RLG00000025532

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017678
Physical Location & Seq
Reverse (-)
46513404 .. 46515131
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025532

Sequence Viewer

Length: 1728 bp
ATGGAGAGGAACAAAGGGGAGGCAGTCAGGGCAATGCAACTTTCAGAAATGAAGATTCAAGAGAATGATTTTGCTGGAGCTAGGGAAATGGCACGAATAGCTCACCGACTCTTCCCTGAGGATGAGAACATTTCTAAATTACTTGCACTTTCTGAAGTCTACTATTCAATTTTTGAATCTGGTGAAAGCTCCGAGACCGGAGATACTGCTAGAGCTGATTTTGAGCAATTTATCGAAAAGGTCAGTAGTCCTTCTGGAAATCTAAATGATGATGACGACGATGATGATAGTGATGATGGTGATGATGATAGTGATGATGATTATGATTATGATGATGATGACGACGATGAAGATGAAGATGATGATTATGATTCTGATGGCAACAATGAGTATGATGGTCGTGATGATGATGATGGTGATGACGATTTTGTGATTGCTCCCAAAAGGTTGAGGGAGAGACAATTGAAGAGTTATACTGAGAAGGAGGAGAAGAATGCAGTGGATGGTGGAGTATCTAAAAGTGTTGTGAGTCGTCCAAAAAGGTTGAGGCACAGCCAATTCCTTAGTGTTATTAACAGAATGCATGACACGTTTAAGGATCATAATTCTGTCAGATCCATTAAAAGCTCCTCTACCCCTCCATCGTCATCAGCTGCTCAATTCTATGACTTCAATGCACAAAAGTCTGAGGAGAAGATTAGGGTTGGTCAAATATGGGCACTGTATAGTGATGGGAATGGAATGTCTAAGACCTTTGCTCAAGTTAAGAGGATTAATGTCAGGCCAAACAAAAGAGTTCAGAGGACTCTGTCTTCGGGGCATTTTTATTTGCGAATGGGATTGCTTGAACCTTTCTCAGAACCCAAAAACATGGTTGAGCCGGTGTGTTGTGGGACATTTAAAGTCAAACGAGCTCCACCTACACTGTTCTCCCTCACCTCATTATCTCATTGTGTAAAAGCCAGACTTTTGGGCCAGAATATATTTGAAATAATTCCTAGGAGAGGTGAAGTTTGGGCACTATACAAGGAGCACAATCCACTGGTGACCTATCCTAACATGGGGAAGGGTGAATGTCTAGTGGTGGAAGTGCTGGAAGTTCAGCACCAGAGTACAAGGATTGTAGTTCTGGAACAGCTTAATGGATTTAAGTCAGTTTTCAAGGCTCCAACAGTGCAGAGTACCAAAACTGGTGTCATTGATATACCGCAGGCTGAGTTGGGCAGATTCTCTCACCAGATTTCTGCTTTTAAGCATACCAAGGTGAGTCACAGTCGACTTGCAGGGTGTTGGGAGCTTGATCCATTATCAGTTTCTGGTTATGAGAAAGATGGTTTTCCCAAATGTTTCTTTAATTGTGATCCTGCTTCTCTGCTGAACAACCCTGACTGCTCCCATGACCTTGGTGATTTGAAAATGGATAAAAGAAGCATGAATACTGAAACTCATGGTTTGTCTGGTGAGTTACTTGAAGGTGCAGCTAAATCTGTAATAGGTTCTGCGAGCATAGATACAGGTATGAAACAGGAGACACCTTTTGCTGAAAGAGAAACTTCTGAGAAAGTTGAATCACGAGTTTGTCACTTCTCCTTCTTTGGTAAAGATTTGGTGGATTCCGTTAAGCTAGAAGCAAGTCAATGTGTTAATGCTGTAGCCAATGGCATGCATATCGCAAAAGATGGCAATCTCACTCAACCAAAGGGAAGTGCCATTCCATTGTTGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

576

Amino Acids

64.2

Weight (kDa)

4.94

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 218 - 421 6.2e-40 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017678)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04682
rosa_chinensis RchiOBHm_Chr3g0453161
rosa_laevigata RLG00000025532
rosa_multiflora Rmu_co8446773.1_g000001 Rmu_sc0007132.1_g000008
rosa_roxburghii Rroxscaffold_6G00430220
rosa_rugosa Rorug02G0646800
rosa_samantha Rh3AG049600 Rh3BG050800 Rh3CG051000 Rh3DG051600
rosa_wichuraiana Rw3G003810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 159, 1276
AciI CCGC 1 cut(s) 1208
AclWI GGATC 4 cut(s) 606, 609, 1295, 1355
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 2 cut(s) 1114, 1183
AfiI CCNNNNNNNGG 3 cut(s) 1004, 1061, 1721
AflIII ACRYGT 1 cut(s) 588
Alw21I GWGCWC 2 cut(s) 916, 1035
Alw26I GTCTC 3 cut(s) 188, 451, 1523
AlwI GGATC 4 cut(s) 606, 609, 1295, 1355
AlwNI CAGNNNCTG 1 cut(s) 1316
AoxI GGCC 2 cut(s) 782, 973
ApeKI GCWGC 2 cut(s) 653, 1478
ArsI GACNNNNNNTTYG 4 cut(s) 796, 828, 1179, 1211
AseI ATTAAT 1 cut(s) 774
Asp700I GAANNNNTTC 1 cut(s) 993
AspA2I CCTAGG 1 cut(s) 998
AspS9I GGNCC 1 cut(s) 973
AvrII CCTAGG 1 cut(s) 998
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 2 cut(s) 721, 1021
BanII GRGCYC 1 cut(s) 916
BarI GAAGNNNNNNTAC 2 cut(s) 1420, 1452
BauI CACGAG 1 cut(s) 1572
BbsI GAAGAC 1 cut(s) 804
Bbv12I GWGCWC 2 cut(s) 916, 1035
BbvI GCAGC 2 cut(s) 640, 1490
BccI CCATC 9 cut(s) 290, 371, 389, 407, 497, 649, 725, 1325, 1673
BcgI CGANNNNNNTGC 2 cut(s) 1651, 1685
BcoDI GTCTC 3 cut(s) 188, 451, 1523
BfaI CTAG 5 cut(s) 81, 210, 999, 1079, 1625
BfmI CTRYAG 1 cut(s) 1650
BisI GCNGC 2 cut(s) 654, 1479
BlnI CCTAGG 1 cut(s) 998
BlsI GCNGC 2 cut(s) 655, 1480
BmgT120I GGNCC 1 cut(s) 973
BmiI GGNNCC 1 cut(s) 1167
BpiI GAAGAC 1 cut(s) 804
BpmI CTGGAG 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 744
BsaBI GATNNNNATC 1 cut(s) 1683
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 998, 1260, 1402
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 3 cut(s) 1004, 1061, 1721
Bse118I RCCGGY 1 cut(s) 880
Bse1I ACTGG 2 cut(s) 1047, 1195
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 39
Bse8I GATNNNNATC 1 cut(s) 1683
BseDI CCNNGG 3 cut(s) 998, 1260, 1402
BseGI GGATG 2 cut(s) 127, 508
BseJI GATNNNNATC 1 cut(s) 1683
BseLI CCNNNNNNNGG 3 cut(s) 1004, 1061, 1721
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 6 cut(s) 108, 468, 678, 870, 1206, 1548
BseNI ACTGG 2 cut(s) 1047, 1195
BseRI GAGGAG 3 cut(s) 500, 619, 704
BseSI GKGCMC 2 cut(s) 721, 1021
BseXI GCAGC 2 cut(s) 640, 1490
BsgI GTGCAG 2 cut(s) 1196, 1497
BshFI GGCC 2 cut(s) 784, 975
BsiHKAI GWGCWC 2 cut(s) 916, 1035
BsiSI CCGG 2 cut(s) 198, 881
BslFI GGGAC 1 cut(s) 907
BslI CCNNNNNNNGG 3 cut(s) 1004, 1061, 1721
BsmAI GTCTC 3 cut(s) 188, 451, 1523
BsmFI GGGAC 1 cut(s) 907
BsmI GAATGC 2 cut(s) 499, 585
BsnI GGCC 2 cut(s) 784, 975
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 4 cut(s) 721, 916, 1021, 1035
Bsp143I GATC 4 cut(s) 598, 614, 1300, 1360
BspACI CCGC 1 cut(s) 1208
BspANI GGCC 2 cut(s) 784, 975
BspCNI CTCAG 6 cut(s) 109, 469, 679, 869, 1207, 1549
BspLI GGNNCC 1 cut(s) 1167
BspPI GGATC 4 cut(s) 606, 609, 1295, 1355
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 880
BsrI ACTGG 2 cut(s) 1047, 1195
BssAI RCCGGY 1 cut(s) 880
BssECI CCNNGG 3 cut(s) 998, 1260, 1402
BssMI GATC 4 cut(s) 598, 614, 1300, 1360
BssSI CACGAG 1 cut(s) 1572
BssT1I CCWWGG 3 cut(s) 998, 1260, 1402
Bst2BI CACGAG 1 cut(s) 1572
Bst4CI ACNGT 4 cut(s) 723, 927, 1174, 1274
Bst6I CTCTTC 2 cut(s) 116, 461
BstC8I GCNNGC 3 cut(s) 1212, 1504, 1664
BstDEI CTNAG 8 cut(s) 117, 477, 563, 687, 747, 856, 1215, 1557
BstEII GGTNACC 1 cut(s) 1045
BstENI CCTNNNNNAGG 1 cut(s) 1002
BstF5I GGATG 2 cut(s) 127, 508
BstKTI GATC 4 cut(s) 601, 617, 1303, 1363
BstMAI GTCTC 3 cut(s) 188, 451, 1523
BstMBI GATC 4 cut(s) 598, 614, 1300, 1360
BstMWI GCNNNNNNNGC 2 cut(s) 29, 98
BstNSI RCATGY 1 cut(s) 1666
BstPI GGTNACC 1 cut(s) 1045
BstSFI CTRYAG 1 cut(s) 1650
BstSLI GKGCMC 2 cut(s) 721, 1021
BstV1I GCAGC 2 cut(s) 640, 1490
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 1 cut(s) 614
BstXI CCANNNNNNTGG 3 cut(s) 871, 970, 1403
BstYI RGATCY 1 cut(s) 614
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 2 cut(s) 784, 975
BtsCI GGATG 2 cut(s) 127, 508
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 5 cut(s) 504, 719, 923, 1040, 1179
Cac8I GCNNGC 3 cut(s) 1212, 1504, 1664
CaiI CAGNNNCTG 1 cut(s) 1316
Cfr10I RCCGGY 1 cut(s) 880
Cfr13I GGNCC 1 cut(s) 973
Csp6I GTAC 2 cut(s) 1113, 1182
CviAII CATG 7 cut(s) 584, 871, 1060, 1397, 1432, 1448, 1663
CviQI GTAC 2 cut(s) 1113, 1182
DdeI CTNAG 8 cut(s) 117, 477, 563, 687, 747, 856, 1215, 1557
DpnI GATC 4 cut(s) 600, 616, 1302, 1362
DpnII GATC 4 cut(s) 598, 614, 1300, 1360
DraI TTTAAA 1 cut(s) 901
Eam1104I CTCTTC 2 cut(s) 116, 461
EarI CTCTTC 2 cut(s) 116, 461
Ecl136II GAGCTC 1 cut(s) 914
Eco130I CCWWGG 3 cut(s) 998, 1260, 1402
Eco24I GRGCYC 1 cut(s) 916
Eco31I GGTCTC 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 914
Eco57I CTGAAG 1 cut(s) 174
Eco81I CCTNAGG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 1045
EcoICRI GAGCTC 1 cut(s) 914
EcoNI CCTNNNNNAGG 1 cut(s) 1002
EcoO65I GGTNACC 1 cut(s) 1045
EcoT14I CCWWGG 3 cut(s) 998, 1260, 1402
EcoT22I ATGCAT 2 cut(s) 585, 1668
EcoT38I GRGCYC 1 cut(s) 916
ErhI CCWWGG 3 cut(s) 998, 1260, 1402
FaeI CATG 7 cut(s) 587, 874, 1063, 1400, 1435, 1451, 1666
FalI AAGNNNNNCTT 4 cut(s) 951, 983, 1537, 1569
FaqI GGGAC 1 cut(s) 907
FatI CATG 7 cut(s) 583, 870, 1059, 1396, 1431, 1447, 1662
FblI GTMKAC 2 cut(s) 159, 1276
Fnu4HI GCNGC 2 cut(s) 654, 1479
FokI GGATG 2 cut(s) 134, 515
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 2 cut(s) 654, 1479
FspBI CTAG 5 cut(s) 81, 210, 999, 1079, 1625
GluI GCNGC 2 cut(s) 654, 1479
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 784, 975
HapII CCGG 2 cut(s) 198, 881
Hin1II CATG 7 cut(s) 587, 874, 1063, 1400, 1435, 1451, 1666
HincII GTYRAC 1 cut(s) 1277
HindII GTYRAC 1 cut(s) 1277
HpaII CCGG 2 cut(s) 198, 881
Hpy166II GTNNAC 2 cut(s) 160, 1277
Hpy188I TCNGA 9 cut(s) 46, 154, 193, 376, 614, 688, 801, 859, 1558
Hpy188III TCNNGA 5 cut(s) 59, 255, 401, 1130, 1572
Hpy8I GTNNAC 2 cut(s) 160, 1277
Hpy99I CGWCG 2 cut(s) 281, 347
HpyAV CCTTC 5 cut(s) 261, 475, 1060, 1466, 1600
HpyCH4III ACNGT 4 cut(s) 723, 927, 1174, 1274
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 9 cut(s) 37, 146, 497, 583, 677, 1177, 1283, 1478, 1666
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 98
HpyF3I CTNAG 8 cut(s) 117, 477, 563, 687, 747, 856, 1215, 1557
HpySE526I ACGT 1 cut(s) 590
Hsp92II CATG 7 cut(s) 587, 874, 1063, 1400, 1435, 1451, 1666
Kzo9I GATC 4 cut(s) 598, 614, 1300, 1360
LmnI GCTCC 9 cut(s) 77, 194, 442, 632, 919, 1030, 1171, 1294, 1397
Lsp1109I GCAGC 2 cut(s) 640, 1490
MaeI CTAG 5 cut(s) 81, 210, 999, 1079, 1625
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 4 cut(s) 1045, 1268, 1464, 1580
MalI GATC 4 cut(s) 600, 616, 1302, 1362
MboI GATC 4 cut(s) 598, 614, 1300, 1360
MboII GAAGA 8 cut(s) 64, 103, 362, 368, 478, 502, 706, 804
MfeI CAATTG 1 cut(s) 461
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 4 cut(s) 721, 916, 1021, 1035
MluCI AATT 9 cut(s) 137, 168, 227, 461, 557, 604, 659, 993, 1354
MlyI GAGTC 4 cut(s) 102, 538, 799, 1276
MmeI TCCRAC 1 cut(s) 1193
Mph1103I ATGCAT 2 cut(s) 585, 1668
MroXI GAANNNNTTC 1 cut(s) 993
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 2 cut(s) 198, 881
MunI CAATTG 1 cut(s) 461
Mva1269I GAATGC 2 cut(s) 499, 585
MwoI GCNNNNNNNGC 2 cut(s) 29, 98
NdeII GATC 4 cut(s) 598, 614, 1300, 1360
NlaIII CATG 7 cut(s) 587, 874, 1063, 1400, 1435, 1451, 1666
NlaIV GGNNCC 1 cut(s) 1167
NmuCI GTSAC 3 cut(s) 1045, 1268, 1580
NsiI ATGCAT 2 cut(s) 585, 1668
NspI RCATGY 1 cut(s) 1666
PaeI GCATGC 1 cut(s) 1666
PctI GAATGC 2 cut(s) 499, 585
PdmI GAANNNNTTC 1 cut(s) 993
PfeI GAWTC 6 cut(s) 55, 176, 371, 1227, 1568, 1613
PflFI GACNNNGTC 1 cut(s) 808
PkrI GCNGC 2 cut(s) 655, 1480
PleI GAGTC 4 cut(s) 102, 537, 799, 1275
PpsI GAGTC 4 cut(s) 102, 537, 799, 1275
PshBI ATTAAT 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 916
PspEI GGTNACC 1 cut(s) 1045
PspN4I GGNNCC 1 cut(s) 1167
PspPI GGNCC 1 cut(s) 973
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 1 cut(s) 614
PsyI GACNNNGTC 1 cut(s) 808
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 2 cut(s) 1114, 1183
RsaNI GTAC 2 cut(s) 1113, 1182
SacI GAGCTC 1 cut(s) 916
SalI GTCGAC 1 cut(s) 1275
SatI GCNGC 2 cut(s) 654, 1479
Sau3AI GATC 4 cut(s) 598, 614, 1300, 1360
Sau96I GGNCC 1 cut(s) 973
SchI GAGTC 4 cut(s) 102, 538, 799, 1276
SduI GDGCHC 4 cut(s) 721, 916, 1021, 1035
SfcI CTRYAG 1 cut(s) 1650
SmlI CTYRAG 1 cut(s) 759
SmoI CTYRAG 1 cut(s) 759
SphI GCATGC 1 cut(s) 1666
Sse9I AATT 9 cut(s) 137, 168, 227, 461, 557, 604, 659, 993, 1354
SsiI CCGC 1 cut(s) 1208
SspMI CTAG 5 cut(s) 81, 210, 999, 1079, 1625
SstI GAGCTC 1 cut(s) 916
StyI CCWWGG 3 cut(s) 998, 1260, 1402
TaaI ACNGT 4 cut(s) 723, 927, 1174, 1274
TaiI ACGT 1 cut(s) 593
TaqI TCGA 2 cut(s) 234, 1276
TasI AATT 9 cut(s) 137, 168, 227, 461, 557, 604, 659, 993, 1354
TatI WGTACW 1 cut(s) 1112
TfiI GAWTC 6 cut(s) 55, 176, 371, 1227, 1568, 1613
TscAI CASTG 5 cut(s) 504, 726, 930, 1047, 1179
TseFI GTSAC 3 cut(s) 1045, 1268, 1580
TseI GCWGC 2 cut(s) 653, 1478
Tsp45I GTSAC 3 cut(s) 1045, 1268, 1580
TspDTI ATGAA 5 cut(s) 65, 363, 369, 1448, 1535
TspGWI ACGGA 1 cut(s) 1606
TspRI CASTG 5 cut(s) 504, 726, 930, 1047, 1179
Tth111I GACNNNGTC 1 cut(s) 808
VspI ATTAAT 1 cut(s) 774
XagI CCTNNNNNAGG 1 cut(s) 1002
XceI RCATGY 1 cut(s) 1666
XcmI CCANNNNNNNNNTGG 1 cut(s) 1717
XmaJI CCTAGG 1 cut(s) 998
XmiI GTMKAC 2 cut(s) 159, 1276
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 5 cut(s) 81, 210, 999, 1079, 1625
Zsp2I ATGCAT 2 cut(s) 585, 1668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.