FvH4_6g05190

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016869
Physical Location & Seq
Forward (+)
2911170 .. 2912195
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05190.t1

Sequence Viewer

Length: 939 bp
ATGGCTGAACCGTTTGTTCAACACATAAACCCATCGTCGTCAACACCAGTAGCAAACCCTGCGGCGGCCGCCTCTTTTTTCCGTTCAATGGAAGATCAACAAATTGTGCTACAAATTGGGCAGCTATCAAGTTCAACACCACCACCACCATCTCATGATGATCAAACCAAATCAGCAACACCAGCATCACACCATGGTACCTTAAACCTCAAGGTAATCGAGCCGATCTTGCTACATATAAACTCATTAACACCACTTCTCAGTAACCAACCAATTAGTACTCAACCAACACCAACACCAACACCAATTAACACCAACACCACCATGCCACCACCACCACCACCACCGTCTGATCCACAGACTGATTTTCCACAACTTTTGAAAGGCGGCACAAAGCACCAAAAGGCACTTAGCAGTGTGGGAAGCCTAGCAAACTTGTTACCTACAGGCACTGTTCTTGCATTCCAAGCCATCACGCCTTCCCTTTCCTACAACGGTAGATGCCAGACCTTCAACAAGTGCCTTGTTGCACTCGTCATGCTAGCTTGTTCTCTCGTCTGCTTCCTCTCTTCCTTCACCGACAGTCTGCAAAACGGGAAGAATTCGAAAGTGTACTATGGCATCGCAACATGCAACGGGCTTCACGTTTTCAACTATGAAGACCCTGAGAATGAAAAGAAAGCTCTTGGTGATAAACTGAAGGATATGAGGATTACATCCGCGGATTATCTTCATGCGTTTGTCTCGGTGTTTGTGTTTCTGATATTTGCTTGTAGCAGCTTAGAGGTGCAGACCTGCTACTTTCCGGCTGAGATAAAAGATCAAATGGAGTATTCGATGGTGATATATTTGCCTTTGGTCGTTGGACTTCTGTCAAGCTTCTTGTTCGCCTATTTCCCTACTAATCGTAGAGGGATTGGCTACACATGCAGAACATGA

Protein Analysis

313

Amino Acids

33.95

Weight (kDa)

6.69

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 139 - 308 5e-54 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016869)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05190
malus_domestica MD04G1047900.v1.1
prunus_persica Prupe.1G204900_v2.0.a1 Prupe.1G205000_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0453841
rosa_laevigata RLG00000025498
rosa_multiflora Rmu_sc0003556.1_g000012
rosa_roxburghii Rroxscaffold_6G00430670
rosa_rugosa Rorug02G0651600
rosa_samantha Rh3AG054000 Rh3BG055400 Rh3CG054700 Rh3DG056000
rosa_wichuraiana Rw3G004150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 803
Acc65I GGTACC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 197
AccII CGCG 1 cut(s) 722
AciI CCGC 6 cut(s) 62, 65, 69, 387, 720, 722
AclWI GGATC 1 cut(s) 347
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 719
AfaI GTAC 3 cut(s) 199, 280, 614
AfiI CCNNNNNNNGG 2 cut(s) 64, 88
AgsI TTSAA 6 cut(s) 20, 87, 135, 382, 514, 652
AluBI AGCT 5 cut(s) 124, 545, 683, 780, 879
AluI AGCT 5 cut(s) 124, 545, 683, 780, 879
Alw26I GTCTC 1 cut(s) 748
AlwI GGATC 1 cut(s) 347
AlwNI CAGNNNCTG 1 cut(s) 452
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 2 cut(s) 121, 777
ApoI RAATTY 1 cut(s) 601
Asp718I GGTACC 1 cut(s) 197
AsuHPI GGTGA 3 cut(s) 568, 701, 853
AsuII TTCGAA 1 cut(s) 605
AsuNHI GCTAGC 1 cut(s) 541
BaeI ACNNNNGTAYC 2 cut(s) 181, 214
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 666
BbvI GCAGC 2 cut(s) 133, 789
BccI CCATC 4 cut(s) 40, 157, 479, 832
BclI TGATCA 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 748
BfaI CTAG 2 cut(s) 428, 542
BfmI CTRYAG 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 803
BisI GCNGC 6 cut(s) 63, 66, 69, 122, 388, 778
BlsI GCNGC 6 cut(s) 64, 67, 70, 123, 389, 779
BmcAI AGTACT 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 199
BmsI GCATC 3 cut(s) 194, 491, 630
BmtI GCTAGC 1 cut(s) 545
BoxI GACNNNNGTC 1 cut(s) 871
BpiI GAAGAC 1 cut(s) 666
Bpu14I TTCGAA 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 193, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 88
Bse1I ACTGG 1 cut(s) 47
BseDI CCNNGG 2 cut(s) 193, 720
BseGI GGATG 1 cut(s) 716
BseLI CCNNNNNNNGG 2 cut(s) 64, 88
BseMII CTCAG 3 cut(s) 274, 657, 801
BseNI ACTGG 1 cut(s) 47
BseX3I CGGCCG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 133, 789
BsgI GTGCAG 1 cut(s) 809
Bsh1236I CGCG 1 cut(s) 722
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 69
BsiSI CCGG 1 cut(s) 806
BslI CCNNNNNNNGG 2 cut(s) 64, 88
BsmAI GTCTC 1 cut(s) 748
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 1 cut(s) 68
Bsp119I TTCGAA 1 cut(s) 605
Bsp143I GATC 5 cut(s) 94, 160, 225, 352, 820
Bsp19I CCATGG 1 cut(s) 193
BspACI CCGC 6 cut(s) 62, 65, 69, 387, 720, 722
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 3 cut(s) 273, 658, 802
BspFNI CGCG 1 cut(s) 722
BspHI TCATGA 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 803
BspOI GCTAGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 347
BspT104I TTCGAA 1 cut(s) 605
BspT107I GGYRCC 1 cut(s) 197
BsrI ACTGG 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 193, 720
BssMI GATC 5 cut(s) 94, 160, 225, 352, 820
BssT1I CCWWGG 1 cut(s) 193
Bst4CI ACNGT 5 cut(s) 12, 348, 454, 497, 584
Bst6I CTCTTC 1 cut(s) 574
BstAPI GCANNNNNTGC 1 cut(s) 59
BstBI TTCGAA 1 cut(s) 605
BstC8I GCNNGC 1 cut(s) 543
BstDEI CTNAG 5 cut(s) 260, 410, 666, 781, 810
BstDSI CCRYGG 2 cut(s) 193, 720
BstF5I GGATG 1 cut(s) 716
BstFNI CGCG 1 cut(s) 722
BstKTI GATC 5 cut(s) 97, 163, 228, 355, 823
BstMAI GTCTC 1 cut(s) 748
BstMBI GATC 5 cut(s) 94, 160, 225, 352, 820
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 6 cut(s) 59, 68, 182, 229, 467, 927
BstNSI RCATGY 2 cut(s) 633, 930
BstPAI GACNNNNGTC 1 cut(s) 871
BstSFI CTRYAG 1 cut(s) 444
BstUI CGCG 1 cut(s) 722
BstV1I GCAGC 2 cut(s) 133, 789
BstV2I GAAGAC 1 cut(s) 666
BstZI CGGCCG 1 cut(s) 66
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 2 cut(s) 193, 720
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 1 cut(s) 716
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 2 cut(s) 421, 450
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 1 cut(s) 543
CaiI CAGNNNCTG 1 cut(s) 452
CciI TCATGA 1 cut(s) 154
CciNI GCGGCCGC 1 cut(s) 66
Cfr42I CCGCGG 1 cut(s) 723
Csp6I GTAC 3 cut(s) 198, 279, 613
CspCI CAANNNNNGTGG 2 cut(s) 360, 395
CviAII CATG 8 cut(s) 155, 194, 325, 538, 630, 734, 927, 936
CviQI GTAC 3 cut(s) 198, 279, 613
DdeI CTNAG 5 cut(s) 260, 410, 666, 781, 810
DpnI GATC 5 cut(s) 96, 162, 227, 354, 822
DpnII GATC 5 cut(s) 94, 160, 225, 352, 820
EaeI YGGCCR 1 cut(s) 66
EagI CGGCCG 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 574
EarI CTCTTC 1 cut(s) 574
EclXI CGGCCG 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 193
Eco52I CGGCCG 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 8 cut(s) 158, 197, 328, 541, 633, 737, 930, 939
FatI CATG 8 cut(s) 154, 193, 324, 537, 629, 733, 926, 935
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 6 cut(s) 63, 66, 69, 122, 388, 778
FokI GGATG 1 cut(s) 703
Fsp4HI GCNGC 6 cut(s) 63, 66, 69, 122, 388, 778
FspBI CTAG 2 cut(s) 428, 542
GluI GCNGC 6 cut(s) 63, 66, 69, 122, 388, 778
HaeIII GGCC 1 cut(s) 68
HapII CCGG 1 cut(s) 806
Hin1II CATG 8 cut(s) 158, 197, 328, 541, 633, 737, 930, 939
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HindIII AAGCTT 1 cut(s) 877
HpaII CCGG 1 cut(s) 806
HphI GGTGA 3 cut(s) 568, 701, 853
Hpy166II GTNNAC 2 cut(s) 42, 613
Hpy188I TCNGA 2 cut(s) 352, 762
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 2 cut(s) 42, 613
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 4 cut(s) 489, 520, 583, 694
HpyCH4III ACNGT 5 cut(s) 12, 348, 454, 497, 584
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 6 cut(s) 461, 530, 589, 633, 790, 930
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 68, 182, 229, 467, 927
HpyF3I CTNAG 5 cut(s) 260, 410, 666, 781, 810
HpySE526I ACGT 1 cut(s) 645
Hsp92II CATG 8 cut(s) 158, 197, 328, 541, 633, 737, 930, 939
KpnI GGTACC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 160
KspI CCGCGG 1 cut(s) 723
Kzo9I GATC 5 cut(s) 94, 160, 225, 352, 820
LpnPI CCDG 8 cut(s) 60, 72, 195, 432, 518, 678, 808, 819
Lsp1109I GCAGC 2 cut(s) 133, 789
LweI GCATC 3 cut(s) 194, 491, 630
MaeI CTAG 2 cut(s) 428, 542
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 2 cut(s) 263, 438
MalI GATC 5 cut(s) 96, 162, 227, 354, 822
MboI GATC 5 cut(s) 94, 160, 225, 352, 820
MboII GAAGA 5 cut(s) 104, 561, 610, 671, 722
MluCI AATT 5 cut(s) 102, 114, 273, 306, 601
MmeI TCCRAC 1 cut(s) 844
MnlI CCTC 6 cut(s) 82, 218, 575, 702, 778, 905
MseI TTAA 3 cut(s) 203, 248, 309
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 1 cut(s) 806
Mva1269I GAATGC 1 cut(s) 461
MvnI CGCG 1 cut(s) 722
MwoI GCNNNNNNNGC 6 cut(s) 59, 68, 182, 229, 467, 927
NcoI CCATGG 1 cut(s) 193
NdeII GATC 5 cut(s) 94, 160, 225, 352, 820
NheI GCTAGC 1 cut(s) 541
NlaIII CATG 8 cut(s) 158, 197, 328, 541, 633, 737, 930, 939
NlaIV GGNNCC 1 cut(s) 199
NotI GCGGCCGC 1 cut(s) 66
NspI RCATGY 2 cut(s) 633, 930
NspV TTCGAA 1 cut(s) 605
PagI TCATGA 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PctI GAATGC 1 cut(s) 461
PkrI GCNGC 6 cut(s) 64, 67, 70, 123, 389, 779
PshAI GACNNNNGTC 1 cut(s) 871
PspN4I GGNNCC 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 199, 280, 614
RsaNI GTAC 3 cut(s) 198, 279, 613
RseI CAYNNNNRTG 1 cut(s) 323
SacII CCGCGG 1 cut(s) 723
SaqAI TTAA 3 cut(s) 203, 248, 309
SatI GCNGC 6 cut(s) 63, 66, 69, 122, 388, 778
Sau3AI GATC 5 cut(s) 94, 160, 225, 352, 820
ScaI AGTACT 1 cut(s) 280
SfaNI GCATC 3 cut(s) 194, 491, 630
SfcI CTRYAG 1 cut(s) 444
Sfr303I CCGCGG 1 cut(s) 723
SfuI TTCGAA 1 cut(s) 605
SgrBI CCGCGG 1 cut(s) 723
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 5 cut(s) 102, 114, 273, 306, 601
SsiI CCGC 6 cut(s) 62, 65, 69, 387, 720, 722
SspMI CTAG 2 cut(s) 428, 542
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 5 cut(s) 12, 348, 454, 497, 584
TaiI ACGT 1 cut(s) 648
TaqI TCGA 3 cut(s) 219, 605, 836
TasI AATT 5 cut(s) 102, 114, 273, 306, 601
TatI WGTACW 2 cut(s) 278, 612
TauI GCSGC 4 cut(s) 65, 68, 71, 390
Tru1I TTAA 3 cut(s) 203, 248, 309
Tru9I TTAA 3 cut(s) 203, 248, 309
TscAI CASTG 2 cut(s) 421, 457
TseI GCWGC 2 cut(s) 121, 777
TspDTI ATGAA 3 cut(s) 672, 687, 722
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 2 cut(s) 421, 457
XapI RAATTY 1 cut(s) 601
XceI RCATGY 2 cut(s) 633, 930
XspI CTAG 2 cut(s) 428, 542
ZrmI AGTACT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.