI YACGTR 1 cut(s) 740
BsaJI CCNNGG 1 cut(s) 644
BsaXI ACNNNNNCTCC 2 cut(s) 548, 578
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 743, 831, 1222, 1250
Bse1I ACTGG 2 cut(s) 595, 686
Bse3DI GCAATG 3 cut(s) 310, 480, 931
BseBI CCWGG 2 cut(s) 646, 1285
BseDI CCNNGG 1 cut(s) 644
BseGI GGATG 2 cut(s) 42, 240
BseLI CCNNNNNNNGG 5 cut(s) 230, 743, 831, 1222, 1250
BseMI GCAATG 3 cut(s) 310, 480, 931
BseMII CTCAG 5 cut(s) 243, 461, 477, 840, 1058
BseNI ACTGG 2 cut(s) 595, 686
BseXI GCAGC 3 cut(s) 10, 54, 867
BsgI GTGCAG 1 cut(s) 243
Bsh1236I CGCG 1 cut(s) 754
BshFI GGCC 5 cut(s) 381, 1198, 1243, 1254, 1279
BshNI GGYRCC 2 cut(s) 1174, 1267
BslI CCNNNNNNNGG 5 cut(s) 230, 743, 831, 1222, 1250
BsmAI GTCTC 2 cut(s) 566, 568
BsmI GAATGC 2 cut(s) 476, 545
BsnI GGCC 5 cut(s) 381, 1198, 1243, 1254, 1279
Bsp143I GATC 4 cut(s) 143, 663, 1070, 1202
BspACI CCGC 3 cut(s) 382, 1226, 1265
BspANI GGCC 5 cut(s) 381, 1198, 1243, 1254, 1279
BspCNI CTCAG 5 cut(s) 244, 460, 478, 839, 1057
BspFNI CGCG 1 cut(s) 754
BspLI GGNNCC 3 cut(s) 410, 1176, 1269
BspMI ACCTGC 1 cut(s) 41
BspQI GCTCTTC 1 cut(s) 628
BspT107I GGYRCC 2 cut(s) 1174, 1267
BsrDI GCAATG 3 cut(s) 310, 480, 931
BsrI ACTGG 2 cut(s) 595, 686
BssECI CCNNGG 1 cut(s) 644
BssMI GATC 4 cut(s) 143, 663, 1070, 1202
BssNAI GTATAC 1 cut(s) 679
Bst1107I GTATAC 1 cut(s) 679
Bst2UI CCWGG 2 cut(s) 646, 1285
Bst4CI ACNGT 3 cut(s) 562, 652, 1136
Bst6I CTCTTC 2 cut(s) 586, 628
BstAPI GCANNNNNTGC 1 cut(s) 482
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 2 cut(s) 258, 1007
BstDEI CTNAG 6 cut(s) 252, 447, 486, 826, 965, 1044
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 2 cut(s) 42, 240
BstFNI CGCG 1 cut(s) 754
BstHHI GCGC 2 cut(s) 642, 931
BstKTI GATC 4 cut(s) 146, 666, 1073, 1205
BstMAI GTCTC 2 cut(s) 566, 568
BstMBI GATC 4 cut(s) 143, 663, 1070, 1202
BstMWI GCNNNNNNNGC 4 cut(s) 482, 610, 864, 1271
BstNI CCWGG 2 cut(s) 646, 1285
BstSCI CCNGG 2 cut(s) 644, 1283
BstUI CGCG 1 cut(s) 754
BstV1I GCAGC 3 cut(s) 10, 54, 867
BstV2I GAAGAC 2 cut(s) 309, 1054
BstX2I RGATCY 1 cut(s) 1070
BstYI RGATCY 1 cut(s) 1070
BstZ17I GTATAC 1 cut(s) 679
BsuRI GGCC 5 cut(s) 381, 1198, 1243, 1254, 1279
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 42, 240
BtsIMutI CAGTG 3 cut(s) 558, 591, 657
BveI ACCTGC 1 cut(s) 41
Cac8I GCNNGC 2 cut(s) 258, 1007
CaiI CAGNNNCTG 1 cut(s) 590
CfoI GCGC 2 cut(s) 642, 931
Cfr13I GGNCC 1 cut(s) 1136
Csp6I GTAC 2 cut(s) 211, 594
CviAII CATG 6 cut(s) 20, 155, 597, 868, 1217, 1256
CviQI GTAC 2 cut(s) 211, 594
DdeI CTNAG 6 cut(s) 252, 447, 486, 826, 965, 1044
DpnI GATC 4 cut(s) 145, 665, 1072, 1204
DpnII GATC 4 cut(s) 143, 663, 1070, 1202
EaeI YGGCCR 2 cut(s) 379, 1196
Eam1104I CTCTTC 2 cut(s) 586, 628
EarI CTCTTC 2 cut(s) 586, 628
Eco32I GATATC 1 cut(s) 1130
Eco47I GGWCC 1 cut(s) 1136
Eco57I CTGAAG 3 cut(s) 248, 567, 1233
Eco72I CACGTG 1 cut(s) 740
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoRI GAATTC 1 cut(s) 1048
EcoRII CCWGG 2 cut(s) 644, 1283
EcoRV GATATC 1 cut(s) 1130
FaeI CATG 6 cut(s) 23, 158, 600, 871, 1220, 1259
FatI CATG 6 cut(s) 19, 154, 596, 867, 1216, 1255
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 6 cut(s) 24, 68, 382, 856, 1227, 1266
FokI GGATG 2 cut(s) 29, 247
Fsp4HI GCNGC 6 cut(s) 24, 68, 382, 856, 1227, 1266
FspBI CTAG 1 cut(s) 1140
FspI TGCGCA 1 cut(s) 930
GlaI GCGC 2 cut(s) 641, 930
GluI GCNGC 6 cut(s) 24, 68, 382, 856, 1227, 1266
HaeIII GGCC 5 cut(s) 381, 1198, 1243, 1254, 1279
HhaI GCGC 2 cut(s) 642, 931
Hin1II CATG 6 cut(s) 23, 158, 600, 871, 1220, 1259
Hin6I GCGC 2 cut(s) 640, 929
HinP1I GCGC 2 cut(s) 640, 929
HincII GTYRAC 2 cut(s) 30, 556
HindII GTYRAC 2 cut(s) 30, 556
HindIII AAGCTT 1 cut(s) 997
HinfI GANTC 3 cut(s) 353, 821, 1123
HpaI GTTAAC 1 cut(s) 556
HphI GGTGA 2 cut(s) 302, 1132
Hpy166II GTNNAC 4 cut(s) 30, 172, 556, 679
Hpy188I TCNGA 7 cut(s) 89, 148, 253, 829, 852, 1047, 1054
Hpy188III TCNNGA 5 cut(s) 406, 440, 571, 884, 1074
Hpy8I GTNNAC 4 cut(s) 30, 172, 556, 679
HpyAV CCTTC 7 cut(s) 281, 883, 1049, 1111, 1200, 1254, 1290
HpyCH4III ACNGT 3 cut(s) 562, 652, 1136
HpyCH4IV ACGT 2 cut(s) 547, 739
HpyF10VI GCNNNNNNNGC 4 cut(s) 482, 610, 864, 1271
HpyF3I CTNAG 6 cut(s) 252, 447, 486, 826, 965, 1044
HpySE526I ACGT 2 cut(s) 547, 739
Hsp92II CATG 6 cut(s) 23, 158, 600, 871, 1220, 1259
HspAI GCGC 2 cut(s) 640, 929
Ksp22I TGATCA 1 cut(s) 663
KspAI GTTAAC 1 cut(s) 556
Kzo9I GATC 4 cut(s) 143, 663, 1070, 1202
LguI GCTCTTC 1 cut(s) 628
Lsp1109I GCAGC 3 cut(s) 10, 54, 867
LweI GCATC 1 cut(s) 225
MaeI CTAG 1 cut(s) 1140
MaeII ACGT 2 cut(s) 547, 739
MaeIII GTNAC 1 cut(s) 586
MalI GATC 4 cut(s) 145, 665, 1072, 1204
MboI GATC 4 cut(s) 143, 663, 1070, 1202
MflI RGATCY 1 cut(s) 1070
MlsI TGGCCA 1 cut(s) 1198
MluCI AATT 5 cut(s) 77, 532, 628, 770, 1048
MluI ACGCGT 1 cut(s) 752
MluNI TGGCCA 1 cut(s) 1198
MlyI GAGTC 2 cut(s) 347, 830
MmeI TCCRAC 2 cut(s) 457, 724
MnlI CCTC 7 cut(s) 102, 277, 456, 481, 544, 835, 1053
Mox20I TGGCCA 1 cut(s) 1198
MroXI GAANNNNTTC 1 cut(s) 605
MscI TGGCCA 1 cut(s) 1198
MseI TTAA 3 cut(s) 555, 711, 768
Msp20I TGGCCA 1 cut(s) 1198
MspA1I CMGCKG 1 cut(s) 208
MspR9I CCNGG 2 cut(s) 646, 1285
Mva1269I GAATGC 2 cut(s) 476, 545
MvaI CCWGG 2 cut(s) 646, 1285
MvnI CGCG 1 cut(s) 754
MwoI GCNNNNNNNGC 4 cut(s) 482, 610, 864, 1271
NdeII GATC 4 cut(s) 143, 663, 1070, 1202
NlaIII CATG 6 cut(s) 23, 158, 600, 871, 1220, 1259
NlaIV GGNNCC 3 cut(s) 410, 1176, 1269
NmuCI GTSAC 1 cut(s) 586
NsbI TGCGCA 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 628
PctI GAATGC 2 cut(s) 476, 545
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 1 cut(s) 1123
PflMI CCANNNNNTGG 1 cut(s) 743
PkrI GCNGC 6 cut(s) 25, 69, 383, 857, 1228, 1267
PleI GAGTC 2 cut(s) 347, 829
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 2 cut(s) 347, 829
Ppu21I YACGTR 1 cut(s) 740
Psp6I CCWGG 2 cut(s) 644, 1283
PspCI CACGTG 1 cut(s) 740
PspGI CCWGG 2 cut(s) 644, 1283
PspN4I GGNNCC 3 cut(s) 410, 1176, 1269
PspPI GGNCC 1 cut(s) 1136
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 1 cut(s) 1070
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 2 cut(s) 212, 595
RsaNI GTAC 2 cut(s) 211, 594
SapI GCTCTTC 1 cut(s) 628
SaqAI TTAA 3 cut(s) 555, 711, 768
SatI GCNGC 6 cut(s) 24, 68, 382, 856, 1227, 1266
Sau3AI GATC 4 cut(s) 143, 663, 1070, 1202
Sau96I GGNCC 1 cut(s) 1136
SchI GAGTC 2 cut(s) 347, 830
ScrFI CCNGG 2 cut(s) 646, 1285
SfaNI GCATC 1 cut(s) 225
SinI GGWCC 1 cut(s) 1136
SmlI CTYRAG 1 cut(s) 884
SmoI CTYRAG 1 cut(s) 884
Sse9I AATT 5 cut(s) 77, 532, 628, 770, 1048
SsiI CCGC 3 cut(s) 382, 1226, 1265
SspI AATATT 1 cut(s) 1029
SspMI CTAG 1 cut(s) 1140
StyD4I CCNGG 2 cut(s) 644, 1283
TaaI ACNGT 3 cut(s) 562, 652, 1136
TaiI ACGT 2 cut(s) 550, 742
TaqI TCGA 5 cut(s) 142, 351, 570, 1126, 1186
TasI AATT 5 cut(s) 77, 532, 628, 770, 1048
TauI GCSGC 3 cut(s) 384, 1229, 1268
TfiI GAWTC 1 cut(s) 1123
Tru1I TTAA 3 cut(s) 555, 711, 768
Tru9I TTAA 3 cut(s) 555, 711, 768
TscAI CASTG 3 cut(s) 565, 591, 657
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 3 cut(s) 23, 67, 855
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 7 cut(s) 8, 167, 337, 469, 1054, 1233, 1272
TspRI CASTG 3 cut(s) 565, 591, 657
Van91I CCANNNNNTGG 1 cut(s) 743
VpaK11BI GGWCC 1 cut(s) 1136
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 3 cut(s) 77, 628, 1048
XmiI GTMKAC 1 cut(s) 678
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.