Rw3G003810

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017678
Physical Location & Seq
Forward (+)
3282805 .. 3284541
1737 bp
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UTR
Exon/CDS
Intron
Rw3G003810.1

Sequence Viewer

Length: 1485 bp
ATGGAGAGGAACAAAGGGGAGGCAGTCAGGGCTATGCAACTTTCAGAAATGAAGATTCAAGAGAATGATTTTGCTGGAGCAAGGGAAATGGCACGAATAGCTCACCGACTCTACCCTGAGGATGAGAACATTTCTAAATTACTTGCACTCTCTGAAGTCTACTATTCAATTTTTGAATCTGGTGAAAGCTCCGAGACTGGAGATACTGCTAGAGATGATTTTGAGCAATTTATCGAAAAGGAGGAGAAGAATGCAGTGGATGGTGGAGTATCTAAAAGTGTTGTGAGTCGTCCAAAAAGGTTGAGGCACAGCCAATTCCTCAGTGTTATTAACAGAATGCATGACACGTTTAAGGATCATAATTCTGGCAGTTCCATTAAAAGCTCCTCTACCCCTCCATCGTCATCAGCTGCTCAATTCTATGACTTCAATGCACAAAAGTCTGAGGAGAAGATTAGGGTTGGTCAAATATGGGCGCTGTATGGTGATGGGAATGGAATGTCTAAGACCTTTGCTCAAGTTAAGAGGATTAATGTCAGGCCAAACAAAAGAGTTCAGAGGACTGTGTCTTCGGGGCATTTTTATTTGCGAATGGGATTGCTTGAACCTTTCTTGGAACCCAAAAACATGGTTGAGCCAGTGTGTTGTGGGACATTTAAAGTCAAACGAGCTCCACCTACACTTTTCTCCCTCACTTCATTATCTCATCGTGTAAAAGCCAGACTTTTGGGCCAGAATATATTTGAAATAATTCCTAGGAGAGGTGAAGTGTGGGCACTATACAAGGAGCACAATCCACTGGTGACCTATCCTAACATGGGGAAGGGTGAATGTCTAGTGGTGGAAGTGCTAGAAGTTCAGCACCAGAGTACAAAGATTGTGGTTCTGGAACAGCTTAATGCATTTAAGTCAGTTTTCAAGGCTCCAACAGTGCAGAGTACCAAAACTGGTGTCATTGATATACCGCAGGCTGAGTTGGGTAGATTCTCTCACCAGATTTCTGCTTTTAAGCATACCAAGGTGAGTCACAGTCGACTTGCAGGGTGTTGGGAGCTTGATCCATTATCAGTTTCTGGTTATGAGAAAGATGGTGTTCCCAAATGTTTCTTTAATTGTGATCCTGCTTCTCTGCTTAACAATCCTGACTACTCCTGTGACCTTGGTGATTTGAAAATGGATAAAAGAAGCATGAGTACTGAAACTCATGGTTTGTCTGGTGAGTTACTTGAAGGTGCAGTTAAATCTGTAATAGGTTCTGCGAGCATAGAAACAGGTACGAGAAAGGAGACACCTTTTGCTGAAAGAGAGACTTCTGAGAAAGTTGAATCACGAGTTTGTCACTTCTCCTTCTTTGGTAAAGATTTGGTGGATTCCGTTAAGCTAGAAGCAAGTCAATGTGTTAATGCTGTAGACAGTGGCATGCATATCGCAAAAGATGGCAATCTCACTCAACCAAAGGGAAGTGCTATTCCATTGTTGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

494

Amino Acids

54.92

Weight (kDa)

7.99

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 137 - 340 2.7e-39 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017678)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04682
rosa_chinensis RchiOBHm_Chr3g0453161
rosa_laevigata RLG00000025532
rosa_multiflora Rmu_co8446773.1_g000001 Rmu_sc0007132.1_g000008
rosa_roxburghii Rroxscaffold_6G00430220
rosa_rugosa Rorug02G0646800
rosa_samantha Rh3AG049600 Rh3BG050800 Rh3CG051000 Rh3DG051600
rosa_wichuraiana Rw3G003810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 159, 1033, 1410
AciI CCGC 1 cut(s) 965
AclWI GGATC 3 cut(s) 363, 1052, 1112
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 4 cut(s) 871, 940, 1195, 1276
AfiI CCNNNNNNNGG 3 cut(s) 761, 818, 1478
AflIII ACRYGT 1 cut(s) 345
AluBI AGCT 8 cut(s) 101, 189, 384, 410, 671, 895, 1054, 1381
AluI AGCT 8 cut(s) 101, 189, 384, 410, 671, 895, 1054, 1381
Alw21I GWGCWC 2 cut(s) 673, 792
Alw26I GTCTC 3 cut(s) 188, 1280, 1301
AlwI GGATC 3 cut(s) 363, 1052, 1112
AlwNI CAGNNNCTG 1 cut(s) 1073
AoxI GGCC 2 cut(s) 539, 730
ApeKI GCWGC 1 cut(s) 410
ArsI GACNNNNNNTTYG 4 cut(s) 553, 585, 936, 968
AseI ATTAAT 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 750
AspA2I CCTAGG 1 cut(s) 755
AspLEI GCGC 1 cut(s) 478
AspS9I GGNCC 1 cut(s) 730
AvrII CCTAGG 1 cut(s) 755
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 778
BanII GRGCYC 1 cut(s) 673
BarI GAAGNNNNNNTAC 2 cut(s) 1177, 1209
BauI CACGAG 1 cut(s) 1329
BbsI GAAGAC 1 cut(s) 561
Bbv12I GWGCWC 2 cut(s) 673, 792
BbvI GCAGC 1 cut(s) 397
BccI CCATC 5 cut(s) 254, 406, 482, 1082, 1430
BcgI CGANNNNNNTGC 2 cut(s) 1408, 1442
BcoDI GTCTC 3 cut(s) 188, 1280, 1301
BfaI CTAG 5 cut(s) 210, 756, 836, 851, 1382
BfmI CTRYAG 1 cut(s) 1407
BfoI RGCGCY 1 cut(s) 479
BisI GCNGC 1 cut(s) 411
BlnI CCTAGG 1 cut(s) 755
BlsI GCNGC 1 cut(s) 412
BmcAI AGTACT 1 cut(s) 1195
BmgT120I GGNCC 1 cut(s) 730
BmiI GGNNCC 2 cut(s) 618, 924
BpiI GAAGAC 1 cut(s) 561
BpmI CTGGAG 2 cut(s) 96, 219
BpuEI CTTGAG 1 cut(s) 501
BsaBI GATNNNNATC 1 cut(s) 1440
BsaJI CCNNGG 3 cut(s) 755, 1017, 1159
Bsc4I CCNNNNNNNGG 3 cut(s) 761, 818, 1478
Bse1I ACTGG 4 cut(s) 202, 638, 804, 952
Bse21I CCTNAGG 1 cut(s) 117
Bse8I GATNNNNATC 1 cut(s) 1440
BseDI CCNNGG 3 cut(s) 755, 1017, 1159
BseGI GGATG 2 cut(s) 127, 265
BseJI GATNNNNATC 1 cut(s) 1440
BseLI CCNNNNNNNGG 3 cut(s) 761, 818, 1478
BseMII CTCAG 5 cut(s) 108, 334, 435, 963, 1305
BseNI ACTGG 4 cut(s) 202, 638, 804, 952
BseRI GAGGAG 3 cut(s) 257, 376, 461
BseSI GKGCMC 1 cut(s) 778
BseXI GCAGC 1 cut(s) 397
BsgI GTGCAG 2 cut(s) 953, 1254
BshFI GGCC 2 cut(s) 541, 732
BsiHKAI GWGCWC 2 cut(s) 673, 792
BslFI GGGAC 1 cut(s) 664
BslI CCNNNNNNNGG 3 cut(s) 761, 818, 1478
BsmAI GTCTC 3 cut(s) 188, 1280, 1301
BsmFI GGGAC 1 cut(s) 664
BsmI GAATGC 2 cut(s) 256, 342
BsnI GGCC 2 cut(s) 541, 732
Bsp1286I GDGCHC 3 cut(s) 673, 778, 792
Bsp143I GATC 3 cut(s) 355, 1057, 1117
BspACI CCGC 1 cut(s) 965
BspANI GGCC 2 cut(s) 541, 732
BspCNI CTCAG 5 cut(s) 109, 333, 436, 964, 1306
BspLI GGNNCC 2 cut(s) 618, 924
BspPI GGATC 3 cut(s) 363, 1052, 1112
BsrI ACTGG 4 cut(s) 202, 638, 804, 952
BssECI CCNNGG 3 cut(s) 755, 1017, 1159
BssMI GATC 3 cut(s) 355, 1057, 1117
BssSI CACGAG 1 cut(s) 1329
BssT1I CCWWGG 3 cut(s) 755, 1017, 1159
Bst2BI CACGAG 1 cut(s) 1329
Bst4CI ACNGT 4 cut(s) 565, 931, 1031, 1415
BstC8I GCNNGC 3 cut(s) 969, 1261, 1421
BstDEI CTNAG 6 cut(s) 117, 320, 444, 504, 972, 1314
BstEII GGTNACC 1 cut(s) 802
BstENI CCTNNNNNAGG 1 cut(s) 759
BstF5I GGATG 2 cut(s) 127, 265
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 3 cut(s) 358, 1060, 1120
BstMAI GTCTC 3 cut(s) 188, 1280, 1301
BstMBI GATC 3 cut(s) 355, 1057, 1117
BstMWI GCNNNNNNNGC 2 cut(s) 29, 98
BstNSI RCATGY 1 cut(s) 1423
BstPI GGTNACC 1 cut(s) 802
BstSFI CTRYAG 1 cut(s) 1407
BstSLI GKGCMC 1 cut(s) 778
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 1 cut(s) 561
BstXI CCANNNNNNTGG 2 cut(s) 628, 727
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 2 cut(s) 541, 732
BtsCI GGATG 2 cut(s) 127, 265
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 6 cut(s) 261, 328, 645, 797, 936, 1420
Cac8I GCNNGC 3 cut(s) 969, 1261, 1421
CaiI CAGNNNCTG 1 cut(s) 1073
CfoI GCGC 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 730
Csp6I GTAC 4 cut(s) 870, 939, 1194, 1275
CspCI CAANNNNNGTGG 2 cut(s) 861, 896
CviAII CATG 6 cut(s) 341, 628, 817, 1189, 1205, 1420
CviQI GTAC 4 cut(s) 870, 939, 1194, 1275
DdeI CTNAG 6 cut(s) 117, 320, 444, 504, 972, 1314
DpnI GATC 3 cut(s) 357, 1059, 1119
DpnII GATC 3 cut(s) 355, 1057, 1117
DraI TTTAAA 1 cut(s) 658
Ecl136II GAGCTC 1 cut(s) 671
Eco130I CCWWGG 3 cut(s) 755, 1017, 1159
Eco24I GRGCYC 1 cut(s) 673
Eco53kI GAGCTC 1 cut(s) 671
Eco57I CTGAAG 1 cut(s) 174
Eco81I CCTNAGG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 802
EcoICRI GAGCTC 1 cut(s) 671
EcoNI CCTNNNNNAGG 1 cut(s) 759
EcoO65I GGTNACC 1 cut(s) 802
EcoT14I CCWWGG 3 cut(s) 755, 1017, 1159
EcoT22I ATGCAT 3 cut(s) 342, 904, 1425
EcoT38I GRGCYC 1 cut(s) 673
ErhI CCWWGG 3 cut(s) 755, 1017, 1159
FaeI CATG 6 cut(s) 344, 631, 820, 1192, 1208, 1423
FalI AAGNNNNNCTT 4 cut(s) 708, 740, 1294, 1326
FaqI GGGAC 1 cut(s) 664
FatI CATG 6 cut(s) 340, 627, 816, 1188, 1204, 1419
FblI GTMKAC 3 cut(s) 159, 1033, 1410
Fnu4HI GCNGC 1 cut(s) 411
FokI GGATG 2 cut(s) 134, 272
FriOI GRGCYC 1 cut(s) 673
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 5 cut(s) 210, 756, 836, 851, 1382
GlaI GCGC 1 cut(s) 477
GluI GCNGC 1 cut(s) 411
GsuI CTGGAG 2 cut(s) 96, 219
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 2 cut(s) 541, 732
HhaI GCGC 1 cut(s) 478
Hin1II CATG 6 cut(s) 344, 631, 820, 1192, 1208, 1423
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HincII GTYRAC 1 cut(s) 1034
HindII GTYRAC 1 cut(s) 1034
HinfI GANTC 8 cut(s) 55, 108, 176, 286, 984, 1024, 1325, 1370
Hpy166II GTNNAC 3 cut(s) 160, 1034, 1411
Hpy188I TCNGA 6 cut(s) 46, 154, 193, 445, 558, 1315
Hpy188III TCNNGA 4 cut(s) 59, 887, 1142, 1329
Hpy8I GTNNAC 3 cut(s) 160, 1034, 1411
HpyAV CCTTC 3 cut(s) 817, 1223, 1357
HpyCH4III ACNGT 4 cut(s) 565, 931, 1031, 1415
HpyCH4IV ACGT 1 cut(s) 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 98
HpyF3I CTNAG 6 cut(s) 117, 320, 444, 504, 972, 1314
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 6 cut(s) 344, 631, 820, 1192, 1208, 1423
HspAI GCGC 1 cut(s) 476
Kzo9I GATC 3 cut(s) 355, 1057, 1117
LmnI GCTCC 7 cut(s) 77, 194, 389, 676, 787, 928, 1051
Lsp1109I GCAGC 1 cut(s) 397
MaeI CTAG 5 cut(s) 210, 756, 836, 851, 1382
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 5 cut(s) 802, 1025, 1154, 1221, 1337
MalI GATC 3 cut(s) 357, 1059, 1119
MboI GATC 3 cut(s) 355, 1057, 1117
MboII GAAGA 4 cut(s) 64, 259, 463, 561
MhlI GDGCHC 3 cut(s) 673, 778, 792
MluCI AATT 8 cut(s) 137, 168, 227, 314, 361, 416, 750, 1111
MlyI GAGTC 3 cut(s) 102, 295, 1033
MmeI TCCRAC 1 cut(s) 950
Mph1103I ATGCAT 3 cut(s) 342, 904, 1425
MroXI GAANNNNTTC 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 410
Mva1269I GAATGC 2 cut(s) 256, 342
MwoI GCNNNNNNNGC 2 cut(s) 29, 98
NdeII GATC 3 cut(s) 355, 1057, 1117
NlaIII CATG 6 cut(s) 344, 631, 820, 1192, 1208, 1423
NlaIV GGNNCC 2 cut(s) 618, 924
NmuCI GTSAC 4 cut(s) 802, 1025, 1154, 1337
NsiI ATGCAT 3 cut(s) 342, 904, 1425
NspI RCATGY 1 cut(s) 1423
PaeI GCATGC 1 cut(s) 1423
PctI GAATGC 2 cut(s) 256, 342
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 5 cut(s) 55, 176, 984, 1325, 1370
PflFI GACNNNGTC 1 cut(s) 565
PkrI GCNGC 1 cut(s) 412
PleI GAGTC 3 cut(s) 102, 294, 1032
PpsI GAGTC 3 cut(s) 102, 294, 1032
PshBI ATTAAT 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 673
PspEI GGTNACC 1 cut(s) 802
PspN4I GGNNCC 2 cut(s) 618, 924
PspPI GGNCC 1 cut(s) 730
PstNI CAGNNNCTG 1 cut(s) 1073
PsyI GACNNNGTC 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 4 cut(s) 871, 940, 1195, 1276
RsaNI GTAC 4 cut(s) 870, 939, 1194, 1275
SacI GAGCTC 1 cut(s) 673
SalI GTCGAC 1 cut(s) 1032
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 3 cut(s) 355, 1057, 1117
Sau96I GGNCC 1 cut(s) 730
ScaI AGTACT 1 cut(s) 1195
SchI GAGTC 3 cut(s) 102, 295, 1033
SduI GDGCHC 3 cut(s) 673, 778, 792
SfcI CTRYAG 1 cut(s) 1407
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
SphI GCATGC 1 cut(s) 1423
Sse9I AATT 8 cut(s) 137, 168, 227, 314, 361, 416, 750, 1111
SsiI CCGC 1 cut(s) 965
SspMI CTAG 5 cut(s) 210, 756, 836, 851, 1382
SstI GAGCTC 1 cut(s) 673
StyI CCWWGG 3 cut(s) 755, 1017, 1159
TaaI ACNGT 4 cut(s) 565, 931, 1031, 1415
TaiI ACGT 1 cut(s) 350
TaqI TCGA 2 cut(s) 234, 1033
TasI AATT 8 cut(s) 137, 168, 227, 314, 361, 416, 750, 1111
TatI WGTACW 2 cut(s) 869, 1193
TfiI GAWTC 5 cut(s) 55, 176, 984, 1325, 1370
TscAI CASTG 6 cut(s) 261, 328, 645, 804, 936, 1420
TseFI GTSAC 4 cut(s) 802, 1025, 1154, 1337
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 4 cut(s) 802, 1025, 1154, 1337
TspDTI ATGAA 2 cut(s) 65, 687
TspGWI ACGGA 1 cut(s) 1363
TspRI CASTG 6 cut(s) 261, 328, 645, 804, 936, 1420
Tth111I GACNNNGTC 1 cut(s) 565
VspI ATTAAT 1 cut(s) 531
XagI CCTNNNNNAGG 1 cut(s) 759
XceI RCATGY 1 cut(s) 1423
XmaJI CCTAGG 1 cut(s) 755
XmiI GTMKAC 3 cut(s) 159, 1033, 1410
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 5 cut(s) 210, 756, 836, 851, 1382
ZrmI AGTACT 1 cut(s) 1195
Zsp2I ATGCAT 3 cut(s) 342, 904, 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.