FvH4_6g04682

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017678
Physical Location & Seq
Forward (+)
2588805 .. 2592241
3437 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04682.t1

Sequence Viewer

Length: 1725 bp
ATGGAGAGGAACAAAGGGGAGGCAATCAGGGCAATGCAACTTTCGGAATTGAAAATTCAAGAGAATGATTTTTCTGGAGCAAGGGAAATGGCAAGGATAGCTCACCAGCTCTTCCCTGAGGATGAGAACATTTGTAAATTACTTGCACTCTCTGAAGTCTACTATTCAATTTTTGAGTCTGGTCAAAGCCGTGGGACTGGAGATACTGCTAGAGCTGATTTTGAACAATTAGTCGAAAAGGTCAGTAGTCCTTCTGGAACTCTAAATGATGACGACGACGATGACGAAAGTGATGATGGCGATGATGATAGTGATTATGATTATGATGATGATGACGATGAGGACGAGGACGAGGACGATGATTTTGATGGCAACAATGAGTATGATGATCATGATGATCATGAGGATGATGGTGATGATGATTTTGTGATTGCTCCCAAAAGATTGAGGGAGAGACAATTGAAGAGTTATACTGAGAAGAAAGAGAAGAACTCAGTGGAAGGTGGGGTATCTAAAAGTGCTGTGAGTCATCCAAAAAGGTTGAGGCAGAGCCAATTCCTCAGTGTTATTAACAGAATGAATGACACACTTAAGGATCATGATTCTATCAGTTCCATGAAAAGCTCTTCTACCCCTCCATCTTCTTCAGCTGCTCAATTCTATGACTTTAATGCACAGAAGTCTGAGGAGAAGATTAAGGCTGGTCAAATATGGGCAATGTATAGTGATGGGAATGGAATGCCTAAGACCTTTGCTCAAGTTAAGAAGGTTAATGTCAAGCCAAACAGAAGAGTTCAGAGGACTGTGTCTTCGGGGCAATTTTATTTGCGAATGGGATTGCTTGAATCTTTCTCGGAGCCCAGAAACATGGTTCAGCCGGTTTGTTGTGGGATATTTAAAGTCAAACGAGCTCCACCTACACTGTTCTCTGTCACCTCATTATCTCATCGTATCAAACCCAAACTTTTGGGCCACAACATATTTGAAATAATTCCTAGGAGAGGTGAAGTTTGGGCACTATACAAGGAGCACAATCCACTGGTGACCTGTCCTAACATGGGGCAGGGTGAATGTCTAGTGGTGGAAGTGCTAGAAGCTGTGCATCGGAGTACAAAGATTGTGGTTCTGGAACAGTTTAATGGATTTAAGTCAGTATTCAAGGCTCCAACAGTGCAGAGTACAAAAACTGGTGTCATTGATATACCGCAGGATGAGTTGGGTAGATTCTCTCACCAGATTTCTGCTTTTAAGCATGCCAAGATGAGTAACAGTCGACTTGCAGGGTGTTGGGAGCTTGATCCGTTATCAGTTTCTGGTTATGAGAAAAATGGTGTTCCCAAATGGCTTTTTGATTGTGATCCTGCCTCTGTGCTGAACACTCCTGACTACTCCCGTCACCTTGGTGATATGAAAATGGATCAAACAAGCATGAATTCTGAAACTCATGGTCTGTCTGGTGAATTCCTTGACGTTAAAGCTAAACCTGAAATGGGTTCTCCAAGCATACAAACAGGTAACAAACAGGAGACACCTTTTGCTGAAATAGAGACTTCTGAGAAAGTTGAATCACGGGTTTGTCACTTCTCCTTCTTTGGTAATGATTTGGCGGATTCTGTTAAGCTAGAAGCAAGTCAATATGTTAATGCTGTAGACAATGGCATTCATATTACAAAAGATGGCAACCTCACCCACCCAAAGGGAAGTGCTATTCCACTGTTAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

575

Amino Acids

64.22

Weight (kDa)

4.93

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 217 - 419 6e-42 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017678)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04682
rosa_chinensis RchiOBHm_Chr3g0453161
rosa_laevigata RLG00000025532
rosa_multiflora Rmu_co8446773.1_g000001 Rmu_sc0007132.1_g000008
rosa_roxburghii Rroxscaffold_6G00430220
rosa_rugosa Rorug02G0646800
rosa_samantha Rh3AG049600 Rh3BG050800 Rh3CG051000 Rh3DG051600
rosa_wichuraiana Rw3G003810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 159, 1273, 1650
AciI CCGC 2 cut(s) 1205, 1607
AclWI GGATC 4 cut(s) 603, 1292, 1352, 1425
AcsI RAATTY 3 cut(s) 54, 1432, 1460
AcuI CTGAAG 2 cut(s) 174, 630
AfaI GTAC 2 cut(s) 1111, 1180
AfiI CCNNNNNNNGG 5 cut(s) 1001, 1058, 1490, 1696, 1718
AflII CTTAAG 1 cut(s) 590
AgsI TTSAA 9 cut(s) 52, 59, 168, 224, 463, 845, 986, 1159, 1565
AleI CACNNNNGTG 1 cut(s) 1401
Alw21I GWGCWC 2 cut(s) 913, 1032
Alw26I GTCTC 3 cut(s) 448, 1520, 1541
AlwI GGATC 4 cut(s) 603, 1292, 1352, 1425
AlwNI CAGNNNCTG 1 cut(s) 1313
AoxI GGCC 1 cut(s) 970
ApeKI GCWGC 1 cut(s) 650
ApoI RAATTY 3 cut(s) 54, 1432, 1460
ArsI GACNNNNNNTTYG 4 cut(s) 347, 379, 793, 825
Asp700I GAANNNNTTC 1 cut(s) 990
AspA2I CCTAGG 1 cut(s) 995
AspS9I GGNCC 1 cut(s) 970
AvrII CCTAGG 1 cut(s) 995
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 1018
BanII GRGCYC 2 cut(s) 861, 913
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 2 cut(s) 913, 1032
BbvI GCAGC 1 cut(s) 637
BccI CCATC 6 cut(s) 290, 362, 404, 646, 722, 1670
BceAI ACGGC 1 cut(s) 174
BclI TGATCA 2 cut(s) 388, 397
BcoDI GTCTC 3 cut(s) 448, 1520, 1541
BfaI CTAG 5 cut(s) 210, 996, 1076, 1091, 1622
BfmI CTRYAG 1 cut(s) 1647
BfrI CTTAAG 1 cut(s) 590
BisI GCNGC 1 cut(s) 651
BlnI CCTAGG 1 cut(s) 995
BlsI GCNGC 1 cut(s) 652
BmgT120I GGNCC 1 cut(s) 970
BmiI GGNNCC 2 cut(s) 858, 1164
BmsI GCATC 1 cut(s) 1111
BpiI GAAGAC 1 cut(s) 801
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpmI CTGGAG 2 cut(s) 96, 219
BpuEI CTTGAG 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 1356
BsaJI CCNNGG 3 cut(s) 190, 995, 1399
Bsc4I CCNNNNNNNGG 5 cut(s) 1001, 1058, 1490, 1696, 1718
Bse118I RCCGGY 1 cut(s) 877
Bse1I ACTGG 3 cut(s) 202, 1044, 1192
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 2 cut(s) 39, 723
Bse8I GATNNNNATC 1 cut(s) 1356
BseDI CCNNGG 3 cut(s) 190, 995, 1399
BseGI GGATG 4 cut(s) 127, 412, 529, 1216
BseJI GATNNNNATC 1 cut(s) 1356
BseLI CCNNNNNNNGG 5 cut(s) 1001, 1058, 1490, 1696, 1718
BseMI GCAATG 2 cut(s) 39, 723
BseMII CTCAG 6 cut(s) 108, 465, 507, 574, 675, 1545
BseNI ACTGG 3 cut(s) 202, 1044, 1192
BseRI GAGGAG 1 cut(s) 701
BseSI GKGCMC 1 cut(s) 1018
BseXI GCAGC 1 cut(s) 637
BsgI GTGCAG 1 cut(s) 1193
BshFI GGCC 1 cut(s) 972
BsiHKAI GWGCWC 2 cut(s) 913, 1032
BsiSI CCGG 1 cut(s) 878
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 5 cut(s) 1001, 1058, 1490, 1696, 1718
BsmAI GTCTC 3 cut(s) 448, 1520, 1541
BsmFI GGGAC 1 cut(s) 208
BsmI GAATGC 2 cut(s) 744, 1659
BsnI GGCC 1 cut(s) 972
Bsp1286I GDGCHC 4 cut(s) 861, 913, 1018, 1032
Bsp143I GATC 6 cut(s) 388, 397, 595, 1297, 1357, 1417
BspACI CCGC 2 cut(s) 1205, 1607
BspANI GGCC 1 cut(s) 972
BspCNI CTCAG 6 cut(s) 109, 466, 506, 573, 676, 1546
BspHI TCATGA 3 cut(s) 391, 400, 598
BspLI GGNNCC 2 cut(s) 858, 1164
BspPI GGATC 4 cut(s) 603, 1292, 1352, 1425
BspQI GCTCTTC 2 cut(s) 116, 631
BspTI CTTAAG 1 cut(s) 590
BsrDI GCAATG 2 cut(s) 39, 723
BsrFI RCCGGY 1 cut(s) 877
BsrI ACTGG 3 cut(s) 202, 1044, 1192
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 3 cut(s) 190, 995, 1399
BssMI GATC 6 cut(s) 388, 397, 595, 1297, 1357, 1417
BssT1I CCWWGG 2 cut(s) 995, 1399
Bst4CI ACNGT 6 cut(s) 805, 924, 1134, 1171, 1271, 1716
Bst6I CTCTTC 4 cut(s) 116, 458, 631, 784
BstAFI CTTAAG 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 1254
BstDEI CTNAG 7 cut(s) 117, 474, 493, 560, 684, 744, 1554
BstDSI CCRYGG 1 cut(s) 190
BstEII GGTNACC 1 cut(s) 1042
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 4 cut(s) 127, 412, 529, 1216
BstKTI GATC 6 cut(s) 391, 400, 598, 1300, 1360, 1420
BstMAI GTCTC 3 cut(s) 448, 1520, 1541
BstMBI GATC 6 cut(s) 388, 397, 595, 1297, 1357, 1417
BstMWI GCNNNNNNNGC 2 cut(s) 29, 98
BstNSI RCATGY 1 cut(s) 1256
BstPI GGTNACC 1 cut(s) 1042
BstSFI CTRYAG 1 cut(s) 1647
BstSLI GKGCMC 1 cut(s) 1018
BstV1I GCAGC 1 cut(s) 637
BstV2I GAAGAC 1 cut(s) 801
BstXI CCANNNNNNTGG 2 cut(s) 868, 967
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 190
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 4 cut(s) 127, 412, 529, 1216
BtsIMutI CAGTG 6 cut(s) 501, 568, 920, 1037, 1176, 1712
Cac8I GCNNGC 1 cut(s) 1254
CaiI CAGNNNCTG 1 cut(s) 1313
CciI TCATGA 3 cut(s) 391, 400, 598
Cfr10I RCCGGY 1 cut(s) 877
Cfr13I GGNCC 1 cut(s) 970
Csp6I GTAC 2 cut(s) 1110, 1179
CspCI CAANNNNNGTGG 2 cut(s) 1101, 1136
CviAII CATG 9 cut(s) 392, 401, 599, 616, 868, 1057, 1253, 1429, 1445
CviQI GTAC 2 cut(s) 1110, 1179
DdeI CTNAG 7 cut(s) 117, 474, 493, 560, 684, 744, 1554
DpnI GATC 6 cut(s) 390, 399, 597, 1299, 1359, 1419
DpnII GATC 6 cut(s) 388, 397, 595, 1297, 1357, 1417
DraI TTTAAA 1 cut(s) 898
Eam1104I CTCTTC 4 cut(s) 116, 458, 631, 784
EarI CTCTTC 4 cut(s) 116, 458, 631, 784
EciI GGCGGA 1 cut(s) 1622
Ecl136II GAGCTC 1 cut(s) 911
Eco130I CCWWGG 2 cut(s) 995, 1399
Eco24I GRGCYC 2 cut(s) 861, 913
Eco53kI GAGCTC 1 cut(s) 911
Eco57I CTGAAG 2 cut(s) 174, 630
Eco81I CCTNAGG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 1042
EcoICRI GAGCTC 1 cut(s) 911
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoO65I GGTNACC 1 cut(s) 1042
EcoRI GAATTC 2 cut(s) 1432, 1460
EcoT14I CCWWGG 2 cut(s) 995, 1399
EcoT38I GRGCYC 2 cut(s) 861, 913
ErhI CCWWGG 2 cut(s) 995, 1399
FaeI CATG 9 cut(s) 395, 404, 602, 619, 871, 1060, 1256, 1432, 1448
FaqI GGGAC 1 cut(s) 208
FatI CATG 9 cut(s) 391, 400, 598, 615, 867, 1056, 1252, 1428, 1444
FbaI TGATCA 2 cut(s) 388, 397
FblI GTMKAC 3 cut(s) 159, 1273, 1650
Fnu4HI GCNGC 1 cut(s) 651
FokI GGATG 4 cut(s) 134, 419, 516, 1223
FriOI GRGCYC 2 cut(s) 861, 913
Fsp4HI GCNGC 1 cut(s) 651
FspBI CTAG 5 cut(s) 210, 996, 1076, 1091, 1622
GluI GCNGC 1 cut(s) 651
GsuI CTGGAG 2 cut(s) 96, 219
HaeIII GGCC 1 cut(s) 972
HapII CCGG 1 cut(s) 878
Hin1II CATG 9 cut(s) 395, 404, 602, 619, 871, 1060, 1256, 1432, 1448
HincII GTYRAC 1 cut(s) 1274
HindII GTYRAC 1 cut(s) 1274
HinfI GANTC 7 cut(s) 176, 526, 602, 845, 1224, 1565, 1610
HpaII CCGG 1 cut(s) 878
Hpy166II GTNNAC 3 cut(s) 160, 1274, 1651
Hpy188I TCNGA 8 cut(s) 46, 154, 685, 798, 856, 1107, 1438, 1555
Hpy188III TCNNGA 8 cut(s) 59, 75, 255, 392, 401, 599, 1127, 1382
Hpy8I GTNNAC 3 cut(s) 160, 1274, 1651
Hpy99I CGWCG 2 cut(s) 278, 281
HpyAV CCTTC 4 cut(s) 261, 494, 760, 1597
HpyCH4III ACNGT 6 cut(s) 805, 924, 1134, 1171, 1271, 1716
HpyCH4IV ACGT 1 cut(s) 1470
HpyCH4V TGCA 6 cut(s) 37, 146, 674, 1102, 1174, 1280
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 98
HpyF3I CTNAG 7 cut(s) 117, 474, 493, 560, 684, 744, 1554
HpySE526I ACGT 1 cut(s) 1470
Hsp92II CATG 9 cut(s) 395, 404, 602, 619, 871, 1060, 1256, 1432, 1448
Ksp22I TGATCA 2 cut(s) 388, 397
Kzo9I GATC 6 cut(s) 388, 397, 595, 1297, 1357, 1417
LguI GCTCTTC 2 cut(s) 116, 631
LmnI GCTCC 7 cut(s) 77, 439, 856, 916, 1027, 1168, 1291
Lsp1109I GCAGC 1 cut(s) 637
LweI GCATC 1 cut(s) 1111
MaeI CTAG 5 cut(s) 210, 996, 1076, 1091, 1622
MaeII ACGT 1 cut(s) 1470
MaeIII GTNAC 6 cut(s) 931, 1042, 1265, 1394, 1514, 1577
MalI GATC 6 cut(s) 390, 399, 597, 1299, 1359, 1419
MboI GATC 6 cut(s) 388, 397, 595, 1297, 1357, 1417
MfeI CAATTG 1 cut(s) 458
MhlI GDGCHC 4 cut(s) 861, 913, 1018, 1032
MlyI GAGTC 2 cut(s) 185, 535
MmeI TCCRAC 1 cut(s) 1190
MroXI GAANNNNTTC 1 cut(s) 990
MslI CAYNNNNRTG 2 cut(s) 405, 1401
MspA1I CMGCKG 1 cut(s) 650
MspCI CTTAAG 1 cut(s) 590
MspI CCGG 1 cut(s) 878
MunI CAATTG 1 cut(s) 458
Mva1269I GAATGC 2 cut(s) 744, 1659
MwoI GCNNNNNNNGC 2 cut(s) 29, 98
NdeII GATC 6 cut(s) 388, 397, 595, 1297, 1357, 1417
NlaIII CATG 9 cut(s) 395, 404, 602, 619, 871, 1060, 1256, 1432, 1448
NlaIV GGNNCC 2 cut(s) 858, 1164
NmuCI GTSAC 4 cut(s) 931, 1042, 1394, 1577
NspI RCATGY 1 cut(s) 1256
OliI CACNNNNGTG 1 cut(s) 1401
PaeI GCATGC 1 cut(s) 1256
PagI TCATGA 3 cut(s) 391, 400, 598
PciSI GCTCTTC 2 cut(s) 116, 631
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 342
PctI GAATGC 2 cut(s) 744, 1659
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 5 cut(s) 602, 845, 1224, 1565, 1610
PflFI GACNNNGTC 1 cut(s) 805
PkrI GCNGC 1 cut(s) 652
PleI GAGTC 2 cut(s) 184, 534
PpsI GAGTC 2 cut(s) 184, 534
Psp124BI GAGCTC 1 cut(s) 913
PspEI GGTNACC 1 cut(s) 1042
PspN4I GGNNCC 2 cut(s) 858, 1164
PspPI GGNCC 1 cut(s) 970
PstNI CAGNNNCTG 1 cut(s) 1313
PsyI GACNNNGTC 1 cut(s) 805
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 2 cut(s) 1111, 1180
RsaNI GTAC 2 cut(s) 1110, 1179
RseI CAYNNNNRTG 2 cut(s) 405, 1401
SacI GAGCTC 1 cut(s) 913
SalI GTCGAC 1 cut(s) 1272
SapI GCTCTTC 2 cut(s) 116, 631
SatI GCNGC 1 cut(s) 651
Sau3AI GATC 6 cut(s) 388, 397, 595, 1297, 1357, 1417
Sau96I GGNCC 1 cut(s) 970
SchI GAGTC 2 cut(s) 185, 535
SduI GDGCHC 4 cut(s) 861, 913, 1018, 1032
SfaNI GCATC 1 cut(s) 1111
SfcI CTRYAG 1 cut(s) 1647
SmiMI CAYNNNNRTG 2 cut(s) 405, 1401
SmlI CTYRAG 2 cut(s) 590, 756
SmoI CTYRAG 2 cut(s) 590, 756
SphI GCATGC 1 cut(s) 1256
SsiI CCGC 2 cut(s) 1205, 1607
SspMI CTAG 5 cut(s) 210, 996, 1076, 1091, 1622
SstI GAGCTC 1 cut(s) 913
StyI CCWWGG 2 cut(s) 995, 1399
TaaI ACNGT 6 cut(s) 805, 924, 1134, 1171, 1271, 1716
TaiI ACGT 1 cut(s) 1473
TaqI TCGA 2 cut(s) 234, 1273
TatI WGTACW 2 cut(s) 1109, 1178
TfiI GAWTC 5 cut(s) 602, 845, 1224, 1565, 1610
TscAI CASTG 6 cut(s) 501, 568, 927, 1044, 1176, 1719
TseFI GTSAC 4 cut(s) 931, 1042, 1394, 1577
TseI GCWGC 1 cut(s) 650
Tsp45I GTSAC 4 cut(s) 931, 1042, 1394, 1577
TspDTI ATGAA 5 cut(s) 593, 632, 1424, 1445, 1652
TspGWI ACGGA 1 cut(s) 1290
TspRI CASTG 6 cut(s) 501, 568, 927, 1044, 1176, 1719
Tth111I GACNNNGTC 1 cut(s) 805
Vha464I CTTAAG 1 cut(s) 590
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 3 cut(s) 54, 1432, 1460
XceI RCATGY 1 cut(s) 1256
XmaJI CCTAGG 1 cut(s) 995
XmiI GTMKAC 3 cut(s) 159, 1273, 1650
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 5 cut(s) 210, 996, 1076, 1091, 1622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.