RchiOBHm_Chr3g0453161

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0017678
Physical Location & Seq
Forward (+)
3663523 .. 3666721
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42027

Sequence Viewer

Length: 1737 bp
ATGGAGAGGAACAAAGGGGAGGCAGTCAGGGCTATGCAACTTTCAGAAATGAAGATTCAAGAGAATGATTTTGCTGGAGCAAGGGAAATGGCACGAATAGCTCACCGACTCTACCCTGAGGATGAGAACATTTCTAAATTACTTGCACTCTCTGAAGTCTACTATTCAATTTTTGAATCTGGTGAAAGCTCCGAGACTGGAGATACTGCTAGAGATGATTTTGAGCAATTTATCGAAAAGGTCAGTAGTCCTTCTGGAAATCTAAATGATGATGACGACGATGATGATAGTGATGATGGTGATGATGATAGTGATGATGATTATGATTATGATGACGATGACGACGACGACGATGACGACAATGAAGATGATGATTATGATTATGATGGCAACAATGAGTATGATGATCATGATGATGATGATGGTGATGACGATTTTGTGATTGCTCCCAAAAGGTTGAGGGAGAGACAATTGAAGAGTTATACTGAGAAGGAGGAGAAGAATGCAGTGGATGGTGGAGTATCTAAAAGTGTTGTGAGTCGTCCAAAAAGGTTGAGGCACAGCCAATTCCTCAGTGTTATTAACAGAATGCATGACACGTTTAAGGATCATAATTCTGGCAGTTCCATTAAAAGCTCCTCTACCCCTCCATCGTCATCAGCTGCTCAATTCTATGACTTCAATGCACAAAAGTCAGAGGAGAAGATTAGGGTTGGTCAAATATGGGCGCTGTATGGTGATGGGAATGGAATGTCTAAGACCTTTGCTCAAGTTAAGAGGATTAATGTCAGGCCAAACAAAAGAGTTCAGAGGACTGTGTCTTCGGGGCATTTTTATTTGCGAATGGGATTGCTTGAACCTTTCTTGGAACCCAAAAACATGGTTGAGCCAGTGTGTTGTGGGACATTTAAAGTCAAACGAGCTCCACCTACACTTTTCTCCCTCACTTCATTATCTCATCGTGTAAAAGCCAGACTTTTGGGCCAGAATATATTTGAAATAATTCCTAGGAGAGGTGAAGTGTGGGCACTATACAAGGAGCACAATCCACTGGTGACCTATCCTAACATGGGGAAGGGTGAATGTCTAGTGGTGGAAGTGCTGGAAGTTCAGCACCAGAGTACAAAGATTGTGGTTCTGGAACAGCTTAATGCATTTAAGTCAGTTTTCAAGGCTCCAACAGTGCAGTGTACCAAAACTGGTGTCATTGATATACCGCAGGCTGAGTTGGGTAGATTCTCTCACCAGATTTCTGCTTTTAAGCATACCAAGGTGAGTCACAGTCGACTTGCAGGGTGTTGGGAGCTTGATCCATTATCAGTTTCTGGTTATGAGAAAGATGGTGTTCCCAAATGTTTCTTTAATTGTGATCCTGCTTCTCTGCTTAACAATCCTGACTACTCCTGTGACCTTGGTGATTTGAAAATGGATAAAAGAAGCATGAGTACTGAAACTCATGGTTTGTCTGGTGAGTTACTTGAAGGTGCAGTTAAATCTGTAATAGGTTCTGCGAGCATAGAAACAGGTATGAGACAGGAGACACCTTTTGCTGAAAGAGAGACTTCTGAGAAAGTTGAATCACGAGTTTGTCACTTCTCCTTCTTTGGTAAAGATTTGGTGGATTCCGTTAAGCTAGAAGCAAGTCAATGTGTTAATGCTGTAGACAGTGGCATGCATATCGCAAAAGATGGCAATCTCACTCAACCAAAGGGAAGTGCTATTCCATTGTTGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

578

Amino Acids

64.67

Weight (kDa)

4.8

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 221 - 424 9.9e-39 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017678)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04682
rosa_chinensis RchiOBHm_Chr3g0453161
rosa_laevigata RLG00000025532
rosa_multiflora Rmu_co8446773.1_g000001 Rmu_sc0007132.1_g000008
rosa_roxburghii Rroxscaffold_6G00430220
rosa_rugosa Rorug02G0646800
rosa_samantha Rh3AG049600 Rh3BG050800 Rh3CG051000 Rh3DG051600
rosa_wichuraiana Rw3G003810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 159, 1285, 1662
AciI CCGC 1 cut(s) 1217
AclWI GGATC 3 cut(s) 615, 1304, 1364
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 3 cut(s) 1123, 1192, 1447
AfiI CCNNNNNNNGG 3 cut(s) 1013, 1070, 1730
AflIII ACRYGT 1 cut(s) 597
AluBI AGCT 8 cut(s) 101, 189, 636, 662, 923, 1147, 1306, 1633
AluI AGCT 8 cut(s) 101, 189, 636, 662, 923, 1147, 1306, 1633
Alw21I GWGCWC 2 cut(s) 925, 1044
Alw26I GTCTC 5 cut(s) 188, 460, 1525, 1532, 1553
AlwI GGATC 3 cut(s) 615, 1304, 1364
AlwNI CAGNNNCTG 1 cut(s) 1325
AoxI GGCC 2 cut(s) 791, 982
ApeKI GCWGC 1 cut(s) 662
ArsI GACNNNNNNTTYG 4 cut(s) 805, 837, 1188, 1220
AseI ATTAAT 1 cut(s) 783
Asp700I GAANNNNTTC 1 cut(s) 1002
AspA2I CCTAGG 1 cut(s) 1007
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 1 cut(s) 982
AvrII CCTAGG 1 cut(s) 1007
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 1030
BanII GRGCYC 1 cut(s) 925
BarI GAAGNNNNNNTAC 2 cut(s) 1429, 1461
BauI CACGAG 1 cut(s) 1581
BbsI GAAGAC 1 cut(s) 813
Bbv12I GWGCWC 2 cut(s) 925, 1044
BbvI GCAGC 1 cut(s) 649
BccI CCATC 8 cut(s) 290, 380, 416, 506, 658, 734, 1334, 1682
BcgI CGANNNNNNTGC 2 cut(s) 1660, 1694
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 5 cut(s) 188, 460, 1525, 1532, 1553
BfaI CTAG 4 cut(s) 210, 1008, 1088, 1634
BfmI CTRYAG 1 cut(s) 1659
BfoI RGCGCY 1 cut(s) 731
BisI GCNGC 1 cut(s) 663
BlnI CCTAGG 1 cut(s) 1007
BlsI GCNGC 1 cut(s) 664
BmcAI AGTACT 1 cut(s) 1447
BmgT120I GGNCC 1 cut(s) 982
BmiI GGNNCC 2 cut(s) 870, 1176
BpiI GAAGAC 1 cut(s) 813
BpmI CTGGAG 2 cut(s) 96, 219
BpuEI CTTGAG 1 cut(s) 753
BsaBI GATNNNNATC 1 cut(s) 1692
BsaJI CCNNGG 3 cut(s) 1007, 1269, 1411
Bsc4I CCNNNNNNNGG 3 cut(s) 1013, 1070, 1730
Bse1I ACTGG 4 cut(s) 202, 890, 1056, 1204
Bse21I CCTNAGG 1 cut(s) 117
Bse8I GATNNNNATC 1 cut(s) 1692
BseDI CCNNGG 3 cut(s) 1007, 1269, 1411
BseGI GGATG 2 cut(s) 127, 517
BseJI GATNNNNATC 1 cut(s) 1692
BseLI CCNNNNNNNGG 3 cut(s) 1013, 1070, 1730
BseMII CTCAG 5 cut(s) 108, 477, 586, 1215, 1557
BseNI ACTGG 4 cut(s) 202, 890, 1056, 1204
BseRI GAGGAG 3 cut(s) 509, 628, 713
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 1 cut(s) 649
BsgI GTGCAG 2 cut(s) 1205, 1506
BshFI GGCC 2 cut(s) 793, 984
BsiHKAI GWGCWC 2 cut(s) 925, 1044
BslFI GGGAC 1 cut(s) 916
BslI CCNNNNNNNGG 3 cut(s) 1013, 1070, 1730
BsmAI GTCTC 5 cut(s) 188, 460, 1525, 1532, 1553
BsmFI GGGAC 1 cut(s) 916
BsmI GAATGC 2 cut(s) 508, 594
BsnI GGCC 2 cut(s) 793, 984
Bsp1286I GDGCHC 3 cut(s) 925, 1030, 1044
Bsp143I GATC 4 cut(s) 406, 607, 1309, 1369
BspACI CCGC 1 cut(s) 1217
BspANI GGCC 2 cut(s) 793, 984
BspCNI CTCAG 5 cut(s) 109, 478, 585, 1216, 1558
BspHI TCATGA 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 870, 1176
BspPI GGATC 3 cut(s) 615, 1304, 1364
BsrI ACTGG 4 cut(s) 202, 890, 1056, 1204
BssECI CCNNGG 3 cut(s) 1007, 1269, 1411
BssMI GATC 4 cut(s) 406, 607, 1309, 1369
BssSI CACGAG 1 cut(s) 1581
BssT1I CCWWGG 3 cut(s) 1007, 1269, 1411
Bst2BI CACGAG 1 cut(s) 1581
Bst4CI ACNGT 4 cut(s) 817, 1183, 1283, 1667
Bst6I CTCTTC 1 cut(s) 470
BstC8I GCNNGC 3 cut(s) 1221, 1513, 1673
BstDEI CTNAG 6 cut(s) 117, 486, 572, 756, 1224, 1566
BstEII GGTNACC 1 cut(s) 1054
BstENI CCTNNNNNAGG 1 cut(s) 1011
BstF5I GGATG 2 cut(s) 127, 517
BstH2I RGCGCY 1 cut(s) 731
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 4 cut(s) 409, 610, 1312, 1372
BstMAI GTCTC 5 cut(s) 188, 460, 1525, 1532, 1553
BstMBI GATC 4 cut(s) 406, 607, 1309, 1369
BstMWI GCNNNNNNNGC 2 cut(s) 29, 98
BstNSI RCATGY 1 cut(s) 1675
BstPI GGTNACC 1 cut(s) 1054
BstSFI CTRYAG 1 cut(s) 1659
BstSLI GKGCMC 1 cut(s) 1030
BstV1I GCAGC 1 cut(s) 649
BstV2I GAAGAC 1 cut(s) 813
BstXI CCANNNNNNTGG 2 cut(s) 880, 979
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 2 cut(s) 793, 984
BtsCI GGATG 2 cut(s) 127, 517
BtsI GCAGTG 2 cut(s) 513, 1193
BtsIMutI CAGTG 7 cut(s) 513, 580, 897, 1049, 1188, 1193, 1672
Cac8I GCNNGC 3 cut(s) 1221, 1513, 1673
CaiI CAGNNNCTG 1 cut(s) 1325
CciI TCATGA 1 cut(s) 409
CfoI GCGC 1 cut(s) 730
Cfr13I GGNCC 1 cut(s) 982
Csp6I GTAC 3 cut(s) 1122, 1191, 1446
CspCI CAANNNNNGTGG 2 cut(s) 1113, 1148
CviAII CATG 7 cut(s) 410, 593, 880, 1069, 1441, 1457, 1672
CviQI GTAC 3 cut(s) 1122, 1191, 1446
DdeI CTNAG 6 cut(s) 117, 486, 572, 756, 1224, 1566
DpnI GATC 4 cut(s) 408, 609, 1311, 1371
DpnII GATC 4 cut(s) 406, 607, 1309, 1369
DraI TTTAAA 1 cut(s) 910
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
Ecl136II GAGCTC 1 cut(s) 923
Eco130I CCWWGG 3 cut(s) 1007, 1269, 1411
Eco24I GRGCYC 1 cut(s) 925
Eco53kI GAGCTC 1 cut(s) 923
Eco57I CTGAAG 1 cut(s) 174
Eco81I CCTNAGG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 1054
EcoICRI GAGCTC 1 cut(s) 923
EcoNI CCTNNNNNAGG 1 cut(s) 1011
EcoO65I GGTNACC 1 cut(s) 1054
EcoT14I CCWWGG 3 cut(s) 1007, 1269, 1411
EcoT22I ATGCAT 3 cut(s) 594, 1156, 1677
EcoT38I GRGCYC 1 cut(s) 925
ErhI CCWWGG 3 cut(s) 1007, 1269, 1411
FaeI CATG 7 cut(s) 413, 596, 883, 1072, 1444, 1460, 1675
FalI AAGNNNNNCTT 4 cut(s) 960, 992, 1546, 1578
FaqI GGGAC 1 cut(s) 916
FatI CATG 7 cut(s) 409, 592, 879, 1068, 1440, 1456, 1671
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 3 cut(s) 159, 1285, 1662
Fnu4HI GCNGC 1 cut(s) 663
FokI GGATG 2 cut(s) 134, 524
FriOI GRGCYC 1 cut(s) 925
Fsp4HI GCNGC 1 cut(s) 663
FspBI CTAG 4 cut(s) 210, 1008, 1088, 1634
GlaI GCGC 1 cut(s) 729
GluI GCNGC 1 cut(s) 663
GsuI CTGGAG 2 cut(s) 96, 219
HaeII RGCGCY 1 cut(s) 731
HaeIII GGCC 2 cut(s) 793, 984
HhaI GCGC 1 cut(s) 730
Hin1II CATG 7 cut(s) 413, 596, 883, 1072, 1444, 1460, 1675
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HincII GTYRAC 1 cut(s) 1286
HindII GTYRAC 1 cut(s) 1286
HinfI GANTC 8 cut(s) 55, 108, 176, 538, 1236, 1276, 1577, 1622
Hpy166II GTNNAC 4 cut(s) 160, 1191, 1286, 1663
Hpy188I TCNGA 6 cut(s) 46, 154, 193, 697, 810, 1567
Hpy188III TCNNGA 6 cut(s) 59, 255, 410, 1139, 1394, 1581
Hpy8I GTNNAC 4 cut(s) 160, 1191, 1286, 1663
Hpy99I CGWCG 4 cut(s) 281, 347, 350, 353
HpyAV CCTTC 5 cut(s) 261, 484, 1069, 1475, 1609
HpyCH4III ACNGT 4 cut(s) 817, 1183, 1283, 1667
HpyCH4IV ACGT 1 cut(s) 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 98
HpyF3I CTNAG 6 cut(s) 117, 486, 572, 756, 1224, 1566
HpySE526I ACGT 1 cut(s) 599
Hsp92II CATG 7 cut(s) 413, 596, 883, 1072, 1444, 1460, 1675
HspAI GCGC 1 cut(s) 728
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 4 cut(s) 406, 607, 1309, 1369
LmnI GCTCC 8 cut(s) 77, 194, 451, 641, 928, 1039, 1180, 1303
Lsp1109I GCAGC 1 cut(s) 649
MaeI CTAG 4 cut(s) 210, 1008, 1088, 1634
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 5 cut(s) 1054, 1277, 1406, 1473, 1589
MalI GATC 4 cut(s) 408, 609, 1311, 1371
MboI GATC 4 cut(s) 406, 607, 1309, 1369
MboII GAAGA 6 cut(s) 64, 377, 487, 511, 715, 813
MfeI CAATTG 1 cut(s) 470
MhlI GDGCHC 3 cut(s) 925, 1030, 1044
MluCI AATT 9 cut(s) 137, 168, 227, 470, 566, 613, 668, 1002, 1363
MlyI GAGTC 3 cut(s) 102, 547, 1285
MmeI TCCRAC 1 cut(s) 1202
Mph1103I ATGCAT 3 cut(s) 594, 1156, 1677
MroXI GAANNNNTTC 1 cut(s) 1002
MslI CAYNNNNRTG 1 cut(s) 414
MspA1I CMGCKG 1 cut(s) 662
MunI CAATTG 1 cut(s) 470
Mva1269I GAATGC 2 cut(s) 508, 594
MwoI GCNNNNNNNGC 2 cut(s) 29, 98
NdeII GATC 4 cut(s) 406, 607, 1309, 1369
NlaIII CATG 7 cut(s) 413, 596, 883, 1072, 1444, 1460, 1675
NlaIV GGNNCC 2 cut(s) 870, 1176
NmuCI GTSAC 4 cut(s) 1054, 1277, 1406, 1589
NsiI ATGCAT 3 cut(s) 594, 1156, 1677
NspI RCATGY 1 cut(s) 1675
PaeI GCATGC 1 cut(s) 1675
PagI TCATGA 1 cut(s) 409
PcsI WCGNNNNNNNCGW 3 cut(s) 342, 348, 354
PctI GAATGC 2 cut(s) 508, 594
PdmI GAANNNNTTC 1 cut(s) 1002
PfeI GAWTC 5 cut(s) 55, 176, 1236, 1577, 1622
PflFI GACNNNGTC 1 cut(s) 817
PkrI GCNGC 1 cut(s) 664
PleI GAGTC 3 cut(s) 102, 546, 1284
PpsI GAGTC 3 cut(s) 102, 546, 1284
PshBI ATTAAT 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 925
PspEI GGTNACC 1 cut(s) 1054
PspN4I GGNNCC 2 cut(s) 870, 1176
PspPI GGNCC 1 cut(s) 982
PstNI CAGNNNCTG 1 cut(s) 1325
PsyI GACNNNGTC 1 cut(s) 817
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 3 cut(s) 1123, 1192, 1447
RsaNI GTAC 3 cut(s) 1122, 1191, 1446
RseI CAYNNNNRTG 1 cut(s) 414
SacI GAGCTC 1 cut(s) 925
SalI GTCGAC 1 cut(s) 1284
SatI GCNGC 1 cut(s) 663
Sau3AI GATC 4 cut(s) 406, 607, 1309, 1369
Sau96I GGNCC 1 cut(s) 982
ScaI AGTACT 1 cut(s) 1447
SchI GAGTC 3 cut(s) 102, 547, 1285
SduI GDGCHC 3 cut(s) 925, 1030, 1044
SfcI CTRYAG 1 cut(s) 1659
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 768
SmoI CTYRAG 1 cut(s) 768
SphI GCATGC 1 cut(s) 1675
Sse9I AATT 9 cut(s) 137, 168, 227, 470, 566, 613, 668, 1002, 1363
SsiI CCGC 1 cut(s) 1217
SspMI CTAG 4 cut(s) 210, 1008, 1088, 1634
SstI GAGCTC 1 cut(s) 925
StyI CCWWGG 3 cut(s) 1007, 1269, 1411
TaaI ACNGT 4 cut(s) 817, 1183, 1283, 1667
TaiI ACGT 1 cut(s) 602
TaqI TCGA 2 cut(s) 234, 1285
TasI AATT 9 cut(s) 137, 168, 227, 470, 566, 613, 668, 1002, 1363
TatI WGTACW 2 cut(s) 1121, 1445
TfiI GAWTC 5 cut(s) 55, 176, 1236, 1577, 1622
TscAI CASTG 7 cut(s) 513, 580, 897, 1056, 1188, 1193, 1672
TseFI GTSAC 4 cut(s) 1054, 1277, 1406, 1589
TseI GCWGC 1 cut(s) 662
Tsp45I GTSAC 4 cut(s) 1054, 1277, 1406, 1589
TspDTI ATGAA 3 cut(s) 65, 378, 939
TspGWI ACGGA 1 cut(s) 1615
TspRI CASTG 7 cut(s) 513, 580, 897, 1056, 1188, 1193, 1672
Tth111I GACNNNGTC 1 cut(s) 817
VspI ATTAAT 1 cut(s) 783
XagI CCTNNNNNAGG 1 cut(s) 1011
XceI RCATGY 1 cut(s) 1675
XmaJI CCTAGG 1 cut(s) 1007
XmiI GTMKAC 3 cut(s) 159, 1285, 1662
XmnI GAANNNNTTC 1 cut(s) 1002
XspI CTAG 4 cut(s) 210, 1008, 1088, 1634
ZrmI AGTACT 1 cut(s) 1447
Zsp2I ATGCAT 3 cut(s) 594, 1156, 1677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.