Rroxscaffold_6G00430220

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017678
Physical Location & Seq
Forward (+)
50396851 .. 50400149
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430220.1

Sequence Viewer

Length: 1731 bp
ATGGAGAGGAACAAAGGGGAGGCAGTCAGGGCAATGCAACTTTCAGAAATGAAGATTCAAGAGAATGATTTTGCTGGAGCAAGGGAAATGGCACGAATAGCTCACCGACTCTTCCCTGAGGATGAGAACATTTCTAAATTACTTGCACTCTCTGAAGTCTACTATTCAATTTTTGAATCTGGTGAAAGCTCCGAGACCGGAGATACTGCTAGAGCTGATTTTGAGCAATTTATCGAAAAGGTCAGTAGTCCTTCTGGAAATCTAAATGATGATGACGACGATGATGATAGTGATGATGGTGATGATGATAGTGATGATGATTATGATTATGATGATGATGACGACGACGATGAAGATGAAGATGATGATTATGATTATGATGGCAACAATGAGTATGATGATCATGATGATGATGATGGTGATGACGATTTTGTGATTGCTCCCAAAAGGTTGAGGGAGAGACAATTGAAGGGTTATACTGAGAAGGAGGAGAAGAATGCAGTGGATGGTGGAGTATCTAAAAGTGTTGTGAGTCGTCCAAAAAGGTTGAGGCACAGCCAATTCCTCAGTGTTATTAACAGAATGCATGACACGTTTAAGGATCATAATTCTATCAGTTCCATTAAAAGCTCCTCTACCCCTCCATCGTCATCAGCTGTTCAATTCTATGACTTCAATGCACAAAAGTCTGAGGAGAAGATTAGGGTTGGTCAAATATGGGCACTGTATAGTGATGGGAATGGAATGTCTAAGACCTTTGCTCAAGTTAAGAGGATTAATGTCAGGCCAAACAAAAGAGCTCAGAGGACTGTGTCTTCGGGCCATTTTTATTTGCGAATGGGATTGCTTGAACCTTTCTTGGAACCCAAAAACATGGTTGAGCCGGTGTGTTGTGGGACATTTAAAGTCAAACGAGCTCCACCTACACTGTTCTCCCTCACCTCATTATCTCATCGTGTAAAAGCCAGACTTTTGGGCCAGAATATATTTGAAATAATTCCTAGGAGAGGTGAAGTTTGGGCACTATACAAGGAGCACAATTCACTGATGACCTATCCTAACATGGGGAAGGGTGAATGTCTAGTGGTGGAAGTGCTGGAAGTTGAGCACCAGAGTACAAAGATTGTGGTTCTGGAACAGCTTAATGGATTTAAGTCAGTTTTCAAGGCTCCAACAGTGCAGAGTACCAAAACTGGTGTCATTGATTTACCGCAGGCTGAGTTGGGTAGATTCTCTCACCAGATTTCTGCTTTTAAGCATACCAAGGTGAGTCACAGTCGACTTGCAGGGTGTTGGGAGCTTGATCCATTATCAGTTTCTGGTTATGAGAAGGATGGTGTTCCCAAATGTTTCTTTAATTGTGATCCTGCTTCTCTGCTGAACAATCCTGACTACTCCCGTTACCTTGGTGATTTGAAAATGGATAAAAGAAGCATGAATACTGAAACTCATGGTTTGTCTGGTGAGTTACTTGAAGGTGCAGCTAAATCTGTAATAGGTTCTGCAAGCATAGAAACAGGTATGAAACAGGAGACACCTTTTGCTGAAAGAGACACTTCTGAGAAAGTTGAATCACGAGTTTGTCACTTCTCCTTCTTTGGTAAAGATTTGGTGGATTCCGTTAAGCTAGAAGCAAGTCAATGTGTTAATGCTGTAGACAATGGCATGCATATCGCAAAAGATGGCAATCTCACTCAACCAAAGGGAAGTGCTATTCCATTGTTGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

576

Amino Acids

64.53

Weight (kDa)

4.87

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 219 - 422 2.6e-39 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017678)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04682
rosa_chinensis RchiOBHm_Chr3g0453161
rosa_laevigata RLG00000025532
rosa_multiflora Rmu_co8446773.1_g000001 Rmu_sc0007132.1_g000008
rosa_roxburghii Rroxscaffold_6G00430220
rosa_rugosa Rorug02G0646800
rosa_samantha Rh3AG049600 Rh3BG050800 Rh3CG051000 Rh3DG051600
rosa_wichuraiana Rw3G003810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 159, 1279, 1656
AciI CCGC 1 cut(s) 1211
AclWI GGATC 3 cut(s) 609, 1298, 1358
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 2 cut(s) 1117, 1186
AfiI CCNNNNNNNGG 3 cut(s) 1007, 1064, 1724
AflIII ACRYGT 1 cut(s) 591
Alw21I GWGCWC 4 cut(s) 802, 919, 1038, 1110
Alw26I GTCTC 4 cut(s) 188, 454, 1526, 1545
AlwI GGATC 3 cut(s) 609, 1298, 1358
AlwNI CAGNNNCTG 1 cut(s) 1319
AoxI GGCC 3 cut(s) 785, 820, 976
ApeKI GCWGC 1 cut(s) 1481
ArsI GACNNNNNNTTYG 4 cut(s) 799, 831, 1182, 1214
AseI ATTAAT 1 cut(s) 777
Asp700I GAANNNNTTC 1 cut(s) 996
AspA2I CCTAGG 1 cut(s) 1001
AspS9I GGNCC 2 cut(s) 820, 976
AvrII CCTAGG 1 cut(s) 1001
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 2 cut(s) 724, 1024
BanII GRGCYC 2 cut(s) 802, 919
BarI GAAGNNNNNNTAC 2 cut(s) 1423, 1455
BauI CACGAG 1 cut(s) 1575
BbsI GAAGAC 1 cut(s) 807
Bbv12I GWGCWC 4 cut(s) 802, 919, 1038, 1110
BbvI GCAGC 1 cut(s) 1493
BccI CCATC 8 cut(s) 290, 374, 410, 500, 652, 728, 1328, 1676
BcgI CGANNNNNNTGC 2 cut(s) 1654, 1688
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 4 cut(s) 188, 454, 1526, 1545
BfaI CTAG 4 cut(s) 210, 1002, 1082, 1628
BfmI CTRYAG 1 cut(s) 1653
BisI GCNGC 1 cut(s) 1482
BlnI CCTAGG 1 cut(s) 1001
BlsI GCNGC 1 cut(s) 1483
BmgT120I GGNCC 2 cut(s) 820, 976
BmiI GGNNCC 2 cut(s) 864, 1170
BpiI GAAGAC 1 cut(s) 807
BpmI CTGGAG 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 747
BsaBI GATNNNNATC 1 cut(s) 1686
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 1001, 1263, 1405
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 3 cut(s) 1007, 1064, 1724
Bse118I RCCGGY 1 cut(s) 883
Bse1I ACTGG 1 cut(s) 1198
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 39
Bse8I GATNNNNATC 1 cut(s) 1686
BseDI CCNNGG 3 cut(s) 1001, 1263, 1405
BseGI GGATG 3 cut(s) 127, 511, 1339
BseJI GATNNNNATC 1 cut(s) 1686
BseLI CCNNNNNNNGG 3 cut(s) 1007, 1064, 1724
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 7 cut(s) 108, 471, 580, 681, 815, 1209, 1551
BseNI ACTGG 1 cut(s) 1198
BseRI GAGGAG 3 cut(s) 503, 622, 707
BseSI GKGCMC 2 cut(s) 724, 1024
BseXI GCAGC 1 cut(s) 1493
BsgI GTGCAG 2 cut(s) 1199, 1500
BshFI GGCC 3 cut(s) 787, 822, 978
BsiHKAI GWGCWC 4 cut(s) 802, 919, 1038, 1110
BsiSI CCGG 2 cut(s) 198, 884
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 3 cut(s) 1007, 1064, 1724
BsmAI GTCTC 4 cut(s) 188, 454, 1526, 1545
BsmFI GGGAC 1 cut(s) 910
BsmI GAATGC 2 cut(s) 502, 588
BsnI GGCC 3 cut(s) 787, 822, 978
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 6 cut(s) 724, 802, 919, 1024, 1038, 1110
Bsp143I GATC 4 cut(s) 400, 601, 1303, 1363
BspACI CCGC 1 cut(s) 1211
BspANI GGCC 3 cut(s) 787, 822, 978
BspCNI CTCAG 7 cut(s) 109, 472, 579, 682, 814, 1210, 1552
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 864, 1170
BspPI GGATC 3 cut(s) 609, 1298, 1358
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 883
BsrI ACTGG 1 cut(s) 1198
BssAI RCCGGY 1 cut(s) 883
BssECI CCNNGG 3 cut(s) 1001, 1263, 1405
BssMI GATC 4 cut(s) 400, 601, 1303, 1363
BssSI CACGAG 1 cut(s) 1575
BssT1I CCWWGG 3 cut(s) 1001, 1263, 1405
Bst2BI CACGAG 1 cut(s) 1575
Bst4CI ACNGT 5 cut(s) 726, 811, 930, 1177, 1277
Bst6I CTCTTC 1 cut(s) 116
BstC8I GCNNGC 3 cut(s) 1215, 1507, 1667
BstDEI CTNAG 8 cut(s) 117, 480, 566, 690, 750, 801, 1218, 1560
BstENI CCTNNNNNAGG 1 cut(s) 1005
BstF5I GGATG 3 cut(s) 127, 511, 1339
BstKTI GATC 4 cut(s) 403, 604, 1306, 1366
BstMAI GTCTC 4 cut(s) 188, 454, 1526, 1545
BstMBI GATC 4 cut(s) 400, 601, 1303, 1363
BstMWI GCNNNNNNNGC 2 cut(s) 29, 98
BstNSI RCATGY 1 cut(s) 1669
BstSFI CTRYAG 1 cut(s) 1653
BstSLI GKGCMC 2 cut(s) 724, 1024
BstV1I GCAGC 1 cut(s) 1493
BstV2I GAAGAC 1 cut(s) 807
BstXI CCANNNNNNTGG 2 cut(s) 874, 973
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 3 cut(s) 787, 822, 978
BtsCI GGATG 3 cut(s) 127, 511, 1339
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 6 cut(s) 507, 574, 722, 926, 1043, 1182
Cac8I GCNNGC 3 cut(s) 1215, 1507, 1667
CaiI CAGNNNCTG 1 cut(s) 1319
CciI TCATGA 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 883
Cfr13I GGNCC 2 cut(s) 820, 976
Csp6I GTAC 2 cut(s) 1116, 1185
CspCI CAANNNNNGTGG 2 cut(s) 1107, 1142
CviAII CATG 7 cut(s) 404, 587, 874, 1063, 1435, 1451, 1666
CviQI GTAC 2 cut(s) 1116, 1185
DdeI CTNAG 8 cut(s) 117, 480, 566, 690, 750, 801, 1218, 1560
DpnI GATC 4 cut(s) 402, 603, 1305, 1365
DpnII GATC 4 cut(s) 400, 601, 1303, 1363
DraI TTTAAA 1 cut(s) 904
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Ecl136II GAGCTC 2 cut(s) 800, 917
Eco130I CCWWGG 3 cut(s) 1001, 1263, 1405
Eco24I GRGCYC 2 cut(s) 802, 919
Eco31I GGTCTC 1 cut(s) 188
Eco53kI GAGCTC 2 cut(s) 800, 917
Eco57I CTGAAG 1 cut(s) 174
Eco81I CCTNAGG 1 cut(s) 117
EcoICRI GAGCTC 2 cut(s) 800, 917
EcoNI CCTNNNNNAGG 1 cut(s) 1005
EcoT14I CCWWGG 3 cut(s) 1001, 1263, 1405
EcoT22I ATGCAT 2 cut(s) 588, 1671
EcoT38I GRGCYC 2 cut(s) 802, 919
ErhI CCWWGG 3 cut(s) 1001, 1263, 1405
FaeI CATG 7 cut(s) 407, 590, 877, 1066, 1438, 1454, 1669
FalI AAGNNNNNCTT 4 cut(s) 954, 986, 1540, 1572
FaqI GGGAC 1 cut(s) 910
FatI CATG 7 cut(s) 403, 586, 873, 1062, 1434, 1450, 1665
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 3 cut(s) 159, 1279, 1656
Fnu4HI GCNGC 1 cut(s) 1482
FokI GGATG 3 cut(s) 134, 518, 1346
FriOI GRGCYC 2 cut(s) 802, 919
Fsp4HI GCNGC 1 cut(s) 1482
FspBI CTAG 4 cut(s) 210, 1002, 1082, 1628
GluI GCNGC 1 cut(s) 1482
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 3 cut(s) 787, 822, 978
HapII CCGG 2 cut(s) 198, 884
Hin1II CATG 7 cut(s) 407, 590, 877, 1066, 1438, 1454, 1669
HincII GTYRAC 1 cut(s) 1280
HindII GTYRAC 1 cut(s) 1280
HinfI GANTC 8 cut(s) 55, 108, 176, 532, 1230, 1270, 1571, 1616
HpaII CCGG 2 cut(s) 198, 884
Hpy166II GTNNAC 3 cut(s) 160, 1280, 1657
Hpy188I TCNGA 6 cut(s) 46, 154, 193, 691, 804, 1561
Hpy188III TCNNGA 6 cut(s) 59, 255, 404, 1133, 1388, 1575
Hpy8I GTNNAC 3 cut(s) 160, 1280, 1657
Hpy99I CGWCG 3 cut(s) 281, 347, 350
HpyAV CCTTC 7 cut(s) 261, 463, 478, 1063, 1324, 1469, 1603
HpyCH4III ACNGT 5 cut(s) 726, 811, 930, 1177, 1277
HpyCH4IV ACGT 1 cut(s) 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 98
HpyF3I CTNAG 8 cut(s) 117, 480, 566, 690, 750, 801, 1218, 1560
HpySE526I ACGT 1 cut(s) 593
Hsp92II CATG 7 cut(s) 407, 590, 877, 1066, 1438, 1454, 1669
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 4 cut(s) 400, 601, 1303, 1363
LmnI GCTCC 8 cut(s) 77, 194, 445, 635, 922, 1033, 1174, 1297
Lsp1109I GCAGC 1 cut(s) 1493
MaeI CTAG 4 cut(s) 210, 1002, 1082, 1628
MaeII ACGT 1 cut(s) 593
MaeIII GTNAC 4 cut(s) 1271, 1400, 1467, 1583
MalI GATC 4 cut(s) 402, 603, 1305, 1365
MboI GATC 4 cut(s) 400, 601, 1303, 1363
MboII GAAGA 7 cut(s) 64, 103, 365, 371, 505, 709, 807
MfeI CAATTG 1 cut(s) 464
MhlI GDGCHC 6 cut(s) 724, 802, 919, 1024, 1038, 1110
MlyI GAGTC 3 cut(s) 102, 541, 1279
MmeI TCCRAC 1 cut(s) 1196
Mph1103I ATGCAT 2 cut(s) 588, 1671
MroXI GAANNNNTTC 1 cut(s) 996
MslI CAYNNNNRTG 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 2 cut(s) 198, 884
MunI CAATTG 1 cut(s) 464
Mva1269I GAATGC 2 cut(s) 502, 588
MwoI GCNNNNNNNGC 2 cut(s) 29, 98
NdeII GATC 4 cut(s) 400, 601, 1303, 1363
NlaIII CATG 7 cut(s) 407, 590, 877, 1066, 1438, 1454, 1669
NlaIV GGNNCC 2 cut(s) 864, 1170
NmuCI GTSAC 2 cut(s) 1271, 1583
NsiI ATGCAT 2 cut(s) 588, 1671
NspI RCATGY 1 cut(s) 1669
PaeI GCATGC 1 cut(s) 1669
PagI TCATGA 1 cut(s) 403
PctI GAATGC 2 cut(s) 502, 588
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 5 cut(s) 55, 176, 1230, 1571, 1616
PflFI GACNNNGTC 1 cut(s) 811
PkrI GCNGC 1 cut(s) 1483
PleI GAGTC 3 cut(s) 102, 540, 1278
PpsI GAGTC 3 cut(s) 102, 540, 1278
PshBI ATTAAT 1 cut(s) 777
Psp124BI GAGCTC 2 cut(s) 802, 919
PspN4I GGNNCC 2 cut(s) 864, 1170
PspPI GGNCC 2 cut(s) 820, 976
PstNI CAGNNNCTG 1 cut(s) 1319
PsyI GACNNNGTC 1 cut(s) 811
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 2 cut(s) 1117, 1186
RsaNI GTAC 2 cut(s) 1116, 1185
RseI CAYNNNNRTG 1 cut(s) 408
SacI GAGCTC 2 cut(s) 802, 919
SalI GTCGAC 1 cut(s) 1278
SatI GCNGC 1 cut(s) 1482
Sau3AI GATC 4 cut(s) 400, 601, 1303, 1363
Sau96I GGNCC 2 cut(s) 820, 976
SchI GAGTC 3 cut(s) 102, 541, 1279
SduI GDGCHC 6 cut(s) 724, 802, 919, 1024, 1038, 1110
SfcI CTRYAG 1 cut(s) 1653
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
SphI GCATGC 1 cut(s) 1669
SsiI CCGC 1 cut(s) 1211
SspMI CTAG 4 cut(s) 210, 1002, 1082, 1628
SstI GAGCTC 2 cut(s) 802, 919
StyI CCWWGG 3 cut(s) 1001, 1263, 1405
TaaI ACNGT 5 cut(s) 726, 811, 930, 1177, 1277
TaiI ACGT 1 cut(s) 596
TaqI TCGA 2 cut(s) 234, 1279
TatI WGTACW 1 cut(s) 1115
TfiI GAWTC 5 cut(s) 55, 176, 1230, 1571, 1616
TscAI CASTG 6 cut(s) 507, 574, 729, 933, 1050, 1182
TseFI GTSAC 2 cut(s) 1271, 1583
TseI GCWGC 1 cut(s) 1481
Tsp45I GTSAC 2 cut(s) 1271, 1583
TspDTI ATGAA 5 cut(s) 65, 366, 372, 1451, 1538
TspGWI ACGGA 1 cut(s) 1609
TspRI CASTG 6 cut(s) 507, 574, 729, 933, 1050, 1182
Tth111I GACNNNGTC 1 cut(s) 811
VspI ATTAAT 1 cut(s) 777
XagI CCTNNNNNAGG 1 cut(s) 1005
XceI RCATGY 1 cut(s) 1669
XmaJI CCTAGG 1 cut(s) 1001
XmiI GTMKAC 3 cut(s) 159, 1279, 1656
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 4 cut(s) 210, 1002, 1082, 1628
Zsp2I ATGCAT 2 cut(s) 588, 1671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.