Rh3CG025200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0007822
Physical Location & Seq
Forward (+)
1723869 .. 1726467
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG025200.1

Sequence Viewer

Length: 1320 bp
ATGAGTCGACAAGAGAAAAAGAAGGAAAAGATGTTGGAAAGTGATGTTCATGCTGCATTGACCTGCGATGACATCATAAATGAGATACTTCTTCGCCTGCCAGAGAAATTTGTATTCAGATTGATACTTGTGTCAAAGAGGTGGCTTCGTGTGATATGTAGTTCTTCTTTTCGATCTGATTATCATGCTAGATGTAGAGTGAACTTTCATCTTCTGGGTTTCTTTGTCTGCAACCAGCTGTACCTTTGCAGACCTAAAGATGGGATGCACCGACCCAAATCTGAGCCTGCACTTCAGTTATTGACAGCTTGTGAGGAAGGTGATGATGTATGCAATGATTTGATGTCTTCTGGGAACTGCAAACAGCTTGGTTATTTCATCGACTCTGCCAATGGGCTTCTTCTTTGTGGCCGCCACCCATTGACTTATTCTGTCTGGAACCCAATCAGCAAAGTGCTATTCCAACTTCCTCAACCTCAGCGTTTCTACAAACGCTTGTGTATGGCATTCATCGCTGAGGACTATTTCAATGACATTCTTCACTACAAGGTTATTCGTGCAAATTGTGAATGCAAACGTGAGGTTAACACTGTCTCCATCGAGACTTTCTCTTCAGTGACTGGTACATGGAAGCATTTCACGCTCACTTGCTCTTCAAAATTTTCCTTGCGCCCCTGGACAGTGGCTACTGTGATCAATGGTGTAGTATACTGGTATGCGACACAGGGAAAGATAGCCATTAAGGAAAGTTTAGCCATTTATGACCCACGTGTTGGAAACACGCGTATAGTTTTGCTTAAATTACCTGATGGGAAGATTTCGCAGGATTATGATGAGTTTGTTCTTGGGGAGTCCTCAGATGGGCTATTGCAGTATGGTCAGAGCAGCAAGTCAGGCATGGAGATATGGGTTCTTGAGAAGGAACAAGATAGTATCTCATCTATTACCACAAGCAATGCGCAATCAAATCGCAAGTGGAAGTTTAGATACAGACTAAGTTTCAAATCAATGTGGAAGAAAAATCCAAGCTTTTGCAGGCAGACTAAAGAAGCGCAAATATTGTCATTCCTTCCTCAGAATTCTGAATATGTCTTCATAAGATCTGGACAAAACATACTTCTCTTGCACATTGAAAGCCAAATGTTGAAGGTGATTCGATATCACGGTCCTAGCTCTCTTATCCAATGGGATTTCAGCAAAGTGGTGCCTTACTTTCGACCTTCTTGGCCACGATCTTCTTTATGCCATGAAAAGGCGGCAACTTCTTCTTGGCCTTCTTCAGGCCATGAAACTGCGGCACCTGCTTGGCCTTCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

439

Amino Acids

50.65

Weight (kDa)

9.12

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 118 - 337 3.2e-10 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 120 - 309 3e-13 F-box protein At3g26010-like, beta-propeller
Beta-prop_AT5G49610-like PF23635 128 - 385 6.6e-07 F-box protein AT5G49610-like, beta-propeller
FBA_1 PF07734 130 - 371 1.4e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1309
Acc16I TGCGCA 1 cut(s) 960
Acc36I ACCTGC 2 cut(s) 71, 1309
AccB1I GGYRCC 2 cut(s) 1204, 1297
AccB7I CCANNNNNTGG 1 cut(s) 773
AccI GTMKAC 2 cut(s) 7, 708
AccII CGCG 1 cut(s) 784
AciI CCGC 3 cut(s) 412, 1256, 1295
AcoI YGGCCR 2 cut(s) 409, 1226
AcsI RAATTY 3 cut(s) 107, 659, 1078
AcuI CTGAAG 3 cut(s) 278, 597, 1263
AcvI CACGTG 1 cut(s) 770
AfaI GTAC 2 cut(s) 242, 625
AfiI CCNNNNNNNGG 5 cut(s) 260, 773, 861, 1252, 1280
AflIII ACRYGT 2 cut(s) 769, 782
AgsI TTSAA 5 cut(s) 529, 657, 1003, 1133, 1147
AjnI CCWGG 2 cut(s) 674, 1313
AluBI AGCT 5 cut(s) 238, 308, 367, 1029, 1173
AluI AGCT 5 cut(s) 238, 308, 367, 1029, 1173
Alw26I GTCTC 2 cut(s) 596, 598
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 5 cut(s) 409, 1226, 1271, 1282, 1307
ApeKI GCWGC 2 cut(s) 53, 885
ApoI RAATTY 3 cut(s) 107, 659, 1078
Asp700I GAANNNNTTC 1 cut(s) 635
AspLEI GCGC 3 cut(s) 672, 961, 1054
AspS9I GGNCC 1 cut(s) 1166
AsuHPI GGTGA 2 cut(s) 332, 1162
AvaII GGWCC 1 cut(s) 1166
BaeI ACNNNNGTAYC 2 cut(s) 116, 149
BalI TGGCCA 1 cut(s) 1228
BanI GGYRCC 2 cut(s) 1204, 1297
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BbrPI CACGTG 1 cut(s) 770
BbsI GAAGAC 2 cut(s) 339, 1084
BbvCI CCTCAGC 2 cut(s) 477, 516
BbvI GCAGC 2 cut(s) 40, 897
BccI CCATC 4 cut(s) 254, 605, 803, 854
BcgI CGANNNNNNTGC 2 cut(s) 950, 984
BciT130I CCWGG 2 cut(s) 676, 1315
BclI TGATCA 1 cut(s) 693
BcoDI GTCTC 2 cut(s) 596, 598
BfaI CTAG 2 cut(s) 189, 1170
BfuAI ACCTGC 2 cut(s) 71, 1309
BglII AGATCT 1 cut(s) 1100
BisI GCNGC 5 cut(s) 54, 412, 886, 1257, 1296
BlsI GCNGC 5 cut(s) 55, 413, 887, 1258, 1297
Bme1390I CCNGG 2 cut(s) 676, 1315
Bme18I GGWCC 1 cut(s) 1166
BmgT120I GGNCC 1 cut(s) 1166
BmiI GGNNCC 3 cut(s) 440, 1206, 1299
BmrFI CCNGG 2 cut(s) 676, 1315
BmsI GCATC 1 cut(s) 255
BpiI GAAGAC 2 cut(s) 339, 1084
BplI GAGNNNNNCTC 2 cut(s) 593, 625
Bpu10I CCTNAGC 2 cut(s) 477, 516
BpuEI CTTGAG 1 cut(s) 935
BsaAI YACGTR 1 cut(s) 770
BsaJI CCNNGG 1 cut(s) 674
BsaXI ACNNNNNCTCC 2 cut(s) 578, 608
Bsc4I CCNNNNNNNGG 5 cut(s) 260, 773, 861, 1252, 1280
Bse1I ACTGG 2 cut(s) 625, 716
Bse3DI GCAATG 2 cut(s) 340, 961
BseBI CCWGG 2 cut(s) 676, 1315
BseDI CCNNGG 1 cut(s) 674
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 5 cut(s) 260, 773, 861, 1252, 1280
BseMI GCAATG 2 cut(s) 340, 961
BseMII CTCAG 5 cut(s) 273, 491, 507, 870, 1088
BseNI ACTGG 2 cut(s) 625, 716
BseXI GCAGC 2 cut(s) 40, 897
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 784
BshFI GGCC 5 cut(s) 411, 1228, 1273, 1284, 1309
BshNI GGYRCC 2 cut(s) 1204, 1297
BslI CCNNNNNNNGG 5 cut(s) 260, 773, 861, 1252, 1280
BsmAI GTCTC 2 cut(s) 596, 598
BsmI GAATGC 2 cut(s) 506, 575
BsnI GGCC 5 cut(s) 411, 1228, 1273, 1284, 1309
Bsp143I GATC 4 cut(s) 173, 693, 1100, 1232
BspACI CCGC 3 cut(s) 412, 1256, 1295
BspANI GGCC 5 cut(s) 411, 1228, 1273, 1284, 1309
BspCNI CTCAG 5 cut(s) 274, 490, 508, 869, 1087
BspFNI CGCG 1 cut(s) 784
BspLI GGNNCC 3 cut(s) 440, 1206, 1299
BspMI ACCTGC 2 cut(s) 71, 1309
BspQI GCTCTTC 1 cut(s) 658
BspT107I GGYRCC 2 cut(s) 1204, 1297
BsrDI GCAATG 2 cut(s) 340, 961
BsrI ACTGG 2 cut(s) 625, 716
BssECI CCNNGG 1 cut(s) 674
BssMI GATC 4 cut(s) 173, 693, 1100, 1232
BssNAI GTATAC 1 cut(s) 709
Bst1107I GTATAC 1 cut(s) 709
Bst2UI CCWGG 2 cut(s) 676, 1315
Bst4CI ACNGT 4 cut(s) 592, 682, 691, 1166
Bst6I CTCTTC 2 cut(s) 616, 658
BstBAI YACGTR 1 cut(s) 770
BstC8I GCNNGC 3 cut(s) 98, 288, 1037
BstDEI CTNAG 6 cut(s) 282, 477, 516, 856, 995, 1074
BstENI CCTNNNNNAGG 1 cut(s) 1278
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 784
BstHHI GCGC 3 cut(s) 672, 961, 1054
BstKTI GATC 4 cut(s) 176, 696, 1103, 1235
BstMAI GTCTC 2 cut(s) 596, 598
BstMBI GATC 4 cut(s) 173, 693, 1100, 1232
BstMWI GCNNNNNNNGC 4 cut(s) 512, 640, 894, 1301
BstNI CCWGG 2 cut(s) 676, 1315
BstSCI CCNGG 2 cut(s) 674, 1313
BstUI CGCG 1 cut(s) 784
BstV1I GCAGC 2 cut(s) 40, 897
BstV2I GAAGAC 2 cut(s) 339, 1084
BstX2I RGATCY 1 cut(s) 1100
BstYI RGATCY 1 cut(s) 1100
BstZ17I GTATAC 1 cut(s) 709
BsuRI GGCC 5 cut(s) 411, 1228, 1273, 1284, 1309
BtgZI GCGATG 2 cut(s) 81, 496
BtsCI GGATG 1 cut(s) 270
BtsIMutI CAGTG 3 cut(s) 588, 621, 687
BveI ACCTGC 2 cut(s) 71, 1309
Cac8I GCNNGC 3 cut(s) 98, 288, 1037
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 3 cut(s) 672, 961, 1054
Cfr13I GGNCC 1 cut(s) 1166
Csp6I GTAC 2 cut(s) 241, 624
CviAII CATG 6 cut(s) 50, 185, 627, 898, 1247, 1286
CviQI GTAC 2 cut(s) 241, 624
DdeI CTNAG 6 cut(s) 282, 477, 516, 856, 995, 1074
DpnI GATC 4 cut(s) 175, 695, 1102, 1234
DpnII GATC 4 cut(s) 173, 693, 1100, 1232
EaeI YGGCCR 2 cut(s) 409, 1226
Eam1104I CTCTTC 2 cut(s) 616, 658
EarI CTCTTC 2 cut(s) 616, 658
Eco32I GATATC 1 cut(s) 1160
Eco47I GGWCC 1 cut(s) 1166
Eco57I CTGAAG 3 cut(s) 278, 597, 1263
Eco72I CACGTG 1 cut(s) 770
EcoNI CCTNNNNNAGG 1 cut(s) 1278
EcoRI GAATTC 1 cut(s) 1078
EcoRII CCWGG 2 cut(s) 674, 1313
EcoRV GATATC 1 cut(s) 1160
FaeI CATG 6 cut(s) 53, 188, 630, 901, 1250, 1289
FatI CATG 6 cut(s) 49, 184, 626, 897, 1246, 1285
FbaI TGATCA 1 cut(s) 693
FblI GTMKAC 2 cut(s) 7, 708
Fnu4HI GCNGC 5 cut(s) 54, 412, 886, 1257, 1296
FokI GGATG 1 cut(s) 277
Fsp4HI GCNGC 5 cut(s) 54, 412, 886, 1257, 1296
FspBI CTAG 2 cut(s) 189, 1170
FspI TGCGCA 1 cut(s) 960
GlaI GCGC 3 cut(s) 671, 960, 1053
GluI GCNGC 5 cut(s) 54, 412, 886, 1257, 1296
HaeIII GGCC 5 cut(s) 411, 1228, 1273, 1284, 1309
HhaI GCGC 3 cut(s) 672, 961, 1054
Hin1II CATG 6 cut(s) 53, 188, 630, 901, 1250, 1289
Hin6I GCGC 3 cut(s) 670, 959, 1052
HinP1I GCGC 3 cut(s) 670, 959, 1052
HincII GTYRAC 2 cut(s) 8, 586
HindII GTYRAC 2 cut(s) 8, 586
HindIII AAGCTT 1 cut(s) 1027
HinfI GANTC 4 cut(s) 4, 383, 851, 1153
HpaI GTTAAC 1 cut(s) 586
HphI GGTGA 2 cut(s) 332, 1162
Hpy166II GTNNAC 4 cut(s) 8, 202, 586, 709
Hpy188I TCNGA 7 cut(s) 119, 178, 283, 859, 882, 1077, 1084
Hpy188III TCNNGA 4 cut(s) 436, 601, 914, 1104
Hpy8I GTNNAC 4 cut(s) 8, 202, 586, 709
HpyAV CCTTC 8 cut(s) 16, 311, 913, 1079, 1141, 1230, 1284, 1320
HpyCH4III ACNGT 4 cut(s) 592, 682, 691, 1166
HpyCH4IV ACGT 2 cut(s) 577, 769
HpyF10VI GCNNNNNNNGC 4 cut(s) 512, 640, 894, 1301
HpyF3I CTNAG 6 cut(s) 282, 477, 516, 856, 995, 1074
HpySE526I ACGT 2 cut(s) 577, 769
Hsp92II CATG 6 cut(s) 53, 188, 630, 901, 1250, 1289
HspAI GCGC 3 cut(s) 670, 959, 1052
Ksp22I TGATCA 1 cut(s) 693
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 4 cut(s) 173, 693, 1100, 1232
LguI GCTCTTC 1 cut(s) 658
Lsp1109I GCAGC 2 cut(s) 40, 897
LweI GCATC 1 cut(s) 255
MaeI CTAG 2 cut(s) 189, 1170
MaeII ACGT 2 cut(s) 577, 769
MaeIII GTNAC 1 cut(s) 616
MalI GATC 4 cut(s) 175, 695, 1102, 1234
MboI GATC 4 cut(s) 173, 693, 1100, 1232
MflI RGATCY 1 cut(s) 1100
MlsI TGGCCA 1 cut(s) 1228
MluCI AATT 5 cut(s) 107, 562, 659, 800, 1078
MluI ACGCGT 1 cut(s) 782
MluNI TGGCCA 1 cut(s) 1228
MlyI GAGTC 3 cut(s) 13, 377, 860
MmeI TCCRAC 3 cut(s) 15, 487, 754
MnlI CCTC 8 cut(s) 132, 307, 480, 486, 511, 574, 865, 1083
Mox20I TGGCCA 1 cut(s) 1228
MroXI GAANNNNTTC 1 cut(s) 635
MscI TGGCCA 1 cut(s) 1228
MseI TTAA 3 cut(s) 585, 741, 798
Msp20I TGGCCA 1 cut(s) 1228
MspA1I CMGCKG 1 cut(s) 238
MspR9I CCNGG 2 cut(s) 676, 1315
Mva1269I GAATGC 2 cut(s) 506, 575
MvaI CCWGG 2 cut(s) 676, 1315
MvnI CGCG 1 cut(s) 784
MwoI GCNNNNNNNGC 4 cut(s) 512, 640, 894, 1301
NdeII GATC 4 cut(s) 173, 693, 1100, 1232
NlaIII CATG 6 cut(s) 53, 188, 630, 901, 1250, 1289
NlaIV GGNNCC 3 cut(s) 440, 1206, 1299
NmuCI GTSAC 1 cut(s) 616
NsbI TGCGCA 1 cut(s) 960
PaqCI CACCTGC 1 cut(s) 1309
PciSI GCTCTTC 1 cut(s) 658
PctI GAATGC 2 cut(s) 506, 575
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 1 cut(s) 1153
PflMI CCANNNNNTGG 1 cut(s) 773
PkrI GCNGC 5 cut(s) 55, 413, 887, 1258, 1297
PleI GAGTC 3 cut(s) 12, 377, 859
PmaCI CACGTG 1 cut(s) 770
PmlI CACGTG 1 cut(s) 770
PpsI GAGTC 3 cut(s) 12, 377, 859
Ppu21I YACGTR 1 cut(s) 770
Psp6I CCWGG 2 cut(s) 674, 1313
PspCI CACGTG 1 cut(s) 770
PspGI CCWGG 2 cut(s) 674, 1313
PspN4I GGNNCC 3 cut(s) 440, 1206, 1299
PspPI GGNCC 1 cut(s) 1166
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 1100
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 2 cut(s) 242, 625
RsaNI GTAC 2 cut(s) 241, 624
SalI GTCGAC 1 cut(s) 6
SapI GCTCTTC 1 cut(s) 658
SaqAI TTAA 3 cut(s) 585, 741, 798
SatI GCNGC 5 cut(s) 54, 412, 886, 1257, 1296
Sau3AI GATC 4 cut(s) 173, 693, 1100, 1232
Sau96I GGNCC 1 cut(s) 1166
SchI GAGTC 3 cut(s) 13, 377, 860
ScrFI CCNGG 2 cut(s) 676, 1315
SfaNI GCATC 1 cut(s) 255
SinI GGWCC 1 cut(s) 1166
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
Sse9I AATT 5 cut(s) 107, 562, 659, 800, 1078
SsiI CCGC 3 cut(s) 412, 1256, 1295
SspI AATATT 1 cut(s) 1059
SspMI CTAG 2 cut(s) 189, 1170
StyD4I CCNGG 2 cut(s) 674, 1313
TaaI ACNGT 4 cut(s) 592, 682, 691, 1166
TaiI ACGT 2 cut(s) 580, 772
TaqI TCGA 6 cut(s) 7, 172, 381, 600, 1156, 1216
TasI AATT 5 cut(s) 107, 562, 659, 800, 1078
TauI GCSGC 3 cut(s) 414, 1259, 1298
TfiI GAWTC 1 cut(s) 1153
Tru1I TTAA 3 cut(s) 585, 741, 798
Tru9I TTAA 3 cut(s) 585, 741, 798
TscAI CASTG 3 cut(s) 595, 621, 687
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 2 cut(s) 53, 885
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 7 cut(s) 38, 197, 367, 499, 1084, 1263, 1302
TspRI CASTG 3 cut(s) 595, 621, 687
Van91I CCANNNNNTGG 1 cut(s) 773
VpaK11BI GGWCC 1 cut(s) 1166
XagI CCTNNNNNAGG 1 cut(s) 1278
XapI RAATTY 3 cut(s) 107, 659, 1078
XmiI GTMKAC 2 cut(s) 7, 708
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 2 cut(s) 189, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.