Rh3DG056000

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0016869
Physical Location & Seq
Forward (+)
3806468 .. 3808133
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG056000.1

Sequence Viewer

Length: 924 bp
ATGGCTGAACCGTTTGTTCAACACATAAACTCATCGTCATCAACACCAGTAGCAAACTCTGCGGCGGCCGCCTCATTAGTCCGGTCAATGGCAGATCAACAGATTGTGTTACAAATTGGACAACTATCAAGTTCAACACCACCACTACCACCACCTCATGATGAACAAACCAAATCAGTAGCACCAGCATTACACCATGGTACCTTAAACCTCAGGGTAACTGAGCCGATTTTGCTACACATAAACTCGTTAACACCACTTCTCAATAACCAACCAATTAGTACTCAATCAACACCAACACCAACACCACCACCACCACCACCAAAAAACCAAGTAACCTCTAATCCACCGACTGATATTGCACAATCTTTGAAAGGCACAAAGCACCAAAAGGCACTTAGCAAGATGGGAAGCCTGGCAAACCTCTTACCCACAGGCACTGTTCTTGCATTCCAAGCCATCACGCCTTCCCTTTCCTACAACGGTAGATGCCACATCTTCAACAAGTACCTTGTTGCATTCGTCATGCTGGCTTGCTCTCTCGTTTGCTTCGTCTCTTCCTTCACCGACAGCCTAGAATATGAGCAGAAAGTGTACTATGGCATTGCAACATTCAACGGGCTTTCAGTTTTCAACTATGAACGTCGTGAGGTTGAAGAAAAAGCTCTTGAGGATAAGCTGAAGTCGCTGAAGATTACACCCAGGGACTATCTTCATGCGTTTCTCTCGGTTTTTGTGTTCTTGATATTTGCTTGTAGCAGCTTAGAGGTGCAGACCTGCTACTTTCCGGCTGGAATAAGAGATCAGTTGGAGTATTCGATGGTGATATATTTGCCTATGGTCGTTGGATTGCTGTCAAGTTTCTTGTTCACCCATTTCCCTACTAACCGTAGAGGGATTGGCTACACATGCACCAGAACATGA

Protein Analysis

307

Amino Acids

33.75

Weight (kDa)

8.48

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 135 - 302 4e-52 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016869)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05190
malus_domestica MD04G1047900.v1.1
prunus_persica Prupe.1G204900_v2.0.a1 Prupe.1G205000_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0453841
rosa_laevigata RLG00000025498
rosa_multiflora Rmu_sc0003556.1_g000012
rosa_roxburghii Rroxscaffold_6G00430670
rosa_rugosa Rorug02G0651600
rosa_samantha Rh3AG054000 Rh3BG055400 Rh3CG054700 Rh3DG056000
rosa_wichuraiana Rw3G004150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 785
Acc65I GGTACC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 3 cut(s) 62, 65, 69
AcoI YGGCCR 1 cut(s) 66
AcuI CTGAAG 2 cut(s) 701, 710
AfaI GTAC 4 cut(s) 202, 283, 509, 596
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 7 cut(s) 20, 135, 373, 502, 616, 634, 656
AjnI CCWGG 2 cut(s) 414, 701
AluBI AGCT 3 cut(s) 665, 679, 762
AluI AGCT 3 cut(s) 665, 679, 762
Alw26I GTCTC 1 cut(s) 559
AlwNI CAGNNNCTG 1 cut(s) 440
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 759
Asp718I GGTACC 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 556, 835, 862
AxyI CCTNAGG 1 cut(s) 212
BaeI ACNNNNGTAYC 2 cut(s) 184, 217
BanI GGYRCC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 771
BccI CCATC 3 cut(s) 400, 467, 814
BciT130I CCWGG 2 cut(s) 416, 703
BcoDI GTCTC 1 cut(s) 559
BfaI CTAG 1 cut(s) 575
BfuAI ACCTGC 1 cut(s) 785
BisI GCNGC 4 cut(s) 63, 66, 69, 760
BlsI GCNGC 4 cut(s) 64, 67, 70, 761
BmcAI AGTACT 1 cut(s) 283
Bme1390I CCNGG 2 cut(s) 416, 703
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 2 cut(s) 416, 703
BmsI GCATC 1 cut(s) 479
BpuEI CTTGAG 1 cut(s) 689
BsaJI CCNNGG 3 cut(s) 196, 701, 702
BsaWI WCCGGW 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 47
Bse21I CCTNAGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 2 cut(s) 416, 703
BseDI CCNNGG 3 cut(s) 196, 701, 702
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 2 cut(s) 213, 226
BseNI ACTGG 1 cut(s) 47
BseX3I CGGCCG 1 cut(s) 66
BseXI GCAGC 1 cut(s) 771
BsgI GTGCAG 1 cut(s) 791
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 200
BsiEI CGRYCG 1 cut(s) 69
BsiSI CCGG 2 cut(s) 82, 788
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 559
BsmBI CGTCTC 1 cut(s) 559
BsmFI GGGAC 1 cut(s) 719
BsmI GAATGC 2 cut(s) 449, 518
BsnI GGCC 1 cut(s) 68
Bsp143I GATC 2 cut(s) 94, 802
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 3 cut(s) 62, 65, 69
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 2 cut(s) 214, 225
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 196, 701, 702
BssMI GATC 2 cut(s) 94, 802
BssT1I CCWWGG 1 cut(s) 196
Bst2UI CCWGG 2 cut(s) 416, 703
Bst4CI ACNGT 4 cut(s) 12, 442, 485, 890
Bst6I CTCTTC 1 cut(s) 562
BstAPI GCANNNNNTGC 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 531, 535
BstDEI CTNAG 4 cut(s) 212, 222, 398, 763
BstDSI CCRYGG 1 cut(s) 196
BstKTI GATC 2 cut(s) 97, 805
BstMAI GTCTC 1 cut(s) 559
BstMBI GATC 2 cut(s) 94, 802
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 6 cut(s) 59, 68, 232, 455, 685, 909
BstNI CCWGG 2 cut(s) 416, 703
BstNSI RCATGY 1 cut(s) 912
BstSCI CCNGG 2 cut(s) 414, 701
BstV1I GCAGC 1 cut(s) 771
BstZI CGGCCG 1 cut(s) 66
Bsu36I CCTNAGG 1 cut(s) 212
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 438
BveI ACCTGC 1 cut(s) 785
Cac8I GCNNGC 2 cut(s) 531, 535
CaiI CAGNNNCTG 1 cut(s) 440
CciI TCATGA 1 cut(s) 157
CciNI GCGGCCGC 1 cut(s) 66
Csp6I GTAC 4 cut(s) 201, 282, 508, 595
CviAII CATG 6 cut(s) 158, 197, 526, 716, 909, 921
CviQI GTAC 4 cut(s) 201, 282, 508, 595
DdeI CTNAG 4 cut(s) 212, 222, 398, 763
DpnI GATC 2 cut(s) 96, 804
DpnII GATC 2 cut(s) 94, 802
EaeI YGGCCR 1 cut(s) 66
EagI CGGCCG 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 562
EclXI CGGCCG 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 196
Eco52I CGGCCG 1 cut(s) 66
Eco57I CTGAAG 2 cut(s) 701, 710
Eco81I CCTNAGG 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 414, 701
EcoT14I CCWWGG 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 559
FaeI CATG 6 cut(s) 161, 200, 529, 719, 912, 924
FaqI GGGAC 1 cut(s) 719
FatI CATG 6 cut(s) 157, 196, 525, 715, 908, 920
Fnu4HI GCNGC 4 cut(s) 63, 66, 69, 760
Fsp4HI GCNGC 4 cut(s) 63, 66, 69, 760
FspBI CTAG 1 cut(s) 575
GluI GCNGC 4 cut(s) 63, 66, 69, 760
HaeIII GGCC 1 cut(s) 68
HapII CCGG 2 cut(s) 82, 788
Hin1II CATG 6 cut(s) 161, 200, 529, 719, 912, 924
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HpaI GTTAAC 1 cut(s) 252
HpaII CCGG 2 cut(s) 82, 788
HphI GGTGA 3 cut(s) 556, 835, 862
Hpy166II GTNNAC 3 cut(s) 252, 595, 870
Hpy188III TCNNGA 4 cut(s) 158, 647, 668, 742
Hpy8I GTNNAC 3 cut(s) 252, 595, 870
Hpy99I CGWCG 1 cut(s) 648
HpyAV CCTTC 2 cut(s) 477, 571
HpyCH4III ACNGT 4 cut(s) 12, 442, 485, 890
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 6 cut(s) 362, 449, 518, 608, 772, 912
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 68, 232, 455, 685, 909
HpyF3I CTNAG 4 cut(s) 212, 222, 398, 763
HpySE526I ACGT 1 cut(s) 643
Hsp92II CATG 6 cut(s) 161, 200, 529, 719, 912, 924
KpnI GGTACC 1 cut(s) 204
KspAI GTTAAC 1 cut(s) 252
Kzo9I GATC 2 cut(s) 94, 802
Lsp1109I GCAGC 1 cut(s) 771
LweI GCATC 1 cut(s) 479
MaeI CTAG 1 cut(s) 575
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 3 cut(s) 108, 217, 334
MalI GATC 2 cut(s) 96, 804
MboI GATC 2 cut(s) 94, 802
MboII GAAGA 5 cut(s) 490, 549, 668, 703, 704
MluCI AATT 2 cut(s) 114, 276
MmeI TCCRAC 2 cut(s) 789, 826
MnlI CCTC 9 cut(s) 82, 165, 221, 349, 434, 643, 664, 760, 887
MseI TTAA 2 cut(s) 206, 251
MspI CCGG 2 cut(s) 82, 788
MspR9I CCNGG 2 cut(s) 416, 703
Mva1269I GAATGC 2 cut(s) 449, 518
MvaI CCWGG 2 cut(s) 416, 703
MwoI GCNNNNNNNGC 6 cut(s) 59, 68, 232, 455, 685, 909
NcoI CCATGG 1 cut(s) 196
NdeII GATC 2 cut(s) 94, 802
NlaIII CATG 6 cut(s) 161, 200, 529, 719, 912, 924
NlaIV GGNNCC 1 cut(s) 202
NotI GCGGCCGC 1 cut(s) 66
NspI RCATGY 1 cut(s) 912
PagI TCATGA 1 cut(s) 157
PasI CCCWGGG 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PctI GAATGC 2 cut(s) 449, 518
PkrI GCNGC 4 cut(s) 64, 67, 70, 761
Psp6I CCWGG 2 cut(s) 414, 701
PspGI CCWGG 2 cut(s) 414, 701
PspN4I GGNNCC 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 440
RsaI GTAC 4 cut(s) 202, 283, 509, 596
RsaNI GTAC 4 cut(s) 201, 282, 508, 595
SaqAI TTAA 2 cut(s) 206, 251
SatI GCNGC 4 cut(s) 63, 66, 69, 760
Sau3AI GATC 2 cut(s) 94, 802
ScaI AGTACT 1 cut(s) 283
ScrFI CCNGG 2 cut(s) 416, 703
SfaNI GCATC 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 2 cut(s) 114, 276
SsiI CCGC 3 cut(s) 62, 65, 69
SspMI CTAG 1 cut(s) 575
StyD4I CCNGG 2 cut(s) 414, 701
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 4 cut(s) 12, 442, 485, 890
TaiI ACGT 1 cut(s) 646
TaqI TCGA 1 cut(s) 818
TasI AATT 2 cut(s) 114, 276
TatI WGTACW 2 cut(s) 281, 594
TauI GCSGC 3 cut(s) 65, 68, 71
Tru1I TTAA 2 cut(s) 206, 251
Tru9I TTAA 2 cut(s) 206, 251
TscAI CASTG 1 cut(s) 445
TseI GCWGC 1 cut(s) 759
TspDTI ATGAA 3 cut(s) 177, 654, 704
TspRI CASTG 1 cut(s) 445
XceI RCATGY 1 cut(s) 912
XspI CTAG 1 cut(s) 575
ZrmI AGTACT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.