Rh3CG051000

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0017678
Physical Location & Seq
Forward (+)
3459915 .. 3463122
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG051000.1

Sequence Viewer

Length: 1734 bp
ATGGAGAGGAACAAAGGGGAGGCAGTCAGGGCTATGCAACTTTCAGAAATGAAGATTCAAGAGAATGATTTTGCTGGAGCAAGGGAAATGGCACGAATAGCTCACCGACTCTTCCCTGAGGATGAGAACATTTCTAAATTACTTGCACTTTCTGAAGTCTACTATTCAATTTTTGAATCTGGTGAAAGCTCCGAGACCGGAGATGCTGCTAGAGCTGATTTTGAGCAATTTATCGAAAAGGTCAGTAGTCCTTCTCGAAATCTAAATGATGACGATGACGACGATGATAGTGATGATGGTGATGATGATAGTGATGATGATTATGATTATGATGACGATGACGATGACGATGACGACAATGAAGATGATGATTATGATTATGATGGCAACAATGAGTATGATGATCATGACGATGATGATGGTGATGACGATTTTGTGATTGCTCCCAAAAGGTTGAGGGAGAGACAATTGAAGAGTTATACTGAGAAGGAGGAGAAGAATGCAGTGGATGGTGGAGTATCTAAAAGTGTTGTGAGTCGTCCAAAAAGGTTGAGGCACAGCCAATTCCTCAGTGTTATTAACAGAATGCATGACACGTTTAAGGATCATAATTCTGGCAGTTCCATTAAAAGCTCCTCTACCCCTCCTTCGTCATCAGCTGCTCAATTCTATGACTTCAATGCACAAAAGTCTGAGGAGAAGATTAGGGTTGGTCAAATATGGGCGCTGTATGGTGATGGGAATGGAATGTCTAAGACCTTTGCTCAAGTTAAGAGGATTAATGTCAGGCCAAGCAAAAGAGTTCAGAGGACTGTGTCTTCGGGGCATTTTTATTTGCGAATGGGATTGCTTGAACCTTTCTCAGAACCCAAAAACATGGTTGAGCCGGTGTGTTGTGGGACATTTAAAGTCAAACGAGCTCCACCTACACTTTTCTCCCTCACCTCATTATCTCATCGTGTAAAAGCCAGACTTTTGGGCCAGAATATATTTGAAATAATTCCTAGGAGAGGTGAAGTGTGGGCACTATACAAGGAGCACAATCCACTGGTGACCTATCCTAACGTGGGGAAGGGTGAATGTCTAGTGGTGGAAGTGCTGGAAGTTCAGCACCAGAGTACAAAGAGTGTGGTTCTGGAACAGCTTAATGGATTTAAGTCAGTTTTCAAGGCTCCAACAGTGCAGAGTACCAAAACTGGTGTCATTGATATACCGCAGGCTGAGTTGGGTAGATTCTCTCACCAGATTTCTGCTTTTAAGCATACCAAGGTGAGTCACAGTCGACTTGCAGGGTGTTGGGAGCTTGATCCATTATCAGTTTCTGGTTATGAGAAAGATGGTGTTCCCAAATGTTTCTTTAATTGTGATCCTGCTTCTCTGCTTAACAATCCTGACTACTCCTGTGACCTTGGTGATTTGAAAATGGATAAAAGAAGCATGAGTACTGAAACTCATGGTTTGTCTGGTGAGTTACTTGAAGGTGCAGTTAAATCTGTAATAGGTTCTGCGAGCATAGAAACAGGTATGAGACAGGAGACACCTTTTGCTGAAAGAGAGACTTCTGAGAAAGTTGAATCACGAGTTTGTCACTTCTCCTTCTTTGGTAAAGATTTGGTGGATTCCGTTAAGCTAGAAGCAAGTCAATGTGTTAATGCTGTAGACAGTGGCATGCATATGGCAAAAGATGGCAATCTCACTCAACCAAAGGGAAGTGCTATTCCATTGTTGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

577

Amino Acids

64.44

Weight (kDa)

4.87

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 220 - 423 3e-39 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017678)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04682
rosa_chinensis RchiOBHm_Chr3g0453161
rosa_laevigata RLG00000025532
rosa_multiflora Rmu_co8446773.1_g000001 Rmu_sc0007132.1_g000008
rosa_roxburghii Rroxscaffold_6G00430220
rosa_rugosa Rorug02G0646800
rosa_samantha Rh3AG049600 Rh3BG050800 Rh3CG051000 Rh3DG051600
rosa_wichuraiana Rw3G003810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 159, 1282, 1659
AciI CCGC 1 cut(s) 1214
AclWI GGATC 3 cut(s) 612, 1301, 1361
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 3 cut(s) 1120, 1189, 1444
AfiI CCNNNNNNNGG 3 cut(s) 1010, 1067, 1727
AflIII ACRYGT 1 cut(s) 594
AluBI AGCT 9 cut(s) 101, 189, 215, 633, 659, 920, 1144, 1303, 1630
AluI AGCT 9 cut(s) 101, 189, 215, 633, 659, 920, 1144, 1303, 1630
Alw21I GWGCWC 2 cut(s) 922, 1041
Alw26I GTCTC 5 cut(s) 188, 457, 1522, 1529, 1550
AlwI GGATC 3 cut(s) 612, 1301, 1361
AlwNI CAGNNNCTG 1 cut(s) 1322
AoxI GGCC 2 cut(s) 788, 979
ApeKI GCWGC 2 cut(s) 206, 659
ArsI GACNNNNNNTTYG 4 cut(s) 802, 834, 1185, 1217
AseI ATTAAT 1 cut(s) 780
Asp700I GAANNNNTTC 1 cut(s) 999
AspA2I CCTAGG 1 cut(s) 1004
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 1 cut(s) 979
AvrII CCTAGG 1 cut(s) 1004
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 1027
BanII GRGCYC 1 cut(s) 922
BarI GAAGNNNNNNTAC 2 cut(s) 1426, 1458
BauI CACGAG 1 cut(s) 1578
BbsI GAAGAC 1 cut(s) 810
Bbv12I GWGCWC 2 cut(s) 922, 1041
BbvI GCAGC 2 cut(s) 193, 646
BccI CCATC 7 cut(s) 290, 377, 413, 503, 731, 1331, 1679
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 5 cut(s) 188, 457, 1522, 1529, 1550
BfaI CTAG 4 cut(s) 210, 1005, 1085, 1631
BfmI CTRYAG 1 cut(s) 1656
BfoI RGCGCY 1 cut(s) 728
BisI GCNGC 2 cut(s) 207, 660
BlnI CCTAGG 1 cut(s) 1004
BlsI GCNGC 2 cut(s) 208, 661
BmcAI AGTACT 1 cut(s) 1444
BmgT120I GGNCC 1 cut(s) 979
BmiI GGNNCC 1 cut(s) 1173
BmsI GCATC 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 810
BpmI CTGGAG 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 750
BsaBI GATNNNNATC 1 cut(s) 1689
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 1004, 1266, 1408
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 3 cut(s) 1010, 1067, 1727
Bse118I RCCGGY 1 cut(s) 886
Bse1I ACTGG 2 cut(s) 1053, 1201
Bse21I CCTNAGG 1 cut(s) 117
Bse8I GATNNNNATC 1 cut(s) 1689
BseDI CCNNGG 3 cut(s) 1004, 1266, 1408
BseGI GGATG 2 cut(s) 127, 514
BseJI GATNNNNATC 1 cut(s) 1689
BseLI CCNNNNNNNGG 3 cut(s) 1010, 1067, 1727
BseMII CTCAG 7 cut(s) 108, 474, 583, 684, 876, 1212, 1554
BseNI ACTGG 2 cut(s) 1053, 1201
BseRI GAGGAG 3 cut(s) 506, 625, 710
BseSI GKGCMC 1 cut(s) 1027
BseXI GCAGC 2 cut(s) 193, 646
BsgI GTGCAG 2 cut(s) 1202, 1503
BshFI GGCC 2 cut(s) 790, 981
BsiHKAI GWGCWC 2 cut(s) 922, 1041
BsiSI CCGG 2 cut(s) 198, 887
BslFI GGGAC 1 cut(s) 913
BslI CCNNNNNNNGG 3 cut(s) 1010, 1067, 1727
BsmAI GTCTC 5 cut(s) 188, 457, 1522, 1529, 1550
BsmFI GGGAC 1 cut(s) 913
BsmI GAATGC 2 cut(s) 505, 591
BsnI GGCC 2 cut(s) 790, 981
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 3 cut(s) 922, 1027, 1041
Bsp143I GATC 4 cut(s) 403, 604, 1306, 1366
BspACI CCGC 1 cut(s) 1214
BspANI GGCC 2 cut(s) 790, 981
BspCNI CTCAG 7 cut(s) 109, 475, 582, 685, 875, 1213, 1555
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 1 cut(s) 1173
BspPI GGATC 3 cut(s) 612, 1301, 1361
BspTNI GGTCTC 1 cut(s) 188
BsrFI RCCGGY 1 cut(s) 886
BsrI ACTGG 2 cut(s) 1053, 1201
BssAI RCCGGY 1 cut(s) 886
BssECI CCNNGG 3 cut(s) 1004, 1266, 1408
BssMI GATC 4 cut(s) 403, 604, 1306, 1366
BssSI CACGAG 1 cut(s) 1578
BssT1I CCWWGG 3 cut(s) 1004, 1266, 1408
Bst2BI CACGAG 1 cut(s) 1578
Bst4CI ACNGT 4 cut(s) 814, 1180, 1280, 1664
Bst6I CTCTTC 2 cut(s) 116, 467
BstC8I GCNNGC 3 cut(s) 1218, 1510, 1670
BstDEI CTNAG 8 cut(s) 117, 483, 569, 693, 753, 862, 1221, 1563
BstEII GGTNACC 1 cut(s) 1051
BstENI CCTNNNNNAGG 1 cut(s) 1008
BstF5I GGATG 2 cut(s) 127, 514
BstH2I RGCGCY 1 cut(s) 728
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 4 cut(s) 406, 607, 1309, 1369
BstMAI GTCTC 5 cut(s) 188, 457, 1522, 1529, 1550
BstMBI GATC 4 cut(s) 403, 604, 1306, 1366
BstMWI GCNNNNNNNGC 3 cut(s) 29, 98, 212
BstNSI RCATGY 1 cut(s) 1672
BstPI GGTNACC 1 cut(s) 1051
BstSFI CTRYAG 1 cut(s) 1656
BstSLI GKGCMC 1 cut(s) 1027
BstV1I GCAGC 2 cut(s) 193, 646
BstV2I GAAGAC 1 cut(s) 810
BstXI CCANNNNNNTGG 2 cut(s) 877, 976
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 2 cut(s) 790, 981
BtsCI GGATG 2 cut(s) 127, 514
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 5 cut(s) 510, 577, 1046, 1185, 1669
Cac8I GCNNGC 3 cut(s) 1218, 1510, 1670
CaiI CAGNNNCTG 1 cut(s) 1322
CciI TCATGA 1 cut(s) 406
CfoI GCGC 1 cut(s) 727
Cfr10I RCCGGY 1 cut(s) 886
Cfr13I GGNCC 1 cut(s) 979
Csp6I GTAC 3 cut(s) 1119, 1188, 1443
CspCI CAANNNNNGTGG 2 cut(s) 1110, 1145
CviAII CATG 6 cut(s) 407, 590, 877, 1438, 1454, 1669
CviQI GTAC 3 cut(s) 1119, 1188, 1443
DdeI CTNAG 8 cut(s) 117, 483, 569, 693, 753, 862, 1221, 1563
DpnI GATC 4 cut(s) 405, 606, 1308, 1368
DpnII GATC 4 cut(s) 403, 604, 1306, 1366
DraI TTTAAA 1 cut(s) 907
Eam1104I CTCTTC 2 cut(s) 116, 467
EarI CTCTTC 2 cut(s) 116, 467
Ecl136II GAGCTC 1 cut(s) 920
Eco130I CCWWGG 3 cut(s) 1004, 1266, 1408
Eco24I GRGCYC 1 cut(s) 922
Eco31I GGTCTC 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 920
Eco57I CTGAAG 1 cut(s) 174
Eco81I CCTNAGG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 1051
EcoICRI GAGCTC 1 cut(s) 920
EcoNI CCTNNNNNAGG 1 cut(s) 1008
EcoO65I GGTNACC 1 cut(s) 1051
EcoT14I CCWWGG 3 cut(s) 1004, 1266, 1408
EcoT22I ATGCAT 2 cut(s) 591, 1674
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 3 cut(s) 1004, 1266, 1408
FaeI CATG 6 cut(s) 410, 593, 880, 1441, 1457, 1672
FalI AAGNNNNNCTT 4 cut(s) 957, 989, 1543, 1575
FaqI GGGAC 1 cut(s) 913
FatI CATG 6 cut(s) 406, 589, 876, 1437, 1453, 1668
FauNDI CATATG 1 cut(s) 1674
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 3 cut(s) 159, 1282, 1659
Fnu4HI GCNGC 2 cut(s) 207, 660
FokI GGATG 2 cut(s) 134, 521
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 2 cut(s) 207, 660
FspBI CTAG 4 cut(s) 210, 1005, 1085, 1631
GlaI GCGC 1 cut(s) 726
GluI GCNGC 2 cut(s) 207, 660
GsuI CTGGAG 1 cut(s) 96
HaeII RGCGCY 1 cut(s) 728
HaeIII GGCC 2 cut(s) 790, 981
HapII CCGG 2 cut(s) 198, 887
HhaI GCGC 1 cut(s) 727
Hin1II CATG 6 cut(s) 410, 593, 880, 1441, 1457, 1672
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HincII GTYRAC 1 cut(s) 1283
HindII GTYRAC 1 cut(s) 1283
HinfI GANTC 8 cut(s) 55, 108, 176, 535, 1233, 1273, 1574, 1619
HpaII CCGG 2 cut(s) 198, 887
Hpy166II GTNNAC 3 cut(s) 160, 1283, 1660
Hpy188I TCNGA 7 cut(s) 46, 154, 193, 694, 807, 865, 1564
Hpy188III TCNNGA 6 cut(s) 59, 255, 407, 1136, 1391, 1578
Hpy8I GTNNAC 3 cut(s) 160, 1283, 1660
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 6 cut(s) 261, 481, 657, 1066, 1472, 1606
HpyCH4III ACNGT 4 cut(s) 814, 1180, 1280, 1664
HpyCH4IV ACGT 2 cut(s) 596, 1065
HpyCH4V TGCA 9 cut(s) 37, 146, 503, 589, 683, 1183, 1289, 1484, 1672
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 98, 212
HpyF3I CTNAG 8 cut(s) 117, 483, 569, 693, 753, 862, 1221, 1563
HpySE526I ACGT 2 cut(s) 596, 1065
Hsp92II CATG 6 cut(s) 410, 593, 880, 1441, 1457, 1672
HspAI GCGC 1 cut(s) 725
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 4 cut(s) 403, 604, 1306, 1366
LmnI GCTCC 8 cut(s) 77, 194, 448, 638, 925, 1036, 1177, 1300
Lsp1109I GCAGC 2 cut(s) 193, 646
LweI GCATC 1 cut(s) 193
MaeI CTAG 4 cut(s) 210, 1005, 1085, 1631
MaeII ACGT 2 cut(s) 596, 1065
MaeIII GTNAC 5 cut(s) 1051, 1274, 1403, 1470, 1586
MalI GATC 4 cut(s) 405, 606, 1308, 1368
MboI GATC 4 cut(s) 403, 604, 1306, 1366
MboII GAAGA 7 cut(s) 64, 103, 374, 484, 508, 712, 810
MfeI CAATTG 1 cut(s) 467
MhlI GDGCHC 3 cut(s) 922, 1027, 1041
MluCI AATT 9 cut(s) 137, 168, 227, 467, 563, 610, 665, 999, 1360
MlyI GAGTC 3 cut(s) 102, 544, 1282
MmeI TCCRAC 1 cut(s) 1199
Mph1103I ATGCAT 2 cut(s) 591, 1674
MroXI GAANNNNTTC 1 cut(s) 999
MslI CAYNNNNRTG 2 cut(s) 411, 1673
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 2 cut(s) 198, 887
MunI CAATTG 1 cut(s) 467
Mva1269I GAATGC 2 cut(s) 505, 591
MwoI GCNNNNNNNGC 3 cut(s) 29, 98, 212
NdeI CATATG 1 cut(s) 1674
NdeII GATC 4 cut(s) 403, 604, 1306, 1366
NlaIII CATG 6 cut(s) 410, 593, 880, 1441, 1457, 1672
NlaIV GGNNCC 1 cut(s) 1173
NmuCI GTSAC 4 cut(s) 1051, 1274, 1403, 1586
NsiI ATGCAT 2 cut(s) 591, 1674
NspI RCATGY 1 cut(s) 1672
PaeI GCATGC 1 cut(s) 1672
PagI TCATGA 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PctI GAATGC 2 cut(s) 505, 591
PdmI GAANNNNTTC 1 cut(s) 999
PfeI GAWTC 5 cut(s) 55, 176, 1233, 1574, 1619
PflFI GACNNNGTC 1 cut(s) 814
PkrI GCNGC 2 cut(s) 208, 661
PleI GAGTC 3 cut(s) 102, 543, 1281
PpsI GAGTC 3 cut(s) 102, 543, 1281
PshBI ATTAAT 1 cut(s) 780
Psp124BI GAGCTC 1 cut(s) 922
PspEI GGTNACC 1 cut(s) 1051
PspN4I GGNNCC 1 cut(s) 1173
PspPI GGNCC 1 cut(s) 979
PstNI CAGNNNCTG 1 cut(s) 1322
PsyI GACNNNGTC 1 cut(s) 814
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 3 cut(s) 1120, 1189, 1444
RsaNI GTAC 3 cut(s) 1119, 1188, 1443
RseI CAYNNNNRTG 2 cut(s) 411, 1673
SacI GAGCTC 1 cut(s) 922
SalI GTCGAC 1 cut(s) 1281
SatI GCNGC 2 cut(s) 207, 660
Sau3AI GATC 4 cut(s) 403, 604, 1306, 1366
Sau96I GGNCC 1 cut(s) 979
ScaI AGTACT 1 cut(s) 1444
SchI GAGTC 3 cut(s) 102, 544, 1282
SduI GDGCHC 3 cut(s) 922, 1027, 1041
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 1656
SmiMI CAYNNNNRTG 2 cut(s) 411, 1673
SmlI CTYRAG 1 cut(s) 765
SmoI CTYRAG 1 cut(s) 765
SphI GCATGC 1 cut(s) 1672
Sse9I AATT 9 cut(s) 137, 168, 227, 467, 563, 610, 665, 999, 1360
SsiI CCGC 1 cut(s) 1214
SspMI CTAG 4 cut(s) 210, 1005, 1085, 1631
SstI GAGCTC 1 cut(s) 922
StyI CCWWGG 3 cut(s) 1004, 1266, 1408
TaaI ACNGT 4 cut(s) 814, 1180, 1280, 1664
TaiI ACGT 2 cut(s) 599, 1068
TaqI TCGA 3 cut(s) 234, 256, 1282
TasI AATT 9 cut(s) 137, 168, 227, 467, 563, 610, 665, 999, 1360
TatI WGTACW 2 cut(s) 1118, 1442
TfiI GAWTC 5 cut(s) 55, 176, 1233, 1574, 1619
TscAI CASTG 5 cut(s) 510, 577, 1053, 1185, 1669
TseFI GTSAC 4 cut(s) 1051, 1274, 1403, 1586
TseI GCWGC 2 cut(s) 206, 659
Tsp45I GTSAC 4 cut(s) 1051, 1274, 1403, 1586
TspDTI ATGAA 2 cut(s) 65, 375
TspGWI ACGGA 1 cut(s) 1612
TspRI CASTG 5 cut(s) 510, 577, 1053, 1185, 1669
Tth111I GACNNNGTC 1 cut(s) 814
VspI ATTAAT 1 cut(s) 780
XagI CCTNNNNNAGG 1 cut(s) 1008
XceI RCATGY 1 cut(s) 1672
XmaJI CCTAGG 1 cut(s) 1004
XmiI GTMKAC 3 cut(s) 159, 1282, 1659
XmnI GAANNNNTTC 1 cut(s) 999
XspI CTAG 4 cut(s) 210, 1005, 1085, 1631
ZrmI AGTACT 1 cut(s) 1444
Zsp2I ATGCAT 2 cut(s) 591, 1674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.